Pub Date : 2025-01-03DOI: 10.1016/j.bioadv.2025.214174
Yan Gan , Haotian Han , Ying Zhang , Ziwei Zhou , Xiang Shen , Jianjun Fang , Lei Cui , Zhihua Zhou
Chitosan is a promising biomaterial for tissue engineering, but its functionality is limited by a lack of bioactive sites. This study develops chitosan/amniotic membrane microcarriers to enhance vascularization and tissue regeneration for subcutaneous adipose tissue. The incorporation of decellularized amniotic membrane enhances the bioactivities of chitosan in promoting cell differentiation and angiogenesis. Optimized preparation yielded porous microcarriers with a particle size of 261.2 ± 28 μm and an average pore size of 19.0 ± 4 μm. In vitro degradation analysis showed accelerated degradation with higher amniotic membrane content. Cytocompatibility and adipogenic capacity assessments indicated that the microcarriers supported cell adhesion and proliferation over 7 days, with amniotic membrane facilitating adipogenic differentiation of adipose-derived stem cells. When injected subcutaneously into nude mice, these microcarriers formed neoplastic adipose tissues, which were harvested 8 weeks later. Fluorescence staining, oil-red O staining and CD31 labeling demonstrated that amniotic membrane incorporation significantly enhanced in vivo adipose tissue formation and angiogenesis.
{"title":"Chitosan-based injectable porous microcarriers with enhanced adipogenic differentiation and angiogenesis for subcutaneous adipose tissue regeneration","authors":"Yan Gan , Haotian Han , Ying Zhang , Ziwei Zhou , Xiang Shen , Jianjun Fang , Lei Cui , Zhihua Zhou","doi":"10.1016/j.bioadv.2025.214174","DOIUrl":"10.1016/j.bioadv.2025.214174","url":null,"abstract":"<div><div>Chitosan is a promising biomaterial for tissue engineering, but its functionality is limited by a lack of bioactive sites. This study develops chitosan/amniotic membrane microcarriers to enhance vascularization and tissue regeneration for subcutaneous adipose tissue. The incorporation of decellularized amniotic membrane enhances the bioactivities of chitosan in promoting cell differentiation and angiogenesis. Optimized preparation yielded porous microcarriers with a particle size of 261.2 ± 28 μm and an average pore size of 19.0 ± 4 μm. In vitro degradation analysis showed accelerated degradation with higher amniotic membrane content. Cytocompatibility and adipogenic capacity assessments indicated that the microcarriers supported cell adhesion and proliferation over 7 days, with amniotic membrane facilitating adipogenic differentiation of adipose-derived stem cells. When injected subcutaneously into nude mice, these microcarriers formed neoplastic adipose tissues, which were harvested 8 weeks later. Fluorescence staining, oil-red O staining and CD31 labeling demonstrated that amniotic membrane incorporation significantly enhanced in vivo adipose tissue formation and angiogenesis.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"169 ","pages":"Article 214174"},"PeriodicalIF":5.5,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142933495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abdominal hernia repair is a common surgical procedure, involving in most cases the use of textile meshes providing a mechanical barrier to consolidate the damaged surrounding tissues and prevent the resurgence of the hernia. However, in more than half cases postoperative complications such as adhesions and infections occur at the surface of the mesh, leading to chronic pain for the patient and requiring the removal of the implant. One of the most promising strategies to reduce the risk of postoperative adhesions and infections is to add a physical barrier between the mesh and the abdominal walls. In this study, we propose a strategy to develop functional hernia meshes possessing anticoagulant and antibacterial activities depending on the side of the implant. Two bioactive polymers were synthetized: a polysulfonate (poly(2-acrylamido-2-methylpropane sulfonic acid), PAMPS) one for anticoagulant activity and a polymer bearing ternary amines (poly((2-tert-butylamino) ethyl methacrylate), PTBAEMA) for antibacterial activity. These polymers were used to produce core-sheath nanofibers thanks to coaxial electrospinning with poly(ɛ-caprolactone) (PCL) as core and the bioactive polymer as sheath. The electrospinning parameters were optimized in order to obtain defect-free nanofibrous coatings onto the mesh with improved stability in water. The core-sheath structure was investigated as well as the presence of the functional groups at the surface. The in vitro cytocompatibility, anticoagulant activity and antibacterial activity were evaluated and highlighted the high potential of these coatings for the simultaneous prevention of postoperative adhesions and infections.
