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Change of pore water near the freezing front during soil freezing: Migration and mechanisms 冻结锋附近孔隙水在土壤冻结过程中的变化:迁移与机制
IF 5.2 2区 农林科学 Q1 SOIL SCIENCE Pub Date : 2024-08-01 DOI: 10.1016/j.pedsph.2023.06.009

The formation and growth of segregation ice dominate the frost heave during soil freezing, which is closely related to water migration. To analyze segregation ice during soil freezing for different soils, a freezing-thawing experiment was conducted with Lanzhou loess (LZL) and Qinghai-Tibet Plateau silty clay (QSC) using a novel layered nuclear magnetic resonance approach. During LZL freezing, the unfrozen water content first increased and then decreased with decreasing temperature near the freezing front, but decreased with decreasing temperature in other layers, whereas during the freezing of QSC, the unfrozen water content in different layers (including the freezing front) decreased with decreased temperature. Notably, the increased liquid water near the freezing front during LZL freezing was primarily adsorbed water. In addition to the temperature gradient, the squeezing action during soil freezing was another important factor affecting water migration, especially at the early stage of soil freezing. However, which of the two factors, squeezing action and temperature gradient, was the dominant one causing water migration depended on soil structure. At the early stage of soil freezing, the squeezing action had a significant effect on the water migration of LZL, but no significant effect on that of QSC. Furthermore, water accumulation of LZL near the freezing front due to squeezing action at the early stage of freezing limited the formation and growth of segregation ice. This study provided an improved understanding for ice segregation and water migration during soil freezing.

土壤冻结过程中,离析冰的形成和生长主导着冻土起伏,与水分迁移密切相关。为了分析不同土壤冻结过程中的离析冰,采用新型分层核磁共振方法对兰州黄土(LZL)和青藏高原淤泥质粘土(QSC)进行了冻融实验。在 LZL 冻结过程中,随着温度的降低,冻结前沿附近的未冻结水含量先增加后减少,但其他层的未冻结水含量则随着温度的降低而减少;而在 QSC 冻结过程中,不同层(包括冻结前沿)的未冻结水含量则随着温度的降低而减少。值得注意的是,LZL冻结过程中冻结前沿附近增加的液态水主要是吸附水。除温度梯度外,土壤冻结过程中的挤压作用也是影响水分迁移的另一个重要因素,尤其是在土壤冻结的早期阶段。然而,挤压作用和温度梯度这两个因素中哪个是导致水分迁移的主要因素取决于土壤结构。在土壤冻结初期,挤压作用对 LZL 的水分迁移有显著影响,但对 QSC 的水分迁移没有显著影响。此外,冻结初期的挤压作用导致 LZL 在冻结前沿附近积水,限制了离析冰的形成和生长。这项研究加深了人们对土壤冻结过程中冰的分离和水的迁移的理解。
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引用次数: 0
Global and regional soil organic carbon estimates: Magnitudes and uncertainties 全球和区域土壤有机碳估算:大小和不确定性
IF 5.2 2区 农林科学 Q1 SOIL SCIENCE Pub Date : 2024-08-01 DOI: 10.1016/j.pedsph.2023.06.005

Globally, soil is the largest terrestrial carbon (C) reservoir. Robust quantification of soil organic C (SOC) stocks in existing global observation-based estimates avails accurate predictions in carbon-climate feedbacks and future climate trends. We investigated the magnitudes and distributions of global and regional SOC estimates (i.e., density and stocks) based on five widely used global gridded SOC datasets, a regional permafrost dataset developed in 2021 (UM2021), and a global-scale soil profile database (World Soil Information Service) reporting measurements of a series of physical and chemical edaphic attributes. The five global gridded SOC datasets were the Harmonized World Soil Database (HWSD), World Inventory of Soil Emission Potentials at 30 arc-second resolution (WISE30sec), Global Soil Dataset for Earth System Models (GSDE), Global Gridded Soil Information at 250-m resolution (SoilGrids250m), and Global Soil Organic Carbon Map (GSOCmap). Our analyses showed that the magnitude and distribution of SOC varied widely among datasets, with certain datasets showing region-specific robustness. At the global scale, SOC stocks at the top 30 and 100 cm were estimated to be 828 (range: 577–1 171) and 1 873 (range: 1 086–2 678) Pg C, respectively. The estimates from GSDE, GSOCmap, and WISE30sec were comparable, and those of SoilGrids250m and HWSD were at the upper and lower ends. The spatial SOC distribution varied greatly among datasets, especially in the northern circumpolar and Tibetan Plateau permafrost regions. Regionally, UM2021 and WISE30sec performed well in the northern circumpolar permafrost regions, and GSDE performed well in China. The estimates of SOC by different datasets also showed large variabilities across different soil layers and biomes. The discrepancies were generally smaller for the 0–30 cm soil than the 0–100 cm soil. The datasets demonstrated relatively higher agreement in grasslands, croplands, and shrublands/savannas than in other biomes (e.g., wetlands). The users should be mindful of the gaps between regions and biomes while choosing the most appropriate SOC dataset for specific uses. Large uncertainties in existing global gridded SOC estimates were generally derived from soil sampling density, different sources, and various mapping methods for soil datasets. We call for future efforts for standardizing soil sampling efforts, cross-dataset comparison, proper validation, and overall global collaboration to improve SOC estimates.

