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Comprehensive study of various vitamin concentrations in the human postmortem blood with an autopsy case report of beriberi. 人体死后血液中各种维生素浓度的综合研究,以及一例脚气病尸检报告。
IF 1.3 4区 医学 Q3 MEDICINE, LEGAL Pub Date : 2024-12-09 DOI: 10.1016/j.legalmed.2024.102559
Tadashi Hosoya, Kazuki Harada, Jun Kanetake

Using human cardiac blood from forensic autopsy cases, comprehensive measurements of albumin, vitamins A, B1, B6, B12, C, D, folate, and PIVKA-Ⅱ were performed. Of 128 cases, 83 were male, with an average age of 61.8 years and average postmortem interval of 44 h. The average concentrations of vitamins were 29.8 μg/dL for vitamin A, 32.2 μg/dL for vitamin B1, 278 ng/mL for vitamin B6 (pyridoxamine), 152 ng/mL for vitamin B6 (pyridoxal), 57.2 ng/mL for vitamin B6 (pyridoxal), 17.9 ng/mL for folate, 1188 pg/mL for vitamin B12, 23.5 μg/mL for vitamin C, 15.0 ng/mL for vitamin D, and 1.03 μg/mL for PIVKA-Ⅱ. An autopsy case of a Japanese middle-aged male with beriberi was also presented, where severe edema and effusion of the cavity and a high NT-proBNP serum value were observed; however, cardiac pathology showed no specific abnormal features. In the present case, the blood vitamin B1 concentration was within the standard clinical range. This study revealed that bloodborne water-soluble vitamin levels shows higher to clinical standard value at postmortem, whereas fat-soluble vitamin levels may stay in the standard range or lower. Our findings suggest that postmortem water-soluble vitamin concentrations within the clinical standard may reflect low antemortem vitamin concentrations.

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引用次数: 0
Age assessment of Chinese Tibetan twins using multiple skeletal age estimation methods: A case report.
IF 1.3 4区 医学 Q3 MEDICINE, LEGAL Pub Date : 2024-12-07 DOI: 10.1016/j.legalmed.2024.102558
Shuai Luo, Fei Fan, Meng Liu, Li-Rong Qiu, Meng-Jun Zhan, Yu-Chi Zhou, Hui-Kun Yang, Hu Chen, Xue-Ling Chen, Zhen-Hua Deng

This case presents a unique age estimation involving Chinese Tibetan twins, where one of them has been arrested for alleged rape. His parents claimed he was about 13 years old without providing any official age documentation. Since age is important in criminal proceedings in China, bone age estimation was conducted to determine the chronological age of the twins. The twins underwent a physical examination, X-rays of the left hand/wrist, and pelvis, and a CT scan of the clavicle. Two age estimation standards in China were used: "Skeletal Maturity and Assessment Methods of Hand and Wrist for Chinese" (China-05 standard), and "Technical Specifications for Skeletal Age Evaluation of Han Population Teenagers" (TSSHPT standard). The bone radiographs showed that most of the twins' epiphyseal development was similar, with only slight differences observed in the distal radius, distal ulna, and iliac crest apophysis. The results of the two methods showed differences in the range of predicted ages. Applying the RUS-CHN method to hand/wrist images, the estimated age of the suspect was determined to be 16-16.5 years old, while his brother's age was 15.5-16 years old. Applying the TSSHPT standard to all three images, the estimated age of the suspect was 17-17.5 years old, while his brother's age was estimated to be 16.5-17 years old. In this case, two commonly used methods for age estimation were introduced. The disparity in age estimations using the RUS-CHN and TSSHPT standards, with the age of the suspect ranging from 16 to 17.5 years. This case revealed that the choice of bone age estimation method can significantly impact the result of estimated age, which has important implications for legal proceedings. Additionally, the minor differences in skeletal maturity between the twins emphasize the need to account for individual variations, even among genetically similar individuals. Furthermore, this case contributes to the broader discourse on the effectiveness of skeletal age estimation methods across diverse populations, underscoring the necessity for tailored evaluation standards that consider genetic and environmental diversity.

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引用次数: 0
Homicide-suicide as domestic violence: A case report with a little literature review.
