Materials Science & Engineering C-Materials for Biological Applications最新文献_第6页

Cerium-based nanoplatforms inhibiting goblet cell–associated antigen passages to stabilize barrier function in ulcerative colitis 基于铈的纳米平台抑制杯状细胞相关抗原通道以稳定溃疡性结肠炎的屏障功能

IF 6 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications

Pub Date : 2026-01-06 DOI: 10.1016/j.bioadv.2026.214706

Mao Tang , Laixian Zhou , Haitao Ran

Ulcerative colitis (UC) is a chronic inflammatory disease of the colon characterized by recurrent mucosal inflammation and ulceration. Since managing UC remains challenging due to frequent therapeutic resistance and relapse, there is a pressing need for new strategies that target the underlying disease mechanisms to achieve long-term remission. Recent studies have highlighted the functional heterogeneity of intestinal goblet cells (GCs) beyond their classical role in mucus secretion. The goblet cell–associated passages (GAPs) have been identified as a luminal antigen delivery to lamina propria immune cells. Excessive GAP opening contributes to barrier dysfunction and mucosal inflammation, suggesting that GAPs are a promising therapeutic target. In this study, we designed a chondroitin sulfate–coated cerium nanoplatform (CS/CeO₂) loaded with the BAPTA-AM (BA) to stabilize the barrier function in colon. Briefly, BA was used to suppress excessive GAPs opening during the early stage of colitis, thereby alleviating abnormal immune activation. At the same time, CeO₂ served as a carrier to restrict the free diffusion of BA. Meanwhile, CS enabled efficient targeting of ulcerative lesions, allowing CeO₂ to fully exert its reactive oxygen species (ROS)–scavenging and CT imaging capabilities. Upon oral administration, BA-CS/CeO₂ successfully inhibited aberrant GAPs opening in DSS-induced colitis mice and enhanced intestinal barrier integrity, with restoration of mucus layer thickness and epithelial tight junctions, alongside reduced immune cell infiltration. Overall, this work leverages GC heterogeneity to re-establish intestinal barrier homeostasis, offering a promising nanotherapeutic strategy for UC through GAPs regulation, ROS scavenging, and CT monitoring.

溃疡性结肠炎（UC）是一种慢性结肠炎症性疾病，其特征是反复发生粘膜炎症和溃疡。由于UC的治疗经常出现耐药性和复发，因此管理UC仍然具有挑战性，迫切需要针对潜在疾病机制的新策略来实现长期缓解。最近的研究强调了肠杯状细胞（GCs）的功能异质性，超出了它们在粘液分泌中的经典作用。杯状细胞相关传代（GAPs）已被确定为固有层免疫细胞的腔内抗原递送。GAP开放过度会导致屏障功能障碍和粘膜炎症，这表明GAP是一个很有前景的治疗靶点。在这项研究中，我们设计了一种装载BAPTA-AM （BA）的硫酸软骨素包被的铈纳米平台（CS/ ceo2）来稳定结肠中的屏障功能。简单地说，BA可以抑制结肠炎早期gap的过度开放，从而减轻异常的免疫激活。同时，CeO₂起到了限制BA自由扩散的载体作用。同时，CS能够有效靶向溃疡病变，使CeO₂充分发挥其活性氧（ROS）清除能力和CT成像能力。经口服给药，BA-CS/CeO 2成功抑制dss诱导的结肠炎小鼠的异常间隙打开，增强肠屏障的完整性，恢复黏液层厚度和上皮紧密连接，同时减少免疫细胞浸润。总的来说，这项工作利用GC异质性来重建肠屏障稳态，通过gap调节、ROS清除和CT监测为UC提供了一种有前途的纳米治疗策略。

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引用次数: 0

Prevention of brain scarring during cranial reconstruction through a bioactive polymer coating 利用生物活性聚合物涂层预防颅骨重建过程中的脑瘢痕

IF 6 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications

Pub Date : 2026-01-05 DOI: 10.1016/j.bioadv.2025.214696

Sara Shakibania , Daria Niewolik , Taral Patel , Małgorzata Bogusz , Roman Turczyn , Marcin Basiaga , Katarzyna Jaszcz , Barbara Bednarczyk-Cwynar , Magdalena Skonieczna , Katarzyna Krukiewicz

