Pub Date : 2026-01-14DOI: 10.1016/j.trac.2026.118673
Shuai Zhang , Kavirajaa Pandian Sambasevam , Faizah Muhammad Yunus , Waleed Alahmad , Nor Kartini Binti Abu Bakar , Muggundha Raoov
This review is the first to provide a systematic integration of deep eutectic solvents (DESs) into multi-template molecular imprinting, establishing DESs-based multi-template molecularly imprinted polymers (DESs-mt-MIPs) as an emerging class of green affinity materials. The review systematically covers the fundamental principles of molecular imprinting, emphasizing the advantages and mechanisms of DESs-based multiple-template imprinting over traditional MIPs approaches. This review also investigates the pivotal functions of DESs as both functional monomers and porogens in the fabrication of mt-MIPs, demonstrating their ability to enhance the polymers' selectivity, adsorption capacity, and environmental friendliness. Furthermore, the review presents various innovative synthesis techniques, characterizes the structural and functional properties of DESs-mt-MIPs, and discusses their applications in environmental pollutant removal, biomedical fields, and food safety. Despite the promising potential, current challenges, including the type of DESs that can be used in MIPs synthesis, the combination of multiple templates, stability, reproducibility, and large-scale production, are highlighted, and future research directions are proposed to address these issues and explore mt-MIPs in DESs further.
{"title":"Advancements of multiple template-based molecularly imprinted polymers (mt-MIPs) in deep eutectic solvents (DESs): Towards greener imprinting approaches","authors":"Shuai Zhang , Kavirajaa Pandian Sambasevam , Faizah Muhammad Yunus , Waleed Alahmad , Nor Kartini Binti Abu Bakar , Muggundha Raoov","doi":"10.1016/j.trac.2026.118673","DOIUrl":"10.1016/j.trac.2026.118673","url":null,"abstract":"<div><div>This review is the first to provide a systematic integration of deep eutectic solvents (DESs) into multi-template molecular imprinting, establishing DESs-based multi-template molecularly imprinted polymers (DESs-mt-MIPs) as an emerging class of green affinity materials. The review systematically covers the fundamental principles of molecular imprinting, emphasizing the advantages and mechanisms of DESs-based multiple-template imprinting over traditional MIPs approaches. This review also investigates the pivotal functions of DESs as both functional monomers and porogens in the fabrication of mt-MIPs, demonstrating their ability to enhance the polymers' selectivity, adsorption capacity, and environmental friendliness. Furthermore, the review presents various innovative synthesis techniques, characterizes the structural and functional properties of DESs-mt-MIPs, and discusses their applications in environmental pollutant removal, biomedical fields, and food safety. Despite the promising potential, current challenges, including the type of DESs that can be used in MIPs synthesis, the combination of multiple templates, stability, reproducibility, and large-scale production, are highlighted, and future research directions are proposed to address these issues and explore mt-MIPs in DESs further.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"196 ","pages":"Article 118673"},"PeriodicalIF":12.0,"publicationDate":"2026-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146034661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-13DOI: 10.1016/j.trac.2026.118671
Mei Du , Zecong Yu , Rongxin Lin , Mengfan Niu , Yue Gao , Lei Wang , Xianghao Zha , Maoyong Song , Guibin Jiang
Phenolic compounds (PCs) are significant environmental contaminants that pose a serious threat to human health and ecological systems. Developing rapid and accurate monitoring of residual PCs concentrations is essential for effective environmental monitoring. Nanozymes, which combine enzyme-mimicking catalytic activities with the unique physicochemical properties of nanomaterials, have gained significant attention in biosensing. However, a systematic review focused specifically on nanozyme-based biosensors for PCs detection remains lacking. This review systematically summarizes the types and catalytic mechanisms of nanozymes relevant to PCs detection and comprehensively discusses the design principles of biosensors for PCs contaminants. Furthermore, the role of nanozymes in signal transduction and amplification is thoroughly explored across both single and dual detection modalities. Afterwards, the latest nanozyme-integrated sensing platforms are also discussed. Finally, current challenges and future perspectives for intelligent nanozyme-based biosensors in environmental contaminants monitoring are outlined, aiming to promote broader application in related fields.
