Pub Date : 2024-07-10DOI: 10.1088/2515-7647/ad46a6
Ziming Chen, Robert L Z Hoye, Hin-Lap Yip, Nadesh Fiuza-Maneiro, Iago López-Fernández, Clara Otero-Martínez, Lakshminarayana Polavarapu, Navendu Mondal, Alessandro Mirabelli, Miguel Anaya, Samuel D Stranks, Hui Liu, Guangyi Shi, Zhengguo Xiao, Nakyung Kim, Yunna Kim, Byungha Shin, Jinquan Shi, Mengxia Liu, Qianpeng Zhang, Zhiyong Fan, James C Loy, Lianfeng Zhao, Barry P Rand, Habibul Arfin, Sajid Saikia, Angshuman Nag, Chen Zou, Lih Y Lin, Hengyang Xiang, Haibo Zeng, Denghui Liu, Shi-Jian Su, Chenhui Wang, Haizheng Zhong, Tong-Tong Xuan, Rong-Jun Xie, Chunxiong Bao, Feng Gao, Xiang Gao, Chuanjiang Qin, Young-Hoon Kim and Matthew C Beard
In recent years, the field of metal-halide perovskite emitters has rapidly emerged as a new community in solid-state lighting. Their exceptional optoelectronic properties have contributed to the rapid rise in external quantum efficiencies (EQEs) in perovskite light-emitting diodes (PeLEDs) from <1% (in 2014) to over 30% (in 2023) across a wide range of wavelengths. However, several challenges still hinder their commercialization, including the relatively low EQEs of blue/white devices, limited EQEs in large-area devices, poor device stability, as well as the toxicity of the easily accessible lead components and the solvents used in the synthesis and processing of PeLEDs. This roadmap addresses the current and future challenges in PeLEDs across fundamental and applied research areas, by sharing the community’s perspectives. This work will provide the field with practical guidelines to advance PeLED development and facilitate more rapid commercialization.
{"title":"Roadmap on perovskite light-emitting diodes","authors":"Ziming Chen, Robert L Z Hoye, Hin-Lap Yip, Nadesh Fiuza-Maneiro, Iago López-Fernández, Clara Otero-Martínez, Lakshminarayana Polavarapu, Navendu Mondal, Alessandro Mirabelli, Miguel Anaya, Samuel D Stranks, Hui Liu, Guangyi Shi, Zhengguo Xiao, Nakyung Kim, Yunna Kim, Byungha Shin, Jinquan Shi, Mengxia Liu, Qianpeng Zhang, Zhiyong Fan, James C Loy, Lianfeng Zhao, Barry P Rand, Habibul Arfin, Sajid Saikia, Angshuman Nag, Chen Zou, Lih Y Lin, Hengyang Xiang, Haibo Zeng, Denghui Liu, Shi-Jian Su, Chenhui Wang, Haizheng Zhong, Tong-Tong Xuan, Rong-Jun Xie, Chunxiong Bao, Feng Gao, Xiang Gao, Chuanjiang Qin, Young-Hoon Kim and Matthew C Beard","doi":"10.1088/2515-7647/ad46a6","DOIUrl":"https://doi.org/10.1088/2515-7647/ad46a6","url":null,"abstract":"In recent years, the field of metal-halide perovskite emitters has rapidly emerged as a new community in solid-state lighting. Their exceptional optoelectronic properties have contributed to the rapid rise in external quantum efficiencies (EQEs) in perovskite light-emitting diodes (PeLEDs) from <1% (in 2014) to over 30% (in 2023) across a wide range of wavelengths. However, several challenges still hinder their commercialization, including the relatively low EQEs of blue/white devices, limited EQEs in large-area devices, poor device stability, as well as the toxicity of the easily accessible lead components and the solvents used in the synthesis and processing of PeLEDs. This roadmap addresses the current and future challenges in PeLEDs across fundamental and applied research areas, by sharing the community’s perspectives. This work will provide the field with practical guidelines to advance PeLED development and facilitate more rapid commercialization.","PeriodicalId":44008,"journal":{"name":"Journal of Physics-Photonics","volume":"23 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141586130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-09DOI: 10.1088/2515-7647/ad5bd0
Veerpal Kaur, Demelza Wright, Samuel Mathew, Matthew Peters, Maria Zacharopoulou, Shang Hua Yang, Laura S Itzhaki, Ivet Bahar, Reuven Gordon
We demonstrate the trapping and analysis of individual proteins using a portable optical fiber tweezer setup with a double-nanohole in a gold film coating the fiber’s end and aligned with the fiber core. The instrument was used to trap and analyze cytochrome c, carbonic anhydrase, bovine serum albumin, and PR65 (wild-type and various point mutants). This approach was compared with a free-space optical tweezer setup that requires alignment of the laser focus to the aperture, whereas the fiber-based approach is both portable and alignment-free, which holds promise for applications in antibody discovery, small molecule drug discovery, protein interaction analysis and other applications using the standard well-plate format.
