Pub Date : 2026-03-01Epub Date: 2026-02-06DOI: 10.1016/j.pedobi.2026.151118
Dauren Kaliaskar , Edward W. Bork , Cameron N. Carlyle , Bharat M. Shrestha , Timm F. Döbert , Cole D. Gross , Upama Khatri-Chhetri , Mark S. Boyce , Scott X. Chang
Grazing is a widely practiced land-use activity in grasslands that can influence carbon (C) cycling and nutrient mineralization. In this study, we investigated the effect of grazing treatments, either adaptive multi-paddock (AMP) rotational grazing or conventional grazing (n-AMP), on soil biogeochemical cycling by examining extracellular enzyme activity (EEA), an indicator of soil biological activity. We analyzed the activities of six soil extracellular enzymes involved in C (xylosidase, β-glucosidase, cellobiosidase), nitrogen (N) (N-acetyl-β-glucosaminidase, urease), and phosphorus (P) (phosphatase) cycling. Soil samples were collected from 12 pairs of grasslands, where one of each pair was subjected to long-term AMP grazing, and the adjacent area to conventional grazing. We found that AMP grazing increased the activity of β-glucosidase and phosphatase, which are involved in soil organic matter decomposition and C and P cycling. Positive associations were identified between several independent variables, including rest-to-graze ratio, annual heat-moisture index, soil pH, moisture, and available soil N, with β-glucosidase and phosphatase. In addition to the overall effects of grazing treatment on EEA, extended rest periods between grazing events specifically increased enzyme activities associated with C and P cycling, suggesting that grazing management practices alter key soil biogeochemical processes.
{"title":"Extracellular enzyme activities under adaptive multi-paddock and conventional grazing in grassland soils of western Canada","authors":"Dauren Kaliaskar , Edward W. Bork , Cameron N. Carlyle , Bharat M. Shrestha , Timm F. Döbert , Cole D. Gross , Upama Khatri-Chhetri , Mark S. Boyce , Scott X. Chang","doi":"10.1016/j.pedobi.2026.151118","DOIUrl":"10.1016/j.pedobi.2026.151118","url":null,"abstract":"<div><div>Grazing is a widely practiced land-use activity in grasslands that can influence carbon (C) cycling and nutrient mineralization. In this study, we investigated the effect of grazing treatments, either adaptive multi-paddock (AMP) rotational grazing or conventional grazing (n-AMP), on soil biogeochemical cycling by examining extracellular enzyme activity (EEA), an indicator of soil biological activity. We analyzed the activities of six soil extracellular enzymes involved in C (xylosidase, β-glucosidase, cellobiosidase), nitrogen (N) (N-acetyl-β-glucosaminidase, urease), and phosphorus (P) (phosphatase) cycling. Soil samples were collected from 12 pairs of grasslands, where one of each pair was subjected to long-term AMP grazing, and the adjacent area to conventional grazing. We found that AMP grazing increased the activity of β-glucosidase and phosphatase, which are involved in soil organic matter decomposition and C and P cycling. Positive associations were identified between several independent variables, including rest-to-graze ratio, annual heat-moisture index, soil pH, moisture, and available soil N, with β-glucosidase and phosphatase. In addition to the overall effects of grazing treatment on EEA, extended rest periods between grazing events specifically increased enzyme activities associated with C and P cycling, suggesting that grazing management practices alter key soil biogeochemical processes.</div></div>","PeriodicalId":49711,"journal":{"name":"Pedobiologia","volume":"114 ","pages":"Article 151118"},"PeriodicalIF":2.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146173737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Soil extracellular enzyme stoichiometry serves as a powerful indicator of carbon (C), nitrogen (N), and phosphorus (P) limitations in microbial metabolism. However, how these limitations vary along elevational gradients remains poorly understood. In this study, we analyzed the activities of C-acquiring (β-glucosidase, BG), N-acquiring (N-acetyl-β-glucosaminidase, NAG; leucine aminopeptidase, LAP), and P-acquiring (acid phosphatase, AP) enzymes across five elevations along a mountainous transect. We examined the variations in extracellular enzyme activities, enzyme stoichiometry, and microbial metabolic limitations, and assessed their relationships with key abiotic and biotic factors. The mean enzyme activity ratio (BG:NAG+LAP:AP) was 1:0.36:2.53, deviating from the global stoichiometric mean and indicating a potential nutrient supply imbalance. Vector analysis further suggested that soil microorganisms were predominantly co-limited by C and P, but not by N, across the elevational gradient. The strength of these limitations exhibited nonlinear altitudinal patterns: C limitation was strongest at the lowest and highest elevations, while P limitation peaked in mid-elevation coniferous and mixed forests. Variations in C and P metabolism were primarily driven by vegetation indices, soil properties, and soil CNP content. These findings clarify the altitudinal patterns and drivers of microbial nutrient limitation in subtropical montane forest ecosystems, providing insights into soil microbial functioning that can support strategies for ecological conservation.
