Pub Date : 2021-02-08DOI: 10.3727/036012921X16112663844842
Xiao-liang Wu, L. Ju, Ya-fang Song, Li-jun Bai, Menqian Yuan, Wanli Xu, Jing Li, Tian-Cheng Xu, Lixia Pei, Jian-hua Sun
The calcium-sensitive receptor (CaSR) plays a role in several biological processes. However, its role in intestinal motility remains unclear. In this study, we aimed to determine the effect of electro-acupuncture (EA) at Shangjuxu (ST37) on CaSR in colonic dysplasia mice, and to explore the possible mechanism of EA regulating colonic movement. The mice were injected with nicardipine or hexamethonium bromide to induce colonic dysplasia. Intestinal transit function was assessed by twelve hours fecal granules and fecal water content percentage, while colonic slow wave was assessed by multi-channel physiological signal acquisition system, immunofluorescence and laser confocal microscopy were used to examine CaSR expression in the gastrointestinal (GI) tract of the mice. We found that the number of fecal particles, the frequency and amplitude of colonic slow wave were disrupted after nicardipine or hexamethonium bromide injection. In addition, CaSR expression in control group was mainly distributed in intestinal epithelial cells, and the morphological structure of mucosal layer was regular. Compared with control group, the structure of mucosal layer in nicardipine group and hexamethonium bromide group were all disorderly, the expression and fluorescence intensity of CaSR in nicardipine group were visible, but in hexamethonium bromide group were weakened. After EA intervention, these disorders were ameliorated, which suggested that EA at ST37 could therefore regulate colonic motility disorders via the involvement of CaSR.
{"title":"Mechanism of Colonic Slow Wave Rhythm Regulated by Electro-acupuncture Determined using Calcium-Sensitive Receptor","authors":"Xiao-liang Wu, L. Ju, Ya-fang Song, Li-jun Bai, Menqian Yuan, Wanli Xu, Jing Li, Tian-Cheng Xu, Lixia Pei, Jian-hua Sun","doi":"10.3727/036012921X16112663844842","DOIUrl":"https://doi.org/10.3727/036012921X16112663844842","url":null,"abstract":"The calcium-sensitive receptor (CaSR) plays a role in several biological processes. However, its role in intestinal motility remains unclear. In this study, we aimed to determine the effect of electro-acupuncture (EA) at Shangjuxu (ST37) on CaSR in colonic dysplasia mice, and to explore\u0000 the possible mechanism of EA regulating colonic movement. The mice were injected with nicardipine or hexamethonium bromide to induce colonic dysplasia. Intestinal transit function was assessed by twelve hours fecal granules and fecal water content percentage, while colonic slow wave was assessed\u0000 by multi-channel physiological signal acquisition system, immunofluorescence and laser confocal microscopy were used to examine CaSR expression in the gastrointestinal (GI) tract of the mice. We found that the number of fecal particles, the frequency and amplitude of colonic slow wave were\u0000 disrupted after nicardipine or hexamethonium bromide injection. In addition, CaSR expression in control group was mainly distributed in intestinal epithelial cells, and the morphological structure of mucosal layer was regular. Compared with control group, the structure of mucosal layer in\u0000 nicardipine group and hexamethonium bromide group were all disorderly, the expression and fluorescence intensity of CaSR in nicardipine group were visible, but in hexamethonium bromide group were weakened. After EA intervention, these disorders were ameliorated, which suggested that EA at\u0000 ST37 could therefore regulate colonic motility disorders via the involvement of CaSR.","PeriodicalId":50881,"journal":{"name":"Acupuncture & Electro-Therapeutics Research","volume":"45 1","pages":"47-58"},"PeriodicalIF":0.3,"publicationDate":"2021-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43024914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-02-08DOI: 10.3727/036012921X16112663844923
Zahra Tavakol, A. Shariat, N. N. Ansari, Shima Ghannadi, Roshanak Honarpishe, J. Dommerholt, P. Noormohammadpour, L. Ingle
Spasticity is one of the main complications of a stroke. This double-blind, randomized controlled trial aimed to compare the result of three sessions of dry needling (DN) versus sham DN on the affected upper limbs in post-stroke survivors. We recruited 24 patients (age 57.0 ± 9.6 years; male 71%). Patients were randomly allocated to two groups: a DN group and a sham DN group. The primary outcome measures were the Modified Modified Ashworth Scale (MMAS) and the Box and Block Test (BBT). Secondary outcome measures included active and passive wrist range of motion (AROM and PROM). All assessments were measured at baseline, immediately after the last session of the intervention, and one month later. Patients in the DN group had improved upper limb spasticity and passive wrist range of motion compared to control group (P < 0.05). There were no between-group differences in other outcome measures (P > 0.05). Dry needling is a useful method for improving muscle spasticity in the upper limbs of patients with stroke.