Although cranioplasty is a long-established surgical procedure used to repair or reshape skull defects, it is not devoid of side effects arising from the formation of a scar tissue between dura, implant, and subcutaneous tissue. To minimize these complications, we developed a bioactive coating comprising poly(vinyl alcohol) (PVA) electrospun fibers loaded with a polyanhydride based on betulin disuccinate and a tricarboxylic derivative of poly(ethylene glycol) (PEG-DBB), suitable for the modification of the surface of titanium alloys. Although cell viability assays proved biocompatibility of fibers, PVA/PEG-DBB was found to decrease the adhesion of neuroblastoma cells by 84 % and the adhesion of fibroblast cells by 11 %, while increasing the confluency of osteosarcoma cells by 40 %. To assess the potential of the PVA/PEG-DBB fibrous coating, it was deposited onto the surface of Ti-6Al-4V alloy discs via electrospinning. In vitro bioactivity of samples was assessed via immersion in a simulated body fluid for 21 days, revealing facilitated formation of hydroxyapatite layer (Ca/P ratio of 1.74) in the presence of PVA/PEG-DBB (54.0 ± 6.5 % surface coverage, 11.7 ± 1.3 μm thickness), as confirmed by scanning electron microscopy and Raman spectroscopy. Adhesion strength of the coating to the substrate (between 1.7 and 2.7 N) was quantified using scratch testing under progressive loading, with critical load values determined to evaluate interfacial bonding integrity. By integrating biocompatibility, modulated adhesiveness, and mechanical resilience, PVA/PEG-DBB coating presents a promising approach for enhancing cranioplasty outcomes and minimizing adhesion-related complications.

尽管颅骨成形术是一种长期建立的用于修复或重塑颅骨缺损的外科手术，但它并非没有由硬脑膜、植入物和皮下组织之间形成疤痕组织引起的副作用。为了尽量减少这些并发症，我们开发了一种生物活性涂层，该涂层由聚乙烯醇（PVA）静电纺丝纤维组成，负载基于白桦二酸酯的聚酸酐和聚乙二醇的三羧基衍生物（PEG-DBB），适用于钛合金表面改性。虽然细胞活力测定证实了纤维的生物相容性，但发现PVA/PEG-DBB可使神经母细胞瘤细胞的粘附性降低84%，成纤维细胞的粘附性降低11%，同时使骨肉瘤细胞的融合性提高40%。为了评估PVA/PEG-DBB纤维涂层的潜力，通过静电纺丝将其沉积在Ti-6Al-4V合金表面。通过模拟体液浸泡21天评估样品的体外生物活性，发现PVA/PEG-DBB（54.0±6.5%表面覆盖率，11.7±1.3 μm厚度）存在时，羟基磷灰石层的形成（Ca/P比值为1.74），扫描电镜和拉曼光谱证实了这一点。通过渐进式载荷下的划痕测试来量化涂层与基体的粘附强度（在1.7到2.7 N之间），并确定临界载荷值来评估界面粘合完整性。PVA/PEG-DBB涂层通过整合生物相容性、可调节的粘连性和机械弹性，为提高颅骨成形术效果和减少粘连相关并发症提供了一种有前途的方法。

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引用次数: 0

Amine functionalized TiO2 doped mesoporous bioactive glass coated curcumin infused 3d printed poly (L-lactic acid): A promising multifunctional scaffold with enhanced osteogenesis and tumour relapse inhibition characteristics 胺功能化TiO2掺杂介孔生物活性玻璃涂层姜黄素注入3d打印聚l -乳酸：一种具有增强成骨和抑制肿瘤复发特性的多功能支架