土壤胞外酶化学计量学是微生物代谢中碳(C)、氮(N)和磷(P)限制的有力指标。然而，这些限制如何沿着海拔梯度变化仍然知之甚少。在这项研究中，我们分析了c -获取（β-葡萄糖苷酶，BG）， n -获取（n -乙酰-β-葡萄糖苷酶，NAG；亮氨酸氨基肽酶，LAP）和p -获取（酸性磷酸酶，AP）酶在山区样带的五个海拔上的活性。我们研究了细胞外酶活性、酶化学计量学和微生物代谢限制的变化，并评估了它们与关键的非生物和生物因素的关系。平均酶活性比（BG:NAG+LAP:AP）为1:0.36:2.53，偏离了全球化学计量平均值，表明潜在的营养供应失衡。媒介分析进一步表明，在海拔梯度上，土壤微生物主要受C和P的共同限制，而不受N的共同限制。这些限制的强度表现出非线性的垂直分布格局：最低海拔和最高海拔对碳的限制最强，而中海拔针叶林和混交林对磷的限制最大。C和P代谢变化主要受植被指数、土壤性质和土壤CNP含量驱动。这些发现阐明了亚热带山地森林生态系统中微生物养分限制的海拔格局和驱动因素，为土壤微生物功能提供了见解，可以支持生态保护策略。
{"title":"Nutrient limitations revealed by enzyme stoichiometry in soil microbial metabolism across elevational gradients of a subtropical montane ecosystem","authors":"Taotao Wei , Shengen Zhong , Huiguang Zhang , Yirong Zhang , Xinghao Tang , Furong Yang , Jianing Guo , Xin Qian","doi":"10.1016/j.pedobi.2026.151113","DOIUrl":"10.1016/j.pedobi.2026.151113","url":null,"abstract":"<div><div>Soil extracellular enzyme stoichiometry serves as a powerful indicator of carbon (C), nitrogen (N), and phosphorus (P) limitations in microbial metabolism. However, how these limitations vary along elevational gradients remains poorly understood. In this study, we analyzed the activities of C-acquiring (β-glucosidase, BG), N-acquiring (N-acetyl-β-glucosaminidase, NAG; leucine aminopeptidase, LAP), and P-acquiring (acid phosphatase, AP) enzymes across five elevations along a mountainous transect. We examined the variations in extracellular enzyme activities, enzyme stoichiometry, and microbial metabolic limitations, and assessed their relationships with key abiotic and biotic factors. The mean enzyme activity ratio (BG:NAG+LAP:AP) was 1:0.36:2.53, deviating from the global stoichiometric mean and indicating a potential nutrient supply imbalance. Vector analysis further suggested that soil microorganisms were predominantly co-limited by C and P, but not by N, across the elevational gradient. The strength of these limitations exhibited nonlinear altitudinal patterns: C limitation was strongest at the lowest and highest elevations, while P limitation peaked in mid-elevation coniferous and mixed forests. Variations in C and P metabolism were primarily driven by vegetation indices, soil properties, and soil CNP content. These findings clarify the altitudinal patterns and drivers of microbial nutrient limitation in subtropical montane forest ecosystems, providing insights into soil microbial functioning that can support strategies for ecological conservation.</div></div>","PeriodicalId":49711,"journal":{"name":"Pedobiologia","volume":"114 ","pages":"Article 151113"},"PeriodicalIF":2.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146078126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-01Epub Date: 2026-02-02DOI: 10.1016/j.pedobi.2026.151115
Bárbara de Albuquerque Pereira , Cristiano Ilha , Antônio Domingos Brescovit , Luiz Felipe Moretti Iniesta , Rodrigo Salvador Bouzan , Luís Carlos Iuñes de Oliveira Filho , Dilmar Baretta , Amazonas Chagas Junior , Renan de Souza Rezende , Carolina Riviera Duarte Maluche Baretta
Landscape complexity, agricultural intensification and environmental gradients can affect the diversity and composition of diplopods and chilopods, important soil bioindicators. The objective was to evaluate the response of taxonomic and functional diversity, as well as the functional attributes of myriapod communities in land use systems. The study was carried out in the subtropical region of western Santa Catarina, covering the following land use systems: native forest (NF), eucalyptus reforestation (ER), pasture (PA), integrated crop-livestock farming (CLI) and no-tillage (NT). Sampling was carried out at 29 sites, approximately 167 m apart, totaling 87 sites. Two methods were used: Tropical Soil Biology and Fertility (TSBF) and soil traps. Functional diversity was characterized according to six functional traits: locomotion, feeding strategy, stratum preference, habitat affinity, moisture preference and sensitivity to disturbance. Taxonomic diversity was analyzed using Shannon and Simpson's richness and diversity. The FRic, FEve, FDis, FDiv and CWM indices were calculated. The highest taxonomic and functional richness of myriapods was found in NF and NT and the lowest in PA. The indicator species found were Lamyctes sp.1, Schendylidae sp. and Strongylomorpha araucariae. The environmental gradients: soil cover, OM, soil moisture, and the Ca, Mg, K and Al content of the soil were determining factors in the occurrence and functional composition of myriapods. The functional traits stratum preference, feeding strategy, habitat affinity and moisture preference were affected by land use changes. Taxonomic and functional diversity across environmental gradients and land use intensity are important bioindication tools for a group that has been little explored in South America.
