In this article, we give the notion of left restriction meet-semigroup, and establish some results regarding atomistic left restriction semigroups. Then we discuss decompositions of (non-zero) semigroups with zero by proving a decomposition theorem. We also show that every atomistic left restriction semigroup S can be decomposed as an orthogonal sum of atomistic left restriction semigroups Ni, where each summand Ni is an irreducible ideal of S. Finally, properties of the summands Ni, when S embeds in some PT X the partial transformation monoid on a set X, are investigated.
{"title":"On left restriction semigroups with zero","authors":"Baddi-Ul Zaman","doi":"10.48129/kjs.16921","DOIUrl":"https://doi.org/10.48129/kjs.16921","url":null,"abstract":"In this article, we give the notion of left restriction meet-semigroup, and establish some results regarding atomistic left restriction semigroups. Then we discuss decompositions of (non-zero) semigroups with zero by proving a decomposition theorem. We also show that every atomistic left restriction semigroup S can be decomposed as an orthogonal sum of atomistic left restriction semigroups Ni, where each summand Ni is an irreducible ideal of S. Finally, properties of the summands Ni, when S embeds in some PT X the partial transformation monoid on a set X, are investigated.","PeriodicalId":49933,"journal":{"name":"Kuwait Journal of Science & Engineering","volume":"64 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89954530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. K. Ali, J. Khan, M. S. Mughal, Muhammad R. x Muhammad R. Lashari, A. G. Sahito, Fahad Hameed, Hafiz S. Bashir, Ahmer Bilal, S. Razzaq
The Khairi Murat-Kauliar area lies between the Salt Range Thrust (SRT) in the south whereas Main Boundary Thrust (MBT) in the north which is the part of the Potwar Sub Basin. Eocene to Recent succession is exposed in the study area. The selected siliciclastic Siwalik Group display various facies in a fluviatile environment, with different lithologies, and sedimentary features. The petrographic studies of Siwalik Group delineate different microfacies including feldspathic litharenite, litharenite and sub arkose. The provenance study of sandstones depicted the terrane of dissected arc and recycled orogeny, indicating that the detritus was received from sedimentary, metamorphic, plutonic and volcanic rocks from the northern domain of Indian Plate, Karakoram Ranges and Kohistan Island arc. The Siwalik sandstone also contains heavy mineral assemblage such as tourmaline, garnet, epidote, hornblende and chlorite. This heavy detritus assemblage depicted that the deposits were received from metamorphic and igneous origin. A modified dynamic depositional stages of the Siwalik Group with respect to Himalayan orogeny is further built using outcrop data, microfacies and heavy minerals analysis. According to this concept, the group was deposited along Himalayan uprising and thrusting, on the depository in targeted area. The Siwalik Group depicted different phase of Himalayan tectonics and these molasse sequence were deposited in a subsiding foreland basin under the conditions of rapid erosion and quick deposition.
{"title":"Petrotectonic framework of Siwalik Group in Khairi Murat-Kauliar area, Potwar Sub-Basin, Pakistan","authors":"S. K. Ali, J. Khan, M. S. Mughal, Muhammad R. x Muhammad R. Lashari, A. G. Sahito, Fahad Hameed, Hafiz S. Bashir, Ahmer Bilal, S. Razzaq","doi":"10.48129/kjs.17577","DOIUrl":"https://doi.org/10.48129/kjs.17577","url":null,"abstract":"The Khairi Murat-Kauliar area lies between the Salt Range Thrust (SRT) in the south whereas Main Boundary Thrust (MBT) in the north which is the part of the Potwar Sub Basin. Eocene to Recent succession is exposed in the study area. The selected siliciclastic Siwalik Group display various facies in a fluviatile environment, with different lithologies, and sedimentary features. The petrographic studies of Siwalik Group delineate different microfacies including feldspathic litharenite, litharenite and sub arkose. The provenance study of sandstones depicted the terrane of dissected arc and recycled orogeny, indicating that the detritus was received from sedimentary, metamorphic, plutonic and volcanic rocks from the northern domain of Indian Plate, Karakoram Ranges and Kohistan Island arc. The Siwalik sandstone also contains heavy mineral assemblage such as tourmaline, garnet, epidote, hornblende and chlorite. This heavy detritus assemblage depicted that the deposits were received from metamorphic and igneous origin. A modified dynamic depositional stages of the Siwalik Group with respect to Himalayan orogeny is further built using outcrop data, microfacies and heavy minerals analysis. According to this concept, the group was deposited along Himalayan uprising and thrusting, on the depository in targeted area. The Siwalik Group depicted different phase of Himalayan tectonics and these molasse sequence were deposited in a subsiding foreland basin under the conditions of rapid erosion and quick deposition.","PeriodicalId":49933,"journal":{"name":"Kuwait Journal of Science & Engineering","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75392587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study is based on 93 Spinturnicidae specimens collected on 312 bat specimens obtained from various parts of Turkey. As a result of the study, six mite species were identified: Eyndhovenia euryalis (Canestrini, 1884), Spinturnix acuminatus (Koch, 1836), S. myoti (Kolenati, 1856), S. psi (Kolenati, 1856), S. plecotinus (Koch, 1839), and S. punctata (Sundevall, 1833). Of these species, S. plecotinus and S. punctata are new records for the Turkish fauna.
