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Yeast-Mediated Biomass Valorization for Biofuel Production: A Literature Review 酵母介导生物燃料生产的生物质增值:文献综述
IF 3.7 3区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2023-08-24 DOI: 10.3390/fermentation9090784
V. Ahuja, A. Arora, Shikhabaghel Chauhan, S. Thakur, C. Jeyaseelan, D. Paul
The European Union has recommended that about 10–50% of the global energy requirement should be supplemented by waste biomass resources by 2050 in order to achieve the objective of having net-zero-emission economies. This has led to intensive research being conducted on developing appropriate biofuel production technologies using advanced or integrated systems to tackle local, national, and global energy challenges using waste feedstock. Researchers have realized the potential of microbes (e.g., yeast strains) for bioenergy production. For this paper, both non-oleaginous and oleaginous yeasts were reviewed, with a specific focus being placed on their diversity in metabolism and tolerance to the various challenges that arise from the use of waste feedstock and influence bioprocessing. Gathering in-depth knowledge and information on yeast metabolism has paved the way for newer and better technologies to employ them for consolidated biorefineries to not only produce biofuels but also to cut down process expenses and decrease the risks of net carbon emissions. The rationale for using yeast strains improved by metabolic engineering and genetic manipulation that can substantially meet the challenges of alternate fuel resources is also described in this paper. This literature review presents the advantages and disadvantages of yeast-based biofuel production and highlights the advancements in technologies and how they contrast to conventional methods. Over the last decade, scientific publications have endorsed the idea of biorefineries for environmentally friendly, cost-effective, and sustainable biofuel production.
欧盟建议,到2050年,全球约10%至50%的能源需求应由废弃生物质资源补充,以实现净零排放经济的目标。这导致人们对利用先进或集成系统开发适当的生物燃料生产技术进行了深入研究,以利用废物原料应对地方、国家和全球的能源挑战。研究人员已经意识到微生物(例如酵母菌株)在生物能源生产方面的潜力。本文对非含油酵母和含油酵母进行了综述,重点介绍了它们在代谢和耐受性方面的多样性,以应对因使用废物原料和影响生物加工而产生的各种挑战。收集有关酵母代谢的深入知识和信息为更新更好的技术铺平了道路,将其用于综合生物精炼厂,不仅可以生产生物燃料,还可以减少工艺费用,降低净碳排放的风险。本文还描述了使用通过代谢工程和遗传操作改进的酵母菌株的基本原理,这些菌株可以充分满足替代燃料资源的挑战。这篇文献综述介绍了酵母生物燃料生产的优点和缺点,并强调了技术的进步以及它们与传统方法的对比。在过去的十年里,科学出版物支持了生物精炼厂的理念,以实现环境友好、成本效益高和可持续的生物燃料生产。
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引用次数: 0
Joint Multi-Optimization of an Extremophilic Microbial Bioanode for Mitigation of Mixed Hazardous Azo Dyes in Textile Synthetic Wastewater 嗜极微生物生物阳极处理纺织合成废水中有害偶氮染料的联合优化研究
IF 3.7 3区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2023-08-24 DOI: 10.3390/fermentation9090782
Sirine Saadaoui, B. Erable, L. Etchevery, A. Cherif, H. Chouchane
Bioelectrochemical systems (BESs), rather than physicochemical processes, are used for wastewater remediation, electricity production, and zero carbon dioxide emission. Textile effluents contain organic and inorganic compounds that can fuel BESs. The main goal of this study was to understand the interplay between the anode material, its surface area, the potential applied to the working electrode (WE), and the concentration of the co-substrate, and how these factors lead to the formation of highly efficient thermohalophilic bioanodes (THB) retrieved from Chott El Djerid (SCD) hypersaline sediment for the treatment of synthetic textile wastewater. To this end, twenty-seven bioanode formation experiments were designed using a Box-Behnken matrix and response surface methodology to understand concomitant interactions. All experiments were conducted in electrochemical reactors of final volume 750 mL inoculated with 80% of enrichment medium containing three azo dyes at a concentration of 300 ppm and 20% of biocatalyst microbial SCD source, at 45 °C. The optimal levels were predicted using NemrodW software as carbon felt (CF) anode material, 6 cm2 anode surface, 7 g/L glucose concentration, and −0.1 V applied potential. These theoretical results were experimentally validated, using maximum current output of 5.23 ± 0.30 A/m2, decolorization rate of 100%, and a chemical oxygen demand (COD) removal rate of 96 ± 1%. Illumina Miseq results revealed that bacterial community harbored the bioanode was dominated at phylum level by Firmicutes (67.1%). At the species level, the biofilm was mainly colonized by Orenia metallireducens species (59.5%). Obtained findings show a promising application of THB in the degradation of recalcitrant molecules as well as for the energy recovery.
