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Assessing the influence of variables on vehicle speed determination through reverse projection analysis 通过逆向投影分析评估变量对车速确定的影响
IF 2 4区 医学 Q2 MEDICINE, LEGAL Pub Date : 2025-12-03 DOI: 10.1016/j.scijus.2025.101369
Walter E Bruehs , Dorothy Stout
Reverse projection photogrammetry has long been established as a forensic tool for estimating height and, more recently, vehicle speed from video. Previous work has demonstrated its feasibility under controlled daytime conditions using digital video recorders (DVRs). This study extends that foundation by systematically evaluating how additional variables specifically headlight bloom as a marker, and frame rate variability in network video recorders (NVRs) and cloud-based consumer systems might affect the accuracy of speed determination. Vehicle speeds were independently documented using Light Detection and Ranging (LIDAR) and in-dash speedometer recordings, providing complementary ground truth: LIDAR offered precise point-in-time measures, while the speedometer delivered a continuous average across the test interval. Together, these methods established a baseline for comparison against photogrammetric calculations.
Structured experiments were conducted at a closed test track, with multiple DVR, NVR, and cloud devices recording daytime and nighttime runs at varying speeds. Speed was estimated by measuring vehicle position (or headlight bloom at night) via reverse projection photogrammetry and calculating elapsed time using frame counts or, when necessary, frame analysis tools. Results showed that higher and more stable frame rates reduced uncertainty, while consumer cloud systems and some NVRs introduced dropped or inconsistent frames, producing errors outside the expected margin. Conversely, headlight bloom proved to be a reliable nighttime surrogate for vehicle position.
These findings demonstrate that reverse projection photogrammetry provides a reliable means of estimating vehicle speed when recorder timing is properly accounted for and understood. By quantifying sources of uncertainty across diverse recording systems and lighting conditions, this research strengthens the evidentiary basis for using video-derived speed estimates in forensic analysis and supports their defensible application in casework.
反向投影摄影测量法长期以来一直是一种法医工具,用于估计高度,最近,从视频中估计车辆速度。之前的工作已经证明了它在白天受控条件下使用数字视频录像机(dvr)的可行性。本研究通过系统地评估附加变量(特别是作为标记的前灯绽放)以及网络视频录像机(nvr)和基于云的消费者系统中的帧速率可变性如何影响速度确定的准确性来扩展该基础。使用光探测和测距(LIDAR)和仪表盘内的速度计记录独立记录车辆速度,提供互补的地面事实:LIDAR提供精确的时间点测量,而速度计提供整个测试间隔内的连续平均值。总之,这些方法建立了与摄影测量计算比较的基线。结构化实验在封闭的测试轨道上进行,使用多个DVR、NVR和云设备记录白天和夜间不同速度的运行情况。通过反向投影摄影测量测量车辆位置(或夜间前灯绽放)来估计速度,并使用帧计数或必要时使用帧分析工具计算经过的时间。结果表明,更高和更稳定的帧率减少了不确定性,而消费者云系统和一些nvr引入了丢失或不一致的帧,产生超出预期范围的错误。相反,前灯绽放被证明是一个可靠的夜间替代车辆的位置。这些发现表明,反向投影摄影测量提供了一个可靠的手段,估计车辆的速度,当记录器定时适当地考虑和理解。通过量化不同记录系统和照明条件下的不确定性来源,本研究加强了在法医分析中使用视频导出的速度估计的证据基础,并支持其在案件工作中的辩护应用。
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引用次数: 0
Manhunt: Using Keatley’s Winthropping to help find fugitives 追捕:使用Keatley的Winthropping来帮助寻找逃犯
IF 2 4区 医学 Q2 MEDICINE, LEGAL Pub Date : 2025-11-19 DOI: 10.1016/j.scijus.2025.101357
D.A. Keatley
Tracking fugitives is an essential part of criminal investigations. When a fugitive is actively evading arrest, moving and hiding to escape, several issues arise. Technological advancements have greatly helped tracking individuals, yet still suspects are able to avoid arrest, sometimes for long periods of time. The current case study provides a review of an infamous fugitive case with modern decision-making analyses. The aim is to show how investigators can return to traditional investigative methods in situations wherein technology is lacking or ineffective. The outcome of the case study review shows the potential to track a fugitive’s behavior. It is suggested that this approach can be trialed and tested across other cold or historic cases to further improve the analyses. These desktop scenario sessions can provide effective training for real-time, current fugitive cases.