{"title":"The wing mites (Acari: Spinturnicidae) of the Turkish bats, including new records","authors":"A. Karataş, Ferhat Toprak","doi":"10.48129/kjs.11569","DOIUrl":"https://doi.org/10.48129/kjs.11569","url":null,"abstract":"This study is based on 93 Spinturnicidae specimens collected on 312 bat specimens obtained from various parts of Turkey. As a result of the study, six mite species were identified: Eyndhovenia euryalis (Canestrini, 1884), Spinturnix acuminatus (Koch, 1836), S. myoti (Kolenati, 1856), S. psi (Kolenati, 1856), S. plecotinus (Koch, 1839), and S. punctata (Sundevall, 1833). Of these species, S. plecotinus and S. punctata are new records for the Turkish fauna.","PeriodicalId":49933,"journal":{"name":"Kuwait Journal of Science & Engineering","volume":"191 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84184046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Arshad, Gulzar Akhtar, I. A. Rajwana, S. Ullah, M. Hussain, M. Amin, Nazar Faried, K. Razzaq, M. Shehzad, M. Ahsan, Y. Sajjad, I. Ahmed
Rose is an important floricultural crop that has been exploited for many uses. Its important uses in different industries include, pharmaceutical, perfumery, and food industries that manifest higher flower yield. Therefore, response of Gruss-an-Taplitz to foliar application of chitosan (Ct) solution (0, 2.5, 5, 7.5, 10 mg L-1), was evaluated in an experimental field. Ct treatment had significant effects on studied parameters, including plant growth, pigments, enzymes, and gaseous exchange. This experiment was laid out under Randomized Complete Block Design (RCBD) using three replications per treatment. Ct (7.5 mg L-1) significantly improved growth, in terms of higher leaf area (20.37%), plant height (20.19%), number of flowers (55.51%), flower weight (34.64%) and flower diameter (33.78%) as well as enhancing relative water contents (27.38%) with respect to control. Chlorophyll a (54.60%), Chlorophyll b (12.13%), Carotenoid (8.36%) and anthocyanins (17.09%) were also increased at 7.5 mg L-1 Ct, which showed higher photosynthetic pigments as compared to control. Consequently, Ct (7.5 mg L-1) treated plants showed higher enzymatic activity; CAT (9.94%), SOD (83.87%), POD (64.54%), total antioxidant (35.48%), phenolics (7.41%) and gaseous exchange; Pn (55.65%), E (31.76%), and gs (18.38%) Ci (34.17%) that improved the plant growth and productivity of Gruss-an-Taplitz. Foliar application of 7.5 mg L-1 Ct improved biomass, water preservation, pigments, enzymatic activity and leaf gaseous exchange, resulted in higher production and quality of Gruss-an-Taplitz plants.
