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Eradication of Klebsiella pneumoniae biofilms and persister cells using silver nitrate 用硝酸银根除肺炎克雷伯菌生物膜和持久性细胞
Pub Date : 2022-05-10 DOI: 10.48129/kjs.15153
Sarah N. Aziz, M. Marjani
To perceive the relationship between the emergences of antibiotics persistence, biofilm formation, and to study the role of silver nitrate in fighting these microbes, a total 50 clinical Klebsiella pneumonae isolates were collected from clinical sources and identified using genotypic identification by using specific primer (rpoB gene) from housekeeping genes. Detection of their biofilm-forming abilities were carried out. The eradication effect of silver nitrate to control bacterial persistence and reducing the biofilm-forming abilities were studied. We found that 40 isolate (80%) were biofilm-formers. From bacterial persisters, (75%) were biofilm-formers. Silver nitrate showed a great role to eradicate persister cells and inhibition of bacterial biofilm formation of clinical isolates of K. pneumoniae.
为了了解抗生素持久性的出现与生物膜的形成之间的关系,并研究硝酸银在对抗这些微生物中的作用,我们从临床来源收集了50株临床肺炎克雷伯菌分离株,并使用来自内源基因的特异性引物(rpoB基因)进行了基因型鉴定。对其生物膜形成能力进行了检测。研究了硝酸银对控制细菌滞留和降低生物膜形成能力的根除效果。我们发现40株(80%)是生物成膜菌。从细菌持久性中,(75%)是生物膜形成者。硝酸银对临床分离的肺炎克雷伯菌具有杀灭持久性细胞和抑制细菌生物膜形成的作用。
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引用次数: 1
Local Site effects for ground motion acceleration and amplification at Bahrah, Saudi Arabia. 沙特阿拉伯Bahrah地震动加速和放大的局部场址效应。
Pub Date : 2022-04-02 DOI: 10.48129/kjs.12349
F. Rehman, A. Alamri, S. El-Hady, Hussein M. Harbi, M. F. Ullah, Saif-ur- Rehman
The soil conditions variability at site strongly influence ground motions during earthquakes. The disastrous earthquakes have shown that the local site conditions are the essential factors that impact lsurficial ground motions. In present study, local site effects are evaluated for ground motion acceleration and amplification at Bahrah. The town is situated on which is covered by quaternary alluvial wadi deposits. The ground motion time series are obtained through Spectral matching technique. is utilized to generate well matched ground motion time series based on the results of the PSHA and the deaggregation process. The ground motion time series and shallow Geophysical data are utilized to carry out One-dimensional simulation is carried out for equivalent linear seismic response analyses for soil layers. Results of One-dimensional site response analysis are interpolated to generate maps that account for ground motion accelerations and amplifications at top of the soil for selected spectral periods (PGA, 0.1, 0.2 and 2) for 475 years return period. The maps show the ground shaking rather than the probability of damage to buildings. The maximum values of amplification ranges upto 3.46 and acceleration upto 0.86 g. The undulation of bed rock topography and existence of deep soft sediments are reflected considerably on the results of the study area. The surface ground motion maps show that the seismic hazard level in Bahrah is moderate.
地震时现场土壤条件的变化对地面运动有很大的影响。灾害性地震表明,局部场地条件是影响地表运动的重要因素。本研究评估了Bahrah地区地震动加速和放大的局部场地效应。该镇位于被第四纪冲积河沉积物覆盖的地区。利用光谱匹配技术获得了地面运动时间序列。基于PSHA和解聚过程的结果生成匹配良好的地震动时间序列。利用地面运动时间序列和浅层地球物理资料进行一维模拟,对土层进行等效线性地震反应分析。将一维场地响应分析的结果内插到475年回归期的选定光谱周期(PGA、0.1、0.2和2)中,生成地表运动加速度和土壤顶部放大的地图。地图显示的是地面震动,而不是建筑物受损的可能性。放大的最大值可达3.46,加速度可达0.86 g。基岩地形起伏和深部软质沉积物的存在对研究区的结果有较大的反映。地面地动图显示,巴拉地区地震危险性为中等。
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引用次数: 0
Transparent Dielectric TiO2/SiO2 Coatings for Thermal Shielding and Self-Cleaning Applications 用于热屏蔽和自清洁应用的透明介质TiO2/SiO2涂层
Pub Date : 2022-04-02 DOI: 10.48129/kjs.17787
Venkatesh Yepuri, D. R.S.