{"title":"Patterns of taxonomic and functional diversity of Diplopoda and Chilopoda in land use systems","authors":"Bárbara de Albuquerque Pereira , Cristiano Ilha , Antônio Domingos Brescovit , Luiz Felipe Moretti Iniesta , Rodrigo Salvador Bouzan , Luís Carlos Iuñes de Oliveira Filho , Dilmar Baretta , Amazonas Chagas Junior , Renan de Souza Rezende , Carolina Riviera Duarte Maluche Baretta","doi":"10.1016/j.pedobi.2026.151115","DOIUrl":"10.1016/j.pedobi.2026.151115","url":null,"abstract":"<div><div>Landscape complexity, agricultural intensification and environmental gradients can affect the diversity and composition of diplopods and chilopods, important soil bioindicators. The objective was to evaluate the response of taxonomic and functional diversity, as well as the functional attributes of myriapod communities in land use systems. The study was carried out in the subtropical region of western Santa Catarina, covering the following land use systems: native forest (NF), eucalyptus reforestation (ER), pasture (PA), integrated crop-livestock farming (CLI) and no-tillage (NT). Sampling was carried out at 29 sites, approximately 167 m apart, totaling 87 sites. Two methods were used: Tropical Soil Biology and Fertility (TSBF) and soil traps. Functional diversity was characterized according to six functional traits: locomotion, feeding strategy, stratum preference, habitat affinity, moisture preference and sensitivity to disturbance. Taxonomic diversity was analyzed using Shannon and Simpson's richness and diversity. The FRic, FEve, FDis, FDiv and CWM indices were calculated. The highest taxonomic and functional richness of myriapods was found in NF and NT and the lowest in PA. The indicator species found were <em>Lamyctes</em> sp.1, <em>Schendylidae</em> sp. and <em>Strongylomorpha araucariae</em>. The environmental gradients: soil cover, OM, soil moisture, and the Ca, Mg, K and Al content of the soil were determining factors in the occurrence and functional composition of myriapods. The functional traits stratum preference, feeding strategy, habitat affinity and moisture preference were affected by land use changes. Taxonomic and functional diversity across environmental gradients and land use intensity are important bioindication tools for a group that has been little explored in South America.</div></div>","PeriodicalId":49711,"journal":{"name":"Pedobiologia","volume":"114 ","pages":"Article 151115"},"PeriodicalIF":2.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146173722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Corrigendum to: Asian knotweed’s impacts on soil chemistry and enzyme activities are higher in soils with low-nutrient status [vol. 107, November (2024), 151002]","authors":"Fanny Dommanget , Estelle Forey , Matthieu Chauvat , Amandine Erktan , Léa Daniès , Coralie Chesseron , Nicolas Fanin","doi":"10.1016/j.pedobi.2025.151097","DOIUrl":"10.1016/j.pedobi.2025.151097","url":null,"abstract":"","PeriodicalId":49711,"journal":{"name":"Pedobiologia","volume":"114 ","pages":"Article 151097"},"PeriodicalIF":2.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147396778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-01Epub Date: 2026-02-21DOI: 10.1016/j.pedobi.2026.151120
Xiaoyu Chen , Yiting Qiu , Zijia Hong , Shulei Wen , Chunming Xu , Feihua Zhou , Shengsheng Jin , Liuming Yang , Yong Zheng