追踪逃犯是刑事侦查的重要组成部分。当逃犯积极逃避逮捕,移动和躲藏逃跑时,会出现几个问题。技术的进步极大地帮助了追踪个人,但嫌疑犯仍然能够避免被捕,有时是很长一段时间。当前的案例研究提供了一个臭名昭著的逃亡案件与现代决策分析的回顾。其目的是展示调查人员如何在缺乏技术或技术无效的情况下回归传统的调查方法。案例研究审查的结果显示了追踪逃犯行为的潜力。建议可以在其他冷案例或历史案例中对该方法进行试验和测试,以进一步改进分析。这些桌面场景课程可以为实时的、当前的逃犯案件提供有效的培训。
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引用次数: 0
Exploration of the multidisciplinary integration teaching model in forensic science: A case study of death by high fall after drinking alcohol 法医学多学科一体化教学模式探索——以酒后摔高致死为例
IF 2 4区 医学 Q2 MEDICINE, LEGAL Pub Date : 2025-11-13 DOI: 10.1016/j.scijus.2025.101356
An-Ding Zhou , Nian Zhou , Si-Hao Du , Xiao-Hui Tan , Xia Yue , Dong-Ri LI , Dong-Fang Qiao , Tong Xie , Qi Wang
Undergraduate education in forensic science plays a critical role in cultivating highly qualified forensic professionals with comprehensive analytical skills and practical competency. However, traditional teaching methods often prove insufficient when complex cases involving the intersection of multiple disciplines are encountered, and they frequently fail to meet the demands of practical teaching. This teaching practice is mainly for 4th-year undergraduate forensic science students and aims to explore a multidisciplinary integrated teaching model in forensic science on the basis of a complex simulated case: an alcohol-related fatality resulting from a fall from height. This model emphasizes student-centered learning, organically integrating forensic scene investigation, forensic pathological examination, and forensic toxicological analysis through the simulation of realistic case scenarios. It facilitates students in applying theoretical knowledge, mastering professional skills, and fostering evidence awareness, logical reasoning, teamwork abilities, standardized operational awareness, and a rigorous scientific attitude through practical experience. Through the teaching practice of simulating an alcohol-related fall from height fatality, the effectiveness of this model in enhancing students’ comprehensive analytical skills, practical competence, and forensic thinking provides a new perspective and approach for reforming undergraduate practical teaching in forensic science and lays a foundation for cultivating forensic professionals who are better equipped to meet societal needs in the future.
法医学本科教育对培养具有综合分析能力和实践能力的高素质司法专业人才具有重要作用。然而,当遇到涉及多学科交叉的复杂案例时,传统的教学方法往往显得不足,往往不能满足实践教学的需要。本教学实践主要针对法医学本科四年级学生,以一起醉酒坠楼致死的复杂模拟案例为基础,探索法医学多学科综合教学模式。该模式强调以学生为中心的学习,通过模拟真实案例场景,将法医现场勘查、法医病理检验、法医毒理学分析有机地结合起来。通过实践体验,培养学生运用理论知识,掌握专业技能,培养证据意识、逻辑推理能力、团队合作能力、规范操作意识和严谨的科学态度。通过模拟酒精坠楼致死的教学实践,该模式对学生综合分析能力、实践能力和法医思维的培养效果,为改革法医学本科实践教学提供了新的视角和途径,为培养未来更适应社会需要的法医专业人才奠定了基础。
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引用次数: 0
Beyond biohazards: teaching forensic biology practicals with synthetic and non-human samples 超越生物危害:用合成和非人类样本教授法医生物学实践
IF 2 4区 医学 Q2 MEDICINE, LEGAL Pub Date : 2025-11-07 DOI: 10.1016/j.scijus.2025.101354
Carrie Mullen , Jo-Ann Smith , Chloe Ward , Helen Page , Kayleigh Lamont , Sean Mallon , Farah Jaber-Hijazi
Practical laboratory classes are essential in forensic biology education, providing valuable hands-on experience that bridges theoretical knowledge and real-world practices. However, delivering authentic, safe, and legally compliant practical experiences presents significant challenges, particularly for programmes lacking access to specialised biohazard laboratories. The authors outline the development and implementation of synthetic alternatives to human body fluids, specifically saliva and semen, to minimise biohazard risks, alongside the in-house production of amylase detection paper (adapted from Phadebas® tablets), as a cost-effective alternative to expensive commercial options, helping resource-limited departments provide high-quality laboratory experiences for forensic and crime scene science students.