痉挛是中风的主要并发症之一。这项双盲、随机对照试验旨在比较中风后幸存者上肢受影响的三个疗程的干针(DN)和假DN的结果。我们招募了24例患者(年龄57.0±9.6岁;男性的71%)。患者被随机分为两组:DN组和假性DN组。主要评价指标为改良改良Ashworth量表(MMAS)和Box and Block Test (BBT)。次要结果测量包括主动和被动手腕活动范围(AROM和PROM)。所有的评估都是在基线、最后一次干预后立即和一个月后进行的。与对照组相比，DN组患者上肢痉挛和被动腕关节活动度改善(P < 0.05)。其他结局指标组间差异无统计学意义(P < 0.05)。干针是改善中风患者上肢肌肉痉挛的一种有效方法。
{"title":"A Double-blind Randomized Controlled Trial for the Effects of Dry Needling on Upper Limb Dysfunction in Patients with Stroke","authors":"Zahra Tavakol, A. Shariat, N. N. Ansari, Shima Ghannadi, Roshanak Honarpishe, J. Dommerholt, P. Noormohammadpour, L. Ingle","doi":"10.3727/036012921X16112663844923","DOIUrl":"https://doi.org/10.3727/036012921X16112663844923","url":null,"abstract":"Spasticity is one of the main complications of a stroke. This double-blind, randomized controlled trial aimed to compare the result of three sessions of dry needling (DN) versus sham DN on the affected upper limbs in post-stroke survivors. We recruited 24 patients (age 57.0 ±\u0000 9.6 years; male 71%). Patients were randomly allocated to two groups: a DN group and a sham DN group. The primary outcome measures were the Modified Modified Ashworth Scale (MMAS) and the Box and Block Test (BBT). Secondary outcome measures included active and passive wrist range of motion\u0000 (AROM and PROM). All assessments were measured at baseline, immediately after the last session of the intervention, and one month later. Patients in the DN group had improved upper limb spasticity and passive wrist range of motion compared to control group (P < 0.05). There were\u0000 no between-group differences in other outcome measures (P > 0.05). Dry needling is a useful method for improving muscle spasticity in the upper limbs of patients with stroke.","PeriodicalId":50881,"journal":{"name":"Acupuncture & Electro-Therapeutics Research","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2021-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47113475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-02-08DOI: 10.3727/036012921X16112663844932
Wang Fan, Fan Shendong, Cui Guangwei, C. Huaijin, Shi She, Wang Qiangli, W. Lina, Shen Xueyong
Objectives: To study the mechanism of acupuncture manipulation (AM) based on the secretion function and morphological variation of fibroblasts in acupoint region. Methods: 40 rabbits were randomly divided to normal group (N), model group (M), needle retaining group (A), and acupuncture manipulation group (AM), each group consist of 10 rabbits. The animal model of Rabbits with Bacterium Endotoxin Induced Fever (BEIF) was established by intravenous injection of bacterial endotoxin. Groups A and AM were treated with 'Qu Chi'(LI11) acupuncture after modeling, and acupuncture manipulations were conducted in group AM. The serum heat factors IL-1β, IL-6, and TNF-α content were assayed conducted in group AM. The serum heat factors IL-1β, IL-6, and TNF-α content were assayed by Elisa after acupuncturet; the morphological changes of fibroblasts in acupoint area were observed by Vimentin staining; the fibroblasts of subcutaneous connective tissue in acupoint area were isolated and cultured in vitro, and the contents of PEG2, NO and ATP in supernatant were assayed. Results: There was no significant difference in fibroblasts cytomorphology among groups M, A, and N. Fibroblasts in group AM were stretched and aligned in almost one direction. Comparing with group N, the content of serum IL-1β, IL-6, and TNF-α was significantly higher in group M, along with higher NO and ATP content in the cell culture supernant; Comparing with group M, content of IL-6 and TNF-α was lower in group A, content of serum IL-1β, IL-6 and TNF-α was higher in group AM, along with higher PEG2, NO and ATP content in both groups, while group AM demonstrated more significant changes in the above indicators than group A. Conclusion: Acupuncture had therapeutic effects on BEIF rabbits, the application of acupuncture manipulation can further improve its therapeutic effects. The mechanism may be related to the influence on fibroblasts cytomorphology in acupoint region and facilitation of fibroblasts-derived PEG2, NO, and ATP, which together promote the acupoint vitality.
