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Evaluation of phytosanitary products for the management of raspberry late leaf rust [Pucciniastrum americanum (Farl.) Arthur] 覆盆子晚叶锈病植物检疫产品评价[j]。亚瑟]
4区 农林科学 Q4 PLANT SCIENCES Pub Date : 2020-01-01 DOI: 10.7202/1072868ar
M. Delisle‐Houde, F. Demers, R. Tweddell
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引用次数: 1
Humic acid may retard damages of cells in strawberry apices in high saline environment 腐植酸对高盐环境下草莓尖部细胞的损伤有延缓作用
4区 农林科学 Q4 PLANT SCIENCES Pub Date : 2020-01-01 DOI: 10.7202/1072869ar
Yong-Hong Lin, Yao-Tsung Chang
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引用次数: 0
Évaluation de l’activité antibactérienne contre Xanthomonas campestris pv. vitians et Pseudomonas cichorii de différents extraits végétaux à base d’espèces horticoles et d’essences forestières 对油菜黄单胞菌的抑菌活性评价。以园艺和森林树种为基础的各种植物提取物中的葡萄和假单胞菌
4区 农林科学 Q4 PLANT SCIENCES Pub Date : 2019-01-01 DOI: 10.7202/1062449AR
Maxime Delisle-Houde, V. Toussaint, Russell J. Tweddell
Differents extraits hydro-ethanoliques a base de residus d’especes horticoles et d’essences forestieres ont ete testes in vitro pour leur activite antibacterienne envers Xanthomonas campestris pv. vitians et Pseudomonas cichorii, respectivement responsables de la tache bacterienne et de la maladie des taches et des nervures noires de la laitue. Des 17 extraits d’especes horticoles, 13 extraits affichent une concentration minimale letale (CML) ≥ 50 mg ml-1 envers X. campestris pv. vitians et P. cichorii. En contrepartie, trois des 20 extraits a base d’especes forestieres affichent une CML ≥ 50 mg ml-1 envers les deux bacteries. De faibles CMLs (≤ 6,25 mg ml-1) envers les deux bacteries ont ete obtenues avec l’extrait #3 a base de fruits de canneberge et les extraits de fruits d’aulne rugueux, d’ecorce de chene rouge, d’ecorce d’erable rouge et de rameaux de sapin baumier. Les resultats obtenus montrent que l’activite antibacterienne varie selon l’espece et la structure utilisees dans la preparation de l’extrait; les extraits d’essences forestieres presentant une plus forte activite antibacterienne. L’activite antibacterienne peut egalement varier selon le lot considere d’un meme extrait. Les extraits presentant une forte activite antibacterienne (CML ≤ 6,25 mg ml-1) seront testes au cours de travaux futurs afin d’evaluer leur efficacite a limiter le developpement des populations de X. campestris pv. vitians et P. cichorii sur les plants de laitue et a reprimer le developpement de la tache bacterienne et de la maladie des taches et des nervures noires.
