Materials Science & Engineering C-Materials for Biological Applications最新文献_第6页

Corrigendum to “Evaluating antimicrobial efficacy in medical devices: The critical role of simulating in use test conditions” [Biomater. Adv. 172 (2025), 214241] “评估医疗器械的抗菌功效：模拟使用试验条件的关键作用”的勘误表[Biomater]。[j].生物工程学报，2003,24(4):387 - 391。

IF 6 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications

Pub Date : 2026-01-23 DOI: 10.1016/j.bioadv.2026.214729

Sofia Wareham-Mathiassen , Mohammed Nateqi , Sai Achyuth Badrinarayanan , Vera Pinto Glenting , Mette Bjergaard Dragheim , Arendse Ross Agner , Tina Secher Rasmussen , Rahul Singh , Winnie Edith Svendsen , Lene Bay , Lars Jelsbak , Henrik Bengtsson , Thomas Bjarnsholt

引用次数: 0

Thermosensitive nanogel-based oxaliplatin delivery system for synergistic intratumoral radiofrequency chemotherapy 基于热敏纳米凝胶的肿瘤内射频化疗奥沙利铂递送系统

IF 6 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications

Pub Date : 2026-01-22 DOI: 10.1016/j.bioadv.2026.214726

Ling Li , Anna Liu , Cai Wang , Ling Zhang , Houqiang Yu , Han Li , Hongfu Zhou

To achieve sustained release and prolonged tumor retention of oxaliplatin (OXA), a thermosensitive OXA-loaded poly(N-isopropyl acrylamide-co-acrylic acid) nanogel (PAAs) was developed via a synergistic mixing-stirring method. The formulation, consisting of 0.8 mg/mL OXA thoroughly dispersed in 8% poly(N-isopropyl acrylamide-co-acrylic acid) nanogel (PNAs), exhibited favorable radiofrequency responsiveness, thermosensitivity, and controlled-release properties, enabling continuous OXA release for up to five days. The sol–gel phase transition behavior of the thermosensitive PAAs nanogel was characterized using the vial-inversion method and rheological analysis. Platinum content analysis revealed enhanced tumor retention of the PAAs nanogel compared with free OXA, as evidenced by significantly higher platinum levels in tumors treated with the nanogel formulation. In vivo antitumor efficacy evaluation demonstrated that a single administration of the PAAs nanogel resulted in sustained tumor regression, reducing the relative tumor volume to 0.81 ± 0.06 times the initial volume within 14 days. In contrast, treatment with an equivalent dose of free OXA, PNAs alone, or normal saline led to rapid tumor progression, with tumor volumes increasing to 3.22 ± 0.65, 7.01 ± 0.47, and 10.07 ± 1.57 times the initial volume, respectively. Preliminary biocompatibility assessment indicated that the incorporation of OXA into PNAs within the nanogel significantly alleviated the toxic side effects associated with free OXA. These findings underscore the considerable potential of the PAAs nanogel as a versatile strategy to enhance the antitumor efficacy of platinum-based drugs while mitigating their systemic toxicity and size-related limitations. This system therefore represents a promising candidate for further development as a novel nanomedicine for localized chemotherapy.

为了实现奥沙利铂（OXA）的缓释和肿瘤滞留，通过协同混合-搅拌方法制备了一种热敏负载OXA的聚（n -异丙基丙烯酰胺-共丙烯酸）纳米凝胶（PAAs）。该配方由0.8 mg/mL OXA完全分散在8%聚（n -异丙基丙烯酰胺-共丙烯酸）纳米凝胶（PNAs）组成，具有良好的射频响应性、热敏性和控释特性，OXA可连续释放长达5天。采用小瓶倒置法和流变学分析表征了热敏PAAs纳米凝胶的溶胶-凝胶相变行为。铂含量分析显示，与游离OXA相比，PAAs纳米凝胶增强了肿瘤保留率，用纳米凝胶治疗的肿瘤中铂含量显著提高。体内抗肿瘤疗效评估表明，单次给药PAAs纳米凝胶可使肿瘤持续消退，在14天内使肿瘤相对体积减少到初始体积的0.81±0.06倍。相反，使用等剂量的游离OXA、PNAs或生理盐水治疗可导致肿瘤快速进展，肿瘤体积分别增加到初始体积的3.22±0.65、7.01±0.47和10.07±1.57倍。初步的生物相容性评估表明，将OXA掺入纳米凝胶内的PNAs可显著减轻游离OXA相关的毒副作用。这些发现强调了PAAs纳米凝胶作为一种通用策略的巨大潜力，可以增强铂类药物的抗肿瘤疗效，同时减轻其全身毒性和尺寸相关的局限性。因此，该系统代表了一种有希望进一步发展的新型纳米药物，用于局部化疗。