{"title":"Foliar application of chitosan improves plant biomass, physiological and biochemical attributes of rose (Gruss-an-Teplitz)","authors":"M. Arshad, Gulzar Akhtar, I. A. Rajwana, S. Ullah, M. Hussain, M. Amin, Nazar Faried, K. Razzaq, M. Shehzad, M. Ahsan, Y. Sajjad, I. Ahmed","doi":"10.48129/kjs.11655","DOIUrl":"https://doi.org/10.48129/kjs.11655","url":null,"abstract":"Rose is an important floricultural crop that has been exploited for many uses. Its important uses in different industries include, pharmaceutical, perfumery, and food industries that manifest higher flower yield. Therefore, response of Gruss-an-Taplitz to foliar application of chitosan (Ct) solution (0, 2.5, 5, 7.5, 10 mg L-1), was evaluated in an experimental field. Ct treatment had significant effects on studied parameters, including plant growth, pigments, enzymes, and gaseous exchange. This experiment was laid out under Randomized Complete Block Design (RCBD) using three replications per treatment. Ct (7.5 mg L-1) significantly improved growth, in terms of higher leaf area (20.37%), plant height (20.19%), number of flowers (55.51%), flower weight (34.64%) and flower diameter (33.78%) as well as enhancing relative water contents (27.38%) with respect to control. Chlorophyll a (54.60%), Chlorophyll b (12.13%), Carotenoid (8.36%) and anthocyanins (17.09%) were also increased at 7.5 mg L-1 Ct, which showed higher photosynthetic pigments as compared to control. Consequently, Ct (7.5 mg L-1) treated plants showed higher enzymatic activity; CAT (9.94%), SOD (83.87%), POD (64.54%), total antioxidant (35.48%), phenolics (7.41%) and gaseous exchange; Pn (55.65%), E (31.76%), and gs (18.38%) Ci (34.17%) that improved the plant growth and productivity of Gruss-an-Taplitz. Foliar application of 7.5 mg L-1 Ct improved biomass, water preservation, pigments, enzymatic activity and leaf gaseous exchange, resulted in higher production and quality of Gruss-an-Taplitz plants.","PeriodicalId":49933,"journal":{"name":"Kuwait Journal of Science & Engineering","volume":"301 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73372444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bader Al-Hamar, Anandavalli. R Inbamani, Saad A. Komona, M. Fouzi, R. Ben-Hamadou, H. Pereira, A. Bumajdad
Bioprospecting programmes are the key to increasing the current portfolio of indigenous microalgal strains accessible for different applications in microalgal biotechnology. In this work, nine fastgrowing microalgal strains isolated from the Arabian/Persian Gulf coastal waters of Kuwait were evaluated for their potential as biofuel feedstocks. 18S rRNA gene sequencing showed that the strains belong to five different genera, namely, Chlorella, Nannochloris, Scenedesmus, Tetraselmis, and Nannochloropsis. In terms of the total lipid content, in comparison to the other strains, Tetraselmis sp. KUBS13G and Tetraselmis sp. KUBS16G displayed higher lipid contents of 29.56% dry weight (DW) and 26.13% DW, respectively, which were dominated by palmitic and oleic acids. Fuel properties calculated from the fatty acid methyl esters (FAMEs) by empirical equations were compared with EN14214 (European) and ASTM D6751-−02 (American) biodiesel standards. Multicriteria decision analysis (MCDA) methods, such as the Preference Ranking Organization Method for Enrichment Evaluation (PROMETHEE) and Graphical Analysis for Interactive Assistance (GAIA), were used to select suitable microalgae for biofuel feedstock based on their biodiesel fuel properties. Overall, the results suggested that the indigenous microalgal strain Tetraselmis, in particular Tetraselmis sp. KUBS37G, and Scenedesmus sp. KUBS17R are the most suitable strains for biofuel feedstock owing to their improved fuel properties, such as density (ρ) (0.88 g cm−3), low kinematic viscosity (3.1 mm2 s−1), high cetane number (54 and 56, respectively), high oxidation stability (14.6 hr and 14.8 hr), and cold-filter plugging point (1.0°C and -6.1°C).
