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Antimicrobial efficacy of cinnamon, ginger, horseradish and nutmeg extracts against spoilage pathogens 肉桂、生姜、辣根和肉豆蔻提取物对腐败病原体的抗菌作用
4区 农林科学 Q4 PLANT SCIENCES Pub Date : 2010-06-28 DOI: 10.7202/044024AR
Hortense N. Mvuemba, S. E. Green, A. Tsopmo, T. Avis
In the search for alternatives to the use of synthetic fungicides, aqueous spice extracts were evaluated for their effects on the mycelial growth of various spoilage pathogens and their ability to control potato dry rot and carrot cavity spot in vivo. Results showed that cinnamon, ginger and nutmeg significantly inhibited the mycelial growth of Aspergillus niger (Ascomycota), Fusarium sambucinum (Ascomycota), Pythium sulcatum (Oomycota) or Rhizopus stolonifer (Zygomycota), whereas horseradish extract did not lead to the inhibition of any microorganism at the tested concentration. Among the most effective extracts, 0.05 g mL‑1 of cinnamon extract completely inhibited A. niger and P. sulcatum, and 0.10 g mL‑1 of cinnamon extract completely inhibited F. sambucinum. A concentration of 0.05 g mL‑1 of ginger extract also caused 100% inhibition of P. sulcatum. In vivo, cinnamon extract significantly reduced lesions of potato dry rot and carrot cavity spot, and ginger extract reduced lesions of carrot cavity spot. These results indicate that aqueous cinnamon and ginger extracts could provide an alternative to the use of synthetic fungicides to control these pathogens.
为了寻找合成杀菌剂的替代品,研究了香料水提取物对各种腐败病菌菌丝生长的影响,以及在体内控制马铃薯干腐病和胡萝卜空洞病的能力。结果表明,肉桂、生姜和肉豆蔻均能显著抑制黑曲霉(子囊菌科)、sambucinum(子囊菌科)、sulcatum(卵菌科)和stolonifer根霉(颧菌科)的菌丝生长,而辣根提取物在试验浓度下对任何微生物均无抑制作用。在最有效的提取物中,0.05 g mL - 1的肉桂提取物完全抑制黑曲霉和紫霉,0.10 g mL - 1的肉桂提取物完全抑制桑布辛菌。浓度为0.05 g mL - 1的生姜提取物也能100%抑制短叶假单胞菌。在体内,肉桂提取物显著减少马铃薯干腐病和胡萝卜空洞斑的病变,姜提取物显著减少胡萝卜空洞斑的病变。这些结果表明,肉桂和生姜水提物可以替代人工合成的杀菌剂来控制这些病原体。
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引用次数: 21
Differentiation of entomopathogenic fungus Beauveria bassiana (Ascomycetes: Hypocreales) isolates by PCR-RFLP 昆虫病原真菌球孢白僵菌(子囊菌:下creales)分离物的PCR-RFLP分化
4区 农林科学 Q4 PLANT SCIENCES Pub Date : 2010-06-28 DOI: 10.7202/044022AR
Rachid Sabbahi, R. Lavallée, A. Merzouki, C. Guertin
The entomopathogenic fungus Beauveria bassiana is a promising biological control agent of several insect pests in agriculture. Molecular approaches (PCR, DNA sequence analysis and PCR-RFLP) were used in our research as tools for the identification of different B. bassiana isolates. Our work consisted in identifying the 18S, ITS1, 5.8S, ITS2 and 28S regions of B. bassiana ribosomal DNA. The DNA sequences of the amplified regions showed that the 18S rDNA is the most conserved unit, with a high homology (99.5%) between the isolates studied, while the 3’ end of the 28S rDNA has a great variability, which makes it possible to differentiate the isolates. The PCR-RFLP method was used to monitor isolates of B. bassiana and distinguish them in a target pest, Lygus lineolaris. This method involved two main steps. First, PCR was used to amplify a region of the 28S gene of B. bassiana. Second, this PCR product was digested using restriction endonucleases, and the fragments produced were compared using gel electrophoresis. Because of the high specificity and sensitivity of PCR-RFLP, it was possible to discriminate between B. bassiana isolates using spores scraped from the surface of an infected insect as samples.