{"title":"Abdominal PP meshes coated with functional core-sheath biodegradable nanofibers with anticoagulant and antibacterial properties","authors":"Malo Dufay , Maude Jimenez , Mathilde Casetta , Feng Chai , Nicolas Blanchemain , Mickael Maton , Frédéric Cazaux , Séverine Bellayer , Stéphanie Degoutin","doi":"10.1016/j.bioadv.2024.214163","DOIUrl":"10.1016/j.bioadv.2024.214163","url":null,"abstract":"<div><div>Abdominal hernia repair is a common surgical procedure, involving in most cases the use of textile meshes providing a mechanical barrier to consolidate the damaged surrounding tissues and prevent the resurgence of the hernia. However, in more than half cases postoperative complications such as adhesions and infections occur at the surface of the mesh, leading to chronic pain for the patient and requiring the removal of the implant. One of the most promising strategies to reduce the risk of postoperative adhesions and infections is to add a physical barrier between the mesh and the abdominal walls. In this study, we propose a strategy to develop functional hernia meshes possessing anticoagulant and antibacterial activities depending on the side of the implant. Two bioactive polymers were synthetized: a polysulfonate (poly(2-acrylamido-2-methylpropane sulfonic acid), PAMPS) one for anticoagulant activity and a polymer bearing ternary amines (poly((2-tert-butylamino) ethyl methacrylate), PTBAEMA) for antibacterial activity. These polymers were used to produce core-sheath nanofibers thanks to coaxial electrospinning with poly(ɛ-caprolactone) (PCL) as core and the bioactive polymer as sheath. The electrospinning parameters were optimized in order to obtain defect-free nanofibrous coatings onto the mesh with improved stability in water. The core-sheath structure was investigated as well as the presence of the functional groups at the surface. The <em>in vitro</em> cytocompatibility, anticoagulant activity and antibacterial activity were evaluated and highlighted the high potential of these coatings for the simultaneous prevention of postoperative adhesions and infections.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"169 ","pages":"Article 214163"},"PeriodicalIF":5.5,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142933490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-02DOI: 10.1016/j.bioadv.2024.214173
Chee Hoe Kong , Chris Steffi , Yanli Cai , Wilson Wang
Osteoporosis, characterized by reduced bone mineral density and increased fracture risk, poses a significant health challenge, particularly for aging populations. Systemic treatments often lead to adverse side effects, emphasizing the need for localized solutions. This study introduces a 3D-printed polycaprolactone (PCL) scaffold embedded with strontium-substituted mesoporous bioactive glass nanoparticles (Sr-MBGNPs) and icariin (ICN) for the targeted regeneration of osteoporotic bone. The scaffold was characterized using scanning electron microscopy (SEM), energy-dispersive spectroscopy (EDS), ion release studies, and cellular assays, which confirmed its dual functionality in both enhancing osteoblast proliferation and differentiation and inhibiting osteoclastogenesis. The optimized Sr-MBGNP concentration ensured sustained ion release, superior hydrophilicity, and bioactivity without compromising scaffold integrity. Additionally, e-jet printing provided high precision and uniform pore sizes conducive to cellular activity. This novel scaffold platform demonstrates a promising localized treatment strategy, reducing systemic side effects while improving fixation stability. The innovative integration of Sr-MBGNPs and ICN highlights its potential to revolutionize osteoporosis therapy by promoting bone regeneration and mitigating bone resorption.