在全球范围内,土壤是最大的陆地碳库。在现有的基于全球观测的估算中,对土壤有机碳(SOC)储量的精确量化有助于准确预测碳-气候反馈和未来气候趋势。我们基于五个广泛使用的全球网格 SOC 数据集、一个 2021 年开发的区域永久冻土数据集(UM2021)和一个全球尺度土壤剖面数据库(世界土壤信息服务),研究了全球和区域 SOC 估算值(即密度和储量)的大小和分布。五个全球网格化土壤有机碳数据集是:世界土壤统一数据库(HWSD)、30 弧秒分辨率的世界土壤排放潜势清单(WISE30sec)、地球系统模型全球土壤数据集(GSDE)、250 米分辨率的全球网格化土壤信息(SoilGrids250m)和全球土壤有机碳地图(GSOCmap)。我们的分析表明,不同数据集之间的 SOC 量级和分布差异很大,某些数据集显示出特定区域的稳健性。在全球范围内,30 厘米和 100 厘米顶部的 SOC 储量估计分别为 828(范围:577-1 171)和 1 873(范围:1 086-2 678)Pg C。GSDE、GSOCmap 和 WISE30sec 的估算值相当,而 SoilGrids250m 和 HWSD 的估算值处于上下两端。不同数据集的 SOC 空间分布差异很大,尤其是在环北极北部和青藏高原冻土区。从区域来看,UM2021 和 WISE30sec 在北环极圈冻土区表现良好,GSDE 在中国表现良好。不同数据集对 SOC 的估计值在不同土层和生物群落之间也存在较大差异。0-30 厘米土壤的差异一般小于 0-100 厘米土壤。与其他生物群落(如湿地)相比,数据集在草地、耕地和灌木林地/热带草原的一致性相对较高。用户在为特定用途选择最合适的 SOC 数据集时,应注意区域和生物群落之间的差距。现有全球网格 SOC 估计值的巨大不确定性通常来自土壤采样密度、不同来源和土壤数据集的不同制图方法。我们呼吁今后努力实现土壤取样标准化、跨数据集比较、适当验证和全球合作,以改进 SOC 估算值。
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引用次数: 0
In-situ warming does not change soil priming effect induced by glucose addition in a temperate forest 原位增温和添加葡萄糖对温带森林土壤呼吸的影响
IF 5.2 2区 农林科学 Q1 SOIL SCIENCE Pub Date : 2024-08-01 DOI: 10.1016/j.pedsph.2023.06.003

Priming effect (PE) on soil organic carbon (SOC) decomposition caused by the addition of organic carbon (C) is an important ecological process in regulating soil C cycle. Additionally, most priming studies are confined to laboratory trials, while the assessment of soil PE under field conditions with variable weather conditions is scarce. This study assessed the direct effects of glucose addition and in-situ warming on the extent of PE under field conditions in a temperate forest. We evaluated soil PE using 13C-glucose labelling, a simple and novel technique, based on the Keeling plot method. Glucose addition significantly enhanced native SOC decomposition and induced strong PE. However, the effect of in-situ warming on the extent of PE was not significant. This study confirms the importance of PE in regulating SOC turnover under field conditions.