The economical fabrication of one-dimensional dielectric reflectors using the hybrid sol-gel spin coating process is significant compared to sophisticated chemical and physical vapor deposition techniques. In this work, we opted for alternate layers of TiO2 and SiO2 films to fabricate near-infrared dielectric reflectors owing to their high refractive index contrast and easy tunability of the reflection window in the desired spectral range. X-ray diffraction (XRD) studies of the monolayer TiO2 and SiO2 confirmed the existence of anatase-TiO2 and amorphous-SiO2 phases, respectively. Spectroscopic ellipsometry investigation of TiO2 and SiO2 films revealed the refractive indices of 2.6 and 1.5, respectively. The field-emission scanning electron microscopy (FESEM) analyses evidenced the fabrication of 2.5 stacks/bilayers of TiO2/SiO2 (TiO2/SiO2)2.5S. The reflectance measurement demonstrated 100% reflection in the near-infrared region with its center wavelength of 833 nm. In addition, we have examined the water contact angle of various samples using the sessile drop technique, and 2.5 stacks-based reflector showed its lowest contact angle of 29.3o, which suggests its anti-fogging and self-cleaning applications.
与复杂的化学和物理气相沉积技术相比,采用溶胶-凝胶混合自旋镀膜工艺经济地制造一维介质反射器具有重要意义。在这项工作中,我们选择了TiO2和SiO2薄膜的交替层来制造近红外介质反射器,因为它们具有高折射率对比度和易于在所需光谱范围内调节反射窗口。对单层TiO2和SiO2的x射线衍射(XRD)研究分别证实了锐钛矿-TiO2和无定形-SiO2相的存在。对TiO2和SiO2薄膜的椭偏光谱研究表明,其折射率分别为2.6和1.5。场发射扫描电镜(FESEM)分析证实了2.5层/双层TiO2/SiO2 (TiO2/SiO2)2.5 s的制备。在中心波长为833 nm的近红外区域,反射率达到100%。此外,我们还利用固滴技术检测了各种样品的水接触角,2.5层叠式反射器的最低接触角为29.30,表明其具有防雾和自清洁的应用价值。
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引用次数: 1
Four dimensional matrix mappings and applications 四维矩阵映射及其应用
Pub Date : 2022-04-02 DOI: 10.48129/kjs.17649
Mehmet Ali Sarıg¨ol
In this paper, we characterize the classes (L,Lk) , (Lk,L) and (L∞,Lk) , 1 ≤ k < ∞, of all four dimensional infinite matrices, where Lk and L∞ are the spaces of all absolutely k-summable and bounded double sequences, respectively. Using them, we establish some relations between N, pn, qn and N, p′ n, q′n k summability methods which extend some results of Bosanquet (1950), Sarıg¨ol (1993), Sarıg¨ol and Bor (1995), and Sunouchi (1949) to double summability methods, and give a relation between single and double summability methods.
