P. Dehghanpisheh, A. Jahandideh, A. Asghari, P. Mortazavi, M. Ghorannevis
Objectives: Patients with diabetes often suffer from chronic wounds which can occur due to the impairment of wound healing in these patients. Scientists have been trying to address this issue by using wide spectrum of antibiotics and drugs. However, overusing antibiotics in recent years has led to antibiotic resistant crisis; therefore, there is an urgent need to develop new approaches in order for controlling microbial infections in diabetic patients. This study aimed to determine if cold plasma was effective in wound healing in the infectious diabetic conditions. Material and Methods: In this experimental study, 40 adult male rats with diabetes infected with Staphylococcus aureus were included. The animals were randomly divided into two groups of 20 rats and four sub-groups (i.e., day 3rd, day 7th, day 14th, and day 21st). The rats in the treatment group were exposed to helium plasma irradiated for 3 minutes per day for 21 days. Wound healing in the samples was evaluated using five healing indexes including epithelium formation, Inflammatory cells, new vascularization, fibroblast, and collagen formation. Results: A significant difference (P<0.05) was found between the indexes in the plasma radiation group compared to the control group. The results showed that the speed of wound healing in the group treated with cold helium gas plasma was higher than that in the control group. Collagen formation was always completed faster in the helium plasma group, indicating the positive effects of helium plasma on infected diabetic wounds. Conclusions: Helium plasma was remarkable effective in healing wound and controlling infections in diabetic rats.
{"title":"Effects of Cold Atmospheric Plasma on Infectious Diabetic Wound Healing in Rat Models","authors":"P. Dehghanpisheh, A. Jahandideh, A. Asghari, P. Mortazavi, M. Ghorannevis","doi":"10.34172/cjmb.2023.14","DOIUrl":"https://doi.org/10.34172/cjmb.2023.14","url":null,"abstract":"Objectives: Patients with diabetes often suffer from chronic wounds which can occur due to the impairment of wound healing in these patients. Scientists have been trying to address this issue by using wide spectrum of antibiotics and drugs. However, overusing antibiotics in recent years has led to antibiotic resistant crisis; therefore, there is an urgent need to develop new approaches in order for controlling microbial infections in diabetic patients. This study aimed to determine if cold plasma was effective in wound healing in the infectious diabetic conditions. Material and Methods: In this experimental study, 40 adult male rats with diabetes infected with Staphylococcus aureus were included. The animals were randomly divided into two groups of 20 rats and four sub-groups (i.e., day 3rd, day 7th, day 14th, and day 21st). The rats in the treatment group were exposed to helium plasma irradiated for 3 minutes per day for 21 days. Wound healing in the samples was evaluated using five healing indexes including epithelium formation, Inflammatory cells, new vascularization, fibroblast, and collagen formation. Results: A significant difference (P<0.05) was found between the indexes in the plasma radiation group compared to the control group. The results showed that the speed of wound healing in the group treated with cold helium gas plasma was higher than that in the control group. Collagen formation was always completed faster in the helium plasma group, indicating the positive effects of helium plasma on infected diabetic wounds. Conclusions: Helium plasma was remarkable effective in healing wound and controlling infections in diabetic rats.","PeriodicalId":43540,"journal":{"name":"Crescent Journal of Medical and Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2023-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46081378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Amir Eftekhari Milani, Bahareh Javani, Mohamadhosein Ahoor, Mehdi Abdi Shahshahani, Fereshteh Farhadi
Objectives: This study aimed to investigate the effects of bevacizumab (an anti-VEGF antibody) on the structure of retina such as the thickness of the nerve fiber layer and macula with optical coherence tomography (OCT) in retinopathy of prematurity (ROP) patients. Materials and Methods: This cross-sectional study was conducted in 2021-2022 in Tabriz, Iran to investigate preterm children aged 4-6 years. To this end, a total of 300 eyes of 150 children were assigned to three groups and then were examined by performing Fundus examination of retina, eye refraction test, and OCT imaging. The first group included 100 eyes with a history of ROP and treated with bevacizumab, the second group consisted of 100 eyes with a history of ROP but improved spontaneously, and the third group comprised 100 eyes of preterm children without a history of ROP. Results: Foveal thickness was 235.46 ± 11 µm in the group receiving bevacizumab, 267.70 ± 12 µm in the group with spontaneous improvement of ROP, and 269.48 ± 10 µm in the group with no ROP. Therefore, the mean thickness of the fovea in the group receiving bevacizumab was significantly lower than that in the other two groups. In the group receiving bevacizumab, moreover, the thickness of the nerve layer of the retina was less than that in the other two groups (P<0.005). Conclusions: In sum, a greater understanding of the development of premature fovea was achieved, which may have facilitated identifying the possible clinical and long-term effects of these abnormal changes on adulthood visual acuity. It was recommended that further studies should be carried out to compare laser-treated eyes in order for expanding the scientific understanding of the development of premature fovea and other aspects of ROP, which may have decreased the preventable blindness rate.