{"title":"Skin fibroblasts' alteration after Acupuncture on LI11 in Rabbits with bacteria endotoxin induced fever","authors":"Wang Fan, Fan Shendong, Cui Guangwei, C. Huaijin, Shi She, Wang Qiangli, W. Lina, Shen Xueyong","doi":"10.3727/036012921X16112663844932","DOIUrl":"https://doi.org/10.3727/036012921X16112663844932","url":null,"abstract":"Objectives: To study the mechanism of acupuncture manipulation (AM) based on the secretion function and morphological variation of fibroblasts in acupoint region. Methods: 40 rabbits were randomly divided to normal group (N), model group (M), needle retaining\u0000 group (A), and acupuncture manipulation group (AM), each group consist of 10 rabbits. The animal model of Rabbits with Bacterium Endotoxin Induced Fever (BEIF) was established by intravenous injection of bacterial endotoxin. Groups A and AM were treated with 'Qu Chi'(LI11) acupuncture after\u0000 modeling, and acupuncture manipulations were conducted in group AM. The serum heat factors IL-1β, IL-6, and TNF-α content were assayed conducted in group AM. The serum heat factors IL-1β, IL-6, and TNF-α content were assayed by Elisa after acupuncturet; the morphological\u0000 changes of fibroblasts in acupoint area were observed by Vimentin staining; the fibroblasts of subcutaneous connective tissue in acupoint area were isolated and cultured in vitro, and the contents of PEG2, NO and ATP in supernatant were assayed. Results: There was no significant\u0000 difference in fibroblasts cytomorphology among groups M, A, and N. Fibroblasts in group AM were stretched and aligned in almost one direction. Comparing with group N, the content of serum IL-1β, IL-6, and TNF-α was significantly higher in group M, along with higher NO and ATP content\u0000 in the cell culture supernant; Comparing with group M, content of IL-6 and TNF-α was lower in group A, content of serum IL-1β, IL-6 and TNF-α was higher in group AM, along with higher PEG2, NO and ATP content in both groups, while group AM demonstrated more significant changes\u0000 in the above indicators than group A. Conclusion: Acupuncture had therapeutic effects on BEIF rabbits, the application of acupuncture manipulation can further improve its therapeutic effects. The mechanism may be related to the influence on fibroblasts cytomorphology in\u0000 acupoint region and facilitation of fibroblasts-derived PEG2, NO, and ATP, which together promote the acupoint vitality.","PeriodicalId":50881,"journal":{"name":"Acupuncture & Electro-Therapeutics Research","volume":"45 1","pages":"125-136"},"PeriodicalIF":0.3,"publicationDate":"2021-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45976973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-02-08DOI: 10.3727/036012921X16112663844950
Chen Liu
Circulating tumor cells (CTC) enumeration has been clinically adopted as important prognostic factor in different cancers, but many of CTCs isolation or identification apporches did not have clinical data. In this study, we developed a novel indirect immunofluorescent staining method targeting EpCAM or cytokeratin for CTC identification. Peripheral blood samples from twenty healthy donors and thirty-six cancer patients were used for cutoff establishment and sensitivity test. An immunofluorescent intensity threshold value of 5080 for the EpCAM or cytokeratin staining led to highly accurate cell classification (95% sensitivity and >99% specificity), and with the cut-off point of 2 CTCs, the sensitivity of CTC detection was 54.2% (19/35) in the diagnosis of patients with different cancer. Besides, the presence of CTCs defined as having >2 CTCs/7.5 mL might be associated with cancer staging and occurred three weeks earlier than cancer relapse in a breast cancer patient, and thus was consider a clinically useful tool to monitor tumor recurrence during chemotherapy.
