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The Dark Room Problem: Scapegoating and Audience Complicity in Twelfth Night 暗室问题:《第十二夜》中的欺骗与观众共谋
IF 0.3 4区 心理学 Q3 PSYCHOLOGY, PSYCHOANALYSIS Pub Date : 2023-07-04 DOI: 10.1080/07351690.2023.2221624
Christopher W. T. Miller
ABSTRACT In Twelfth Night (1601–02), the pressure to enforce a comedic ending competes with problematic and overtly abusive behaviors towards certain characters, particularly the steward Malvolio, who is identified as a target for scapegoating by a persecuting group. As Malvolio is subject to mounting pressures to take in the projections of the group, the mistreatment levied against him gains in cruelty, culminating with his imprisonment in a dark room. While the “pranksters” seek to drive him mad, the figures in power ensure their own narcissistically satisfying ending, at the expense of those stripped of control and dignity. The role of the audience becomes a complex matter by the end, as applauding a problematic play meant to be funny invites a degree of collusion with those who would enforce scapegoating dynamics.
摘要在《第十二夜》(1601-02)中,强制执行喜剧结局的压力与对某些角色的问题和公开虐待行为相竞争,尤其是管家马尔沃利奥,他被迫害团体认定为替罪羊的目标。由于马尔沃里奥承受着越来越大的压力,需要接受该组织的预测,对他的虐待愈演愈烈,最终导致他被监禁在黑暗的房间里。当“恶作剧者”试图把他逼疯时,当权者以牺牲那些被剥夺控制权和尊严的人为代价,确保了他们自己自恋的令人满意的结局。到最后,观众的角色变成了一个复杂的问题,因为为一部本应搞笑的有问题的戏剧鼓掌,会在一定程度上与那些实施替罪羊动态的人勾结。
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引用次数: 0
Love’s Labor’s Lost: The Lived-Experience, A Pan-European Play 爱的劳动的失败:生活的经验,一个泛欧洲的戏剧
IF 0.3 4区 心理学 Q3 PSYCHOLOGY, PSYCHOANALYSIS Pub Date : 2023-07-04 DOI: 10.1080/07351690.2023.2221628
Elisabeth Waugaman
ABSTRACT This article addresses Abel Lefranc’s analysis of Love’s Labor’s Lost. Lefranc was a member of the Académie française, a highly respected Renaissance specialist, who published Behind the Mask of William Shakespeare in 1918. Nothing in Shakespere of Stratford’s hundreds of business records or will (the only records we have) reveal any knowledge of French. He never travelled abroad. He was a teenager in Stratford when the events in the play take place. He possibly began to learn French when he roomed with a French Huguenot family in London at the age of thirty-eight in 1602; however, Hamlet, based on an untranslated French source, was presented in 1593. Love’s Labor’s Lost (1594–98) reveals a detailed knowledge of historical events at the French court of Nérac from 1578 to 1582 and presents important, as well as minor French historical characters, and even a suppressed scandal – a wealth of knowledge not available to the general public. There is no known primary source for the play. Whoever wrote the play knew in great detail what was happening at the court of Nérac between 1578–1582. Lefranc did a phenomenal amount of research to reveal the forgotten history behind the play – the lived experience and its pan-European vision. Restoring the history reveals the play’s rich psychological depth.
