Trends in Analytical Chemistry最新文献

DNA nanotechnology is driven by the precision and programmable properties of DNA, which serves as a reliable template to guide the self-assembly of various materials, such as metal nanoparticles and polymers. By integrating DNA nanotechnology into the synthesis of plasmonic nanomaterials, unprecedented precision in structural control is achieved, facilitating the fabrication of pre-designed nanostructures with modulated optical functionalities. In this comprehensive review, we explore various synthesis techniques (e.g., DNA linker-based assembly, DNA origami-based organization, and DNA-mediated growth), focusing on advanced DNA-assembled plasmonic nanoarchitecture (DAPNA). The article highlights the applications of DAPNA in localized surface plasmon resonance (LSPR) and surface-enhanced Raman scattering (SERS) based biosensors for diagnostic purposes. The review not only addresses the limitations and solutions in DAPNA manufacturing technology but also explores potential applications in biosensing, tissue engineering, gene editing, immunotherapy, and other emerging fields.

Rapid detection of foodborne pathogens is crucial for protecting food safety and minimizing economic losses. Optical biosensors recognize and quantify pathogenic bacteria through various optical signals, and have shown their advantages of fast response, high sensitivity and easy integration. This review described the basic principles of optical biosensors and discussed the essential considerations in sample collection, pathogen isolation and pathogen detection on the applications of optical biosensors for foodborne pathogen detection. Besides, recent advances of the optical biosensors including SPR biosensors, interference biosensors, SERS biosensors, fluorescence biosensors, chemiluminescence biosensors, and colorimetry biosensors reported in the past three years were reviewed. More importantly, the state-of-the-art research and development trends of optical biosensors for the detection of foodborne pathogens were summarized. This review might provide a comprehensive reference for the development of optical biosensors to detect foodborne pathogens.

Emerging organic pollutants (EOPs) are a class of hazardous and poisonous organic substances with high biotoxicity, bioaccumulation and long-term residual. They can be enriched in organisms, leading to great risks to ecosystem and human health. Therefore, it is crucial to establish sensitive and reliable analytical methods to identify and detect EOPs in biological samples. Solid phase microextraction (SPME) has been well explored as an effective, environmental-friendly and automated sample preparation technique for bioanalysis. The development of high-efficiency SPME coatings, convenient sampling approaches and practical analytical instruments significantly promotes the rapid screening and accurate determination of EOPs. This review summarizes the SPME application of diverse EOPs in complex biological matrices, and also presents in vitro and in vivo SPME for bioanalysis. In addition, the promising biocompatible coatings and recently used analytical instruments are reviewed in this work, offering new perspectives for the innovation on SPME-based bioanalytical methods.

Anthracycline antibiotics (doxorubicin, daunorubicin, mitoxantrone, idarubicin, epirubicin, ect.) are widely used in chemotherapy of various diseases, effectively destroying cancer cells, but also affecting healthy cells and organs. Clinical monitoring of anthracycline antibiotics in biofluids is critical for optimization of therapy and minimization of side effects, since toxicity increases at too high concentrations, while desired chemotherapy effects may be reduced or lost at too low concentrations. The development of approaches based on the luminescence quenching of nanosensors makes it possible to develop simple fast inexpensive methods for monitoring anthracycline antibiotics on the basis of their intrinsic properties. The advantages of turn-off luminescence nanosensors are discussed, a closer look is given to the applications for anthracycline antibiotics analysis in biofluids with focus on their limitations.

In the field of cancer detection, the development of affordable, quick, and user-friendly sensors capable of detecting various cancer biomarkers, including those for lung cancer (LC), holds utmost significance. Sensors are expected to play a crucial role in the early-stage diagnosis of various diseases. Among the range of options, sensors emerge as particularly appealing for the diagnosis of various diseases, owing to their cost-effectiveness, simplicity, and promising analytical performance. There is growing interest in the application of molecularly imprinted polymers (MIPs) as promising recognition elements in gas sensors. MIPs, as a leading technology for sensing analytes where no suitable bioreceptor exists, are commonly used in artificial sensing that can be applied in key fields like early disease diagnostics, based on the detection of volatile biomarkers. There is an extensive demand for early, non-invasive detection of various diseases and for the self-monitoring of health conditions. Detection of biomarkers in point-of-care mode remains challenging and is limited by various factors. Hence, breath analysis has received enormous attention in healthcare due to its relatively low cost, non-invasive sampling method, and rapid detection capabilities. The latest developments in MIP-based sensors and their utility in disease diagnosis through the detection of volatile biomarkers are comprehensively and critically evaluated in this review. Furthermore, the challenges and perspectives of MIP-based sensors are elaborated upon, with a view towards introduction to the market and successful commercialization.