{"title":"Sensitivity of a novel immunofluorescent staining method for Circulating tumor cells identification and its clinical application in predicting cancer recurrence in breast cancer","authors":"Chen Liu","doi":"10.3727/036012921X16112663844950","DOIUrl":"https://doi.org/10.3727/036012921X16112663844950","url":null,"abstract":"Circulating tumor cells (CTC) enumeration has been clinically adopted as important prognostic factor in different cancers, but many of CTCs isolation or identification apporches did not have clinical data. In this study, we developed a novel indirect immunofluorescent staining method\u0000 targeting EpCAM or cytokeratin for CTC identification. Peripheral blood samples from twenty healthy donors and thirty-six cancer patients were used for cutoff establishment and sensitivity test. An immunofluorescent intensity threshold value of 5080 for the EpCAM or cytokeratin staining led\u0000 to highly accurate cell classification (95% sensitivity and >99% specificity), and with the cut-off point of 2 CTCs, the sensitivity of CTC detection was 54.2% (19/35) in the diagnosis of patients with different cancer. Besides, the presence of CTCs defined as having >2 CTCs/7.5 mL might\u0000 be associated with cancer staging and occurred three weeks earlier than cancer relapse in a breast cancer patient, and thus was consider a clinically useful tool to monitor tumor recurrence during chemotherapy.","PeriodicalId":50881,"journal":{"name":"Acupuncture & Electro-Therapeutics Research","volume":"45 1","pages":"137-146"},"PeriodicalIF":0.3,"publicationDate":"2021-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43195946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-02-08DOI: 10.3727/036012921X16112663844879
W. Jian, Z. Can, Yang Jun, Xin Liwei, Zhang Kun, Tai Xiantao
Objectives To explore the effects of electroacupuncture on nerve function in rats with ischemic stroke and its mechanism of anti-apoptosis. Methods A total of 80 SPF male SD rats were randomly divided into sham operation group, model group, sham electroacupuncture group, acupuncture group, electroacupuncture group and 16 rats in each group. The rat model of left ischemic stroke was prepared by suture embolization. In the sham group, the left common carotid artery was isolated only and no other treatment was given. In the electroacupuncture group, "Baihui" and "Mingmen" were selected for acupuncture, followed by dilatation wave, frequency 2Hz/100Hz, intensity 1mA, and electroacupuncture for 30min. The sham electroacupuncture group was the same as the electroacupuncture group in acupoint electroacupuncture group was the same as the electroacupuncture group in acupoint selection. The electroacupuncture group was only inserted subcutaneously and then connected with the electroacupuncture group without power supply, and fixed for 30 minutes. The electroacupuncture group and the acupuncture group received electroacupuncture treatment once, for a total of 14 days, 1d after modeling. The Improved Neurological Impairment scale (mNSS) was used to evaluate the degree of neurological impairment in each group after anesthesia and wakefulness. The percentage of cerebral infarction area was determined by TTC staining. HE staining and Nissl staining were used to observe the pathological changes of ischemic brain tissue. The level of apoptosis in ischemic brain tissue was detected by TUNEL assay. Western blot was used to detect protein expression of Bax, Bcl-2 and Cleaved caspase-3 in ischemic brain tissue. Results Compared with the sham operation group, neurological function score, percentage of cerebral infarction area and apoptosis level in the model group were significantly increased (all P < 0.01). Compared with the model group, neurological function score, percentage of cerebral infarction area and apoptosis index of acupuncture group and electroacupuncture group were decreased (all P <0.05). Compared with the model group, the expression levels of Bcl-2 protein in ischemic brain tissue of rats with ischemic stroke were up-regulated in the acupuncture group and electroacupuncture group to different degrees, while the expression levels of Bax and Cleaved caspase-3 protein were down-regulated in the electroacupuncture group. Conclusion Electroacupuncture may inhibit Bax, Cleaved caspase-3 and up-regulate the expression of Bcl-2 against neuronal apoptosis, thereby improving the neurological function injury of ischemic stroke rats.
{"title":"Effects of near-far acupuncture on neuronal function and expression of apoptosis-related protein Bax/Bcl-2/Cleaved caspase-3 in rats with ischemic stroke","authors":"W. Jian, Z. Can, Yang Jun, Xin Liwei, Zhang Kun, Tai Xiantao","doi":"10.3727/036012921X16112663844879","DOIUrl":"https://doi.org/10.3727/036012921X16112663844879","url":null,"abstract":"Objectives To explore the effects of electroacupuncture on nerve function in rats with ischemic stroke and its mechanism of anti-apoptosis. Methods A total of 80 SPF male SD rats were randomly divided into sham operation group, model group, sham electroacupuncture group,\u0000 acupuncture group, electroacupuncture group and 16 rats in each group. The rat model of left ischemic stroke was prepared by suture embolization. In the sham group, the left common carotid artery was isolated only and no other treatment was given. In the electroacupuncture group, \"Baihui\"\u0000 and \"Mingmen\" were selected for acupuncture, followed by dilatation wave, frequency 2Hz/100Hz, intensity 1mA, and electroacupuncture for 30min. The sham electroacupuncture group was the same as the electroacupuncture group in acupoint electroacupuncture group was the same as the\u0000 electroacupuncture group in acupoint selection. The electroacupuncture group was only inserted subcutaneously and then connected with the electroacupuncture group without power supply, and fixed for 30 minutes. The electroacupuncture group and the acupuncture group received electroacupuncture\u0000 treatment once, for a total of 14 days, 1d after modeling. The Improved Neurological Impairment scale (mNSS) was used to evaluate the degree of neurological impairment in each group after anesthesia and wakefulness. The percentage of cerebral infarction area was determined by TTC staining.