本文论述了亚伯·勒弗兰克对爱的劳动迷失的分析。Lefranc是文艺复兴时期备受尊敬的专家法国学院的成员,他于1918年出版了《威廉·莎士比亚的面具背后》。斯特拉特福德的Shakespere的数百份商业记录或遗嘱(我们唯一的记录)中没有任何内容透露法语知识。他从未出国旅行。剧中事件发生时,他还是斯特拉特福德的一名青少年。1602年,三十八岁的他可能在伦敦与一个法国胡格诺派家庭同住时开始学习法语;然而,《哈姆雷特》是根据一个未经翻译的法语来源于1593年推出的。Love’s Labor’s Lost(1594–98)揭示了1578年至1582年法国内拉克宫廷历史事件的详细信息,并呈现了重要和次要的法国历史人物,甚至是一个被压制的丑闻——这是公众无法获得的丰富知识。这出戏没有已知的主要来源。无论是谁写的剧本,都非常详细地了解1578-1582年间内拉克宫廷发生的事情。Lefranc做了大量的研究,揭示了这部剧背后被遗忘的历史——生活经历及其泛欧洲愿景。还原历史,展现出该剧丰富的心理深度。
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引用次数: 0
Prologue: Fictional Characters: Psychoanalytic and Literary Perspectives 前言:虚构人物:精神分析和文学视角
IF 0.3 4区 心理学 Q3 PSYCHOLOGY, PSYCHOANALYSIS Pub Date : 2023-07-04 DOI: 10.1080/07351690.2023.2221623
R. Waugaman
Are fictional characters real people? Of course not. By definition. Even when they’re partially inspired by one or more actual people. But if they’re not real, why do we care about them enough to read fiction? Further, why has there been such a profound disconnect for the past several decades between general readers’ reactions to fictional characters, and those of many literary theorists, who tell us such characters are merely words on the page, and that it is absurd to speculate about their psychology, or their backstory? In college, I took a comparative literature seminar on novels that openly or – more often – subtly alluded in their plots to the life of Jesus. That is, the novels’ protagonists had the life of Jesus as a salient backdrop, adding depth to their characters. Our teacher later published a book based on the topic of our seminar (Ziolkowski, 1972). That course sensitized me to the possibility that a fictional character can have an important backstory – including one of literary allusion – that will enrich our experience with the book. This issue was inspired by a book review. Evan Kindley of Pomona College, in the March 25, 2021 New York Review of Books, wrote an essay titled “The People We Know Best” (Kindley, 2021). Below the title appeared these provocative and generative statements: “Readers love fictional characters almost as if they were real people. Literary scholars are just starting to take them more seriously.” This tapped into longstanding concerns I have had about the direction that literary theory has taken, downplaying the roles of actual writers and readers of fiction. In the process, it has weakened its previously robust connection with a truly psychoanalytic view of the mind. After citing a wide range of opinions about the nature of fictional characters, Kindley concludes, “A strange fact about academic literary criticism is that this . . . view [that of L.C. Knights, who wrote that fictional characters are ‘merely an abstraction . . . in the mind of the reader’] – that literary characters don’t even exist – has been the predominant one for almost a century, despite being the least intuitively satisfying and attractive one to most people. Indeed, literary critics have been strict about policing readers (and one another) when it comes to talking about character” (accessed online September 17, 2022). Psychoanalysts are bound to a have special interest in studying in depth what fiction and fictional characters mean to readers, including readers who are our patients (for example, Novey, 1964). Ours is a developmental theory, so we would expect to find a developmental line of our experiences with stories, beginning with being read to by a parent; reading picture books, then chapter books as a child; then encountering adult fiction as an adolescent and beyond. Children spend much time in the world of their imagination (Selma Fraiberg, 1959, The Magic Years). Fraiberg contends that “language originates in magic” (p. 112),