{"title":"Bioprospecting autochthonous marine microalgae strain from the Arabian Gulf Seawater, Kuwait for biofuel feedstocks","authors":"Bader Al-Hamar, Anandavalli. R Inbamani, Saad A. Komona, M. Fouzi, R. Ben-Hamadou, H. Pereira, A. Bumajdad","doi":"10.48129/kjs.11367","DOIUrl":"https://doi.org/10.48129/kjs.11367","url":null,"abstract":"Bioprospecting programmes are the key to increasing the current portfolio of indigenous microalgal strains accessible for different applications in microalgal biotechnology. In this work, nine fastgrowing microalgal strains isolated from the Arabian/Persian Gulf coastal waters of Kuwait were evaluated for their potential as biofuel feedstocks. 18S rRNA gene sequencing showed that the strains belong to five different genera, namely, Chlorella, Nannochloris, Scenedesmus, Tetraselmis, and Nannochloropsis. In terms of the total lipid content, in comparison to the other strains, Tetraselmis sp. KUBS13G and Tetraselmis sp. KUBS16G displayed higher lipid contents of 29.56% dry weight (DW) and 26.13% DW, respectively, which were dominated by palmitic and oleic acids. Fuel properties calculated from the fatty acid methyl esters (FAMEs) by empirical equations were compared with EN14214 (European) and ASTM D6751-−02 (American) biodiesel standards. Multicriteria decision analysis (MCDA) methods, such as the Preference Ranking Organization Method for Enrichment Evaluation (PROMETHEE) and Graphical Analysis for Interactive Assistance (GAIA), were used to select suitable microalgae for biofuel feedstock based on their biodiesel fuel properties. Overall, the results suggested that the indigenous microalgal strain Tetraselmis, in particular Tetraselmis sp. KUBS37G, and Scenedesmus sp. KUBS17R are the most suitable strains for biofuel feedstock owing to their improved fuel properties, such as density (ρ) (0.88 g cm−3), low kinematic viscosity (3.1 mm2 s−1), high cetane number (54 and 56, respectively), high oxidation stability (14.6 hr and 14.8 hr), and cold-filter plugging point (1.0°C and -6.1°C).","PeriodicalId":49933,"journal":{"name":"Kuwait Journal of Science & Engineering","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75497330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Baseerat Rumman, R. Habib, S. Awan, Mbah Ntepe Leonel Javeres, K. Khan, Khulah Sadia, Tekes Kornelia, S. Nurulain
Psychiatric disorders are complex mental conditions that cause significant emotional distress and impairment in a person’s ability to function normally. Globally, there is an alarming rise in the prevalence of psychiatric conditions. Genetic and environmental factors are involved in the pathophysiology of these disorders, but molecular underpinning is still elusive. Cholinergic dysregulation is one of the aetiology of psychiatric condition. This study was aimed to assess the status of hydrolyzing enzyme of cholinergic neurotransmitter, acetylcholinesterase (AChE) from blood and investigate the possible association of a single nucleotide polymorphisms in 3'UTR region of ACHE (rs17228602) with predisposition to psychiatric disorder. Methods: Ninety-five confirmed psychiatric and one hundred thirty healthy individuals were recruited for the study with due consents. AChE was determined by Elman’s method. SNP was studied by polymerase chain reaction-restricted fragment length polymorphism (PCR-RFLP) and sanger sequencing on DNA samples. Results: The results showed notably reduced AChE in psychiatric cohorts with statistical significance (p≤0.05). Genotype and allelic association of the examined SNP was observed with the risk of psychiatric condition in patients.
{"title":"Acetylcholinesterase and Association 1 of ACHE 3’ UTR SNP rs17228602 with Psychiatric Disorders","authors":"Baseerat Rumman, R. Habib, S. Awan, Mbah Ntepe Leonel Javeres, K. Khan, Khulah Sadia, Tekes Kornelia, S. Nurulain","doi":"10.48129/kjs.12465","DOIUrl":"https://doi.org/10.48129/kjs.12465","url":null,"abstract":"Psychiatric disorders are complex mental conditions that cause significant emotional distress and impairment in a person’s ability to function normally. Globally, there is an alarming rise in the prevalence of psychiatric conditions. Genetic and environmental factors are involved in the pathophysiology of these disorders, but molecular underpinning is still elusive. Cholinergic dysregulation is one of the aetiology of psychiatric condition. This study was aimed to assess the status of hydrolyzing enzyme of cholinergic neurotransmitter, acetylcholinesterase (AChE) from blood and investigate the possible association of a single nucleotide polymorphisms in 3'UTR region of ACHE (rs17228602) with predisposition to psychiatric disorder. Methods: Ninety-five confirmed psychiatric and one hundred thirty healthy individuals were recruited for the study with due consents. AChE was determined by Elman’s method. SNP was studied by polymerase chain reaction-restricted fragment length polymorphism (PCR-RFLP) and sanger sequencing on DNA samples. Results: The results showed notably reduced AChE in psychiatric cohorts with statistical significance (p≤0.05). Genotype and allelic association of the examined SNP was observed with the risk of psychiatric condition in patients.","PeriodicalId":49933,"journal":{"name":"Kuwait Journal of Science & Engineering","volume":"79 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86457143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Osibemhe, B. O. Orji, G. O. Omaji, E. Amune, J. Ezekiel