{"title":"Portable fiber-based double nanohole optical tweezer for trapping small proteins","authors":"Veerpal Kaur, Demelza Wright, Samuel Mathew, Matthew Peters, Maria Zacharopoulou, Shang Hua Yang, Laura S Itzhaki, Ivet Bahar, Reuven Gordon","doi":"10.1088/2515-7647/ad5bd0","DOIUrl":"https://doi.org/10.1088/2515-7647/ad5bd0","url":null,"abstract":"We demonstrate the trapping and analysis of individual proteins using a portable optical fiber tweezer setup with a double-nanohole in a gold film coating the fiber’s end and aligned with the fiber core. The instrument was used to trap and analyze cytochrome c, carbonic anhydrase, bovine serum albumin, and PR65 (wild-type and various point mutants). This approach was compared with a free-space optical tweezer setup that requires alignment of the laser focus to the aperture, whereas the fiber-based approach is both portable and alignment-free, which holds promise for applications in antibody discovery, small molecule drug discovery, protein interaction analysis and other applications using the standard well-plate format.","PeriodicalId":44008,"journal":{"name":"Journal of Physics-Photonics","volume":"29 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141571562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-02DOI: 10.1088/2515-7647/ad5bd2
Kamil Gradkowski, Padraic E Morrissey and Peter O’Brien
We propose a method for aligning and attaching micro-lens arrays to photonic integrated circuits (PICs). Unlike the conventional approach of assessing power coupled to a fiber directly, our method utilizes a beam profiler. This profiler allows us to optimize the lens position by analyzing the transmitted beam shape from the PIC edge coupler through the lens. In conjunction, we employ grating couplers to introduce external light, acting as a ‘beacon’ for optimization. The use of grating couplers enables efficient coupling of external light into the PIC, providing a reference point for alignment. Importantly, our method accommodates both regular waveguide-side-up and upside-down (through-Silicon) orientations of the PIC. This versatility allows us to reproduce coupling results across a 6-channel array, demonstrating robust performance. This innovative approach not only ensures precise alignment and attachment but also opens up new possibilities for photonic packaging. The flexibility to work in different orientations is likely to lead to advancements in the design and assembly of photonic devices.
