Dtw. Deutsche Tierärztliche Wochenschrift最新文献

Neoplastic diseases were described very rarely in addax (Addax nasomaculatus). In this communication clinical signs, morphological and immunohistological findings in a 15-year-old, female addax with a primary cutaneous, re-occurring and metastasing haemangiosarcoma of a forelimb are reported.

The aim of this study was to evaluate the polymerase chain reaction (PCR) assay for detection of Brucella melitensis in stomach content samples of aborted sheep fetuses and to compare its performance with bacteriological culture method. It was also aimed to determine the agreement between PCR and Rose Bengal plate test (RBPT). Materials were collected from aborted sheep from 109 different sheep flocks in the region of Van during the lambing seasons of 2004-2005 and 2005-2006. Stomach contents from 135 aborted sheep fetuses were examined by bacteriological culture and PCR, and 135 sera from these aborting ewes were tested by RBPT. Identification and typing of Brucella strains were performed using standard classification test. B. melitensis biovar 3 was isolated from 26 (19.2%) of foetal stomach contents. B. melitensis was detected by PCR in 29 (21.4%) stomach content samples. Twenty five sera (18.5%) from aborting ewes tested positive by RBPT. The detection limit of B. melitensis 16 M strain by PCR was 1.7 x 10(3) cfu (colony forming units) /ml in spiked stomach contents. Diagnostic sensitivity and specificity of the PCR were detected as 100% and 97.2%, respectively. The agreement between PCR and RBPT was found to be 97%. In conclusion, PCR assay would have an advantage over conventional bacteriological culture method, but in particular for its ability to meet the specificity requirements for the detection of B. melitensis in stomach content samples of aborted sheep fetuses.

Since the amendment of the animal welfare law in Germany in April 2006, castration of piglets without anesthesia is only allowed in the first seven days of life. However, neonates are capable of feeling pain and react more sensitive to pain than adults. Accordingly we expect that castration without anesthesia will be unaccepted in Germany in the future as castration without anesthesia will be forbidden in Switzerland from 2009 on. Denmark and The Netherlands strive for an embargo of the castration without anesthesia and Norway is already asking for an elimination of pain during castration. In Switzerland castration under isoflurane-anesthesia is considered to be a good alternative for the castration without anesthesia. Whether isoflurane-anesthesia is able to reduce stress caused by castration adequately or anesthesia, especially anesthetization that often provokes defensive reaction, is stress for the animal itself was tested in the following investigation. To get an objective statement about the stress of the animals, concentrations of epinephrine and norepinephrine were detected before and immediately after castration/fixation with or without anesthesia. Five day old, healthy animals were divided into four groups. Animals of group A (n = 10) were only fixed without anesthesia, animals of group B (n = 10) were fixed and castrated without anesthesia. In group C (n = 10) the animals were only fixed under anesthesia and the animals of group D (n = 11) were fixed and castrated under anesthesia. Norepinephrine-concentrations and epinephrine-concentrations did rise significantly in all piglets handled without anesthesia (group A and B) independently if castrated or only handled. The values of these animals were significantly higher than the values of the anesthetized animals after fixation/castration (group C and D). In contrast the norepinephrine-concentrations and the epinephrine-concentrations of all anesthetized animals (group C and D) were significantly lower after castration than the concentrations before castration. The catecholamine peak did not differ significantly between the groups of anesthetized animals. The significant increase of the catecholamines of the non-anesthetized animals can be explained by the stress caused by fixation and/or castration without anesthesia. Because of the anesthesia the catecholamine-concentration was reduced considerably during the fixation and/or castration. The results show that fixation and/or castration under anesthesia causes no stress for the piglets.

A ventral meningomyelocele was found in a five week old, male German warmblood foal which exhibited central nervous symptoms. This rare malformation in horses may be caused by absent or defective fusion of vertebral bodies leading to herniation of the spinal cord and meninges with subsequent degenerative alterations of the spinal cord.

