A. Krisnawati, A. Soegianto, Budi Waluyo, K. Kuswanto
The study is aimed at evaluating the pod shattering resistance of F8 soybean lines based on the shattering incidence and shattering severity. The materials consist of fourteen F8 soybean lines and two check cultivars. The pod shattering incidence was examined by using the oven-dry method, meanwhile, the shattering severity was evaluated based on the severity of the pod opening. The pod shattering resistance based on the shattering incidence resulted in five resistant lines (7–10% shattering), seven moderate lines (13–23% shattering), one susceptible line (53% shattering), and one very susceptible line (100% shattering). The pod shattering resistance based on the shattering severity showed that the pod opening on the ventral side differed between the lines and between the shattering degree, and it tends to form sigmoid curves with a different peak position for each shattering degree. The shattering severity of the resistant, moderate, and susceptible lines reached a peak at 60 °C, 50 °C, and 40 °C, respectively. A longer pod length indicated by the length of the dorsal (r = 0.827**) and ventral (r = 0.880**) sides of the pod, a higher total pod weight (0.827**), and a larger seed size (0.794**) will increase the degree of susceptibility to pod shattering. Those characteristics were considered to be the ones that should be used as the selection criteria in the breeding programme for pod shattering resistance in soybeans.
{"title":"The pod shattering resistance of soybean lines based on the shattering incidence and severity","authors":"A. Krisnawati, A. Soegianto, Budi Waluyo, K. Kuswanto","doi":"10.17221/20/2020-cjgpb","DOIUrl":"https://doi.org/10.17221/20/2020-cjgpb","url":null,"abstract":"The study is aimed at evaluating the pod shattering resistance of F8 soybean lines based on the shattering incidence and shattering severity. The materials consist of fourteen F8 soybean lines and two check cultivars. The pod shattering incidence was examined by using the oven-dry method, meanwhile, the shattering severity was evaluated based on the severity of the pod opening. The pod shattering resistance based on the shattering incidence resulted in five resistant lines (7–10% shattering), seven moderate lines (13–23% shattering), one susceptible line (53% shattering), and one very susceptible line (100% shattering). The pod shattering resistance based on the shattering severity showed that the pod opening on the ventral side differed between the lines and between the shattering degree, and it tends to form sigmoid curves with a different peak position for each shattering degree. The shattering severity of the resistant, moderate, and susceptible lines reached a peak at 60 °C, 50 °C, and 40 °C, respectively. A longer pod length indicated by the length of the dorsal (r = 0.827**) and ventral (r = 0.880**) sides of the pod, a higher total pod weight (0.827**), and a larger seed size (0.794**) will increase the degree of susceptibility to pod shattering. Those characteristics were considered to be the ones that should be used as the selection criteria in the breeding programme for pod shattering resistance in soybeans.","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":"56 1","pages":"111-122"},"PeriodicalIF":0.9,"publicationDate":"2020-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.17221/20/2020-cjgpb","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44532117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Assadollah Zakeri Haddadan, M. Ghaffari, E. Hervan, B. Alizadeh
To study the impact of parent-inbred lines on the heterosis expression of the agronomic characteristics in sunflower hybrids, 24 sunflower hybrids along with the parent lines were evaluated for their agronomic characteristics as a randomised complete block design with three replications in the 2018–2019 growing seasons in Karaj, Iran. According to the results, the hybrids R29 × A346, R19 × A346, R29 × A40 had the highest achene yield (4 159, 4 143 and 4 108 kg/ha, respectively), but the highest heterosis was observed in R29 × A212 and R19 × A212 (182 and 181%, respectively) suggesting that the incidence of heterosis is related to the relative performance of both the parents and hybrids. The results confirmed the heterosis expression for most of the agronomic traits. The heterosis for the days to flowering and maturity were negative. All the mid-parent heterosis (MPH) for the plant height, head diameter, stem diameter and achene number were positive, while only the plant height was positive for the best parent heterosis (BPH). Almost all the MPH and BPH of the crosses for the achene and oil yield were positive, which indicates a considerable heterosis for the achene and oil yield. The results showed that the relative impact of the restorer (R)-lines was higher than the cytoplasmic mail sterile (CMS)-lines on the heterosis expression for the days to maturity, stem diameter, achene number per head and achene and oil yield. The CMS-lines had more of an impact on the heterosis expression for the plant height and the relative impact of the R-lines and CMS-lines were almost similar for the days to flowering, head diameter, achene weight and oil content. Due to the higher relative impact of the paternal lines on the heterosis expression for half of the studied characteristics in this study, choosing suitable parental lines will have a crucial role in breeding the sunflowers for a desired trait.