{"title":"E-jet printed polycaprolactone with strontium-substituted mesoporous bioactive glass nanoparticles for bone tissue engineering","authors":"Chee Hoe Kong , Chris Steffi , Yanli Cai , Wilson Wang","doi":"10.1016/j.bioadv.2024.214173","DOIUrl":"10.1016/j.bioadv.2024.214173","url":null,"abstract":"<div><div>Osteoporosis, characterized by reduced bone mineral density and increased fracture risk, poses a significant health challenge, particularly for aging populations. Systemic treatments often lead to adverse side effects, emphasizing the need for localized solutions. This study introduces a 3D-printed polycaprolactone (PCL) scaffold embedded with strontium-substituted mesoporous bioactive glass nanoparticles (Sr-MBGNPs) and icariin (ICN) for the targeted regeneration of osteoporotic bone. The scaffold was characterized using scanning electron microscopy (SEM), energy-dispersive spectroscopy (EDS), ion release studies, and cellular assays, which confirmed its dual functionality in both enhancing osteoblast proliferation and differentiation and inhibiting osteoclastogenesis. The optimized Sr-MBGNP concentration ensured sustained ion release, superior hydrophilicity, and bioactivity without compromising scaffold integrity. Additionally, e-jet printing provided high precision and uniform pore sizes conducive to cellular activity. This novel scaffold platform demonstrates a promising localized treatment strategy, reducing systemic side effects while improving fixation stability. The innovative integration of Sr-MBGNPs and ICN highlights its potential to revolutionize osteoporosis therapy by promoting bone regeneration and mitigating bone resorption.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"169 ","pages":"Article 214173"},"PeriodicalIF":5.5,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142928746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-02DOI: 10.1016/j.bioadv.2024.214172
Dongmin Chen , Xiaosheng Sheng , Huili Li , Qishu Jin , Ruqi Wang , Yuanzheng Qiu , Lefeng Su , Jinfeng Xu , Jiang Chang , Yumei Que , Chen Yang
The current unavailability of efficient myocardial repair therapies constitutes a significant bottleneck in the clinical management of myocardial infarction (MI). Ginsenoside Rb1 (GRb1) has emerged as a compound with potential benefits in safeguarding myocardial cells and facilitating the regeneration of myocardial tissue. However, its efficacy in treating MI-related ischemic conditions is hampered by its low bioavailability and inadequate angiogenic properties. In this study, the therapeutic potential of GRb1 is enhanced by a mesoporous basic copper carbonate (BCC) microsphere due to its excellent drug delivery capability and steady angiogenic degradation products (copper ions, Cu2+). The cell experiments revealed that GRb1 and Cu2+ could generate synergistic impacts on anti-cardiomyocyte apoptosis and endothelial cell angiogenesis, while a mouse model of MI illustrated that GRb1 loaded BCC (BCC@GRb1) could significantly enhance cardiac function, diminish the area of infarction and myocardial hypertrophy, reduce cardiomyocyte apoptosis, and augment vascularization within myocardial tissue. This investigation is pioneering in demonstrating the beneficial outcomes of combining drugs with bioactive carriers in myocardial regeneration and introduces a novel, precisely engineered drug delivery system as a potential therapeutic strategy for ischemic heart disease.
{"title":"Biodegradable copper-containing mesoporous microspheres loaded with ginsenoside Rb1 for infarcted heart repair","authors":"Dongmin Chen , Xiaosheng Sheng , Huili Li , Qishu Jin , Ruqi Wang , Yuanzheng Qiu , Lefeng Su , Jinfeng Xu , Jiang Chang , Yumei Que , Chen Yang","doi":"10.1016/j.bioadv.2024.214172","DOIUrl":"10.1016/j.bioadv.2024.214172","url":null,"abstract":"<div><div>The current unavailability of efficient myocardial repair therapies constitutes a significant bottleneck in the clinical management of myocardial infarction (MI). Ginsenoside Rb1 (GRb1) has emerged as a compound with potential benefits in safeguarding myocardial cells and facilitating the regeneration of myocardial tissue. However, its efficacy in treating MI-related ischemic conditions is hampered by its low bioavailability and inadequate angiogenic properties. In this study, the therapeutic potential of GRb1 is enhanced by a mesoporous basic copper carbonate (BCC) microsphere due to its excellent drug delivery capability and steady angiogenic degradation products (copper ions, Cu<sup>2+</sup>). The cell experiments revealed that GRb1 and Cu<sup>2+</sup> could generate synergistic impacts on anti-cardiomyocyte apoptosis and endothelial cell angiogenesis, while a mouse model of MI illustrated that GRb1 loaded BCC (BCC@GRb1) could significantly enhance cardiac function, diminish the area of infarction and myocardial hypertrophy, reduce cardiomyocyte apoptosis, and augment vascularization within myocardial tissue. This investigation is pioneering in demonstrating the beneficial outcomes of combining drugs with bioactive carriers in myocardial regeneration and introduces a novel, precisely engineered drug delivery system as a potential therapeutic strategy for ischemic heart disease.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"169 ","pages":"Article 214172"},"PeriodicalIF":5.5,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142933493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-02DOI: 10.1016/j.bioadv.2024.214170
Tina Sadat Hashemi , Satish Jaiswal , Helen O. McCarthy , Tanya J. Levingstone , Nicholas J. Dunne