生物电化学系统(BES),而不是物理化学过程,用于废水修复、电力生产和零二氧化碳排放。纺织废水含有可为BES提供燃料的有机和无机化合物。本研究的主要目标是了解阳极材料、其表面积、施加到工作电极(WE)的电势和共基质的浓度之间的相互作用,以及这些因素如何导致从Chott El Djerid(SCD)高盐沉积物中回收的高效热卤性生物节点(THB)的形成,用于处理合成纺织废水。为此,使用Box-Behnken矩阵和响应面方法设计了27个生物节点形成实验,以了解伴随的相互作用。所有实验均在最终体积为750mL的电化学反应器中进行,在45°C下接种80%的含有浓度为300ppm的三种偶氮染料和20%的生物催化剂微生物SCD源的富集介质。使用NemrodW软件预测最佳水平,即碳毡(CF)阳极材料、6 cm2阳极表面、7 g/L葡萄糖浓度和−0.1 V外加电位。实验验证了这些理论结果,最大电流输出为5.23±0.30A/m2,脱色率为100%,化学需氧量(COD)去除率为96±1%。Illumina Miseq结果显示,在门水平上,厚壁菌门(67.1%)占生物节点所在的细菌群落的主导地位。在物种水平上,生物膜主要由Orenia metallireducens物种定植(59.5%)。研究结果表明,THB在降解难降解分子和能量回收方面具有很好的应用前景。
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引用次数: 0
Induction and Characterisation of Lignocellulolytic Activities from Novel Deep-Sea Fungal Secretomes 新型深海真菌分泌体木质纤维素水解活性的诱导与表征
IF 3.7 3区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2023-08-23 DOI: 10.3390/fermentation9090780
B. Dowd, M. Tuohy
Fungi are increasingly recognised as being able to inhabit extreme environments. The deep sea is considered an extreme environment because of its low temperatures, high hydrostatic and lithostatic pressures, 3.5% salinity, and low oxygen, nutrient and light availability. Fungi inhabiting the deep sea may have evolved to produce proteins that allow them to survive these conditions. Investigation and characterisation of fungal lignocellulolytic enzymes from extreme environments like the deep sea is needed, as they may have unusual adaptations that would be useful in industry. This work, therefore, aimed to profile in detail the lignocellulolytic capabilities of fungi isolated from deep-sea sediments in the Atlantic Ocean, and a comparative lignocellulolytic terrestrial isolate. The isolates were strains of Emericellopsis maritima, Penicillium chrysogenum, P. antarcticum and Talaromyces stollii. Lignocellulolytic enzyme induction was achieved using liquid-state fermentation (LSF) with wheat bran as the main carbon source, while enzyme characteristics were evaluated using biochemical assays and gel-based proteomics. This study revealed that the isolates were halotolerant, produced xylanase over wide pH and temperature ranges, and produced a variety of glycoside hydrolase and feruloyl esterase activities. The T. stollii secretome demonstrated remarkable levels of exo-glycoside hydrolase activity, with xylanase activity optimum between pH 1.5–6.0 and temperatures between 1–60 °C, making this isolate an ideal candidate for biotechnological applications. This study is the first to quantitatively characterise xylanase activities and exo-glycoside hydrolase activities secreted by E. maritima, P. antarcticum and a marine T. stollii strain. This study is also the first to quantitatively characterise xylanase activities by a marine strain of P. chrysogenum during LSF.