The relationship between diabetes mellitus and sex and/or thyroid hormones has been well documented in both human and animal studies ditto auto-reversibility of alloxan. However, the correlation between unstable diabetes and these hormones has little or no information in the literature; hence, the focus of this study. Diabetes was induced with a single intraperitoneal injection of 150 mg/kg of freshly prepared alloxan. Twenty five adult male Wistar rats (weight 120-150 g) were used in this study. Alloxan was administered to 20 rats and 5 rats served as control. Alloxan-administered rats were further divided into two groups. One group (diabetic rats) was used as diabetic control and the other group served as reversed diabetic, which contained rats whose fasting blood glucose was confirmed to be normoglycemic post-diabetic. All rats were maintained on normal rat feed and water ad libitum and were monitored for 14 days. Blood glucose was monitored at intervals of 7 days after basal (before diabetes induction) and day 1(diabetes confirmation) values had been noted. Sex hormones: Luteinizing hormone (LH), follicle stimulating hormone (FSH), and estrogen (E) as well as thyroid hormones: Triiodothyronine (T3), tetraiodothyronine (T4) and thyroid stimulating hormone (TSH) were assayed after 14 days. Alloxan caused alteration in blood glucose levels of both diabetic and reversed diabetic groups. T4 level was lowered significantly in both diabetic (11.32 ± 0.26 μg/ml) and reversed diabetic (11.00 ± 0.16 μg/ml) groups in relation to the control. Other assayed hormones were not different significantly from the control. These findings indicate that influence of diabetes on these hormones may not be dependent on glucose gradation.
{"title":"INSTABILITY OF ALLOXAN-INDUCED DIABETES AND ITS IMPACT ON SEX AND THYROID HORMONES IN MALE WISTAR RATS-A PILOT STUDY","authors":"M. Osibemhe, B. O. Orji, G. O. Omaji, E. Amune, J. Ezekiel","doi":"10.48129/kjs.15291","DOIUrl":"https://doi.org/10.48129/kjs.15291","url":null,"abstract":"The relationship between diabetes mellitus and sex and/or thyroid hormones has been well documented in both human and animal studies ditto auto-reversibility of alloxan. However, the correlation between unstable diabetes and these hormones has little or no information in the literature; hence, the focus of this study. Diabetes was induced with a single intraperitoneal injection of 150 mg/kg of freshly prepared alloxan. Twenty five adult male Wistar rats (weight 120-150 g) were used in this study. Alloxan was administered to 20 rats and 5 rats served as control. Alloxan-administered rats were further divided into two groups. One group (diabetic rats) was used as diabetic control and the other group served as reversed diabetic, which contained rats whose fasting blood glucose was confirmed to be normoglycemic post-diabetic. All rats were maintained on normal rat feed and water ad libitum and were monitored for 14 days. Blood glucose was monitored at intervals of 7 days after basal (before diabetes induction) and day 1(diabetes confirmation) values had been noted. Sex hormones: Luteinizing hormone (LH), follicle stimulating hormone (FSH), and estrogen (E) as well as thyroid hormones: Triiodothyronine (T3), tetraiodothyronine (T4) and thyroid stimulating hormone (TSH) were assayed after 14 days. Alloxan caused alteration in blood glucose levels of both diabetic and reversed diabetic groups. T4 level was lowered significantly in both diabetic (11.32 ± 0.26 μg/ml) and reversed diabetic (11.00 ± 0.16 μg/ml) groups in relation to the control. Other assayed hormones were not different significantly from the control. These findings indicate that influence of diabetes on these hormones may not be dependent on glucose gradation.","PeriodicalId":49933,"journal":{"name":"Kuwait Journal of Science & Engineering","volume":"10 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72449464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. Ahmad, A. Zehra, A. Jalal, Waqar Ali, Muhammad S. Khan
The genetic structure of salt-tolerant halophytic grass Sporobolus ioclados collected from inland and coastal areas of Pakistan was determined using P450 based analog (PBA); the functional genomic markers. Our data revealed high polymorphism (96%) in the collected germplasm. The high genetic variability could be attributed to outcrossing, and sexual reproduction. An analysis of molecular variance depicted higher genetic diversity (80%) within the populations and lower (20%) among the populations. The low genetic diversity among the inland and coastal populations might be due to fragmentation and long physical distances. The dendrogram and principal component analysis clearly distinguished the genotypes of the two populations into two distinct clusters. Our data demonstrated that PBA markers are reliable and reproducible for assessing genetic diversity in halophytic grass and provides valuable insight for future breeding and conservation programs.