{"title":"Foveal and Retinal Nerve Fiber Layer Thickness in Children With a History of Retinopathy of Prematurity Treated With Bevacizumab: A Cross-sectional Study","authors":"Amir Eftekhari Milani, Bahareh Javani, Mohamadhosein Ahoor, Mehdi Abdi Shahshahani, Fereshteh Farhadi","doi":"10.34172/cjmb.2023.27","DOIUrl":"https://doi.org/10.34172/cjmb.2023.27","url":null,"abstract":"Objectives: This study aimed to investigate the effects of bevacizumab (an anti-VEGF antibody) on the structure of retina such as the thickness of the nerve fiber layer and macula with optical coherence tomography (OCT) in retinopathy of prematurity (ROP) patients. Materials and Methods: This cross-sectional study was conducted in 2021-2022 in Tabriz, Iran to investigate preterm children aged 4-6 years. To this end, a total of 300 eyes of 150 children were assigned to three groups and then were examined by performing Fundus examination of retina, eye refraction test, and OCT imaging. The first group included 100 eyes with a history of ROP and treated with bevacizumab, the second group consisted of 100 eyes with a history of ROP but improved spontaneously, and the third group comprised 100 eyes of preterm children without a history of ROP. Results: Foveal thickness was 235.46 ± 11 µm in the group receiving bevacizumab, 267.70 ± 12 µm in the group with spontaneous improvement of ROP, and 269.48 ± 10 µm in the group with no ROP. Therefore, the mean thickness of the fovea in the group receiving bevacizumab was significantly lower than that in the other two groups. In the group receiving bevacizumab, moreover, the thickness of the nerve layer of the retina was less than that in the other two groups (P<0.005). Conclusions: In sum, a greater understanding of the development of premature fovea was achieved, which may have facilitated identifying the possible clinical and long-term effects of these abnormal changes on adulthood visual acuity. It was recommended that further studies should be carried out to compare laser-treated eyes in order for expanding the scientific understanding of the development of premature fovea and other aspects of ROP, which may have decreased the preventable blindness rate.","PeriodicalId":43540,"journal":{"name":"Crescent Journal of Medical and Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135340051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Samaneh Noroozi, M. Karimi, A. Vasheghani Farahani, N. Omidi, A. Zargaran, S. Soleymani, F. Alaeddini, H. Rezaeizadeh
Objectives: Treatment of cardiac syndrome X (CSX) remains a major challenge for conventional medicine. In this regard, developing new natural treatments could be an alternative choice. This study was planned to appraise the efficacy of chamomile-lemon balm syrup on chest pain and quality of life in patients with conventional drug-resistant CSX. Materials and Methods: 29 participants with conventional drug-resistant were enrolled in a single-arm clinical trial, and 14 participants completed the study protocol. Chamomile (Matricaria chamomilla L.)-lemon balm (Melissa officinalis L.) syrup was provided for the treatment for the 90-day study period. All conventional treatments of CSX remained unchanged. Efficacy assessment included Seattle Angina Questionnaires (SAQ), 36-item short form survey, and the Hospital Anxiety and Depression Scale (HADS). Results: An improvement was observed in the total score of all questionnaires with statistically significant changes over time of the study (P<0.001). Conclusions: Chamomile-lemon balm syrup showed promising results in improving effect on angina symptoms, quality of life, and anxiety and depression in the patients with drug-resistant CSX. However, a placebo-controlled trial should be performed to verify these data.