{"title":"Packaging of micro-lens arrays to photonic integrated circuits using beam shape evaluation","authors":"Kamil Gradkowski, Padraic E Morrissey and Peter O’Brien","doi":"10.1088/2515-7647/ad5bd2","DOIUrl":"https://doi.org/10.1088/2515-7647/ad5bd2","url":null,"abstract":"We propose a method for aligning and attaching micro-lens arrays to photonic integrated circuits (PICs). Unlike the conventional approach of assessing power coupled to a fiber directly, our method utilizes a beam profiler. This profiler allows us to optimize the lens position by analyzing the transmitted beam shape from the PIC edge coupler through the lens. In conjunction, we employ grating couplers to introduce external light, acting as a ‘beacon’ for optimization. The use of grating couplers enables efficient coupling of external light into the PIC, providing a reference point for alignment. Importantly, our method accommodates both regular waveguide-side-up and upside-down (through-Silicon) orientations of the PIC. This versatility allows us to reproduce coupling results across a 6-channel array, demonstrating robust performance. This innovative approach not only ensures precise alignment and attachment but also opens up new possibilities for photonic packaging. The flexibility to work in different orientations is likely to lead to advancements in the design and assembly of photonic devices.","PeriodicalId":44008,"journal":{"name":"Journal of Physics-Photonics","volume":"138 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141516849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-01DOI: 10.1088/2515-7647/ad5ae3
Marcus Gutmann, Jana Bachir Salvador, Paul Müller, Kyoohyun Kim, Martin Schicht, Serhii Aif, Friedrich Paulsen, Lorenz Meinel, Jochen Guck and Stephanie Möllmert
Mechanical tissue properties increasingly serve as pivotal phenotypic characteristics that are subject to change during development or pathological progression. The quantification of such material properties often relies on physical contact between a load-applying probe and an exposed sample surface. For most tissues, meeting these requirements entails an invasive preparation, which poses the risk of yielding mechanical properties that do not portray the physiological state of a tissue within a functioning organism. Brillouin microscopy has emerged as a non-invasive, optical technique that enables the assessment of mechanical cell and tissue properties with high spatio-temporal resolution. In optically transparent specimens, it does not require animal sacrifice, tissue dissection or sectioning. However, the extent to which results obtained from Brillouin microscopy allow to infer conclusions about potential results obtained with a contact-based technique, and vice versa, is unclear. Sources for discrepancies include the varying characteristic temporal and spatial scales, the directionality of measurement, environmental factors, and mechanical moduli probed. In this work, we addressed those aspects by quantifying the mechanical properties of acutely dissected murine retinae using Brillouin microscopy and atomic force microscopy (AFM)-based indentation measurements. Our results show a distinct mechanical profile of the retinal layers with respect to the Brillouin frequency shift, the Brillouin linewidth and the apparent Young’s modulus. Contrary to previous reports, our findings do not support a simple correlative relationship between Brillouin frequency shift and apparent Young’s modulus. Additionally, the divergent sensitivities of Brillouin microscopy and AFM-indentation measurements to structural features, as visualized by transmission electron microscopy, to cross-linking or changes post mortem underscore the dangers of assuming interchangeability between the two methods. In conclusion, our study advocates for viewing Brillouin microscopy and AFM-based indentation measurements as complementary tools, discouraging direct comparisons a priori and suggesting their combined use for a more comprehensive understanding of tissue mechanical properties.