{"title":"Recent advances in nanozyme-based biosensors for detection of phenolic contaminants: strategies, trends, and challenges","authors":"Mei Du , Zecong Yu , Rongxin Lin , Mengfan Niu , Yue Gao , Lei Wang , Xianghao Zha , Maoyong Song , Guibin Jiang","doi":"10.1016/j.trac.2026.118671","DOIUrl":"10.1016/j.trac.2026.118671","url":null,"abstract":"<div><div>Phenolic compounds (PCs) are significant environmental contaminants that pose a serious threat to human health and ecological systems. Developing rapid and accurate monitoring of residual PCs concentrations is essential for effective environmental monitoring. Nanozymes, which combine enzyme-mimicking catalytic activities with the unique physicochemical properties of nanomaterials, have gained significant attention in biosensing. However, a systematic review focused specifically on nanozyme-based biosensors for PCs detection remains lacking. This review systematically summarizes the types and catalytic mechanisms of nanozymes relevant to PCs detection and comprehensively discusses the design principles of biosensors for PCs contaminants. Furthermore, the role of nanozymes in signal transduction and amplification is thoroughly explored across both single and dual detection modalities. Afterwards, the latest nanozyme-integrated sensing platforms are also discussed. Finally, current challenges and future perspectives for intelligent nanozyme-based biosensors in environmental contaminants monitoring are outlined, aiming to promote broader application in related fields.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"196 ","pages":"Article 118671"},"PeriodicalIF":12.0,"publicationDate":"2026-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146034668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-13DOI: 10.1016/j.trac.2026.118672
Mingshi Shao , Shushi Hou , Xiao Fu , Xiang Li , Yu Chen , Pei-Yang Su , Yongchao Huang
Rapid recombination of photogenerated electron-hole pairs limits the efficiency of photoelectrochemical (PEC) water splitting, making understanding charge separation and kinetics diagnostics essential. This review highlights latest advances in multi-spatiotemporal analytical methods for probing charge carrier dynamics, from femtosecond-scale generation to long-term device operation. Time-resolved spectroscopies, including transient absorption spectroscopy (TAS) and time-resolved photoluminescence (TRPL); spatially resolved microscopies, such as Kelvin probe force microscopy (KPFM) and scanning photoelectrochemical microscopy (SPECM); and electrochemical and operando methods, including intensity-modulated photocurrent spectroscopy (IMPS) and operando X-ray absorption spectroscopy (XAS), are compared in terms of principles, resolution, and mechanistic relevance. Emphasis is placed on integrating multimodal and operando characterizations to link atomic-scale charge behavior with macroscopic performance. Complementary computational approaches, including density functional theory (DFT) and machine learning (ML), are also discussed. Finally, future perspectives emphasize multimodal strategies and standardized systems to accelerate rational design of efficient PEC systems for sustainable hydrogen production.
