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The Forensic Isotope Ratio Mass Spectrometry (FIRMS) network Good Practice Guide for Isotope Ratio Mass spectrometry 3rd edition 法医同位素比质谱(FIRMS)网络同位素比质谱良好实践指南第3版
IF 2 4区 医学 Q2 MEDICINE, LEGAL Pub Date : 2025-10-15 DOI: 10.1016/j.scijus.2025.101345
Philip J.H. Dunn , Christopher Brodie , Ethan Strak , James F. Carter
The Forensic Isotope Ratio Mass Spectrometry (FIRMS) Network announce the publication of the Third Edition of the Good Practice Guide for Isotope Ratio Mass Spectrometry. In the third edition, changes include a new chapter on sampling covering method of sampling a population, sampling for homogeneity testing of a material as well as considerations for measurement uncertainty arising from sampling. The measurement uncertainty section has been revised and expanded to touch on alternative methods to combine uncertainty components together. Methods for measurement of water isotopic composition using equilibration have also been added. Additional detail has been included to expand on topics such as the difference between a reference gas and a working gas, additional tests that might be performed to check instrumental performance and initial data processing such as peak identification amongst others.
法医同位素比质谱(FIRMS)网络宣布出版《同位素比质谱良好实践指南》第三版。在第三版中,变化包括一个关于抽样的新章节,涵盖抽样总体的方法,材料的均匀性测试的抽样以及由抽样引起的测量不确定度的考虑。测量不确定度部分已被修订和扩展,以触及将不确定度分量组合在一起的替代方法。还增加了用平衡法测量水同位素组成的方法。额外的细节包括扩展主题,如参考气体和工作气体之间的差异,可能执行的额外测试,以检查仪器性能和初始数据处理,如峰识别等。
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引用次数: 0
Artificial intelligence-assisted estimation of postmortem intervals in bacterially infected cadavers using pathological imaging across variable temperature conditions 人工智能辅助估计细菌感染尸体在不同温度条件下使用病理成像的死后间隔
IF 2 4区 医学 Q2 MEDICINE, LEGAL Pub Date : 2025-10-12 DOI: 10.1016/j.scijus.2025.101344
Xinggong Liang , Gongji Wang , Han Wang , Zhengyang Zhu , Wanqing Zhang , Yuqian Li , Jianliang Luo , Shuo Wu , Run Chen , Mingyan Deng , Hao Wu , Chen Shen , Gengwang Hu , Kai Zhang , Qinru Sun , Zhenyuan Wang
Accurate estimation of the postmortem interval (PMI) is crucial in forensic investigations but remains challenging due to environmental, individual, and cause-of-death variables. Traditional methods relying on postmortem changes (e.g., livor mortis, rigor mortis) are subjective and limited, especially for late PMI. Recent advances in artificial intelligence (AI) and computational pathology, particularly whole-slide imaging (WSI), enable data-driven PMI estimation by analyzing digital pathology images with high precision and reproducibility. Building on previous successes in using AI to estimate PMI in uninfected conditions, this study extends the method to bacterially infected mouse cadavers (Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa) under varying temperatures (25 °C, 37 °C, and 4 °C). The results demonstrate the model’s robustness in diverse scenarios, achieving micro- and macro-area under the curve values (AUCs) of at least 0.873 (patch-level) and 0.717 (WSI-level) in training and testing sets, and no less than 0.948 (patch-level) in external validation. By leveraging easily prepared pathological sections and AI algorithms, this approach offers a practical, objective, and scalable solution for PMI estimation, enhancing forensic workflows. The integration of computational pathology with forensic science establishes a new technical benchmark for PMI estimation in both infected and uninfected cases.