IF 1.3 4区 医学 Q3 MEDICINE, LEGAL Pub Date : 2024-12-01 DOI: 10.1016/j.legalmed.2024.102557
Beáta Ágnes Borsay, Barbara Dóra Halasi, Róbert Kristóf Pórszász, Péter Attila Gergely

The focus is on unnatural death in forensic pathology including criminal death. One special field is the murder-suicide or homicide-suicide. It is a relatively rare occurrence worldwide mainly with male perpetrators and female victims. The authors report a case about a special type of homicide-suicide which is so-called intimate partner homicide (femicide)-suicide in which the victim was a 42-year-old woman with more than 55 incised- (cuts, stabs, etc.) and blunt force injuries with external examination. The resuscitation was unsuccessful by the paramedics. Not one of the wounds was incompatible with life, some of them had life-threatening conditions, and numerous self-defense type of wounds were observed. Not so far from the crime scene, the 48-year-old perpetrator's hanging corpse was found on a high voltage electric column who previously committed suicide, talked to one of his daughters from his first marriage, left money at his funeral, and asserted he would not have gone to prison again. The authors compared this case with the international literature data.

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引用次数: 0
Effective diagnosis of cervical fracture using postmortem computed tomography and autopsy findings. 利用死后计算机断层扫描和尸检结果有效诊断颈椎骨折。
IF 1.3 4区 医学 Q3 MEDICINE, LEGAL Pub Date : 2024-11-26 DOI: 10.1016/j.legalmed.2024.102555
Yoriko Shinba, Yuki Abe, Takanori Kohyama, Masahide Mitsuma, Hiromi Yamashita, Takehiko Murase, Kazuya Ikematsu

A common forensic problem is cervical fractures, which sometimes need to be diagnosed only by physical examination of the body, without imaging or autopsy. Despite reports from clinical practice describing the association between cervical fractures and head injury, we could not find any analysis of autopsy cases. In addition, discussion of the cervical fracture diagnosis by postmortem computed tomography (PMCT) appears limited. This study aimed to examine autopsy and PMCT findings and explore valid methods for the diagnosis. We analyzed autopsy cases of cervical fractures during the 7 years before (2004-2010) and after (2014-2020) the PMCT introduction in our department. In 2014-2020, 67 autopsy cases with cervical fractures were recorded, of which 61 (91 %) were related to blunt injury to the head. Those with cervical fractures had a significantly higher incidence of blunt injury to the head (p < 0.001), particularly "forehead" and "face" injury, which accounted for > 50 % of cases. Of the external forces on the neck, "extension" accounted for 82.1 %. The cervical fracture-positive rate in all autopsy cases increased significantly from 11 (4.0 %) in 2004-2010 to 67 (8.2 %) in 2014-2020 (p = 0.021). From December 2015 to December 2020, when "cervical retroflexion" imaging was actively performed in PMCT, the cervical fracture diagnosis rate increased significantly from 57.1 % in the cervical normal position to 81.0 % with the addition of "cervical retroflexion" position (p = 0.021). Blunt head injury and PMCT in the "cervical retroflexion" position may be useful in the diagnosis of cervical fractures.

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引用次数: 0
Fatal bear attack in Russian Federation: A case report 俄罗斯联邦致命的熊袭击事件:病例报告
IF 1.3 4区 医学 Q3 MEDICINE, LEGAL Pub Date : 2024-11-26 DOI: 10.1016/j.legalmed.2024.102556
Nikolaos Angelakopoulos , Igor Valentinovich Vlasyuk , Sudheer Babu Balla , Rizky Merdietio Boedi , Galina Zolotenkova
Human-bear conflicts are a growing concern globally, with Russia harboring a significant population of brown bears. This case report details a fatal encounter between a male hunter and a brown bear in the greater Khabarovsk region. On October 2015, the hunter’s body was discovered approximately 400 m from a deceased brown bear, indicating a deadly confrontation. Forensic examination revealed extensive trauma and varied patterns of injuries, with the cause of death attributed to mixed shock from traumatic injuries and acute blood loss. Despite the limited dental information due to the missing maxilla, positive identification was achieved through the unique dental patterns in the victim’s mandible. This study emphasizes the need for specialized forensic knowledge in wildlife-related fatalities, highlighting the importance of accurate post-mortem examinations and the critical role of dental data in positively identifying victims under challenging circumstances.
