Trends in Analytical Chemistry最新文献

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Biosensor technologies for natural medicine discovery: current advances, challenges, and future directions 用于天然药物发现的生物传感器技术：当前进展、挑战和未来方向

IF 12 1区化学 Q1 CHEMISTRY, ANALYTICAL

Trends in Analytical Chemistry

Pub Date : 2025-10-15 DOI: 10.1016/j.trac.2025.118498

Lu Shen , Fei Gao , Jing Wei , Die Gao , Dalian Qin , Xiao Wang , Yujiao Ji , Xiaobing An , Xiaogang Zhou , Jianming Wu , Jianing Mi , Lixian Li , Bin Huang , Anguo Wu

The growing interest in natural medicines highlights the need for rapid and accurate analytical methods to identify bioactive compounds. Conventional techniques such as high-performance liquid chromatography, mass spectrometry, and nuclear magnetic resonance are precise but limited by time, cost, and complexity, restricting their use in high-throughput screening. Biosensor technologies offer real-time, label-free, and highly sensitive detection, emerging as powerful alternatives. This review summarizes recent advances in optical, electrochemical, and microfluidic-integrated biosensors, along with their applications in screening antiviral, anti-inflammatory, anticancer, neuroprotective, and nucleic acid-targeting compounds. Key challenges, including matrix interference, specificity, sensitivity, throughput, and standardization, are discussed. Future directions focus on enhancing sensor performance through surface modifications, improved sample processing, multiplexing, and artificial intelligence-assisted data integration. These strategies aim to fully realize the potential of biosensors in accelerating the discovery of novel and effective therapeutic agents from natural sources.

人们对天然药物的兴趣日益浓厚，因此需要快速准确的分析方法来鉴定生物活性化合物。传统的技术，如高效液相色谱、质谱和核磁共振是精确的，但受时间、成本和复杂性的限制，限制了它们在高通量筛选中的应用。生物传感器技术提供实时、无标签和高灵敏度的检测，正在成为强大的替代方案。本文综述了光学、电化学和微流体集成生物传感器的最新进展，以及它们在筛选抗病毒、抗炎、抗癌、神经保护和核酸靶向化合物方面的应用。关键的挑战，包括基质干扰，特异性，灵敏度，吞吐量和标准化，进行了讨论。未来的方向是通过表面修饰、改进样品处理、多路复用和人工智能辅助数据集成来提高传感器性能。这些策略旨在充分发挥生物传感器的潜力，加速从天然来源发现新的有效治疗剂。

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引用次数: 0

Towards an efficient and sustainable technique: A review on the preparation and synthesis of sorbents for in-tube solid-phase microextraction 迈向高效可持续技术：管内固相微萃取吸附剂的制备与合成综述

IF 12 1区化学 Q1 CHEMISTRY, ANALYTICAL

Trends in Analytical Chemistry

Pub Date : 2025-10-15 DOI: 10.1016/j.trac.2025.118501

Enrico Taglioni, Chiara Cavaliere, Andrea Cerrato, Aldo Laganà, Carmela Maria Montone, Anna Laura Capriotti

Microextraction techniques are increasingly valued for their low sample requirements and consistency with green chemistry principles. Over time, several formats have been developed, enabling faster and automated analytical workflows. Among them, in tube-solid phase microextraction (IT-SPME) stands out as a powerful sample preparation technique, offering direct online coupling with chromatographic systems, automation, and reduced solvent use. This review critically examines the main strategies for IT-SPME sorbent fabrication, including fiber-filled, monolithic, coated, and particle-packed capillaries. Emphasis is set on the underlying chemistry of sorbent synthesis, radical polymerization, sol-gel processes, hydrothermal reactions, and click chemistry, while assessing efficiency, scalability, and environmental impact. Challenges such as complex syntheses, hazardous reagents, and artisanal protocols are discussed. The review offers the analytical community a practical guide to reproducible and sustainable strategies for developing IT-SPME devices that are well-suited to automated workflows.