添加有机碳(C)对土壤有机碳(SOC)分解的引诱效应(PE)是调节土壤碳循环的一个重要生态过程。此外,大多数引诱效应研究仅限于实验室试验,而在天气条件多变的野外条件下对土壤引诱效应的评估却很少。本研究评估了在温带森林野外条件下添加葡萄糖和原地升温对土壤PE程度的直接影响。我们使用 13C 葡萄糖标记法评估了土壤 PE,这是一种基于 Keeling plot 法的简单而新颖的技术。葡萄糖的添加明显促进了原生 SOC 的分解,并诱发了强烈的 PE。然而,原地升温对 PE 的影响并不明显。这项研究证实了 PE 在田间条件下调节 SOC 转化的重要性。
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引用次数: 0
Combining conservation tillage with nitrogen fertilization promotes maize straw decomposition by regulating soil microbial community and enzyme activities 保护性耕作配施氮肥措施通过调节微生物群落和酶活性促进玉米秸秆分解
IF 5.2 2区 农林科学 Q1 SOIL SCIENCE Pub Date : 2024-08-01 DOI: 10.1016/j.pedsph.2023.05.005

Straw return can effectively improve farmland soil microenvironment and fertility. However, excessive straw in the topsoil adversely affects seed germination and crop growth. At present, the characteristics and key driving factors of straw decomposition in dry farmlands are unclear. Based on the interactions between tillage practices including zero tillage (ZT), chisel tillage (CT), and plow tillage (PT) and nitrogen (N) fertilization, i.e., low N (N1, 180 kg ha-1), normal N (N2, 240 kg ha-1), and high N (N3, 300 kg ha-1), quantitative polymerase chain reaction technology and an enzymatic detection kit were used to investigate the effects of key straw C-degrading enzyme activities and microbial abundance in soil on maize straw decomposition during the growth period of winter wheat in the winter wheat/summer maize double cropping system in a dry farmland of the Loess Plateau, China. Between 2018 and 2020, ZT and CT significantly increased winter wheat yield (by 10.94% and 12.79%, respectively) and straw decomposition velocity (by 20% and 26.67%, respectively), compared with PT. Compared to N1 and N3, N2 significantly increased wheat yield (by 4.65% and 5.31%, respectively) and straw decomposition velocity (by 26.33% and 13.21%, respectively). The partial least squares pathway modelling showed significant positive direct effects of soil moisture, NO3-, NH4+, total N, bacteria, and cellulase, laccase, and xylanase activities on straw decomposition, while soil pH, fungi, and Actinomycetes had significant negative direct effects. Overall, conservation tillage (ZT and CT) combined with N2 was beneficial for straw decomposition in the drylands of the Loess Plateau and improved straw resource utilization and basic soil fertility. The results of the study clarified the key drivers of straw decomposition in dry farmlands and provided new ideas for developing updated soil management practices and adaptive N application strategies to promote the resource utilization of straw and achieve the goals of carbon peaking and carbon neutrality.

秸秆还田可以有效改善农田土壤微环境和肥力。然而,表层土壤中过多的秸秆会对种子发芽和作物生长产生不利影响。目前,干旱农田中秸秆分解的特征和关键驱动因素尚不清楚。基于零耕(ZT)、凿耕(CT)和犁耕(PT)等耕作方式与氮肥(即采用定量聚合酶链式反应技术和酶学检测试剂盒,研究了中国黄土高原干旱农田冬小麦/夏玉米双季作业系统中,冬小麦生长期关键秸秆C降解酶活性和土壤中微生物丰度对玉米秸秆分解的影响。2018年至2020年间,与PT相比,ZT和CT显著提高了冬小麦产量(分别提高了10.94%和12.79%)和秸秆分解速度(分别提高了20%和26.67%)。与 N1 和 N3 相比,N2 显著提高了小麦产量(分别提高了 4.65% 和 5.31%)和秸秆分解速度(分别提高了 26.33% 和 13.21%)。偏最小二乘法路径模型显示,土壤水分、NO3-、NH4+、全氮、细菌以及纤维素酶、漆酶和木聚糖酶活性对秸秆分解有显著的正向直接影响,而土壤 pH 值、真菌和放线菌对秸秆分解有显著的负向直接影响。总之,保护性耕作(ZT 和 CT)与氮磷结合有利于黄土高原旱地的秸秆分解,提高秸秆资源利用率和土壤基础肥力。研究结果阐明了干旱农田秸秆分解的关键驱动因素,为制定更新的土壤管理措施和适应性氮施用策略提供了新思路,从而促进秸秆资源化利用,实现碳峰值和碳中和目标。
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引用次数: 0
Fate of C and N from pretreated organic amendments in soil 预处理过的有机添加剂在土壤中的碳和氮的归宿
IF 7.3 2区 农林科学 Q1 SOIL SCIENCE Pub Date : 2024-07-19 DOI: 10.1016/j.pedsph.2024.07.007
Vania Scarlet CHAVEZ-RICO , Paul L.E. BODELIER , Miriam H.A. VAN EEKERT , Valentina SECHI , Valeriu CIOCAN , Eline KEUNING , Noé MALAGON-MENDOZA , Dominic RINGLER , Cees BUISMAN
Soil organic amendments (OAs) are used to replenish carbon (C) and nutrients in the soil to prevent its degradation and increase its fertility. While soil can be an important C sink, it can also release significant amounts of greenhouse gases (GHGs). Different OA pretreatment technologies indirectly affect soil aggregate formation and C stabilization even when the same initial substrate is used. However, little is known about the long-term effect of OA pretreatment on the soil C and nitrogen (N) associated with macroaggregates, which are known to disintegrate faster than microaggregates. In this study, we studied the effect of OA pretreatment on soil C and N in relation to aggregate formation and GHG emissions using five differently pretreated OAs from the same original OA, i.e., composted, digested, and fermented OA, a 1:1 mix of the composted and fermented OAs, and the unpretreated original OA. We monitored the changes in a soil column experiment after 6 and 12 months of incubation. Our results indicated that OA pretreatment indirectly affected GHG emissions from soil. The composted and mixed OAs released less GHGs (i.e., carbon dioxide, nitrous oxide, and methane) but had no positive impact on macroaggregates, while the digested OA induced long-lasting macroaggregation and occluded particulate organic matter formation, emitting intermediate levels of GHGs. The unpretreated OA exhibited the highest GHG emissions, similar to the fermented OA, albeit without benefiting macroaggregation. These demonstrated that OA pretreatment had a long-lasting indirect effect on soil C and N, influencing total GHG emissions, nitrous oxide formation mechanisms, and soil macroaggregate formation.