IF 6 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications

Pub Date : 2026-01-05 DOI: 10.1016/j.bioadv.2026.214705

Shubham Pant , Sravanthi Loganathan , Ravi Babu Valapa

The two main clinical problems with post-operative metastatic bone disease are high death rate and a notable local recurrence rate. When diseased bone sections are removed from patients, they suffer from significant bone defects that cannot be repaired on their own, and therefore require a support mechanism via the incorporation of scaffolds. In such cases, multifunctional scaffolds are deemed to be an appropriate option with promising potential as bone substitutes and limit the recurrence of post-operative tumour cells. This can be achieved via constructing a multi-functional scaffold incorporated with therapeutic compounds that helps in the prevention of tumour recurrence and improved surface characteristics for achieving enhanced osteogenesis. In the current work, a multi-functional scaffold is constructed via coating of amine functionalized Titanium dioxide (TiO₂) doped mesoporous bioactive glass (MBG) on 3D printed curcumin infused PLA support. The influence of curcumin incorporation towards inhibiting the growth of osteosarcoma cells and surface coating for enhanced osteogenesis of adipose-derived mesenchymal stem cells (AD-MSCs) is investigated. In this study, MBG is doped with TiO₂ and surface grafted with amine groups to facilitate coating on 3D printed PLA and curcumin infused PLA surface via EDC-NHS carbodiimide chemistry. As revealed by FESEM analysis, the bioactive coating was observed to induce in-vitro bioactivity on day 1 of SBF treatment. Most importantly, the release of curcumin shows antibacterial activity against gram-positive (∼90 %) and gram-negative bacterial strain (∼85 %) as well as cytotoxicity towards osteosarcoma cells lines (∼ 90 % cell apoptosis observed after 14 days of culture). The TiO₂ doped MBG promotes osteogenic differentiation, observed with a significant increase in calcium mineralization (p < 0.0001) and Alkaline phosphatase activity (p < 0.0001) compared to PLA scaffolds via mild release of reactive oxygen species, which facilitates production of antioxidant proteins and ultimately promotes upregulation of early and late osteogenic markers.

术后转移性骨病的两个主要临床问题是高死亡率和显著的局部复发率。当患病的骨切片从患者身上移除时，患者会遭受严重的骨缺损，这些骨缺损无法自行修复，因此需要通过支架的结合来支持机制。在这种情况下，多功能支架被认为是一种合适的选择，作为骨替代品有很大的潜力，并且可以限制术后肿瘤细胞的复发。这可以通过构建与治疗化合物结合的多功能支架来实现，该支架有助于预防肿瘤复发和改善表面特性，从而实现增强的成骨。在目前的工作中，通过在3D打印的姜黄素注入PLA支架上涂覆胺功能化二氧化钛（TiO2）掺杂的介孔生物活性玻璃（MBG），构建了多功能支架。研究了姜黄素掺入对抑制骨肉瘤细胞生长和表面涂层促进脂肪源性间充质干细胞（AD-MSCs）成骨的影响。在本研究中，通过EDC-NHS碳二亚胺化学，在MBG中掺杂TiO2并在其表面接枝胺基，以促进3D打印PLA和姜黄素注入PLA表面的涂层。FESEM分析显示，生物活性包衣在SBF处理第1天诱导体外生物活性。最重要的是，姜黄素的释放显示出对革兰氏阳性（~ 90%）和革兰氏阴性菌株（~ 85%）的抗菌活性，以及对骨肉瘤细胞系的细胞毒性（培养14天后观察到~ 90%的细胞凋亡）。TiO2掺杂的MBG促进成骨分化，与PLA支架相比，通过轻度释放活性氧，钙矿化（p < 0.0001）和碱性磷酸酶活性（p < 0.0001）显著增加，促进抗氧化蛋白的产生，最终促进早期和晚期成骨标志物的上调。

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引用次数: 0

An injectable dual-drug-loaded hydrogel for the treatment of bacterial endophthalmitis 一种用于治疗细菌性眼内炎的可注射双重药物负载水凝胶

IF 6 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications

Pub Date : 2026-01-04 DOI: 10.1016/j.bioadv.2026.214704

Jianzhong He , Huixiang Ma , Yan Zhang , Daokai Huang , Zhou Zhou , Meng Cheng , Lei Wang , Yinzi Piao , Xiaojuan Hao

Bacterial endophthalmitis is a severe ocular infection that frequently leads to severe visual impairment or blindness. Current clinical treatments primarily rely on intravitreal antibiotics to eliminate pathogens; however, these often fail to control the excessive inflammatory response that contributes to irreversible retinal tissue damage. We developed an injectable thermosensitive hydrogel that treats bacterial endophthalmitis via a combined antibacterial and anti-inflammatory strategy. The hydrogel is constructed with a chitosan/β-glycerophosphate/gelatin platform co-loaded with moxifloxacin and a curcumin–cyclodextrin inclusion complex. In vitro injectability, rheological, and drug release studies demonstrated that the hydrogel precursor rapidly formed a gel following intravitreal injection and enabled sustained drug release. Rheological and swelling-degradation investigations confirmed its low mechanical modulus, minimal swelling capacity, and optimal biodegradation profile, thereby avoiding potential ocular discomfort during intraocular application. This system enables rapid release of moxifloxacin to eliminate bacteria, followed by sustained release of curcumin to mitigate inflammation and reduce inflammatory-mediated tissue injury. Both in vitro and in vivo evaluations demonstrated that the hydrogel showed a strong antibacterial efficacy against S. aureus and significant anti-inflammatory activity, leading to preserved retinal structure and improved visual function. The hydrogel exhibited excellent injectability, biodegradability, and biocompatibility. These properties support its potential for clinical application by providing sustained release of antibacterial and anti-inflammatory drugs for 14 days. To our knowledge, this also represents a novel application of such a dual-drug delivery strategy, specifically for treating S. aureus endophthalmitis. This approach underscores significant promise for future clinical investigation.