准确估计死亡间隔(PMI)在法医调查中至关重要,但由于环境、个人和死因等因素,仍然具有挑战性。传统的方法依赖于尸体的变化(例如,尸体的livor mortis,僵硬性mortis)是主观的和有限的,特别是对于晚期PMI。人工智能(AI)和计算病理学的最新进展,特别是全切片成像(WSI),通过分析具有高精度和可重复性的数字病理图像,实现数据驱动的PMI估计。基于先前在未感染条件下使用人工智能估算PMI的成功,本研究将该方法扩展到不同温度(25°C, 37°C和4°C)下细菌感染的小鼠尸体(金黄色葡萄球菌,大肠杆菌和铜绿假单胞菌)。结果表明,该模型在不同场景下具有较强的稳健性,在训练集和测试集上,曲线下的微观和宏观面积(auc)至少为0.873 (patch-level)和0.717 (WSI-level),在外部验证中,auc不小于0.948 (patch-level)。通过利用易于准备的病理切片和人工智能算法,该方法为PMI估计提供了实用、客观和可扩展的解决方案,从而增强了法医工作流程。计算病理学与法医科学的结合为感染和未感染病例的PMI估计建立了新的技术基准。
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引用次数: 0
Effects of ultraviolet exposure on latent fingermark visualization on expanded polystyrene surfaces 紫外曝光对聚苯乙烯膨胀表面手印显现的影响
IF 2 4区 医学 Q2 MEDICINE, LEGAL Pub Date : 2025-10-04 DOI: 10.1016/j.scijus.2025.101343
Bok-Chan Go , Jiwon Shin , Sungwook Hong
It is known that the physical and chemical properties of expanded polystyrene (EPS) surfaces are altered upon exposure to ultraviolet (UV) light; however, the implications for latent fingermark visualization have not been fully explored. In this study, EPS surfaces were exposed to UV light (253.7 nm) either before or after fingermark deposition and subsequently treated with five visualization techniques: Dazzle yellow, cyanoacrylate fuming (CA) followed by fluorescent dye staining, Lumicyano, small particle reagent (SPR), and Nile red. When illuminated with a blue or green forensic light source, photoluminescence was observed exclusively on UV-exposed surfaces, allowing clear differentiation between UV-exposed and unexposed EPS. Experimental results indicated that Nile red produced the highest-quality fingermarks on unexposed EPS surfaces, while Dazzle yellow and Nile red were most effective for UV-exposed surfaces. In contrast, CA followed by rhodamine 6G staining (CA/R6G) and Lumicyano methods exhibited markedly reduced performance on UV-exposed EPS due to photochemically induced surface alterations. These findings highlight the importance of assessing UV exposure prior to fingermark development and suggest appropriate visualization methods based on surface condition.
众所周知,膨胀聚苯乙烯(EPS)表面的物理和化学性质在暴露于紫外线(UV)光时发生改变;然而,对潜在手印可视化的影响尚未得到充分的探讨。在本研究中,在手印沉积之前或之后,将EPS表面暴露在紫外线(253.7 nm)下,随后使用五种可视化技术进行处理:炫黄,氰基丙烯酸酯熏烟(CA),荧光染料染色,Lumicyano,小颗粒试剂(SPR)和尼罗红。当用蓝色或绿色法医光源照射时,光致发光仅在暴露在紫外线下的表面上观察到,从而明确区分暴露在紫外线下和未暴露的EPS。实验结果表明,尼罗红在未暴露的EPS表面上产生的手印质量最高,而眩黄和尼罗红在紫外线暴露的表面上产生的手印质量最高。相比之下,CA之后的罗丹明6G染色(CA/R6G)和Lumicyano方法由于光化学诱导的表面改变,在紫外线暴露的EPS上表现出明显降低的性能。这些发现强调了在手印形成之前评估紫外线暴露的重要性,并提出了基于表面状况的适当可视化方法。
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引用次数: 0
Letter to the editor: “A preliminary survey of ethnic diversity and experience of discrimination in forensic science in the UK” 致编辑的信:“对英国法医学中种族多样性和歧视经验的初步调查”
IF 2 4区 医学 Q2 MEDICINE, LEGAL Pub Date : 2025-10-04 DOI: 10.1016/j.scijus.2025.101340
Karl Ebejer
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引用次数: 0
Competing explanations in Bayesian networks — The small town murder problem revisited 贝叶斯网络中相互矛盾的解释——小镇谋杀问题的重访
IF 2 4区 医学 Q2 MEDICINE, LEGAL Pub Date : 2025-10-03 DOI: 10.1016/j.scijus.2025.101337
Arvid Sjölander , Gustav Lindkvist
The use of probability theory and statistical reasoning in legal contexts is controversial, with several well-known thought experiments highlighting perceived paradoxes. One example is the small town murder problem, recently discussed by de Zoete et al., who argued that the perceived paradoxes arise from a failure to properly distinguish between hypotheses and evidence, and can be resolved using Bayesian networks. While we agree with their general conclusions, we contend that their analysis of the small town murder problem overlooks a central issue and underutilizes the full potential of Bayesian modeling. In this note, we propose an alternative analysis of the problem, drawing on well-known results on conditioning on a common effect of two factors.
