Pub Date : 2024-12-09DOI: 10.1016/j.legalmed.2024.102559
Tadashi Hosoya, Kazuki Harada, Jun Kanetake
Using human cardiac blood from forensic autopsy cases, comprehensive measurements of albumin, vitamins A, B1, B6, B12, C, D, folate, and PIVKA-Ⅱ were performed. Of 128 cases, 83 were male, with an average age of 61.8 years and average postmortem interval of 44 h. The average concentrations of vitamins were 29.8 μg/dL for vitamin A, 32.2 μg/dL for vitamin B1, 278 ng/mL for vitamin B6 (pyridoxamine), 152 ng/mL for vitamin B6 (pyridoxal), 57.2 ng/mL for vitamin B6 (pyridoxal), 17.9 ng/mL for folate, 1188 pg/mL for vitamin B12, 23.5 μg/mL for vitamin C, 15.0 ng/mL for vitamin D, and 1.03 μg/mL for PIVKA-Ⅱ. An autopsy case of a Japanese middle-aged male with beriberi was also presented, where severe edema and effusion of the cavity and a high NT-proBNP serum value were observed; however, cardiac pathology showed no specific abnormal features. In the present case, the blood vitamin B1 concentration was within the standard clinical range. This study revealed that bloodborne water-soluble vitamin levels shows higher to clinical standard value at postmortem, whereas fat-soluble vitamin levels may stay in the standard range or lower. Our findings suggest that postmortem water-soluble vitamin concentrations within the clinical standard may reflect low antemortem vitamin concentrations.
{"title":"Comprehensive study of various vitamin concentrations in the human postmortem blood with an autopsy case report of beriberi.","authors":"Tadashi Hosoya, Kazuki Harada, Jun Kanetake","doi":"10.1016/j.legalmed.2024.102559","DOIUrl":"https://doi.org/10.1016/j.legalmed.2024.102559","url":null,"abstract":"<p><p>Using human cardiac blood from forensic autopsy cases, comprehensive measurements of albumin, vitamins A, B1, B6, B12, C, D, folate, and PIVKA-Ⅱ were performed. Of 128 cases, 83 were male, with an average age of 61.8 years and average postmortem interval of 44 h. The average concentrations of vitamins were 29.8 μg/dL for vitamin A, 32.2 μg/dL for vitamin B1, 278 ng/mL for vitamin B6 (pyridoxamine), 152 ng/mL for vitamin B6 (pyridoxal), 57.2 ng/mL for vitamin B6 (pyridoxal), 17.9 ng/mL for folate, 1188 pg/mL for vitamin B12, 23.5 μg/mL for vitamin C, 15.0 ng/mL for vitamin D, and 1.03 μg/mL for PIVKA-Ⅱ. An autopsy case of a Japanese middle-aged male with beriberi was also presented, where severe edema and effusion of the cavity and a high NT-proBNP serum value were observed; however, cardiac pathology showed no specific abnormal features. In the present case, the blood vitamin B1 concentration was within the standard clinical range. This study revealed that bloodborne water-soluble vitamin levels shows higher to clinical standard value at postmortem, whereas fat-soluble vitamin levels may stay in the standard range or lower. Our findings suggest that postmortem water-soluble vitamin concentrations within the clinical standard may reflect low antemortem vitamin concentrations.</p>","PeriodicalId":49913,"journal":{"name":"Legal Medicine","volume":"72 ","pages":"102559"},"PeriodicalIF":1.3,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142822649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-07DOI: 10.1016/j.legalmed.2024.102558
Shuai Luo, Fei Fan, Meng Liu, Li-Rong Qiu, Meng-Jun Zhan, Yu-Chi Zhou, Hui-Kun Yang, Hu Chen, Xue-Ling Chen, Zhen-Hua Deng
This case presents a unique age estimation involving Chinese Tibetan twins, where one of them has been arrested for alleged rape. His parents claimed he was about 13 years old without providing any official age documentation. Since age is important in criminal proceedings in China, bone age estimation was conducted to determine the chronological age of the twins. The twins underwent a physical examination, X-rays of the left hand/wrist, and pelvis, and a CT scan of the clavicle. Two age estimation standards in China were used: "Skeletal Maturity and Assessment Methods of Hand and Wrist for Chinese" (China-05 standard), and "Technical Specifications for Skeletal Age Evaluation of Han Population Teenagers" (TSSHPT standard). The bone radiographs showed that most of the twins' epiphyseal development was similar, with only slight differences observed in the