以园艺和森林树种残渣为基础的不同水乙醇提取物对油菜黄单胞菌的体外抗菌活性进行了试验。葡萄和cichorii假单胞菌,分别负责生菜的细菌斑和黑斑和黑肋病。在17种蔬菜提取物中,13种提取物的最低致死浓度(CML)≥50 mg ml-1。葡萄和P. cichorii。另一方面,20种森林树种提取物中有3种对两种细菌的CML≥50 mg ml-1。以蔓越莓果实为基础的提取物#3和粗桤木果实、红橡树树皮、红枫树树皮和香脂冷杉树枝的提取物对这两种细菌的CMLs均较低(≤6.25 mg ml-1)。结果表明,抗菌活性因提取物制备的品种和结构而异;森林树种提取物具有较强的抗菌活性。抗菌活性也可能因同一提取物的不同批次而不同。具有较强抗菌活性(CML≤6.25 mg ml-1)的提取物将在未来的工作中进行测试,以评估其限制campestris pv种群发展的有效性。对莴苣植株上的葡萄和紫锥菊有抑制细菌斑和黑斑和黑肋病的发展的作用。
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引用次数: 0
Pathogenicity of Pratylenchus penetrans to dwarfing apple rootstocks 扁叶螨对苹果矮化砧木的渗透致病性
4区 农林科学 Q4 PLANT SCIENCES Pub Date : 2019-01-01 DOI: 10.7202/1059304AR
G. Bélair, N. Dauphinais, Y. Fournier
The objective of this research was to measure the effect of P. penetrans densities on the vegetative growth of three apple dwarfing rootstocks Bud 9, M.9 and M.26 under field microplot conditions. Under 500 nematodes kg-1 soil density, the shoot dry weights of Bud 9, M.9 and M.26 rootstocks were significantly reduced by 30%, 23% and 14% respectively. Under the 1 500 nematodes kg-1 soil density, the dry shoot weight of Bud 9 and M.9 were not significantly different from the 500 nematodes kg-1 soil density except M.26 which exhibited a 29% decrease. The root dry weights of rootstocks were not significantly reduced when exposed to these P. penetrans densities except M.26 which was reduced 26% relative to non-inoculated trees under the 1 500 nematodes kg-1 soil density. These results confirm the pathogenic effect of P. penetrans on dwarfing apple rootstocks. Although preliminary, these results suggest some tolerance of M.26 to P. penetrans under lower density when compared to M.9 and Bud 9. Under higher nematode densities, both Bud 9 and M.9 exhibited a tolerance of their root system in comparison to M.26. Our results also emphasize the need to further assess the susceptibility/tolerance of dwarfing apple rootstocks to various densities of P. penetrans.
在田间小田条件下,测定了透皮霉密度对3个苹果矮化砧木芽儿9号、芽儿9号和芽儿26营养生长的影响。在500线虫kg-1土壤密度下,芽9号、M.9号和M.26号砧木茎部干重分别显著降低30%、23%和14%。在1 500线虫kg-1土壤密度下,芽9和M.9的干茎重与500线虫kg-1土壤密度下差异不显著,但M.26的干茎重比500线虫kg-1土壤密度降低了29%。除M.26在1 500线虫kg-1土壤密度下比未接种的树木根系干重降低26%外,其余3种密度下的砧木根系干重均未显著降低。这些结果证实了穿透性假单胞菌对苹果砧木矮化的致病作用。虽然这些结果是初步的,但与M.9和芽9相比,这些结果表明M.26在较低密度下对穿透性假单胞菌有一定的耐受性。在较高的线虫密度下,芽9和芽9的根系对线虫的耐受性均优于芽26。我们的研究结果还强调了进一步评估矮化苹果砧木对不同密度渗透线虫的敏感性/耐受性的必要性。
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引用次数: 0
Ophiostoma ulmi DNA naturally introgressed into an isolate of Ophiostoma novo-ulmi is clustered around pathogenicity and mating type loci 自然渗入到新乌尔米蛇口的DNA聚集在致病性和交配型位点周围
4区 农林科学 Q4 PLANT SCIENCES Pub Date : 2019-01-01 DOI: 10.7202/1059303AR
Abdelali Et-Touil, M. Dusabenyagasani, G. Bouvet, C. Brasier, L. Bernier
The invasive fungal pathogens Ophiostoma ulmi and O. novo-ulmi have caused two successive pandemics of Dutch elm disease since the beginning of the 20th century. In nature, the highly aggressive O. novo-ulmi may hybridize with the less aggressive O. ulmi. Growth rate and molecular analyses were conducted on an unusual, moderately aggressive O. novo-ulmi isolate, AST27, carrying an introgressed pathogenicity gene, Pat1-m; on highly aggressive O. novo-ulmi isolate H327; on O. ulmi isolates Q412T and W9; and on progeny from laboratory crosses between H327 and AST27. Genetic analysis indicated that the Pat1 and Mat1 (mating type) loci were in different linkage groups corresponding to O. novo-ulmi H327 chromosomes 1 and 2, respectively. Most of the molecular differences between the nuclear genomes of H327 and AST27 occurred in the vicinity of Pat1 and Mat1. In addition, two putative quanti-tative trait loci, Mgr1 and Mgr2, which influence mycelial growth rate at 21°C and 28°C, the optima for O. novo-ulmi and O. ulmi, were linked to Mat1 and Pat1, respectively.