球孢白僵菌(Beauveria bassiana)是一种很有前途的农业害虫生物防治剂。采用分子方法(PCR、DNA序列分析和PCR- rflp)对不同的球孢白僵菌进行鉴定。我们的工作包括鉴定球孢白孢核糖体DNA的18S、ITS1、5.8S、ITS2和28S区域。扩增区域的DNA序列表明,18S rDNA是最保守的单位,同源性高(99.5%),而28S rDNA 3′端具有很大的变异性,这为区分菌株提供了可能。采用PCR-RFLP方法对球孢白僵菌分离株进行了监测,并与目标害虫线线虫进行了区分。这种方法包括两个主要步骤。首先,利用PCR扩增球孢白僵菌28S基因的一个区域。其次,用限制性内切酶酶切该PCR产物,并用凝胶电泳对产生的片段进行比较。由于PCR-RFLP的高特异性和敏感性,从被感染昆虫的表面刮取孢子作为样本,可以区分球孢白僵菌分离株。
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引用次数: 9
A new stripe rust resistance gene transferred from Thinopyrum intermedium to hexaploid wheat (Triticum aestivum) 一种新的抗条锈病基因转介至六倍体小麦(Triticum aestivum)
4区 农林科学 Q4 PLANT SCIENCES Pub Date : 2010-06-28 DOI: 10.7202/044023AR
P. Luo, Xueyun Hu, Z. Chang, M. Zhang, H. Zhang, Z. Ren
Wheat stripe rust (Puccinia striiforis f. sp. tritici) races CYR31 and CYR32, prevalent in China, are virulent to many wheat stripe rust resistance genes (Yr genes). To expand the availability of effective resistance to CYR31 and CYR32, stripe rust resistance was transferred from intermediate wheatgrass (Thinopyrum intermedium) to common wheat (Triticum aestivum). The susceptible wheat cultivar CM107 was crossed with amphiploid TAI7047, derived from the wide cross Taiyuan768/Thinopyrum intermedium//76(64). Two wheat lines originating from the cross, YU24 and YU25, were resistant to CYR31 and CYR32. Pedigree analysis showed that the resistance to stripe rust in YU24 and YU25 originated from intermediate wheatgrass. Genetic analyses indicated that the resistance to stripe rust is controlled by a single dominant gene. Allelic tests determined that the resistance gene(s) in YU24 and YU25 are identical. The new gene has temporarily been designated as YrYU25. SSR and RAPD analyses showed that YrYU25 was introduced by cryptic translocation into common wheat.
小麦条锈病(Puccinia striiforis f. sp. tritici)在中国流行,CYR31和CYR32对许多小麦条锈病抗性基因(Yr基因)具有毒力。为了扩大对CYR31和CYR32有效抗性的有效性,将中间小麦(Thinopyrum intermedium)的条锈病抗性转移到普通小麦(Triticum aestivum)上。将敏感小麦品种CM107与宽杂交品种Taiyuan768/Thinopyrum intermedium//76衍生的两倍体TAI7047进行杂交。由该杂交组合产生的两个小麦品系YU24和YU25对CYR31和CYR32具有抗性。系谱分析表明,育24和育25抗条锈病的基因来源于中间麦草。遗传分析表明,小麦对条锈病的抗性是由一个显性基因控制的。等位基因试验表明,玉24和玉25的抗性基因完全相同。新基因暂时被命名为YrYU25。SSR和RAPD分析表明,YrYU25是通过隐性转位引入普通小麦的。
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引用次数: 16
Toxicity effects of an insecticidal soap on the green peach aphid [Homoptera: Aphididae] 一种杀虫皂对桃蚜的毒力研究[同翅目:蚜科]
4区 农林科学 Q4 PLANT SCIENCES Pub Date : 2010-01-27 DOI: 10.7202/038985AR
E. Tremblay, A. Bélanger, M. Brosseau, G. Boivin
Effects of an insecticidal soap on the survival, development and reproduction of the green peach aphid, Myzus persicae, were studied. Twenty-four hours after application at a concentration of 37.50 g L-1, the soap caused 100% mortality in all aphid instars, and LC50 (lethal concentrations causing 50% mortality in the population) were 1.50, 3.25 and 5.50 g L-1 for first and second instars, third and fourth instars, and adult M. persicae, respectively. Aphids that survived the LC50 had a shorter longevity than the controls. Both insecticidal soap and parasitoids could be used on a crop but, as the LC50 of the third and fourth instars and adult M. persicae are higher than that of the aphid parasitoid Aphidius colemani (2.75 g L-1), it is essential to avoid treating aphids with insecticidal soap when adult parasitoids are present in the crop.