微萃取技术因其低样品要求和符合绿色化学原理而越来越受到重视。随着时间的推移，已经开发了几种格式，支持更快和自动化的分析工作流。其中，管内固相微萃取（IT-SPME）作为一种强大的样品制备技术脱颖而出，提供了与色谱系统的直接在线耦合，自动化和减少溶剂使用。这篇综述严格审查了IT-SPME吸附剂制造的主要策略，包括纤维填充、整体、涂层和颗粒填充毛细血管。重点放在吸附剂合成，自由基聚合，溶胶-凝胶过程，水热反应和点击化学的基础化学，同时评估效率，可扩展性和环境影响。挑战，如复杂的合成，危险的试剂，和手工协议进行了讨论。该综述为分析界提供了可重复和可持续的策略，以开发非常适合自动化工作流程的IT-SPME设备的实用指南。

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引用次数: 0

Advances in skin-on-a-chip technology: Functional innovations and translational challenges 芯片上皮肤技术的进展：功能创新和转化挑战

IF 12 1区化学 Q1 CHEMISTRY, ANALYTICAL

Trends in Analytical Chemistry

Pub Date : 2025-10-14 DOI: 10.1016/j.trac.2025.118500

Jingfan Zhang , Qingwen Zhang , Xiang Fang , Guangsheng Guo , Xiayan Wang

As the body's largest organ, skin plays essential roles in maintaining physiological homeostasis. While indispensable for research and product development, conventional in vitro skin models cannot fully recapitulate recapitulate three-dimensional microenvironments, dynamic perfusion, and multicellular interactions, impeding accurate modeling of barrier functions, immune regulation, and drug permeation. Emerging skin-on-a-chip (SoC) technologies overcome these constraints through innovative microfluidic architectures, controlled fluidics, and multicellular integration, enabling the reconstruction of hierarchical skin structures. However, challenges remain, including material adsorption, absence of appendages, incomplete functional characterization, and a lack of standardized protocols. This review systematically evaluates technological advancements and translational barriers in SoC development. We first outline the native skin's layered architecture and cellular heterogeneity to reveal current modeling limitations. Subsequent analysis focuses on key innovations: biomimetic structural design, advanced biomaterials, biomechanical simulation, and microfluidic regulation. We further examine characterization methodologies and application scenarios. By integrating current findings, we demonstrate the potential of SoC platforms to revolutionize skin pathology research via omics-enabled spatiotemporal profiling and to serve as standardized platforms for product testing and health monitoring. Realizing this potential will require interdisciplinary collaboration to overcome critical bottlenecks in appendage integration, high-throughput detection, and manufacturing standardization—advances poised to transform conventional evaluation paradigms.

皮肤作为人体最大的器官，在维持生理稳态中起着至关重要的作用。虽然对于研究和产品开发不可或缺，但传统的体外皮肤模型不能完全再现三维微环境、动态灌注和多细胞相互作用，阻碍了屏障功能、免疫调节和药物渗透的准确建模。新兴的片上皮肤（SoC）技术通过创新的微流体架构、控制流体和多细胞集成克服了这些限制，使分层皮肤结构得以重建。然而，挑战仍然存在，包括材料吸附，缺乏附件，不完整的功能表征，以及缺乏标准化的方案。本文系统地评估了SoC开发中的技术进步和转化障碍。我们首先概述了原生皮肤的分层结构和细胞异质性，以揭示当前建模的局限性。随后的分析集中在关键的创新：仿生结构设计，先进的生物材料，生物力学模拟和微流体调节。我们进一步研究表征方法和应用场景。通过整合目前的研究结果，我们展示了SoC平台的潜力，通过组学支持的时空分析来彻底改变皮肤病理学研究，并作为产品测试和健康监测的标准化平台。实现这一潜力需要跨学科合作，以克服附件集成、高通量检测和制造标准化方面的关键瓶颈——这些进步将改变传统的评估范式。