{"title":"Beyond comparison: Brillouin microscopy and AFM-based indentation reveal divergent insights into the mechanical profile of the murine retina","authors":"Marcus Gutmann, Jana Bachir Salvador, Paul Müller, Kyoohyun Kim, Martin Schicht, Serhii Aif, Friedrich Paulsen, Lorenz Meinel, Jochen Guck and Stephanie Möllmert","doi":"10.1088/2515-7647/ad5ae3","DOIUrl":"https://doi.org/10.1088/2515-7647/ad5ae3","url":null,"abstract":"Mechanical tissue properties increasingly serve as pivotal phenotypic characteristics that are subject to change during development or pathological progression. The quantification of such material properties often relies on physical contact between a load-applying probe and an exposed sample surface. For most tissues, meeting these requirements entails an invasive preparation, which poses the risk of yielding mechanical properties that do not portray the physiological state of a tissue within a functioning organism. Brillouin microscopy has emerged as a non-invasive, optical technique that enables the assessment of mechanical cell and tissue properties with high spatio-temporal resolution. In optically transparent specimens, it does not require animal sacrifice, tissue dissection or sectioning. However, the extent to which results obtained from Brillouin microscopy allow to infer conclusions about potential results obtained with a contact-based technique, and vice versa, is unclear. Sources for discrepancies include the varying characteristic temporal and spatial scales, the directionality of measurement, environmental factors, and mechanical moduli probed. In this work, we addressed those aspects by quantifying the mechanical properties of acutely dissected murine retinae using Brillouin microscopy and atomic force microscopy (AFM)-based indentation measurements. Our results show a distinct mechanical profile of the retinal layers with respect to the Brillouin frequency shift, the Brillouin linewidth and the apparent Young’s modulus. Contrary to previous reports, our findings do not support a simple correlative relationship between Brillouin frequency shift and apparent Young’s modulus. Additionally, the divergent sensitivities of Brillouin microscopy and AFM-indentation measurements to structural features, as visualized by transmission electron microscopy, to cross-linking or changes post mortem underscore the dangers of assuming interchangeability between the two methods. In conclusion, our study advocates for viewing Brillouin microscopy and AFM-based indentation measurements as complementary tools, discouraging direct comparisons a priori and suggesting their combined use for a more comprehensive understanding of tissue mechanical properties.","PeriodicalId":44008,"journal":{"name":"Journal of Physics-Photonics","volume":"36 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141531176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-27DOI: 10.1088/2515-7647/ad5775
Bahareh Mastiani, Daniël W S Cox and Ivo M Vellekoop
Wavefront shaping (WFS) is a technique for directing light through turbid media. The theoretical aspects of WFS are well understood, and under near-ideal experimental conditions, accurate predictions for the expected signal enhancement can be given. In practice, however, there are many experimental factors that negatively affect the outcome of the experiment. Here, we present a comprehensive overview of these experimental factors, including the effect of sample scattering properties, noise, and response of the spatial light modulator. We present simple means to identify experimental imperfections and to minimize their negative effect on the outcome of the experiment. This paper is accompanied by Python code for automatically quantifying experimental problems using the OpenWFS framework for running and simulating WFS experiments.
{"title":"Practical considerations for high-fidelity wavefront shaping experiments","authors":"Bahareh Mastiani, Daniël W S Cox and Ivo M Vellekoop","doi":"10.1088/2515-7647/ad5775","DOIUrl":"https://doi.org/10.1088/2515-7647/ad5775","url":null,"abstract":"Wavefront shaping (WFS) is a technique for directing light through turbid media. The theoretical aspects of WFS are well understood, and under near-ideal experimental conditions, accurate predictions for the expected signal enhancement can be given. In practice, however, there are many experimental factors that negatively affect the outcome of the experiment. Here, we present a comprehensive overview of these experimental factors, including the effect of sample scattering properties, noise, and response of the spatial light modulator. We present simple means to identify experimental imperfections and to minimize their negative effect on the outcome of the experiment. This paper is accompanied by Python code for automatically quantifying experimental problems using the OpenWFS framework for running and simulating WFS experiments.","PeriodicalId":44008,"journal":{"name":"Journal of Physics-Photonics","volume":"128 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141516850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-23DOI: 10.1088/2515-7647/ad5772
Diego Quintero Balbas, Laura Maestro-Guijarro, Paula María Carmona-Quiroga, Mohamed Oujja, Marta Castillejo, Francesca Bettini, Simone Porcinai and Jana Striova
Stratigraphic analyzes of polychrome surfaces, such as paintings, often need samples to offer consistent results regarding the sequence and composition of the layers. Non-invasive methodologies based on linear and nonlinear optical techniques limit material removal from the objects. Recently, optical coherence tomography (OCT) has become the preferred choice of heritage scientists because it is a safe and fast alternative for studying transparent or semi-transparent layers. Yet, nonlinear optical microscopy (NLOM)) technique in its modality of multiphoton excitation fluorescence (MPEF) has emerged as a promising tool for the same purpose. Here, we explored linear (OCT and confocal Raman microspectroscopy (CRM)) and nonlinear (NLOM-MPEF) optical methods’ capability to investigate gelatine-based layers in mock-up samples and a painting dated 1939 by an artist from the Surrealistic entourage. The optical behavior of mock-up samples that imitate the painting stratigraphy and of six painting fragments detached from the support was also investigated with fiber optics reflectance spectroscopy and laser induced fluorescence (LIF). Thickness values from the mock-ups obtained with OCT, CRM, and MPEF have provided evidence of the complementarity, from a millimetric to a micrometric scale, and the limitations (e.g. strong fluorescence emission in CRM) of the methods. Moreover, the presence of gelatine was ascertained by LIF spectroscopy applied to the painting fragments and NLOM-MPEF confirmed its suitability as a non-invasive technique for investigating gelatine-based stratigraphic systems.
