Biomedical Spectroscopy and Imaging最新文献

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The use of FTIR and Raman spectroscopy in combination with chemometrics for analysis of biomolecules in biomedical fluids: A review FTIR和拉曼光谱与化学计量学相结合用于分析生物医学流体中的生物分子：综述

Biomedical Spectroscopy and Imaging

Pub Date : 2020-01-27 DOI: 10.3233/bsi-200189

A. Rohman, A. Windarsih, E. Lukitaningsih, M. Rafi, K. Betania, N. Fadzillah

Fourier transform infrared (FTIR) and Raman spectroscopy are complementary techniques, typically called vibrational spectroscopy. Both techniques allow simple, rapid, non-destructive, specific, providing fingerprint spectra, and real-time analytical method for analysis of molecules in different states. Besides, these methods are simple without any excessive sample pre-treatment, therefore, they are sometimes called as “green analytical methods”. Biofluids have several biomolecules such as lipid, protein, nucleic acids, and carbohydrates. These biomolecules can be used as biomarkers to detect some types of diseases, since biomolecules are in direct contact with the human organs. FTIR and Raman spectra of biofluids are complex in nature, therefore sophisticated statistical techniques, known as chemometrics, must be used to solve the analytical problems related to quantitative analysis purposes. The objective of this review is to show the capability of FTIR and Raman spectroscopic techniques in combination with chemometrics techniques to analyze the biomolecules in biofluids through an extensive literature review. During performing this review, several databases in Science citation index, Scopus PubMed, and Google Scholar related to the topics are identified and downloaded. With the present review, it is known that FTIR and Raman techniques are rapid method for screening certain diseases by identifying the level changes of some biomolecules. In the future, this method will be widely used for clinicians as new diagnostic tools for many diseases.

傅立叶变换红外光谱（FTIR）和拉曼光谱是互补的技术，通常称为振动光谱。这两种技术都允许简单、快速、无损、特异、提供指纹光谱和实时分析方法来分析不同状态的分子。此外，这些方法简单，没有任何过度的样品预处理，因此，它们有时被称为“绿色分析方法”。生物流体有几种生物分子，如脂质、蛋白质、核酸和碳水化合物。这些生物分子可以用作检测某些类型疾病的生物标志物，因为生物分子与人体器官直接接触。生物流体的FTIR和拉曼光谱本质上是复杂的，因此必须使用复杂的统计技术，即化学计量学，来解决与定量分析目的相关的分析问题。这篇综述的目的是通过广泛的文献综述，展示FTIR和拉曼光谱技术与化学计学技术相结合分析生物流体中生物分子的能力。在进行本综述期间，确定并下载了《科学》引文索引、Scopus PubMed和谷歌学者中与主题相关的几个数据库。通过本综述，已知FTIR和拉曼技术是通过识别某些生物分子的水平变化来筛选某些疾病的快速方法。在未来，这种方法将作为许多疾病的新诊断工具被临床医生广泛使用。

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引用次数: 35

A review of applications of Raman spectroscopy in immunology 拉曼光谱在免疫学中的应用综述

Biomedical Spectroscopy and Imaging

Pub Date : 2020-01-01 DOI: 10.3233/bsi-200198

N. Chaudhary, Claire Wynne, A. Meade

Vibrational spectroscopic techniques have recently gained increasing clinical importance as non-invasive, rapid and inexpensive methods to obtain information on the content of biological samples. For some time Raman spectroscopy has been involved in preclinical applications, mainly in the cancer space, with evolving applications towards new horizons in the dermatology and companion diagnostics arena. It is attractive as an analytical technique due to its exquisite sensitivity, labelfree operation and low water detectivity such that in-vivo applications are possible. In cytometry, Raman spectroscopy has been applied to the analysis of single cells providing a label-free alternative to cell classification approaches in the laboratory. In this review we collate in-vitro, ex-vivo and in-vivo examples of research using Raman spectroscopy for the detection, quantification and analysis of immune signaling at the cellular level. While cancer biology has recently focussed on the role of immunological signals in the development of the disease, it is timely to examine these applications as research in this space evolves.