概率论和统计推理在法律环境中的使用是有争议的,有几个著名的思想实验突出了感知的悖论。一个例子是小镇谋杀问题,最近由de Zoete等人讨论,他们认为感知的悖论源于未能正确区分假设和证据,并且可以使用贝叶斯网络来解决。虽然我们同意他们的总体结论,但我们认为他们对小镇谋杀问题的分析忽略了一个核心问题,并且没有充分利用贝叶斯模型的全部潜力。在这篇文章中,我们提出了另一种分析方法,利用众所周知的两个因素共同作用下的条件作用的结果。
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引用次数: 0
Forensic significance of condom traces in sexual assault investigations: A systematic review 性侵调查中避孕套痕迹的法医意义:系统回顾
IF 2 4区 医学 Q2 MEDICINE, LEGAL Pub Date : 2025-10-01 DOI: 10.1016/j.scijus.2025.101341
Saule A. Mussabekova , Nunzianda Frascione , Laura B. Assylbayeva
This systematic review examines the forensic value of condom residue detection in biological samples, particularly in sexual assault investigations where DNA evidence is absent. Following PRISMA 2020 guidelines, a comprehensive literature search was conducted across seven databases (MEDLINE, EMBASE, Cochrane Library, PubMed, Web of Science, SCOPUS, Google Scholar), two trial registers (ClinicalTrials.gov, WHO ICTRP), and four grey literature sources (PQDT, WorldCat, OATD, F1000). Studies published in English from January 1, 2020, to February 28, 2025, were screened using predefined PICO(S) criteria. Eight eligible studies involving human matrices were included. The analytical techniques assessed included DRIFTS-FTIR, ATR-FTIR, Py-GC/MS, GC–MS, and sfPESI-MS. DRIFTS-FTIR demonstrated strong spectral resolution for detecting PDMS-based silicone lubricants, while ATR-FTIR achieved 100 % classification accuracy under controlled laboratory conditions. The combination of spectroscopic and chromatographic techniques (e.g., ATR-FTIR + GC–MS) yielded more reliable and confirmatory results. The risk of bias was assessed using the QUADAS-2 tool for experimental studies and the Joanna Briggs Institute checklist for case reports. Key limitations included small sample sizes, variability in collection protocols, and the lack of validated field methods. Overall, the findings highlight the need for methodological integration and standardisation in forensic lubricant trace analysis. PROSPERO registration: CRD420251004301. No external funding was received.