{"title":"Impact of parent inbred lines on heterosis expression for agronomic characteristics in sunflower","authors":"Assadollah Zakeri Haddadan, M. Ghaffari, E. Hervan, B. Alizadeh","doi":"10.17221/100/2019-cjgpb","DOIUrl":"https://doi.org/10.17221/100/2019-cjgpb","url":null,"abstract":"To study the impact of parent-inbred lines on the heterosis expression of the agronomic characteristics in sunflower hybrids, 24 sunflower hybrids along with the parent lines were evaluated for their agronomic characteristics as a randomised complete block design with three replications in the 2018–2019 growing seasons in Karaj, Iran. According to the results, the hybrids R29 × A346, R19 × A346, R29 × A40 had the highest achene yield (4 159, 4 143 and 4 108 kg/ha, respectively), but the highest heterosis was observed in R29 × A212 and R19 × A212 (182 and 181%, respectively) suggesting that the incidence of heterosis is related to the relative performance of both the parents and hybrids. The results confirmed the heterosis expression for most of the agronomic traits. The heterosis for the days to flowering and maturity were negative. All the mid-parent heterosis (MPH) for the plant height, head diameter, stem diameter and achene number were positive, while only the plant height was positive for the best parent heterosis (BPH). Almost all the MPH and BPH of the crosses for the achene and oil yield were positive, which indicates a considerable heterosis for the achene and oil yield. The results showed that the relative impact of the restorer (R)-lines was higher than the cytoplasmic mail sterile (CMS)-lines on the heterosis expression for the days to maturity, stem diameter, achene number per head and achene and oil yield. The CMS-lines had more of an impact on the heterosis expression for the plant height and the relative impact of the R-lines and CMS-lines were almost similar for the days to flowering, head diameter, achene weight and oil content. Due to the higher relative impact of the paternal lines on the heterosis expression for half of the studied characteristics in this study, choosing suitable parental lines will have a crucial role in breeding the sunflowers for a desired trait.","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":"56 1","pages":"123-132"},"PeriodicalIF":0.9,"publicationDate":"2020-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.17221/100/2019-cjgpb","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44810337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Veronika Kselíková, T. Vyhnánek, P. Hanáček, P. Martínek
Grain hardness is an important quality trait of cereals. In the present work, the particle size index (PSI) was used to assess the variability in grain hardness of thirteen triticale genotypes, three wheat genotypes and one rye genotype grown in two subsequent seasons. The PSI values of the triticale samples ranged between 7.7 to 19.2%, representing a medium hard to a very hard grain. Moreover, the sequencing of genes in the Hardness (Ha) locus revealed a limited genetic variability in the grain hardness genes, namely secaloindoline-a (Sina) and secaloindoline-b (Sinb). Two possible new allelic variants were identified, one for each of the secaloindoline genes. The existing variability in the grain hardness as well as polymorphisms in its candidate genes are a good starting point for efforts in breeding new varieties of triticale with improved grain hardness.