近年来，振动光谱技术作为一种无创、快速、廉价的获取生物样品含量信息的方法，在临床中具有越来越重要的意义。一段时间以来，拉曼光谱已经参与临床前应用，主要是在癌症领域，随着皮肤病学和伴随诊断领域的应用向新的领域发展。它是一种有吸引力的分析技术，由于其精致的灵敏度，无标签操作和低水检测，使体内应用成为可能。在细胞术中，拉曼光谱已被应用于单个细胞的分析，为实验室中的细胞分类方法提供了一种无标记的替代方法。在这篇综述中，我们整理了利用拉曼光谱在细胞水平上检测、定量和分析免疫信号的体外、离体和体内研究实例。虽然癌症生物学最近关注免疫信号在疾病发展中的作用，但随着这一领域研究的发展，检查这些应用是及时的。

引用次数: 4

The Potential of Utilizing Mid-Energy X-Rays for In-Line Phase Sensitive Breast Cancer Imaging. 利用中能量x射线在线相敏乳腺癌成像的潜力。

Biomedical Spectroscopy and Imaging

Pub Date : 2020-01-01 Epub Date: 2020-12-28 DOI: 10.3233/BSI-200204

F H Omoumi, M U Ghani, M D Wong, Y Li, B Zheng, A Yan, P A Jenkins, X Wu, H Liu

Objective: The objective of this study is to demonstrate the potential of utilizing mid-energy x-rays for in-line phase-sensitive breast cancer imaging by phantom studies.

Methods: The midenergy (50-80kV) in-line phase sensitive imaging prototype was used to acquire images of the contrast-detail mammography (CDMAM) phantom, an ACR accreditation phantom, and an acrylic edge phantom. The low-dose mid-energy phase-sensitive images were acquired at 60 kV with a radiation dose of 0.9 mGy, while the high-energy phase-sensitive images were acquired at 90 kV with a radiation dose of 1.2 mGy. The Phase-Attenuation Duality (PAD) principle for soft tissue was used for the phase retrieval. A blind observer study was conducted and paired-sample T-test were performed to compare the mean differences in the two imaging systems.

Results: The correct detection ratio for the CDMAM phantom for phase-contrast images acquired by the low-dose mid-energy system was 56.91%, whereas images acquired by the high-energy system correctly revealed only 40.97% of discs. The correct detection ratios were 57.88% and 43.41% for phase-retrieved images acquired by the low-dose mid-energy and high-energy imaging systems, respectively. The reading scores for all three groups of objects in the ACR phantom were higher for the mid energy imaging system as compared to the high-energy system for both phase-contrast and phase- retrieved images. The calculated edge enhancement index (EEI) from the acrylic edge phantom image for the mid-energy system was higher than that calculated for the high-energy imaging system. The quantitative analyses showed a higher Contrast to Noise Ratio (CNR) as well as a higher Figure of Merit (FOM) in images acquired by the low-dose mid-energy imaging system.

Conclusion: The PAD based retrieval method can be applied in mid-energy system without remarkably affecting the image quality, and in fact, it improves the lesion detectability with a patient dose saving of 25%.

目的:本研究的目的是通过假体研究证明利用中能量x射线在线相敏乳腺癌成像的潜力。方法:采用中能量(50-80kV)在线相敏成像样机获取对比细节乳房x线造影(CDMAM)幻像、ACR认证幻像和丙烯酸边缘幻像图像。在60 kV、0.9 mGy的辐射剂量下获得了低剂量中能量相敏图像，在90 kV、1.2 mGy的辐射剂量下获得了高能相敏图像。利用软组织相位衰减对偶性(PAD)原理进行相位恢复。采用盲观察研究，采用配对样本t检验比较两种成像系统的平均差异。结果:低剂量中能量系统对CDMAM幻像的相对比成像正确率为56.91%，而高能系统对CDMAM幻像的正确率仅为40.97%。低剂量、中能量和高能成像系统获取的相位反演图像的正确率分别为57.88%和43.41%。与高能成像系统相比，中能量成像系统对ACR幻影中所有三组物体的阅读分数都更高，无论是相位对比还是相位检索图像。中能量系统中亚克力边缘虚像计算得到的边缘增强指数(EEI)高于高能成像系统计算得到的边缘增强指数。定量分析表明，低剂量中能量成像系统获得的图像具有较高的噪比(CNR)和较高的优值(FOM)。结论:基于PAD的检索方法可应用于中能量系统，对图像质量不产生明显影响，实际上提高了病灶的可检出性，可节省患者剂量25%。

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引用次数: 1

Label-free digital pathology by infrared imaging 红外成像无标签数字病理

Biomedical Spectroscopy and Imaging

Pub Date : 2020-01-01 DOI: 10.3233/bsi-200196

F. Großerueschkamp, K. Gerwert

引用次数: 4

A gold nanoparticle conjugate with photosystem I and photosystem II for development of a biohybrid water-splitting photocatalyst 金纳米粒子与光系统I和光系统II偶联用于开发生物杂化水分解光催化剂