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引用次数: 0

Lysophosphatidic acid-induced upregulation of exosomal miR-221-3p from corneal stromal cells promotes corneal endothelial healing 溶血磷脂酸诱导的角膜基质细胞外泌体miR-221-3p上调可促进角膜内皮细胞愈合。

IF 6 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications

Pub Date : 2026-01-22 DOI: 10.1016/j.bioadv.2026.214719

Hung-Chi Chen , Yi-Jen Hsueh , Yaa-Jyuhn James Meir , Jui-Yang Lai , Chieh-Cheng Huang , Tsai-Te Lu , Chao-Min Cheng , Song-Shu Lin , David Hui-Kang Ma , Wei-Chi Wu

Corneal transparency maintenance relies on the water-pumping function of the corneal endothelium. Currently, corneal transplantation remains the only available treatment for corneal endothelial dysfunction, therefore, the development of alternative therapies is critical due to the global shortage of donor corneas. In our previous study, we confirmed that corneal stromal cells (CSCs) secretion can promote corneal endothelial cells (CEnCs) proliferation. This effect can be enhanced by treatment with lysophosphatidic acid (LPA), a bioactive phospholipid. Nevertheless, the components involved in CSC secretion remain to be elucidated. In this study, we investigated the therapeutic potential of CSC-derived exosomes and exosomal microRNAs (miRNAs) for enhancing CEnCs proliferation and corneal endothelial healing. CSC exosomes were characterized via nanoparticle tracking (NTA), transmission electron microscopy (TEM), and immunoassays. The miRNA expression profiles of CSC exosomes were identified via RNA sequencing, revealing a total of 767 distinct miRNAs. The proliferative effects of CSC exosomes and exosomal miR-221-3p were increased by LPA. Ectopic expression of miR-221-3p further increased CEnC proliferation and suppressed the expression of the CDK inhibitor p27^Kip1. The therapeutic efficacy was evaluated using a transcorneal freezing rabbit model, where intrastromal injection of CSC exosomes or ectopic expression of miR-221-3p significantly ameliorated corneal endothelial damage, as supported by improved in vivo corneal recovery, including restoration of corneal thickness, and re-establishment of a hexagonal morphology in the corneal endothelium. Our findings suggest that CSC exosomes and miR-221-3p represent potentially promising cell-free therapies for treating corneal endothelial diseases, highlighting an innovative approach to improving corneal regeneration.

角膜透明的维持依赖于角膜内皮的抽水功能。目前，角膜移植仍然是治疗角膜内皮功能障碍的唯一方法，因此，由于全球供体角膜短缺，开发替代疗法至关重要。在我们之前的研究中，我们证实了角膜基质细胞（CSCs）的分泌可以促进角膜内皮细胞（CEnCs）的增殖。这种效果可以通过溶血磷脂酸（LPA）治疗来增强，这是一种生物活性磷脂。然而，参与CSC分泌的成分仍有待阐明。在这项研究中，我们研究了csc来源的外泌体和外泌体microRNAs （miRNAs）在促进ccs增殖和角膜内皮愈合方面的治疗潜力。通过纳米颗粒跟踪（NTA）、透射电子显微镜（TEM）和免疫分析对CSC外泌体进行了表征。通过RNA测序鉴定CSC外泌体的miRNA表达谱，共发现767种不同的miRNA。LPA增加了CSC外泌体和外泌体miR-221-3p的增殖作用。miR-221-3p的异位表达进一步增加了CEnC的增殖，抑制了CDK抑制剂p27Kip1的表达。使用经角膜冷冻兔模型评估治疗效果，其中，在角膜内注射CSC外泌体或异位表达miR-221-3p可显着改善角膜内皮损伤，这得到了体内角膜恢复的支持，包括角膜厚度的恢复，以及角膜内皮六边形形态的重建。我们的研究结果表明，CSC外泌体和miR-221-3p代表了治疗角膜内皮疾病的潜在有前途的无细胞疗法，突出了改善角膜再生的创新方法。