人熊冲突是全球日益关注的问题,俄罗斯也有大量棕熊栖息。本案例报告详细描述了大哈巴罗夫斯克地区一名男性猎人与棕熊之间的致命遭遇。2015 年 10 月,猎人的尸体在距离一头死亡棕熊约 400 米处被发现,这表明双方发生了致命的冲突。法医检查发现了大面积的创伤和不同的受伤模式,死因是创伤和急性失血造成的混合性休克。尽管由于上颌骨缺失而导致牙齿信息有限,但通过受害者下颌骨上独特的牙齿纹路,还是确定了死者的身份。这项研究强调了在与野生动物有关的死亡事件中对专业法医知识的需求,突出了准确尸检的重要性以及牙科数据在艰难环境下积极识别受害者身份的关键作用。
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引用次数: 0
Immunohistochemical and molecular study for differential diagnosis between freshwater and saltwater drowning 用于鉴别诊断淡水溺水和海水溺水的免疫组化和分子研究。
IF 1.3 4区 医学 Q3 MEDICINE, LEGAL Pub Date : 2024-11-09 DOI: 10.1016/j.legalmed.2024.102545
Rehab A. Azouz , Alaa F. Bakr , Marwa A. Ibrahim , Mohamed Y. Mahmoud
The postmortem identification of drowning in the field of forensic medicine is difficult due to unspecific autopsy findings, and usually, it is a “diagnosis of exclusion”. A model of drowning in salt and fresh water was established to discuss the postmortem changes after drowning and the differences between saltwater drowning (SWD) and freshwater drowning (FWD). The organs (brain and ‘lung) of 30 rats were extracted at three-time points (0 h, 24 h, and 48 h) after drowning. The histopathological, immunohistochemical,l, and molecular changes in the lung and brain of rats at different time points were investigated. Results show no significant difference in pathological findings between fresh and saltwater drowning. Casp3, JNK, and ERK all showed a rise in their postmortem expression in a time-dependent way; the expression of these three genes is much greater in cases of saltwater drowning compared to cases of freshwater drowning. So, it is concluded that after 24 h and 48 h from death, potent cellular oxidative stress occurred and caused the upregulation of the studied genes.
在法医学领域,由于尸检结果不具特异性,溺水的死后鉴定非常困难,通常属于 "排除诊断"。为了讨论溺水后的死后变化以及盐水溺水(SWD)和淡水溺水(FWD)之间的差异,我们建立了盐水和淡水溺水模型。在溺水后的三个时间点(0 h、24 h 和 48 h)提取了 30 只大鼠的器官(脑和肺)。研究了不同时间点大鼠肺和脑的组织病理学、免疫组化和分子变化。结果表明,淡水溺水与盐水溺水的病理结果无明显差异。Casp3、JNK和ERK在大鼠死后的表达均呈上升趋势,且与时间相关;与淡水溺亡相比,盐水溺亡大鼠这三种基因的表达量更高。因此,可以得出结论,在死亡 24 小时和 48 小时后,发生了强烈的细胞氧化应激,导致了所研究基因的上调。
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引用次数: 0
The utility of drowning site inference through metagenomic diatom analysis 通过元基因组硅藻分析推断溺水地点的实用性。
IF 1.3 4区 医学 Q3 MEDICINE, LEGAL Pub Date : 2024-11-01 DOI: 10.1016/j.legalmed.2024.102548
Hiroaki Nakanishi , Aya Takada , Katsumi Yoneyama , Saki Kodama , Kentaro Sakai , Kazuyuki Saito
The diatom test is one of the methods used to diagnose drowning in forensic autopsies. Metagenomic diatom analysis may reveal where a drowning occurred. We evaluated whether metagenomic diatom analysis could be used to infer waters, watersheds, and geographic locations using 166 water samples from 64 locations (freshwater: 55; seawater: 9). Principal component analysis (PCA) in all samples revealed no specific clusters for waters or watersheds. In one river, the three samples at the same site generally tended to be in close clusters, but there were some cases where the three sites were far from each other. The precise geographic location could thus not be reliably identified. However, PCA of data from dams, lakes, and retention basins revealed sites with independent clusters, suggesting unique diatom compositions. Diatoms of seawater were not detected in freshwater. The high number of Actinoptychus, Chaetoceros, and Skeletonema detected in seawater samples suggested that they are useful for seawater identification. This method required only 2 mL of water; it suggests that this method can be applied to actual samples. In summary, it was difficult to infer the geographic location and waters or watersheds, but the freshwater/seawater distinction could be easily made, and depending on the application, it may be useful in forensic science practice.