The work highlights the collaborative efforts of teaching and technical staff across institutions in developing these solutions, to support students in realistic forensic casework simulations while maintaining safety, legal compliance, and inclusivity. These innovations demonstrate how practical challenges in resource-limited departments can be overcome through sustainability-conscious design and pedagogical creativity, contributing to the wider goals of Education for Sustainable Development.
实践实验课程在法医生物学教育中是必不可少的,提供宝贵的实践经验,将理论知识与现实世界的实践联系起来。然而,提供真实、安全和合法的实践经验带来了重大挑战,特别是对于无法获得专业生物危害实验室的项目。作者概述了人类体液,特别是唾液和精液的合成替代品的开发和实施,以最大限度地减少生物危害风险,同时内部生产淀粉酶检测纸(改编自Phadebas®片剂),作为昂贵的商业选择的成本效益替代品,帮助资源有限的部门为法医和犯罪现场科学专业的学生提供高质量的实验室体验。这项工作强调了各机构的教学和技术人员在开发这些解决方案方面的合作努力,以支持学生进行现实的法医案例模拟,同时保持安全、法律合规和包容性。这些创新表明,资源有限的部门可以通过具有可持续意识的设计和教学创新来克服实际挑战,从而有助于实现可持续发展教育的更广泛目标。
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引用次数: 0
What is the best way to present likelihood ratios? A review of past research and recommendations for future research 呈现似然比的最佳方式是什么?回顾过去的研究和对未来研究的建议
IF 2 4区 医学 Q2 MEDICINE, LEGAL Pub Date : 2025-11-01 DOI: 10.1016/j.scijus.2025.101342
Geoffrey Stewart Morrison , Agnes S. Bali , Kristy A. Martire , Rebecca Hofstein Grady , William C. Thompson
As a first step in addressing the research question “What is the best way for forensic practitioners to present likelihood ratios so as to maximize their understandability for legal-decision makers?”, this paper reviews existing empirical literature on the comprehension of likelihood ratios by laypersons. The existing literature tends to research understanding of expressions of strength of evidence in general, rather than focusing specifically on likelihood ratios. We review the literature with respect to the CASOC indicators of comprehension (particularly sensitivity, orthodoxy, and coherence), and compare different formats that have been used to express likelihood ratios: numerical likelihood-ratios values, numerical random-match probabilities, and verbal strength-of-support statements (none of the studies that we reviewed tested comprehension of verbal likelihood ratios). We also critically review the studies with respect to methodology, and consider additional factors that could potentially assist with communication of the meaning of likelihood ratios. We conclude that the existing literature does not answer our research question, but, based on our review, we provide recommendations for the methodology of future research aimed at addressing our research question.
作为解决研究问题的第一步,“法医从业者提出可能性比的最佳方式是什么,以最大限度地提高法律决策者的可理解性?”,本文回顾了现有的外行人对似然比理解的实证文献。现有文献倾向于研究对一般证据强度表达的理解,而不是专门关注似然比。我们回顾了有关CASOC理解指标(特别是敏感性、正统性和连贯性)的文献,并比较了用于表达似然比的不同格式:数值似然比值、数值随机匹配概率和口头支持强度陈述(我们回顾的研究中没有一项测试了对言语似然比的理解)。我们还对研究方法进行了批判性的回顾,并考虑了可能有助于沟通似然比含义的其他因素。我们的结论是,现有的文献并不能回答我们的研究问题,但是,基于我们的回顾,我们为未来的研究方法提供建议,旨在解决我们的研究问题。
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引用次数: 0
Comparative analysis of fingermark development on biodegradable and traditional plastic garbage bags 可生物降解与传统塑料垃圾袋手印发育的对比分析
IF 2 4区 医学 Q2 MEDICINE, LEGAL Pub Date : 2025-11-01 DOI: 10.1016/j.scijus.2025.101350
Nienke Guijt , Annemieke van Dam
The escalating plastic waste crisis has increased the need for sustainable alternatives. Biodegradable plastics have emerged as a promising solution and are now frequently encountered at crime scenes. Although many studies have been published on the development of fingermarks on conventional plastics, limited research has been conducted on them on bioplastics. Therefore, this study aimed to evaluate the efficiency of various fingermark development techniques on biodegradable (d2w) plastic garbage bags compared to traditional (Dumil) plastic garbage bags. Fingermarks were deposited on one of the two types of plastics and developed with one of the following methods: magnetic powder (MP), wet powder (WP), cyanoacrylate fuming (CAF) with Basic Yellow 40, or Vacuum Metal Deposition (VMD). After deposition, fingermarks were left to age for eight different time periods upon development, ranging from fresh (24 h) up to 50 days (about 1 and a half months). Results demonstrated that high-quality fingermarks could be obtained using wet powder and cyanoacrylate fuming, specifically on biodegradable plastics. The effectiveness of magnetic powder on both types of plastic declined over time, while vacuum metal deposition showed inconsistent results. Overall, in this study, fingermarks on biodegradable plastics exhibited more ridge detail than those on traditional plastics. The inability to achieve uniform zinc evaporation during vacuum metal deposition development may explain the differences in fingermark development between the plastic types. This research underscores the need for forensic methodologies for biodegradable plastics to ensure reliable evidence collection in criminal investigations. Further studies are essential to enhance fingermark development techniques for these emerging materials.