自20世纪初以来,侵袭性真菌病原菌ulmi和O. novoo -ulmi引起了两次连续的荷兰榆树病大流行。在自然界中,高攻击性的O. novoo -ulmi可能与攻击性较弱的O. ulmi杂交。对一种罕见的中等侵袭性O. novoo -ulmi分离物AST27进行了生长速率和分子分析,该分离物携带渐渗致病性基因Pat1-m;对高侵袭性新芽孢杆菌分离株H327;菌株Q412T和W9的研究以及H327和AST27实验室杂交的后代。遗传分析表明,该位点分别位于O. novoo -ulmi H327染色体1号和2号对应的不同连锁群中。H327和AST27核基因组的分子差异大部分发生在Pat1和Mat1附近。此外,两个推测的数量性状位点Mgr1和Mgr2分别与Mat1和Pat1连锁,这两个位点在21°C和28°C条件下影响菌丝生长速率,这两个位点是O. novoo -ulmi和O. ulmi的最佳位点。
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引用次数: 7
Virus et vigne, un mariage difficile à défaire : la biovigilance est nécessaire plus que jamais 病毒和葡萄,一个难以打破的联姻:生物警戒比以往任何时候都更有必要
4区 农林科学 Q4 PLANT SCIENCES Pub Date : 2019-01-01 DOI: 10.7202/1059305AR
Issam E. Ben Moussa, Pierre Lemoyne, M. Fall
La viticulture est l’une des industries les plus largement etablies dans le monde. Au Canada, apres l’Ontario et la Colombie-Britannique, c’est au tour du Quebec de connaitre une intensification de la viticulture. Or, plusieurs contraintes sont et seront associees a cette intensification. La vigne est une plante vivace caracterisee par une croissance indefinie impliquant une disponibilite continue de tissus tendres et eventuellement une susceptibilite aux insectes et maladies. Depuis ses origines, la vigne a evolue en constante dualite avec les virus. Plus de 60 virus sont actuellement associes a la vigne a l’echelle mondiale, dont la majorite des virus sont pathogenes, pouvant reduire la vigueur et la productivite des vignes ou la qualite des raisins. Au Quebec, la derniere enquete qui fait etat des virus dans les vignobles date des annees 1990. Cette enquete a revele la presence de deux virus pathogenes de la vigne alors que l’ensemble des virus presents dans les vignobles du Quebec restent toujours a definir. Ce manquement de connaissance est sans doute l’un des facteurs qui expliquent l’inefficacite des strategies de lutte contre les viroses de la vigne. Ces strategies reposent essentiellement sur la prevention et sur des programmes de certification du materiel viticole en ciblant des virus connus et detectables. Dans cet article, nous evoquons les lacunes associees a l’etat des virus de la vigne au Quebec et nous expliquons comment un programme de biovigilance peut aider a mitiger les menaces et assurer une lutte efficace contre les viroses de la vigne.