Both arbuscular mycorrhizal (AM) and ectomycorrhizal (EM) trees co-exist in subtropical forest, and play key roles in regulating soil organic carbon (SOC). Here, based on a controlled experiment, the responses of microbial (fungal) biomass, particulate (POC) and mineral-associated organic carbon (MAOC) to an AM tree dominance (AMD) gradient and two soil depths were investigated in the wet and the dry seasons. The results showed that fungal biomass significantly decreased with increasing AMD regardless of soil depth and season. Although no significant differences in POC or MAOC across AMD levels were detected, the topsoil’s MAOC/SOC ratio varied significantly amongst AMD levels in the dry season, with the highest value detecting under 75% AMD treatment. As expected, higher fungal biomass, POC, MAOC, and SOC were observed in topsoil than in upper subsoil samples. POC was directly affected by soil fungal biomass, soil available phosphorus (AP) and total nitrogen (TN) in both seasons and influenced by AMD in the dry season, and also indirectly affected by AMD through fungal biomass regardless of season. However, MAOC was directly influenced by soil AP and TN in both seasons, and was concurrently affected by microbial biomass nitrogen in the dry season. Our findings suggest tentatively that soil fungal biomass responds more sensitively to the altered AMD compared with SOC fractions in subtropical young planted forests. This study improves the understanding of tree-fungal interactions triggering soil carbon sequestration potential.
{"title":"Effects of arbuscular mycorrhizal tree dominance and soil depth on microbial biomass and soil organic carbon fractions in a subtropical forest across the wet and dry seasons","authors":"Xiaoyu Chen , Yiting Qiu , Zijia Hong , Shulei Wen , Chunming Xu , Feihua Zhou , Shengsheng Jin , Liuming Yang , Yong Zheng","doi":"10.1016/j.pedobi.2026.151120","DOIUrl":"10.1016/j.pedobi.2026.151120","url":null,"abstract":"<div><div>Both arbuscular mycorrhizal (AM) and ectomycorrhizal (EM) trees co-exist in subtropical forest, and play key roles in regulating soil organic carbon (SOC). Here, based on a controlled experiment, the responses of microbial (fungal) biomass, particulate (POC) and mineral-associated organic carbon (MAOC) to an AM tree dominance (AMD) gradient and two soil depths were investigated in the wet and the dry seasons. The results showed that fungal biomass significantly decreased with increasing AMD regardless of soil depth and season. Although no significant differences in POC or MAOC across AMD levels were detected, the topsoil’s MAOC/SOC ratio varied significantly amongst AMD levels in the dry season, with the highest value detecting under 75% AMD treatment. As expected, higher fungal biomass, POC, MAOC, and SOC were observed in topsoil than in upper subsoil samples. POC was directly affected by soil fungal biomass, soil available phosphorus (AP) and total nitrogen (TN) in both seasons and influenced by AMD in the dry season, and also indirectly affected by AMD through fungal biomass regardless of season. However, MAOC was directly influenced by soil AP and TN in both seasons, and was concurrently affected by microbial biomass nitrogen in the dry season. Our findings suggest tentatively that soil fungal biomass responds more sensitively to the altered AMD compared with SOC fractions in subtropical young planted forests. This study improves the understanding of tree-fungal interactions triggering soil carbon sequestration potential.</div></div>","PeriodicalId":49711,"journal":{"name":"Pedobiologia","volume":"114 ","pages":"Article 151120"},"PeriodicalIF":2.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147396779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-01Epub Date: 2026-01-30DOI: 10.1016/j.pedobi.2026.151114
Shuqi Shen , Jueling Liu , Yongmeng Jiang , Yuming Lu , Cui Deng , Man Wang , Jinsheng Xie
Reforestation with broadleaf tree species in degraded Pinus massoniana plantations is a widely adopted strategy to enhance soil organic carbon (SOC) sequestration in subtropical China. However, the specific microbial processes that mediate this effect, particularly regarding nutrient cycling and microbial physiological efficiency, remain poorly understood. Here, we compared single pine stands with stands where broadleaf species were introduced, across a restoration chronosequence (10, 20, and 41 years). We aimed to elucidate how tree species mixing influences SOC storage through its effects on soil properties, microbial communities (with a focus on arbuscular mycorrhizal fungi, AMF), extracellular enzyme activities, and microbial carbon