不断升级的塑料垃圾危机增加了对可持续替代品的需求。可生物降解塑料已经成为一种很有前途的解决方案,现在经常在犯罪现场遇到。尽管已经发表了许多关于传统塑料上手印发展的研究,但对生物塑料上手印的研究却很少。因此,本研究旨在评价各种手印显现技术在生物降解(d2w)塑料垃圾袋上与传统(Dumil)塑料垃圾袋上的效果。将手印沉积在两种塑料中的一种上,并采用以下方法之一进行显影:磁粉(MP)、湿粉(WP)、碱性黄40氰基丙烯酸酯发烟(CAF)或真空金属沉积(VMD)。沉积后,留下手印,使其在发育过程中经过8个不同的时期,从新鲜(24小时)到50天(约1个半月)不等。结果表明,湿粉末和氰基丙烯酸酯熏烟可以获得高质量的手印,特别是在可生物降解塑料上。磁粉在两种塑料上的效果随着时间的推移而下降,而真空金属沉积的结果却不一致。总的来说,在这项研究中,可生物降解塑料上的手印比传统塑料上的手印显示出更多的脊状细节。在真空金属沉积显影过程中不能实现均匀的锌蒸发,这可能解释了塑料类型之间手印显影的差异。这项研究强调了对生物降解塑料的法医方法的需求,以确保在刑事调查中可靠的证据收集。进一步的研究是必要的,以提高这些新兴材料的手印开发技术。
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引用次数: 0
Breaking barriers: The admissibility of forensic evidence in US courts and the struggles with cutting-edge science 突破障碍:美国法庭上法医证据的可采性以及与前沿科学的斗争
IF 2 4区 医学 Q2 MEDICINE, LEGAL Pub Date : 2025-11-01 DOI: 10.1016/j.scijus.2025.101355
Ali Md. Ayub , Ferdous Robayet Syed , Haiqing Peng
This article critically examines two key scientific reports—Strengthening Forensic Science in the United States: A Path Forward and Forensic Science in Criminal Courts: Ensuring Scientific Validity of Feature-Comparison Methods—with a particular focus on their implications for the judiciary’s gatekeeping role in determining the admissibility of forensic evidence. It explores how U.S. courts address the validity and reliability of forensic methods in light of these landmark reports. Through an analysis of contemporary scholarship, judicial decisions, and legal standards, the study identifies significant obstacles to integrating the reports’ insights. These include inconsistencies in judicial practice, resistance from stakeholders, gaps in evidentiary standards, adversarial legal constraints, and a lack of scientific literacy among judges and attorneys. The authors argue that, as the ultimate arbiters of forensic evidence, courts must adopt strategies to incorporate scientific advancements, ensuring that unreliable forensic methods are excluded from judicial proceedings. This would incentivize the scientific community to standardize forensic methodologies across disciplines. Addressing these challenges requires legal reforms, the development of best practice guidelines, and enhanced judicial and legal education through interdisciplinary collaboration.
