Quantifying soil organic carbon (SOC) and total soil nitrogen (TSN) at a cumulative depth of 0–30 cm under different land use and management scenarios remains a priority, particularly in the southeastern United States, where soil erosion can be intense with high rainfall and long history of intensive soil disturbance. Restoration of soil health is expected with conservation management, and sensitive indicators of change may be from SOC, TSN, and the active fraction of organic matter as soil-test biological activity (STBA). Cumulative frequency distributions were developed from data in published studies containing 1470 soil profiles that characterized SOC, TSN, and STBA under cropland, grassland, and woodland in the southeastern United States. Soil health targets were established at ratios of root-zone enrichment-to-baseline SOC and TSN of 2.0 ± 0.1 kg kg−1; 4.7 ± 0.2 kg kg−1 was the target for STBA. This database serves as a reference to begin classifying root-zone soil health conditions in the southeastern United States.