{"title":"Efficacy of Chamomile-Lemon Balm Syrup in Patients With Conventional Drug-Resistant Cardiac Syndrome X: A Single-Arm Clinical Trial","authors":"Samaneh Noroozi, M. Karimi, A. Vasheghani Farahani, N. Omidi, A. Zargaran, S. Soleymani, F. Alaeddini, H. Rezaeizadeh","doi":"10.34172/cjmb.2023.12","DOIUrl":"https://doi.org/10.34172/cjmb.2023.12","url":null,"abstract":"Objectives: Treatment of cardiac syndrome X (CSX) remains a major challenge for conventional medicine. In this regard, developing new natural treatments could be an alternative choice. This study was planned to appraise the efficacy of chamomile-lemon balm syrup on chest pain and quality of life in patients with conventional drug-resistant CSX. Materials and Methods: 29 participants with conventional drug-resistant were enrolled in a single-arm clinical trial, and 14 participants completed the study protocol. Chamomile (Matricaria chamomilla L.)-lemon balm (Melissa officinalis L.) syrup was provided for the treatment for the 90-day study period. All conventional treatments of CSX remained unchanged. Efficacy assessment included Seattle Angina Questionnaires (SAQ), 36-item short form survey, and the Hospital Anxiety and Depression Scale (HADS). Results: An improvement was observed in the total score of all questionnaires with statistically significant changes over time of the study (P<0.001). Conclusions: Chamomile-lemon balm syrup showed promising results in improving effect on angina symptoms, quality of life, and anxiety and depression in the patients with drug-resistant CSX. However, a placebo-controlled trial should be performed to verify these data.","PeriodicalId":43540,"journal":{"name":"Crescent Journal of Medical and Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2023-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41465052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hamed Soleimani Samarkhazan, S. Alizadeh, Ziba Majidi, Z. Kashani Khatib, M. Naderi
Objectives: Congenital prothrombin (factor II) deficiency is an inherited rare bleeding disorder with an autosomal recessive manner. The prevalence of this disorder is about one in 2000000 people in general population, but it is more common in areas with a high rate of consanguinity. To date, there is no report on the absence of prothrombin, which is a life-threating disorder. Considering the importance of factor II in body homeostasis, this study aimed to find any possible mutation of coagulation factor II codons in patients with inherited factor II deficiency in southeastern Iran. Materials andMethods: This study was conducted on 12 patients with inherited deficiency of prothrombin. Early diagnosis was based on clinical symptoms, laboratory evaluation, and family history. Then, the function level of prothrombin was measured, the initial diagnosis of disease was confirmed, and polymerase chain reaction (PCR) analysis was performed. Finally, gene sequencing and genotyping of factor II was done. Results: Molecular analysis indicated a point mutation in exon 7 in three patients and a frameshift mutation in exon 14 due to addition of a thymine base at position 1760-1761 in one patient, both of which have been reported for the first time. Conclusions: Molecular methods performed on patients from Southeastern Iranian population in terms of coagulation factor II deficiency revealed a substitution mutation in exon 7 in three patients and a frameshift mutation in exon 14 in one patient, both of which were reported for the first time. Considering the significant difference between the clinical symptoms of the present study and previous studies, probably the type of mutations reported in this study (for the first time) caused these clinical symptoms, but statistical studies did not show any relationship between the type of mutation and the occurrence of clinical symptoms. And it needs more investigations on more patients, with a larger population.