葡萄栽培是世界上建立最广泛的产业之一。在加拿大,继安大略省和不列颠哥伦比亚省之后,现在轮到魁北克加强葡萄种植了。现在和将来都有一些限制因素与这种加强有关。葡萄藤是一种多年生植物,其特点是不确定的生长,这意味着柔软的组织持续可用,可能容易受到昆虫和疾病的影响。从一开始,葡萄树就一直在与病毒的二元性中进化。目前全世界有60多种与葡萄有关的病毒,其中大多数是致病性病毒,可以降低葡萄的活力和生产力或葡萄的质量。在魁北克,上一次关于葡萄园病毒的调查可以追溯到20世纪90年代。这次调查揭示了两种葡萄致病性病毒的存在,而魁北克葡萄园中存在的所有病毒仍有待确定。这种知识的缺乏无疑是控制葡萄病毒策略无效的原因之一。这些战略主要基于预防和认证计划,针对已知和可检测的病毒。在这篇文章中,我们讨论了与魁北克葡萄病毒状况相关的差距,并解释了生物警戒计划如何帮助减轻威胁并确保有效控制葡萄病毒。
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引用次数: 2
Effects of exogenous application of salicylic acid on drought performance of medicinal plant, Fritillaria przewalskii Maxim 外源施用水杨酸对药用植物贝母抗旱性能的影响
4区 农林科学 Q4 PLANT SCIENCES Pub Date : 2019-01-01 DOI: 10.7202/1066455ar
Ruili Ma, Shengrong Xu, Yuan Chen, F. Guo, rui Wu, S. Okyere, Fusheng Wang, Y. Jing, Wang Xingzheng
This study investigated the effects of salicylic acid (SA) foliar application on Fritillariaprzewalskii under drought stress condition. Plants were subjected to three irrigation regimes, 75-80% control (CK), 60-65% medium stress (M) and 40-45% severe stress (S) of the field capacity and three levels of SA, 0.0, 0.5 and 1.0 mM. Relative water content (RWC), proline content, total soluble carbohydrates, chlorophyll “a” (Chl a), chlorophyll “b” (Chl b), chlorophyll “a + b” (Chl a + b), carotenoids contents, malondialdehyde (MDA) contents and activities of several antioxidant enzymes such as superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), glutathione reductase (GR) and ascorbate peroxidase (APX) activity were measured. RWC, soluble carbohydrates, Chl b, Chl a + b, MDA content and activities of antioxidant enzymes (SOD, POD, CAT, GR and APX) were significantly affected by water deprivation without SA. Exogenous SA significantly increased the content of RWC, total leaf soluble carbohydrates, leaf proline and Chl b at moderate water deficit and severe water deficit. MDA content was decreased significantly by exogenous SA. The activities of antioxidant enzymes (SOD, POD, CAT, GR and APX) were also significantly affected by exogenous SA. However, the content of Chl a, Chl a + b, and carotenoids were not significantly affected by exogenous SA.
研究了干旱胁迫条件下水杨酸(SA)叶面施用对贝母生长的影响。对照75-80%对照(CK)、60-65%中度胁迫(M)和40-45%重度胁迫(S) 3种灌溉方式,田间容量分别为0.0、0.5和1.0 mM。相对含水量(RWC)、脯氨酸含量、总可溶性碳水化合物、叶绿素“a”(Chl a)、叶绿素“b”(Chl b)、叶绿素“a + b”(Chl a + b)、类胡萝卜素含量、丙二醛(MDA)含量和超氧化物歧化酶(SOD)等几种抗氧化酶的活性、测定过氧化物酶(POD)、过氧化氢酶(CAT)、谷胱甘肽还原酶(GR)和抗坏血酸过氧化物酶(APX)活性。不加SA的缺水处理显著影响了RWC、可溶性碳水化合物、Chl b、Chl a + b、MDA含量和抗氧化酶(SOD、POD、CAT、GR和APX)活性。在中度亏水和重度亏水条件下,外源SA显著提高了叶片RWC、总可溶性碳水化合物、脯氨酸和Chl b含量。外源SA显著降低MDA含量。抗氧化酶(SOD、POD、CAT、GR和APX)的活性也受到外源SA的显著影响。外源SA对Chl a、Chl a + b和类胡萝卜素含量影响不显著。
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引用次数: 2
Survey of imazethapyr-resistant common ragweed (Ambrosia artemisiifolia L.) in Quebec 魁北克普通豚草(Ambrosia artemisiifolia L.)抗吡虫胺调查
4区 农林科学 Q4 PLANT SCIENCES Pub Date : 2019-01-01 DOI: 10.7202/1066456ar
Félix Marsan-Pelletier, A. Vanasse, Marie-Josée Simard, M. Cuerrier