虚构人物是真人吗?当然不是。根据定义。即使他们的部分灵感来自一个或多个真实的人。但如果它们不是真实的,我们为什么要关心它们来读小说呢?此外,为什么在过去的几十年里,普通读者对虚构人物的反应与许多文学理论家的反应之间存在着如此深刻的脱节,他们告诉我们,这些人物只是页面上的文字,推测他们的心理或背景故事是荒谬的?在大学里,我参加了一个关于小说的比较文学研讨会,这些小说在情节中公开或更频繁地微妙地暗示了耶稣的生活。也就是说,小说的主人公以耶稣的生活为突出背景,为他们的人物增加了深度。我们的老师后来根据我们研讨会的主题出版了一本书(Ziolkowski,1972)。这门课让我意识到,一个虚构人物可能有一个重要的背景故事,包括一个文学典故,这将丰富我们对这本书的体验。本期的灵感来自一篇书评。波莫纳学院的Evan Kindley在2021年3月25日的《纽约书评》上写了一篇题为《我们最了解的人》的文章(Kindley,2021)。标题下面出现了这些挑衅性的、富有创造性的陈述:“读者喜欢虚构人物,就好像他们是真实的人一样。文学学者才刚刚开始更认真地对待他们。”这利用了我长期以来对文学理论方向的担忧,淡化了真实作家和小说读者的角色。在这个过程中,它削弱了以前与真正的精神分析观点的牢固联系。在引用了关于虚构人物本质的广泛观点后,Kindley总结道,“学术文学批评的一个奇怪事实是,这种……观点[L.C.Knights的观点,他写道虚构人物‘只是读者心目中的抽象’]尽管对大多数人来说,文学人物是最不直观、最不吸引人的,但近一个世纪以来,文学人物一直是主流。事实上,文学评论家在谈论人物时一直严格要求读者(以及彼此)的监督”(2022年9月17日在线访问)。精神分析家必然对深入研究小说和虚构人物对读者意味着什么特别感兴趣,包括我们的病人(例如,Novey,1964)。我们的理论是一种发展理论,所以我们希望从父母的阅读开始,找到我们的故事经历的发展路线;小时候读图画书,然后读章节书;然后在青少年时期和以后遇到成人小说。孩子们花了很多时间在他们想象的世界里(Selma Fraiberg,1959,the Magic Years)。Fraiberg认为,“语言源于魔法”(第112页),因为婴儿牙牙学语的“魔法咒语”有时会“带来一个想要的事件”,比如当他第一次说“妈妈”时,母亲脸上露出灿烂的笑容。当成年人读小说取乐时,童年的自我状态会神奇地复活。威廉·施莱格尔(Wilhelm Schlegel)观察到,莎士比亚尤其将我们与我们想象力的童年来源联系在一起,这样我们就可以更好地理解和欣赏他的戏剧(“对童年来说……有吸引力和可理解的故事……它们甚至将成年带回了想象力的黄金时代,”施莱格尔,1815年,第387页)。多年来,我与一位英语教授朋友进行了许多富有启发性的对话,他也接受过许多精神分析方面的非临床培训。我们都热爱创意
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引用次数: 0
Still Alice, Always Elena: New Stories about Dementia 仍然是爱丽丝,永远是埃琳娜:关于痴呆症的新故事
IF 0.3 4区 心理学 Q3 PSYCHOLOGY, PSYCHOANALYSIS Pub Date : 2023-07-04 DOI: 10.1080/07351690.2023.2221627
Jehanne M. Gheith
ABSTRACT As a hospice social worker and tenured professor of Russian literature, I have seen how the stories we tell and re-tell affect how patients, caregivers, and medical professionals live with many different diseases. My essay explores one aspect of this: how the stories we tell about dementia shape our understanding and treatment of cognitive decline. Through an analysis of the novels Still Alice (by Lisa Genova) and The Kukotsky Case (by Liudmila Ulitskaia; English translation by Diane Nemec Ignashev, 2016), I show how telling a story about the possibilities of dementia can help us to reshape our conception of dementia and that, in turn, can change the experience of caregivers, patients, and medical professionals. I argue that fiction can be a powerful agent of transformation, both personal, and, in time, societal. A deep dive into two novels and one condition reveals more than a broader analysis would as these two novels demand close readings. The implications of the essay are larger as I invite medical professionals to think about story and narrative skill in understanding all kinds of disease-and indeed, how “medicalizing” and/or pathologizing conditions such as dementia can work against deeper connections and engagement.
摘要作为一名临终关怀社会工作者和俄罗斯文学终身教授,我看到了我们讲述和重新讲述的故事如何影响患者、护理人员和医疗专业人员如何与许多不同的疾病共存。我的文章探讨了其中的一个方面:我们讲述的关于痴呆症的故事如何影响我们对认知能力下降的理解和治疗。通过对小说《依然爱丽丝》(Lisa Genova著)和《库科茨基案》(Liudmila Ulitskaia著;Diane Nemec Ignashev英译,2016年)的分析,我展示了讲述痴呆症可能性的故事如何帮助我们重塑痴呆症的概念,进而改变护理人员、患者和医疗专业人员的体验。我认为,小说可以成为一种强大的变革推动者,无论是个人的,还是社会的。深入研究两部小说和一个条件,会发现这两部小说需要仔细阅读,而不是更广泛的分析。这篇文章的意义更大,因为我邀请医学专业人士思考故事和叙事技巧,以理解各种疾病,以及如何将痴呆症等疾病“医学化”和/或病理化,以对抗更深层次的联系和参与。
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引用次数: 0
Our Lives in Literature 我们的文学生活
IF 0.3 4区 心理学 Q3 PSYCHOLOGY, PSYCHOANALYSIS Pub Date : 2023-07-04 DOI: 10.1080/07351690.2023.2221626
P. Cohen
ABSTRACT The article traces the evolution of literary theory, beginning in the 1970s when it moved away from the New Criticism that saw the text as removed from the life of its readers toward Reader-Response Theory, where readers are encouraged to see themselves in what they read. I argue that this theory of reading leads to an awareness of how literature helps us fashion a coherent self. I use three examples – the Bible, Shakespeare, and certain nineteenth-century novels – to argue that great literature is great because it gives us the material we need to do this well.