本系统综述探讨了生物样本中避孕套残留检测的法医价值,特别是在缺乏DNA证据的性侵犯调查中。按照PRISMA 2020指南,对7个数据库(MEDLINE、EMBASE、Cochrane Library、PubMed、Web of Science、SCOPUS、谷歌Scholar)、2个试验注册库(ClinicalTrials.gov、WHO ICTRP)和4个灰色文献来源(PQDT、WorldCat、oad、F1000)进行了全面的文献检索。从2020年1月1日至2025年2月28日以英文发表的研究,使用预定义的PICO(S)标准进行筛选。纳入了8项涉及人体基质的符合条件的研究。评估的分析技术包括drift - ftir、ATR-FTIR、Py-GC/MS、GC-MS和sfPESI-MS。drift - ftir显示出很强的光谱分辨率,用于检测pdm基硅树脂润滑剂,而ATR-FTIR在受控的实验室条件下实现了100%的分类精度。光谱和色谱技术的结合(例如,ATR-FTIR + GC-MS)产生了更可靠和验证的结果。使用QUADAS-2实验研究工具和乔安娜布里格斯研究所病例报告检查表评估偏倚风险。主要的限制包括样本量小、收集方案的可变性以及缺乏经过验证的现场方法。总的来说,研究结果强调了法医润滑油痕量分析方法整合和标准化的必要性。普洛斯彼罗注册号:CRD420251004301。没有收到外部资金。
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引用次数: 0
Current state and barriers to global adoption of forensic evaluative reporting of findings given activity-level propositions 全球采用基于活动层面主张的调查结果法医评估报告的现状和障碍
IF 2 4区 医学 Q2 MEDICINE, LEGAL Pub Date : 2025-09-23 DOI: 10.1016/j.scijus.2025.101336
Janet Stacey , Stephanie Opperman , James M. Curran , Sally Ann Harbison
This article examines the current state and challenges surrounding the global adoption of evaluative reporting of forensic findings given activity-level propositions. Evaluative reporting provides a structured and objective assessment of findings that can be used in judicial proceedings. The assessment of findings given activity-level propositions addresses ‘how’ and ‘when’ questions about the presence of forensic evidence. This is often the question of interest to the fact-finder. Practitioners are finding that they face such questions on the witness stand with increasing frequency. Despite its importance, widespread adoption has been hampered by several barriers. This article explores various concerns raised by the forensic community in different jurisdictions. This includes reticence toward suggested methodologies, concern about a lack of robust and impartial data to inform probabilities, regional differences in regulatory frameworks and methodology, and the availability of training and resources to implement evaluations given activity-level propositions. Suggestions are made for overcoming these barriers and fostering greater global integration of activity-level evaluative reporting. By addressing these barriers, the credibility and utility of forensic evaluations (of evidence considering activity-level propositions) in both legal and investigative contexts can be improved internationally.
本文考察了目前的状态和挑战,围绕全球采用评估报告的法医发现给定的活动水平的主张。评估性报告对可用于司法程序的调查结果进行结构化和客观的评估。对调查结果的评估给出了活动级命题,解决了关于法医证据存在的“如何”和“何时”问题。这通常是事实发现者感兴趣的问题。律师们发现,他们在证人席上面临这样的问题越来越频繁。尽管它很重要,但它的广泛采用受到了一些障碍的阻碍。本文探讨了法医学界在不同司法管辖区提出的各种问题。这包括对建议的方法保持沉默,对缺乏可靠和公正的数据来告知概率的担忧,监管框架和方法的区域差异,以及实施给定活动级主张的评估的培训和资源的可用性。提出了克服这些障碍和促进活动一级评价报告在全球更大程度一体化的建议。通过解决这些障碍,可以在国际上提高法律和调查背景下(考虑到活动层面主张的证据)法医评估的可信度和效用。
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引用次数: 0
Extended reality in forensic sciences: An integrative review 法医学中的扩展现实:综合综述
IF 2 4区 医学 Q2 MEDICINE, LEGAL Pub Date : 2025-09-21 DOI: 10.1016/j.scijus.2025.101339
Michael Joseph Dino , Patrick Tracy Balbin , Chloe Margalaux R. Villafuerte , Mayelle S. Magat-Pangilinan , John David , Princess Alyssa Tee , Lothes Samante , Vincent Acena , Michael Salinas , Emily Daquioag , Banjelyn Lazaro
The use of extended reality (XR), an umbrella term encompassing alternative reality technologies such as augmented reality, virtual reality, and mixed reality, has become increasingly prevalent in various fields of literature over the last few decades. However, there is a limited understanding of XR utilization and integration in forensic science. This integrative review synthesized existing evidence on how XR is being utilized in forensic science. We performed literature searches in four databases and identified 21 articles published between 2010 and 2023 for final analysis. Bibliometric information indicates that most publications (81 %) were published between 2019 and 2023, predominantly in European regions (67 %), in non-forensic journals. Methodologically, qualitative approaches (67 %) were common in evaluating XR usability. Its applications are clustered into either practice (57 %) or education (43 %) domains. Virtual reality (67 %) was more prevalent in XR environments than augmented or mixed realities. Head-mounted devices are the main XR system used in forensic science. While XR offers promising practical and educational applications in forensic science, these findings underscore the need for more robust evidence and the development of transparent, repeatable, and standardized protocols that align with decision-making and accountability in forensic science.