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引用次数: 0

Multilayered electrospun membranes incorporating microspheres embedded nanofibers for enhanced wound healing 多层电纺丝膜结合微球嵌入纳米纤维，促进伤口愈合

IF 6 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications

Pub Date : 2026-01-21 DOI: 10.1016/j.bioadv.2026.214727

Fen Ao , Wen Shen , Xuemei Ge , Yan Zheng

Multilayer membranes that coordinate the release of different drugs to match the various stages of wound healing while managing excessive tissue exudate represent a promising therapeutic approach. However, achieving precise control over the dual-drug release and effective absorption of excess tissue exudate remains a significant clinical challenge. This study employed a “nano-in-nano” microsphere-embedded fiber strategy to construct a multilayered dual drug delivery system (ML-DDS) via sequential electrospinning. The system comprises three layers: an outer fiber layer loaded with the antibacterial drug amikacin (Am), an intermediate layer of microspheres loaded with the anti-inflammatory drug quercetin (Qu) embedded within the fibers and an inner hydrophobic ethyl cellulose layer. Structural characterization showed that the intermediate layer had a diameter of approximately 500 nm with embedded microspheres predominantly ranging from 2.5 to 3.5 μm, while the inner EC fibers measured about 150 nm in diameter. The system leverages interfacial capillary forces to transport exudate from the hydrophobic layer to the drug-carrying layer. The water contact angles of the innermost hydrophobic layer and the outermost hydrophilic layer decrease to 0° within 40 s and 60 s, respectively. Drug release channels were formed after the ethyl cellulose layer absorbs water and swells. The release time of Am extended from 120 min in the single layer to 24 h in the ML-DDS. Both Qu and Am were continuously released in an amorphous form within 24 h through matrix erosion. ML-DDS up-regulated the expression of antioxidant-related metabolites, maintained mitochondrial function and promoted wound healing with a higher healing rate at day 14 compared to the control, along with enhanced collagen deposition, up-regulated CD31 expression, and reduced COX-2 levels. This study demonstrates that ML-DDS effectively integrates multilayered exudate management with controlled dual-drug delivery, providing a novel treatment strategy for skin wounds with excessive tissue exudate.

多层膜可以协调不同药物的释放，以适应伤口愈合的各个阶段，同时控制过多的组织渗出，这是一种很有前途的治疗方法。然而，实现精确控制双药释放和有效吸收多余的组织渗出液仍然是一个重大的临床挑战。本研究采用“纳米中纳米”微球嵌入纤维策略，通过序贯静电纺丝构建多层双重给药系统（ML-DDS）。该系统包括三层：外层纤维层装载抗菌药物阿米卡星（Am），中间层微球层装载抗炎药物槲皮素（Qu）嵌入纤维中，以及内部疏水乙基纤维素层。结构表征表明，中间层直径约为500 nm，嵌入的微球主要分布在2.5 ~ 3.5 μm之间，而内层的EC纤维直径约为150 nm。该系统利用界面毛细管力将渗出液从疏水层输送到载药层。最内层疏水层和最外层亲水层的水接触角分别在40 s和60 s内减小到0°。乙基纤维素层吸水膨胀后形成药物释放通道。Am的释放时间由单层的120 min延长至ML-DDS的24 h。在24 h内，Qu和Am均通过基质侵蚀以无定形形式连续释放。ML-DDS上调抗氧化相关代谢物的表达，维持线粒体功能，促进伤口愈合，与对照组相比，伤口愈合第14天的愈合率更高，同时胶原沉积增强，CD31表达上调，COX-2水平降低。本研究表明，ML-DDS有效地将多层渗出液管理与可控的双药递送相结合，为组织渗出过多的皮肤伤口提供了一种新的治疗策略。