本文刻画了所有四维无限矩阵的类(L,Lk)、(Lk,L)和(L∞,Lk), 1≤k <∞,其中Lk和L∞分别是所有绝对k可和和有界二重序列的空间。利用它们,我们建立了N、pn、qn与N、p′N、q′k可和性方法之间的关系,将Bosanquet(1950)、Sarıg¨ol(1993)、Sarıg¨ol与Bor(1995)、Sunouchi(1949)的一些结果推广到双可和性方法,并给出了单可和性方法与双可和性方法之间的关系。
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引用次数: 2
Investigation of biomimetic hydroxyapatite formation on titania nanoparticles and lobed nanotubes 仿生羟基磷灰石在二氧化钛纳米颗粒和叶状纳米管上形成的研究
Pub Date : 2022-04-02 DOI: 10.48129/kjs.18605
Murthada Kutheir Abbas, M. S. Hashim
An anodizing method was used to produce TiO2 nanotubes (TNTs) on Ti metal bases. These tubes were transformed into lobed nanotubes (LNTs) by heating at 6000C as scanning electron microscopy (SEM) images showed. A rapid break down anodizing (RBA) technique was utilized to produce TiO2 nanoparticles (TNPs). These particles were deposited on LNTs by the electrophoretic deposition (EPD) method. An X-ray diffraction (XRD) test revealed the polycrystalline composition of the formed LNTs and the amorphous structure of TNPs. The bioactivity of LNTs and the deposited TNPs on the LNTs were tested by immersing them in simulated body fluid (SBF) for one month. For the two samples, XRD patterns showed the appearance of small peaks of hydroxyapatite (HAp), which indicates the bioactivity of these samples. SEM images show that the HAp layer on LNTs was in the form of protrusions, while on TNPs it was in the form of crossed filaments.
采用阳极氧化法制备了钛金属基上的TiO2纳米管。扫描电子显微镜(SEM)图像显示,这些管在6000℃加热后转化为叶状纳米管(LNTs)。采用快速分解阳极氧化(RBA)技术制备TiO2纳米颗粒(TNPs)。这些颗粒通过电泳沉积(EPD)方法沉积在LNTs上。x射线衍射(XRD)测试揭示了形成的LNTs的多晶组成和TNPs的非晶结构。通过模拟体液(SBF)浸泡1个月,检测LNTs及其上沉积TNPs的生物活性。两种样品的XRD谱图均显示出羟基磷灰石(HAp)的小峰,表明样品具有生物活性。扫描电镜显示,在LNTs上HAp层呈突起状,而在TNPs上HAp层呈交叉细丝状。
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引用次数: 1
Applications of well logging techniques to evaluate the groundwater aquifers in the area between southwest Bani Sweif and west Asyoute governorate, upper Egypt. 测井技术在上埃及Bani Sweif西南部和Asyoute省西部地区地下水含水层评价中的应用
Pub Date : 2022-04-02 DOI: 10.48129/kjs.17333
Safi Eldein.M. Metwally, Shimaa. M. Elska, Fardous M. Zarif, Abdulla. F. Saad
The relevance of detecting aquifer characterization and aquifer potential has risen with the application of well logging techniques as the demand for water has increased. Apart from pumping data, 16 geophysical well logs (resistivity, gamma ray, self-potential, and nuclear logs) are utilized to achieve the main goal of estimating petrophysical parameters (porosity (∅), effective porosity (∅𝑒𝑓𝑓), hydraulic conductivity (k), permeability (K) and shale volume (𝑉𝑠ℎ)) of the Apollonia (Eocene fractured limestone aquifer), Upper and Lower Bahariya (Nubian sandstone aquifer) formations. The findings show that the Eocene fractured limestone aquifer has a carbonate in soft chalk with high K and ∅ as well as high k of 4974.39 mD at well ST-188 (a highly productive aquifer), whereas the Lower Bahariya formation is mostly fine to medium-grained sandstone and clay, suggesting that it formed in shallow marine environments with low K. On the other hand, Upper Bahariya was noted that having poor sand succession. As results shown, K of the most examined wells ranges from 2609.69 to 1486.812 mD at ST-258 and ST-38 wells respectively. The study appears that 𝑉𝑠ℎ, along with ∅ and K features, is regarded as an important attribute in aquifer evaluation where, estimated 𝑉𝑠ℎ over the studied area is ranges between 5.96% and 31.7% at ST-48 and WBS1 wells respectively. Overall, this study provides an important insight into the importance of evaluating groundwater aquifers over time to help in making decisions to save and protect groundwater aquifers in the future.