{"title":"Grain hardness in triticale: a physical and molecular evaluation","authors":"Veronika Kselíková, T. Vyhnánek, P. Hanáček, P. Martínek","doi":"10.17221/96/2019-cjgpb","DOIUrl":"https://doi.org/10.17221/96/2019-cjgpb","url":null,"abstract":"Grain hardness is an important quality trait of cereals. In the present work, the particle size index (PSI) was used to assess the variability in grain hardness of thirteen triticale genotypes, three wheat genotypes and one rye genotype grown in two subsequent seasons. The PSI values of the triticale samples ranged between 7.7 to 19.2%, representing a medium hard to a very hard grain. Moreover, the sequencing of genes in the Hardness (Ha) locus revealed a limited genetic variability in the grain hardness genes, namely secaloindoline-a (Sina) and secaloindoline-b (Sinb). Two possible new allelic variants were identified, one for each of the secaloindoline genes. The existing variability in the grain hardness as well as polymorphisms in its candidate genes are a good starting point for efforts in breeding new varieties of triticale with improved grain hardness.","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":"56 1","pages":"102-110"},"PeriodicalIF":0.9,"publicationDate":"2020-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.17221/96/2019-cjgpb","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48210001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lan Bo, Ying-Qing Yang, S. Qiang, Hongfan Chen, Chen Jian, Yonghui Huang, G. Huo, Xiangmin Li
To understand the cause of loss of rice blast resistance, we studied the pathogenicity of Magnaporthe oryzae strains isolated from rice hybrid Wuyou 308 and evaluated its resistance genes. A total of 62 M. oryzae strains were isolated and tested in 7 Chinese rice varieties with varying degrees of resistance to rice blast and 30 blast-resistant monogenic lines. Fourteen physiological races of M. oryzae were identified: 8.55% belonging to the ZA group, 86.67% to the ZB group, and 5.00% to the ZC group. ZB15 was the most abundant race (45.00%). Five resistance genes, Pi-3(1), Pi-z5, Pi-k, Pi-kp(C), and Pi-k(C), conferred good resistance to the 62 strains, with resistance frequencies of 95.56, 91.11, 88.89, 82.22, and 82.22%, respectively. In contrast, Pi-a(2) had a resistance frequency of 0%. The hybrid combination Wuyou 308 was found to carry Pi-ta and Pi-b genes. Because Pi-ta and Pi-b both showed low resistance frequencies to M. oryzae isolated from Jiangxi, the hybrid rice variety Wuyou 308 could be infected by most of the 62 M. oryzae strains. The emergence and spread of rice blast disease in Wuyou 308 may thus be difficult to avoid when climatic conditions are favourable.
{"title":"Study of pathogenicity and genetic diversity of Magnaporthe oryzae isolated from rice hybrid Wuyou 308 and detection of resistance genes","authors":"Lan Bo, Ying-Qing Yang, S. Qiang, Hongfan Chen, Chen Jian, Yonghui Huang, G. Huo, Xiangmin Li","doi":"10.17221/64/2019-cjgpb","DOIUrl":"https://doi.org/10.17221/64/2019-cjgpb","url":null,"abstract":"To understand the cause of loss of rice blast resistance, we studied the pathogenicity of Magnaporthe oryzae strains isolated from rice hybrid Wuyou 308 and evaluated its resistance genes. A total of 62 M. oryzae strains were isolated and tested in 7 Chinese rice varieties with varying degrees of resistance to rice blast and 30 blast-resistant monogenic lines. Fourteen physiological races of M. oryzae were identified: 8.55% belonging to the ZA group, 86.67% to the ZB group, and 5.00% to the ZC group. ZB15 was the most abundant race (45.00%). Five resistance genes, Pi-3(1), Pi-z5, Pi-k, Pi-kp(C), and Pi-k(C), conferred good resistance to the 62 strains, with resistance frequencies of 95.56, 91.11, 88.89, 82.22, and 82.22%, respectively. In contrast, Pi-a(2) had a resistance frequency of 0%. The hybrid combination Wuyou 308 was found to carry Pi-ta and Pi-b genes. Because Pi-ta and Pi-b both showed low resistance frequencies to M. oryzae isolated from Jiangxi, the hybrid rice variety Wuyou 308 could be infected by most of the 62 M. oryzae strains. The emergence and spread of rice blast disease in Wuyou 308 may thus be difficult to avoid when climatic conditions are favourable.","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":"56 1","pages":"93-101"},"PeriodicalIF":0.9,"publicationDate":"2020-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.17221/64/2019-cjgpb","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41704228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Congcong Yang, Jian Ma, Cong Li, Min-jie Sun, Yaya Zou, Ting Li, Y. Mu, H. Tang, X. Lan
Thousand-grain weight (TGW) is an important trait affecting wheat production. We previously identified a major quantitative trait loci (QTL) controlling the TGW on the 2D chromosome of wheat using a recombinant inbred line (RIL) population constructed by the cross between Tibetan semi-wild wheat Q1028 (Q1028) and Zhengmai 9023 (ZM9023). The positive allele at this QTL is from ZM9023. To further characterise this QTL, here, we try to develop and validate the high-resolution melting (HRM) and sequence-characterised amplified region (SCAR) markers. One HRM marker (0C98-411) and two SCAR markers (E301-700 and B0BB-10470) were developed and integrated into the genetic map. All of these three markers were validated in three populations with different genetic backgrounds. 0C98-411 is the most closely linked marker that could trace QTgw.sau-2D in molecular marker assisted breeding.