土壤有机改良剂(OAs)用于补充土壤中的碳(C)和养分,以防止其退化并提高其肥力。虽然土壤是一个重要的碳汇,但它也可以释放大量的温室气体(ghg)。不同的OA预处理技术,即使使用相同的初始基质,也会间接影响土壤团聚体的形成和碳的稳定。然而,人们对OA预处理对土壤中与大团聚体相关的碳和氮(N)的长期影响知之甚少,大团聚体比微团聚体崩解得更快。在这项研究中,我们研究了OA预处理对土壤C和N与团聚体形成和温室气体排放的影响,使用来自同一原始OA的5种不同预处理的OA,即堆肥、消化和发酵OA,堆肥和发酵OA的1:1混合,以及未经预处理的原始OA。在6个月和12个月的孵育后,我们在土柱实验中监测了变化。结果表明,OA预处理对土壤温室气体排放有间接影响。堆肥和混合OA释放的温室气体(即二氧化碳、氧化亚氮和甲烷)较少,但对大团聚体没有积极影响,而消化的OA诱导了长期的大团聚体并阻塞了颗粒有机物的形成,排放中等水平的温室气体。未经预处理的OA表现出最高的温室气体排放,与发酵的OA相似,尽管没有受益于宏观聚集。这些结果表明,OA预处理对土壤C和N具有长期的间接影响,影响温室气体排放总量、氧化亚氮形成机制和土壤大团聚体形成。
{"title":"Fate of C and N from pretreated organic amendments in soil","authors":"Vania Scarlet CHAVEZ-RICO ,&nbsp;Paul L.E. BODELIER ,&nbsp;Miriam H.A. VAN EEKERT ,&nbsp;Valentina SECHI ,&nbsp;Valeriu CIOCAN ,&nbsp;Eline KEUNING ,&nbsp;Noé MALAGON-MENDOZA ,&nbsp;Dominic RINGLER ,&nbsp;Cees BUISMAN","doi":"10.1016/j.pedsph.2024.07.007","DOIUrl":"10.1016/j.pedsph.2024.07.007","url":null,"abstract":"<div><div>Soil organic amendments (OAs) are used to replenish carbon (C) and nutrients in the soil to prevent its degradation and increase its fertility. While soil can be an important C sink, it can also release significant amounts of greenhouse gases (GHGs). Different OA pretreatment technologies indirectly affect soil aggregate formation and C stabilization even when the same initial substrate is used. However, little is known about the long-term effect of OA pretreatment on the soil C and nitrogen (N) associated with macroaggregates, which are known to disintegrate faster than microaggregates. In this study, we studied the effect of OA pretreatment on soil C and N in relation to aggregate formation and GHG emissions using five differently pretreated OAs from the same original OA, <em>i.e</em>., composted, digested, and fermented OA, a 1:1 mix of the composted and fermented OAs, and the unpretreated original OA. We monitored the changes in a soil column experiment after 6 and 12 months of incubation. Our results indicated that OA pretreatment indirectly affected GHG emissions from soil. The composted and mixed OAs released less GHGs (<em>i.e</em>., carbon dioxide, nitrous oxide, and methane) but had no positive impact on macroaggregates, while the digested OA induced long-lasting macroaggregation and occluded particulate organic matter formation, emitting intermediate levels of GHGs. The unpretreated OA exhibited the highest GHG emissions, similar to the fermented OA, albeit without benefiting macroaggregation. These demonstrated that OA pretreatment had a long-lasting indirect effect on soil C and N, influencing total GHG emissions, nitrous oxide formation mechanisms, and soil macroaggregate formation.</div></div>","PeriodicalId":49709,"journal":{"name":"Pedosphere","volume":"35 6","pages":"Pages 957-970"},"PeriodicalIF":7.3,"publicationDate":"2024-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141846879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of sulfur fertilizers applied at agronomic rates on cadmium availability in agricultural soils: Insights from a batch experiment 按农艺比例施用硫肥对农业土壤中镉含量的影响:分批实验的启示
IF 7.3 2区 农林科学 Q1 SOIL SCIENCE Pub Date : 2024-07-19 DOI: 10.1016/j.pedsph.2024.07.009
Agathe VIDAL, Christophe NGUYEN, Noémie JANOT, Pierre EON, Cécile CORIOU, Jean-Yves CORNU
Cadmium (Cd) is a toxic trace metal that occurs naturally in agricultural soils and can accumulate in the edible parts of crops, notably in cereal grains. This study investigated the extent to which sulfur (S) fertilization increases the availability of Cd in a calcareous (Calc) and a non-calcareous (Ncalc) soil. Changes in Cd availability and speciation were monitored over a 21-d incubation period, along with changes in pH and the concentrations of sulfate, nitrate, calcium, magnesium, aluminum (Al), manganese (Mn), and nickel (Ni) in the potassium nitrate (KNO3) extracts, after the addition at agronomic rates of three forms of S fertilizers: elemental S (S(0)), potassium sulfate (K2SO4), and ammonium sulfate ((NH4)2SO4). Elemental S did not increase the Cd concentration in the KNO3 extracts of either soil due to its too limited oxidation to sulfates. The two sulfate fertilizers had contrasting effects on Cd concentration depending on the nature of the counter cation (K+ or NH4+). While K2SO4 caused almost no change in Cd concentration in either soil, (NH4)2SO4 increased Cd concentration in both soils. The nitrification of NH4+ reduced soil pH, thereby increasing the concentrations of Cd, Al, Mn, and Ni in the Ncalc soil over time, as evidenced by the strong negative correlations between soil pH and the concentrations of these four elements in the KNO3 extract after 21 d. In the Calc soil, the mechanism of Cd concentration increase after (NH4)2SO4 addition was more difficult to understand and could result from the proton-mediated alteration of Cd-bearing phases such as carbonates, following ammonia (NH3) volatilization. The relevance of these results for the S fertilization of cereals in Cd-contaminated soils is discussed.