distal radius, distal ulna, and iliac crest apophysis. The results of the two methods showed differences in the range of predicted ages. Applying the RUS-CHN method to hand/wrist images, the estimated age of the suspect was determined to be 16-16.5 years old, while his brother's age was 15.5-16 years old. Applying the TSSHPT standard to all three images, the estimated age of the suspect was 17-17.5 years old, while his brother's age was estimated to be 16.5-17 years old. In this case, two commonly used methods for age estimation were introduced. The disparity in age estimations using the RUS-CHN and TSSHPT standards, with the age of the suspect ranging from 16 to 17.5 years. This case revealed that the choice of bone age estimation method can significantly impact the result of estimated age, which has important implications for legal proceedings. Additionally, the minor differences in skeletal maturity between the twins emphasize the need to account for individual variations, even among genetically similar individuals. Furthermore, this case contributes to the broader discourse on the effectiveness of skeletal age estimation methods across diverse populations, underscoring the necessity for tailored evaluation standards that consider genetic and environmental diversity.
{"title":"Age assessment of Chinese Tibetan twins using multiple skeletal age estimation methods: A case report.","authors":"Shuai Luo, Fei Fan, Meng Liu, Li-Rong Qiu, Meng-Jun Zhan, Yu-Chi Zhou, Hui-Kun Yang, Hu Chen, Xue-Ling Chen, Zhen-Hua Deng","doi":"10.1016/j.legalmed.2024.102558","DOIUrl":"https://doi.org/10.1016/j.legalmed.2024.102558","url":null,"abstract":"<p><p>This case presents a unique age estimation involving Chinese Tibetan twins, where one of them has been arrested for alleged rape. His parents claimed he was about 13 years old without providing any official age documentation. Since age is important in criminal proceedings in China, bone age estimation was conducted to determine the chronological age of the twins. The twins underwent a physical examination, X-rays of the left hand/wrist, and pelvis, and a CT scan of the clavicle. Two age estimation standards in China were used: \"Skeletal Maturity and Assessment Methods of Hand and Wrist for Chinese\" (China-05 standard), and \"Technical Specifications for Skeletal Age Evaluation of Han Population Teenagers\" (TSSHPT standard). The bone radiographs showed that most of the twins' epiphyseal development was similar, with only slight differences observed in the distal radius, distal ulna, and iliac crest apophysis. The results of the two methods showed differences in the range of predicted ages. Applying the RUS-CHN method to hand/wrist images, the estimated age of the suspect was determined to be 16-16.5 years old, while his brother's age was 15.5-16 years old. Applying the TSSHPT standard to all three images, the estimated age of the suspect was 17-17.5 years old, while his brother's age was estimated to be 16.5-17 years old. In this case, two commonly used methods for age estimation were introduced. The disparity in age estimations using the RUS-CHN and TSSHPT standards, with the age of the suspect ranging from 16 to 17.5 years. This case revealed that the choice of bone age estimation method can significantly impact the result of estimated age, which has important implications for legal proceedings. Additionally, the minor differences in skeletal maturity between the twins emphasize the need to account for individual variations, even among genetically similar individuals. Furthermore, this case contributes to the broader discourse on the effectiveness of skeletal age estimation methods across diverse populations, underscoring the necessity for tailored evaluation standards that consider genetic and environmental diversity.</p>","PeriodicalId":49913,"journal":{"name":"Legal Medicine","volume":"72 ","pages":"102558"},"PeriodicalIF":1.3,"publicationDate":"2024-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142830692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-01DOI: 10.1016/j.legalmed.2024.102557
Beáta Ágnes Borsay, Barbara Dóra Halasi, Róbert Kristóf Pórszász, Péter Attila Gergely