use efficiency (CUE). We found that broadleaf introduction increased SOC, total nitrogen, and AMF biomass, while stimulating the activity of nitrogen-acquiring enzymes (leucine aminopeptidase). In contrast, mixed stands showed reduced activity of phosphorus-acquiring enzymes, indicating an alleviation of soil phosphorus (P) limitation. Changes in microbial carbon metabolism accompanied these shifts, reflected in the altered CUE patterns. Structural equation modeling confirmed that the introduction of broadleaf promoted SOC accumulation primarily by increasing AMF abundance and alleviating P limitation, which collectively reshaped microbial community structure and function. This multi-pathway process enhanced the transformation of organic matter and microbial residue formation, contributing to greater SOC stabilization. Our results demonstrate that rehabilitating degraded pine forests with broadleaf species enhances carbon sequestration by modifying microbial nutrient limitation and metabolic strategies. Microbial CUE in this context reflects microbial metabolic status rather than functioning as a direct driver of SOC accumulation, providing a critical mechanistic basis for targeted forest management in subtropical region.
{"title":"Introducing tree species of Schima superba and Liquidambar formosana enhances soil carbon sequestration by alleviating microbial phosphorus limitation and modulating microbial metabolism in degraded pine forests","authors":"Shuqi Shen , Jueling Liu , Yongmeng Jiang , Yuming Lu , Cui Deng , Man Wang , Jinsheng Xie","doi":"10.1016/j.pedobi.2026.151114","DOIUrl":"10.1016/j.pedobi.2026.151114","url":null,"abstract":"<div><div>Reforestation with broadleaf tree species in degraded <em>Pinus massoniana</em> plantations is a widely adopted strategy to enhance soil organic carbon (SOC) sequestration in subtropical China. However, the specific microbial processes that mediate this effect, particularly regarding nutrient cycling and microbial physiological efficiency, remain poorly understood. Here, we compared single pine stands with stands where broadleaf species were introduced, across a restoration chronosequence (10, 20, and 41 years). We aimed to elucidate how tree species mixing influences SOC storage through its effects on soil properties, microbial communities (with a focus on arbuscular mycorrhizal fungi, AMF), extracellular enzyme activities, and microbial carbon use efficiency (CUE). We found that broadleaf introduction increased SOC, total nitrogen, and AMF biomass, while stimulating the activity of nitrogen-acquiring enzymes (leucine aminopeptidase). In contrast, mixed stands showed reduced activity of phosphorus-acquiring enzymes, indicating an alleviation of soil phosphorus (P) limitation. Changes in microbial carbon metabolism accompanied these shifts, reflected in the altered CUE patterns. Structural equation modeling confirmed that the introduction of broadleaf promoted SOC accumulation primarily by increasing AMF abundance and alleviating P limitation, which collectively reshaped microbial community structure and function. This multi-pathway process enhanced the transformation of organic matter and microbial residue formation, contributing to greater SOC stabilization. Our results demonstrate that rehabilitating degraded pine forests with broadleaf species enhances carbon sequestration by modifying microbial nutrient limitation and metabolic strategies. Microbial CUE in this context reflects microbial metabolic status rather than functioning as a direct driver of SOC accumulation, providing a critical mechanistic basis for targeted forest management in subtropical region.</div></div>","PeriodicalId":49711,"journal":{"name":"Pedobiologia","volume":"114 ","pages":"Article 151114"},"PeriodicalIF":2.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146078127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-01Epub Date: 2025-10-16DOI: 10.1016/j.pedobi.2025.151094
Yanbo An , Xiaotong Ji , Jiahui Zhang , Qifeng Wu , Wenhao Jin , Jiajia Xing , Hua Qin