研究了一种杀虫皂对桃蚜(Myzus persicae)存活、发育和繁殖的影响。施用后24 h,皂液浓度为37.50 g L-1,对1、2龄、3、4龄和成虫的LC50分别为1.50、3.25和5.50 g L-1,对所有蚜虫的死亡率均为100%。在LC50环境下存活下来的蚜虫寿命比对照组短。杀虫皂和拟寄生蜂均可用于作物,但由于桃蚜3、4龄和成虫的LC50高于拟寄生蜂蚜的LC50 (2.75 g L-1),因此当作物中存在成虫时,应避免使用杀虫皂处理蚜虫。
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引用次数: 4
Efficacy of fungicide mixtures for the management of Phytophthora infestans (US-1) on potato 混配杀菌剂对马铃薯马铃薯疫霉(US-1)的防治效果
4区 农林科学 Q4 PLANT SCIENCES Pub Date : 2010-01-27 DOI: 10.7202/038983AR
F. Muchiri, R. Narla, O. M. Olanya, R. Nyankanga, E. Ariga
Fungicide application strategies (timing, frequency, rates and mixtures) are important for the control and resistance management of potato late blight caused by Phytophthora infestans. The efficacy of fungicide mixtures consisting of fenamidone + mancozeb and propamocarb HCL + mancozeb at various rates and in spray regimes containing metalaxyl and mancozeb was evaluated for late blight control (US-1) at four locations in Kenya. Propamocarb HCL + mancozeb significantly (P < 0.05) reduced foliar blight compared with mancozeb and the untreated control under moderate to severe disease pressure. Disease severity was significantly lower following application of propamocarb HCL + mancozeb at a rate of 4L ha-1 than at rates of 2L and 3L ha-1 in 1999 and 2000, but it was not significantly lower following applications at a rate of 3L ha-1 in 2000 and 2001. There were no significant differences in mean final late blight score among the three rates of 0.9, 1.0 and 1.1 kg ha-1 of fenamidone + mancozeb. All fungicide mixtures and application sequences significantly reduced the area under the disease progress curve and final late blight scores as compared with the unprotected control. Total and marketable tuber yield significantly (P < 0.05) increased in all fungicide-treated plots.