The focus is on unnatural death in forensic pathology including criminal death. One special field is the murder-suicide or homicide-suicide. It is a relatively rare occurrence worldwide mainly with male perpetrators and female victims. The authors report a case about a special type of homicide-suicide which is so-called intimate partner homicide (femicide)-suicide in which the victim was a 42-year-old woman with more than 55 incised- (cuts, stabs, etc.) and blunt force injuries with external examination. The resuscitation was unsuccessful by the paramedics. Not one of the wounds was incompatible with life, some of them had life-threatening conditions, and numerous self-defense type of wounds were observed. Not so far from the crime scene, the 48-year-old perpetrator's hanging corpse was found on a high voltage electric column who previously committed suicide, talked to one of his daughters from his first marriage, left money at his funeral, and asserted he would not have gone to prison again. The authors compared this case with the international literature data.
{"title":"Homicide-suicide as domestic violence: A case report with a little literature review.","authors":"Beáta Ágnes Borsay, Barbara Dóra Halasi, Róbert Kristóf Pórszász, Péter Attila Gergely","doi":"10.1016/j.legalmed.2024.102557","DOIUrl":"https://doi.org/10.1016/j.legalmed.2024.102557","url":null,"abstract":"<p><p>The focus is on unnatural death in forensic pathology including criminal death. One special field is the murder-suicide or homicide-suicide. It is a relatively rare occurrence worldwide mainly with male perpetrators and female victims. The authors report a case about a special type of homicide-suicide which is so-called intimate partner homicide (femicide)-suicide in which the victim was a 42-year-old woman with more than 55 incised- (cuts, stabs, etc.) and blunt force injuries with external examination. The resuscitation was unsuccessful by the paramedics. Not one of the wounds was incompatible with life, some of them had life-threatening conditions, and numerous self-defense type of wounds were observed. Not so far from the crime scene, the 48-year-old perpetrator's hanging corpse was found on a high voltage electric column who previously committed suicide, talked to one of his daughters from his first marriage, left money at his funeral, and asserted he would not have gone to prison again. The authors compared this case with the international literature data.</p>","PeriodicalId":49913,"journal":{"name":"Legal Medicine","volume":"72 ","pages":"102557"},"PeriodicalIF":1.3,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142787490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A common forensic problem is cervical fractures, which sometimes need to be diagnosed only by physical examination of the body, without imaging or autopsy. Despite reports from clinical practice describing the association between cervical fractures and head injury, we could not find any analysis of autopsy cases. In addition, discussion of the cervical fracture diagnosis by postmortem computed tomography (PMCT) appears limited. This study aimed to examine autopsy and PMCT findings and explore valid methods for the diagnosis. We analyzed autopsy cases of cervical fractures during the 7 years before (2004-2010) and after (2014-2020) the PMCT introduction in our department. In 2014-2020, 67 autopsy cases with cervical fractures were recorded, of which 61 (91 %) were related to blunt injury to the head. Those with cervical fractures had a significantly higher incidence of blunt injury to the head (p < 0.001), particularly "forehead" and "face" injury, which accounted for > 50 % of cases. Of the external forces on the neck, "extension" accounted for 82.1 %. The cervical fracture-positive rate in all autopsy cases increased significantly from 11 (4.0 %) in 2004-2010 to 67 (8.2 %) in 2014-2020 (p = 0.021). From December 2015 to December 2020, when "cervical retroflexion" imaging was actively performed in PMCT, the cervical fracture diagnosis rate increased significantly from 57.1 % in the cervical normal position to 81.0 % with the addition of "cervical retroflexion" position (p = 0.021). Blunt head injury and PMCT in the "cervical retroflexion" position may be useful in the diagnosis of cervical fractures.