本文批判性地考察了两份重要的科学报告——《加强美国法医学:前进的道路》和《刑事法院的法医学:确保特征比较方法的科学有效性》,并特别关注了它们对司法部门在确定法医学证据的可采性方面的把关作用的影响。它探讨了美国法院如何在这些具有里程碑意义的报告中解决法医方法的有效性和可靠性。通过对当代学术、司法判决和法律标准的分析,该研究确定了整合报告见解的重大障碍。这些问题包括司法实践的不一致、利益相关者的抵制、证据标准的差距、对抗性的法律限制以及法官和律师缺乏科学素养。作者认为,作为法医证据的最终仲裁者,法院必须采取策略,结合科学进步,确保不可靠的法医方法被排除在司法程序之外。这将激励科学界跨学科标准化法医方法。应对这些挑战需要进行法律改革,制定最佳实践准则,并通过跨学科合作加强司法和法律教育。
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引用次数: 0
Correspondence by the Association of Forensic Science Providers (AFSP), Body Fluid Forum (BFF) on an article written by N. Dawnay and K. Sheppard: “From crime scene to courtroom: A review of the current bioanalytical evidence workflows used in rape and sexual assault investigations in the United Kingdom, Science & Justice 63 (2023) 206–228” 法医科学提供者协会(AFSP)、体液论坛(BFF)对N. Dawnay和K. Sheppard撰写的一篇文章的通信:“从犯罪现场到法庭:对英国强奸和性侵犯调查中使用的当前生物分析证据工作流程的回顾,科学与司法63(2023)206-228”
IF 2 4区 医学 Q2 MEDICINE, LEGAL Pub Date : 2025-11-01 DOI: 10.1016/j.scijus.2025.101317
Linda Groombridge, Geraldine Davidson, Sharon Doole, Jonathan Finnis, Isla Fraser, Charlotte Hargreaves, Rachel Knottley, Charlotte Murphy, Carol Rogers, Nighean Stevenson
“From crime scene to courtroom: A review of the current bioanalytical evidence workflows used in rape and sexual assault investigations in the United Kingdom” by N. Dawnay and K. Sheppard (Science & Justice 63 (2023) 206–228. https://doi.org/10.1016/j.scijus.2022.12.006) has been reviewed by the Body Fluid Forum (BFF) of the Association of Forensic Science Providers (AFSP). The BFF consists of a representative of each of the Forensic Science Providers within the UK and Ireland and as such, strives to provide best practice guidance for the examination and evaluation of biological evidence within cases involving rape and sexual assault. With this in mind, this article aims to address inaccuracies regarding laboratory (and scene) forensic examinations for body fluids and interpretation of the findings in respect of prosecution and defence propositions.
“从犯罪现场到法庭:对英国强奸和性侵犯调查中使用的当前生物分析证据工作流程的回顾”,作者N. Dawnay和K. Sheppard (Science & Justice 63(2023)) 206-228。https://doi.org/10.1016/j.scijus.2022.12.006)已由法医科学提供者协会(AFSP)的体液论坛(BFF)审查。BFF由英国和爱尔兰境内每个法医科学提供者的一名代表组成,因此,努力为涉及强奸和性侵犯案件的生物证据的检查和评估提供最佳实践指导。考虑到这一点,本文旨在解决关于实验室(和现场)体液法医检查的不准确之处以及对起诉和辩护主张的调查结果的解释。
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引用次数: 0
Re-evaluating forensic taphonomy experimental design: Relevance of non-human animal models vs. human donor bodies 重新评估法医埋藏学实验设计:非人类动物模型与人类供体的相关性
IF 2 4区 医学 Q2 MEDICINE, LEGAL Pub Date : 2025-11-01 DOI: 10.1016/j.scijus.2025.101348
Devin A. Finaughty , Victoria E. Gibbon , Craig A. Keyes , Desiré Brits
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引用次数: 0
Development and validation of a fast TEIS-TQMS screening method for the identification of 8 NPS in non-biological samples 非生物样品中8种NPS的快速TEIS-TQMS筛选方法的建立与验证
IF 2 4区 医学 Q2 MEDICINE, LEGAL Pub Date : 2025-11-01 DOI: 10.1016/j.scijus.2025.101349
Bartłomiej Feigel , Piotr Adamowicz , Sławomir Wybraniec