硅藻测试是法医验尸中诊断溺水的方法之一。元基因组硅藻分析可以揭示溺水发生的地点。我们利用来自 64 个地点(淡水:55 个;海水:9 个)的 166 个水样,评估了元基因组硅藻分析是否可用于推断水域、流域和地理位置。对所有样本进行的主成分分析(PCA)显示,水域或流域没有特定的群集。在一条河流中,同一地点的三个样本一般倾向于处于相近的群组中,但也有三个地点相距较远的情况。因此,无法可靠地确定准确的地理位置。不过,对来自水坝、湖泊和蓄水池的数据进行 PCA 分析后发现,这些地点具有独立的聚类,表明硅藻组成具有独特性。淡水中未检测到海水硅藻。在海水样本中检测到大量的 Actinoptychus、Chaetoceros 和 Skeletonema,这表明它们可用于海水鉴定。该方法仅需 2 毫升水,这表明该方法可用于实际样本。总之,很难推断地理位置和水域或流域,但很容易区分淡水和海水,根据应用情况,它可能在法医学实践中有用。
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引用次数: 0
Research progress and application of the third-generation sequencing technologies in forensic medicine 第三代测序技术在法医学中的研究进展和应用
IF 1.3 4区 医学 Q3 MEDICINE, LEGAL Pub Date : 2024-11-01 DOI: 10.1016/j.legalmed.2024.102532
Xiaoxin Hu , Jinjie Liu , Tingyu Xu , Kaiyue Qin , Yunpeng Feng , Zhenjun Jia , Xingchun Zhao
Third-generation sequencing technologies, exemplified by single-molecule real-time sequencing and nanopore sequencing, provide a constellation of advantages, including long read lengths, high throughput, real-time sequencing capabilities, and remarkable portability. These cutting-edge methodologies have provided new tools for genomic analysis in forensic medicine. To gain a comprehensive understanding of the current applications and cutting-edge trends of third-generation sequencing technologies in forensic medicine, this study retrieved relevant literature from the China National Knowledge Infrastructure (CNKI) database and the Web of Science (WOS) database. Using bibliometric software CiteSpace 6.1.R6, the study visualized publication volume, countries, and keywords related to the application of third-generation sequencing technologies in forensic medicine from 2014 to 2023. The review then summarized the foundational principles, characteristics, and promising prospects of third-generation sequencing technologies in forensic medicine. Notably, it highlights their remarkable contributions in forensic individual identification, body fluid identification, forensic epigenetic analysis, microbial analysis and forensic species identification.
以单分子实时测序和纳米孔测序为代表的第三代测序技术具有多种优势,包括长读取长度、高通量、实时测序能力和出色的便携性。这些尖端方法为法医学基因组分析提供了新工具。为了全面了解第三代测序技术在法医学中的应用现状和前沿趋势,本研究从中国国家知识基础设施(CNKI)数据库和科学网(WOS)数据库中检索了相关文献。研究使用文献计量软件CiteSpace 6.1.R6,对2014年至2023年与第三代测序技术在法医学中的应用相关的发表量、国家和关键词进行了可视化分析。然后,综述总结了第三代测序技术在法医学中的基本原理、特点和广阔前景。特别是,它强调了第三代测序技术在法医个体鉴定、体液鉴定、法医表观遗传分析、微生物分析和法医物种鉴定方面的突出贡献。
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引用次数: 0
Complete aortic rupture following wakeboarding accident 滑水事故后主动脉完全破裂。
IF 1.3 4区 医学 Q3 MEDICINE, LEGAL Pub Date : 2024-11-01 DOI: 10.1016/j.legalmed.2024.102547
Alberto Amadasi, Larissa Amadasi
Wakeboarding is a sport associated with various types of injuries, primarily affecting the upper and lower limbs. In this case, a 44-year-old man fell from a ramp while wakeboarding and barely managed to reach the shore before dying shortly afterward. An autopsy revealed a complete rupture of the thoracic aorta along with a fracture of the fourth thoracic vertebra. It is likely that several mechanisms contributed to these injuries, either individually or in combination: concussive, rotational, and tensile forces. Additionally, it is plausible that the rupture occurred in two stages, with an initial partial injury worsening during subsequent movements or attempts at rescue and resuscitation. This is the first reported case of death resulting from a ruptured aorta due to wakeboarding, highlighting a potential consequence of this sport.