{"title":"Effective diagnosis of cervical fracture using postmortem computed tomography and autopsy findings.","authors":"Yoriko Shinba, Yuki Abe, Takanori Kohyama, Masahide Mitsuma, Hiromi Yamashita, Takehiko Murase, Kazuya Ikematsu","doi":"10.1016/j.legalmed.2024.102555","DOIUrl":"https://doi.org/10.1016/j.legalmed.2024.102555","url":null,"abstract":"<p><p>A common forensic problem is cervical fractures, which sometimes need to be diagnosed only by physical examination of the body, without imaging or autopsy. Despite reports from clinical practice describing the association between cervical fractures and head injury, we could not find any analysis of autopsy cases. In addition, discussion of the cervical fracture diagnosis by postmortem computed tomography (PMCT) appears limited. This study aimed to examine autopsy and PMCT findings and explore valid methods for the diagnosis. We analyzed autopsy cases of cervical fractures during the 7 years before (2004-2010) and after (2014-2020) the PMCT introduction in our department. In 2014-2020, 67 autopsy cases with cervical fractures were recorded, of which 61 (91 %) were related to blunt injury to the head. Those with cervical fractures had a significantly higher incidence of blunt injury to the head (p < 0.001), particularly \"forehead\" and \"face\" injury, which accounted for > 50 % of cases. Of the external forces on the neck, \"extension\" accounted for 82.1 %. The cervical fracture-positive rate in all autopsy cases increased significantly from 11 (4.0 %) in 2004-2010 to 67 (8.2 %) in 2014-2020 (p = 0.021). From December 2015 to December 2020, when \"cervical retroflexion\" imaging was actively performed in PMCT, the cervical fracture diagnosis rate increased significantly from 57.1 % in the cervical normal position to 81.0 % with the addition of \"cervical retroflexion\" position (p = 0.021). Blunt head injury and PMCT in the \"cervical retroflexion\" position may be useful in the diagnosis of cervical fractures.</p>","PeriodicalId":49913,"journal":{"name":"Legal Medicine","volume":"72 ","pages":"102555"},"PeriodicalIF":1.3,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142824566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Human-bear conflicts are a growing concern globally, with Russia harboring a significant population of brown bears. This case report details a fatal encounter between a male hunter and a brown bear in the greater Khabarovsk region. On October 2015, the hunter’s body was discovered approximately 400 m from a deceased brown bear, indicating a deadly confrontation. Forensic examination revealed extensive trauma and varied patterns of injuries, with the cause of death attributed to mixed shock from traumatic injuries and acute blood loss. Despite the limited dental information due to the missing maxilla, positive identification was achieved through the unique dental patterns in the victim’s mandible. This study emphasizes the need for specialized forensic knowledge in wildlife-related fatalities, highlighting the importance of accurate post-mortem examinations and the critical role of dental data in positively identifying victims under challenging circumstances.