{"title":"Analytical investigation of charge carrier separation in photoelectrochemical water splitting: Multi-Scale methods and emerging trends","authors":"Mingshi Shao , Shushi Hou , Xiao Fu , Xiang Li , Yu Chen , Pei-Yang Su , Yongchao Huang","doi":"10.1016/j.trac.2026.118672","DOIUrl":"10.1016/j.trac.2026.118672","url":null,"abstract":"<div><div>Rapid recombination of photogenerated electron-hole pairs limits the efficiency of photoelectrochemical (PEC) water splitting, making understanding charge separation and kinetics diagnostics essential. This review highlights latest advances in multi-spatiotemporal analytical methods for probing charge carrier dynamics, from femtosecond-scale generation to long-term device operation. Time-resolved spectroscopies, including transient absorption spectroscopy (TAS) and time-resolved photoluminescence (TRPL); spatially resolved microscopies, such as Kelvin probe force microscopy (KPFM) and scanning photoelectrochemical microscopy (SPECM); and electrochemical and operando methods, including intensity-modulated photocurrent spectroscopy (IMPS) and operando X-ray absorption spectroscopy (XAS), are compared in terms of principles, resolution, and mechanistic relevance. Emphasis is placed on integrating multimodal and operando characterizations to link atomic-scale charge behavior with macroscopic performance. Complementary computational approaches, including density functional theory (DFT) and machine learning (ML), are also discussed. Finally, future perspectives emphasize multimodal strategies and standardized systems to accelerate rational design of efficient PEC systems for sustainable hydrogen production.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"196 ","pages":"Article 118672"},"PeriodicalIF":12.0,"publicationDate":"2026-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146034663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-09DOI: 10.1016/j.trac.2026.118667
Paulo Pereira , Emoke Dalma Kovacs , Melinda Haydee Kovacs , Miguel Inácio , Eric C. Brevik , Damia Barcelo
Microplastics and persistent organic pollutants (POPs) harm the environment. Although this topic is widely studied, few works have linked it to soil ecosystem services (ES). Here, we aim to discuss the impacts of microplastics and POPS on soil-regulating, provisioning, and cultural ES. These pollutants increase toxicity, alter biogeochemical processes, and microbial activity, affecting plants' health. This cascade of negative impacts hurts all the soil-regulating ES, including erosion, flood and climate regulation, pollination, air quality regulation, nutrient cycling, carbon sequestration, soil formation, and water purification. Soil degradation and reduced soil productivity also reduce the supply of food, water, biomass, timber, and medicinal resources. Soil pollution and its effects on vegetation degradation negatively affect Cultural ES, such as heritage and landscape aesthetics. It is important to highlight that microplastics and POPs adversely affect all soil-related ES, and it is urgent to develop cost-effective measures to remove them from the environment on a large scale.
{"title":"Microplastics and persistent organic pollutants' impacts on soil ecosystem services supply","authors":"Paulo Pereira , Emoke Dalma Kovacs , Melinda Haydee Kovacs , Miguel Inácio , Eric C. Brevik , Damia Barcelo","doi":"10.1016/j.trac.2026.118667","DOIUrl":"10.1016/j.trac.2026.118667","url":null,"abstract":"<div><div>Microplastics and persistent organic pollutants (POPs) harm the environment. Although this topic is widely studied, few works have linked it to soil ecosystem services (ES). Here, we aim to discuss the impacts of microplastics and POPS on soil-regulating, provisioning, and cultural ES. These pollutants increase toxicity, alter biogeochemical processes, and microbial activity, affecting plants' health. This cascade of negative impacts hurts all the soil-regulating ES, including erosion, flood and climate regulation, pollination, air quality regulation, nutrient cycling, carbon sequestration, soil formation, and water purification. Soil degradation and reduced soil productivity also reduce the supply of food, water, biomass, timber, and medicinal resources. Soil pollution and its effects on vegetation degradation negatively affect Cultural ES, such as heritage and landscape aesthetics. It is important to highlight that microplastics and POPs adversely affect all soil-related ES, and it is urgent to develop cost-effective measures to remove them from the environment on a large scale.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"196 ","pages":"Article 118667"},"PeriodicalIF":12.0,"publicationDate":"2026-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145973576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-08DOI: 10.1016/j.trac.2026.118656
Minzhi Liao , Lu Chen , Yuxuan Yang , Xinyao Yi , Mengyi Xiong , Lin Yuan
Accurate and sensitive molecular detection underpins modern clinical diagnostics and precision medicine. The advent of CRISPR/Cas systems has revolutionized nucleic acid detection, offering programmable specificity and versatile adaptability for clinical biomarker analysis. However, the intrinsically low abundance of disease-associated nucleic acids in clinical samples remains a major barrier, as the native sensitivity of CRISPR-based assays is often insufficient for direct quantification. To bridge this gap and enable clinical translation, extensive efforts have been devoted to enhancing CRISPR detection sensitivity through multifaceted engineering and optimization. Owing to the modular architecture of CRISPR systems, sensitivity can be improved at multiple levels including Cas protein engineering, crRNA design, signal transduction, and reaction condition optimization, many of which can act synergistically. This review provides a systematic overview of recent advances in sensitivity enhancement strategies for CRISPR-based diagnostics and discusses their implications for the development of robust, amplification-free, point-of-care testing platforms. We anticipate that this work will serve as a conceptual framework and technical reference for researchers advancing CRISPR technologies toward clinical applications.