{"title":"Non-invasive stratigraphic analyzes of gelatine-based modern painting materials with linear and nonlinear optical methods","authors":"Diego Quintero Balbas, Laura Maestro-Guijarro, Paula María Carmona-Quiroga, Mohamed Oujja, Marta Castillejo, Francesca Bettini, Simone Porcinai and Jana Striova","doi":"10.1088/2515-7647/ad5772","DOIUrl":"https://doi.org/10.1088/2515-7647/ad5772","url":null,"abstract":"Stratigraphic analyzes of polychrome surfaces, such as paintings, often need samples to offer consistent results regarding the sequence and composition of the layers. Non-invasive methodologies based on linear and nonlinear optical techniques limit material removal from the objects. Recently, optical coherence tomography (OCT) has become the preferred choice of heritage scientists because it is a safe and fast alternative for studying transparent or semi-transparent layers. Yet, nonlinear optical microscopy (NLOM)) technique in its modality of multiphoton excitation fluorescence (MPEF) has emerged as a promising tool for the same purpose. Here, we explored linear (OCT and confocal Raman microspectroscopy (CRM)) and nonlinear (NLOM-MPEF) optical methods’ capability to investigate gelatine-based layers in mock-up samples and a painting dated 1939 by an artist from the Surrealistic entourage. The optical behavior of mock-up samples that imitate the painting stratigraphy and of six painting fragments detached from the support was also investigated with fiber optics reflectance spectroscopy and laser induced fluorescence (LIF). Thickness values from the mock-ups obtained with OCT, CRM, and MPEF have provided evidence of the complementarity, from a millimetric to a micrometric scale, and the limitations (e.g. strong fluorescence emission in CRM) of the methods. Moreover, the presence of gelatine was ascertained by LIF spectroscopy applied to the painting fragments and NLOM-MPEF confirmed its suitability as a non-invasive technique for investigating gelatine-based stratigraphic systems.","PeriodicalId":44008,"journal":{"name":"Journal of Physics-Photonics","volume":"24 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141516851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-19DOI: 10.1088/2515-7647/ad5774
Ronen Shekel, Kfir Sulimany, Shachar Resisi, Zohar Finkelstein, Ohad Lib, Sébastien M Popoff and Yaron Bromberg
Multimode optical fibers support the dense, low-loss transmission of many spatial modes, making them attractive for technologies such as communications and imaging. However, information propagating through multimode fibers is scrambled, due to modal dispersion and mode mixing. This is usually rectified using wavefront shaping techniques with devices such as spatial light modulators. Recently, we demonstrated an all-fiber system for controlling light propagation inside multimode fibers using mechanical perturbations, called the fiber piano. In this tutorial we explain the design considerations and experimental methods needed to build a fiber piano, and review applications where fiber pianos have been used.