人们越来越认识到真菌能够在极端环境中生存。深海被认为是一个极端的环境,因为它的低温,高静水和静岩压力,3.5%的盐度,低氧,营养和光的可用性。生活在深海中的真菌可能已经进化到能够产生蛋白质,使它们能够在这些条件下生存。来自深海等极端环境的真菌木质纤维素水解酶的研究和特性是必要的,因为它们可能具有不同寻常的适应性,在工业上是有用的。因此,这项工作旨在详细描述从大西洋深海沉积物中分离的真菌的木质纤维素分解能力,以及比较木质纤维素分解的陆地分离物。分离株为海洋绿绿杆菌、青霉菌、南极青霉和斯托利Talaromyces stollii。以麦麸为主要碳源,通过液相发酵(LSF)诱导木质纤维素水解酶,并通过生化分析和凝胶蛋白组学对酶的特性进行了评价。该研究表明,该菌株耐盐,在较宽的pH和温度范围内产生木聚糖酶,并产生多种糖苷水解酶和阿魏酰酯酶活性。stollii分泌组表现出显著的外糖苷水解酶活性,在pH 1.5-6.0和温度1-60°C之间木聚糖酶活性最佳,使该分离物成为生物技术应用的理想候选者。本研究首次定量测定了E. maritima、P. antarcticum和一个海洋T. stollii菌株分泌的木聚糖酶活性和外糖苷水解酶活性。这项研究也是第一个定量表征海洋菌株在LSF期间的木聚糖酶活性。
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引用次数: 0
Solid-State Fermentation of Green Tea Residues as Substrates for Tannase Production by Aspergillus niger TBG 28A: Optimization of the Culture Conditions 黑曲霉TBG 28A固态发酵绿茶渣生产单宁酶:培养条件的优化
IF 3.7 3区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2023-08-23 DOI: 10.3390/fermentation9090781
Erick M. Peña-Lucio, M. Chávez-González, L. Londoño-Hernandez, Héctor A. Ruiz, J. Martínez-Hernández, M. Govea-Salas, Pradeep Nediyaparambil Sukumaran, Sabu Abdulhameed, Cristóbal N. Aguilar
Tea (Camellia sinensis) is an evergreen shrub that is recognized worldwide for its functional properties. The current global production of green tea is approximately 5.3 million tons per year. Green tea processing has severely affected the generation of agro-industrial waste. One strategy for reducing waste accumulation is the revalorization of agro-industrial wastes via solid-state fermentation (SSF). The aim of this study was to valorize green tea processing residues to produce tannase under SSF using an endemic strain from Western Ghats, Aspergillus niger TBG 28A. SSF was performed in Erlenmeyer flasks with spent green tea leaves inoculated with spores of A. niger TBG 28A. Bioprocess optimization was carried out by employing the Box–Benkhen experimental design, achieving a high enzymatic yield of 246.82 (U/g). The present study shows the complexity of the degradation of tannins and the different patterns of expression of fungal tannase obtained from A. niger TBG 28 A. The enzyme was further purified to obtain a fold purification of 16.35% and a molecular mass of 150 kDa. Producing tannase with a novel strain of A. niger TBG 28A is an interesting strategy to revalorize green tea waste.
