Pub Date : 2023-05-31DOI: 10.1515/labmed-2022-0150
Andrei A. Svobodov, A. A. Kupryashov, Tatayna K. Dobroserdova, E. Levchenko, M. Tumanyan, Aleksei G. Anderson
Abstract Objectives BNP is the unique cardiac marker that reflects not as much as the degree of heart muscle damage, but mostly the severity of hemodynamic disorder, which is important in congenital heart disease. The only disadvantage of this marker is the barely studied reference values for children. It is known that the younger the child is, the higher the BNP value will be. By shifting from interpreting the absolute values towards the application of zlog-transformed data in clinical practice, we can overcome the above problems. Methods We performed an age-adjusted zlog transformation of BNP concentration. The age dependence was accounted for by a piecewise linear interpolation of the logarithms of BNP concentration among healthy children in different age groups from the logarithms of age. Results The concentration of BNP was measured in 351 patients (under 1 year old) with various heart diseases. The median age at the time of testing was 52 days [10; 166]; the median weight was 4.1 kg [3.2; 6.2]. The conditions we investigated included almost all known congenital heart diseases, as well as primary cardiac tumors. After the zlog transformation, we eliminated age-dependence, which was proved by comparing BNP concentrations in two groups of patients with univentricular and biventricular hemodynamics. Conclusions BNP in patients with congenital heart disease reflects the severity of hemodynamic disorders, and zlogBNP is an objective, age-independent and clear mechanism that can be used to interpret this cardiac marker.
BNP是一种独特的心脏标志物，不仅能反映心肌损伤的程度，还能反映血流动力学障碍的严重程度，这在先天性心脏病中很重要。这个标记唯一的缺点是对儿童的参考值几乎没有研究。已知儿童年龄越小，BNP值越高。从对绝对值的解释转向对经zlog变换后的数据在临床实践中的应用，可以克服上述问题。方法对BNP浓度进行年龄调整后的zlog变换。不同年龄组健康儿童BNP浓度的对数与年龄对数的分段线性插值解释了年龄依赖性。结果对351例年龄在1岁以下 的心脏病患者进行了BNP浓度测定。检测时的中位年龄为52 天[10;166);中位体重为4.1 kg [3.2;6.2]。我们调查的情况包括几乎所有已知的先天性心脏病，以及原发性心脏肿瘤。zlog转换后，我们消除了年龄依赖性，通过比较两组单室和双室血流动力学患者的BNP浓度证明了这一点。结论先天性心脏病患者的BNP反映了血流动力学障碍的严重程度，zlogBNP是一个客观的、与年龄无关的、明确的机制，可以用来解释这一心脏标志物。
{"title":"A new approach to the interpretation of B-type natriuretic peptide concentration in children with congenital heart disease","authors":"Andrei A. Svobodov, A. A. Kupryashov, Tatayna K. Dobroserdova, E. Levchenko, M. Tumanyan, Aleksei G. Anderson","doi":"10.1515/labmed-2022-0150","DOIUrl":"https://doi.org/10.1515/labmed-2022-0150","url":null,"abstract":"Abstract Objectives BNP is the unique cardiac marker that reflects not as much as the degree of heart muscle damage, but mostly the severity of hemodynamic disorder, which is important in congenital heart disease. The only disadvantage of this marker is the barely studied reference values for children. It is known that the younger the child is, the higher the BNP value will be. By shifting from interpreting the absolute values towards the application of zlog-transformed data in clinical practice, we can overcome the above problems. Methods We performed an age-adjusted zlog transformation of BNP concentration. The age dependence was accounted for by a piecewise linear interpolation of the logarithms of BNP concentration among healthy children in different age groups from the logarithms of age. Results The concentration of BNP was measured in 351 patients (under 1 year old) with various heart diseases. The median age at the time of testing was 52 days [10; 166]; the median weight was 4.1 kg [3.2; 6.2]. The conditions we investigated included almost all known congenital heart diseases, as well as primary cardiac tumors. After the zlog transformation, we eliminated age-dependence, which was proved by comparing BNP concentrations in two groups of patients with univentricular and biventricular hemodynamics. Conclusions BNP in patients with congenital heart disease reflects the severity of hemodynamic disorders, and zlogBNP is an objective, age-independent and clear mechanism that can be used to interpret this cardiac marker.","PeriodicalId":49926,"journal":{"name":"Laboratoriumsmedizin-Journal of Laboratory Medicine","volume":"43 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86647016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-19DOI: 10.1515/labmed-2023-0021