{"title":"From mechanism to sensitivity: Engineering CRISPR/Cas systems for clinical-grade diagnostics","authors":"Minzhi Liao , Lu Chen , Yuxuan Yang , Xinyao Yi , Mengyi Xiong , Lin Yuan","doi":"10.1016/j.trac.2026.118656","DOIUrl":"10.1016/j.trac.2026.118656","url":null,"abstract":"<div><div>Accurate and sensitive molecular detection underpins modern clinical diagnostics and precision medicine. The advent of CRISPR/Cas systems has revolutionized nucleic acid detection, offering programmable specificity and versatile adaptability for clinical biomarker analysis. However, the intrinsically low abundance of disease-associated nucleic acids in clinical samples remains a major barrier, as the native sensitivity of CRISPR-based assays is often insufficient for direct quantification. To bridge this gap and enable clinical translation, extensive efforts have been devoted to enhancing CRISPR detection sensitivity through multifaceted engineering and optimization. Owing to the modular architecture of CRISPR systems, sensitivity can be improved at multiple levels including Cas protein engineering, crRNA design, signal transduction, and reaction condition optimization, many of which can act synergistically. This review provides a systematic overview of recent advances in sensitivity enhancement strategies for CRISPR-based diagnostics and discusses their implications for the development of robust, amplification-free, point-of-care testing platforms. We anticipate that this work will serve as a conceptual framework and technical reference for researchers advancing CRISPR technologies toward clinical applications.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"196 ","pages":"Article 118656"},"PeriodicalIF":12.0,"publicationDate":"2026-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146034659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-08DOI: 10.1016/j.trac.2026.118648
Onur Karaman , Ali İhsan Kömür , Ceren Karaman
Triboelectric nanogenerators (TENGs) are redefining the future of wearable and implantable electronics by enabling energy-autonomous, multifunctional sensing systems. By harvesting ambient mechanical energy and converting it into useful electrical signals, TENGs provide a sustainable solution for powering wearable and implantable devices while simultaneously functioning as active sensors. This review presents a comprehensive perspective on recent advances in TENG technology, with a particular focus on their application in biosensing, bioelectronic, and self-powered health monitoring platforms. We examine fundamental mechanisms of charge generation and transfer, sustainable and biocompatible materials, and surface/interface engineering strategies that enhance triboelectric performance. Special attention is given to hybrid and composite architectures that couple TENGs with piezoelectric, thermoelectric, and photovoltaic systems for continuous power generation and multimodal energy harvesting. Furthermore, the transformative role of artificial intelligence (AI) in augmenting TENG performance, enabling real-time signal processing, intelligent power management, and adaptive biomedical monitoring, is highlighted. Clinical translation and commercialization pathways are also discussed, with an emphasis on biocompatibility, regulatory challenges, and device scalability. Positioned within the One Health framework, this review underscores how battery-free, sustainable, and multifunctional TENG-based biosensing systems can support precision health, environmental and public-health surveillance, and broader sustainable monitoring needs. By integrating energy harvesting, biosensing, and intelligent data processing, TENGs offer a path toward next-generation self-powered bioelectronic systems tailored for precision health, personalized medicine, and broader One Health applications.