The number of New Psychoactive Substances (NPS) on the drug market is steadily increasing and there are no signs that this trend will change in the coming years. The constantly evolving drug market necessitates forensic experts to develop new methods for NPS analysis. With the growing number of analytes, the diversity of these compounds also increases, making it more challenging to develop screening methods that enable the analyses of a large number of the drugs simultaneously. It should also be noted that methods enabling quick NPS analysis are important due to constant changes in the popularity of “legal highs”, usually from several to a dozen of NPS are popular on the drug market at a given time. Therefore, it was decided to develop a research method for rapid screening determination of compounds from three groups of NPS: cannabinoids, tryptamines, and cathinones. The analyzed substances included 5-MEO-MIPT, N-ethylcathinone, UR-144, MDPBP, 3-MMC, XLR-11, 5F-AKB48, and alpha-PVP. As a result of the conducted work, a method enabling the analysis of samples containing 8 NPS in one minute, was developed. To achieve this, a Thermal Extraction Ion Source technique was applied in conjunction with a Triple Quadrupole Tandem Mass Spectrometry (TEIS-TQMS). TEIS can also be coupled with high-resolution mass spectrometers and ion trap mass analyzers for the analyte identification. The first use of TEIS-TQMS in the field of “legal highs” analysis is reported and its application enables the examination of “designer drugs” with a significant simplification of the sample preparation stage. Sample preparation for real samples involved a simple solid-liquid extraction with methanol and an addition of internal standard. Importantly, the high speed and efficiency of the TEIS-TQMS technique with analysis times as short as one minute per sample have the potential to substantially reduce the time required for toxicological evaluations, expert reporting, and overall case handling. This aspect is particularly valuable in forensic and prosecutorial workflows, where timely and reliable analytical results are crucial for supporting law enforcement investigations and informing prosecutorial decisions. As such, the proposed methodology may play a key role in streamlining the early stages of criminal proceedings, especially in cases involving large volumes of seized materials. The research method was validated in accordance with the ANSI/ASB norm. We have determined potential interferences, selectivity, matrix effects, and the limit of detection. Validation results included calculated limits of detection ranging from 0.01 to 6.47 µg/ml, matrix effects from −97% to 241%, and coefficients of variation from 9 to 84%. The usefulness of the developed method was confirmed by analyzing real samples and the results were subsequently validated using LC-MS/MS.
药品市场上的新型精神活性物质(NPS)的数量正在稳步增加,并且没有迹象表明这一趋势将在未来几年发生变化。不断发展的毒品市场要求法医专家开发新的NPS分析方法。随着分析物数量的增加,这些化合物的多样性也增加了,这使得开发能够同时分析大量药物的筛选方法更具挑战性。还应指出,能够快速分析NPS的方法很重要,因为“合法高点”的受欢迎程度不断变化,通常在给定时间内,药物市场上流行的NPS从几种到十几种。因此,决定开发一种快速筛选测定大麻素、色胺和卡西酮三组NPS化合物的研究方法。分析物质包括5-MEO-MIPT、n -乙基卡西酮、UR-144、MDPBP、3-MMC、XLR-11、5F-AKB48和α - pvp。根据所进行的工作,开发了一种在一分钟内分析含有8个NPS的样品的方法。为了实现这一目标,将热萃取离子源技术与三重四极杆串联质谱法(TEIS-TQMS)结合使用。TEIS还可以与高分辨率质谱仪和离子阱质量分析仪相结合,用于分析物鉴定。报告了TEIS-TQMS在“法定高点”分析领域的首次使用,其应用使“设计药”的检查具有显着简化的样品制备阶段。实际样品的样品制备包括用甲醇进行简单的固液萃取和加入内标。重要的是,TEIS-TQMS技术的高速度和效率,每个样品的分析时间短至1分钟,有可能大大减少毒理学评估、专家报告和整体病例处理所需的时间。这方面在法医和检察工作流程中特别有价值,因为及时和可靠的分析结果对于支持执法调查和为检察决定提供信息至关重要。因此,拟议的方法可能在精简刑事诉讼的早期阶段发挥关键作用,特别是在涉及大量扣押材料的案件中。根据ANSI/ASB规范对研究方法进行了验证。我们已经确定了潜在的干扰、选择性、基质效应和检测极限。验证结果包括计算检出限范围为0.01 ~ 6.47µg/ml,基质效应范围为- 97% ~ 241%,变异系数范围为9 ~ 84%。通过实际样品分析证实了该方法的有效性,随后用LC-MS/MS对结果进行了验证。
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