{"title":"The development and validation of new DNA markers linked to the thousand-grain weight QTL in bread wheat (Triticum aestivum L.)","authors":"Congcong Yang, Jian Ma, Cong Li, Min-jie Sun, Yaya Zou, Ting Li, Y. Mu, H. Tang, X. Lan","doi":"10.17221/35/2019-cjgpb","DOIUrl":"https://doi.org/10.17221/35/2019-cjgpb","url":null,"abstract":"Thousand-grain weight (TGW) is an important trait affecting wheat production. We previously identified a major quantitative trait loci (QTL) controlling the TGW on the 2D chromosome of wheat using a recombinant inbred line (RIL) population constructed by the cross between Tibetan semi-wild wheat Q1028 (Q1028) and Zhengmai 9023 (ZM9023). The positive allele at this QTL is from ZM9023. To further characterise this QTL, here, we try to develop and validate the high-resolution melting (HRM) and sequence-characterised amplified region (SCAR) markers. One HRM marker (0C98-411) and two SCAR markers (E301-700 and B0BB-10470) were developed and integrated into the genetic map. All of these three markers were validated in three populations with different genetic backgrounds. 0C98-411 is the most closely linked marker that could trace QTgw.sau-2D in molecular marker assisted breeding.","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2020-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.17221/35/2019-cjgpb","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42348751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The occurrence of floral petal spots is common among flowering plants and plays a major role in attracting pollinators. Cotton genotypes having large red petal spots are frequent in the United States Department of Agriculture, National Plant Germplasm System Gossypium arboreum (L.) collection. One accession, PI 408798, showed a unique faint red petal spot phenotype. To genetically characterise this trait, a F2 population of 226 plants was developed by crossing PI 408798 with G. arboreum accession PI 529714 that lacked pigmented petal spots. From the population, 161 plants showed the presence of faint red petal spots; whereas, 65 plants lacked pigmented petal spots. These data supported the single dominant gene model for the presence of floral petal spots. Accession PI 408798 will provide an important genetic resource to characterise the genes involved in the pathway controlling floral pigmentation.
{"title":"Genetic characterisation of the petal spot phenotype for Gossypium arboreum accession PI 408798","authors":"J. Erpelding","doi":"10.17221/88/2019-cjgpb","DOIUrl":"https://doi.org/10.17221/88/2019-cjgpb","url":null,"abstract":"The occurrence of floral petal spots is common among flowering plants and plays a major role in attracting pollinators. Cotton genotypes having large red petal spots are frequent in the United States Department of Agriculture, National Plant Germplasm System Gossypium arboreum (L.) collection. One accession, PI 408798, showed a unique faint red petal spot phenotype. To genetically characterise this trait, a F2 population of 226 plants was developed by crossing PI 408798 with G. arboreum accession PI 529714 that lacked pigmented petal spots. From the population, 161 plants showed the presence of faint red petal spots; whereas, 65 plants lacked pigmented petal spots. These data supported the single dominant gene model for the presence of floral petal spots. Accession PI 408798 will provide an important genetic resource to characterise the genes involved in the pathway controlling floral pigmentation.","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":"56 1","pages":"79-83"},"PeriodicalIF":0.9,"publicationDate":"2020-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.17221/88/2019-cjgpb","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45072651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. A. Razak, N. Azman, R. Kamaruzaman, S. A. Saidon, Muhammad Fairuz Mohd Yusof, Siti Norhayati Ismail, M. A. Jaafar, Norziha Abdullah
Understanding genetic diversity is a main key for crop improvement and genetic resource management. In this study, we aim to evaluate the genetic diversity of the released Malaysian rice varieties using single nucleotide polymorphism (SNP) markers. A total of 46 released Malaysian rice varieties were genotyped using 1536 SNP markers to evaluate their diversity. Out of 1536 SNPs, only 932 SNPs (60.7%) represented high quality alleles, whereas the remainder either failed to amplify or had low call rates across the samples. Analysis of the 932 SNPs revealed that a total of 16 SNPs were monomorphic. The analysis of the SNPs per chromosome revealed that the average of the polymorphic information content (PIC) value ranged from 0.173 for chromosome 12 to 0.259 for chromosome 11, with an average of 0.213 per locus. The genetic analysis of the 46 released Malaysian rice varieties using an unweighted pair group method with arithmetic mean (UPGMA) dendrogram revealed the presence of two major groups. The analysis was supported by the findings from the STRUCTURE analysis which indicated the ∆K value to be at the highest peak at K = 2, followed by K = 4. The pairwise genetic distance of the shared alleles showed that the value ranged from 0.000 (MR159 MR167) to 0.723 (MRIA Setanjung), which suggested that MR159 and MR167 were identical, and that the highest dissimilarity was detected between MRIA 1 and Setanjung. The results of the study will be very useful for the variety identification, the proper management and conservation of the genetic resources, and the exploitation and utilisation in future breeding programmes.