Jakob Adler, M. Lenski, A. Tolios, S. Taie, Miron Sopić, D. Rajdl, A. Rampul, G. Sancesario, R. Biemann
Abstract Objectives Even though most physicians and professionals in laboratory medicine have received basic training in statistics, experience shows that a general understanding of data analysis is not yet available on a broad scale. Therefore, data literacy, data-driven decision making, and computational thinking should be implemented in future educational training. To evaluate the state of digital competence among young scientists (YS) in laboratory medicine, we launched a worldwide online survey. Methods A global online survey was conducted from 25/05/2022 to 26/06/2022 and was disseminated to YS who are listed in three large networks: YS of the DGKL, the EFLM Task Group-YS, and IFCC Task Force-YS and its corresponding members, covering a base of 53 countries. Results 119 young scientists from 40 countries participated in this survey. 80 % did not learn digital skills in their academic education but 96 % felt they needed to. Digital literacy was associated with terms such as programming, artificial intelligence and machine learning, statistics, communication, Big Data and data analytics. Conclusions The results of our survey show that more knowledge and training in the area of digital skills is not just necessary, but also wanted by young scientists. A varied learning environment consisting of tutorial articles, videos, exercises, technical articles, collection of helpful links, online meetings and in person bootcamps is crucial to meet the challenges of an international project with different languages, health systems and time zones.
{"title":"Digital competence in laboratory medicine","authors":"Jakob Adler, M. Lenski, A. Tolios, S. Taie, Miron Sopić, D. Rajdl, A. Rampul, G. Sancesario, R. Biemann","doi":"10.1515/labmed-2023-0021","DOIUrl":"https://doi.org/10.1515/labmed-2023-0021","url":null,"abstract":"Abstract Objectives Even though most physicians and professionals in laboratory medicine have received basic training in statistics, experience shows that a general understanding of data analysis is not yet available on a broad scale. Therefore, data literacy, data-driven decision making, and computational thinking should be implemented in future educational training. To evaluate the state of digital competence among young scientists (YS) in laboratory medicine, we launched a worldwide online survey. Methods A global online survey was conducted from 25/05/2022 to 26/06/2022 and was disseminated to YS who are listed in three large networks: YS of the DGKL, the EFLM Task Group-YS, and IFCC Task Force-YS and its corresponding members, covering a base of 53 countries. Results 119 young scientists from 40 countries participated in this survey. 80 % did not learn digital skills in their academic education but 96 % felt they needed to. Digital literacy was associated with terms such as programming, artificial intelligence and machine learning, statistics, communication, Big Data and data analytics. Conclusions The results of our survey show that more knowledge and training in the area of digital skills is not just necessary, but also wanted by young scientists. A varied learning environment consisting of tutorial articles, videos, exercises, technical articles, collection of helpful links, online meetings and in person bootcamps is crucial to meet the challenges of an international project with different languages, health systems and time zones.","PeriodicalId":49926,"journal":{"name":"Laboratoriumsmedizin-Journal of Laboratory Medicine","volume":"353 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76473065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-04DOI: 10.1515/labmed-2022-0125
{"title":"German Congress of Laboratory Medicine: 17th Annual Congress of the DGKL and 4th Symposium of the Biomedical Analytics of the DVTA e. V","authors":"","doi":"10.1515/labmed-2022-0125","DOIUrl":"https://doi.org/10.1515/labmed-2022-0125","url":null,"abstract":"","PeriodicalId":49926,"journal":{"name":"Laboratoriumsmedizin-Journal of Laboratory Medicine","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72655601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-11-15DOI: 10.1515/labmed-2021-0159