茶(Camellia sinensis)是一种常绿灌木,因其功能特性而闻名于世。目前全球绿茶产量约为每年530万吨。绿茶加工严重影响了农业工业废物的产生。减少废物积累的一种策略是通过固态发酵(SSF)对农用工业废物进行重新估价。本研究的目的是使用来自西高止山脉的地方菌株黑曲霉TBG 28A,在SSF下对绿茶加工残留物进行估价,以生产单宁酶。用接种了黑曲霉TBG 28A孢子的用过的绿茶叶在锥形烧瓶中进行SSF。采用Box–Benkhen实验设计进行生物工艺优化,实现了246.82(U/g)的高酶产量。本研究显示了单宁降解的复杂性和从黑曲霉TBG28A中获得的真菌单宁酶的不同表达模式。该酶被进一步纯化,获得16.35%的倍数纯化和150kDa的分子量。用一株新的黑曲霉TBG 28A菌株生产单宁酶是一种有趣的对绿茶废料进行再脱色的策略。
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引用次数: 1
Highly Efficient Biosynthesis of γ-Bisabolene with a New Sesquiterpene Synthase AcTPS5 by Dual Cytoplasmic-Peroxisomal Engineering in Saccharomyces cerevisiae 双胞质-过氧化物酶工程在酿酒酵母中高效合成γ-双abolene的研究
IF 3.7 3区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2023-08-22 DOI: 10.3390/fermentation9090779
Jiajia Liu, G. Yao, Xiukun Wan, Fuli Wang, Penggang Han, Shaoheng Bao, Kang Wang, Tianyu Song, Hui Jiang
γ-bisabolene is a monocyclic sesquiterpene with various biological activities; it has also been approved as a food additive. Additionally, the hydrogenated form of bisabolene is considered as a potential alternative to D2 diesel. Saccharomyces cerevisiae has the ability to produce a large amount of acetyl-CoA in both cytosol and peroxisomes, which serves as a precursor in terpene biosynthesis. In this study, AcTPS5 was identified as a new γ-bisabolene synthase. By expressing AcTPS5 and the mevalonate pathway in peroxisomes, γ-bisabolene titer was achieved at 125.0 mg/L. Deleting the peroxisome autophagy gene atg36 further improved γ-bisabolene production to 216.9 mg/L. The implementation of dual cytoplasmic–peroxisomal engineering further boosted γ-bisabolene production to 296.4 mg/L. Finally, through increasing the acetyl-CoA supply and down-regulating the expression of ERG9, γ-bisabolene production was achieved at 584.14 mg/L in shake-flask fermentation and 2.69 g/L in fed-batch fermentation, which is the highest reported production of γ-bisabolene to date. The strategy presented in this study provides an efficient approach for terpene production in S. cerevisiae.
γ-双abolene是一种具有多种生物活性的单环倍半萜;它也被批准作为食品添加剂。此外,双abolene的氢化形式被认为是D2柴油的潜在替代品。酿酒酵母在细胞质和过氧化物酶体中都能产生大量的乙酰辅酶a,它是萜烯生物合成的前体。在本研究中,AcTPS5被鉴定为新的γ-双abolene合成酶。通过在过氧化物酶体中表达AcTPS5和甲羟戊酸途径,获得了125.0 mg/L的γ-双abolene滴度。删除过氧化物酶体自噬基因atg36进一步提高γ-双abolene产量至216.9 mg/L。双细胞质-过氧化物酶体工程的实施进一步将γ-双abolene的产量提高到296.4 mg/L。最后,通过增加乙酰辅酶a的供应和下调ERG9的表达,摇瓶发酵的γ-双abolene产量为584.14 mg/L,分批补料发酵的产量为2.69 g/L,这是迄今为止报道的γ-双abolene的最高产量。本研究为酿酒葡萄生产萜烯提供了一条有效途径。
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引用次数: 0
Photobioreactor Design for Polyhydroxyalkanoate Production Using Anoxygenic Photoheterotrophs: A Review 利用缺氧光异养菌生产聚羟基烷酸酯的光生物反应器设计:综述
IF 3.7 3区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2023-08-21 DOI: 10.3390/fermentation9080778
Sultan Shaikh, Naim Rashid, Gordon McKay, H. Mackey
This review paper provides an overview of various types of photobioreactors (PBRs) that could be used for the production of polyhydroxyalkanoates (PHAs) using anoxygenic photoheterotrophs, with a focus on the design and operation of these systems. The paper highlights the potential of different PBRs based on reactor geometry and growth mode, and also examines the advantages and disadvantages of each PBR type and summarizes their suitability for PNSB-PHA production. The optimization of reactor design and operation is crucial for maximizing PNSB growth and PHA productivity. The self-immobilization of bacteria in granular sludge is a promising technology for wastewater treatment and the production of PHAs, while grooved-surface PBRs and porous-substrate PBRs have limitations due to difficult biomass harvesting in the former and the presence of aerobic conditions incongruent with PNSB culturing in the latter. Limitations exist with all solutions for maximizing rapid growth and maintaining high biomass concentrations due to the requirements of phototrophic growth.