{"title":"Triboelectric nanogenerators for self-powered biosensing: Towards intelligent platforms in the one-health framework","authors":"Onur Karaman , Ali İhsan Kömür , Ceren Karaman","doi":"10.1016/j.trac.2026.118648","DOIUrl":"10.1016/j.trac.2026.118648","url":null,"abstract":"<div><div>Triboelectric nanogenerators (TENGs) are redefining the future of wearable and implantable electronics by enabling energy-autonomous, multifunctional sensing systems. By harvesting ambient mechanical energy and converting it into useful electrical signals, TENGs provide a sustainable solution for powering wearable and implantable devices while simultaneously functioning as active sensors. This review presents a comprehensive perspective on recent advances in TENG technology, with a particular focus on their application in biosensing, bioelectronic, and self-powered health monitoring platforms. We examine fundamental mechanisms of charge generation and transfer, sustainable and biocompatible materials, and surface/interface engineering strategies that enhance triboelectric performance. Special attention is given to hybrid and composite architectures that couple TENGs with piezoelectric, thermoelectric, and photovoltaic systems for continuous power generation and multimodal energy harvesting. Furthermore, the transformative role of artificial intelligence (AI) in augmenting TENG performance, enabling real-time signal processing, intelligent power management, and adaptive biomedical monitoring, is highlighted. Clinical translation and commercialization pathways are also discussed, with an emphasis on biocompatibility, regulatory challenges, and device scalability. Positioned within the One Health framework, this review underscores how battery-free, sustainable, and multifunctional TENG-based biosensing systems can support precision health, environmental and public-health surveillance, and broader sustainable monitoring needs. By integrating energy harvesting, biosensing, and intelligent data processing, TENGs offer a path toward next-generation self-powered bioelectronic systems tailored for precision health, personalized medicine, and broader One Health applications.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"196 ","pages":"Article 118648"},"PeriodicalIF":12.0,"publicationDate":"2026-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145973577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-08DOI: 10.1016/j.trac.2026.118664
Xu Fan , Ziwen Zhang , Zhuoting Liu , YingYing Cao , Taojing Zhang , Baofang Feng , Fei Liu , Feng Qu , Shenghang Zhang , Weidong Zhao , Jian Sun , Tie Wang
Surface-enhanced Raman spectroscopy (SERS) is a powerful technique with high sensitivity, fast response, and non-destructive detection, but it faces interference from other substances in practical applications. Aptamers, as excellent affinity agents, have the capability to bind with various targets to form stable structures. The integration of aptamers into SERS sensors can significantly enhance the acquisition and selection of target objects, thereby enabling the detection of various functional molecules. In this review, we describe the mechanisms of SERS enhancement and the construction of SERS substrates. We summarize the design strategies for aptamer-based SERS technology and provide an overview of its applications in environmental pollutant detection, food safety and quality monitoring, and biomedical diagnostics. Finally, we discuss the current advantages and limitations of aptamer-based SERS sensors, as well as the vast potential for future development. This review will provide a guiding route for the development and application of novel aptamer-based sensors.
{"title":"Aptamer-engineered SERS sensors: Molecular recognition meets plasmonic enhancement for precision detection","authors":"Xu Fan , Ziwen Zhang , Zhuoting Liu , YingYing Cao , Taojing Zhang , Baofang Feng , Fei Liu , Feng Qu , Shenghang Zhang , Weidong Zhao , Jian Sun , Tie Wang","doi":"10.1016/j.trac.2026.118664","DOIUrl":"10.1016/j.trac.2026.118664","url":null,"abstract":"<div><div>Surface-enhanced Raman spectroscopy (SERS) is a powerful technique with high sensitivity, fast response, and non-destructive detection, but it faces interference from other substances in practical applications. Aptamers, as excellent affinity agents, have the capability to bind with various targets to form stable structures. The integration of aptamers into SERS sensors can significantly enhance the acquisition and selection of target objects, thereby enabling the detection of various functional molecules. In this review, we describe the mechanisms of SERS enhancement and the construction of SERS substrates. We summarize the design strategies for aptamer-based SERS technology and provide an overview of its applications in environmental pollutant detection, food safety and quality monitoring, and biomedical diagnostics. Finally, we discuss the current advantages and limitations of aptamer-based SERS sensors, as well as the vast potential for future development. This review will provide a guiding route for the development and application of novel aptamer-based sensors.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"196 ","pages":"Article 118664"},"PeriodicalIF":12.0,"publicationDate":"2026-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146034660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-08DOI: 10.1016/j.trac.2026.118666