Magnesium (Mg) alloys have gained significant attention as a desirable choice of biodegradable implant for use in bone repair applications, largely owing to their unique material properties. More recently, Mg and Mg-based alloys have been used as load-bearing metallic scaffolds for bone tissue engineering applications, offering promising opportunities in the field. The mechanical properties and relative density of Mg-based alloys closely approximate those of natural human bone tissue, thereby mitigating the risk of stress-shielding effects. Furthermore, the inherent biodegradability of Mg-based alloys eliminates the necessity for a second surgical procedure for the removal of the implant, a frequent requirement with conventional non-degradable implants. However, a notable challenge remains in managing the high corrosion rate of Mg and Mg-based alloys within physiological environments to ensure that they meet the necessary functional requirements. Consequently, a comprehensive analysis and understanding of the corrosion behaviour of Mg and Mg-based alloys, coupled with optimisation of their surface properties, assume pivotal significance to ensure successful clinical application. The personalized 3D printing of Mg and Mg-based alloy implants represents a paradigm shift, offering a plethora of advantages, foremost among them being the enhancement of the bone healing process facilitated by the degradable porous structure conducive to bone ingrowth. Also, the emergence of surface functionalisation techniques for Mg-based implants amalgamates the mechanical and degradation properties inherent to metals with the enhanced biofunctionality offered by these coatings. This synergy presents a highly promising avenue for using Mg-based implants as temporary orthopaedic and dental solutions. This comprehensive review provides a detailed analysis of recent advancements encompassing alloying elements, additive manufacturing processes, lattice structures and biofunctionalised coatings to tailor the corrosion resistance, mechanical properties and biocompatibility of Mg-based orthopaedic implants.
{"title":"Biofunctionalisation of porous additively manufactured magnesium-based alloys for Orthopaedic applications: A review","authors":"Tina Sadat Hashemi , Satish Jaiswal , Helen O. McCarthy , Tanya J. Levingstone , Nicholas J. Dunne","doi":"10.1016/j.bioadv.2024.214170","DOIUrl":"10.1016/j.bioadv.2024.214170","url":null,"abstract":"<div><div>Magnesium (Mg) alloys have gained significant attention as a desirable choice of biodegradable implant for use in bone repair applications, largely owing to their unique material properties. More recently, Mg and Mg-based alloys have been used as load-bearing metallic scaffolds for bone tissue engineering applications, offering promising opportunities in the field. The mechanical properties and relative density of Mg-based alloys closely approximate those of natural human bone tissue, thereby mitigating the risk of stress-shielding effects. Furthermore, the inherent biodegradability of Mg-based alloys eliminates the necessity for a second surgical procedure for the removal of the implant, a frequent requirement with conventional non-degradable implants. However, a notable challenge remains in managing the high corrosion rate of Mg and Mg-based alloys within physiological environments to ensure that they meet the necessary functional requirements. Consequently, a comprehensive analysis and understanding of the corrosion behaviour of Mg and Mg-based alloys, coupled with optimisation of their surface properties, assume pivotal significance to ensure successful clinical application. The personalized 3D printing of Mg and Mg-based alloy implants represents a paradigm shift, offering a plethora of advantages, foremost among them being the enhancement of the bone healing process facilitated by the degradable porous structure conducive to bone ingrowth. Also, the emergence of surface functionalisation techniques for Mg-based implants amalgamates the mechanical and degradation properties inherent to metals with the enhanced biofunctionality offered by these coatings. This synergy presents a highly promising avenue for using Mg-based implants as temporary orthopaedic and dental solutions. This comprehensive review provides a detailed analysis of recent advancements encompassing alloying elements, additive manufacturing processes, lattice structures and biofunctionalised coatings to tailor the corrosion resistance, mechanical properties and biocompatibility of Mg-based orthopaedic implants.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"169 ","pages":"Article 214170"},"PeriodicalIF":5.5,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142967297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-30DOI: 10.1016/j.bioadv.2024.214168
Javier Jiménez-Holguín , Daniel Lozano , Melchor Saiz-Pardo , David de Pablo , Luis Ortega , Silvia Enciso , Blanca Fernández-Tomé , Idoia Díaz-Güemes , Francisco Miguel Sánchez-Margallo , María Teresa Portolés , Daniel Arcos
Local delivery of therapeutic ions from bioactive mesoporous glasses (MBGs) is postulated as one of the most promising strategies for regenerative therapy of critical bone defects. Among these ions, Sr2+ cation has been widely considered for this purpose as part of the composition of MBGs. MBGs of chemical composition 75SiO2-25-x CaO-5P2O5-xSrO with x = 0, 2.5 and 5 (% mol) were prepared by the evaporation induced self-assembly (EISA) method. Strontium incorporation did not affect the apatite forming ability of Sr-free MBG when these bioceramics are treated with simulated body fluid (SBF). In vitro cell viability showed that proliferation of MC3T3-E1 preosteoblast is not affected by the presence of Sr2+ cations, whereas ALP activity and gene expression of Runx2, ALP and VEGF is increased as a function of Sr content. Besides, cell proliferation and VEGF expression of HUVEC cells were also increased with the Sr2+ content. In this work, we present for the first time the effects of Sr containing MBGs on bone regeneration in a large animal model (sheep), after implantation in a cavitary defect. The histomorphometrical analysis and immunohistochemistry indicate that the incorporation of Sr2+ ion greatly enhances the osteoregenerating potential of MBGs. In this sense, the measured ossification areas were 7 % and 20 % for MBG and Sr-MBG, respectively. Besides, the thickness of the newly formed trabeculae was 15 μm and 30 μm for MBG and Sr-MBG, respectively. This enhancement of Sr2+ mediated bone formation would be justified by the transient osteoclastogenesis inhibition and the osteogenesis-angiogenesis increase due to the endothelial cell proliferation and increased vascular endothelial growth factor expression.