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引用次数: 0

Surface microstructure of PEEK scaffolds regulates osteogenic differentiation via the βPIX–RAC1–NOX1 pathway PEEK支架的表面微观结构通过βPIX-RAC1-NOX1通路调控成骨分化

IF 6 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications

Pub Date : 2026-01-21 DOI: 10.1016/j.bioadv.2026.214718

Zijian Yang , Bowen Zhang , Yihao Liu , Kaiting Chen , Qing Zhang , Changning Sun , Ling Wang , Dichen Li , Qingchu Li , Huiyu Zhao

Polyetheretherketone (PEEK) is widely applied in orthopedic implants, yet its intrinsic surface bioinertness limits stable osseointegration. Although a nature solution is modifying its surface to enhance cellular adhesion, other pathways related to osseointegration also hold significant value. Here, we combined fused deposition modeling (FDM) 3D printing with a two-step poly (acrylic acid)–ethylenediamine (PAA–EDA) grafting method to engineer porous PEEK scaffolds with bioactive surface microstructures. Beyond improving hydrophilicity to optimize cellular adhesion, the modified surface activated the βPIX-mediated signaling cascade, which suppressed ITGB1–RAC1–NOX1 activity, potentially delaying bone mesenchymal stem cell (BMSC) senescence and promoting osteogenic differentiation. In vivo implantation further validated that the modified scaffolds promoted bone formation and integration. Together, this work highlights a new pathway on osseointegration for PEEK surface engineering, revealing the potential of βPIX-mediated regulation as a new direction for durable bone–implant integration.

聚醚醚酮（PEEK）广泛应用于骨科植入物中，但其固有的表面生物惰性限制了其稳定的骨整合。虽然自然解决方案是修改其表面以增强细胞粘附，但与骨整合相关的其他途径也具有重要价值。在这里，我们将熔融沉积建模（FDM） 3D打印与两步聚丙烯酸-乙二胺（PAA-EDA）接枝方法相结合，以设计具有生物活性表面微结构的多孔PEEK支架。除了改善亲水性以优化细胞粘附外，修饰后的表面还激活了β pix介导的信号级联，从而抑制ITGB1-RAC1-NOX1活性，可能延缓骨间充质干细胞（bone mesenchymal stem cell， BMSC）衰老并促进成骨分化。体内植入进一步验证了改性支架促进骨形成和整合。总之，这项工作强调了PEEK表面工程的骨整合新途径，揭示了β pix介导的调节作为持久骨植入物整合的新方向的潜力。

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引用次数: 0

In-situ engineering of a covalent organic framework-based biomimetic nanoplatform for multi-target therapy of Alzheimer’s disease 基于共价有机框架的多靶点治疗阿尔茨海默病仿生纳米平台的原位工程研究

IF 6 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications

Pub Date : 2026-01-21 DOI: 10.1016/j.bioadv.2026.214728

Yujiao Yan , Yutong Chen , Fengmei Yang , Ruixin Zhao , Die Tian , Liran Deng , Meng Xie