Qian Liu , Xinyi Tao , Bingqing Nie , Qunlin Zhang , Zikuan Gu , Haifeng Lu
Molecularly imprinted polymers (MIPs) are synthetic affinity materials featuring precisely tailor-made binding cavities complementary to template molecules in shape, size, and functionality. Serving as robust substitutes for biological ligands, MIPs possess distinct advantages including high affinity and specificity, straightforward synthesis, excellent stability and cost-efficiency. Currently, the development of MIPs is undergoing a revolutionary transformation from their traditional applications such as separation, catalysis and biosensing to emerging biomedical frontiers such as bioimaging and nanomedicine. As such, engineering MIP-based nanoparticles (nanoMIPs) have emerged as an appealing platform for precision medicine due to their excellent dispersion, rapid binding kinetics and good biocompatibility. In this review, we summarize the latest strategies for the rational design and advanced fabrication of nanoMIPs. Additionally, the recent progress of nanoMIPs in targeted imaging and therapeutics is presented. Lastly, the potentials and future perspectives of nanoMIPs is discussed. These insights aim to inspire more advanced studies in nanoMIP-based nanomedicines.
{"title":"Recent advances in molecularly imprinted polymer-based nanomedicines for targeted imaging and therapeutics","authors":"Qian Liu , Xinyi Tao , Bingqing Nie , Qunlin Zhang , Zikuan Gu , Haifeng Lu","doi":"10.1016/j.trac.2026.118666","DOIUrl":"10.1016/j.trac.2026.118666","url":null,"abstract":"<div><div>Molecularly imprinted polymers (MIPs) are synthetic affinity materials featuring precisely tailor-made binding cavities complementary to template molecules in shape, size, and functionality. Serving as robust substitutes for biological ligands, MIPs possess distinct advantages including high affinity and specificity, straightforward synthesis, excellent stability and cost-efficiency. Currently, the development of MIPs is undergoing a revolutionary transformation from their traditional applications such as separation, catalysis and biosensing to emerging biomedical frontiers such as bioimaging and nanomedicine. As such, engineering MIP-based nanoparticles (nanoMIPs) have emerged as an appealing platform for precision medicine due to their excellent dispersion, rapid binding kinetics and good biocompatibility. In this review, we summarize the latest strategies for the rational design and advanced fabrication of nanoMIPs. Additionally, the recent progress of nanoMIPs in targeted imaging and therapeutics is presented. Lastly, the potentials and future perspectives of nanoMIPs is discussed. These insights aim to inspire more advanced studies in nanoMIP-based nanomedicines.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"196 ","pages":"Article 118666"},"PeriodicalIF":12.0,"publicationDate":"2026-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145922636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bioimaging, along with embryology is being reshaped by next-generation imaging and analytical technologies. Light-sheet fluorescence microscopy (LSFM), as a leading next-generation technique, provides high-resolution, three-dimensional imaging of embryos over time, surpassing traditional methods such as confocal microscopy, widefield fluorescence microscopy, and Raman microscopy. LSFM has significantly diminished phototoxicity and enabled rapid imaging of entire embryo volumes, enhancing our ability to investigate early-to mid-embryonic development from fertilization through organogenesis in both human and animal models. In parallel with these imaging advancements, artificial intelligence (AI)-driven examination of embryo metabolism is transforming the evaluation of embryo quality and viability, as metabolic activity reflects the embryo's developmental competence and health status. This review analyzes the convergence of LSFM and AI-driven metabolic profiling as a transformative change in reproductive One Health. By integrating next-generation bioimaging into the One Health paradigm, we emphasize its potential to connect fundamental discoveries with cross-species applications in reproductive health.