Adenosine Deaminase 2 Deficiency (DADA2) (OMIM: 607575) is a monogenic autoinflammatory disease caused by loss of function homozygous or heterozygous mutations in ADA 2 gene (previously CECR1, Cat Eye Syndrome Chromosome Region 1. A timely diagnosis is crucial to start Anti-TNF therapies that are efficacious in controlling the disease. The confirmation of DADA2 is based on DNA sequencing and enzymatic assay. It is thus very important to have robust and reliable assays that can be rapidly utilized in specialized laboratories that can centralize samples from other centers. In this paper we show a novel enzymatic assay based on liquid chromatography-tandem mass spectrometry that allows the accurate determination of the ADA2 enzyme activity starting from very small amounts of plasma spotted on filter paper (dried plasma spot). ADA2 activity was determined in dried plasma spots (DPS) from 44 healthy donors, 18 DADA2 patients and 4 carriers. ADA2 expressed mean ± ± ± ± WHO has described malnutrition as a “ global problem ” , having an adverse effect on the survival, health performance, and progression of the population group. It is highly prevalent in developing countries among children below the age of 5 years; with severe forms occurring in 1-10% and underweight observed in 20-40%. The aim of the study was to evaluate copper and zinc levels in children with protein-energy malnutrition. Serum zinc and copper were determined in thirty (30) malnourished pre-school-age children (age, 1-5 years) and thirty (30) age-and sex-matched apparently healthy well-nourished controls to evaluate the effect of protein- energy malnutrition on serum zinc and copper. Serum zinc and copper concentrations were estimated by the Atomic Absorption Spectrophotometer. BACKGROUND-AIM Multisystem Inflammatory Syndrome in Children (MIS-C) associated with Coronavirus Disease (COVID-19) is a serious condition among paediatric patients, usually under 19 years, with previous exposure to SARS-CoV-2. Clinical manifestations consist of persistent fever along with weakness, abdominal pain, vomiting and/or diarrhea and skin rashes. Although this syndrome is rare and has variable expressivity, some children can evolve from hypotension and cardiogenic shock to multiple organ dysfunction. Laboratory abnormalities include elevation of acute phase reactants and myocardial dysfunction; similar to those observed in Kawasaki disease or toxic shock syndrome, requiring a differential diagnosis. RT-PCR respiratory viruses (SARS-CoV-2, RSV, H. in fl uenzae ), blood and urine cultures: negative. RESULTS Elevation of inflammatory markers (C-reactive protein 282 mg/L, ferritin 1103 ng/mL, IL-6 152 pg/mL, fi brinogen 667 mg/dL, PCT 6.56 ng/mL); cardiac markers (NT-proBNP 966 pg/mL); D-dimer 1120 ng/mL and renal and liver function tests (creatinine 1,04 mg/dL and GPT 44 U/L). Decrease in sodium (130 Congenital Disorders of Glycosylation (CDG) are a rapidly expanding family of rare inborn errors of metabolis
{"title":"XVth International Congress on Pediatric Laboratory Medicine, Munich, Nov 27–28, 2021; Poster Presentation Abstracts","authors":"","doi":"10.1515/labmed-2021-0159","DOIUrl":"https://doi.org/10.1515/labmed-2021-0159","url":null,"abstract":"Adenosine Deaminase 2 Deficiency (DADA2) (OMIM: 607575) is a monogenic autoinflammatory disease caused by loss of function homozygous or heterozygous mutations in ADA 2 gene (previously CECR1, Cat Eye Syndrome Chromosome Region 1. A timely diagnosis is crucial to start Anti-TNF therapies that are efficacious in controlling the disease. The confirmation of DADA2 is based on DNA sequencing and enzymatic assay. It is thus very important to have robust and reliable assays that can be rapidly utilized in specialized laboratories that can centralize samples from other centers. In this paper we show a novel enzymatic assay based on liquid chromatography-tandem mass spectrometry that allows the accurate determination of the ADA2 enzyme activity starting from very small amounts of plasma spotted on filter paper (dried plasma spot). ADA2 activity was determined in dried plasma spots (DPS) from 44 healthy donors, 18 DADA2 patients and 4 carriers. ADA2 expressed mean ± ± ± ± WHO has described malnutrition as a “ global problem ” , having an adverse effect on the survival, health performance, and progression of the population group. It is highly prevalent in developing countries among children below the age of 5 years; with severe forms occurring in 1-10% and underweight observed in 20-40%. The aim of the study was to evaluate copper and zinc levels in children with protein-energy malnutrition. Serum zinc and copper were determined in thirty (30) malnourished pre-school-age children (age, 1-5 years) and thirty (30) age-and sex-matched apparently healthy well-nourished controls to evaluate the effect of protein- energy malnutrition on serum zinc and copper. Serum zinc and copper concentrations were estimated by the Atomic Absorption Spectrophotometer. BACKGROUND-AIM Multisystem Inflammatory Syndrome in Children (MIS-C) associated with Coronavirus Disease (COVID-19) is a serious condition among paediatric patients, usually under 19 years, with previous exposure to SARS-CoV-2. Clinical manifestations consist of persistent fever along with weakness, abdominal pain, vomiting and/or diarrhea and skin rashes. Although this syndrome is rare and has variable expressivity, some children can evolve from hypotension and cardiogenic shock to multiple organ dysfunction. Laboratory abnormalities include elevation of acute phase reactants and myocardial dysfunction; similar to those observed in Kawasaki disease or toxic shock syndrome, requiring a differential diagnosis. RT-PCR respiratory viruses (SARS-CoV-2, RSV, H. in fl uenzae ), blood and urine cultures: negative. RESULTS Elevation of inflammatory markers (C-reactive protein 282 mg/L, ferritin 1103 ng/mL, IL-6 152 pg/mL, fi brinogen 667 mg/dL, PCT 6.56 ng/mL); cardiac markers (NT-proBNP 966 pg/mL); D-dimer 1120 ng/mL and renal and liver function tests (creatinine 1,04 mg/dL and GPT 44 U/L). Decrease in sodium (130 Congenital Disorders of Glycosylation (CDG) are a rapidly expanding family of rare inborn errors of metabolis","PeriodicalId":49926,"journal":{"name":"Laboratoriumsmedizin-Journal of Laboratory Medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79783052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"German Congress of Laboratory Medicine: 15th Annual Congress of the DGKL (German Society of Clinical Chemistry and Laboratory Medicine) and the 3rd Symposium on Biomedical Analysis","authors":"S. Sollfrank, Stefano Barco, A. Trinchero, L. Tomao, B. Zieger, J. Hovinga, Laura Conti, Anke, Adenäuer, E. Miloni, Karl Lackner, H. Rossmann","doi":"10.1515/labmed-2018-0115","DOIUrl":"https://doi.org/10.1515/labmed-2018-0115","url":null,"abstract":"s*) German Congress of Laboratory Medicine: 15th Annual Congress of the DGKL (German Society of Clinical Chemistry and Laboratory Medicine) and the 3rd Symposium on Biomedical Analysis Mannheim, Germany, September 26–29, 2018 Under the auspices of International Federation of Clinical Chemistry and Laboratory Medicine European Federation of Clinical Chemistry and Laboratory Medicine Congress Presidents Eberhard Wieland (Stuttgart, Germany) Matthias Orth (Stuttgart, Germany) Hansjörg Baum (Ludwigsburg, Germany) Christiane Maschek (DVTA, Hamburg, Germany) Sponsor of Abstract awards Dr. Neumann & Kindler Ltd. & Co. KG (Bochum, Germany) Scientific Committee Ahmad-Nejad Parviz; Aufenanger, Johannes; Bauer Matthias; Baum Hannsjörg; Bertsch Thomas; Birschmann Ingvild; Bobrowski Andreas; Brand Korbinian; Bühling Frank; Burkhardt Ralph; Ceglarek Uta; Chavakis Triantafyllos; Danckwardt Sven; Fischer Andreas; Fraunberger Peter; Gässler Norbert; Gerritzen Andreas; Griesmacher Andrea; Gurr Eberhard; Häcker Georg; Haferlach Thorsten; Hallbach Jürgen; Haselmann Verena; Haushofer Alexander; Heeg Klaus; Hersberger Martin; Hoffmann Georg; Hofmann Walter; Holdenrieder Stefan; Hunfeld Klaus-Peter; Isermann Berend; Junker Ralf; Kaap-Fröhlich Sylvia; Kachler Marco; Kessler Harald; Kiehntopf Michael; Klouche Mariam; Knabbe Cornelius; Kohse Klaus P.; Kratzsch Jürgen; Lackner Karl; Lichtinghagen Ralf; Luppa Peter; Magnussen Karin; Maschek Christiane; Miethke Thomas; Nauck Matthias; Neumaier Michael; Nofer Jerzy-Roch; Orth Matthias; Peetz Dirk; Peter Andreas; Petersmann Astrid; Rauh Manfred; Renné Thomas; Renz Harald; Ruland Jürgen; Schimanski