Biomedical Spectroscopy and Imaging

Pub Date : 2020-01-01 DOI: 10.3233/bsi-200200

Kousuke Kawahara, Natsuko Inoue-Kahino, Keisuke Namie, Yuki Kato, T. Tomo, Y. Shibata, Y. Kashino, T. Noguchi

引用次数: 2

Novel application of confocal Raman microscopy to determine the microstructure of fermented dairy products including the spatial distribution of proteins, lipids and carbohydrates 新应用的共聚焦拉曼显微镜来确定发酵乳制品的微观结构，包括蛋白质，脂质和碳水化合物的空间分布

Biomedical Spectroscopy and Imaging

Pub Date : 2020-01-01 DOI: 10.3233/bsi-200201

Anita P. Pax, J. Sheehan

引用次数: 4

Optimization of optical properties of Ba0.2Sr0.8TiO3 thin films for a glucose sensor implementation 用于葡萄糖传感器实现的Ba0.2Sr0.8TiO3薄膜光学性能优化

Biomedical Spectroscopy and Imaging

Pub Date : 2020-01-01 DOI: 10.3233/bsi-200197

E. Palupi, H. Alatas, Irzaman, Y. Suryana, A. Aridarma, R. Umam, B. B. Andriana, H. Sato

引用次数: 0

Determining T2 relaxation time and stroke onset relationship in ischaemic stroke within apparent diffusion coefficient-defined lesions. A user-independent method for quantifying the impact of stroke in the human brain. 在表观扩散系数定义的病变范围内确定缺血性卒中的T2弛豫时间和卒中发作关系。一种独立于用户的方法，用于量化中风对人脑的影响。

Biomedical Spectroscopy and Imaging

Pub Date : 2019-07-09 DOI: 10.3233/BSI-190185

Michael J. Knight, Robin A. Damion, Bryony L. McGarry, R. Bosnell, K. Jokivarsi, O. Gröhn, P. Jezzard, G. Harston, D. Carone, J. Kennedy, S. El-Tawil, Jennifer Elliot, K. Muir, P. Clatworthy, R. Kauppinen

Background and ObjectiveIn hyperacute ischaemic stroke, T2 of cerebral water increases with time. Quantifying this change may be informative of the extent of tissue damage and onset time. Our objective was to develop a user-unbiased method to measure the effect of cerebral ischaemia on T2 to study stroke onset time-dependency in human acute stroke lesions.MethodsSix rats were subjected to permanent middle cerebral occlusion to induce focal ischaemia, and a consecutive cohort of acute stroke patients (n = 38) were recruited within 9 hours from symptom onset. T1-weighted structural, T2 relaxometry, and diffusion MRI for apparent diffusion coefficient (ADC) were acquired. Ischaemic lesions were defined as regions of lowered ADC. The median T2 difference (ΔT2) between lesion and contralateral non-ischaemic control region was determined by the newly-developed spherical reference method, and data compared to that obtained by the mirror reference method. Linear regressions and receiver operating characteristics (ROC) were compared between the two methods.ResultsΔT2 increases linearly in rat brain ischaemia by 1.9 ± 0.8 ms/h during the first 6 hours, as determined by the spherical reference method. In patients, ΔT2 linearly increases by 1.6 ± 1.4 and 1.9 ± 0.9 ms/h in the lesion, as determined by the mirror reference and spherical reference method, respectively. ROC analyses produced areas under the curve of 0.83 and 0.71 for the spherical and mirror reference methods, respectively.ConclusionsData from the spherical reference method showed that the median T2 increase in the ischaemic lesion is correlated with stroke onset time in a rat as well as in a human patient cohort, opening the possibility of using the approach as a timing tool in clinics.