随着对水需求的增加,测井技术的应用提高了探测含水层特征和含水层潜力的相关性。除泵送资料外,还利用16口地球物理测井(电阻率、伽马、自电位、核测井)实现了对Apollonia(初新世裂缝性灰岩含水层)、Bahariya上、下(努比亚砂岩含水层)地层岩石物性参数(孔隙度(∅)、有效孔隙度(∅𝑒𝑓𝑓)、导水率(k)、渗透率(k)、页岩体积(𝑠)的估算。结果表明:始新统裂缝灰岩含水层以高K、∅的软白垩质碳酸盐岩为主,ST-188井高K为4974.39 mD(高产含水层),而下Bahariya组多为细粒至中粒砂岩和粘土,形成于低K的浅海环境;上Bahariya组砂层演替较差。结果表明,ST-258井和ST-38井的K值分别为2609.69 ~ 1486.812 mD。研究表明,ST-48井和WBS1井研究区域的估算值分别为5.96% ~ 31.7%,因此,在含水层评价中,以∅和K为特征的∑𝑠是一个重要的属性。总的来说,这项研究提供了一个重要的见解,即随着时间的推移评估地下水含水层的重要性,有助于在未来做出拯救和保护地下水含水层的决定。
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引用次数: 0
QadirVax-19: A Multi Epitope-Based Vaccine against COVID-19 QadirVax-19:一种基于多表位的COVID-19疫苗
Pub Date : 2022-04-02 DOI: 10.48129/kjs.17575
Misha Mukhtar, M. Qadir
An outbreak of COVID-19, caused by a novel virus named SARS-corona virus 2 (SARS-CoV-2), has become a global challenge which needs to be addressed immediately. It has high rate of transmission and severity of the disease varying from person to person. Researchers are trying to find effective vaccines and therapeutic targets to control this novel type of coronavirus. In the present study, surface glycoprotein was used to identify B cell and T cell epitopes that have strong immunogenic potential. Highly conserved region of the surface glycoprotein was identified from seven related human coronaviruses. Epitopes and their prominent features were predicted by using immunoinformatics tools. Epitopes which have high binding energy were joined together through linker to form an epitope-based subunit vaccine construct. Molecular docking was performed by MOE to predict the binding energies of construct with B cell receptor, MHC Class I and MHC Class II receptors. The sequence of the multi-epitope construct finally came out as LQYGSFCTQLNRGPGPGTFGAGAALQGPGPGNFTTAPAICGPGPGHWFVTQRNFAAYQYIKWPWYI. It was named as QadirVax-19. Multi-epitope construct was highly antigenic along with proteasomal cleavage sites and full population coverage. The highest binding energies were obtained which shows that the construct has the ability to produce stronger humoral and immune response against structural glycoproteins of SARS-CoV-2. In-silico cloning of the construct revealed its stable expression in E. coli. Our study suggests that reverse vaccinology approaches give the immunogenic profile of the epitopes which helped us in designing the subunit vaccine against the SARS-corona virus 2.
由新型病毒sars -冠状病毒2 (SARS-CoV-2)引起的COVID-19疫情已成为一项需要立即应对的全球性挑战。它具有很高的传播率,疾病的严重程度因人而异。研究人员正在努力寻找有效的疫苗和治疗靶点来控制这种新型冠状病毒。在本研究中,表面糖蛋白被用来鉴定具有强免疫原性的B细胞和T细胞表位。从7种相关的人冠状病毒中鉴定出表面糖蛋白高度保守区。利用免疫信息学工具预测表位及其显著特征。将具有高结合能的表位通过连接体连接在一起,形成基于表位的亚单位疫苗结构。通过MOE进行分子对接,预测构建物与B细胞受体、MHC I类和MHC II类受体的结合能。最终得到的多表位构建序列为LQYGSFCTQLNRGPGPGTFGAGAALQGPGPGNFTTAPAICGPGPGHWFVTQRNFAAYQYIKWPWYI。它被命名为QadirVax-19。多表位结构具有高抗原性,具有蛋白酶体切割位点和全群体覆盖。获得了最高的结合能,表明该构建体能够对SARS-CoV-2的结构糖蛋白产生更强的体液和免疫应答。结果表明,该构建体在大肠杆菌中稳定表达。我们的研究表明,反向疫苗学方法提供了抗原表位的免疫原性特征,这有助于我们设计针对sars冠状病毒2的亚单位疫苗。
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引用次数: 0
Nutritional composition and biochemical characteristics of five date palm fruit (Phoenix dactylifera L.) varieties at the Khalal stage grown in Kuwait. 科威特五种枣椰果(Phoenix dactylifera L.)品种Khalal期营养成分及生化特性研究。
Pub Date : 2022-04-02 DOI: 10.48129/kjs.17169
M. Al-Hilal, Zeinab Moussa, A. Anderson