细菌性眼内炎是一种严重的眼部感染，经常导致严重的视力损害或失明。目前的临床治疗主要依靠玻璃体内抗生素来消除病原体；然而，这些往往不能控制过度的炎症反应，导致不可逆的视网膜组织损伤。我们开发了一种可注射的热敏水凝胶，通过抗菌和抗炎的联合策略治疗细菌性眼内炎。该水凝胶由壳聚糖/β-甘油磷酸/明胶平台与莫西沙星和姜黄素-环糊精包合物共同负载而成。体外注射性、流变学和药物释放研究表明，水凝胶前体在玻璃体内注射后迅速形成凝胶，并使药物持续释放。流变学和膨胀降解研究证实了其低机械模量，最小的膨胀能力和最佳的生物降解特征，从而避免了眼内应用时潜在的眼部不适。该系统能够快速释放莫西沙星以消除细菌，随后持续释放姜黄素以减轻炎症并减少炎症介导的组织损伤。体外和体内实验均表明，该水凝胶对金黄色葡萄球菌具有较强的抗菌作用和显著的抗炎活性，可保护视网膜结构，改善视觉功能。该水凝胶具有良好的注射性、生物可降解性和生物相容性。这些特性支持其临床应用的潜力，提供持续释放抗菌和抗炎药物14天。据我们所知，这也代表了这种双药递送策略的新应用，特别是治疗金黄色葡萄球菌眼内炎。这种方法强调了未来临床研究的重大前景。

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引用次数: 0

A dual-active biological scaffold with in situ loaded Abaloparatide and bone marrow-derived mesenchymal stem cells synergistically regulatory T cell to promote bone regeneration 一种含有原位负载Abaloparatide和骨髓间充质干细胞的双活性生物支架，协同调节T细胞促进骨再生

IF 6 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications

Pub Date : 2026-01-02 DOI: 10.1016/j.bioadv.2025.214700

Mingyu Jia , Zhihong Chen , Huajian Zhou , Haoran Jiang , Yukang Zhang , Yangyang Liu , Min Wu

Bone defect repair remains a significant challenge faced in clinical practice. Bone tissue engineering has emerged as an effective approach for bone defect repair due to its ample donor source and remarkable repair efficacy. In this study, we combined Abaloparatide (ABL) with bone marrow-derived mesenchymal stem cells (BMSCs) and utilized methacrylated gelatin (GelMA) as a matrix to successfully develop a bio-scaffold with dual activity (ABL@GelMA/BMSCs). BMSCs regulate the bone microenvironment by secreting cytokines, which promote bone repair through a paracrine mechanism. The addition of ABL not only facilitated the osteogenic differentiation and paracrine function of BMSCs but also significantly enhanced the bone induction capability of the scaffold, as evidenced by the marked upregulation of osteogenic markers (ALP, RUNX2, OCN). Furthermore, this bio-scaffold was able to upregulate the Foxp3 gene, inducing Foxp3+ regulatory T cells, thereby modulating the local immune microenvironment to promote bone formation. After implantation of this bio-scaffold into a critical-sized cranial defect model, histological results demonstrated a higher bone mineral density (BMD), and CT reconstruction indicated good healing of the defect. In summary, this bio-scaffold exhibits a powerful capacity for bone defect repair while improving the bone microenvironment through the synergistic modulation of regulatory T cells, providing a new strategy for bone defect repair.