本文概述了可用于使用缺氧光异养菌生产聚羟基烷酸酯(PHA)的各种类型的光生物反应器(PBRs),重点介绍了这些系统的设计和操作。本文强调了基于反应器几何形状和生长模式的不同PBR的潜力,还考察了每种PBR类型的优缺点,并总结了它们对PNSB-PHA生产的适用性。反应器设计和操作的优化对于最大限度地提高PNSB生长和PHA生产率至关重要。细菌在颗粒污泥中的自固定化是一种很有前途的废水处理和PHA生产技术,而沟槽表面PBRs和多孔基质PBRs由于前者难以收获生物质,以及后者存在与PNSB培养不一致的好氧条件而受到限制。由于光养生长的要求,所有用于最大限度地实现快速生长和保持高生物量浓度的解决方案都存在局限性。
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引用次数: 0
Research on the Promotion of Sludge Anaerobic Fermentation with Sodium Citrate under Low Concentrations of Polyaluminum Chloride 低浓度聚合氯化铝下柠檬酸钠促进污泥厌氧发酵的研究
IF 3.7 3区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2023-08-21 DOI: 10.3390/fermentation9080776
Pu-yi Zhu, Yilin Wang, Hui Bai, Jing Feng, Rui Zhang, Duo Bu, Zeng Dan, Wei Li, Xuebin Lu
Polyaluminum chloride (PAC) is used widely and increasingly in wastewater treatment plants, resulting in its inevitably high production in sludge. Previous studies have indicated that the production of short-chain fatty acids (SCFAs) is inhibited by the existence of PAC in sludge anaerobic fermentation, so it is necessary to study how to promote sludge anaerobic fermentation under low concentrations of PAC. In this study, sodium citrate (SC) was first used to improve the efficiency of anaerobic fermentation under low concentrations of PAC. The results showed that the production of SCFAs increased with SC, especially when the ratio of PAC to SC was 1:2, and the maximum production of SCFAs reached 2890 mg/L, which is 2.5 times more than when PAC only exists. The mechanism studies showed that SC could remove the Al3+ in the sludge floc, which led to deflocculation of the sludge floc, accelerated the destruction of extracellular polymers (EPS), and released soluble substances in the sludge. At the same time, the key enzymes that were bound and hidden originally in the sludge were also released, which promoted the further degradation of organic matter and shortened the anaerobic fermentation period. However, the higher concentration of SC was not conducive to sludge dehydration. Therefore, the optimal distribution ratio of PAC to SC should be 1:1. This study provides a new idea for the research and practical application of sludge anaerobic fermentation.