\u0000 HE staining and Nissl staining were used to observe the pathological changes of ischemic brain tissue. The level of apoptosis in ischemic brain tissue was detected by TUNEL assay. Western blot was used to detect protein expression of Bax, Bcl-2 and Cleaved caspase-3 in ischemic brain tissue.\u0000 Results Compared with the sham operation group, neurological function score, percentage of cerebral infarction area and apoptosis level in the model group were significantly increased (all P < 0.01). Compared with the model group, neurological function score, percentage of\u0000 cerebral infarction area and apoptosis index of acupuncture group and electroacupuncture group were decreased (all P <0.05). Compared with the model group, the expression levels of Bcl-2 protein in ischemic brain tissue of rats with ischemic stroke were up-regulated in the acupuncture\u0000 group and electroacupuncture group to different degrees, while the expression levels of Bax and Cleaved caspase-3 protein were down-regulated in the electroacupuncture group. Conclusion Electroacupuncture may inhibit Bax, Cleaved caspase-3 and up-regulate the expression of Bcl-2 against\u0000 neuronal apoptosis, thereby improving the neurological function injury of ischemic stroke rats.","PeriodicalId":50881,"journal":{"name":"Acupuncture & Electro-Therapeutics Research","volume":"45 1","pages":"73-86"},"PeriodicalIF":0.3,"publicationDate":"2021-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47290355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-02-08DOI: 10.3727/036012921X16112663844897
Linjun Li, Mengjie Zeng, Zhi-jia Wu
To observe the difference in clinical effect of Scalp cluster acupuncture on cervical spondylosis of vertebral artery type. Using a randomized control design, 96 patients with vertebral artery cervical spondylopathy were randomly divided into two groups: conventional acupuncture point combined with Scalp cluster acupuncture group (treatment group) 48 cases, conventional acupoint group (control group) 48 cases. After 2 courses of treatment, it was judged by observing clinical efficacy evaluation, DARS evaluation, and VAS pain scale. The total effective rate was 93.8% in the treatment group and 79.2% in the control group; the difference between the two groups was statistically significant (P < 0.05). Before treatment, the difference in DARS between the two groups was not statistically significant (P> 0.05); after treatment, the difference between the two groups was statistically significant (P < 0.01) ; After treatment, the difference between the two groups was statistically significant (P < 0.05). The VAS scores of the two groups of patients before treatment were comparable (P> 0.05), and there were significant differences between the two groups after treatment (P <0.05);The difference of VAS score between the two groups was statistically significant (P < 0.01). Scalp cluster acupuncture therapy is more effective than traditional acupuncture therapy alone in treating vertebral artery type cervical spondylosis.
{"title":"Observation on clinical effect of Scalp cluster acupuncture treatment of cervical spondylosis of vertebral artery type","authors":"Linjun Li, Mengjie Zeng, Zhi-jia Wu","doi":"10.3727/036012921X16112663844897","DOIUrl":"https://doi.org/10.3727/036012921X16112663844897","url":null,"abstract":"To observe the difference in clinical effect of Scalp cluster acupuncture on cervical spondylosis of vertebral artery type. Using a randomized control design, 96 patients with vertebral artery cervical spondylopathy were randomly divided into two groups: conventional acupuncture point\u0000 combined with Scalp cluster acupuncture group (treatment group) 48 cases, conventional acupoint group (control group) 48 cases. After 2 courses of treatment, it was judged by observing clinical efficacy evaluation, DARS evaluation, and VAS pain scale. The total effective rate was 93.8% in\u0000 the treatment group and 79.2% in the control group; the difference between the two groups was statistically significant (P < 0.05). Before treatment, the difference in DARS between the two groups was not statistically significant (P> 0.05); after treatment, the difference between the\u0000 two groups was statistically significant (P < 0.01) ; After treatment, the difference between the two groups was statistically significant (P < 0.05). The VAS scores of the two groups of patients before treatment were comparable (P> 0.05), and there were significant differences between\u0000 the two groups after treatment (P <0.05);The difference of VAS score between the two groups was statistically significant (P < 0.01). Scalp cluster acupuncture therapy is more effective than traditional acupuncture therapy alone in treating vertebral artery type cervical spondylosis.","PeriodicalId":50881,"journal":{"name":"Acupuncture & Electro-Therapeutics Research","volume":"45 1","pages":"87-96"},"PeriodicalIF":0.3,"publicationDate":"2021-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44364952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-02-08DOI: 10.3727/036012921X16112663844851
Hidero Takahashi, T. Nabeta, H. Waki, K. Takeoka, Hisahiro Kawahata, Norio Ideguchi, Motokuni Aoki, T. Ogihara