{"title":"Fatal bear attack in Russian Federation: A case report","authors":"Nikolaos Angelakopoulos , Igor Valentinovich Vlasyuk , Sudheer Babu Balla , Rizky Merdietio Boedi , Galina Zolotenkova","doi":"10.1016/j.legalmed.2024.102556","DOIUrl":"10.1016/j.legalmed.2024.102556","url":null,"abstract":"<div><div>Human-bear conflicts are a growing concern globally, with Russia harboring a significant population of brown bears. This case report details a fatal encounter between a male hunter and a brown bear in the greater Khabarovsk region. On October 2015, the hunter’s body was discovered approximately 400 m from a deceased brown bear, indicating a deadly confrontation. Forensic examination revealed extensive trauma and varied patterns of injuries, with the cause of death attributed to mixed shock from traumatic injuries and acute blood loss. Despite the limited dental information due to the missing maxilla, positive identification was achieved through the unique dental patterns in the victim’s mandible. This study emphasizes the need for specialized forensic knowledge in wildlife-related fatalities, highlighting the importance of accurate post-mortem examinations and the critical role of dental data in positively identifying victims under challenging circumstances.</div></div>","PeriodicalId":49913,"journal":{"name":"Legal Medicine","volume":"72 ","pages":"Article 102556"},"PeriodicalIF":1.3,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142719858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-09DOI: 10.1016/j.legalmed.2024.102545
Rehab A. Azouz , Alaa F. Bakr , Marwa A. Ibrahim , Mohamed Y. Mahmoud
The postmortem identification of drowning in the field of forensic medicine is difficult due to unspecific autopsy findings, and usually, it is a “diagnosis of exclusion”. A model of drowning in salt and fresh water was established to discuss the postmortem changes after drowning and the differences between saltwater drowning (SWD) and freshwater drowning (FWD). The organs (brain and ‘lung) of 30 rats were extracted at three-time points (0 h, 24 h, and 48 h) after drowning. The histopathological, immunohistochemical,l, and molecular changes in the lung and brain of rats at different time points were investigated. Results show no significant difference in pathological findings between fresh and saltwater drowning. Casp3, JNK, and ERK all showed a rise in their postmortem expression in a time-dependent way; the expression of these three genes is much greater in cases of saltwater drowning compared to cases of freshwater drowning. So, it is concluded that after 24 h and 48 h from death, potent cellular oxidative stress occurred and caused the upregulation of the studied genes.
{"title":"Immunohistochemical and molecular study for differential diagnosis between freshwater and saltwater drowning","authors":"Rehab A. Azouz , Alaa F. Bakr , Marwa A. Ibrahim , Mohamed Y. Mahmoud","doi":"10.1016/j.legalmed.2024.102545","DOIUrl":"10.1016/j.legalmed.2024.102545","url":null,"abstract":"<div><div>The postmortem identification of drowning in the field of forensic medicine is difficult due to unspecific autopsy findings, and usually, it is a “diagnosis of exclusion”. A model of drowning in salt and fresh water was established to discuss the postmortem changes after drowning and the differences between saltwater drowning (SWD) and freshwater drowning (FWD). The organs (brain and ‘lung) of 30 rats were extracted at three-time points (0 h, 24 h, and 48 h) after drowning. The histopathological, immunohistochemical,l, and molecular changes in the lung and brain of rats at different time points were investigated. Results show no significant difference in pathological findings between fresh and saltwater drowning. Casp3, JNK, and ERK all showed a rise in their postmortem expression in a time-dependent way; the expression of these three genes is much greater in cases of saltwater drowning compared to cases of freshwater drowning. So, it is concluded that after 24 h and 48 h from death, potent cellular oxidative stress occurred and caused the upregulation of the studied genes.</div></div>","PeriodicalId":49913,"journal":{"name":"Legal Medicine","volume":"72 ","pages":"Article 102545"},"PeriodicalIF":1.3,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142693860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The diatom test is one of the methods used to diagnose drowning in forensic autopsies. Metagenomic diatom analysis may reveal where a drowning occurred. We evaluated whether metagenomic diatom analysis could be used to infer waters, watersheds, and geographic locations using 166 water samples from 64 locations (freshwater: 55; seawater: 9). Principal component analysis (PCA) in all samples revealed no specific clusters for waters or watersheds. In one river, the three samples at the same site generally tended to be in close clusters, but there were some cases where the three sites were far from each other. The precise geographic location could thus not be reliably identified. However, PCA of data from dams, lakes, and retention basins revealed sites with independent clusters, suggesting unique diatom compositions. Diatoms of seawater were not detected in freshwater. The high number of Actinoptychus, Chaetoceros, and Skeletonema detected in seawater samples suggested that they are useful for seawater identification. This method required only 2 mL of water; it suggests that this method can be applied to actual samples. In summary, it was difficult to infer the geographic location and waters or watersheds, but the freshwater/seawater distinction could be easily made, and depending on the application, it may be useful in forensic science practice.