{"title":"Bone regeneration in sheep model induced by strontium-containing mesoporous bioactive glasses","authors":"Javier Jiménez-Holguín , Daniel Lozano , Melchor Saiz-Pardo , David de Pablo , Luis Ortega , Silvia Enciso , Blanca Fernández-Tomé , Idoia Díaz-Güemes , Francisco Miguel Sánchez-Margallo , María Teresa Portolés , Daniel Arcos","doi":"10.1016/j.bioadv.2024.214168","DOIUrl":"10.1016/j.bioadv.2024.214168","url":null,"abstract":"<div><div>Local delivery of therapeutic ions from bioactive mesoporous glasses (MBGs) is postulated as one of the most promising strategies for regenerative therapy of critical bone defects. Among these ions, Sr<sup>2+</sup> cation has been widely considered for this purpose as part of the composition of MBGs. MBGs of chemical composition 75SiO<sub>2</sub>-25-x CaO-5P<sub>2</sub>O<sub>5</sub>-xSrO with x = 0, 2.5 and 5 (% mol) were prepared by the evaporation induced self-assembly (EISA) method. Strontium incorporation did not affect the apatite forming ability of Sr-free MBG when these bioceramics are treated with simulated body fluid (SBF). <em>In vitro</em> cell viability showed that proliferation of MC3T3-E1 preosteoblast is not affected by the presence of Sr<sup>2+</sup> cations, whereas ALP activity and gene expression of Runx2, ALP and VEGF is increased as a function of Sr content. Besides, cell proliferation and VEGF expression of HUVEC cells were also increased with the Sr<sup>2+</sup> content. In this work, we present for the first time the effects of Sr containing MBGs on bone regeneration in a large animal model (sheep), after implantation in a cavitary defect. The histomorphometrical analysis and immunohistochemistry indicate that the incorporation of Sr<sup>2+</sup> ion greatly enhances the osteoregenerating potential of MBGs. In this sense, the measured ossification areas were 7 % and 20 % for MBG and Sr-MBG, respectively. Besides, the thickness of the newly formed trabeculae was 15 μm and 30 μm for MBG and Sr-MBG, respectively. This enhancement of Sr<sup>2+</sup> mediated bone formation would be justified by the transient osteoclastogenesis inhibition and the osteogenesis-angiogenesis increase due to the endothelial cell proliferation and increased vascular endothelial growth factor expression.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"169 ","pages":"Article 214168"},"PeriodicalIF":5.5,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142933472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-30DOI: 10.1016/j.bioadv.2024.214169
Ruijia Yan , Shu Chen , Bixue Wang, Changsheng Liu, Xi Chen
Spinal cord injury (SCI) results in electrophysiological and behavioral dysfunction. Electrical stimulation (ES) is considered to be an effective treatment for mild SCI; however, ES is not applicable to severe SCI due to the disruption of electrical conduction caused by tissue defects. Therefore, the use of conductive materials to fill the defects and restore electrical conduction in the spinal cord is a promising therapeutic strategy. In this study, we used ultrasound to composite conductive reduced graphene oxide (rGO) and magnetic Fe3O4 nanoparticles and encapsulated them into gelatin methacryloyl (GelMA) along with decellularized extracellular matrix (dECM) to form a conductive composite hydrogel, rGO/Fe3O4/dECM@GelMA. The rGO/Fe3O4 complexes were able to orientate themselves in the hydrogel with a magnetic field, conferring an orientated electrical conduction function to the hydrogel. The implantation of this composite hydrogel re-established the electrical conduction in the damaged spinal cord and synergized with ES to promote the regeneration of neurons and myelinated axons at the injury site, resulting in the restoration of electrophysiological function of the spinal cord and motor function of the hind limbs of mice. Our study combines a conductive tissue-engineered scaffold with ES therapy to improve the efficacy of ES in severe spinal cord injuries and promote the restoration of spinal cord function.