Acupuncture, which is widely used as a complementary and alternative medicine, has been expected to be beneficial for the treatment of hypertension. However, the antihypertensive effect of acupuncture is still under debate. In the present study, the blood pressure induced by handgrip stress were evaluated using power spectral frequency analysis and hormone examination. This study, which recruited 6 normotensive participants, was designed as a randomized crossover trial of two interventions; electroacupuncture and sham-electroacupuncture. Intervention was delivered as 5 sessions over 2 consecutive weeks. The first electroacupuncture immediately before handgrip exercise did not affect the rates of increase in blood pressure and heart rate. Also, neither increase in LF/HF ratio, as sympathetic nerve activity, nor decrease in HF value, as parasympathetic nerve activity, by handgrip stress was significantly inhibited by a single intervention of electroacupuncture. In addition, 5 repeated electroacupuncture sessions did not attenuate the elevation of blood pressure after handgrip stress, and did not suppress the increase in LF/HF ratio and decrease in HF value. Also, electroacupuncture did not influence the changes in plasma concentrations of renin, aldosterone, norepinephrine, and epinephrine after handgrip stress. No difference was observed in the rate of change in each parameter after handgrip stress between the electroacupuncture treatment group and the sham-electroacupuncture group. The present study did not show any suppressive effect on stress-induced response of blood pressure as well as sympathetic nerve activity. The obtained findings showed no beneficial effects of acupuncture on hypertension as a standalone therapy, consistent with suggestions by previous systematic reviews and meta-analyses. Research with larger sample sizes or higher-quality randomized controlled trials are expected, and a conclusion on the utility of acupuncture treatment for hypertension should be carefully made.
{"title":"Effect of Electroacupuncture on Stress-induced High Blood Pressure via Autonomic Nervous System in Normotensive Young Adults","authors":"Hidero Takahashi, T. Nabeta, H. Waki, K. Takeoka, Hisahiro Kawahata, Norio Ideguchi, Motokuni Aoki, T. Ogihara","doi":"10.3727/036012921X16112663844851","DOIUrl":"https://doi.org/10.3727/036012921X16112663844851","url":null,"abstract":"Acupuncture, which is widely used as a complementary and alternative medicine, has been expected to be beneficial for the treatment of hypertension. However, the antihypertensive effect of acupuncture is still under debate. In the present study, the blood pressure induced by handgrip\u0000 stress were evaluated using power spectral frequency analysis and hormone examination. This study, which recruited 6 normotensive participants, was designed as a randomized crossover trial of two interventions; electroacupuncture and sham-electroacupuncture. Intervention was delivered as 5\u0000 sessions over 2 consecutive weeks. The first electroacupuncture immediately before handgrip exercise did not affect the rates of increase in blood pressure and heart rate. Also, neither increase in LF/HF ratio, as sympathetic nerve activity, nor decrease in HF value, as parasympathetic nerve\u0000 activity, by handgrip stress was significantly inhibited by a single intervention of electroacupuncture. In addition, 5 repeated electroacupuncture sessions did not attenuate the elevation of blood pressure after handgrip stress, and did not suppress the increase in LF/HF ratio and decrease\u0000 in HF value. Also, electroacupuncture did not influence the changes in plasma concentrations of renin, aldosterone, norepinephrine, and epinephrine after handgrip stress. No difference was observed in the rate of change in each parameter after handgrip stress between the electroacupuncture\u0000 treatment group and the sham-electroacupuncture group. The present study did not show any suppressive effect on stress-induced response of blood pressure as well as sympathetic nerve activity. The obtained findings showed no beneficial effects of acupuncture on hypertension as a standalone\u0000 therapy, consistent with suggestions by previous systematic reviews and meta-analyses. Research with larger sample sizes or higher-quality randomized controlled trials are expected, and a conclusion on the utility of acupuncture treatment for hypertension should be carefully made.","PeriodicalId":50881,"journal":{"name":"Acupuncture & Electro-Therapeutics Research","volume":"45 1","pages":"59-72"},"PeriodicalIF":0.3,"publicationDate":"2021-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48185238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-02-08DOI: 10.3727/036012921X16112663844905
H. Alp
Obesity is a disease defined by excessive fat storage in the body. It is an energy balance problem; the increase in body fat is caused by an imbalance between energy intake and energy expenditure. This study aimed to determinate the changes in the anthropometric measurements of patients after acupuncture treatment used in obesity treatment. The present study was a cross-sectional study conducted in 2019 in xxx University's GETAT Center). Patients aged between 18–65 years with a BMI > 25 were included in the study. The participants were divided into two groups: Group 1 (acupuncture and diet) and Group 2 (diet only). Exclusion criteria: Pregnant women, breastfeeding patients , susceptible demographics (acute coronary insufficiency, immunodeficiency, severe psychotic disorder, liver and kidney failure), and those who could not give consent were not included in the study. Weight, BMI, and metabolic age values were measured with a Tanita device; the same diagnostic device was used for all patients. Both body and ear acupuncture were performed. In patients with all acupuncture, the yuan points of abnormal meridians were used for pulse diagnosis. ST 24, 25, and 36 (ZuSanLi); GV 20, 21, 5, 5, and LR 3 (Taichong); SP 6 and 9, GB 34, UB 62, HT 7, LU 9, and PC 6 (Nei Guan); and SI 3 and EXT-HN 3 (Yin-Tang) points were dewed. For ear acupuncture, hunger, kidney, larynx, stomach, jerome, and shen-men points were taken. A t-test was applied to pre- and post-acupuncture measurement values. After acupuncture, the waist circumference of the participants decreased significantly (p < 0.005). The BMI of the participants also decreased significantly after acupuncture (p < 0.005). In these studies, LU 6, ST 40, ST 21, K 4, ST 36 and 25, and LI 11 body points were used, while hunger, stomach, and shen-men points were commonly used as ear points.