Common ragweed (Ambrosia artemisiifolia L.) is widespread in soybean (Glycine max L.) fields in southern Quebec. Biotypes resistant to ALS (group 2) herbicides are commonly reported in conventional crops where these herbicides are used. Reported cases are voluntary and potentially underestimate the occurrence of resistance. A survey was therefore undertaken in 2014 and 2015 in soybean fields treated with a Group 2 herbicide. Common ragweed seeds were collected from 123 fields. Seedlings were grown and tested for resistance using the recommended rate of imazethapyr (100.8 g a.e. ha-1). Weed populations were classified as susceptible, developing resistance (less than one third of plants classified as resistant) or resistant (at least one third of plants were resistant). Twenty populations were then selected based on these resistance levels and treated with four doses of the herbicide (0, 100.8, 201.6, and 403.2 g a.e. ha-1). Resistance to imazethapyr was detected in 81% of samples (21.1% were classified as developing resistance and 59.4% were classified as resistant). Populations classified as developing resistance had a resistance factor of 1.04, while populations classified as resistant had a resistance factor greater than 5. These results confirm the presence of multiple populations of imazethapyr-resistant common ragweed in Quebec.
普通豚草(Ambrosia artemisiifolia L.)广泛存在于魁北克南部的大豆(Glycine max L.)田里。在使用ALS(2类)除草剂的常规作物中,通常报道了抗ALS(2类)除草剂的生物型。报告的病例是自愿的,可能低估了耐药性的发生。因此,2014年和2015年对使用2类除草剂的大豆田进行了调查。从123块地采集豚草种子。幼苗生长并使用推荐剂量(100.8 g a.e. ha-1)进行抗性测试。杂草种群被分类为易感,产生抗性(少于三分之一的植物被分类为抗性)或抗性(至少三分之一的植物具有抗性)。然后根据这些抗性水平选择20个种群,分别用4种剂量(0、100.8、201.6和403.2 g a.e. ha-1)处理。81%的标本检测到耐药,其中21.1%为正在形成耐药,59.4%为耐药。发生抗性种群的抗性因子为1.04,抗性种群的抗性因子大于5。这些结果证实了魁北克存在多个抗吡虫啉普通豚草种群。
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引用次数: 1
Management of Pratylenchus penetrans and Verticilllium symptoms in strawberry 草莓穿透型叶盘虫和黄斑病症状的防治
4区 农林科学 Q4 PLANT SCIENCES Pub Date : 2018-05-31 DOI: 10.7202/1046783AR
G. Bélair, J. Coulombe, N. Dauphinais
Under field conditions, the effect of a single rotation with corn, cruciferous crops (canola followed by white mustard) as green manure, oats, and forage pearl millet was measured on the density of Pratylenchus penetrans and its impact on damage and losses caused by Verticillium dahliae in a strawberry plantation the following year. The lowest density of P. penetrans was recorded following forage pearl millet and green cruciferous manure, and in both cases, it was below the known pest threshold in strawberry of 500 P. penetrans kg-1 soil. Both green manure of cruciferous plants and forage pearl millet reduced the incidence of Verticillium wilt and increased the growth of strawberry plants. In the fall, the number of crowns and the number of daughter plants were significantly higher following forage pearl millet or cruciferous plants than corn. The highest wilt symptoms and the lowest strawberry growth were observed in plots previously planted with corn, which also harboured the highest spring populations of P. penetrans. Those results support a positive interaction between P. penetrans and V. dahliae, even more importantly so on susceptible cultivard such as ‘Jewell’.