扩展现实(XR)是一个涵盖了增强现实、虚拟现实和混合现实等替代现实技术的总称,在过去几十年里,它在各个文学领域变得越来越普遍。然而,法医科学对XR的利用和整合的了解有限。这篇综合综述综合了关于如何在法医科学中利用x光成像的现有证据。我们在四个数据库中进行文献检索,并确定了2010年至2023年间发表的21篇文章进行最终分析。文献计量学信息表明,大多数出版物(81%)发表于2019年至2023年之间,主要发表在欧洲地区(67%)的非法医期刊上。在方法学上,定性方法(67%)在评估XR可用性中很常见。它的应用集中在实践(57%)或教育(43%)领域。虚拟现实(67%)在XR环境中比增强现实或混合现实更普遍。头戴式设备是法医科学中使用的主要XR系统。虽然XR在法医学中提供了有前景的实践和教育应用,但这些发现强调了需要更有力的证据,并需要制定透明、可重复和标准化的协议,以与法医学的决策和问责制保持一致。
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引用次数: 0
Firearm projectile collection techniques: A comparative study on the retention of textile fibers adhered during projectile perforation of denim fabric 枪械弹丸回收技术:牛仔布弹丸穿孔过程中粘附的纺织纤维保留率的对比研究
IF 2 4区 医学 Q2 MEDICINE, LEGAL Pub Date : 2025-09-18 DOI: 10.1016/j.scijus.2025.101338
Carlos Antonio Vicentin Junior , Raíssa Bastos Vieira , Pércio Almeida Fistarol Filho , Lehi Sudy dos Santos , Melina Calmon Silva , Michele Avila dos Santos , Carlos Eduardo Palhares Machado
Trace evidence plays a critical role in forensic reconstructions, especially when involving microscopic materials transferred during projectile impacts. This study investigated the adherence of blue jeans fibers to 9 mm caliber projectiles, evaluating six projectile collection methods—PVC tube filled with cotton, PVC tube filled with cotton waste, water tank, ballistic vest plates (Kevlar®), combination of cardboard and ballistic plates, and motorcycle tire combined with ballistic plates—and comparing two ammunition types: hollow-point (HP) and full metal jacket (FMJ). Digital microscopy enabled high-resolution, non-destructive analysis of fiber adherence. HP projectiles retained blue jeans fibers in 94.4 % of cases, significantly more than FMJ projectiles (22.2 %), with a highly significant association. Fiber presence was most frequently observed at the projectile tip and within the HP cavity. Barriers using cotton provided optimal conditions for fiber preservation and minimal visual contamination, whereas water tanks caused fragmentation in HP projectiles and the other materials resulted in considerable deformation and visual contamination, hindering analysis. These findings confirm the superior capacity of HP projectiles to retain microtraces and establish cotton-filled PVC tubes as an effective method for ballistic evidence recovery. Therefore, the use of hollow-point projectiles combined with the cotton-filled PVC tube collection technique is recommended for ballistic tests involving textile microtrace analysis. The study also emphasizes that the absence of visible fibers should not be interpreted as a lack of fabric interaction.