{"title":"Determination of Factor II Codons Genotype in Southeastern Iranian Patients With Hereditary Deficiency of Factor II","authors":"Hamed Soleimani Samarkhazan, S. Alizadeh, Ziba Majidi, Z. Kashani Khatib, M. Naderi","doi":"10.34172/cjmb.2023.10","DOIUrl":"https://doi.org/10.34172/cjmb.2023.10","url":null,"abstract":"Objectives: Congenital prothrombin (factor II) deficiency is an inherited rare bleeding disorder with an autosomal recessive manner. The prevalence of this disorder is about one in 2000000 people in general population, but it is more common in areas with a high rate of consanguinity. To date, there is no report on the absence of prothrombin, which is a life-threating disorder. Considering the importance of factor II in body homeostasis, this study aimed to find any possible mutation of coagulation factor II codons in patients with inherited factor II deficiency in southeastern Iran. Materials andMethods: This study was conducted on 12 patients with inherited deficiency of prothrombin. Early diagnosis was based on clinical symptoms, laboratory evaluation, and family history. Then, the function level of prothrombin was measured, the initial diagnosis of disease was confirmed, and polymerase chain reaction (PCR) analysis was performed. Finally, gene sequencing and genotyping of factor II was done. Results: Molecular analysis indicated a point mutation in exon 7 in three patients and a frameshift mutation in exon 14 due to addition of a thymine base at position 1760-1761 in one patient, both of which have been reported for the first time. Conclusions: Molecular methods performed on patients from Southeastern Iranian population in terms of coagulation factor II deficiency revealed a substitution mutation in exon 7 in three patients and a frameshift mutation in exon 14 in one patient, both of which were reported for the first time. Considering the significant difference between the clinical symptoms of the present study and previous studies, probably the type of mutations reported in this study (for the first time) caused these clinical symptoms, but statistical studies did not show any relationship between the type of mutation and the occurrence of clinical symptoms. And it needs more investigations on more patients, with a larger population.","PeriodicalId":43540,"journal":{"name":"Crescent Journal of Medical and Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2023-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46396073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Z. Asadi, A. Shahcheraghi, Leila Zare, M. Gharehbaglou
Objectives: According to the available evidence, changes in the design of medical centers, which can create a more comfortable, pleasant, and natural environment, have reduced the patient tensions and increased their satisfaction with the quality of treatment in the given centers. The present study aimed to investigate the effect of the architecture and design of a supportive care environment on the treatment process in hospitals adopting a qualitative study design. Materials and Methods: This qualitative study with a phenomenological approach was conducted to investigate the effect of an optimal supportive care environment in hospitals based on the experiences and opinions of experts, university professors of architecture, and doctors in hospitals. To this end, experts were interviewed qualitatively and, then, eight main themes as well as 22 sub-themes were classified by performing content analysis. Furthermore, the effect of the environment on the patients, treatment control length, blood pressure control, and subjective nature of the disease’s impact, as well as the effect of social relations on the vital signs, comprehensiveness and acceptability, place of using the supportive care model and a completely mechanical attitude towards humans in the modern period were all evaluated. Results: The effect of the optimal supportive care environment on the patients’ treatment processes was confirmed by qualitatively reviewing the experts’ viewpoints and collecting comprehensive information from other sources. Conclusions: In sum, it was recommended that that the heads of the hospitals should consider the concepts of optimal supportive care environment when designing and/or reconstructing hospitals.
{"title":"The Effect of Supportive Care Environment on the Treatment Process in Hospitals: A Qualitative Study","authors":"Z. Asadi, A. Shahcheraghi, Leila Zare, M. Gharehbaglou","doi":"10.34172/cjmb.2023.13","DOIUrl":"https://doi.org/10.34172/cjmb.2023.13","url":null,"abstract":"Objectives: According to the available evidence, changes in the design of medical centers, which can create a more comfortable, pleasant, and natural environment, have reduced the patient tensions and increased their satisfaction with the quality of treatment in the given centers. The present study aimed to investigate the effect of the architecture and design of a supportive care environment on the treatment process in hospitals adopting a qualitative study design. Materials and Methods: This qualitative study with a phenomenological approach was conducted to investigate the effect of an optimal supportive care environment in hospitals based on the experiences and opinions of experts, university professors of architecture, and doctors in hospitals. To this end, experts were interviewed qualitatively and, then, eight main themes as well as 22 sub-themes were classified by performing content analysis. Furthermore, the effect of the environment on the patients, treatment control length, blood pressure control, and subjective nature of the disease’s impact, as well as the effect of social relations on the vital signs, comprehensiveness and acceptability, place of using the supportive care model and a completely mechanical attitude towards humans in the modern period were all evaluated. Results: The effect of the optimal supportive care environment on the patients’ treatment processes was confirmed by qualitatively reviewing the experts’ viewpoints and collecting comprehensive information from other sources. Conclusions: In sum, it was recommended that that the heads of the hospitals should consider the concepts of optimal supportive care environment when designing and/or reconstructing hospitals.","PeriodicalId":43540,"journal":{"name":"Crescent Journal of Medical and Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2023-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48462850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
With the aim of early diagnosis of cancer and considerable advancements in its treatment, 5-year survival rate was increased 80% for young oncological patients in recent years (1). Oncological therapies including extensive chemotherapy and radiotherapy are known to be highly ovotoxic and frequently result in early ovarian failure and subsequent infertility (2). Children who get chemotherapy or radiotherapy are at approximately 30% risk of developing gonadal dysfunction (3). Chemotherapeutic drugs, particularly alkylating agents, alter DNA replication and cell division, massively activate the primordial follicles, and cause follicular atresia (4). Radiation is harmful to oocytes and even a dose of less than 2 Gy can destroy 50% of primordial follicles. Options for preserving fertility in cancer patients include in vitro maturation of oocytes, cryopreservation of oocyte, sperm, embryo or ovarian tissue, and fertility-preserving surgery (5). Single oocyte or embryo freezing with a conventional ovarian stimulation protocol continues at least two weeks and this may delay the initiation of adjuvant chemotherapy or radiotherapy. Therefore, random start stimulation of ovary at any time during menstrual cycle is essential for onco-fertility patients. Dual stimulation also provides more oocytes and embryos in a short time by capturing 2-3 follicle waves in the same menstrual period. In conclusion, infertility due to cancer treatment is not an inevitable end. Fertility preservative procedures before the initiation of oncological treatments, may help these patients to get pregnant after cancer therapies.