{"title":"Magnetic field-oriented conductive decellularized extracellular matrix hydrogel synergizes with electrical stimulation to promote spinal cord injury repair and electrophysiological function restoration","authors":"Ruijia Yan , Shu Chen , Bixue Wang, Changsheng Liu, Xi Chen","doi":"10.1016/j.bioadv.2024.214169","DOIUrl":"10.1016/j.bioadv.2024.214169","url":null,"abstract":"<div><div>Spinal cord injury (SCI) results in electrophysiological and behavioral dysfunction. Electrical stimulation (ES) is considered to be an effective treatment for mild SCI; however, ES is not applicable to severe SCI due to the disruption of electrical conduction caused by tissue defects. Therefore, the use of conductive materials to fill the defects and restore electrical conduction in the spinal cord is a promising therapeutic strategy. In this study, we used ultrasound to composite conductive reduced graphene oxide (rGO) and magnetic Fe<sub>3</sub>O<sub>4</sub> nanoparticles and encapsulated them into gelatin methacryloyl (GelMA) along with decellularized extracellular matrix (dECM) to form a conductive composite hydrogel, rGO/Fe<sub>3</sub>O<sub>4</sub>/dECM@GelMA. The rGO/Fe<sub>3</sub>O<sub>4</sub> complexes were able to orientate themselves in the hydrogel with a magnetic field, conferring an orientated electrical conduction function to the hydrogel. The implantation of this composite hydrogel re-established the electrical conduction in the damaged spinal cord and synergized with ES to promote the regeneration of neurons and myelinated axons at the injury site, resulting in the restoration of electrophysiological function of the spinal cord and motor function of the hind limbs of mice. Our study combines a conductive tissue-engineered scaffold with ES therapy to improve the efficacy of ES in severe spinal cord injuries and promote the restoration of spinal cord function.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"169 ","pages":"Article 214169"},"PeriodicalIF":5.5,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142928745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-29DOI: 10.1016/j.bioadv.2024.214162
Xiao Xu , Jiao Sun
There are two bottlenecks in the treatment of TMJOA (temporomandibular joint osteoarthritis): ① lacking of easy-to-use repairing materials for damaged condylar cartilage; ② local inflammation interfering with in situ regeneration. In response to them, we constructed a biomimetic tilapia type I gelatin/hyaluronic acid (TGI/HA) hydrogel in this paper. It was endowed with the capability to immunoregulate mircoenvironment and concurrently induce regeneration in multiple ways. It not only reduced excretion of ECM-degrading enzymes and inflammatory factors, therefore reversing local inflammation, but also created microenvironment conducive to reparation by acting upon macrophages and T cells. In in vivo experiments, the TGI/HA hydrogel effectively restored the damaged cartilage on rat condyle, suggesting it had potential in clinical application.