镉(Cd)是一种有毒的微量金属,自然存在于农业土壤中,可积聚在作物的可食用部分,特别是谷物中。本研究探讨了硫(S)施肥在多大程度上增加了钙(Calc)和非钙(Ncalc)土壤中Cd的有效性。以农艺速率添加三种形式的S肥料:单质S(S(0))、硫酸钾(K2SO4)和硫酸铵(NH4)2SO4)后,在21天的孵育期内监测Cd有效性和形态的变化,以及硝酸钾(KNO3)提取物中pH和硫酸盐、硝酸盐、钙、镁、铝(Al)、锰(Mn)和镍(Ni)浓度的变化。元素S没有增加土壤KNO3提取物中Cd的浓度,这是因为元素S对硫酸盐的氧化作用太有限。根据反阳离子(K+或NH4+)的性质,两种硫酸盐肥料对Cd浓度的影响不同。K2SO4对两种土壤的Cd浓度几乎没有影响,而(NH4)2SO4使两种土壤的Cd浓度升高。硝化NH4 +土壤pH值降低,从而增加浓度的Cd,铝、锰、和倪Ncalc土壤随着时间的推移,土壤pH值之间就是明证强负相关性,这四种元素的浓度在21 d后硝酸钾工艺提取。钙土,Cd浓度增加后的机制(NH4) 2 so4之外更难以理解和可能导致从专辑的由蚀变碳酸盐等阶段,随着氨(NH3)的挥发。讨论了这些结果与镉污染土壤中谷物施S肥的相关性。
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引用次数: 0
Brackish water irrigation-induced recruitment of specific consortium determines microbial resistance that facilitates crop yield in a field experiment 在一项田间试验中,由咸水灌溉引起的特定菌群招募决定了微生物的抗性,从而提高了作物产量
IF 7.3 2区 农林科学 Q1 SOIL SCIENCE Pub Date : 2024-07-19 DOI: 10.1016/j.pedsph.2024.07.005
Qingxia WANG , Jisheng XU , Dandan LI , Yunpeng ZHOU , Meiqi CHEN , Xiying ZHANG , Jiabao ZHANG , Bingzi ZHAO
Crop productivity may be affected by soil salinization from brackish water irrigation. Plants host species-specific soil microorganisms that can enhance plant adaptability to salt stress. However, it is unclear whether these specific microorganisms recruited under brackish water irrigation are related to microbial resistance, which has been proven to promote crop production. A field experiment was conducted using five local wheat varieties, each of which was exposed to brackish or fresh water irrigation for four years. Brackish water irrigation tended to increase wheat yield by 6.38%–19.40%, although the increase in yield under brackish water relative to fresh water irrigation varied with wheat variety. The compositions of the bacterial and fungal communities in the bulk soil and rhizosphere were measured, and the taxa enriched following brackish water irrigation were isolated to investigate microbial resistance. We found that the resistance of each wheat variety was determined by specifically recruited microbial taxa with relevant functions. The yield incremental rates were positively regulated by microbial resistance from the bulk soil and rhizosphere. Moreover, the resistance from the bulk soil and rhizosphere had similar effects, and microbial consortia containing both beneficial and harmful taxa determined microbial resistance. These results indicated that brackish water irrigation-induced recruitment of specific microbial taxa from either the bulk soil or the rhizosphere or both positively contributed to the yield incremental rate and provided a list of key taxa linked to the magnitude of yield variation caused by brackish water irrigation.