Sven; Schuff-Werner Peter; Siegert Gabriele; Stoffel-Wagner Birgit; Streichert Thomas; Tauber Rudolf; Teupser Daniel; Uhr Manfred; Vogeser Michael; von Ahsen Nicolas; von Eckardstein Arnold; Walter Ulrich; Walter Philipp; Wiegel Bernhard; Wieland Eberhard *)These abstracts have been reproduced directly from the material supplied by the authors, without editorial alteration by the staff of this Journal. Insufficiencies of preparation, grammar, spelling, style, syntax, and usage are the authors. Unauthenticated Download Date | 11/1/18 3:56 PM eA2 15th DGKL Annual Congress, Mannheim, Germany, September 26–29, 2018","PeriodicalId":49926,"journal":{"name":"Laboratoriumsmedizin-Journal of Laboratory Medicine","volume":"151 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77037023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-12-20DOI: 10.1515/labmed-2017-0030
F. Masjedian Jazi, R. Mirnejad, Vahhab Piranfar, Noor Amir Mozafari, T. Zahraei Salehi, M. Khormali, M. Sedighi, G. Irajian
Abstract Background: It is of great importance to quickly and accurately detect Brucella abortus and Brucella melitensis from clinical and non-clinical samples because of their high prevalence and high risk in causing brucellosis, a life-threating infectious disease affecting both humans and animals. Methods: The current study describes a new method for the detection of brucellosis in clinical samples using real-time polymerase chain reaction (PCR) and high-resolution melt (HRM) curve analysis. This study was conducted on 70 human and 55 animal isolates with more than 1/80 serum antibody titers. Additionally, the accuracy and specificity of the methods were compared. Results: The mean range [cycles threshold±standard deviation (CT±SD)] for the amplified samples was 15.39–25.15 by real-time PCR. The melting peak range (°C) ±SD for B. abortus and B. melitensis was 90.10±0.4 and 89.70±0.4, respectively, and 10 was reported on peak height. Conclusions: The results of HRM analysis can be used for species differentiation and bacterial genotyping according to nucleotide polymorphism. This molecular method could help in diagnosing Brucella quickly and precisely. Quick recognition of Brucella species could decrease its prevalence among humans and animals and mitigate economic loss.
{"title":"Real-time PCR and high-resolution melt analysis methods for detection of pathogenic species of Brucella","authors":"F. Masjedian Jazi, R. Mirnejad, Vahhab Piranfar, Noor Amir Mozafari, T. Zahraei Salehi, M. Khormali, M. Sedighi, G. Irajian","doi":"10.1515/labmed-2017-0030","DOIUrl":"https://doi.org/10.1515/labmed-2017-0030","url":null,"abstract":"Abstract Background: It is of great importance to quickly and accurately detect Brucella abortus and Brucella melitensis from clinical and non-clinical samples because of their high prevalence and high risk in causing brucellosis, a life-threating infectious disease affecting both humans and animals. Methods: The current study describes a new method for the detection of brucellosis in clinical samples using real-time polymerase chain reaction (PCR) and high-resolution melt (HRM) curve analysis. This study was conducted on 70 human and 55 animal isolates with more than 1/80 serum antibody titers. Additionally, the accuracy and specificity of the methods were compared. Results: The mean range [cycles threshold±standard deviation (CT±SD)] for the amplified samples was 15.39–25.15 by real-time PCR. The melting peak range (°C) ±SD for B. abortus and B. melitensis was 90.10±0.4 and 89.70±0.4, respectively, and 10 was reported on peak height. Conclusions: The results of HRM analysis can be used for species differentiation and bacterial genotyping according to nucleotide polymorphism. This molecular method could help in diagnosing Brucella quickly and precisely. Quick recognition of Brucella species could decrease its prevalence among humans and animals and mitigate economic loss.","PeriodicalId":49926,"journal":{"name":"Laboratoriumsmedizin-Journal of Laboratory Medicine","volume":"36 3 1","pages":"325 - 331"},"PeriodicalIF":0.0,"publicationDate":"2017-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91068909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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