滑水是一项与各种类型的伤害有关的运动,主要影响上肢和下肢。在本案中,一名 44 岁的男子在滑水时从斜坡上摔下,勉强爬上岸后不久便死亡。尸检显示胸主动脉完全断裂,第四节胸椎骨折。造成这些伤害的机制可能有几种,有的是单独造成的,有的是共同造成的:震荡力、旋转力和拉力。此外,破裂可能是分两个阶段发生的,最初的部分损伤在随后的运动或抢救和复苏过程中恶化。这是报告的首例因滑水导致主动脉破裂而死亡的病例,凸显了这项运动的潜在后果。
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引用次数: 0
Evaluation of two scoring systems assessing the epiphyseal union at shoulder joint as predictors of chronological age among a sample of Egyptians 评估肩关节骨骺结合的两种评分系统作为埃及人样本中预测年龄的指标
IF 1.3 4区 医学 Q3 MEDICINE, LEGAL Pub Date : 2024-11-01 DOI: 10.1016/j.legalmed.2024.102546
Asmaa F. Sharif , Hadeel Eid , Mahmoud Abdelaziz Abdelnaby Ghalab , Asmaa Ali Ahmed Elfeky , Mohamed Moharram Badawy , Nagwa Mahmoud Habib , Reham Hassan El-Farouny , Heba A.A. Mabrouk
Age estimation has extensive medicolegal implications in civil and criminal identification. Despite the surge in adopting radiological investigations to assess developmental bony changes, the shoulder joint is understudied. A cross-sectional study was conducted, enrolling 283 shoulder radiographs of Egyptians, investigating the reliability of two previously established scores as predictors of chronological age using the epiphyseal maturation of proximal humerus and acromion process. Epiphyseal union of proximal humerus commenced at age of 16.1–17 and completed around 21, while complete acromial union was observed around the age of 20.8. Females significantly preceded males and showed lower mean total Scores A and B at different maturation stages. There was a significant strong positive correlation between the chronological age and the epiphyseal maturation of humerus, acromion and total shoulder scores with correlation coefficients between 0.84 and 0.9. The receiver operating characteristic curves showed significant discriminating power of the total shoulder Scores A and B as predictors of the ages of 14 and 16, with area under curves above 0.9, minimal accuracy of 96.5 % and p values of 0.001. Six proposed models were established where the model “age = 0.318 + (0.388) total shoulder Score A + (2.842) total shoulder Score B + 1.931 (sex)” showed the best significant prediction power of radiographic evaluation of epiphyseal maturation in the proximal humerus and acromion in estimating the ages between 8 and around 20 years (R2 of 0.812). Applying this model to assess the chronological age, especially if the results from the hand and teeth are inconclusive, is promising.
年龄估计在民事和刑事鉴定中具有广泛的医学法律意义。尽管采用放射学检查来评估骨骼发育变化的趋势日益明显,但对肩关节的研究却不足。这项横断面研究收集了 283 张埃及人的肩部 X 光片,利用肱骨近端和肩峰突的骨骺成熟情况,对之前确定的两个预测年龄的分数的可靠性进行了调查。肱骨近端骺端结合始于 16.1-17 岁,完成于 21 岁左右,而肩峰完全结合则是在 20.8 岁左右。女性明显早于男性,且在不同成熟阶段的平均总分 A 和 B 都较低。计时年龄与肱骨、肩峰的骨骺成熟度和肩关节总分之间存在明显的正相关,相关系数在 0.84 和 0.9 之间。接受者操作特征曲线显示,肩关节总评分 A 和 B 对 14 岁和 16 岁的预测具有显著的鉴别力,曲线下面积大于 0.9,最小准确率为 96.5 %,P 值为 0.001。我们建立了六个拟议模型,其中 "年龄 = 0.318 + (0.388) 肩部总分 A + (2.842) 肩部总分 B + 1.931(性别)"模型显示,肱骨近端和肩峰骺端骨骺成熟度的影像学评估对估计 8 岁至 20 岁左右的年龄具有最佳的显著预测能力(R2 为 0.812)。应用该模型评估法定年龄,尤其是在手部和牙齿的评估结果不确定的情况下,是很有前景的。
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引用次数: 0
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Legal Medicine
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