The present investigation was undertaken to assess the effects of aflatoxin (AF) containing diets on alpha and beta cells of the endocrine pancreas in young quails by means of light and electron microscopy. A total of thirty quails were divided into 3 groups, each comprising 10 animals. Total AF was incorporated into the diet of these groups, at dosages of 0 (control, group 1), 2.5 (group 2), and 5.0 (group 3) mg AF/kg feed. The chicks were housed in electrically heated battery cages and exposed to light for 24 h from hatching to 3 weeks of age. Quails consumed the diets and water ad libitum. Electron microscopic examinations demonstrated degranulation of alpha cells, decrease in the size and number of secreting granules, and increase in the number of free ribosomes and polisomes in the animals of group 2 and 3. In beta cells, the numbers of free ribosomes and polisomes decreased, whereas the number of mature granules increased in the animals of group 3. Mononuclear cell infiltrates were observed in the periphery of capillaries and around endocrine islets in the experimental groups. Furthermore, capillaries of the animals in group 2 and 3 were dilated at all sides of both alpha and beta islets. According to the results of this study, the addition of aflatoxin to the diets of quails at dosage of 2.5 and 5 mg AF/kg leads to significant changes in pancreatic alpha and beta cells. These changes may exhibit adverse effect on the metabolism of carbohydrates in poultry.

Cutaneous amyloidosis occurs as primary localized form or secondary to systemic amyloidosis. In dogs, cutaneous amyloidosis is reported in association with localized plasma cell proliferation or cutaneous extramedullary plasmacytoma. Primary cutaneous amyloidosis is a very rare condition in dogs. There is paucity of information and published report on primary nodular cutaneous amyloidosis in dogs. This report describes a case of primary nodular cutaneous amyloidosis for the first time in a male Golden Retriever.

This literature review gives information about important behaviour categories of laying hens kept in aviary systems. Based on current knowledge, the differences in behaviour of hens in aviaries compared to the behaviour of hens living under "close to natural" conditions are assessed quantitatively and qualitatively. The focus of this first review is put on resting and particularly on social behaviour. So far "optimal" group size for laying hens and consequences of oversized groups for the well-being of laying hens are unknown, thus, rendering further research necessary. Referring to the resting and social behaviour of laying hens, proposals for the design of the housing system aviary are given. A second part will deal with feeding, reproductive and dustbathing behaviour.

The aim of this study was to evaluate the efficacy of lyophilised C-strain vaccine in domestic pigs and wild boar after oral application. A new spherical bait form (diameter 3 cm) containing lyophilised vaccine virus and the recent vaccine baits were used for animal experiments. Four vaccination groups were established in experiment 1 (group 1: recent liquid bait vaccine; group 2: spherical baits containing one dose of the lyophilised vaccine; groups 3 (domestic pigs) and 4 (wild boar): spherical baits containing two doses of the lyophilised vaccine) and two groups in experiment 2 (group 1: recent liquid bait vaccine; group 2: spherical baits with two doses of the lyophilised vaccine). Challenge was carried out with the highly virulent virus strain "Alfort 187" (using 100 TCID50 in the first and 1.000 TCID50 in the second experiment). Our results showed that the animals vaccinated with lyophilised C-strain vaccine developed high neutralising antibody titres comparable to those obtained after vaccination with the recent bait vaccine. All pigs which picked up the baits remained healthy after challenge. Neither clinical symptoms nor viremia or virus shedding were observed after infection except in one pig (group 2, experiment 2) which had not consumed the vaccine bait. The surviving domestic pigs and wild boar were tested negative for CSFV and viral RNA at the end of the study. This result demonstrates that lyophilised vaccine may become an effective vaccine formulation for oral immunisation of wild boar against CSF in the near future.

Navicular disease or podotrochlosis has long been known to cause forelimb lameness in horses. It had been proposed that the development of podotrochlosis has similarities to the human osteoarthritis (OA) complex. Alterations of the navicular bone can be made visible early in life only on the basis of radiographs. Reports on the prevalences of navicular disease indicate that radiological alterations in the navicular bone are present in different warmblood populations at frequencies of between 14.9% and 87.6%. Genetic factors play an important role in the development of the radiological signs. Estimates of heritability using animal threshold models range from h2 = 0.09 to h2 = 0.40. Estimated additive genetic correlations between radiological changes in the navicular bone and other orthopaedic health traits indicated that they mostly develop genetically independently of each other. There was a negative genetic correlation between radiological changes in the navicular bone and the number of tournament entries and placings. It has also been shown that reduction of radiological changes of navicular bones and improvement of breeding values for performance of riding horses can be achieved if selection is based on breeding values for these traits simultaneously. An optimised markerset was developed to detect quantitative trait loci (QTL) for pathologic changes in the navicular bone of Hanoverian warmblood horses. The horse genome was scanned using 214 highly polymorphic microsatellites chromosome-wide significant QTL were located on equine chromosomes (ECA) 2, 3, 4, 10, and 26. Genome-wide significant QTL were on ECA2 and on ECA10. Unravelling QTL associated with navicular disease will enhance selection progress for a healthy limb constitution in horses.