由于微咸水灌溉造成的土壤盐碱化可能影响作物生产力。植物寄主物种特异性土壤微生物,可以增强植物对盐胁迫的适应性。然而,目前尚不清楚在微咸水灌溉下招募的这些特定微生物是否与微生物抗性有关,而微生物抗性已被证明可以促进作物生产。以5个地方小麦品种为研究对象,分别进行了4年的微咸水或淡水灌溉试验。微咸水灌溉有利于小麦增产6.38% ~ 19.40%,但微咸水灌溉相对于淡水灌溉的增产幅度因小麦品种而异。测定了块状土壤和根际细菌和真菌群落的组成,并分离了微咸水灌溉后富集的类群,研究了微生物的耐药性。我们发现每个小麦品种的抗性是由特定招募的具有相关功能的微生物类群决定的。块状土壤和根际微生物的抗性正调控着产量的增加速率。此外,土壤和根际的抗性具有相似的效应,并且含有有益和有害类群的微生物群落决定了微生物的抗性。这些结果表明,微咸水灌溉诱导了块状土壤或根际或两者的特定微生物类群的补充,对产量增量率有正贡献,并提供了与微咸水灌溉引起的产量变化幅度相关的关键类群列表。
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引用次数: 0
Co-inoculation of protist and Bacillus enhances plant growth via reshaping rhizosphere bacterial community composition and function 联合应用原生动物和芽孢杆菌,通过重塑根瘤菌的组成和功能促进植物生长
IF 7.3 2区 农林科学 Q1 SOIL SCIENCE Pub Date : 2024-07-19 DOI: 10.1016/j.pedsph.2024.07.006
Qihui LÜ , Chen LIU , Ying GUI , Yang YUE , Xiao WANG , Zeyuan ZHOU , Ying YUAN , Yijing WANG , Boting XU , Zhihui XU , Mohammadhossein RAVANBAKHSH , Alexandre JOUSSET , Wu XIONG , Qirong SHEN
Plant growth-promoting rhizobacteria (PGPR) have been widely used for the promotion of plant performance. Predatory protists can influence the taxonomic and functional composition of rhizosphere bacteria. However, research on the impact of the interaction between protist and PGPR on plant performance remains at a very early stage. Here, we examined the impacts of individual inoculation of protist (Colpoda inflata, Dimastigella trypaniformis, or Vermamoeba vermiformis) or the PGPR strain Bacillus velezensis SQR9 as well as the co-inoculation of the protist C. inflata and B. velezensis SQR9 on the growth of tomato plants. We found that all individual protists and Bacillus could promote plant growth compared to the control with no microbe inoculation, with the co-inoculation of C. inflata and B. velezensis SQR9 achieving the greatest performance, including plant height, fresh weight, and dry weight. Different protists harbored distinct rhizosphere bacterial communities, with the co-inoculation of protist and Bacillus resulting in the lowest bacterial diversity and driving significant changes in community structure and composition, particularly by increasing the relative abundance of Proteobacteria. Random forest model highlighted Cellvibrio as the most important bacterial predictor of plant growth, which was enriched after protist inoculation, especially after the mixed inoculation of protist and Bacillus. We further found that bacterial functional genes of nitrogen metabolism were the key determinants of plant growth. These results indicate that the interaction between protists and Bacillus can support plant growth by reshaping rhizosphere bacterial community composition and function. Understanding the interaction mechanisms between protist and PGPR is crucial for their effective utilization in sustainable agriculture.
植物促生根瘤菌(PGPR)被广泛用于促进植物生长性能。掠夺性原生生物可以影响根际细菌的分类和功能组成。然而,原生生物与PGPR相互作用对植物生产性能影响的研究仍处于非常早期的阶段。本研究研究了单独接种原生生物(膨胀Colpoda, Dimastigella trypaniformis, or Vermamoeba vermiformis)或PGPR菌株velezensis SQR9,以及膨胀原生生物C.和velezensis SQR9共同接种对番茄植株生长的影响。结果表明,与不接种微生物的对照相比,所有原生生物和芽孢杆菌均能促进植株的生长,其中胀孢杆菌和velezensis SQR9共接种效果最好,包括株高、鲜重和干重。不同的原生生物拥有不同的根际细菌群落,原生生物和芽孢杆菌共接种导致细菌多样性最低,并导致群落结构和组成的显著变化,特别是通过增加Proteobacteria的相对丰度。随机森林模型强调Cellvibrio是植物生长最重要的细菌预测因子,在原生生物接种后,特别是在原生生物与芽孢杆菌混合接种后,其数量显著增加。我们进一步发现细菌氮代谢功能基因是植物生长的关键决定因素。这些结果表明,原生生物与芽孢杆菌的相互作用可以通过重塑根际细菌群落组成和功能来支持植物的生长。了解原生生物与PGPR的相互作用机制对其在可持续农业中的有效利用至关重要。
{"title":"Co-inoculation of protist and Bacillus enhances plant growth via reshaping rhizosphere bacterial community composition and function","authors":"Qihui LÜ ,&nbsp;Chen LIU ,&nbsp;Ying GUI ,&nbsp;Yang YUE ,&nbsp;Xiao WANG ,&nbsp;Zeyuan ZHOU ,&nbsp;Ying YUAN ,&nbsp;Yijing WANG ,&nbsp;Boting XU ,&nbsp;Zhihui XU ,&nbsp;Mohammadhossein RAVANBAKHSH ,&nbsp;Alexandre JOUSSET ,&nbsp;Wu XIONG ,&nbsp;Qirong SHEN","doi":"10.1016/j.pedsph.2024.07.006","DOIUrl":"10.1016/j.pedsph.2024.07.006","url":null,"abstract":"<div><div>Plant growth-promoting rhizobacteria (PGPR) have been widely used for the promotion of plant performance. Predatory protists can influence the taxonomic and functional composition of rhizosphere bacteria. However, research on the impact of the interaction between protist and PGPR on plant performance remains at a very early stage. Here, we examined the impacts of individual inoculation