{"title":"The utility of drowning site inference through metagenomic diatom analysis","authors":"Hiroaki Nakanishi , Aya Takada , Katsumi Yoneyama , Saki Kodama , Kentaro Sakai , Kazuyuki Saito","doi":"10.1016/j.legalmed.2024.102548","DOIUrl":"10.1016/j.legalmed.2024.102548","url":null,"abstract":"<div><div>The diatom test is one of the methods used to diagnose drowning in forensic autopsies. Metagenomic diatom analysis may reveal where a drowning occurred. We evaluated whether metagenomic diatom analysis could be used to infer waters, watersheds, and geographic locations using 166 water samples from 64 locations (freshwater: 55; seawater: 9). Principal component analysis (PCA) in all samples revealed no specific clusters for waters or watersheds. In one river, the three samples at the same site generally tended to be in close clusters, but there were some cases where the three sites were far from each other. The precise geographic location could thus not be reliably identified. However, PCA of data from dams, lakes, and retention basins revealed sites with independent clusters, suggesting unique diatom compositions. Diatoms of seawater were not detected in freshwater. The high number of <em>Actinoptychus</em>, <em>Chaetoceros</em>, and <em>Skeletonema</em> detected in seawater samples suggested that they are useful for seawater identification. This method required only 2 mL of water; it suggests that this method can be applied to actual samples. In summary, it was difficult to infer the geographic location and waters or watersheds, but the freshwater/seawater distinction could be easily made, and depending on the application, it may be useful in forensic science practice.</div></div>","PeriodicalId":49913,"journal":{"name":"Legal Medicine","volume":"71 ","pages":"Article 102548"},"PeriodicalIF":1.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142640092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-01DOI: 10.1016/j.legalmed.2024.102532
Xiaoxin Hu , Jinjie Liu , Tingyu Xu , Kaiyue Qin , Yunpeng Feng , Zhenjun Jia , Xingchun Zhao
Third-generation sequencing technologies, exemplified by single-molecule real-time sequencing and nanopore sequencing, provide a constellation of advantages, including long read lengths, high throughput, real-time sequencing capabilities, and remarkable portability. These cutting-edge methodologies have provided new tools for genomic analysis in forensic medicine. To gain a comprehensive understanding of the current applications and cutting-edge trends of third-generation sequencing technologies in forensic medicine, this study retrieved relevant literature from the China National Knowledge Infrastructure (CNKI) database and the Web of Science (WOS) database. Using bibliometric software CiteSpace 6.1.R6, the study visualized publication volume, countries, and keywords related to the application of third-generation sequencing technologies in forensic medicine from 2014 to 2023. The review then summarized the foundational principles, characteristics, and promising prospects of third-generation sequencing technologies in forensic medicine. Notably, it highlights their remarkable contributions in forensic individual identification, body fluid identification, forensic epigenetic analysis, microbial analysis and forensic species identification.