Trichoderma guizhouense is recognized as an effective antagonistic fungus against Ralstonia solanacearum, the causative agent of tomato bacterial wilt. However, how T. guizhouense-enriched organic fertilizer affects the survival of R. solanacearum and bacterial community in tomato rhizosphere soil is still poorly understood. To address this, a pot experiment was conducted to assess changes in the abundance of the fliC gene (a marker for R. solanacearum) and to investigate shifts in the rhizosphere bacterial community after T. guizhouense-enriched organic fertilizer application using quantitative PCR and high-throughput sequencing, respectively. The results showed that T. guizhouense-enriched organic fertilizer application significantly reduced the abundance of fliC gene in tomato rhizosphere soil after 15 days of R. solanacearum inoculation, and notably decreased the disease index of tomato wilt after 13 days of R. solanacearum inoculation (P < 0.05). Compared to common organic fertilizer application alone, T. guizhouense-enriched organic fertilizer application significantly increased the Shannon index of soil bacterial community and enhanced the relative abundance of beneficial bacterial genera, including Caulobacter, Chitinophaga and Bacillus. Both T. guizhouense-enriched organic fertilizer application and R. solanacearum inoculation significantly altered soil bacterial community composition. Moreover, the bacterial co-occurrence network exhibited greater complexity and cohesiveness in the T. guizhouense-enriched organic fertilizer application treatment, which could contribute to maintaining the stability of rhizospheric bacterial community of R. solanacearum-infected tomato. The findings suggested that T. guizhouense-enriched organic fertilizer would significantly suppress bacterial wilt and changed the bacterial community structure of the soil.
{"title":"Trichoderma guizhouense enhances tomato (Solanum lycopersicum) Ralstonia wilt suppression by organic fertilizer, focusing on rhizosphere soil bacterial community","authors":"Yanbo An , Xiaotong Ji , Jiahui Zhang , Qifeng Wu , Wenhao Jin , Jiajia Xing , Hua Qin","doi":"10.1016/j.pedobi.2025.151094","DOIUrl":"10.1016/j.pedobi.2025.151094","url":null,"abstract":"<div><div><em>Trichoderma guizhouense</em> is recognized as an effective antagonistic fungus against <em>Ralstonia solanacearum</em>, the causative agent of tomato bacterial wilt<em>.</em> However, how <em>T. guizhouense</em>-enriched organic fertilizer affects the survival of <em>R. solanacearum</em> and bacterial community in tomato rhizosphere soil is still poorly understood. To address this, a pot experiment was conducted to assess changes in the abundance of the <em>fliC</em> gene (a marker for <em>R. solanacearum</em>) and to investigate shifts in the rhizosphere bacterial community after <em>T. guizhouense-</em>enriched organic fertilizer application using quantitative PCR and high-throughput sequencing, respectively. The results showed that <em>T. guizhouense-</em>enriched organic fertilizer application significantly reduced the abundance of <em>fliC</em> gene in tomato rhizosphere soil after 15 days of <em>R. solanacearum</em> inoculation, and notably decreased the disease index of tomato wilt after 13 days of <em>R. solanacearum</em> inoculation (<em>P</em> < 0.05). Compared to common organic fertilizer application alone, <em>T. guizhouense-</em>enriched organic fertilizer application significantly increased the Shannon index of soil bacterial community and enhanced the relative abundance of beneficial bacterial genera, including <em>Caulobacter, Chitinophaga</em> and <em>Bacillus</em>. Both <em>T. guizhouense-</em>enriched organic fertilizer application and <em>R. solanacearum</em> inoculation significantly altered soil bacterial community composition. Moreover, the bacterial co-occurrence network exhibited greater complexity and cohesiveness in the <em>T. guizhouense-</em>enriched organic fertilizer application treatment, which could contribute to maintaining the stability of rhizospheric bacterial community of <em>R. solanacearum</em>-infected tomato. The findings suggested that <em>T. guizhouense</em>-enriched organic fertilizer would significantly suppress bacterial wilt and changed the bacterial community structure of the soil.</div></div>","PeriodicalId":49711,"journal":{"name":"Pedobiologia","volume":"113 ","pages":"Article 151094"},"PeriodicalIF":2.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145325599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-01Epub Date: 2025-09-30DOI: 10.1016/j.pedobi.2025.151091