本文追溯了文学理论的演变,从20世纪70年代开始,它从新批评主义转向读者反应理论,在新批评主义中,文本脱离了读者的生活,读者反应理论鼓励读者在他们所读的东西中看到自己。我认为这种阅读理论让我们意识到文学是如何帮助我们塑造连贯的自我的。我举了三个例子——《圣经》、莎士比亚和某些19世纪的小说——来证明,伟大的文学之所以伟大,是因为它提供了我们做好这件事所需的材料。
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引用次数: 0
Origins of Fictional Characters: Creating Life on the Page 小说人物的起源:在页面上创造生活
IF 0.3 4区 心理学 Q3 PSYCHOLOGY, PSYCHOANALYSIS Pub Date : 2023-07-04 DOI: 10.1080/07351690.2023.2221625
Fred L Griffin
ABSTRACT There are many perspectives from which the origins of fictional characters may be explored. In this essay the author draws from the voices of a selected group of creative writers who openly describe the manner in which their life experience and personal psychology play important roles in the creative process giving rise to their fictional characters. For some writers whose experience of loss or trauma informs this process, the ongoing act of writing is felt to be essential.
摘要:可以从许多角度来探讨虚构人物的起源。在这篇文章中,作者从一组被选中的创意作家的声音中汲取灵感,他们公开描述了他们的生活经历和个人心理在创作过程中扮演重要角色的方式,从而塑造了他们的虚构人物。对于一些经历过损失或创伤的作家来说,持续的写作行为被认为是至关重要的。
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引用次数: 0
Epilogue: Fictional Characters: Psychoanalytic and Literary Perspectives 结语:小说人物:精神分析与文学视角
IF 0.3 4区 心理学 Q3 PSYCHOLOGY, PSYCHOANALYSIS Pub Date : 2023-07-04 DOI: 10.1080/07351690.2023.2221630
R. Waugaman
The story goes that some learned medieval monks were debating the question of how many teeth horses had, a seemingly crucial issue, on which Aristotle was silent. Overhearing the debate, an illiterate lay brother suggested that he could go outside where a horse was tied up, and count its teeth. The monks naturally rebuked him for his effrontery. Similarly, English literature scholars have many debates about novels and whether it’s legitimate to connect them with their authors. Fred Griffin is clearly not illiterate, and I’m not aware he’s ever been attacked for his efforts to provide answers to some of these literary debates, but, like the lay brother, he decided to investigate some of these questions empirically, “letting the authors speak for themselves,” rather than to treat theory reductionistically as a “Procrustean bed.” Griffin notes the “therapeutic potentials” of writing fiction, citing Virginia Woolf’s comment that writing To the Lighthouse helped her grieve her mother’s death. Griffin learned from personal experience that transforming painful experiences into fiction allows enough emotional distance to deepen one’s understanding of trauma. After writing of similar themes in the works of William Maxwell, Arnost Lustig, Elizabeth YoungBruehl, and Colin Toibin, Griffin presents his fascinating interview with the novelist and short story writer Sheila Kohler. In some ways, Christopher Miller’s entire essay persuasively glosses Malvolio’s final words in Twelfth Night, which bring the mirthful audience up short: “I’ll be revenged on the whole pack of you.” Miller probes deeply into the play’s complexity, since Shakespeare never fully separates comic from darker, tragic themes in his dramas. Miller skillfully shows how we, the audience, implicitly collude in scapegoating Malvolio, as other characters in the play – whom Miller compares with “pack animals” – projectively identify some of their “despised elements” such as self-love onto him, and limit our empathy for Malvolio. Mistreating Malvolio, through the dynamics of groupthink (“the group’s mindset”), gives the other characters – as well as the audience – a sense of cohesive group identity. Miller