To address the complex pathology of Alzheimer’s disease (AD), including abnormal amyloid-β (Aβ) aggregation, metal ion homeostasis disruption and oxidative stress, we developed an integrated multifunctional nanoplatform. This platform leveraged a covalent organic framework (TD-COF) with intrinsic capabilities for Cu²⁺ chelation and Aβ inhibition as the carrier. Through in-situ engineering, ultrafine palladium nanoparticles (PdNPs) were anchored to construct a stable, functionally integrated core (Pd-COF). However, due to limitations of nanomaterials such as short half-life and poor brain targeting, we further employed red blood cell (RBC) membrane for biomimetic modification, yielding the final platform Pd-COF-RBC. In vitro experiments demonstrated that Pd-COF-RBC concurrently achieved Cu²⁺ chelation, Aβ fibrillation inhibition and reactive oxygen species (ROS) scavenging. Notably, the design of Pd-COF also regulated the size and dispersibility of PdNPs, enhancing catalase-like (CAT) activity by 34.7%. In Aβ-induced cellular models, the material effectively alleviated oxidative stress and mitochondrial dysfunction, increasing cell survival by over 78.4%. Further experiments confirmed that Pd-COF-RBC modified with RBC membrane possessed good biocompatibility, long circulation property and brain accumulation capacity. Based on these findings, we evaluated its therapeutic potential in the transgenic AD C. elegans model. The results demonstrated the motor and cognitive functions of the worms were markedly restored, with the average paralysis time prolonged by approximately 37.3% and the chemotactic index recovering to near wild-type levels. Thus, the study has promise for providing experimental evidence for multi-target intervention against the complex pathological network of AD via an integrated strategy of in situ engineering and biomimetic modification.

为了解决阿尔茨海默病（AD）的复杂病理，包括异常淀粉样蛋白-β (Aβ)聚集，金属离子稳态破坏和氧化应激，我们开发了一个集成的多功能纳米平台。该平台利用共价有机框架（TD-COF）作为载体，具有Cu2+螯合和a β抑制的内在能力。通过原位工程，将超细钯纳米颗粒（PdNPs）锚定在一个稳定的、功能集成的核心（Pd-COF）上。然而，由于纳米材料的半衰期短和脑靶向性差等局限性，我们进一步采用红细胞（RBC）膜进行仿生修饰，最终得到了Pd-COF-RBC平台。体外实验表明，Pd-COF-RBC同时具有Cu2+螯合、抑制Aβ纤颤和清除活性氧（ROS）的作用。值得注意的是，Pd-COF的设计还调节了PdNPs的大小和分散性，使过氧化氢酶样（CAT）活性提高了34.7%。在a β诱导的细胞模型中，该材料可有效缓解氧化应激和线粒体功能障碍，使细胞存活率提高78.4%以上。进一步实验证实，经红细胞膜修饰的Pd-COF-RBC具有良好的生物相容性、长循环性能和脑蓄积能力。基于这些发现，我们评估了其在转基因秀丽隐杆线虫模型中的治疗潜力。结果表明，运动和认知功能明显恢复，平均麻痹时间延长约37.3%，趋化指数恢复到接近野生型的水平。因此，该研究有望为通过原位工程和仿生修饰的综合策略对AD复杂的病理网络进行多靶点干预提供实验证据。

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引用次数: 0

Multifunctional conductive hydrogel integrating exosome delivery and electrical stimulation for enhanced diabetic wound healing 集成外泌体递送和电刺激的多功能导电水凝胶促进糖尿病伤口愈合

IF 6 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications

Pub Date : 2026-01-20 DOI: 10.1016/j.bioadv.2026.214716

Weibin Wang , Yufeng Huang , Xuehui Chen , Yuxiang Lei , Yunquan Zheng , Xianai Shi , Jianmin Yang

Diabetic wound healing is severely hampered by persistent inflammation, vascular dysfunction, infection risk, and oxidative stress. To overcome these challenges, we developed a multifunctional conductive hydrogel platform (Gel@Exo-ES). This system integrates exosomes derived from adipose-derived mesenchymal stem cells into a dynamic hydrogel network formed by cross-linking quaternized chitosan-polyaniline (QCS-PANI) with oxidized dextran (ODex). The resulting hydrogel exhibits good injectability, pH-responsive degradability, high antibacterial activity and conductivity. When combined with electrical stimulation, the Gel@Exo-ES significantly enhanced the proliferation, migration, and differentiation of fibroblasts (NIH-3T3), endothelial cells (HUVECs), and macrophages (Raw 264.7) in vitro. This synergy is attributed to the biocompatible hydrogel matrix, electrical stimulation-activated pro-healing signaling, and exosome-mediated bioactive cue delivery. In a diabetic rat model, the Gel@Exo-ES markedly accelerated wound closure by recruiting macrophages, upregulating IL-10 to drive M2 polarization, and thereby alleviating inflammation. The treatment concurrently enhanced re-epithelialization, collagen deposition, and angiogenesis. These findings demonstrate that the combined strategy of exosome-loaded conductive hydrogel and electrical stimulation presents a highly promising therapeutic platform for diabetic wound repair.