肥胖是一种由体内脂肪储存过多所定义的疾病。这是一个能量平衡问题;身体脂肪的增加是由能量摄入和能量消耗之间的不平衡引起的。本研究旨在确定针灸治疗肥胖后患者人体测量值的变化。本研究是2019年在xxx大学GETAT中心进行的一项横断面研究。年龄在18-65岁之间，BMI为bb25的患者被纳入研究。将受试者分为两组:第一组(针灸+饮食)和第二组(仅饮食)。排除标准:孕妇、哺乳期患者、易感人群(急性冠状动脉功能不全、免疫缺陷、严重精神障碍、肝肾功能衰竭)和不能给予同意者不在研究范围内。用Tanita装置测量体重、BMI和代谢年龄值;所有患者使用相同的诊断设备。采用体针和耳针。所有针刺患者均采用异常经络元穴进行脉象诊断。st24,25和36(祖三李);GV 20、21、5、5和lr3(台中);sp6和9、gb34、ub62、ht7、lu9、pc6(内关);SI - 3和ext - hn3(阴堂)点露珠。耳穴取饥穴、肾穴、喉穴、胃穴、肾穴、肾门穴。针刺前后测量值采用t检验。针刺后，受试者腰围明显减小(p < 0.005)。针刺后受试者的BMI也显著降低(p < 0.005)。在这些研究中，使用了LU 6, ST 40, ST 21, k4, ST 36和25，以及LI 11体穴，而饥饿，胃和深门穴常用作为耳穴。
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Pub Date : 2021-02-01DOI: 10.3727/036012921x16128784949502
Anonymous
The proceedings contain 56 papers. The topics discussed include: BDORT evaluation in COVID-19 cases: researching severity markers and predictive indexes;evaluation using BDORT resonance phenomenon between two identical substances of H5N1, H1N1 and SARSCOV2 viruses;resonance for COVID-19 evaluated by BDORT in obstetric ultrasound examinations;neuropathy of the optical nerve, and the BDORT. Case report;imaging patterns at COVID-19: drawing a new pathology by BDORT technique;COVID-19 encephalopathy: challenges for post-pandemic times;thymus dysfunction in individuals with resonance for the new coronavirus;acupuncture for acute non-specific low back pain: a randomized, controlled, double-blind, placebo trial;and auricular chromotherapy in the treatment of psychological trauma, phobias and panic disorder.