{"title":"Determination of the genetic diversity in populations of halophytic grass S. ioclados using PBA markers","authors":"D. Ahmad, A. Zehra, A. Jalal, Waqar Ali, Muhammad S. Khan","doi":"10.48129/kjs.12893","DOIUrl":"https://doi.org/10.48129/kjs.12893","url":null,"abstract":"The genetic structure of salt-tolerant halophytic grass Sporobolus ioclados collected from inland and coastal areas of Pakistan was determined using P450 based analog (PBA); the functional genomic markers. Our data revealed high polymorphism (96%) in the collected germplasm. The high genetic variability could be attributed to outcrossing, and sexual reproduction. An analysis of molecular variance depicted higher genetic diversity (80%) within the populations and lower (20%) among the populations. The low genetic diversity among the inland and coastal populations might be due to fragmentation and long physical distances. The dendrogram and principal component analysis clearly distinguished the genotypes of the two populations into two distinct clusters. Our data demonstrated that PBA markers are reliable and reproducible for assessing genetic diversity in halophytic grass and provides valuable insight for future breeding and conservation programs.","PeriodicalId":49933,"journal":{"name":"Kuwait Journal of Science & Engineering","volume":"47 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78814692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Farooq Ali, Q. Shakeela, B. Uzma, Asma Bibi, Bushra Najeeb, A. U. Rahman, Mubassir Shah, Shehzad Ahmed
A multidrug-resistant bacterium due to its intrinsic resistance nature and beta-lactamases production, Pseudomonas aeruginosa can colonize ubiquitously and is thus associated with life-threating bacterial infections. The study was aimed to inspect phenotypic detection and antimicrobial resistance pattern of extended spectrum beta lactamase- and metallo beta lactamase-producing P. aeruginosa strains. Clinical specimens (n=220) were collected from indoor patients with ear discharge were inoculated on suitable culture media. Antimicrobial resistance pattern of all isolates was investigated employing Kerby-Bauer disc diffusion method. Double-disc synergy test and imipenem-EDTA test were used respectively to detect ESBL- and MBL-producing strains. From n=220, n=37 (16.82%) clinical specimens confirmed the growth of P. aeruginosa. In comparison to female (43.24%), male population (56.76%) was more prevalent. Out of n=37 positive cases, n=16 (43.24%) strains were detected as ESBL producers, while n=07 (18.92%) as MBL producers. Cefotaxime (100%) was found the most resisted antibiotic by isolates, followed by aztreonam (91.89%), gentamycin (86.49%), ceftriaxone (83.78%) and tazobactam/piperacillin (64.87%), while the lowest resistance was observed against imipenem (21.63%) and meropenem (51.36%). Furthermore, ESBLproducing strains revealed high resistance against cefotaxime (100%), ceftriaxone (93.75%), and carbenicillin (87.5%), while MBL-producing strains were completely resistance to imipenem, meropenem, cefotaxime and carbenicillin, followed by gentamicin (85.71%), amikacin aztreonam, ciprofloxacin and tazobactam/piperacillin (71.43% each). Our study concluded that strains of P. aeruginosa producing ESBL and MBL enzymes were mostly resistant to the drugs of choice, which puzzle the physicians to treat infections caused by P. aeruginosa. So, it is needed to study the resistant pattern of P. aeruginosa in order to recommend proper medication.