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引用次数: 0

Aggregation-induced emission-enhanced point-of-care testing method for food safety and environmental monitoring 用于食品安全和环境监测的聚合诱导排放增强护理点检测方法

IF 12 1区化学 Q1 CHEMISTRY, ANALYTICAL

Trends in Analytical Chemistry

Pub Date : 2025-10-14 DOI: 10.1016/j.trac.2025.118493

Liyuan Wang , Xiaofeng Hu , Shenling Wang , Wenqin Wu , Kaiming Wu , Li Yu , Peiwu Li , Zhaowei Zhang

As urgent public health challenges, food safety and environmental monitoring have drawn growing attention. Point-of-care testing (POCT) represents a cost-effective, rapid, accurate, and convenient means of quantitative detection. The unique fluorescent phenomenon induced by aggregation-induced emission (AIE) holds substantial promise for enhancing POCT capabilities in detecting food and environmental contaminants. This work reviewed recent advancements and challenges in developing the AIE-enhanced POCT method. Firstly, the principles and advantages of the AIE-powered POCT method were discussed. Subsequently, the applications of the AIE-enhanced POCT method in detecting environmental and food contamination were summarized. Finally, the potential commercialization and challenges associated with AIE-powered POCT methods are discussed. This review refreshes the perspective to develop portable, cost-effective sensing solutions and encourages collaborative efforts to advance sensing technology.

作为紧迫的公共卫生挑战，食品安全和环境监测日益引起人们的关注。即时检测（POCT）是一种具有成本效益、快速、准确和方便的定量检测手段。由聚集诱导发射（AIE）引起的独特荧光现象为增强POCT检测食品和环境污染物的能力提供了巨大的希望。本文综述了aie增强POCT方法的最新进展和挑战。首先，讨论了基于ae的POCT方法的原理和优点。随后，总结了aie增强POCT方法在环境和食品污染检测中的应用。最后，讨论了与ai驱动的POCT方法相关的潜在商业化和挑战。这篇综述刷新了开发便携式、具有成本效益的传感解决方案的前景，并鼓励合作努力推进传感技术。

引用次数: 0

Molecularly imprinted optical sensors for disease biomarker analysis: current progress and future trends 用于疾病生物标志物分析的分子印迹光学传感器：目前进展和未来趋势

IF 12 1区化学 Q1 CHEMISTRY, ANALYTICAL

Trends in Analytical Chemistry

Pub Date : 2025-10-14 DOI: 10.1016/j.trac.2025.118499

Ganping Cai , Huifeng Zhang , Ran Zhu , Yongcun Zou , Qiong Jia

The deep integration of molecularly imprinted polymers (MIPs) with optical sensing technologies demonstrates promising potential in the analysis of disease biomarkers. By incorporating multimodal optical sensing approaches including fluorescence (FL), surface-enhanced Raman scattering (SERS), surface plasmon resonance (SPR), colorimetry (CM), optics fiber (OF), photonic crystals (PC), and electrochemiluminescence (ECL), MIP-based optical sensors have enabled precise identification of disease biomarkers (e.g., proteins, extracellular vesicles, small molecules, and microorganisms). This review systematically summarizes recent advances in MIPs-based optical sensors, focusing on their design principles, construction strategies, and applications in diagnosing cancers, cardiovascular diseases, neurodegenerative diseases, and bacterial infectious diseases, while also discussing future development trends.