{"title":"Abstracts of The 36th Annual International Symposium on Acupuncture, Electrotherapeutics, & Latest Advancements","authors":"Anonymous","doi":"10.3727/036012921x16128784949502","DOIUrl":"https://doi.org/10.3727/036012921x16128784949502","url":null,"abstract":"The proceedings contain 56 papers. The topics discussed include: BDORT evaluation in COVID-19 cases: researching severity markers and predictive indexes;evaluation using BDORT resonance phenomenon between two identical substances of H5N1, H1N1 and SARSCOV2 viruses;resonance for COVID-19 evaluated by BDORT in obstetric ultrasound examinations;neuropathy of the optical nerve, and the BDORT. Case report;imaging patterns at COVID-19: drawing a new pathology by BDORT technique;COVID-19 encephalopathy: challenges for post-pandemic times;thymus dysfunction in individuals with resonance for the new coronavirus;acupuncture for acute non-specific low back pain: a randomized, controlled, double-blind, placebo trial;and auricular chromotherapy in the treatment of psychological trauma, phobias and panic disorder.","PeriodicalId":50881,"journal":{"name":"Acupuncture & Electro-Therapeutics Research","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2021-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48258689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Catgut implantation at acupoints (CIA) has a long history as a medical treatment for a wide variety of diseases, including autoimmune diseases. However, the effect and mechanism of this therapy in autoimmune uveitis is still largely unknown. The aim of this study was to explore the immunity-inhibitory effect of CIA in an experimental autoimmune uveitis (EAU) rat model. EAU was established in Lewis rats by the injection of IRBP1177–1191 peptide. The rats were randomly divided into control and CIA groups. Phenotypic and histological assessments were performed days 9, 13, 18, 23 post-immunization. The percentage of Th1 and Th17 lymphocytes isolated from lymph nodes were determined by flow cytometry. The expression of IL-17 and IFN-γ was detected by real-time quantitative PCR and enzyme-linked immunosorbent assay (ELISA). In the CIA group, delayed mild inflammation was observed. Pathological investigation found alleviated infiltration of lymphocytes and ocular damage. Flow cytometry showed significantly decreased Th17 lymphocytes at day 9, 13, and 18 post-immunization (P<0.05) and no significant changes at day 23 post-immunization (P=0.868) after CIA. The Th1 lymphocytes were significantly decreased at day 13 and 18 post-immunization (P<0.05) and comparable at day 9 (P=0.111) and 23 (P=0.551) post-immunization in the CIA group. IL-17 and IFN-γ mRNA levels were notably decreased at day 9, 13 and 18 post-immunization (P<0.05) and showed a downward trend at day 23 post-immunization, although with no significance (P=0.080 and P=0.137, respectively) after CIA. Serum IL-17 and IFN-γ levels in the CIA group were significantly decreased at day 9, 13 and 18 post-immunization (P<0.05) and were comparable at day 23 post-immunization (P=0.078 and P=0.979, respectively). Ocular inflammation was markedly inhibited after catgut implantation at Pishu (BL20) and Shenshu (BL23) acupoints in an EAU rat model. Moreover, CIA reduced Th1 and Th17 lymphocytes and the expression IFN-γ and IL-17.
{"title":"Catgut Implantation at Acupoints has an Immunosuppressive Effect on Autoimmune Uveitis by Regulating Th1/Th17 Lymphocytes","authors":"Feifei Chen, Jianying Zhao, Shuyang Zhong, Fengming Zheng, Xiaobo Hao","doi":"10.3727/036012921x16237619666012","DOIUrl":"https://doi.org/10.3727/036012921x16237619666012","url":null,"abstract":"Catgut implantation at acupoints (CIA) has a long history as a medical treatment for a wide variety of diseases, including autoimmune diseases. However, the effect and mechanism of this therapy in autoimmune uveitis is still largely unknown. The aim of this study was to explore the immunity-inhibitory effect of CIA in an experimental autoimmune uveitis (EAU) rat model. EAU was established in Lewis rats by the injection of IRBP1177–1191 peptide. The rats were randomly divided into control and CIA groups. Phenotypic and histological assessments were performed days 9, 13, 18, 23 post-immunization. The percentage of Th1 and Th17 lymphocytes isolated from lymph nodes were determined by flow cytometry. The expression of IL-17 and IFN-γ was detected by real-time quantitative PCR and enzyme-linked immunosorbent assay (ELISA). In the CIA group, delayed mild inflammation was observed. Pathological investigation found alleviated infiltration of lymphocytes and ocular damage. Flow cytometry showed significantly decreased Th17 lymphocytes at day 9, 13, and 18 post-immunization (P<0.05) and no significant changes at day 23 post-immunization (P=0.868) after CIA. The Th1 lymphocytes were significantly decreased at day 13 and 18 post-immunization (P<0.05) and comparable at day 9 (P=0.111) and 23 (P=0.551) post-immunization in the CIA group. IL-17 and IFN-γ mRNA levels were notably decreased at day 9, 13 and 18 post-immunization (P<0.05) and showed a downward trend at day 23 post-immunization, although with no significance (P=0.080 and P=0.137, respectively) after CIA. Serum IL-17 and IFN-γ levels in the CIA group were significantly decreased at day 9, 13 and 18 post-immunization (P<0.05) and were comparable at day 23 post-immunization (P=0.078 and P=0.979, respectively). Ocular inflammation was markedly inhibited after catgut implantation at Pishu (BL20) and Shenshu (BL23) acupoints in an EAU rat model. Moreover, CIA reduced Th1 and Th17 lymphocytes and the expression IFN-γ and IL-17.","PeriodicalId":50881,"journal":{"name":"Acupuncture & Electro-Therapeutics Research","volume":"1 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69698438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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