Palm dates are among the most highly cultivated and consumed fruits in Kuwait and other Arabian Gulf countries. However, data on the nutritional content of Kuwaiti date fruits remain scarce. The purpose of the current study was to determine the nutritional composition and biochemical characteristics of five date palm fruit varieties at the Khalal stage grown in Kuwait. Samples of five different cultivars (Berhi (samples from 2 different locations), Ikhlas, Saamaran, and Khanizi) grown in Kuwait were analyzed for energy, protein, carbohydrates, sugars, antioxidants, and micro-minerals. Varieties studied had high energy values ranging between 351.63 kcal/100g and 368.35 kcal/100g. They were rich in potassium (4450-7128mg/100g), with significant amounts of calcium (287.8-469.1mg/100g), magnesium (130.3 -294.5 mg/100g), sodium (70.7-123.3 mg/100g), iron (2.45-3.2 mg/100g) and manganese (0.7-1.55 mg/100g). Gallic (19.97-5.47 mg/100g), chlorogenic (0-0.712mg/100 g), and ascorbic (0.624-0.875 g/1mg) acids were the main antioxidants. The data indicate that the Kuwaiti date fruit is a rich source of essential nutrients making it an important local product that requires further investigations for its nutritional properties and industrial utilization prospects.
棕榈枣是科威特和其他阿拉伯海湾国家种植和消费最多的水果之一。然而,关于科威特枣果实营养成分的数据仍然很少。本研究的目的是确定在科威特种植的五种Khalal期椰枣果实品种的营养成分和生化特性。分析了科威特生长的5个不同品种(Berhi(来自2个不同地点的样品)、Ikhlas、Saamaran和Khanizi)的能量、蛋白质、碳水化合物、糖、抗氧化剂和微量矿物质。所研究品种的高能值在351.63 kcal/100g至368.35 kcal/100g之间。它们富含钾(4450-7128mg/100g)、钙(287.8-469.1mg/100g)、镁(130.3 -294.5 mg/100g)、钠(70.7-123.3 mg/100g)、铁(2.45-3.2 mg/100g)和锰(0.7-1.55 mg/100g)。没食子酸(19.97 ~ 5.47 mg/100g)、绿原酸(0 ~ 0.712mg/ 100g)和抗坏血酸(0.624 ~ 0.875g/1mg)是主要的抗氧化剂。数据表明,科威特枣果实是必需营养素的丰富来源,使其成为一种重要的当地产品,其营养特性和工业利用前景需要进一步研究。
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引用次数: 0
Comparison of fast regression algorithms in large datasets 大型数据集快速回归算法的比较
Pub Date : 2022-04-02 DOI: 10.48129/kjs.16159
S. Cangur, H. Ankarali
The aim is to compare the performances of fast regression methods, namely dimensional reduction of correlation matrix (DRCM), nonparametric dimensional reduction of correlation matrix (N-DRCM), variance inflation factor (VIF) regression, and robust VIF (R-VIF) regression in the presence of multicollinearity and outliers problems. In all simulation-scenarios, all the target variables were chosen for final models using four methods. The DRCM and N-DRCM are the methods that reach the final model in the shortest time, respectively. The time to reach the final model using R-VIF regression was approximately twice shorter than that of VIF regression. In each method, as the number of variables and the level of outliers increased, the time taken to reach the final model increased. When the level of multicollinearity and the number of variables (p > 500) increased, the times to reach the final models using DRCM in datasets with outliers were slightly shorter than the those of N-DRCM. The largest numbers of noise variables were selected to the model using DRCM and N-DRCM, but the least number of them were selected to the model using the R-VIF regression. The RMSE values obtained using DRCM, N-DRCM and VIF regression were similar in each scenario. As a result of the real dataset, the final model selected using R-VIF regression had the highest R2. It also had the lowest RMSE value among those obtained with other approaches excluding VIF regression. As such, the R-VIF regression method demonstrated a better performance than the others in all datasets.