Dave Coleman, Zoë Lindo
{"title":"Putting the “Ped” into Pedobiologia –– A call to a new generation of soil ecologists","authors":"Dave Coleman, Zoë Lindo","doi":"10.1016/j.pedobi.2025.151091","DOIUrl":"10.1016/j.pedobi.2025.151091","url":null,"abstract":"","PeriodicalId":49711,"journal":{"name":"Pedobiologia","volume":"113 ","pages":"Article 151091"},"PeriodicalIF":2.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145736185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-01Epub Date: 2025-11-10DOI: 10.1016/j.pedobi.2025.151095
Dennis Castillo-Figueroa , Camilo Castillo-Avila , Miguel Angel Jaramillo
Centipedes are among the top predators regulating soil food webs in terrestrial ecosystems. Yet, they remain one of the least studied groups of soil fauna, particularly regarding their ecology and natural history. This gap is especially pronounced in upper Andean tropical forests (UATF), where few surveys have been conducted despite extensive habitat transformation. In this study, we investigated changes in centipede communities along a successional gradient in UATF and identified the ecological factors associated with changes in their abundance. We collected 168 soil samples across four UATF sites along a successional gradient in Colombia, covering all climatic seasons in 2022. Differences in species composition between sites were analyzed using ANOSIM, NMDS, and SIMPER. We also measured forest structure (litter depth, slope, canopy openness, leaf area index), microclimatic conditions (temperature, moisture), and prey availability (abundance, richness) to examine their relationships with centipede abundance through simple and multiple regressions. A total of 291 individuals from eight species were recorded, with each forest site showing a distinct community. Centipede abundance followed a hump-shaped pattern along succession, with one species indicative of secondary forests and another characteristic of mature forests. Temperature fluctuation and prey availability were the main factors associated with total centipede abundance. However, the two centipede orders responded differently: Scolopendromorpha were primarily associated with maximum temperature, while Geophilomorpha were linked to a combination of prey availability, temperature fluctuation, and slope. Our findings provide novel insights into centipede ecology in tropical montane forests, highlighting the role of environmental gradients in shaping predator communities.
{"title":"Unveiling the hidden soil predators of the Andes: Contrasting centipede responses to ecological factors along a successional gradient in tropical montane forests","authors":"Dennis Castillo-Figueroa , Camilo Castillo-Avila , Miguel Angel Jaramillo","doi":"10.1016/j.pedobi.2025.151095","DOIUrl":"10.1016/j.pedobi.2025.151095","url":null,"abstract":"<div><div>Centipedes are among the top predators regulating soil food webs in terrestrial ecosystems. Yet, they remain one of the least studied groups of soil fauna, particularly regarding their ecology and natural history. This gap is especially pronounced in upper Andean tropical forests (UATF), where few surveys have been conducted despite extensive habitat transformation. In this study, we investigated changes in centipede communities along a successional gradient in UATF and identified the ecological factors associated with changes in their abundance. We collected 168 soil samples across four UATF sites along a successional gradient in Colombia, covering all climatic seasons in 2022. Differences in species composition between sites were analyzed using ANOSIM, NMDS, and SIMPER. We also measured forest structure (litter depth, slope, canopy openness, leaf area index), microclimatic conditions (temperature, moisture), and prey availability (abundance, richness) to examine their relationships with centipede abundance through simple and multiple regressions. A total of 291 individuals from eight species were recorded, with each forest site showing a distinct community. Centipede abundance followed a hump-shaped pattern along succession, with one species indicative of secondary forests and another characteristic of mature forests. Temperature fluctuation and prey availability were the main factors associated with total centipede abundance. However, the two centipede orders responded differently: Scolopendromorpha were primarily associated with maximum temperature, while Geophilomorpha were linked to a combination of prey availability, temperature fluctuation, and slope. Our findings provide novel insights into centipede ecology in tropical montane forests, highlighting the role of environmental gradients in shaping predator communities.