骨缺损修复是临床实践中面临的重大挑战。骨组织工程因其供体来源丰富、修复效果显著而成为骨缺损修复的有效途径。在这项研究中，我们将Abaloparatide （ABL）与骨髓间充质干细胞（BMSCs）结合，并利用甲基丙烯酸明胶（GelMA）作为基质，成功地开发了具有双重活性的生物支架（ABL@GelMA/BMSCs）。骨髓间充质干细胞通过分泌细胞因子调节骨微环境，细胞因子通过旁分泌机制促进骨修复。ABL的加入不仅促进了骨髓间充质干细胞的成骨分化和旁分泌功能，还显著增强了支架的成骨诱导能力，成骨标志物（ALP、RUNX2、OCN）明显上调。此外，该生物支架能够上调Foxp3基因，诱导Foxp3+调节性T细胞，从而调节局部免疫微环境，促进骨形成。将该生物支架植入临界尺寸颅骨缺损模型后，组织学结果显示骨矿物质密度（BMD）较高，CT重建显示缺损愈合良好。综上所述，该生物支架具有强大的骨缺损修复能力，同时通过调节性T细胞的协同调节改善骨微环境，为骨缺损修复提供了一种新的策略。

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引用次数: 0

Paotianxiong polysaccharides as a stabilizer for protein nanoparticles and construction of an enhanced gastric acid-responsive hydrogel for delivery of fisetin to alleviate alcoholic gastric injury 泡天熊多糖作为蛋白质纳米颗粒的稳定剂和增强胃酸反应水凝胶的构建，用于递送非西汀以减轻酒精性胃损伤

IF 6 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications

Pub Date : 2026-01-02 DOI: 10.1016/j.bioadv.2025.214702

Qiao Ren , Jiaxin Jiang , Chenghao He , Jiayizhuo Liu , Jingcheng Jiang , Shuguang Hou , Qinwan Huang

Long-term excessive alcohol intake can easily lead to gastritis, gastric ulcer, and gastric bleeding. To address fisetin's (FIT) limitations in ameliorating alcohol-induced gastric injury while resolving stability issues of pH-driven albumin nanoparticles and oral protein-based drug delivery carriers, this study innovatively employed Paotianxiong polysaccharides (PP) as a stabilizer for protein nanoparticles and constructed an enhanced gastric acid-responsive hydrogel for delivery of FIT to alleviate alcohol-induced gastric injury. Through a green strategy (pH-driven method), albumin-PP composite nanoparticles were fabricated to improve FIT's solubility, stability, and bioavailability. The nanoparticles exhibit a uniform particle size and excellent dispersibility. In different pH acidic environments and ionic concentrations, the addition of PP significantly boosts the nanoparticles' stability. To further enhance gastric retention and sustained drug-release properties, an acid-responsive hydrogel was designed using SA and Ca²⁺, and carboxymethyl chitosan and PP were introduced to optimize the hydrogel's toughness, bioadhesion, and biocompatibility. This gastric-retentive delivery system formed by incorporating drug-loaded composite nanoparticles into the hydrogel shows a denser network structure, excellent mechanical properties, bioadhesion, sustained-release and gastric-retentive properties. In vivo studies demonstrate that the drugs delivered by this system exhibit superior distribution characteristics in the stomach and liver. They can effectively alleviate alcohol-induced gastric injury by accelerating hepatic ethanol metabolism, regulating oxidative stress and inflammatory responses in gastric tissues, and inhibiting pyroptosis. Finally, this delivery system is composed of biomaterials, conferring excellent safety. This delivery system provides new strategies for improving the stability of protein nanoparticles and ameliorating alcohol-induced gastric injury, respectively.

长期过量饮酒容易导致胃炎、胃溃疡和胃出血。为了解决非西汀（FIT）在改善酒精性胃损伤方面的局限性，同时解决ph驱动的白蛋白纳米颗粒和口服蛋白质为基础的药物递送载体的稳定性问题，本研究创新性地采用保天熊多糖（PP）作为蛋白质纳米颗粒的稳定剂，构建了一种增强的胃酸反应水凝胶，用于递送FIT以减轻酒精性胃损伤。通过绿色策略（ph驱动法），制备了白蛋白- pp复合纳米颗粒，以提高FIT的溶解度、稳定性和生物利用度。纳米颗粒具有均匀的粒径和良好的分散性。在不同pH酸性环境和离子浓度下，PP的加入显著提高了纳米颗粒的稳定性。为了进一步提高胃潴留和药物缓释性能，以SA和Ca2+为原料设计了酸反应型水凝胶，并添加羧甲基壳聚糖和PP优化了水凝胶的韧性、生物粘附性和生物相容性。这种将载药复合纳米颗粒掺入水凝胶形成的保胃给药系统具有更致密的网络结构、优异的力学性能、生物粘附性、缓释性和保胃性能。体内研究表明，通过该系统输送的药物在胃和肝脏中表现出优越的分布特性。它们可以通过加速肝脏乙醇代谢，调节胃组织氧化应激和炎症反应，抑制焦亡，有效减轻酒精性胃损伤。最后，该输送系统由生物材料组成，具有优异的安全性。该递送系统分别为提高蛋白质纳米颗粒的稳定性和改善酒精诱导的胃损伤提供了新的策略。