reads Shakespeare’s words closely, just as we try to listen closely to our patient’s words. He refers to Malvolio’s temporary “descent into hell,” as he’s locked in a small room and gaslighted. We might remember that ancient heroic figures such as Odysseus, Orpheus, and Jesus also visited the underworld and, like Malvolio, returned from it. Toby may be alluding to purgatory in claiming that Malvolio is in the dark room for his “penance.” Paula Marantz Cohen’s highly compressed article opens with a sad tale of an irrational putdown of her sophomore essay from her theory-obsessed college English professor, accusing Cohen of “confusing fictional characters with real people.” She quickly learned to write what her teachers wanted, rather than allowing her own heart to inspire her. She takes us on a to
据说,一些有学识的中世纪僧侣正在争论马有多少牙齿的问题,这似乎是一个至关重要的问题,亚里士多德对此保持沉默。一个不识字的兄弟听到了这场辩论,建议他可以到拴着马的地方去数它的牙齿。僧侣们很自然地斥责他厚颜无耻。同样,英国文学学者对小说以及将其与作者联系起来是否合法也有很多争论。弗雷德·格里芬显然不是文盲,我也不知道他曾因努力为一些文学辩论提供答案而受到攻击,但和这位外行兄弟一样,他决定实证研究其中一些问题,“让作者自己说话”,而不是将理论还原论视为“普鲁斯特式的床”。格里芬指出了写小说的“治疗潜力”,引用了弗吉尼亚·伍尔夫的评论,即写《致灯塔》帮助她为母亲的去世感到悲伤。格里芬从个人经历中学到,将痛苦的经历转化为小说可以让人有足够的情感距离来加深对创伤的理解。在威廉·马克斯韦尔、阿诺斯特·卢斯蒂格、伊丽莎白·扬布鲁尔和科林·托宾的作品中都有类似的主题之后,格里芬对小说家和短篇小说作家希拉·科勒进行了引人入胜的采访。在某种程度上,克里斯托弗·米勒的整篇文章令人信服地掩盖了马尔沃利奥在《第十二夜》中的最后一句话,这句话让欢乐的观众感到失望:“我会为你们所有人复仇。”米勒深入探讨了这部剧的复杂性,因为莎士比亚在他的戏剧中从未完全区分喜剧和黑暗、悲剧的主题。米勒巧妙地展示了我们观众是如何暗中串通将马尔沃利奥作为替罪羊的,就像剧中的其他角色——米勒将其比作“驮畜”——投射地将他们的一些“被鄙视的元素”,如自爱,归咎于他,并限制了我们对马尔沃利奥的同情。通过群体思维(“群体心态”)的动态,对马尔沃里奥进行错误的创作,给其他角色——以及观众——一种有凝聚力的群体认同感。米勒仔细阅读莎士比亚的话,就像我们试图仔细倾听病人的话一样。他指的是马尔沃里奥暂时“坠入地狱”,因为他被锁在一个小房间里,用煤气灯照明。我们可能还记得,奥德修斯、俄耳甫斯和耶稣等古代英雄人物也曾访问过阴间,并像马尔沃利奥一样从那里回来。托比声称马尔沃利奥在黑暗的房间里“忏悔”,可能是在暗指炼狱。Paula Marantz Cohen的这篇高度压缩的文章以一个悲伤的故事开场,讲述了她痴迷于理论的大学英语教授对她大二的文章的非理性贬低,指责Cohen“将虚构人物与真人混为一谈”。她很快学会了写老师想要的东西,而不是让自己的心来激励她。她在读研究生院时,带我们参观了文学批评不断变化的时尚,后来她自己也成为了一名英语教授。科恩从不允许自己被当今的理论洗脑。她发现阅读反应理论是最合适的理论。她有说服力地得出结论:“与其说我们现在能看到文学人物的真实,不如说我们能看到自己像文学人物一样。”她将家庭代际反犹太主义创伤与对亚伯拉罕和艾萨克的评论联系起来。科恩描绘了一条文学发展路线,从莎士比亚的独白到19世纪英国小说中人物突出的内心生活——用科恩令人难忘的话来说,是“阴险的保姆”。在她引人注目的结尾
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引用次数: 0
The Origins of Modern Literary Theory in the Repudiation of Autobiographical Readings of Shakespeare’s Sonnets 从对莎士比亚十四行诗自传体解读的否定看现代文学理论的起源
IF 0.3 4区 心理学 Q3 PSYCHOLOGY, PSYCHOANALYSIS Pub Date : 2023-07-04 DOI: 10.1080/07351690.2023.2221629
R. Waugaman
ABSTRACT For a century, prevailing literary theories have amputated authors from their fictional works, reducing characters to merely words on the page. Many of us who love reading literature are puzzled by the disconnect between our subjective assumptions about it and those of literary critics, who reject common-sense understanding of the vital role of the author, and of the interaction between our imagination and fictional characters. A major but unacknowledged reason for this counterintuitive trend has been efforts to buttress the traditional but increasingly dubious legend about who wrote the works of William Shakespeare. Since the late 1500s, there have been doubts as to the identity of the real Shakespeare. His name was often hyphenated as Shake-speare in early years, when hyphenated last names in England were rare then. But assumed names in plays were sometimes hyphenated. Sir Sidney Lee was one of the most prominent and influential Shakespeare scholars at the turn of the 20th century. He looked for biographical clues about Shakespeare in his Sonnets. But he quickly did an about-face in reaction to the bisexuality of these Sonnets. Lee’s reversal was pivotal in literary critics’ subsequent dogma that Shakespeare’s works, and fiction in general, need to be separated from the author. This irrational dogma was also applied to fictional characters, in part in reaction to reductionistic uses of psychoanalytic theory to “analyze” literary characters. The close study of this history may help free us from misconceptions about the real Shakespeare, and from misguided literary theory.