聚合氯化铝(PAC)在污水处理厂的应用越来越广泛,必然导致其在污泥中的高产量。以往的研究表明,短链脂肪酸(SCFAs)的产生受到污泥厌氧发酵中PAC的抑制,因此有必要研究如何在低浓度PAC下促进污泥厌氧发酵。本研究首先采用柠檬酸钠(SC)来提高低浓度PAC下厌氧发酵的效率,结果表明,SCFAs的产生随着SC的增加而增加;特别是当PAC与SC的比例为1:2时,scfa的最大产量达到2890 mg/L,是单独存在PAC时的2.5倍。机理研究表明,SC能去除污泥絮团中的Al3+,导致污泥絮团脱絮,加速胞外聚合物(EPS)的破坏,并释放污泥中的可溶性物质。同时,原本被束缚和隐藏在污泥中的关键酶也得到了释放,促进了有机物的进一步降解,缩短了厌氧发酵周期。但高浓度的SC不利于污泥脱水。因此,PAC与SC的最佳分配比例应为1:1。本研究为污泥厌氧发酵的研究和实际应用提供了新的思路。
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引用次数: 0
Effects of Different Cutting Stages and Additives on the Fermentation Quality and Microbial Community of Sudangrass (Sorghum sudanense Stapf.) Silages 不同扦插阶段及添加剂对苏丹高粱青贮发酵品质及微生物群落的影响
IF 3.7 3区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2023-08-21 DOI: 10.3390/fermentation9080777
Qiang Yu, Mengxin Li, Yu Zhang, Jinyi Xu, Ping Li, Hong Sun, Yixiao Xie, Rui Dong, Yulong Zheng, Chao Chen
(1) Background: Previous studies have indicated that ferulic acid esterase (FAE), cellulase and xylanase have synergistic effects in lignocellulose degradation, and the cutting stage has a major impact on silages. Whether these additives affect the silages at different cutting stages is unclear. (2) Methods: Sudangrass height at the tested cutting stages was 1.8 m (S1) and 2.0 m (S2). The silage from the two cutting stages was treated with FAE-producing Lactobacillus plantarum (LP), cellulase and xylanase (CX) and a combination of LP and CX (LP+CX) for 30 and 60 days. (3) Results: Compared with CK, adding LP+CX significantly decreased the pH and the content of neutral detergent fiber (NDF) and acidic detergent fiber (ADF) (p < 0.05) and increased the lactic acid (LA) concentration (p < 0.05), dry matter (DM) content and crude protein content. Adding LP+CX effectively degraded lignocellulose in sudangrass, and the NDF and ADF degradation rates at the two stages were all more than 30%. In comparison, cutting at the S2 stage led to a lower pH and higher LA and DM contents (p < 0.05). Additives and the cutting stage exerted a strong effect on the silage microbial community, and Firmicutes and Lactiplantibacillus became the most dominant bacterial phyla and genera, especially at the S2 stage. (4) Conclusions: The results suggest that FAE-producing L. plantarum, cellulase and xylanase had synergistic effects on sudangrass silages, especially at the S2 stage, and their use can thus serve as an efficient method for ensiling.
(1) 背景:以往的研究表明,阿魏酸酯酶(FAE)、纤维素酶和木聚糖酶对木质纤维素的降解具有协同作用,切割阶段对青贮饲料的影响较大。这些添加剂是否会影响不同切割阶段的硅烷尚不清楚。(2) 方法:试验扦插阶段的苏丹草高度分别为1.8m(S1)和2.0m(S2)。用产生FAE的植物乳杆菌(LP)、纤维素酶和木聚糖酶(CX)以及LP和CX的组合(LP+CX)处理来自两个切割阶段的青贮饲料30天和60天。(3) 结果:与对照组相比,添加LP+CX可显著降低pH值、中性洗涤纤维(NDF)和酸性洗涤纤维(ADF)的含量(p<0.05),增加乳酸(LA)浓度(p<0.05)、干物质(DM)含量和粗蛋白含量。添加LP+CX可有效降解苏丹草中的木质纤维素,两个阶段的NDF和ADF降解率均在30%以上。相比之下,S2阶段的切割导致pH较低,LA和DM含量较高(p<0.05)。添加剂和切割阶段对青贮微生物群落产生了强烈的影响,厚壁菌门和乳酸杆菌成为最具优势的细菌门属,尤其是在S2阶段。(4) 结论:FAE生产植物乳杆菌、纤维素酶和木聚糖酶对苏丹草青贮具有协同作用,尤其是在S2阶段,因此它们的使用可以作为一种有效的青贮方法。
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引用次数: 0
Survey on Yeast Assimilable Nitrogen Status of Musts from Native and International Grape Varieties: Effect of Variety and Climate 国内外葡萄品种酵母菌可吸收氮状况的调查:品种和气候的影响
IF 3.7 3区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2023-08-19 DOI: 10.3390/fermentation9080773
E. Bouloumpasi, A. Skendi, Evangelos H. Soufleros
Yeast assimilable nitrogen (YAN), besides the oenological parameters (sugar content, titratable acidity, and pH) in grape musts of sixteen native and international varieties of Vitis vinifera cultivated in six regions of Northern Greece, was assessed in the frame of the present study. Low levels of YAN are frequently thought to be the cause of problematic fermentations and originate significant changes in the organoleptic aspects of the finished product. The objective of this multi-variety study was to assess factors affecting the YAN amount and composition in technologically mature grapes and, therefore, to evaluate the necessity of YAN supplementation with ammonium salts in musts across different native and international grape varieties. Free amino nitrogen was measured colorimetrically, ammoniacal nitrogen was measured enzymatically, and their values for each must sample were summed to obtain the total amount of YAN. Statistical analysis was carried out including principal component analysis (PCA) to discover relationships among must samples and the parameters studied. PCA analysis classified samples depending on grape varieties and region of origin, bringing knowledge about native and international cultivars of great commercial interest. Moreover, these findings could help to understand how commercial varieties can behave in different climates in the climate change context.