{"title":"Is Fertility a Dream for Cancer Patients?","authors":"Derya Özdemir Taş, Z. Kurdoğlu","doi":"10.34172/cjmb.2023.01","DOIUrl":"https://doi.org/10.34172/cjmb.2023.01","url":null,"abstract":"With the aim of early diagnosis of cancer and considerable advancements in its treatment, 5-year survival rate was increased 80% for young oncological patients in recent years (1). Oncological therapies including extensive chemotherapy and radiotherapy are known to be highly ovotoxic and frequently result in early ovarian failure and subsequent infertility (2). Children who get chemotherapy or radiotherapy are at approximately 30% risk of developing gonadal dysfunction (3). Chemotherapeutic drugs, particularly alkylating agents, alter DNA replication and cell division, massively activate the primordial follicles, and cause follicular atresia (4). Radiation is harmful to oocytes and even a dose of less than 2 Gy can destroy 50% of primordial follicles. Options for preserving fertility in cancer patients include in vitro maturation of oocytes, cryopreservation of oocyte, sperm, embryo or ovarian tissue, and fertility-preserving surgery (5). Single oocyte or embryo freezing with a conventional ovarian stimulation protocol continues at least two weeks and this may delay the initiation of adjuvant chemotherapy or radiotherapy. Therefore, random start stimulation of ovary at any time during menstrual cycle is essential for onco-fertility patients. Dual stimulation also provides more oocytes and embryos in a short time by capturing 2-3 follicle waves in the same menstrual period. In conclusion, infertility due to cancer treatment is not an inevitable end. Fertility preservative procedures before the initiation of oncological treatments, may help these patients to get pregnant after cancer therapies.","PeriodicalId":43540,"journal":{"name":"Crescent Journal of Medical and Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2023-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48204211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nabiollah Ahmadi, A. Khaki, P. Khodarahmi, J. Baharara, S. Zakerbostanabadi
Objectives: This study aimed to evaluate the effect of anthocyanin on oxidative stress, sperm, and testis structure in diabetic rats induced by streptozotocin (STZ). Materials and Methods: In this experimental research, diabetes was induced by a single intraperitoneal injection of STZ (50 mg/ kg). A total of 64 rats were assigned into four groups as follows: a control group, a diabetic control group, a diabetic group daily administrated with anthocyanin at a dose of 100 mg/kg, and a healthy group daily administrated with anthocyanin for 56 days. After intervention, all the rats were anesthetized, their blood samples were taken, and the serum levels of insulin, glucose, and oxidative stress markers were measured. Finally, the testicles were removed and histological parameters were assessed. Results: Treating diabetic rats with anthocyanin significantly improved the testis tissue damage, glucose, and insulin plasma levels (P=0.001). Furthermore, the level of malondialdehyde (MDA) was up-surged and the serum levels of superoxide dismutase (SOD) and catalase (CAT) were reduced (P=0.001). Also, anthocyanin administration (100 mg/kg BW) significantly rectified these parameters (P<0.05). Conclusions: Our results confirmed the antioxidant role of anthocyanin in improving the sperm parameters and testicular oxidative damage caused by diabetes.