of protist (<em>Colpoda inflata</em>, <em>Dimastigella trypaniformis</em>, or <em>Vermamoeba vermiformis</em>) or the PGPR strain <em>Bacillus velezensis</em> SQR9 as well as the co-inoculation of the protist <em>C. inflata</em> and <em>B. velezensis</em> SQR9 on the growth of tomato plants. We found that all individual protists and <em>Bacillus</em> could promote plant growth compared to the control with no microbe inoculation, with the co-inoculation of <em>C. inflata</em> and <em>B. velezensis</em> SQR9 achieving the greatest performance, including plant height, fresh weight, and dry weight. Different protists harbored distinct rhizosphere bacterial communities, with the co-inoculation of protist and <em>Bacillus</em> resulting in the lowest bacterial diversity and driving significant changes in community structure and composition, particularly by increasing the relative abundance of Proteobacteria. Random forest model highlighted <em>Cellvibrio</em> as the most important bacterial predictor of plant growth, which was enriched after protist inoculation, especially after the mixed inoculation of protist and <em>Bacillus</em>. We further found that bacterial functional genes of nitrogen metabolism were the key determinants of plant growth. These results indicate that the interaction between protists and <em>Bacillus</em> can support plant growth by reshaping rhizosphere bacterial community composition and function. Understanding the interaction mechanisms between protist and PGPR is crucial for their effective utilization in sustainable agriculture.</div></div>","PeriodicalId":49709,"journal":{"name":"Pedosphere","volume":"35 5","pages":"Pages 893-900"},"PeriodicalIF":7.3,"publicationDate":"2024-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141840106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
An evaluation approach integrating remedial duration for sustainable mega-site remediation in China 中国超大型场地可持续修复与修复期限相结合的评估方法
IF 7.3 2区 农林科学 Q1 SOIL SCIENCE Pub Date : 2024-07-19 DOI: 10.1016/j.pedsph.2024.07.008
Xin LIU , Peng JIN , Zongshuai YANG , Changlong WEI , Paul BARDOS , Da DING , Zhuanxia ZHANG , Xin SONG
Due to the fast-paced redevelopment of contaminated sites in developing countries, there is a lack of appropriate quantitative approaches to evaluate sustainability for the selection of remedial alternatives. In this study, an integrated evaluation approach for sustainability assessment was developed and applied to the site remediation of a former large-scale iron and steel manufacturing facility in China. This approach incorporated remedial duration as a distinct tier within a multi-criteria decision analysis framework. The traditional environmental, economic, and social assessments, consisting of 15 categories and 50 indicators for no action alternative, one in situ alternative, and three ex situ alternatives, were performed as the first-tier evaluation, which was then integrated with the second-tier evaluation on the remedial duration. The findings revealed that advanced in situ remediation was associated with a long duration of remedial activities, preventing stakeholders from attaining timely benefits after successful land redevelopment. Furthermore, the evaluation of sustainable remediation was correlated with sustainable development goals (SDG), supporting the conclusion that on-site/off-site treatment alternative performed well in both sustainability and timely redevelopment demands. The scoring of alternatives was further validated through uncertainty analysis, which demonstrated that the on-site/off-site treatment alternative had the highest probability of attaining the best sustainability performance. In addition, sensitivity analysis revealed that remedial duration had the strongest correlation and sensitivity in the integrated evaluation. By integrating SDG targets with the concept of sustainable remediation, this study promotes the greatest net benefit from implementing a sustainable and resilient remediation strategy. Practically, this study proposed an integrated model to promote sustainable remediation outcomes for contaminated sites in fast-developing countries and markets, while considering the constraints of remedial duration and the pursuit of ecological functions.