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引用次数: 0

Functional replacement of fetal bovine serum by extracts from Galdieria sulphuraria in muscle cell culture 肌细胞培养中硫铁蛭提取物对胎牛血清的功能替代研究

IF 6 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications

Pub Date : 2026-01-02 DOI: 10.1016/j.bioadv.2025.214701

Svenja Hütker , Hanna Wellkamp , Verena Tellstroem , Imke Lang , Felicitas Berger

The development of serum-free culture systems is a major challenge in the large-scale production of cultured meat, where myoblasts must be expanded and differentiated without the use of fetal bovine serum (FBS). FBS remains the gold standard in myoblast culture, yet it poses ethical, economic, and environmental concerns. In this study, we tested the hypothesis, that protein-rich extracts derived from the red microalga Galdieria sulphuraria can functionally replace FBS during proliferation and differentiation of murine C2C12 skeletal muscle myoblasts. Native algal protein extracts were prepared, heat-treated, and fractionated by ammonium sulfate precipitation. The effects of these extracts on cell viability, metabolic activity, proliferation kinetics and terminal myogenic differentiation were systematically assessed under serum-reduced and serum-free conditions. Crude extracts exhibited cytotoxicity, whereas heat-treated and fractionated extracts enhanced cell viability and metabolic activity during short-term culture. During long-term cultivation, gradual replacement of FBS with heat-treated algal extracts supported sustained myoblast growth, albeit with reduced proliferation rates, and preserved the capacity for differentiation into multinucleated, myosin heavy chain-positive myotubes. Comparative proteomic analysis revealed distinct protein profiles between crude and heat-treated extracts, suggesting that thermal processing removes inhibitory components while enriching bioactive protein fractions. Collectively, these results demonstrate that G. sulphuraria-derived protein extracts can support long-term myoblast proliferation and differentiation following appropriate adaption, highlighting their potential as a sustainable and animal-free alternative to serum-derived supplements in muscle cell culture systems.

无血清培养系统的开发是大规模生产培养肉的主要挑战，其中成肌细胞必须在不使用胎牛血清（FBS）的情况下扩增和分化。FBS仍然是成肌细胞培养的黄金标准，但它引起了伦理、经济和环境方面的关注。在本研究中，我们验证了从红色微藻中提取的富含蛋白质的提取物在小鼠C2C12骨骼肌成肌细胞增殖和分化过程中可以功能性地替代FBS的假设。制备了天然海藻蛋白提取物，对其进行热处理，并用硫酸铵沉淀法对其进行分馏。在血清还原和无血清条件下，系统评估了这些提取物对细胞活力、代谢活性、增殖动力学和终肌分化的影响。粗提取物表现出细胞毒性，而热处理和分离提取物在短期培养中增强了细胞活力和代谢活性。在长期培养过程中，逐渐用经过热处理的藻类提取物替代FBS支持了成肌细胞的持续生长，尽管增殖率降低，并保留了向多核、肌球蛋白重链阳性肌管分化的能力。比较蛋白质组学分析揭示了粗提取物和热处理提取物之间不同的蛋白质谱，表明热处理去除了抑制成分，同时丰富了生物活性蛋白组分。总的来说，这些结果表明，在适当的适应后，G. suluraria来源的蛋白质提取物可以支持成肌细胞的长期增殖和分化，突出了它们作为肌肉细胞培养系统中血清来源补充剂的可持续和无动物替代品的潜力。

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引用次数: 0

Engineering of green sterilization technology to obtain biocompatible aerogels: Supercritical CO2 versus ethylene oxide and gamma radiation 获得生物相容性气凝胶的绿色灭菌技术工程：超临界CO2与环氧乙烷和伽马辐射

IF 6 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications

Pub Date : 2026-01-02 DOI: 10.1016/j.bioadv.2025.214698

María Carracedo-Pérez , Antonella Caterina Boccia , Inés Ardao , Cláudia P. Passos , Víctor Santos-Rosales , Beatriz Santos , Fabio Bernardo , María Blanco-Vales , Beatriz Magariños , Carlos A. García-González