{"title":"Genetic diversity of released Malaysian rice varieties based on single nucleotide polymorphism markers","authors":"S. A. Razak, N. Azman, R. Kamaruzaman, S. A. Saidon, Muhammad Fairuz Mohd Yusof, Siti Norhayati Ismail, M. A. Jaafar, Norziha Abdullah","doi":"10.17221/58/2019-cjgpb","DOIUrl":"https://doi.org/10.17221/58/2019-cjgpb","url":null,"abstract":"Understanding genetic diversity is a main key for crop improvement and genetic resource management. In this study, we aim to evaluate the genetic diversity of the released Malaysian rice varieties using single nucleotide polymorphism (SNP) markers. A total of 46 released Malaysian rice varieties were genotyped using 1536 SNP markers to evaluate their diversity. Out of 1536 SNPs, only 932 SNPs (60.7%) represented high quality alleles, whereas the remainder either failed to amplify or had low call rates across the samples. Analysis of the 932 SNPs revealed that a total of 16 SNPs were monomorphic. The analysis of the SNPs per chromosome revealed that the average of the polymorphic information content (PIC) value ranged from 0.173 for chromosome 12 to 0.259 for chromosome 11, with an average of 0.213 per locus. The genetic analysis of the 46 released Malaysian rice varieties using an unweighted pair group method with arithmetic mean (UPGMA) dendrogram revealed the presence of two major groups. The analysis was supported by the findings from the STRUCTURE analysis which indicated the ∆K value to be at the highest peak at K = 2, followed by K = 4. The pairwise genetic distance of the shared alleles showed that the value ranged from 0.000 (MR159 MR167) to 0.723 (MRIA Setanjung), which suggested that MR159 and MR167 were identical, and that the highest dissimilarity was detected between MRIA 1 and Setanjung. The results of the study will be very useful for the variety identification, the proper management and conservation of the genetic resources, and the exploitation and utilisation in future breeding programmes.","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":"56 1","pages":"62-70"},"PeriodicalIF":0.9,"publicationDate":"2020-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.17221/58/2019-cjgpb","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41578341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Ing. Pavel Bartoš, DrSc. − 90th birthday","authors":"A. Hanzalová, Hana Reimarová","doi":"10.17221/21/2020-cjgpb","DOIUrl":"https://doi.org/10.17221/21/2020-cjgpb","url":null,"abstract":"<jats:p />","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":"56 1","pages":"84-84"},"PeriodicalIF":0.9,"publicationDate":"2020-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.17221/21/2020-cjgpb","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47694645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shuiqin Li, H. Tang, Han Zhang, Y. Mu, X. Lan, Jian Ma
The 1BL/1RS wheat-rye translocation has been widely utilized in wheat genetic improvement and breeding programs. Our understanding on the effects of the 1BL/1RS translocation on wheat kernel size (e.g. length and width) is limited despite of numerous studies reporting about the effects on kernel weight. Here, we identified a wheat 1BL/1RS translocation line 88-1643 with higher kernel length (KL) using fluorescence in situ hybridization (FISH), genomic in situ hybridization (GISH) and molecular markers. To detect the possible role of the 1BL/1RS translocation in KL, kernel width (KW), and thousand-kernel weight (TKW), three recombinant inbred line (RIL) populations were constructed by crossing 88-1643 and three other wheat lines. As expected, the results showed that the values of KL in lines carrying 1RS were significantly higher than those carrying 1BS in three RIL populations at multiple environments, indicating that a major and stably expressed allele or gene responsible for increasing KL is most likely located on 1RS from 88-1643. Additionally, in one RIL population, the increased KL contributed significantly to the increase in TKW. Collectively, the 1BL/1RS translocation reported here is of interest to reveal molecular mechanism of the gene controlling KL and will be useful for improving wheat yield.