{"title":"Effect of Anthocyanin on Oxidative Stress and Testis Structure in Streptozotocin-Induced Diabetic Rats","authors":"Nabiollah Ahmadi, A. Khaki, P. Khodarahmi, J. Baharara, S. Zakerbostanabadi","doi":"10.34172/cjmb.2023.09","DOIUrl":"https://doi.org/10.34172/cjmb.2023.09","url":null,"abstract":"Objectives: This study aimed to evaluate the effect of anthocyanin on oxidative stress, sperm, and testis structure in diabetic rats induced by streptozotocin (STZ). Materials and Methods: In this experimental research, diabetes was induced by a single intraperitoneal injection of STZ (50 mg/ kg). A total of 64 rats were assigned into four groups as follows: a control group, a diabetic control group, a diabetic group daily administrated with anthocyanin at a dose of 100 mg/kg, and a healthy group daily administrated with anthocyanin for 56 days. After intervention, all the rats were anesthetized, their blood samples were taken, and the serum levels of insulin, glucose, and oxidative stress markers were measured. Finally, the testicles were removed and histological parameters were assessed. Results: Treating diabetic rats with anthocyanin significantly improved the testis tissue damage, glucose, and insulin plasma levels (P=0.001). Furthermore, the level of malondialdehyde (MDA) was up-surged and the serum levels of superoxide dismutase (SOD) and catalase (CAT) were reduced (P=0.001). Also, anthocyanin administration (100 mg/kg BW) significantly rectified these parameters (P<0.05). Conclusions: Our results confirmed the antioxidant role of anthocyanin in improving the sperm parameters and testicular oxidative damage caused by diabetes.","PeriodicalId":43540,"journal":{"name":"Crescent Journal of Medical and Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2023-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41978651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mina Naghi Jafarabadi, Maedeh Ahmadi Dastjerdi, B. Hossein Rashidi, M. Shariat, F. Haghollahi
Objectives: This study aimed to determine the relationships between endometrial compaction and pregnancy outcome in patients receiving artificial endometrial preparation for frozen embryo transfer (FET) cycles. Materials and Methods: This prospective cohort study was performed in a university-affiliated fertility clinic from March 2020 to March 2021. The eligible women undergoing their first or second FET cycle and having the top grading cleavage stage embryos were enrolled. All patients received the same endometrial preparation regime. The alteration in endometrial thickness (EMT) between the day of progesterone initiation and the day of embryo transfer (ET) was measured using consecutive transvaginal sonography. The patients were divided into three groups based on the percentage of endometrial compaction (i.e., the difference of EMT at end of the estrogen-only phase and after three days of progesterone administration (ET day) divided by the EMT on the terminal day of the estrogen-only exposure). Results: Overall, 300 eligible women were evaluated and only 27.3% (82/300) of the studied cycles showed ≥5% compaction, whereas 72.6% (218/300) either expanded or showed minimal compaction. The clinical and ongoing pregnancy rates in group 2 (any expansion) were significantly higher than those in groups 1 and 3 (P=0.002 and P=0.01, respectively). Multivariable logistic regression test indicated that the cycles with any expansion in ET were independently associated with 3.1 times improvement in clinical pregnancy rate in comparison to those with any compaction (P=0.002). Conclusion: Gross endometrial compaction occurred in one-third of FET cycles with no significant positive effect on pregnancy outcomes after cleavage-stage ET.