{"title":"Tutorial: How to build and control an all-fiber wavefront modulator using mechanical perturbations","authors":"Ronen Shekel, Kfir Sulimany, Shachar Resisi, Zohar Finkelstein, Ohad Lib, Sébastien M Popoff and Yaron Bromberg","doi":"10.1088/2515-7647/ad5774","DOIUrl":"https://doi.org/10.1088/2515-7647/ad5774","url":null,"abstract":"Multimode optical fibers support the dense, low-loss transmission of many spatial modes, making them attractive for technologies such as communications and imaging. However, information propagating through multimode fibers is scrambled, due to modal dispersion and mode mixing. This is usually rectified using wavefront shaping techniques with devices such as spatial light modulators. Recently, we demonstrated an all-fiber system for controlling light propagation inside multimode fibers using mechanical perturbations, called the fiber piano. In this tutorial we explain the design considerations and experimental methods needed to build a fiber piano, and review applications where fiber pianos have been used.","PeriodicalId":44008,"journal":{"name":"Journal of Physics-Photonics","volume":"59 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141507338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-07DOI: 10.1088/2515-7647/ad50b2
Alessandra Anna Passeri, Chiara Argentati, Francesco Morena, Francesco Bonacci, Igor Neri, Daniele Fioretto, Massimo Vassalli, Sabata Martino, Maurizio Mattarelli, Silvia Caponi
Brillouin spectroscopy has recently attracted attention as a powerful tool for the characterization of the mechanical properties of heterogeneous materials, particularly in the biological and biomedical domains. This study investigates the procedure to use Brillouin data to provide relevant morphological parameters of micro-structured samples. When acquiring Brillouin spectra at the interface between two regions of the sample, the spectrum shows signatures of both regions. This feature can be used to precisely identify the position of the interfaces by analyzing the evolution of the fitting parameters of the Brillouin spectra acquired by performing a linear scan across the interface. This concept has been demonstrated by measuring the thickness of adherent HEK293T cells. The results are validated using fluorescence microscopy, showing an excellent agreement. The present analysis showcases the wealth of information present in the Brillouin spectrum and the potentiality of Brillouin spectroscopy not only for mechanical characterization but also for label-free, high-resolution imaging of sample morphology. The study introduces the possibility of correlating mechanical properties and shape of biological samples using a single technique.
{"title":"Brillouin spectroscopy for accurate assessment of morphological and mechanical characteristics in micro-structured samples","authors":"Alessandra Anna Passeri, Chiara Argentati, Francesco Morena, Francesco Bonacci, Igor Neri, Daniele Fioretto, Massimo Vassalli, Sabata Martino, Maurizio Mattarelli, Silvia Caponi","doi":"10.1088/2515-7647/ad50b2","DOIUrl":"https://doi.org/10.1088/2515-7647/ad50b2","url":null,"abstract":"Brillouin spectroscopy has recently attracted attention as a powerful tool for the characterization of the mechanical properties of heterogeneous materials, particularly in the biological and biomedical domains. This study investigates the procedure to use Brillouin data to provide relevant morphological parameters of micro-structured samples. When acquiring Brillouin spectra at the interface between two regions of the sample, the spectrum shows signatures of both regions. This feature can be used to precisely identify the position of the interfaces by analyzing the evolution of the fitting parameters of the Brillouin spectra acquired by performing a linear scan across the interface. This concept has been demonstrated by measuring the thickness of adherent HEK293T cells. The results are validated using fluorescence microscopy, showing an excellent agreement. The present analysis showcases the wealth of information present in the Brillouin spectrum and the potentiality of Brillouin spectroscopy not only for mechanical characterization but also for label-free, high-resolution imaging of sample morphology. The study introduces the possibility of correlating mechanical properties and shape of biological samples using a single technique.","PeriodicalId":44008,"journal":{"name":"Journal of Physics-Photonics","volume":"12 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141546792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-06DOI: 10.1088/2515-7647/ad50b1
Patrick Cameron, Baptiste Courme, Daniele Faccio, Hugo Defienne
Quantum imaging enhances imaging systems performance, potentially surpassing fundamental limits such as noise and resolution. However, these schemes have limitations and are still a long way from replacing classical techniques. Therefore, there is a strong focus on improving the practicality of quantum imaging methods, with the goal of finding real-world applications. With this in mind, in this tutorial we describe how the concepts of classical light shaping can be applied to imaging schemes based on entangled photon pairs. We detail two basic experimental configurations in which a spatial light modulator is used to shape the spatial correlations of a photon pair state and highlight the key differences between this and classical shaping. We then showcase two recent examples that expand on these concepts to perform aberration and scattering correction with photon pairs. We include specific details on the key steps of these experiments, with the goal that this can be used as a guide for building photon-pair-based imaging and shaping experiments.