在马铃薯晚疫病防治和抗性管理中,杀菌剂的施用策略(时间、频率、用量和混合)具有重要意义。在肯尼亚的四个地点,评估了由非胺酮+代森锰锌和丙胺威HCL +代森锰锌组成的杀菌剂混合物以不同剂量和含有甲螨灵和代森锰锌的喷雾方式防治晚疫病(US-1)的效果。在中重度疾病压力下,与代森锰锌和未处理对照相比,丙胺威+代森锰锌显著(P < 0.05)降低了叶枯病。1999年和2000年,施用4L ha-1的丙氨威+代森锌显著低于施用2L和3L ha-1的剂量,但在2000年和2001年,施用3L ha-1的剂量并没有显著降低疾病严重程度。非那米酮+代森锰锌在0.9、1.0和1.1 kg hm -1处理下的平均最终晚疫病评分差异不显著。与无保护对照相比,所有杀菌剂混合物和施用顺序均显著降低了病害进展曲线下的面积和最终晚疫病评分。杀菌剂处理后块茎总产量和可售块茎产量均显著提高(P < 0.05)。
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引用次数: 13
Fumonisin B1 and zearalenone contamination of wheat in Croatia and influence of fungicide treatments 克罗地亚小麦伏马菌素B1和玉米赤霉烯酮污染及杀菌剂处理的影响
4区 农林科学 Q4 PLANT SCIENCES Pub Date : 2010-01-27 DOI: 10.7202/038984AR
D. Ivić, A. Domijan, M. Peraica, B. Cvjetković
In Croatia, a trial was conducted to determine the presence of the Fusarium mycotoxins fumonisin B1 and zearalenone in wheat kernels and to evaluate the efficacy of nine fungicides on Fusarium head blight severity as well as fumonisin B1 and zearalenone accumulation in wheat grain. Fumonisin B1 and zearalenone were detected in all grain samples in mean concentrations ranging from 182.0 to 446.6 µg kg-1 (fumonisin B1) and from 2.59 to 5.33 µg kg-1 (zearalenone). No significant differences were found among fumonisin B1 and zearalenone content in wheat grain for the different fungicide treatments. No correlation was revealed between Fusarium head blight severity and fumonisin B1 or zearalenone content in wheat grain, nor between fungicide efficacy and fumonisin B1 or zearalenone content in wheat grain. Under conditions of high disease pressure, efficacy of the fungicides was between 85.7% (tebuconazole + triadimefon) and 72.1% (carbendazim).
在克罗地亚,进行了一项试验,以确定小麦籽粒中是否存在镰刀菌毒素伏马菌素B1和玉米赤霉烯酮,并评估9种杀菌剂对小麦赤霉病严重程度以及伏马菌素B1和玉米赤霉烯酮在小麦籽粒中的积累的效果。在所有谷物样品中检测到伏马菌素B1和玉米赤霉烯酮的平均浓度分别为182.0 ~ 446.6µg kg-1(伏马菌素B1)和2.59 ~ 5.33µg kg-1(玉米赤霉烯酮)。不同杀菌剂处理小麦籽粒中伏马菌素B1和玉米赤霉烯酮含量无显著差异。小麦赤霉病严重程度与伏马菌素B1或玉米赤霉烯酮含量无相关性,杀菌剂效果与伏马菌素B1或玉米赤霉烯酮含量无相关性。在高病压条件下,杀菌剂的杀菌率分别为85.7%(戊康唑+三唑美丰)和72.1%(多菌灵)。
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引用次数: 8
Seasonal variation in Blueberry scorch virus concentration in highbush blueberry and implications for disease monitoring and management 高丛蓝莓中蓝莓焦病毒浓度的季节变化及其对疾病监测和管理的意义
4区 农林科学 Q4 PLANT SCIENCES Pub Date : 2010-01-27 DOI: 10.7202/038982AR
D. Raworth, S. Mathur
Seasonal variation in relative Blueberry scorch virus (BlScV) concentration was determined for three infected highbush blueberry, Vaccinium corymbosum, cultivars in a commercial field in southwestern British Columbia, Canada. Relative virus concentration per g of infected blueberry flower clusters and leaf terminal tissue varied during the season with significant cultivar-by-time interactions. Flower clusters had the highest BlScV concentration per g of tissue and could be collected in early May for disease surveys. Timing of leaf sample collection for BlScV surveys, transmission studies and virus purification should be based on studies of temporal variation in BlScV concentration for the principal cultivars in a production area.