分子印迹聚合物（MIPs）与光学传感技术的深度融合在疾病生物标志物分析中显示出巨大的潜力。通过结合多模态光学传感方法，包括荧光（FL）、表面增强拉曼散射（SERS）、表面等离子体共振（SPR）、比色法（CM）、光纤（OF）、光子晶体（PC）和电化学发光（ECL），基于mip的光学传感器能够精确识别疾病生物标志物（例如蛋白质、细胞外囊泡、小分子和微生物）。本文系统地综述了基于mips的光学传感器的设计原理、构建策略及其在癌症、心血管疾病、神经退行性疾病和细菌感染性疾病诊断中的应用，并对未来的发展趋势进行了讨论。

引用次数: 0

CRISPR/Cas system-based strategies for pathogenic bacteria detection 基于CRISPR/Cas系统的病原菌检测策略

IF 12 1区化学 Q1 CHEMISTRY, ANALYTICAL

Trends in Analytical Chemistry

Pub Date : 2025-10-13 DOI: 10.1016/j.trac.2025.118497

Yijia Zheng, Xinyi Sun, Xijiao Li, Jing Lei, Erqun Song

The effective detection of pathogenic bacteria is crucial for safeguarding human health, as these pathogenic bacteria cause a wide range of diseases. Conventional detection methods are often limited by their specificity, sensitivity, and speed, hindering timely detection of pathogenic bacteria. In recent years, the CRISPR/Cas system-comprising clustered regularly interspaced short palindromic repeats (CRISPR) and its associated protein (Cas) - has emerged as a powerful platform for developing such detection methods for pathogenic bacteria, owing to its high specificity in recognizing and cleaving nucleic acid sequences. This manuscript begins with a brief introduction to the origin, classification, working principle, and characteristics of the CRISPR/Cas system. It then comprehensively reviews the recent advances of CRISPR/Cas-based methods integrated with various signal output modalities for the detection of pathogenic bacteria. Finally, the advantages, current limitations, and future prospects of CRISPR/Cas system-based methods for pathogenic bacteria assay are discussed.

病原菌的有效检测对保障人类健康至关重要，因为这些病原菌引起的疾病范围广泛。传统的检测方法往往受其特异性、敏感性和速度的限制，阻碍了病原菌的及时检测。近年来，CRISPR/Cas系统——包括簇状规则间隔短回文重复序列（CRISPR）及其相关蛋白（Cas）——由于其识别和切割核酸序列的高特异性，已成为开发致病菌检测方法的强大平台。本文首先简要介绍了CRISPR/Cas系统的起源、分类、工作原理和特点。然后全面回顾了基于CRISPR/ cas的方法与各种信号输出方式相结合用于病原菌检测的最新进展。最后，讨论了基于CRISPR/Cas系统的病原菌检测方法的优势、局限性及未来展望。

引用次数: 0

Advanced nucleic acid probes for enhanced CRISPR diagnostics 先进的核酸探针增强CRISPR诊断

IF 12 1区化学 Q1 CHEMISTRY, ANALYTICAL

Trends in Analytical Chemistry

Pub Date : 2025-10-13 DOI: 10.1016/j.trac.2025.118496

Muyang Liu , Bingxin Zheng , Mengqiu Xiong , Bangshun He , Chunlan Qiu , Tao Li , Ying Li

With the continuous advancement of molecular diagnostic technologies, CRISPR-based nucleic acid detection platforms have emerged as powerful tools due to their programmability, sensitivity, and operational simplicity. While conventional ssDNA and ssRNA probes are widely used, recent studies have demonstrated that advanced nucleic acid probes, such as hairpins, G-triplexes, and G-quadruplexes, can further enhance CRISPR detection by improving signal stability, mismatch discrimination, and amplification-free sensitivity. However, their role has not been comprehensively reviewed. This article provides a systematic summary of these high-order probes, classifying them by structure and signal mechanism, and analyzing their contributions to Cas12/Cas13-based systems. We also highlight how these innovations address key challenges such as low trans-cleavage turnover and background noise, enabling CRISPR diagnostics to achieve greater robustness, specificity, and real-world clinical potential. By integrating molecular design with diagnostic function, these probes represent a promising direction for advancing CRISPR-based detection toward next-generation applications in point-of-care testing.