{"title":"A mini-invasive injectable hydrogel for temporomandibular joint osteoarthritis: Its pleiotropic effects and multiple pathways in cartilage regeneration","authors":"Xiao Xu , Jiao Sun","doi":"10.1016/j.bioadv.2024.214162","DOIUrl":"10.1016/j.bioadv.2024.214162","url":null,"abstract":"<div><div>There are two bottlenecks in the treatment of TMJOA (temporomandibular joint osteoarthritis): ① lacking of easy-to-use repairing materials for damaged condylar cartilage; ② local inflammation interfering with <em>in situ</em> regeneration. In response to them, we constructed a biomimetic tilapia type I gelatin/hyaluronic acid (TGI/HA) hydrogel in this paper. It was endowed with the capability to immunoregulate mircoenvironment and concurrently induce regeneration in multiple ways. It not only reduced excretion of ECM-degrading enzymes and inflammatory factors, therefore reversing local inflammation, but also created microenvironment conducive to reparation by acting upon macrophages and T cells. In <em>in vivo</em> experiments, the TGI/HA hydrogel effectively restored the damaged cartilage on rat condyle, suggesting it had potential in clinical application.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"169 ","pages":"Article 214162"},"PeriodicalIF":5.5,"publicationDate":"2024-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142916201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-29DOI: 10.1016/j.bioadv.2024.214166
Jacek K. Wychowaniec , Ezgi Irem Bektas , Andrea J. Vernengo , Marcia Muerner , Marielle Airoldi , Paul Sean Tipay , Jiranuwat Sapudom , Jeremy Teo , David Eglin , Matteo D'Este
The immunomodulatory properties of hyaluronan and its derivatives are key to their use in medicine and tissue engineering. In this work we evaluated the capability of soluble tyramine-modified hyaluronan (THA) synthesized from hyaluronan of two molecular weights (low Mw = 280 kDa and high Mw = 1640 kDa) for polarization of THP-1 and peripheral blood mononuclear cells (PBMCs)-derived macrophages (MΦs). We demonstrate the polarization effects of the supplemented THA by flow cytometry and bead-based multiplex immunoassay for the THP-1 derived MΦs and by semi-automated image analysis from confocal microscopy, immunofluorescent staining utilizing CD68 and CD206 surface markers, RT-qPCR gene expression analysis, as well as using the enzyme-linked immunosorbent assay (ELISA) for PBMCs-derived MΦs. Our data indicate that supplementation with LMW THA drives changes in THP-1 derived MΦs towards a pro-inflammatory M1-like phenotype, whereas supplementation with the HMW THA leads to a more mixed profile with some features of both M1 and M2 phenotypes, suggesting either a heterogeneous population or a transitional state. For cells directly sourced from human patients, PMBCs-derived MΦs, results exhibit a higher degree of variability, pointing out a differential regulation of factors including IL-10 and CD206 between the two cell sources. While human primary cells add to the clinical relevance, donor diversity introduces wider variability in the dataset, preventing drawing strong conclusions. Nevertheless, the MΦs profiles observed in THP-1 derived cells for treatments with LMW and HMW THA are generally consistent with what might be expected for the treatment with non-modified hyaluronans of respective molecular weights, confirming the known association holds true for the chemically tyramine-modified hyaluronan. We stipulate that these responses will provide basis for more accurate in vivo representation and translational immunomodulatory guidance for the use of THA-based biomaterials to a wider biomaterials and tissue engineering communities.
{"title":"Effect of molecular weight of tyramine-modified hyaluronan on polarization state of THP-1 and peripheral blood mononuclear cells-derived macrophages","authors":"Jacek K. Wychowaniec , Ezgi Irem Bektas , Andrea J. Vernengo , Marcia Muerner , Marielle Airoldi , Paul Sean Tipay , Jiranuwat Sapudom , Jeremy Teo , David Eglin , Matteo D'Este","doi":"10.1016/j.bioadv.2024.214166","DOIUrl":"10.1016/j.bioadv.2024.214166","url":null,"abstract":"<div><div>The immunomodulatory properties of hyaluronan and its derivatives are key to their use in medicine and tissue engineering. In this work we evaluated the capability of soluble tyramine-modified hyaluronan (THA) synthesized from hyaluronan of two molecular weights (low M<sub>w</sub> = 280 kDa and high M<sub>w</sub> = 1640 kDa) for polarization of THP-1 and peripheral blood mononuclear cells (PBMCs)-derived macrophages (MΦs). We demonstrate the polarization effects of the supplemented THA by flow cytometry and bead-based multiplex immunoassay for the THP-1 derived MΦs and by semi-automated image analysis from confocal microscopy, immunofluorescent staining utilizing CD68 and CD206 surface markers, RT-qPCR gene expression analysis, as well as using the enzyme-linked immunosorbent assay (ELISA) for PBMCs-derived MΦs. Our data indicate that supplementation with LMW THA drives changes in THP-1 derived MΦs towards a pro-inflammatory M1-like phenotype, whereas supplementation with the HMW THA leads to a more mixed profile with some features of both M1 and M2 phenotypes, suggesting either a heterogeneous population