{"title":"A 1BL/1RS translocation contributing to kernel length increase in three wheat recombinant inbred line populations","authors":"Shuiqin Li, H. Tang, Han Zhang, Y. Mu, X. Lan, Jian Ma","doi":"10.17221/79/2019-cjgpb","DOIUrl":"https://doi.org/10.17221/79/2019-cjgpb","url":null,"abstract":"The 1BL/1RS wheat-rye translocation has been widely utilized in wheat genetic improvement and breeding programs. Our understanding on the effects of the 1BL/1RS translocation on wheat kernel size (e.g. length and width) is limited despite of numerous studies reporting about the effects on kernel weight. Here, we identified a wheat 1BL/1RS translocation line 88-1643 with higher kernel length (KL) using fluorescence in situ hybridization (FISH), genomic in situ hybridization (GISH) and molecular markers. To detect the possible role of the 1BL/1RS translocation in KL, kernel width (KW), and thousand-kernel weight (TKW), three recombinant inbred line (RIL) populations were constructed by crossing 88-1643 and three other wheat lines. As expected, the results showed that the values of KL in lines carrying 1RS were significantly higher than those carrying 1BS in three RIL populations at multiple environments, indicating that a major and stably expressed allele or gene responsible for increasing KL is most likely located on 1RS from 88-1643. Additionally, in one RIL population, the increased KL contributed significantly to the increase in TKW. Collectively, the 1BL/1RS translocation reported here is of interest to reveal molecular mechanism of the gene controlling KL and will be useful for improving wheat yield.","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2020-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.17221/79/2019-cjgpb","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46534818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The designation of traditional varieties of grapevine is usually based on verbal information or very dated records. Old rare cultivars found in the Czech Republic were identified by Simple Sequence Repeat (SSR) analysis, a generally accepted method for cultivar identification. These cultivars are primarily maintained in a national collection of genetic resources. Finally a total of 102 candidate genotypes was selected where 49 traditional varieties were identified on the base of nine SSR loci compared with the European Vitis Database. Thirty-six items were registered under the correct designation. The remaining genotypes included four clones, and two genotypes could be described as synonyms. Seven genotypes were found to be incorrectly marked. For three of them, the correct name was found in the database under their SSR profile and four items were considered to be unique as no identical profile was found.
{"title":"Identification of rare traditional grapevine cultivars using SSR markers and their geographical location within the Czech Republic","authors":"K. Baránková, R. Sotolář, M. Baránek","doi":"10.17221/61/2019-cjgpb","DOIUrl":"https://doi.org/10.17221/61/2019-cjgpb","url":null,"abstract":"The designation of traditional varieties of grapevine is usually based on verbal information or very dated records. Old rare cultivars found in the Czech Republic were identified by Simple Sequence Repeat (SSR) analysis, a generally accepted method for cultivar identification. These cultivars are primarily maintained in a national collection of genetic resources. Finally a total of 102 candidate genotypes was selected where 49 traditional varieties were identified on the base of nine SSR loci compared with the European Vitis Database. Thirty-six items were registered under the correct designation. The remaining genotypes included four clones, and two genotypes could be described as synonyms. Seven genotypes were found to be incorrectly marked. For three of them, the correct name was found in the database under their SSR profile and four items were considered to be unique as no identical profile was found.","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2020-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.17221/61/2019-cjgpb","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48566344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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