随着分子诊断技术的不断进步，基于crispr的核酸检测平台以其可编程性、灵敏性、操作简便等优点成为强大的检测工具。虽然传统的ssDNA和ssRNA探针被广泛使用，但最近的研究表明，先进的核酸探针，如发夹、g -三丛和g -四丛，可以通过提高信号稳定性、错配辨别和无扩增灵敏度来进一步增强CRISPR检测。然而，它们的作用尚未得到全面审查。本文对这些高阶探针进行了系统的总结，根据结构和信号机制对它们进行了分类，并分析了它们对基于Cas12/ cas13的系统的贡献。我们还强调了这些创新如何解决诸如低反式切割周转率和背景噪声等关键挑战，使CRISPR诊断获得更大的稳健性、特异性和现实世界的临床潜力。通过将分子设计与诊断功能相结合，这些探针代表了将基于crispr的检测推向下一代护理点检测应用的有希望的方向。

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引用次数: 0

Strategies for enhancing the signal-to-noise ratio in optical lateral flow immunoassay: A review 提高光学侧流免疫分析信噪比的策略综述

IF 12 1区化学 Q1 CHEMISTRY, ANALYTICAL

Trends in Analytical Chemistry

Pub Date : 2025-10-12 DOI: 10.1016/j.trac.2025.118494

Jingrui Yuan , Liang Huang , Jing Wang

Enhancing the sensitivity of lateral flow immunoassays (LFIA) is pivotal for advancing point-of-care diagnosis, primarily by improving the signal to noise (S/N) ratio. This review comprehensively summarizes recent strategies aimed at both amplifying signals and suppressing background noise. Signal enhancement approaches encompass sample amplification (target pre-amplification and enrichment), optimization of immune recognition efficiency (through kinetic regulation and increased reaction probability), and diverse signal amplification techniques (e.g., assembly-based amplification, metal-enhanced fluorescence, advanced detection modalities). Methods to reduce background interference involve low-excitation background strategies (chemiluminescence, magnetically modulated luminescence), low-optical detection background techniques (e.g., time-gated noise suppression, wavelength-selective noise reduction, and scattered light detection). The review also traces the evolution of these methodologies, offering context for their development and guiding researchers in navigating the LFIA platform. Overall, this work serves as an essential resource for enhancing the S/N ratio in LFIA and provides valuable perspectives for the design of next-generation LFIA systems.

通过提高信噪比（S/N），提高侧流免疫测定（LFIA）的敏感性对推进护理点诊断至关重要。这篇综述全面总结了最近旨在放大信号和抑制背景噪声的策略。信号增强方法包括样品扩增（靶标预扩增和富集）、优化免疫识别效率（通过动力学调节和增加反应概率）以及多种信号放大技术（如基于组装的扩增、金属增强荧光、先进的检测方式）。减少背景干扰的方法包括低激发背景策略（化学发光、磁调制发光）、低光学检测背景技术（例如，时间门控噪声抑制、波长选择性噪声抑制和散射光检测）。该综述还追溯了这些方法的演变，为它们的发展提供了背景，并指导研究人员导航LFIA平台。总的来说，这项工作是提高LFIA信噪比的重要资源，并为下一代LFIA系统的设计提供了有价值的视角。

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Recent advances in nucleic acid-based probes for organelle-specific imaging 基于核酸的细胞器特异性成像探针的最新进展

IF 12 1区化学 Q1 CHEMISTRY, ANALYTICAL

Trends in Analytical Chemistry

Pub Date : 2025-10-12 DOI: 10.1016/j.trac.2025.118495

Ximei Zhang, Guangmin Zhang, Lanzhen He, Lanlan Chen, Huanghao Yang

Recent advancements in nucleic acid-based nanoprobes have revolutionized targeted subcellular imaging, offering unprecedented precision and versatility in visualizing cellular components. This review highlights the design and application of these probes, which leverage their high specificity to bind target molecules, enabling accurate imaging and dynamic tracking of organelles such as mitochondria, lysosomes, and the endoplasmic reticulum. By integrating signal amplification and fluorescence modules, these probes significantly enhance imaging sensitivity, making them invaluable tools in disease diagnosis, drug development, and biomedical research. Furthermore, their cross-disciplinary potential extends to gene expression regulation, disease mechanism elucidation, and personalized therapy. This comprehensive overview underscores the transformative impact of nucleic acid-based probes in advancing analytical chemistry and life sciences, paving the way for future innovations in subcellular imaging and therapeutic applications.