{"title":"Research progress and application of the third-generation sequencing technologies in forensic medicine","authors":"Xiaoxin Hu , Jinjie Liu , Tingyu Xu , Kaiyue Qin , Yunpeng Feng , Zhenjun Jia , Xingchun Zhao","doi":"10.1016/j.legalmed.2024.102532","DOIUrl":"10.1016/j.legalmed.2024.102532","url":null,"abstract":"<div><div>Third-generation sequencing technologies, exemplified by single-molecule real-time sequencing and nanopore sequencing, provide a constellation of advantages, including long read lengths, high throughput, real-time sequencing capabilities, and remarkable portability. These cutting-edge methodologies have provided new tools for genomic analysis in forensic medicine. To gain a comprehensive understanding of the current applications and cutting-edge trends of third-generation sequencing technologies in forensic medicine, this study retrieved relevant literature from the China National Knowledge Infrastructure (CNKI) database and the Web of Science (WOS) database. Using bibliometric software CiteSpace 6.1.R6, the study visualized publication volume, countries, and keywords related to the application of third-generation sequencing technologies in forensic medicine from 2014 to 2023. The review then summarized the foundational principles, characteristics, and promising prospects of third-generation sequencing technologies in forensic medicine. Notably, it highlights their remarkable contributions in forensic individual identification, body fluid identification, forensic epigenetic analysis, microbial analysis and forensic species identification.</div></div>","PeriodicalId":49913,"journal":{"name":"Legal Medicine","volume":"71 ","pages":"Article 102532"},"PeriodicalIF":1.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142586609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-01DOI: 10.1016/j.legalmed.2024.102547
Alberto Amadasi, Larissa Amadasi
Wakeboarding is a sport associated with various types of injuries, primarily affecting the upper and lower limbs. In this case, a 44-year-old man fell from a ramp while wakeboarding and barely managed to reach the shore before dying shortly afterward. An autopsy revealed a complete rupture of the thoracic aorta along with a fracture of the fourth thoracic vertebra. It is likely that several mechanisms contributed to these injuries, either individually or in combination: concussive, rotational, and tensile forces. Additionally, it is plausible that the rupture occurred in two stages, with an initial partial injury worsening during subsequent movements or attempts at rescue and resuscitation. This is the first reported case of death resulting from a ruptured aorta due to wakeboarding, highlighting a potential consequence of this sport.
{"title":"Complete aortic rupture following wakeboarding accident","authors":"Alberto Amadasi, Larissa Amadasi","doi":"10.1016/j.legalmed.2024.102547","DOIUrl":"10.1016/j.legalmed.2024.102547","url":null,"abstract":"<div><div>Wakeboarding is a sport associated with various types of injuries, primarily affecting the upper and lower limbs. In this case, a 44-year-old man fell from a ramp while wakeboarding and barely managed to reach the shore before dying shortly afterward. An autopsy revealed a complete rupture of the thoracic aorta along with a fracture of the fourth thoracic vertebra. It is likely that several mechanisms contributed to these injuries, either individually or in combination: concussive, rotational, and tensile forces. Additionally, it is plausible that the rupture occurred in two stages, with an initial partial injury worsening during subsequent movements or attempts at rescue and resuscitation. This is the first reported case of death resulting from a ruptured aorta due to wakeboarding, highlighting a potential consequence of this sport.</div></div>","PeriodicalId":49913,"journal":{"name":"Legal Medicine","volume":"71 ","pages":"Article 102547"},"PeriodicalIF":1.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142564031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Age estimation has extensive medicolegal implications in civil and criminal identification. Despite the surge in adopting radiological investigations to assess developmental bony changes, the shoulder joint is understudied. A cross-sectional study was conducted, enrolling 283 shoulder radiographs of Egyptians, investigating the reliability of two previously established scores as predictors of chronological age using the epiphyseal maturation of proximal humerus and acromion process. Epiphyseal union of proximal humerus commenced at age of 16.1–17 and completed around 21, while complete acromial union was observed around the age of 20.8. Females significantly preceded males and showed lower mean total Scores A and B at different maturation stages. There was a significant strong positive correlation between the chronological age and the epiphyseal maturation of humerus, acromion and total shoulder scores with correlation coefficients between 0.84 and 0.9. The receiver operating characteristic curves showed significant discriminating power of the total shoulder Scores A and B as predictors of the ages of 14 and 16, with area under curves above 0.9, minimal accuracy of 96.5 % and p values of 0.001. Six proposed models were established where the model “age = 0.318 + (0.388) total shoulder Score A + (2.842) total shoulder Score B + 1.931 (sex)” showed the best significant prediction power of radiographic evaluation of epiphyseal maturation in the proximal humerus and acromion in estimating the ages between 8 and around 20 years (R2 of 0.812). Applying this model to assess the chronological age, especially if the results from the hand and teeth are inconclusive, is promising.