The growing relevance of aerogels in biomedicine demands the choice of compatible sterilization techniques with these materials. Conventional methods, such as ethylene oxide (EO) and gamma radiation (γ-rays) sterilization, have significant drawbacks while facing important environmental restrictions. In this study, supercritical CO₂ (scCO₂) sterilization is tested for polysaccharide (starch and alginate) aerogels as an eco-friendly alternative to conventional procedures. Three post-processing treatments under different CO₂ exposure regimes (static, dynamic and combined) and in the presence of H₂O₂ as additive were developed and assessed to reach sterility assurance levels (SAL) below 10⁻⁶. After sterilization, a vacuum treatment was implemented to ensure a low residual presence of H₂O₂ in the aerogels so that the material biocompatibility was not compromised according to in vitro cell tests with fibroblasts. The residual adsorbed H₂O₂ was quantified for the first time in aerogels by nuclear magnetic resonance spectroscopy. The effects of the supercritical sterilization treatments on the textural and chemical properties of the aerogels were evaluated and compared to those treated with EO and γ-rays. Results highlight the unique efficiency of scCO₂ sterilization as a post-processing method that preserves the aerogel structure while offering an eco-sustainable potential for producing sterile and biocompatible materials.

随着气凝胶在生物医学中的应用越来越广泛，需要选择与这些材料相容的灭菌技术。传统的方法，如环氧乙烷（EO）和伽马射线（γ射线）灭菌，在面临重要的环境限制时具有明显的缺点。在这项研究中，超临界CO2 （scCO2）灭菌测试了多糖（淀粉和海藻酸盐）气凝胶作为传统程序的环保替代方案。在不同的CO2暴露机制下（静态、动态和联合），以及在H2O2作为添加剂存在的情况下，开发并评估了三种后处理方法，以达到低于10−6的无菌保证水平（SAL）。灭菌后，进行真空处理，以确保气凝胶中H2O2的残留量低，这样根据成纤维细胞的体外细胞试验，材料的生物相容性不会受到损害。利用核磁共振波谱法首次定量测定了气凝胶中吸附的残余H2O2。评价了超临界灭菌处理对气凝胶结构和化学性质的影响，并与EO和γ射线处理进行了比较。结果强调了scCO2灭菌作为后处理方法的独特效率，它保留了气凝胶结构，同时为生产无菌和生物相容性材料提供了生态可持续的潜力。

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引用次数: 0

Advances in functionalized biodegradable biliary stents: From material design to clinical translation 功能化可生物降解胆道支架的研究进展：从材料设计到临床应用

IF 6 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications

Pub Date : 2025-12-31 DOI: 10.1016/j.bioadv.2025.214699

Shuiguo Tang , Rongji Ye , Guanzhi Lai , Haoqing Xu , Yu Mao , Xiangling Wei , Chengjun Sun , Wuzheng Xia , Ming Han , Luybov Mikhailovna Akhmedova , Erkin Isakovich Musabaev , Sunrui Chen , Shaojun Shi , Linwei Wu

Biliary stricture, primarily caused by inflammation, hepatobiliary and pancreatic malignancies and other pathological factors, often leads to impaired bile drainage and subsequent poor clinical outcomes. While biliary stent implantation has become a standard palliative and therapeutic intervention, conventional plastic and metal stents are limited by issues such as biofilm formation, epithelial hyperplasia, and the necessity for secondary removal surgery. The emergence of biodegradable stents offers a promising alternative by providing temporary mechanical support and controlled in vivo degradation, thereby eliminating the need for retrieval procedures. However, single-material stents often fail to address the complex and varied clinical demands, particularly in cases involving biliary fibrosis, infection, or malignancy. This review comprehensively summarizes recent advances in functionalized biodegradable biliary stents, referring to additional capabilities beyond their primary mechanical function, with a focus on material innovations and their enhanced functionalities such as anti-fibrotic, anti-tumor, antimicrobial, anti-corrosion, and visualizable properties. We also discuss integrated therapeutic strategies, such as drug-eluting systems and tissue-engineered scaffolds, alongside manufacturing breakthroughs like 3D printing. Furthermore, we also highlight the persistent translational challenges and preclinical limitations of current biodegradable stent technologies, while outlining promising directions for future research to bridge the gap between experimental development and clinical application.