</div></div>","PeriodicalId":49711,"journal":{"name":"Pedobiologia","volume":"113 ","pages":"Article 151095"},"PeriodicalIF":2.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145578722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-01Epub Date: 2025-11-23DOI: 10.1016/j.pedobi.2025.151099
Shijia Wang, Xia Peng, Jian Su, Lihua Tu
A clear understanding of microbial necromass carbon (MRC) accumulation mechanisms is essential for evaluating stable carbon budgets and informing effective strategies to enhance permanent carbon storage on a global forest scale. However, the complexity and heterogeneity inherent in forest systems have left the distribution of MRC at finer forest-type classification scales, and its driving factors still need to be solved. Thus, this study synthesized a dataset of 1220 data points from 88 peer-reviewed papers on global forests to investigate the distribution and mechanisms of MRC across diverse climate zones, forest types, elevation gradients, and soil depths. The analysis showed that temperate regions, with lower mean annual temperature and precipitation, had the highest MRC contents (21.88 g kg⁻¹), especially in mixed forests. MRC accumulation was further favored by low- to mid-elevations, Alfisols, and surface soil layers. Climate factors exerted both direct positive effects on MRC and indirect influences through microbial community structure and soil substrate properties. Overall, this global synthesis enhances our understanding of the environmental mechanisms governing MRC and its contribution to stable soil carbon storage in forests.
明确微生物坏死体碳（MRC）积累机制对于评估稳定的碳预算和提供有效的策略以提高全球森林规模的永久碳储量至关重要。然而，森林系统固有的复杂性和异质性使得MRC的分布在更精细的森林类型分类尺度上，其驱动因素仍有待解决。因此，本研究综合了来自88篇全球森林同行评议论文的1220个数据点数据集，探讨了不同气候带、森林类型、高程梯度和土壤深度的MRC分布及其机制。分析表明，年平均气温和降水量较低的温带地区，尤其是混交林，其MRC含量最高（21.88 g kg⁻¹）。中低海拔、土层和表层更有利于MRC的积累。气候因子通过微生物群落结构和土壤基质特性对土壤MRC既有直接的正向影响，也有间接的影响。总的来说，这一全球综合增强了我们对控制MRC的环境机制及其对森林土壤碳稳定储存的贡献的理解。
{"title":"Global synthesis reveals that climate and soil substrate shape microbial necromass carbon in forest soils","authors":"Shijia Wang, Xia Peng, Jian Su, Lihua Tu","doi":"10.1016/j.pedobi.2025.151099","DOIUrl":"10.1016/j.pedobi.2025.151099","url":null,"abstract":"<div><div>A clear understanding of microbial necromass carbon (MRC) accumulation mechanisms is essential for evaluating stable carbon budgets and informing effective strategies to enhance permanent carbon storage on a global forest scale. However, the complexity and heterogeneity inherent in forest systems have left the distribution of MRC at finer forest-type classification scales, and its driving factors still need to be solved. Thus, this study synthesized a dataset of 1220 data points from 88 peer-reviewed papers on global forests to investigate the distribution and mechanisms of MRC across diverse climate zones, forest types, elevation gradients, and soil depths. The analysis showed that temperate regions, with lower mean annual temperature and precipitation, had the highest MRC contents (21.88 g kg⁻¹), especially in mixed forests. MRC accumulation was further favored by low- to mid-elevations, Alfisols, and surface soil layers. Climate factors exerted both direct positive effects on MRC and indirect influences through microbial community structure and soil substrate properties. Overall, this global synthesis enhances our understanding of the environmental mechanisms governing MRC and its contribution to stable soil carbon storage in forests.</div></div>","PeriodicalId":49711,"journal":{"name":"Pedobiologia","volume":"113 ","pages":"Article 151099"},"PeriodicalIF":2.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145623735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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