糖尿病伤口愈合受到持续炎症、血管功能障碍、感染风险和氧化应激的严重阻碍。为了克服这些挑战，我们开发了一种多功能导电水凝胶平台（Gel@Exo-ES）。该系统将来自脂肪来源的间充质干细胞的外泌体整合到一个由季铵盐化壳聚糖聚苯胺（QCS-PANI）和氧化葡聚糖（ODex）交联形成的动态水凝胶网络中。所得水凝胶具有良好的注射性、ph响应降解性、高抗菌活性和导电性。当结合电刺激时，Gel@Exo-ES显著增强了体外成纤维细胞（NIH-3T3）、内皮细胞（HUVECs）和巨噬细胞（Raw 264.7）的增殖、迁移和分化。这种协同作用归因于生物相容性水凝胶基质、电刺激激活的促愈合信号和外泌体介导的生物活性线索传递。在糖尿病大鼠模型中，Gel@Exo-ES通过招募巨噬细胞，上调IL-10驱动M2极化，从而显著加速伤口愈合，从而减轻炎症。治疗同时增强了再上皮化、胶原沉积和血管生成。这些发现表明，负载外泌体的导电水凝胶与电刺激相结合的策略为糖尿病伤口修复提供了一个非常有前途的治疗平台。

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引用次数: 0

Nanotechnology-driven drug delivery systems for breast cancer: A review 纳米技术驱动的乳腺癌药物传递系统：综述

IF 6 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications

Pub Date : 2026-01-19 DOI: 10.1016/j.bioadv.2026.214725

Tao Wang , Yuxuan Chen , Xuyang Zhang , Yuejie Dou , Ying Chen , Maobin Xie , Antonella Motta , Zhaozhu Zheng , Xiaoqin Wang , Xiaying Kuang , Zhifen Han , Gang Li

Breast cancer (BC) remains a global health challenge, with treatment limitations from systemic toxicity, poor drug bioavailability, and multidrug resistance (MDR). Recent advancements in nanotechnology have revolutionized drug delivery systems (DDS), offering targeted, controlled, and synergistic therapeutic strategies. This review explores cutting-edge micro- and nanocarriers, including liposomes (Lips), nanoparticles (NPs), metal-organic frameworks (MOFs), exosomes and nanofibers (NFs)-designed to optimize the delivery of chemotherapeutic agents. Key design parameters are critically analyzed for their roles in optimizing drug encapsulation, tumor specificity, and biocompatibility. We highlight innovations in carrier engineering that enable biological modulation. Furthermore, co-delivery systems combining chemotherapy drugs with siRNA, P-glycoprotein inhibitors, or photothermal agents demonstrate remarkable success in reversing MDR in vitro and in vivo. Despite progress, challenges such as tumor heterogeneity, long-term carrier safety, and cost-effectiveness require further investigation. This review underscores the transformative potential of nanotechnology-driven DDS in precision oncology, paving the way for next-generation therapies to combat breast BC.