目的是比较快速回归方法,即相关矩阵降维(DRCM)、非参数相关矩阵降维(N-DRCM)、方差膨胀因子(VIF)回归和鲁棒VIF (R-VIF)回归在多重共线性和离群值问题下的性能。在所有模拟场景中,使用四种方法选择所有目标变量作为最终模型。DRCM和N-DRCM分别是在最短时间内达到最终模型的方法。使用R-VIF回归得到最终模型的时间大约比使用VIF回归的时间短两倍。在每种方法中,随着变量数量和异常值水平的增加,到达最终模型所需的时间增加。当多重共线性水平和变量数量(p > 500)增加时,在有异常值的数据集上使用DRCM达到最终模型的时间略短于N-DRCM。采用DRCM和N-DRCM模型选择的噪声变量数量最多,而采用R-VIF回归模型选择的噪声变量数量最少。在每种情况下,采用DRCM、N-DRCM和VIF回归得到的RMSE值相似。由于真实数据集,使用R-VIF回归选择的最终模型R2最高。在除VIF回归外的其他方法中,它的RMSE值也最低。因此,R-VIF回归方法在所有数据集上都表现出比其他方法更好的性能。
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引用次数: 0
Sol-gel Synthesis of CaZnAl2O4 Ceramic Nanoparticles and Investigation of their Properties 溶胶-凝胶法制备纳米CaZnAl2O4陶瓷及其性能研究
Pub Date : 2022-04-02 DOI: 10.48129/kjs.17809
Sekhar Didde, Dubey R. S., S. Panda
Dielectric ceramic materials are well-recognized in the semiconductor industry because of their exceptional thermal stability, chemical resistance, and crystallinity. Despite their potential applications, these are also demanded in wireless communication. This paper reports the structural, morphological, and dielectric properties of sol-gel-derived CaZnAl2O4 ceramic nanoparticles. X-ray diffraction (XRD) analysis exhibited the polycrystalline characteristic of the CaZnAl2O4 nanoparticles with their crystallite size of 13 nm. Fourier-Transform infrared spectroscopy (FTIR) analysis confirmed the relevant vibration peaks of various functional groups present in the ceramic nanoparticles. Surface morphology study demonstrated the preparation of spherical grains with their mean diameter of 16 nm. The concentric rings also confirm the crystallinity of the nanoparticles that appeared in the selected area diffraction pattern. Furthermore, dielectric properties investigation showed the variation of dielectric permittivity from 23.76 to 21.67 as a function of increased frequency. Similarly, the dielectric loss is found to decrease from 0.047 to 0.039. As a result, the conductivity increased from 1.324 μS/m to 3.639 μS/m as a function of applied frequency.
介电陶瓷材料因其优异的热稳定性、耐化学性和结晶性而在半导体工业中得到广泛认可。尽管它们有潜在的应用,但在无线通信中也需要它们。本文报道了溶胶-凝胶法制备的CaZnAl2O4纳米陶瓷的结构、形态和介电性能。x射线衍射(XRD)分析表明,纳米CaZnAl2O4具有多晶特征,晶粒尺寸为13 nm。傅里叶变换红外光谱(FTIR)分析证实了陶瓷纳米颗粒中存在的各种官能团的相关振动峰。表面形貌研究表明制备出平均直径为16 nm的球形颗粒。同心圆也证实了出现在选定区域衍射图中的纳米颗粒的结晶度。此外,介电常数随频率的增加在23.76 ~ 21.67之间变化。同样,介质损耗从0.047降低到0.039。随着施加频率的增加,电导率从1.324 μS/m增加到3.639 μS/m。
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引用次数: 0
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Kuwait Journal of Science & Engineering
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