or a transitional state. For cells directly sourced from human patients, PMBCs-derived MΦs, results exhibit a higher degree of variability, pointing out a differential regulation of factors including IL-10 and CD206 between the two cell sources. While human primary cells add to the clinical relevance, donor diversity introduces wider variability in the dataset, preventing drawing strong conclusions. Nevertheless, the MΦs profiles observed in THP-1 derived cells for treatments with LMW and HMW THA are generally consistent with what might be expected for the treatment with non-modified hyaluronans of respective molecular weights, confirming the known association holds true for the chemically tyramine-modified hyaluronan. We stipulate that these responses will provide basis for more accurate <em>in vivo</em> representation and translational immunomodulatory guidance for the use of THA-based biomaterials to a wider biomaterials and tissue engineering communities.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"169 ","pages":"Article 214166"},"PeriodicalIF":5.5,"publicationDate":"2024-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143015365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-29DOI: 10.1016/j.bioadv.2024.214164
Xiaohan Sun , Nanxin Zhang , Longhui Chen , Yuchao Lai , Shasha Yang , Qiang Li , Yunquan Zheng , Li Chen , Xianai Shi , Jianmin Yang
In anterior cruciate ligament (ACL) repair methods, the continuous enzymatic erosion of synovial fluid can impede healing and potentially lead to repair failure, as well as exacerbate articular cartilage wear, resulting in joint degeneration. Inspired by the blood clot during medial collateral ligament healing, we developed a composite scaffold comprising collagen (1 %, w/v) and polyvinyl alcohol (5 %, w/v) combined with platelet-rich plasma (PRP). The composite scaffold provides a protective barrier against synovial erosion for the ruptured ACL, while simultaneously facilitating tissue repair, thereby enhancing the efficacy of ACL repair techniques. The composite scaffold is primarily formed through hydrogen bonding between molecular chains and physical cross-linking of microcrystalline regions using a simple cyclic freeze-thaw method, resulting in improved mechanical properties and an extended degradation period. The maximum tensile fracture load of the composite scaffold reached 5.99 ± 0.30 N. The incorporation of PRP facilitates cell migration, proliferation, and blood vessel growth by enabling slow release of various growth factors. In vivo results demonstrate that this composite scaffold promotes rabbit hindlimb rupture ACL healing by stimulating fibroblast proliferation, collagen deposition, microvascular formation, and proprioceptor generation. Furthermore, it effectively reduces meniscus and cartilage wear while mitigating bone arthritis and joint degenerative diseases. Overall, our proposed composite scaffold holds great promise as a candidate for ACL healing.
{"title":"Collagen/polyvinyl alcohol scaffolds combined with platelet-rich plasma to enhance anterior cruciate ligament repair","authors":"Xiaohan Sun , Nanxin Zhang , Longhui Chen , Yuchao Lai , Shasha Yang , Qiang Li , Yunquan Zheng , Li Chen , Xianai Shi , Jianmin Yang","doi":"10.1016/j.bioadv.2024.214164","DOIUrl":"10.1016/j.bioadv.2024.214164","url":null,"abstract":"<div><div>In anterior cruciate ligament (ACL) repair methods, the continuous enzymatic erosion of synovial fluid can impede healing and potentially lead to repair failure, as well as exacerbate articular cartilage wear, resulting in joint degeneration. Inspired by the blood clot during medial collateral ligament healing, we developed a composite scaffold comprising collagen (1 %, w/v) and polyvinyl alcohol (5 %, w/v) combined with platelet-rich plasma (PRP). The composite scaffold provides a protective barrier against synovial erosion for the ruptured ACL, while simultaneously facilitating tissue repair, thereby enhancing the efficacy of ACL repair techniques. The composite scaffold is primarily formed through hydrogen bonding between molecular chains and physical cross-linking of microcrystalline regions using a simple cyclic freeze-thaw method, resulting in improved mechanical properties and an extended degradation period. The maximum tensile fracture load of the composite scaffold reached 5.99 ± 0.30 N. The incorporation of PRP facilitates cell migration, proliferation, and blood vessel growth by enabling slow release of various growth factors. <em>In vivo</em> results demonstrate that this composite scaffold promotes rabbit hindlimb rupture ACL healing by stimulating fibroblast proliferation, collagen deposition, microvascular formation, and proprioceptor generation. Furthermore, it effectively reduces meniscus and cartilage wear while mitigating bone arthritis and joint degenerative diseases. Overall, our proposed composite scaffold holds great promise as a candidate for ACL healing.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"169 ","pages":"Article 214164"},"PeriodicalIF":5.5,"publicationDate":"2024-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142933487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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