在大田条件下,以玉米、十字花科作物(油菜先白芥菜)为绿肥、燕麦和饲料珍珠粟,单次轮作,测定了次年草莓园透蚜密度及其对大大花黄萎病危害和损失的影响。在500穿山甲kg-1土壤中,草莓的穿山甲密度均低于已知阈值,最低的是饲料珍珠粟和绿色十字花科肥料。十字花科植物绿肥和饲料珍珠粟均能降低黄萎病的发病率,促进草莓植株的生长。在秋季,采食珍珠粟和十字花科植物后的冠数和子代数显著高于采食玉米。在以前种植过玉米的地块上,观察到枯萎病症状最严重,草莓生长最低,而且这些地块也有最高的春季渗透线虫种群。这些结果支持了穿透性假单胞菌与大丽花之间的正交互作用,甚至更重要的是在“朱厄尔”等易感品种上。
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引用次数: 4
Antagonistic potential of native agrocin-producing non-pathogenic Agrobacterium tumefaciens strain UHFBA-218 to control crown gall in peach 产农蛋白非致病性农杆菌UHFBA-218对桃树冠瘿病的拮抗作用
4区 农林科学 Q4 PLANT SCIENCES Pub Date : 2017-07-13 DOI: 10.7202/1040509AR
Aditi Sharma, A. Gupta, K. Khosla, R. Mahajan, P. K. Mahajan
A non-pathogenic agrocin-producing native isolate of Agrobacterium tumefaciens strain UHFBA-218 was tested as a biological control agent against the peach crown gall. This strain was compatible with all the recommended pesticides used in stone fruits in the integrated pest management (IPM) module, except for copper oxychloride, which was detrimental to its growth. Upon artificial co-inoculation of 4-wk-old plants of tomato var. Solan Gola with A. tumefaciens strain UHFBA-218 and tumorigenic A. tumefaciens strain Peach 2E-10, out of the 27 isolates recovered, six were transconjugants showing selective acquisition of tumorigenic factors as made evident by amplification with ipt and virD2 primers, whereas the rest of the isolates did not acquire any of these tumorigenic factors. A white stone powder-based formulation of this isolate (103.3 × 108 cfu g-1) retained appreciable viability for up to 6 months at room temperature. When peach roots and seeds were soaked in cell suspensions of different doses of a white stone powder-based bioformulation of UHFBA-218 before planting in the field, the number of plants with tumours was reduced, with the lowest incidence of crown gall being observed in the 0.1% UHFBA-218 root dip treatment, i.e. 1.48% and 0.80% during the years 2013 and 2014, respectively. No incidence of crown gall was observed in the three seed dip treatments, i.e. 30-min dip in UHFBA-218 followed by 1 h of shade drying, stratified seeds dipped for 30 min in 0.1% suspensions of strains UHFBA-218 or K84 followed by 1 h of shade drying before sowing, as compared with 14.76% incidence in untreated plants.
研究了一株产农蛋白的农杆菌UHFBA-218原生分离物作为防治桃冠瘿病的生物防治剂。该菌株与核果综合治理(IPM)模块中推荐使用的所有农药均兼容,但对其生长有害的氯氧化铜除外。将番茄品种Solan Gola的4周龄植株与产瘤假丝酵母菌UHFBA-218和致瘤假丝酵母菌Peach 2E-10人工共接种后,在27株分离株中,有6株通过ipt和virD2引物扩增得到了选择性获得致瘤因子的转接合物,而其余分离株没有获得任何致瘤因子。该分离物的白色石粉配方(103.3 × 108 cfu g-1)在室温下可保持可观的活力长达6个月。种植前将桃根和种子浸泡在不同剂量的UHFBA-218白色石粉生物制剂的细胞悬浮液中,可减少肿瘤植株的数量,其中0.1% UHFBA-218根浸处理的树冠瘤发生率最低,2013年和2014年分别为1.48%和0.80%。在UHFBA-218中浸种30 min后阴晒1 h,在UHFBA-218或K84的0.1%悬浮液中分层种子浸种30 min后阴晒1 h后播种,三种处理均未发现冠瘿发生,而未处理植株的冠瘿发生率为14.76%。
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引用次数: 5
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Phytoprotection
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