{"title":"Does Endometrial Compaction Predict Clinical Pregnancy Rate after Cleavage Stage Frozen Embryo Transfer?","authors":"Mina Naghi Jafarabadi, Maedeh Ahmadi Dastjerdi, B. Hossein Rashidi, M. Shariat, F. Haghollahi","doi":"10.34172/cjmb.2023.22","DOIUrl":"https://doi.org/10.34172/cjmb.2023.22","url":null,"abstract":"Objectives: This study aimed to determine the relationships between endometrial compaction and pregnancy outcome in patients receiving artificial endometrial preparation for frozen embryo transfer (FET) cycles. Materials and Methods: This prospective cohort study was performed in a university-affiliated fertility clinic from March 2020 to March 2021. The eligible women undergoing their first or second FET cycle and having the top grading cleavage stage embryos were enrolled. All patients received the same endometrial preparation regime. The alteration in endometrial thickness (EMT) between the day of progesterone initiation and the day of embryo transfer (ET) was measured using consecutive transvaginal sonography. The patients were divided into three groups based on the percentage of endometrial compaction (i.e., the difference of EMT at end of the estrogen-only phase and after three days of progesterone administration (ET day) divided by the EMT on the terminal day of the estrogen-only exposure). Results: Overall, 300 eligible women were evaluated and only 27.3% (82/300) of the studied cycles showed ≥5% compaction, whereas 72.6% (218/300) either expanded or showed minimal compaction. The clinical and ongoing pregnancy rates in group 2 (any expansion) were significantly higher than those in groups 1 and 3 (P=0.002 and P=0.01, respectively). Multivariable logistic regression test indicated that the cycles with any expansion in ET were independently associated with 3.1 times improvement in clinical pregnancy rate in comparison to those with any compaction (P=0.002). Conclusion: Gross endometrial compaction occurred in one-third of FET cycles with no significant positive effect on pregnancy outcomes after cleavage-stage ET.","PeriodicalId":43540,"journal":{"name":"Crescent Journal of Medical and Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2023-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43003811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Hamidi, A. Tofighi, Javad Toluei Azar, A. Khaki, M. Razi
Objectives: This study aimed to evaluate the effect of crocin and treadmill exercise on oxidative stress, sperm parameters, and testis structure in streptozotocin (STZ)-induced diabetic rats. Materials and Methods: In this experimental study, 64 diabetic rats induced by a single intraperitoneal (IP) injection of STZ (50 mg/ kg) were assigned to the following groups (n=8/each): healthy control group, diabetic control group, diabetic group treated daily with crocin at a dose of 50 mg/kg, and one healthy group receiving daily crocin at a dose of 50 mg/kg for 56 days. Some groups such as the controls and diabetics exercised with treadmill, as well as the healthy and diabetic groups performed daily exercise with treadmill and crocin. After crocin treatment, all rats were anesthetized, their blood samples were taken, and the serum level of insulin, glucose, and oxidative stress markers were measured. Finally, the testicles and epididymis were removed and sperm parameters were assessed. Results: Treatment of diabetic rats with crocin along with treadmill exercise significantly replaced the testicular tissue damage, sperm parameters, glucose, and insulin plasma levels (P=0.001). In diabetic rats, moreover, the level of malondialdehyde (MDA) was increased and the levels of superoxide dismutase (SOD) and catalase (CAT) enzymes activity were reduced in the testicular tissue (P=0.001). Crocin administration (50 mg/kg BW) and exercise significantly improved these parameters (P<0.05). Conclusions: Our results confirm the antioxidant role of crocin and the positive role of treadmill exercise in improving the sperm parameters and testicular oxidative damage caused by diabetes.
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M. Mashhadi, A. Saeidi, M. Tansaz, Soodabeh Bioos, M. Tabarrai, -. ZahraDarvish-Mofrad, Kashani, Ali Montazeri, M. Saberi, M. Mojahedi, R. Mokaberinejad