乳腺癌（BC）仍然是一个全球性的健康挑战，由于全身毒性、药物生物利用度差和多药耐药（MDR），其治疗存在局限性。纳米技术的最新进展已经彻底改变了药物输送系统（DDS），提供了有针对性的、可控的和协同的治疗策略。这篇综述探讨了尖端的微纳米载体，包括脂质体（Lips），纳米颗粒（NPs），金属有机框架（MOFs），外泌体和纳米纤维（NFs），旨在优化化疗药物的递送。关键设计参数严格分析其在优化药物包封，肿瘤特异性和生物相容性方面的作用。我们强调在载体工程创新，使生物调制。此外，联合化疗药物与siRNA、p -糖蛋白抑制剂或光热药物的共递送系统在体外和体内逆转耐多药方面都取得了显著的成功。尽管取得了进展，但诸如肿瘤异质性、长期载体安全性和成本效益等挑战仍需进一步研究。这篇综述强调了纳米技术驱动的DDS在精确肿瘤学中的变革潜力，为下一代治疗乳腺癌铺平了道路。

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引用次数: 0

A novel ROS switcher potentiates the type-I photodynamic effect of sodium zinc chlorophyllin against Pseudomonas aeruginosa in diabetic wounds 一种新的活性氧开关增强了叶绿素锌钠对糖尿病伤口铜绿假单胞菌的i型光动力作用

IF 6 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications

Pub Date : 2026-01-19 DOI: 10.1016/j.bioadv.2026.214721

Guodong Wang , Hongjie Chen , Lusheng Wu , Cai Yuan , Mingdong Huang

Skin wound infections present a serious threat to human health, with bacterial infections in diabetic wounds being particularly challenging due to impaired healing. Gram-negative bacteria are common pathogens in diabetic wound infections and often exhibit high resistance to conventional treatments, necessitating prolonged and costly therapies. Photodynamic antibacterial therapy (PACT) is regarded as a non-invasive and effective approach, with minimal risk of inducing resistance. Sodium zinc chlorophyllin (ZnChl), a water-soluble metalloporphyrin photosensitizer, offers multiple advantages such as low cost, high safety, and the ability to promote wound healing. However, its bactericidal efficacy against Gram-negative bacteria is limited under the hypoxic conditions typically found in diabetic wounds. Herein, we report a strategy to develop an efficient chlorophyll-based photosensitizer operating via an oxygen-independent Type I mechanism by combining ZnChl with inorganic salts (NaI, KBr, or KI). This approach significantly improved antibacterial performance, particularly with NaI or KI. Upon 630 nm light irradiation, low concentrations of KI (5 mM) markedly enhanced the bactericidal effect of ZnChl (50 μM), reducing the survival of three Gram-negative bacterial strains by 3 logs (99.9%). Interestingly, we further discovered that KI acts as a “reactive oxygen species (ROS) converter,” shifting the photodynamic mechanism of ZnChl from a mixed Type I/Type II mode to a dominant Type I mechanism, generating highly reactive hydroxyl radicals (OH). Moreover, the ZnChl-KI combination demonstrated significant therapeutic efficacy in treating Pseudomonas aeruginosa-infected diabetic wounds without inducing apparent toxicity in rats. This work provides a foundation for developing efficient and economical Type I photosensitizers for the treatment of bacterial infections in diabetic wounds.

皮肤伤口感染对人类健康构成严重威胁，由于愈合受损，糖尿病伤口的细菌感染尤其具有挑战性。革兰氏阴性菌是糖尿病伤口感染的常见病原体，通常对常规治疗表现出高度耐药性，需要长期和昂贵的治疗。光动力抗菌治疗（PACT）被认为是一种无创和有效的方法，具有最小的风险诱导耐药。叶绿素锌钠（ZnChl）是一种水溶性金属卟啉光敏剂，具有成本低、安全性高、促进伤口愈合等优点。然而，它对革兰氏阴性菌的杀菌效果在糖尿病伤口缺氧条件下是有限的。在此，我们报告了一种策略，通过将ZnChl与无机盐（NaI， KBr或KI）结合，开发出一种高效的叶绿素光敏剂，通过不依赖氧的I型机制工作。这种方法显著提高了抗菌性能，特别是与NaI或KI。在630 nm光照射下，低浓度KI （5 mM）显著增强ZnChl （50 μM）的杀菌效果，使3株革兰氏阴性菌的存活率降低3倍（99.9%）。有趣的是，我们进一步发现KI作为“活性氧（ROS）转换器”，将ZnChl的光动力学机制从混合的I型/ II型模式转变为显性的I型机制，产生高活性的羟基自由基（OH）。此外，ZnChl-KI联合治疗铜绿假单胞菌感染的糖尿病大鼠伤口具有显著的治疗效果，且无明显毒性。本研究为开发高效经济的I型光敏剂治疗糖尿病创面细菌感染奠定了基础。