背景与目的在超急性缺血性脑卒中中，脑水T2随时间增加。量化这种变化可能是组织损伤程度和发病时间的信息。我们的目的是开发一种用户无偏的方法来测量脑缺血对T2的影响，以研究人类急性脑卒中病变的脑卒中发病时间依赖性。方法对6只大鼠进行永久性中脑闭塞以诱导局灶性缺血，并在症状出现后9小时内招募连续的急性脑卒中患者（n=38）。获得T1加权结构、T2弛豫测量和表观扩散系数（ADC）的扩散MRI。缺血性病变被定义为ADC降低的区域。病变和对侧非缺血性对照区之间的中位T2差（ΔT2）通过新开发的球面参考法确定，并将数据与镜像参考法获得的数据进行比较。比较了两种方法之间的线性回归和受试者操作特性（ROC）。结果大鼠脑缺血的ΔT2在前6小时内线性增加1.9±0.8 ms/h，通过球形参考法测定。在患者中，ΔT2在病变中线性增加1.6±1.4和1.9±0.9 ms/h，分别通过镜像参考和球面参考方法确定。ROC分析在球面和镜面参考法的曲线下分别产生0.83和0.71的面积。结论球形参考方法的数据显示，在大鼠和人类患者队列中，缺血性病变的中位T2增加与中风发作时间相关，这为在临床中使用该方法作为计时工具提供了可能性。

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引用次数: 3

Impact of a single distance phase retrieval algorithm on spatial resolution in X-ray inline phase sensitive imaging. 单距离相位检索算法对x射线内联相敏成像空间分辨率的影响。

Biomedical Spectroscopy and Imaging

Pub Date : 2019-07-09 DOI: 10.3233/BSI-190186

Muhammad U. Ghani, Bradley Gregory, Farid H Omoumi, B. Zheng, A. Yan, Xizeng Wu, Hong Liu

A single-projection based phase retrieval method based on the phase attenuation duality principle (PAD) was used to compare the spatial resolution of the acquired phase sensitive and PAD processed phase retrieved images. An inline phase sensitive prototype was used to acquire the phase sensitive images. The prototype incorporates a micro-focus x-ray source and a flat panel detector with a 50 μm pixel pitch. A phantom composed of a 2 cm thick 50-50 adipose-glandular mimicking slab sandwiched with a 0.82 cm thick slanted PMMA sharp edge was used. Phase sensitive image of the phantom was acquired at 120 kV, 3.35 mAs with a 16 μm tube focal spot size under a geometric magnification (M) of 2.5. The PAD based method was applied to the acquired phase sensitive image for the retrieval of phase values. With necessary data processing, modulation transfer function (MTF) curves were determined for the estimation and comparison of the spatial resolution. The PAD processed phase retrieved values of the phantom were in good agreement with the theoretically calculated values. Phase sensitive images showed higher spatial resolution at all spatial frequencies compared to the phase retrieved images. It was noted that the high-frequency signal components in the retrieved image were suppressed that resulted in lower MTF values. When compared to the phase sensitive image, the cutoff resolution (10% MTF) for phase retrieved image dropped 32% from 15.6 lp/mm (32μm) to 10.6 lp/mm (47μm). The resolution offered by this phase sensitive prototype is radiographically enough to detect breast cancer.

使用基于相位衰减对偶原理（PAD）的基于单投影的相位检索方法来比较采集的相位敏感图像和PAD处理的相位检索图像的空间分辨率。使用在线相位敏感原型来获取相位敏感图像。原型包括一个微焦点x射线源和一个像素间距为50μm的平板探测器。使用由2cm厚的50-50脂肪腺体模拟板组成的体模，该板夹有0.82cm厚的倾斜PMMA锐边。在几何放大倍数（m）为2.5的情况下，在120 kV、3.35 mAs、16μm管焦斑大小下采集体模的相位敏感图像。将基于PAD的方法应用于采集的相位敏感图像，以检索相位值。通过必要的数据处理，确定了调制传递函数（MTF）曲线，用于空间分辨率的估计和比较。PAD处理的体模相位检索值与理论计算值非常一致。与相位检索图像相比，相位敏感图像在所有空间频率下都显示出更高的空间分辨率。注意到检索到的图像中的高频信号分量被抑制，这导致较低的MTF值。与相位敏感图像相比，相位检索图像的截止分辨率（10%MTF）从15.6lp/mm（32μm）下降了32%，降至10.6LP/mm（47μm）。这个相敏原型提供的分辨率在射线照相上足以检测癌症。

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引用次数: 6

The effect of polymer and gold functionalization on the magnetic properties of magnetite nanoparticles 聚合物和金功能化对磁铁矿纳米粒子磁性能的影响

Biomedical Spectroscopy and Imaging

Pub Date : 2019-01-24 DOI: 10.3233/BSI-180182

Staci Adams, Sara Bonabi, A’Lester C. Allen, G. Roseman, A. P. Ramirez, G. Millhauser, Jin Z. Zhang

引用次数: 5

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