摘要一个世纪以来,主流的文学理论已经将作者从他们的虚构作品中剔除,将人物简化为页面上的文字。我们中的许多热爱阅读文学的人都对我们对文学的主观假设与文学评论家的主观假设之间的脱节感到困惑,文学评论家拒绝对作者的重要角色以及我们的想象力与虚构人物之间的互动进行常识性理解。这种违反直觉的趋势的一个主要但未被承认的原因是,人们努力支持关于谁写了威廉·莎士比亚作品的传统但越来越可疑的传说。自15世纪末以来,人们一直怀疑真正的莎士比亚的身份。早年,他的名字经常连字符为Shake speare,当时连字符的姓氏在英国很少见。但剧中的假名有时会连字符。西德尼·李爵士是20世纪之交最杰出、最有影响力的莎士比亚学者之一。他在十四行诗中寻找有关莎士比亚的传记线索。但他很快就对这些十四行诗中的双性恋做出了改变。李的逆转在文学评论家随后的教条中起到了关键作用,即莎士比亚的作品和一般小说都需要与作者分离。这种非理性教条也适用于虚构人物,部分原因是对精神分析理论“分析”文学人物的还原论使用的反应。仔细研究这段历史可能有助于我们摆脱对真正莎士比亚的误解,以及被误导的文学理论。
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引用次数: 0
Single-cell and spatiotemporal transcriptomic analyses reveal the effects of microorganisms on immunity and metabolism in the mouse liver. 单细胞和时空转录组分析揭示了微生物对小鼠肝脏免疫和新陈代谢的影响。
IF 6 4区 心理学 Q3 PSYCHOLOGY, PSYCHOANALYSIS Pub Date : 2023-06-30 eCollection Date: 2023-01-01 DOI: 10.1016/j.csbj.2023.06.020
Ruizhen Zhao, Wei Cheng, Juan Shen, Weiming Liang, Zhao Zhang, Yifei Sheng, Tailiang Chai, Xueting Chen, Yin Zhang, Xiang Huang, Huanjie Yang, Chunqing Song, Li Pang, Cuoji Nan, Yangrui Zhang, Rouxi Chen, Junpu Mei, Hong Wei, Xiaodong Fang

The gut-liver axis is a complex bidirectional communication pathway between the intestine and the liver in which microorganisms and their metabolites flow from the intestine through the portal vein to the liver and influence liver function. In a sterile environment, the phenotype or function of the liver is altered, but few studies have investigated the specific cellular and molecular effects of microorganisms on the liver. To this end, we constructed single-cell and spatial transcriptomic (ST) profiles of germ-free (GF) and specific-pathogen-free (SPF) mouse livers. Single-cell RNA sequencing (scRNA-seq) and single-nucleus RNA sequencing (snRNA-seq) revealed that the ratio of most immune cells was altered in the liver of GF mice; in particular, natural killer T (NKT) cells, IgA plasma cells (IgAs) and Kupffer cells (KCs) were significantly reduced in GF mice. Spatial enhanced resolution omics sequencing (Stereo-seq) confirmed that microorganisms mediated the accumulation of Kupffer cells in the periportal zone. Unexpectedly, IgA plasma cells were more numerous and concentrated in the periportal vein in liver sections from SPF mice but less numerous and scattered in GF mice. ST technology also enables the precise zonation of liver lobules into eight layers and three patterns based on the gene expression level in each layer, allowing us to further investigate the effects of microbes on gene zonation patterns and functions. Furthermore, untargeted metabolism experiments of the liver revealed that the propionic acid levels were significantly lower in GF mice, and this reduction may be related to the control of genes involved in bile acid and fatty acid metabolism. In conclusion, the combination of sc/snRNA-seq, Stereo-seq, and untargeted metabolomics revealed immune system defects as well as altered bile acid and lipid metabolic processes at the single-cell and spatial levels in the livers of GF mice. This study will be of great value for understanding host-microbiota interactions.