在本研究的框架内,评估了希腊北部六个地区种植的16个本地和国际葡萄品种葡萄的酵母可吸收氮(YAN),以及酿酒参数(糖含量,可滴定酸度和pH值)。低水平的YAN通常被认为是发酵问题的原因,并引起成品感官方面的重大变化。这项多品种研究的目的是评估影响技术成熟葡萄中YAN含量和成分的因素,从而评估在不同本地和国际葡萄品种的葡萄汁中添加铵盐YAN的必要性。用比色法测定游离氨基氮,用酶法测定氨态氮,并将它们在每个样品中的值相加,得到YAN的总量。统计分析包括主成分分析(PCA)来发现样本和研究参数之间的关系。PCA分析根据葡萄品种和产地对样品进行分类,从而了解具有重大商业利益的本地和国际品种。此外,这些发现可以帮助理解商业品种在气候变化背景下如何在不同的气候条件下表现。
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引用次数: 0
Design and Construction of a New Reactor for Flexible Biomethanation of Hydrogen 一种新型氢气柔性生物甲烷化反应器的设计与建造
IF 3.7 3区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2023-08-19 DOI: 10.3390/fermentation9080774
K. Hoffstadt, D. Cheenakula, M. Nikolausz, S. Krafft, H. Harms, I. Kuperjans
The increasing share of renewable electricity in the grid drives the need for sufficient storage capacity. Especially for seasonal storage, power-to-gas can be a promising approach. Biologically produced methane from hydrogen produced from surplus electricity can be used to substitute natural gas in the existing infrastructure. Current reactor types are not or are poorly optimized for flexible methanation. Therefore, this work proposes a new reactor type with a plug flow reactor (PFR) design. Simulations in COMSOL Multiphysics ® showed promising properties for operation in laminar flow. An experiment was conducted to support the simulation results and to determine the gas fraction of the novel reactor, which was measured to be 29%. Based on these simulations and experimental results, the reactor was constructed as a 14 m long, 50 mm diameter tube with a meandering orientation. Data processing was established, and a step experiment was performed. In addition, a kLa of 1 h−1 was determined. The results revealed that the experimental outcomes of the type of flow and gas fractions are in line with the theoretical simulation. The new design shows promising properties for flexible methanation and will be tested.
可再生电力在电网中所占份额的增加推动了对足够存储容量的需求。特别是对于季节性储存,电力转天然气可能是一种很有前途的方法。从剩余电力中产生的氢气中生物产生的甲烷可以用来替代现有基础设施中的天然气。目前的反应器类型没有或很差地针对柔性甲烷化进行优化。因此,本工作提出了一种新型的塞流反应器(PFR)设计。COMSOL Multiphysics®的模拟显示出在层流中运行的良好性能。进行了一项实验来支持模拟结果,并确定新型反应器的气体分数,测量结果为29%。基于这些模拟和实验结果,反应器被构造为具有曲折方向的14m长、50mm直径的管。建立了数据处理方法,并进行了分步实验。此外,确定了1 h−1的kLa。结果表明,该类型的流量和气体分数的实验结果与理论模拟一致。新的设计显示出柔性甲烷化的良好性能,并将进行测试。
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Fermentation-Basel
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