Optical probe-based biosensors (OPBs) have proven instrumental in human health monitoring. The growing portability and miniaturization of OPBs have rendered them cost-effective and user-friendly, presenting great potential for the advancement of point-of-care testing (POCT) platforms. To further expand their applications, ongoing efforts have been made to enhance their detection performance, particularly focusing on sensitivity. This review provides a comprehensive overview on existing OPBs in POCT and summarizes their underlying detection principles. Additionally, this review highlights four strategies and approaches for enhancing the performance of OPBs: optimizing target-capturing capability, improving the conversion efficiency of biological signals to optical signals, enhancing the separation ability of signal separation system, and optimizing the signal recognition ability of the reader. By focusing on these strategies, this review explores the potential of optimized OPBs in expanding their applications across various human health-related domains.

Endocrine-disrupting compounds (EDCs) are a group of chemicals that interfere with the endocrine system, leading to adverse effects on human health and the environment. Increasing concerns over the EDCs presence in various environmental compartments has driven the search for greener extraction materials. Recently, the use of polymers of natural origin (biopolymers) has been demonstrated to be an effective and promising research direction due to their undeniable advantages over synthetic polymers. In this review, strategies for cellulose, chitin, and chitosan functionalization and their applicability for numerous microextraction techniques have been widely discussed. Following the trend related to the reuse of waste, various agricultural wastes that were employed for the isolation and enrichment of EDCs are described. The benefits and limitations of using natural sorbents have been highlighted.

For years, scientists have been trying to develop sustainable, environmentally friendly methods of extraction and determination of trace environmental pollutants that take into account the principles of Green Analytical Chemistry (GAC). It is important to save energy and time, minimize and automate individual stages of sample preparation for determination, and significantly reduce or completely eliminate harmful wastes and solvents from the analytical procedure. Therefore, there is continuous development and increase in the popularity of solid-phase extraction (SPE) technique and its derivative techniques in the “micro” form, such as dispersive micro-solid-phase extraction (d-μSPE), magnetic dispersive micro-solid-phase extraction (M-d-μSPE), bar adsorption microextraction (BAμE), rotating disk sorption extraction (RDSE), fabric phase sorption extraction (FPSE), solid phase microextraction (SPME), thin film microextraction (TFME). An important and noticeable trend in recent years has been the search for efficient sorbents based on natural compounds.

This review discusses the possibilities of using natural products (cork, cotton, pollen, kapok, bamboo, sponge, algae, various seeds) and biowastes (coffee residues, bracts and peels of various fruits) as sorbents in the above-mentioned solid-phase techniques.

These natural materials have fibrous or porous structure, which facilitates sorption of analytes. They also have functional groups necessary for the extraction of selected analytes or may be easily modified to contain such groups. Many of 106 articles described in this review show that sorbents based on natural materials are as good as commercially available synthesized sorbents while being more friendly to the environment.

In-vitro diagnostics (IVD) has become an in-demand area with a high-achievement transformation due to the expansion of new technologies. Technology has grown from time-consuming laboratory techniques to modern automated immunoassays and progressed with real-time sensing through the emergence of biosensor devices. Plasmonic biosensors are one of the most employed optical biosensors and stand out for their versatility and wide range of applications in diagnostic tests. One of the clinical diagnostics areas of growing interest is autoimmune diseases (AD), which have been increasingly prevalent worldwide. There is a rising demand for reliable and early AD diagnosis tools. This review highlights the main challenges for early AD diagnostics and the advanced plasmonic biosensors that have been exploited for AD biomarker detection, including brief descriptions of their analytical performance. Finally, the prospects and trends in this area are outlined, thinking about developing multiplexed platforms capable of addressing the multianalyte nature of one disease.

Paper-based analytical devices (PADs) enable the affordable, easy, rapid, and reliable detection of a range of analytes at the point-of-care. Cellulose is a versatile substrate for antibody attachment. However, how immunoreagents are immobilized onto cellulose plays an important role in assay performance. To provide an overview of the different antibody immobilization strategies used for developing PADs, we here critically review the existing literature from the last decade (2013–2023). First, we introduce cellulose as a substrate and summarize the different fabrication techniques for developing PADs. Thereafter, we delve into how antibodies are immobilized onto cellulose passively, covalently, or by affinity linkage. Affinity-based immobilization is further explored in a case study focused on using recombinant bifunctional proteins with cellulose-binding modules to anchor immunoreagents to cellulose. The final section discusses the validation and sustainable development of PADs addressing the existing and future challenges associated with developing such devices.