微量证据在法医重建中起着至关重要的作用,特别是当涉及弹丸撞击过程中转移的微观物质时。本研究研究了蓝色牛仔裤纤维与9毫米口径弹丸的粘附性,评估了六种弹丸收集方法——填充棉花的PVC管、填充棉花废物的PVC管、水箱、防弹背心板(凯夫拉®)、纸板和防弹板的组合以及摩托车轮胎与防弹板的组合——并比较了两种弹药类型:空心点(HP)和全金属护套(FMJ)。数码显微镜使高分辨率、非破坏性的纤维粘附分析成为可能。HP弹丸在94.4%的病例中保留了蓝色牛仔裤纤维,显著高于FMJ弹丸(22.2%),具有高度显著的相关性。纤维的存在最常被观察到在弹丸尖端和HP腔内。使用棉花的屏障为纤维保存和最小的视觉污染提供了最佳条件,而水箱导致HP弹丸破碎,其他材料导致相当大的变形和视觉污染,阻碍了分析。这些发现证实了高压弹丸保留微量痕迹的优越能力,并确立了棉填充PVC管作为弹道证据恢复的有效方法。因此,建议在涉及纺织品微迹分析的弹道试验中使用空心弹丸结合棉填充PVC管收集技术。该研究还强调,没有可见纤维不应被解释为缺乏织物相互作用。
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引用次数: 0
Advanced deep learning for automatic classification of fired bullets from standard-issue firearms 先进的深度学习自动分类从标准发行的枪支发射的子弹
IF 2 4区 医学 Q2 MEDICINE, LEGAL Pub Date : 2025-09-12 DOI: 10.1016/j.scijus.2025.101335
Bai-En Guo , Yao Shen , Zhi-Fei Zhou , Xing Liu , Yu-Xin Wei , Lei Yang
Gun violence continues to be a significant global issue, causing countless innocent lives to be lost each year. This study explores deep learning for automated fired bullet marking classification. To address inconsistent results from examiners’ subjective same-source judgment, we automate classification to boost forensic firearm examination accuracy and reduce subjectivity. We collected a dataset of 6000 fired bullets from six types of standard-issue firearms commonly used by Chinese law enforcement agencies. Panoramic images of the lateral surfaces of the bullets were captured using the BalScan system. To create diverse and informative inputs for our deep learning model, we employed three distinct image preprocessing strategies: panoramic imaging, land engraved area (LEA) segmentation (the area with striations and grooves created by the gun barrel’s rifling), and line segmentation. Then, we fine-tuned the advanced pre-trained ResNet50 network on this dataset, specifically designed for image classification tasks. Our experiments demonstrated the effectiveness of our approach, achieving high classification accuracy across different firearm types. Notably, the LEA segmentation strategy outperformed the other methods, highlighting the importance of focusing on specific regions of interest for accurate classification. To specify, our algorithm with the LEA segmentation strategy achieves a classification accuracy of 97.2% for six types of firearms with highly similar bullet rifling marks, while attaining 100.0% accuracy for firearms exhibiting significant differences in rifling characteristics, demonstrating a clear superiority over other algorithms. This study paves the way for further research and development in the field of forensic firearm examination through AI-driven solutions, aiming to improve the efficiency and accuracy of firearm identification and investigation processes.
枪支暴力仍然是一个重大的全球问题,每年造成无数无辜生命的丧失。本研究探索了基于深度学习的自动发射子弹标记分类。为了解决审查员主观同源判断结果不一致的问题,我们实现了自动分类,以提高法医枪支检查的准确性并降低主观性。我们收集了6000发发射子弹的数据集,这些子弹来自中国执法机构常用的六种标准枪支。使用BalScan系统捕获了子弹侧面的全景图像。为了为我们的深度学习模型创建多样化和信息丰富的输入,我们采用了三种不同的图像预处理策略:全景成像、陆地雕刻区域(LEA)分割(枪管膛线产生的条纹和凹槽区域)和直线分割。然后,我们在这个数据集上微调高级预训练的ResNet50网络,专门为图像分类任务设计。我们的实验证明了我们的方法的有效性,在不同的枪支类型中实现了很高的分类精度。值得注意的是,LEA分割策略优于其他方法,突出了关注特定感兴趣区域对准确分类的重要性。具体来说,我们采用LEA分割策略的算法,对于子弹膛线痕迹高度相似的6种枪械,分类准确率达到97.2%,对于膛线特征差异较大的枪械,分类准确率达到100.0%,明显优于其他算法。这项研究为通过人工智能驱动的解决方案在法医枪支检查领域的进一步研究和发展铺平了道路,旨在提高枪支识别和调查过程的效率和准确性。
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引用次数: 0
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