肠肝轴是肠道和肝脏之间复杂的双向交流途径,其中微生物及其代谢物从肠道通过门静脉流向肝脏并影响肝脏功能。在无菌环境中,肝脏的表型或功能会发生改变,但很少有研究调查微生物对肝脏的具体细胞和分子影响。为此,我们构建了无菌(GF)和无特定病原体(SPF)小鼠肝脏的单细胞和空间转录组(ST)图谱。单细胞RNA测序(scRNA-seq)和单核RNA测序(snRNA-seq)显示,GF小鼠肝脏中大多数免疫细胞的比例发生了改变;特别是,GF小鼠的自然杀伤T细胞(NKT)、IgA浆细胞(IgAs)和Kupffer细胞(KCs)明显减少。空间增强分辨率全息测序(Stereo-seq)证实,微生物介导了 Kupffer 细胞在门静脉周围区域的聚集。出乎意料的是,在 SPF 小鼠的肝脏切片中,IgA 浆细胞数量更多,且集中在门静脉周围,而在 GF 小鼠中,IgA 浆细胞数量较少且分散。ST 技术还能根据各层的基因表达水平将肝小叶精确分区为八层和三种模式,使我们能够进一步研究微生物对基因分区模式和功能的影响。此外,肝脏非靶向代谢实验显示,GF 小鼠的丙酸水平显著降低,这种降低可能与胆汁酸和脂肪酸代谢相关基因的控制有关。总之,结合 sc/snRNA-seq、Stereo-seq 和非靶向代谢组学发现了 GF 小鼠肝脏中免疫系统的缺陷以及单细胞和空间水平上胆汁酸和脂质代谢过程的改变。这项研究对于了解宿主与微生物群之间的相互作用具有重要价值。
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引用次数: 0
Dead Men Walking 行尸走肉
IF 0.3 4区 心理学 Q3 PSYCHOLOGY, PSYCHOANALYSIS Pub Date : 2023-05-19 DOI: 10.1080/07351690.2023.2193541
J. Paddock
ABSTRACT In this article, I write of an 11-year odyssey with a patient who despite our best efforts, remained mired in emotional deadness, anomie, and depersonalization. The journey led me to question my core assumptions about co-creating an effective therapeutic alliance as well as my competence as an experienced psychotherapist-psychologist, well-trained in empirically validated treatments. What I realized was a failed treatment, that led me to pursue psychoanalytic training. In the process, I grew to understand that our parallel traumatic life histories, and my inadvertent and unconscious reluctance to acknowledge my own countertransference, kept me from joining with him in the intersubjective experience of profound grief – that which, in retrospect, I believe would have made all the difference in his treatment.
摘要在这篇文章中,我写了一个11年的冒险之旅,一个病人尽管我们尽了最大的努力,但仍然深陷于情感的麻木、失范和人格解体之中。这段旅程让我质疑我关于共同创建有效治疗联盟的核心假设,以及我作为一名经验丰富的心理治疗师和心理学家的能力,在经验验证的治疗方面训练有素。我意识到的是一次失败的治疗,这让我开始接受心理分析训练。在这个过程中,我逐渐明白,我们平行的创伤生活史,以及我无意中和无意识地不愿承认自己的反移情,使我无法与他一起经历深刻悲伤的主体间体验——回想起来,我相信这会对他的治疗产生很大影响。
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Psychoanalytic Inquiry
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