{"title":"Antimicrobial resistance pattern and phenotypic detection of Extended spectrum beta lactamase- and Metallo beta lactamase- producing Pseudomonas aeruginosa isolated from indoor-patients suffering ear discharge","authors":"Farooq Ali, Q. Shakeela, B. Uzma, Asma Bibi, Bushra Najeeb, A. U. Rahman, Mubassir Shah, Shehzad Ahmed","doi":"10.48129/kjs.10773","DOIUrl":"https://doi.org/10.48129/kjs.10773","url":null,"abstract":"A multidrug-resistant bacterium due to its intrinsic resistance nature and beta-lactamases production, Pseudomonas aeruginosa can colonize ubiquitously and is thus associated with life-threating bacterial infections. The study was aimed to inspect phenotypic detection and antimicrobial resistance pattern of extended spectrum beta lactamase- and metallo beta lactamase-producing P. aeruginosa strains. Clinical specimens (n=220) were collected from indoor patients with ear discharge were inoculated on suitable culture media. Antimicrobial resistance pattern of all isolates was investigated employing Kerby-Bauer disc diffusion method. Double-disc synergy test and imipenem-EDTA test were used respectively to detect ESBL- and MBL-producing strains. From n=220, n=37 (16.82%) clinical specimens confirmed the growth of P. aeruginosa. In comparison to female (43.24%), male population (56.76%) was more prevalent. Out of n=37 positive cases, n=16 (43.24%) strains were detected as ESBL producers, while n=07 (18.92%) as MBL producers. Cefotaxime (100%) was found the most resisted antibiotic by isolates, followed by aztreonam (91.89%), gentamycin (86.49%), ceftriaxone (83.78%) and tazobactam/piperacillin (64.87%), while the lowest resistance was observed against imipenem (21.63%) and meropenem (51.36%). Furthermore, ESBLproducing strains revealed high resistance against cefotaxime (100%), ceftriaxone (93.75%), and carbenicillin (87.5%), while MBL-producing strains were completely resistance to imipenem, meropenem, cefotaxime and carbenicillin, followed by gentamicin (85.71%), amikacin aztreonam, ciprofloxacin and tazobactam/piperacillin (71.43% each). Our study concluded that strains of P. aeruginosa producing ESBL and MBL enzymes were mostly resistant to the drugs of choice, which puzzle the physicians to treat infections caused by P. aeruginosa. So, it is needed to study the resistant pattern of P. aeruginosa in order to recommend proper medication.","PeriodicalId":49933,"journal":{"name":"Kuwait Journal of Science & Engineering","volume":"119 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89409914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A survey was carried out to diagnose the rates of mortality of lambs in the sheep farms of Kuwait. The survey results helped determine that the cause of high mortality rate in Kuwait of newborn lambs (≈35%) during the first three weeks of age is the certain infectious diseases affecting the sheep. Following the survey, a field experiment was carried out using a modified vaccination protocol (vaccination during pregnancy) to improve the immune status of the secreted colostrum. This new protocol has improved the immunity of newborn lambs after suckling the hyperimmune colostrum, and reduced mortality rate significantly, compared to the conventional protocol (vaccination before pregnancy). The results have proved the efficiency of the modified vaccination protocol. The experiment concluded that the vaccination during pregnancy has shown great promise to reduce the mortality rate of newborn lambs in sheep farms of Kuwait. Upscaling the tested vaccination protocol may set the scene for the betterment of sheep industry of Kuwait.
{"title":"Diagnosing and Mitigating the Risks of Lambs’ Mortality in the Sheep Farms of Kuwait","authors":"H. Burezq, Faten Khalil","doi":"10.48129/kjs.14607","DOIUrl":"https://doi.org/10.48129/kjs.14607","url":null,"abstract":"A survey was carried out to diagnose the rates of mortality of lambs in the sheep farms of Kuwait. The survey results helped determine that the cause of high mortality rate in Kuwait of newborn lambs (≈35%) during the first three weeks of age is the certain infectious diseases affecting the sheep. Following the survey, a field experiment was carried out using a modified vaccination protocol (vaccination during pregnancy) to improve the immune status of the secreted colostrum. This new protocol has improved the immunity of newborn lambs after suckling the hyperimmune colostrum, and reduced mortality rate significantly, compared to the conventional protocol (vaccination before pregnancy). The results have proved the efficiency of the modified vaccination protocol. The experiment concluded that the vaccination during pregnancy has shown great promise to reduce the mortality rate of newborn lambs in sheep farms of Kuwait. Upscaling the tested vaccination protocol may set the scene for the betterment of sheep industry of Kuwait.","PeriodicalId":49933,"journal":{"name":"Kuwait Journal of Science & Engineering","volume":"80 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78845946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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