胆道狭窄主要由炎症、肝胆胰恶性肿瘤等病理因素引起，常导致胆汁引流受损，临床预后较差。虽然胆道支架植入已成为标准的姑息性和治疗性干预措施，但传统的塑料和金属支架受到生物膜形成、上皮增生和需要二次切除手术等问题的限制。生物可降解支架的出现提供了一种有希望的替代方案，它提供了暂时的机械支持和体内降解控制，从而消除了对回收程序的需要。然而，单材料支架往往不能满足复杂多样的临床需求，特别是在胆道纤维化、感染或恶性肿瘤的病例中。本文全面总结了功能化生物可降解胆道支架的最新进展，涉及其主要机械功能之外的附加功能，重点介绍了材料创新及其增强功能，如抗纤维化、抗肿瘤、抗菌、防腐和可视化性能。我们还讨论了综合治疗策略，如药物洗脱系统和组织工程支架，以及制造突破，如3D打印。此外，我们还强调了当前生物可降解支架技术持续存在的转化挑战和临床前局限性，同时概述了未来研究的有希望的方向，以弥合实验开发和临床应用之间的差距。

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Nanoplatform for renal ischemia-reperfusion injury repair: Modulating macrophage polarization, oxidative stress, and mitophagy 纳米平台用于肾缺血再灌注损伤修复：调节巨噬细胞极化、氧化应激和有丝分裂

IF 6 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications

Pub Date : 2025-12-30 DOI: 10.1016/j.bioadv.2025.214697

Mu He , Ting Liu , Hongchao Zhao , Tianran Zheng , Maonan Chen , Hengcheng Zhu , Kang Yang

Acute kidney injury (AKI) is a common clinical condition characterized by high morbidity and mortality rates, with a notable lack of effective therapeutic drugs. Complex pathological processes—such as oxidative stress overload, aberrant macrophage polarization, mitochondrial dysfunction, and renal tubular epithelial cell apoptosis—contribute to the current absence of effective clinical treatments for AKI. Although mesoporous cerium dioxide nanospheres have been widely applied in various diseases due to their remarkable ROS-scavenging and drug-loading capabilities, their poor targeting ability limits their use in ischemia-reperfusion injury models. To address this, we developed a multifunctional nanoplatform RGD-CeO₂@Que. based on mesoporous hollow cerium dioxide (AhCeO₂). This system achieves targeted accumulation in injured kidneys by binding to integrin αvβ3 receptors, which are overexpressed under oxidative stress. Through the Nrf2/HO-1/GPX4/SOD1 pathway, it alleviates oxidative stress and reduces apoptosis. Moreover, the platform is loaded with the bioactive molecule quercetin to promote mitophagy in renal tubular epithelial cells (HK−2). In vivo studies demonstrated that RGD-CeO₂@Que. improves renal function, ameliorates pathological damage, and reduces inflammatory infiltration in AKI mice. In summary, this integrated nanoplatform combines multiple restorative mechanisms, offering a novel and targeted therapeutic strategy for AKI induced by renal ischemia-reperfusion injury.

急性肾损伤（Acute kidney injury， AKI）是一种常见的临床疾病，其特点是发病率和死亡率高，缺乏有效的治疗药物。复杂的病理过程，如氧化应激超载、巨噬细胞异常极化、线粒体功能障碍和肾小管上皮细胞凋亡，导致目前缺乏有效的AKI临床治疗方法。虽然介孔二氧化铈纳米球因其出色的ros清除能力和载药能力已广泛应用于各种疾病，但其较差的靶向能力限制了其在缺血再灌注损伤模型中的应用。为了解决这个问题，我们开发了一种多功能纳米平台RGD-CeO₂@Que。基于介孔中空二氧化铈（ahceo2）。该系统通过与氧化应激下过度表达的整合素αvβ3受体结合，在受损肾脏中实现靶向蓄积。通过Nrf2/HO-1/GPX4/SOD1通路，缓解氧化应激，减少细胞凋亡。此外，该平台还装载了生物活性分子槲皮素，以促进肾小管上皮细胞（HK−2）的有丝分裂。体内研究表明，RGD-CeO 2 @Que。改善AKI小鼠肾功能，改善病理性损伤，减少炎症浸润。总之，这种集成的纳米平台结合了多种修复机制，为肾缺血再灌注损伤引起的AKI提供了一种新的靶向治疗策略。

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