由于发展中国家受污染场地的快速重建,缺乏适当的定量方法来评价选择补救办法的可持续性。本研究提出了一种可持续性评价的综合评价方法,并将其应用于中国某原大型钢铁生产设施的场地修复中。这种方法将补救持续时间作为多标准决策分析框架中的一个不同层次。传统的环境、经济和社会评价包括15个类别和50个指标,包括无行动替代方案、1个原地替代方案和3个迁地替代方案,作为第一级评价,然后与关于补救持续时间的第二级评价相结合。研究结果显示,先进的原地修复与修复活动持续时间较长有关,阻碍了利益相关者在成功的土地重建后及时获得利益。此外,可持续修复的评价与可持续发展目标(SDG)相关,支持现场/非现场处理方案在可持续性和及时重建需求方面都表现良好的结论。通过不确定性分析进一步验证了备选方案的得分,结果表明,现场/非现场处理方案获得最佳可持续性绩效的概率最高。敏感性分析显示,综合评价中,治疗时间的相关性和敏感性最强。通过将可持续发展目标目标与可持续修复概念相结合,本研究促进了实施可持续和有弹性的修复战略所带来的最大净效益。在实践中,本研究提出了一个综合模型,以促进快速发展国家和市场中污染场地的可持续修复结果,同时考虑修复时间和生态功能追求的约束。
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引用次数: 0
Improving Cd and Zn enrichment in leaf mustard (Brassica juncea) on heavy metal-contaminated soil using metal-activating probiotics 利用金属活化益生菌提高叶用芥菜(Brassica juncea)中镉和锌的富集度
IF 7.3 2区 农林科学 Q1 SOIL SCIENCE Pub Date : 2024-07-04 DOI: 10.1016/j.pedsph.2024.07.002
Qian LI , Ping WANG , Yiman LI , Zhaowen YANG , Xu YANG , Kailin LIANG , Yizhuo LIU , Zehua WEN , Shuqiang ZHANG , Zengqiang ZHANG
Microbe-assisted phytoremediation is of great significance for the remediation of soil contaminated with heavy metals (HMs), and probiotics are beneficial microorganisms that can improve soil structure and fertility and promote plant growth. However, there are few studies on probiotics applied to remediate soil contaminated with HMs, and whether probiotics can improve the efficiency of phytoremediation still needs to be further investigated. This study aimed to investigate the effects of two kinds of probiotics, Lactobacillus casei (Lc) and Bacillus licheniformis (Bl), on activating the remediation potential of leaf mustard, Brassica juncea (L.) Czerniak., for soil contaminated with Cd and Zn using incubation and pot experiments. The results showed that the addition of the two probiotics significantly reduced soil pH by 0.05–0.32 units and improved the available contents of soil HMs (by 15.3%–60.0% and 7.1%–23.8% for Cd and Zn, respectively) in the incubation experiment. After probiotic addition, available Cd and Zn contents in soil treated with 1×109 colony forming units (cfu) mL-1 Bl were 1.65- and 1.66-folds of those in the control without probiotic, respectively, in the pot experiment. Meanwhile, soil alkaline phosphatase, urease, and sucrose activities were increased, indicating that soil microbial metabolic activities were also stimulated. Addition of Lc and Bl significantly improved the biomass and chlorophyll contents of leaf mustard. The contents of Cd and Zn in shoots and roots were significantly increased in the treatment with 1×105 cfu mL-1 Lc. Furthermore, the activities of plant antioxidant enzymes, including superoxide dismutase, peroxidase, and catalase, were increased, and the content of plant malondialdehyde was reduced, indicating that the resistance of plants to HMs was enhanced. These results indicated that these two kinds of probiotics could enhance the availability of Cd and Zn directly in soil and promote the growth of leaf mustard, thereby increasing the efficiency of phytoremediation for HMs. The study provides a useful reference for probiotic-assisted phytoremediation of soil contaminated with HMs.
微生物辅助植物修复对重金属污染土壤的修复具有重要意义,而益生菌是改善土壤结构和肥力、促进植物生长的有益微生物。然而,益生菌用于修复土壤污染的研究较少,益生菌是否能提高植物修复效率还有待进一步研究。本研究旨在探讨干酪乳杆菌(Lactobacillus casei, Lc)和地衣芽孢杆菌(Bacillus licheniformis, Bl)两种益生菌对芥菜(Brassica juncea, L.)修复潜能的激活作用。Czerniak。对镉、锌污染土壤进行了培养和盆栽试验。结果表明,在培养试验中,两种益生菌的添加显著降低了土壤pH值0.05 ~ 0.32个单位,提高了土壤有机质有效含量(Cd和Zn分别提高15.3% ~ 60.0%和7.1% ~ 23.8%)。在盆栽试验中,添加益生菌后,1×109菌落形成单位(cfu) mL-1 Bl处理的土壤有效镉和有效锌含量分别是未添加益生菌对照的1.65倍和1.66倍。同时,土壤碱性磷酸酶、脲酶和蔗糖活性升高,表明土壤微生物代谢活性也受到刺激。添加Lc和Bl显著提高了芥菜的生物量和叶绿素含量。1×105 cfu mL-1 Lc处理显著提高了地上部和根部Cd、Zn含量。此外,植物超氧化物歧化酶、过氧化物酶和过氧化氢酶等抗氧化酶活性升高,丙二醛含量降低,表明植物对HMs的抗性增强。上述结果表明,这两种益生菌可以直接提高土壤中Cd和Zn的有效性,促进芥菜的生长,从而提高植物对HMs的修复效率。该研究为益生菌辅助植物修复土壤污染提供了有益的参考。
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Pedosphere
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