基于核酸的纳米探针的最新进展彻底改变了靶向亚细胞成像，在可视化细胞成分方面提供了前所未有的精度和多功能性。这篇综述强调了这些探针的设计和应用，它们利用它们的高特异性来结合靶分子，实现细胞器如线粒体、溶酶体和内质网的精确成像和动态跟踪。通过集成信号放大和荧光模块，这些探针显着提高了成像灵敏度，使其成为疾病诊断，药物开发和生物医学研究的宝贵工具。此外，它们的跨学科潜力扩展到基因表达调控，疾病机制阐明和个性化治疗。这一全面的概述强调了基于核酸的探针在推进分析化学和生命科学方面的变革性影响，为亚细胞成像和治疗应用的未来创新铺平了道路。

引用次数: 0

Recent advances in point-of-care testing devices for transfusion medicine 输血医学即时检测设备的最新进展

IF 12 1区化学 Q1 CHEMISTRY, ANALYTICAL

Trends in Analytical Chemistry

Pub Date : 2025-10-11 DOI: 10.1016/j.trac.2025.118490

Rijo Rajeev , Paresh Mohanty , Suvro Sankha Datta , Parikshit Moitra

A major concern for patients with blood-based disorders is their requirement for frequent blood transfusions, which is one of the primary causes of increased morbidity and mortality among these groups, especially in low- and middle-income countries. Various efforts have been made to extend blood grouping, genotyping, and compatibility testing to improve blood transfusion practices, especially for patients affected with β-thalassemia, sickle cell disease, aplastic anemia, and cancer. Precise blood typing will assist in optimizing donor-recipient compatibility testing, thereby reducing alloimmunization risk and post-transfusion complications. Different Point-of-Care-Testing (POCT) devices have been transformed into trailblazers, opening various avenues in transfusion medicine practices as they automate, reduce the number of steps, and have a profound ability to reproduce results rapidly with high economic benefits that contribute significantly to the healthcare sectors. The current state-of-the-art POCTs include wearables, flexible, paper-based, chip-based, printed-type, microfluidics, and lateral flow-based assays, as discussed in this review article. In addition, exciting insights into various other applications of POCTs in hemoglobin and hematocrit measurement, identifying storage lesions, and monitoring of bacterial contamination prior to platelet transfusion among others have been comprehensively discussed. This review thus intends to provide a critical perspective focusing on the future direction of POCTs for the efficient practice of transfusion medicine.

血液病患者的一个主要关切是他们需要经常输血，这是这些群体中发病率和死亡率增加的主要原因之一，特别是在低收入和中等收入国家。已作出各种努力，扩大血型、基因分型和相容性测试，以改善输血做法，特别是对患有β-地中海贫血、镰状细胞病、再生障碍性贫血和癌症的患者。精确的血型将有助于优化供体-受体相容性测试，从而减少同种异体免疫风险和输血后并发症。不同的护理点测试（POCT）设备已经转变为开拓者，在输血医学实践中开辟了各种途径，因为它们自动化，减少了步骤数量，并且具有快速重现结果的深刻能力，具有高经济效益，对医疗保健部门做出了重大贡献。如本文所述，目前最先进的poct包括可穿戴、柔性、纸质、芯片、印刷型、微流体和基于横向流动的检测。此外，对POCTs在血红蛋白和红细胞压积测量、识别储存病变和血小板输注前细菌污染监测等方面的各种其他应用的令人兴奋的见解也进行了全面讨论。因此，本综述旨在提供一个关键的观点，重点关注poct的未来方向，为输血医学的有效实践。

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