{"title":"Next-generation bioimaging in reproductive One Health: Light sheet fluorescence microscopy and AI-driven embryo metabolism analysis","authors":"Elaheh Amirinezhadfard , Amirreza Niazi Tabar , Fabrizzio Horta , Wen-Chao Yang","doi":"10.1016/j.trac.2025.118644","DOIUrl":"10.1016/j.trac.2025.118644","url":null,"abstract":"<div><div>Bioimaging, along with embryology is being reshaped by next-generation imaging and analytical technologies. Light-sheet fluorescence microscopy (LSFM), as a leading next-generation technique, provides high-resolution, three-dimensional imaging of embryos over time, surpassing traditional methods such as confocal microscopy, widefield fluorescence microscopy, and Raman microscopy. LSFM has significantly diminished phototoxicity and enabled rapid imaging of entire embryo volumes, enhancing our ability to investigate early-to mid-embryonic development from fertilization through organogenesis in both human and animal models. In parallel with these imaging advancements, artificial intelligence (AI)-driven examination of embryo metabolism is transforming the evaluation of embryo quality and viability, as metabolic activity reflects the embryo's developmental competence and health status. This review analyzes the convergence of LSFM and AI-driven metabolic profiling as a transformative change in reproductive One Health. By integrating next-generation bioimaging into the One Health paradigm, we emphasize its potential to connect fundamental discoveries with cross-species applications in reproductive health.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"196 ","pages":"Article 118644"},"PeriodicalIF":12.0,"publicationDate":"2026-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145922594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-07DOI: 10.1016/j.trac.2026.118661
Bo Peng , Yujie Li , Jichao Qin , Zhuoqun Qiao , Yue Li , Jie Yang , Peiyuan Zhou , Xiaojun Zhao , Ying Kuang , Kao Wu , Fatang Jiang
Timely and accurate monitoring of meat and aquatic product freshness is crucial for public health and food industry. Volatile amine (VA) colorimetric sensors show great potential, but require structural innovation to improve accuracy in complex environments. In this review, we focused on novel structures (porous film, multi-layer film, hydrogel, and aerogel structure) in VA colorimetric sensors. The formation processes and the mechanism for the enhancement in VA sensing performance by these structures were summarized. The macromolecular interactions were the crucial factors that influence sensor structure. By enhancing VA capturing efficiency, optimizing mass transport pathways, and extending diffusion paths of dyes, the sensing performances were improved. Then, recent advances in VA colorimetric sensors for meat and aquatic products’ freshness monitoring were summarized. We also suggested future prospects in this field, including establishing structure-performance relationship, engineering selectivity of the sensor, and using artificial intelligence for sensor design and freshness monitoring in practice.
{"title":"Structure-engineered colorimetric sensors for volatile amine detection: Advances in freshness monitoring of meat and aquatic products","authors":"Bo Peng , Yujie Li , Jichao Qin , Zhuoqun Qiao , Yue Li , Jie Yang , Peiyuan Zhou , Xiaojun Zhao , Ying Kuang , Kao Wu , Fatang Jiang","doi":"10.1016/j.trac.2026.118661","DOIUrl":"10.1016/j.trac.2026.118661","url":null,"abstract":"<div><div>Timely and accurate monitoring of meat and aquatic product freshness is crucial for public health and food industry. Volatile amine (VA) colorimetric sensors show great potential, but require structural innovation to improve accuracy in complex environments. In this review, we focused on novel structures (porous film, multi-layer film, hydrogel, and aerogel structure) in VA colorimetric sensors. The formation processes and the mechanism for the enhancement in VA sensing performance by these structures were summarized. The macromolecular interactions were the crucial factors that influence sensor structure. By enhancing VA capturing efficiency, optimizing mass transport pathways, and extending diffusion paths of dyes, the sensing performances were improved. Then, recent advances in VA colorimetric sensors for meat and aquatic products’ freshness monitoring were summarized. We also suggested future prospects in this field, including establishing structure-performance relationship, engineering selectivity of the sensor, and using artificial intelligence for sensor design and freshness monitoring in practice.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"196 ","pages":"Article 118661"},"PeriodicalIF":12.0,"publicationDate":"2026-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145922593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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