在加拿大不列颠哥伦比亚省西南部的一处商业田中,测定了三种高丛蓝莓(vacinium corymbosum)感染的蓝莓焦枯病毒(BlScV)相对浓度的季节变化。每g感染蓝莓花簇和叶顶组织的相对病毒浓度随季节变化而变化,具有显著的品种间相互作用。花簇中每g组织中BlScV浓度最高,可在5月初采集,用于病害调查。BlScV调查、传播研究和病毒纯化的叶片样本采集时间应基于生产区主要品种BlScV浓度的时间变化研究。
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引用次数: 0
La malherbologie au coeur des enjeux du XXIe siècle malherbology是21世纪挑战的核心
4区 农林科学 Q4 PLANT SCIENCES Pub Date : 2010-01-27 DOI: 10.7202/038981AR
Anne Légère
Ce texte a pour objectif de cerner la dimension malherbologique de nombreux enjeux contemporains, agricoles et autres, alors que paradoxalement les ressources scientifiques et techniques dans cette discipline se font de plus en plus rares. L’adoption recente des cultures transgeniques resistantes aux herbicides a permis de caracteriser les problemes agroecologiques associes aux flux geniques et a la persistance de transgenes dans l’environnement. Les questions concernant les cultures transgeniques de deuxieme generation restent cependant sans reponses. Les changements climatiques qui affectent les zones d’adaptabilite et la croissance des cultures modifient aussi celles des plantes nuisibles. Des adventices notoires beneficient deja de l’accroissement des concentrations atmospheriques d’ozone et de CO2. En contrepartie, des especes nuisibles sont pressenties comme sources de biocarburants et de nouvelles cultures malgre une connaissance incomplete des consequences de leur mise en culture. On tente aussi de reconnaitre la contribution de certaines mauvaises herbes a la biodiversite malgre une comprehension fort partielle des relations entre les mauvaises herbes et les autres organismes de l’ecosysteme agricole. La connaissance des plantes « nuisibles » revet plus que jamais une importance strategique, ces plantes etant au coeur de secteurs nevralgiques, economiques et environnementaux.
本文的目的是确定许多当代农业和其他问题的草药维度,矛盾的是,这一学科的科学和技术资源正变得越来越稀缺。最近采用的抗除草剂转基因作物有助于确定与基因流动和转基因在环境中的持久性有关的农业生态问题。然而,关于第二代转基因作物的问题仍然没有答案。影响作物适应性和生长的气候变化也会影响害虫的适应性和生长。众所周知的外来物种已经受益于大气中臭氧和二氧化碳浓度的增加。另一方面,有害物种被认为是生物燃料和新作物的来源,尽管对其种植的后果知之甚少。人们还试图承认某些杂草对生物多样性的贡献,尽管对杂草和农业生态系统中其他生物之间的关系知之甚少。了解“有害”植物比以往任何时候都具有战略重要性,因为这些植物是敏感、经济和环境部门的核心。
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引用次数: 1
Note du rédacteur en chef / Editor’s Note 编辑的说明/编辑的说明
4区 农林科学 Q4 PLANT SCIENCES Pub Date : 2010-01-01 DOI: 10.7202/1008538ar
D. Dostaler
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引用次数: 0
« Un siècle de pathologie forestière au Québec, sur fond de grands pins » “魁北克一个世纪的森林病理学,在大松树的背景下”
4区 农林科学 Q4 PLANT SCIENCES Pub Date : 2009-11-20 DOI: 10.7202/038233AR
G. Laflamme
A l’occasion du centenaire de la Societe de protection des plantes du Quebec, nous regardons le chemin parcouru par les pathologistes forestiers de cette province. Pour chacun d’eux, nous soulignons des faits saillants de leur carriere. Durant ce siecle, nous reconnaissons trois periodes, soit celle des precurseurs, celle de l’âge d’or, qui debute avec Rene Pomerleau et qui a vu passer le plus grand nombre de pathologistes forestiers, et enfin celle dite de la turbulence que nous vivons actuellement. Nous terminons avec les perspectives d’avenir qui peuvent paraitre un peu sombres. Cependant, compte tenu de la realite de l’etat de sante des forets, l’avenir est en realite plutot prometteur.
在魁北克植物保护协会成立100周年之际,我们回顾了该省森林病理学家所走过的道路。对于他们每个人,我们都强调他们职业生涯中的亮点。在这个世纪里,我们认识到三个时期:先驱者时期,黄金时代,从勒内·波默洛开始,见证了大量的森林病理学家的到来,最后是所谓的动荡时期,我们现在正在经历。我们以未来的前景结束,这可能看起来有点黯淡。然而,考虑到森林健康状况的现实,未来是相当光明的。
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引用次数: 1
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Phytoprotection
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