{"title":"Shaping the spatial correlations of entangled photon pairs","authors":"Patrick Cameron, Baptiste Courme, Daniele Faccio, Hugo Defienne","doi":"10.1088/2515-7647/ad50b1","DOIUrl":"https://doi.org/10.1088/2515-7647/ad50b1","url":null,"abstract":"Quantum imaging enhances imaging systems performance, potentially surpassing fundamental limits such as noise and resolution. However, these schemes have limitations and are still a long way from replacing classical techniques. Therefore, there is a strong focus on improving the practicality of quantum imaging methods, with the goal of finding real-world applications. With this in mind, in this tutorial we describe how the concepts of classical light shaping can be applied to imaging schemes based on entangled photon pairs. We detail two basic experimental configurations in which a spatial light modulator is used to shape the spatial correlations of a photon pair state and highlight the key differences between this and classical shaping. We then showcase two recent examples that expand on these concepts to perform aberration and scattering correction with photon pairs. We include specific details on the key steps of these experiments, with the goal that this can be used as a guide for building photon-pair-based imaging and shaping experiments.","PeriodicalId":44008,"journal":{"name":"Journal of Physics-Photonics","volume":"15 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141546793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-02DOI: 10.1088/2515-7647/ad4cc6
Caterina Czibula, Manfred H Ulz, Alexander Wagner, Kareem Elsayad, Ulrich Hirn and Kristie J Koski
Brillouin light scattering spectroscopy is applied to study the micromechanics of cellulosic viscose fibers, one of the commercially most important, man-made biobased fibers. Using an equal angle scattering geometry, we provide a thorough description of the procedure to determine the complete transversely isotropic elastic stiffness tensor. From the stiffness tensor the engineering-relevant material parameters such as Young’s moduli, shear moduli, and Poisson’s ratios in radial and axial fiber direction are evaluated. The investigated fiber type shows that, at ideal conditions, the material exhibits optical waveguide properties resulting in spontaneous Brillouin backscattering which can be used to obtain additional information from the Brillouin spectra, enabling the measurement of two different scattering processes and directions with only one scattering geometry.
{"title":"The elastic stiffness tensor of cellulosic viscose fibers measured with Brillouin spectroscopy","authors":"Caterina Czibula, Manfred H Ulz, Alexander Wagner, Kareem Elsayad, Ulrich Hirn and Kristie J Koski","doi":"10.1088/2515-7647/ad4cc6","DOIUrl":"https://doi.org/10.1088/2515-7647/ad4cc6","url":null,"abstract":"Brillouin light scattering spectroscopy is applied to study the micromechanics of cellulosic viscose fibers, one of the commercially most important, man-made biobased fibers. Using an equal angle scattering geometry, we provide a thorough description of the procedure to determine the complete transversely isotropic elastic stiffness tensor. From the stiffness tensor the engineering-relevant material parameters such as Young’s moduli, shear moduli, and Poisson’s ratios in radial and axial fiber direction are evaluated. The investigated fiber type shows that, at ideal conditions, the material exhibits optical waveguide properties resulting in spontaneous Brillouin backscattering which can be used to obtain additional information from the Brillouin spectra, enabling the measurement of two different scattering processes and directions with only one scattering geometry.","PeriodicalId":44008,"journal":{"name":"Journal of Physics-Photonics","volume":"63 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141252856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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