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引用次数: 0

A novel polymeric hydrogel of Periplaneta americana polysaccharide loaded with kaempferol-chitosan microspheres, which possesses antibacterial, hemostatic, antioxidant and wound healing promoting activities 一种负载山奈酚壳聚糖微球的新型美洲大蠊多糖聚合水凝胶，具有抗菌、止血、抗氧化和促进伤口愈合的活性

IF 6 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications

Pub Date : 2026-01-19 DOI: 10.1016/j.bioadv.2026.214722

Yiting Yang , Hanwen Hu , Xinxin Zhang , Seiichi Tokula , Manting Zhao , Ling He , Hujing Zhang , Qiao Liu , Qiang Fu , Qin Song

Periplaneta americana, as a traditional Chinese medicine, is widely used in wound repair. We isolated and purified a heteropolysaccharide PAP55–1-2 (8.13 kDa) from Periplaneta americana medicinal materials, which is mainly composed of N-acetylgalactosamine, galactose, glucose and mannose, etc. Among them, the content of N-acetylgalactosamine is relatively high, with a large number of N-acetyl groups. Through the study of structure-activity relationship, it was found that the N-acetyl groups play an important role in the process of wound healing. Therefore, PAP55–1-2 was reacted with N, N′‑carbonyldiimidazole (CDI) in DMSO at 50 °C for 3 h, and then ethylenediamine was added and reacted for 48 h. By using this mild method, the amino-modified Periplaneta americana polysaccharide (PAP-AM) was prepared while retaining the N-acetyl groups and their activity, and then reacted with aldehyde-modified chondroitin sulfate (Chs-CHO) to prepare a hydrogel. The cross-linking mechanism is attributed to the Schiff base reaction between amino and aldehyde groups. Subsequently, we prepared kaempferol (Kae)-chitosan (CS) microspheres with a diameter of 89.01 μm by a modified emulsification method, and then embedded them into high-dose PAP-AM hydrogel (PH-C) to produce a composite scaffold. The composite hydrogel Kae-CS@PH-C has good biodegradability, injectability, adhesion, self-healing and mechanical properties. In addition, it can accelerate the wound healing process in mice through antibacterial, hemostatic, antioxidant and immunomodulatory activities. These experiments indicate that the composite hydrogel has potential for biological applications.

美洲大蠊是一种中药，广泛用于伤口修复。从美洲大蠊药材中分离纯化了一种杂多糖PAP55-1-2 (8.13 kDa)，主要由n -乙酰半乳糖、半乳糖、葡萄糖和甘露糖等组成。其中，n -乙酰半乳糖胺含量较高，具有大量的n -乙酰基。通过构效关系的研究发现，n -乙酰基在伤口愈合过程中起着重要的作用。因此，将PAP55-1-2与N， N′-羰基二咪唑（CDI）在DMSO中50℃反应3 h，然后加入乙二胺反应48 h。采用这种温和的方法，在保留N-乙酰基及其活性的情况下制备氨基修饰的美洲大蠊多糖（PAP-AM），然后与醛修饰的硫酸软骨素（Chs-CHO）反应制备水凝胶。交联机理归因于氨基和醛基之间的席夫碱反应。随后，我们采用改性乳化法制备了直径为89.01 μm的山奈酚(Kae)-壳聚糖（CS）微球，并将其包埋在大剂量PAP-AM水凝胶（PH-C）中制备复合支架。复合水凝胶Kae-CS@PH-C具有良好的生物降解性、注射性、粘附性、自愈性和力学性能。此外，它还能通过抗菌、止血、抗氧化和免疫调节等作用加速小鼠伤口愈合过程。这些实验表明该复合水凝胶具有潜在的生物学应用前景。

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