Objectives: According to Persian medicine (PM), the uterus is an important organ in women, responsible for purifying the blood and nourishing the fetus. Each organ has a specific temperament distinct from the whole-body temperament based on PM. Dystemperament occurs when body or organ Mizaj (Persian word for temperament) deviates from what is considered normal, resulting in malfunction. Many gynecological disorders described in PM and conventional medicine, including infertility, recurrent miscarriage, oligomenorrhea/amenorrhea, hypermenorrhea, vaginitis, cervicitis, urinary incontinence, and pelvic pain, are considered to be associated with uterine dystemperaments. Hence, proper management of such disorders requires precise diagnosis and treatment of uterine dystemperaments. Accordingly, this review study aimed to collect and categorize these symptoms from PM texts and other relevant articles. Methods: In this study, we reviewed 10 well-known PM references, including Canon in Medicine (Al-Qanun Fi al-Teb), the Great Panacea (Exir-e A’zam), the Treasure of Kharazmshah (Zakhireh Kharazmshahi), the Perfect Book of the Art of Medicine (Kamel alSana’a al-Tebbiya), Akbari’s Medicine (Tebb-E-Akbari), Gharshi’s Commentary on Canon of Medicine (Sharh-e Qarshi bar Qanun), Commentary on Hippocrates’ Aphorisms (Sharh-e Fosul-e Boqraat), Summary of Experiences (Kholasa-tut Tajarob), Description of Signs and Symptoms (Sharh-e Asbab va Alamat), and Aghili’s Treatments (Mo’alejat-e Aghili). Moreover, Scopus, PubMed, Web of Science, SienceDirect, and Google Scholar databases were queried with the keywords ‘mizaj’, ‘temperament’, ‘uterus’, ‘uterine’, ‘cold/hot or warm’, and ‘dry/wet’, while Persian databases of Magiran, Iran Medex, and SID were searched using keywords ‘mizaj’, ‘rahem’, ‘garm’, ‘sard’, ‘tar’, and ‘khoshk’ to extract signs and symptoms associated with uterine temperament and dystemperament. Results: Five indicators were specified in the articles and PM sources for determining the temperament and dystemperament of the uterus as follows: 1) Menstrual blood characteristics (including amount, temperature, color, odor, flow rate, and consistency); 2) Uterine discharge characteristics (including amount, consistency, color, odor, and accompanied symptoms, especially burning and itching); 3) Fertility and pregnancy status (including infertility, abortion, etc.); 4. Other characteristics of the female reproductive system (including pubic hair, menarche age, sexual desire and quality of intercourse, and vaginal and cervical condition on vaginal examination); and 5) General symptoms (including the ten PM indicators of whole-body Mizaj). Conclusions: PM sources have described the characteristics of uterine temperament and dystemperament in detail. These indices can be used for more effective diagnosis, treatment, and even prevention of gynecological diseases, as well as designing and validation of standard tools for determining uterine temperament and dystempe
目的：根据波斯医学（PM），子宫是女性的重要器官，负责净化血液和滋养胎儿。每个器官都有一种特定的气质，不同于基于PM的全身气质。当身体或器官Mizaj（波斯语中气质的意思）偏离正常状态，导致功能障碍时，就会出现气质障碍。PM和传统医学中描述的许多妇科疾病，包括不孕不育、反复流产、月经过少/闭经、月经过多、阴道炎、宫颈炎、尿失禁和盆腔疼痛，都被认为与子宫脾气不好有关。因此，正确治疗此类疾病需要精确诊断和治疗子宫脾气异常。因此，本综述研究旨在从PM文本和其他相关文章中收集和分类这些症状。方法：在本研究中，我们回顾了10篇著名的PM参考文献，包括《医学经典》（Al-Qanun Fi Al-Teb）、《大Panacea》（Exir-e A'zam）、《哈拉兹姆沙之宝》（Zakhireh Kharazmshahi）、《医学艺术的完美之书》（Kamel Al-Sana'A Al-Tebbiya）、《阿克巴里医学》（Tebb-e-Akbari）、《加尔希医学经典评论》（Sharh-e Qarshi bar Qanun），对希波克拉底格言的评论（Sharh-e Fosul-e Boqraat）、经验总结（Kholasa tut Tajarob）、体征和症状描述（Sharh-e-Asbab va Alamat）以及阿吉利的治疗方法（Mo'alejat-e Aghili）。此外，Scopus、PubMed、Web of Science、SienceDirect和Google Scholar数据库使用关键字“mizaj”、“气质”、“子宫”、“宫”、“冷/热或暖”和“干/湿”进行查询，而Magiran、Iran Medex和SID的波斯数据库则使用关键字“mizaj”，和“khoshk”提取与子宫气质和气质障碍相关的体征和症状。结果：文章和PM来源中规定了五个指标来确定子宫的气质和不气质，如下：1）月经血液特征（包括量、温度、颜色、气味、流速和稠度）；2） 子宫分泌物特征（包括数量、稠度、颜色、气味以及伴有的症状，尤其是灼热和瘙痒）；3） 生育能力和妊娠状况（包括不孕不育、流产等）；4.女性生殖系统的其他特征（包括阴毛、初潮年龄、性欲和性交质量，以及阴道检查中的阴道和宫颈状况）；和5）一般症状（包括全身Mizaj的10个PM指标）。结论：PM资料详细描述了子宫气质和气质障碍的特点。这些指标可用于更有效的妇科疾病诊断、治疗甚至预防，以及设计和验证用于确定子宫气质和气质障碍的标准工具。
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