年龄估计在民事和刑事鉴定中具有广泛的医学法律意义。尽管采用放射学检查来评估骨骼发育变化的趋势日益明显,但对肩关节的研究却不足。这项横断面研究收集了 283 张埃及人的肩部 X 光片,利用肱骨近端和肩峰突的骨骺成熟情况,对之前确定的两个预测年龄的分数的可靠性进行了调查。肱骨近端骺端结合始于 16.1-17 岁,完成于 21 岁左右,而肩峰完全结合则是在 20.8 岁左右。女性明显早于男性,且在不同成熟阶段的平均总分 A 和 B 都较低。计时年龄与肱骨、肩峰的骨骺成熟度和肩关节总分之间存在明显的正相关,相关系数在 0.84 和 0.9 之间。接受者操作特征曲线显示,肩关节总评分 A 和 B 对 14 岁和 16 岁的预测具有显著的鉴别力,曲线下面积大于 0.9,最小准确率为 96.5 %,P 值为 0.001。我们建立了六个拟议模型,其中 "年龄 = 0.318 + (0.388) 肩部总分 A + (2.842) 肩部总分 B + 1.931(性别)"模型显示,肱骨近端和肩峰骺端骨骺成熟度的影像学评估对估计 8 岁至 20 岁左右的年龄具有最佳的显著预测能力(R2 为 0.812)。应用该模型评估法定年龄,尤其是在手部和牙齿的评估结果不确定的情况下,是很有前景的。
{"title":"Evaluation of two scoring systems assessing the epiphyseal union at shoulder joint as predictors of chronological age among a sample of Egyptians","authors":"Asmaa F. Sharif , Hadeel Eid , Mahmoud Abdelaziz Abdelnaby Ghalab , Asmaa Ali Ahmed Elfeky , Mohamed Moharram Badawy , Nagwa Mahmoud Habib , Reham Hassan El-Farouny , Heba A.A. Mabrouk","doi":"10.1016/j.legalmed.2024.102546","DOIUrl":"10.1016/j.legalmed.2024.102546","url":null,"abstract":"<div><div>Age estimation has extensive medicolegal implications in civil and criminal identification. Despite the surge in adopting radiological investigations to assess developmental bony changes, the shoulder joint is understudied. A cross-sectional study was conducted, enrolling 283 shoulder radiographs of Egyptians, investigating the reliability of two previously established scores as predictors of chronological age using the epiphyseal maturation of proximal humerus and acromion process. Epiphyseal union of proximal humerus commenced at age of 16.1–17 and completed around 21, while complete acromial union was observed around the age of 20.8. Females significantly preceded males and showed lower mean total Scores A and B at different maturation stages. There was a significant strong positive correlation between the chronological age and the epiphyseal maturation of humerus, acromion and total shoulder scores with correlation coefficients between 0.84 and 0.9. The receiver operating characteristic curves showed significant discriminating power of the total shoulder Scores A and B as predictors of the ages of 14 and 16, with area under curves above 0.9, minimal accuracy of 96.5 % and p values of 0.001. Six proposed models were established where the model <em>“age = 0.318 + (0.388) total shoulder Score A + (2.842) total shoulder Score B + 1.931 (sex)”</em> showed the best significant prediction power of radiographic evaluation of epiphyseal maturation in the proximal humerus and acromion in estimating the ages between 8 and around 20 years (R<sup>2</sup> of 0.812). Applying this model to assess the chronological age, especially if the results from the hand and teeth are inconclusive, is promising.</div></div>","PeriodicalId":49913,"journal":{"name":"Legal Medicine","volume":"71 ","pages":"Article 102546"},"PeriodicalIF":1.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142579098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}