Pub Date : 2025-03-24DOI: 10.1016/j.bioadv.2025.214290
Qing He , Chubo Huang , Yaming Lu , Yuan Zhao , Meiyi Xing , Xiangwen Wang , Binbin Sun , Yiqian Zhu
For clinical treatment of end-stage renal disease (ESRD) patients, the development of vascular grafts possessing both puncture resistance and anticoagulant properties remains crucial for arteriovenous fistula establishment. In this study, small-diameter vascular conduits were engineered through electrospinning of polyurethane (PU) microfibers, incorporating polyethylene coil reinforcement within the graft wall architecture to confer kink resistance. The microporous structure of the grafts demonstrated effective self-sealing capabilities following needle perforation. Additionally, heparin immobilization was implemented on the luminal surface to optimize thromboresistance. Large animal implantation studies revealed that the PU vascular grafts exhibited immediate puncture feasibility, superior puncture durability, and maintained excellent hemodynamic patency in vivo, demonstrating significant translational potential for clinical hemodialysis applications.
{"title":"Developing an anticoagulant microfibrous vascular graft with enhanced kink resistance and self-sealing capabilities","authors":"Qing He , Chubo Huang , Yaming Lu , Yuan Zhao , Meiyi Xing , Xiangwen Wang , Binbin Sun , Yiqian Zhu","doi":"10.1016/j.bioadv.2025.214290","DOIUrl":"10.1016/j.bioadv.2025.214290","url":null,"abstract":"<div><div>For clinical treatment of end-stage renal disease (ESRD) patients, the development of vascular grafts possessing both puncture resistance and anticoagulant properties remains crucial for arteriovenous fistula establishment. In this study, small-diameter vascular conduits were engineered through electrospinning of polyurethane (PU) microfibers, incorporating polyethylene coil reinforcement within the graft wall architecture to confer kink resistance. The microporous structure of the grafts demonstrated effective self-sealing capabilities following needle perforation. Additionally, heparin immobilization was implemented on the luminal surface to optimize thromboresistance. Large animal implantation studies revealed that the PU vascular grafts exhibited immediate puncture feasibility, superior puncture durability, and maintained excellent hemodynamic patency <em>in vivo</em>, demonstrating significant translational potential for clinical hemodialysis applications.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"173 ","pages":"Article 214290"},"PeriodicalIF":5.5,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143697056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-24DOI: 10.1016/j.bioadv.2025.214291
Baoyan Liang , Ruoxue Bai , Jiayang Wang , Shuyang Shi , Yajie Guo , Qi Wang , Han Peng , Jiezhang Tang , Shuai Liu , Jun Zhu , Chenggang Yi , Mengmeng Hou , Huichen Li
Background
Skin dilation generates “extra” skin tissue through mechanical traction, but its effectiveness is limited by the proliferation capacity of keratinocytes, fibroblasts and the level of angiogenesis. Cutaneous application of drug and subcutaneous injection are common interventions to promote skin dilation, but they have defects such as uneven drug distribution, high risk of infection and single targeting. Although Acellular adipose matrix (AAM) has the potential to promote cell proliferation and angiogenesis, its hydrogel/powder dosage forms still need frequent injection, which limits clinical application.
Results
In this study, Acellular adipose matrix derived film (AAF) was successfully developed, and a flexible film was formed by acellular - lyophilized - enzymolysis - self-assembly process. In vitro experiments confirmed that AAF significantly promoted the activity of Human Immortalized Epidermal Cells (HaCaTs), Normal Skin Fibroblasts (NFbs) and Human Umbilical Endothelial Cells (HUVECs); It was also found that AAF can induce adipose mesenchymal stem cells (ASCs) to differentiate into adipocytes and promote subcutaneous fat regeneration. In vivo, the rat model showed that AAF wrapping expander could effectively improve the skin expansion efficiency, promote the skin thickness increase in the expanded area, and the density of new blood vessels was significantly increased compared with the comparative group, and there was no complication such as infection or skin collapse. It was found for the first time that AAF successfully formed new adipose tissue in the subcutaneous area.
Conclusion
AAF innovatively integrates the bionic structure of extracellular matrix and slow-release function, and solves the uneven drug distribution and associated infection risk of traditional intervention methods by regulating the synergistic regeneration of epidermodermis and vascular units. Its mechanical adaptability (dry toughness/wet plasticity) and the ability of inducing adipose regeneration provide a new strategy of both structural strengthening and metabolic support for skin dilation, also laying a mechanism and empirical foundation for clinical transformation of tissue engineering materials.
{"title":"Innovative applications of acellular adipose matrix derived film in skin soft tissue expansion","authors":"Baoyan Liang , Ruoxue Bai , Jiayang Wang , Shuyang Shi , Yajie Guo , Qi Wang , Han Peng , Jiezhang Tang , Shuai Liu , Jun Zhu , Chenggang Yi , Mengmeng Hou , Huichen Li","doi":"10.1016/j.bioadv.2025.214291","DOIUrl":"10.1016/j.bioadv.2025.214291","url":null,"abstract":"<div><h3>Background</h3><div>Skin dilation generates “extra” skin tissue through mechanical traction, but its effectiveness is limited by the proliferation capacity of keratinocytes, fibroblasts and the level of angiogenesis. Cutaneous application of drug and subcutaneous injection are common interventions to promote skin dilation, but they have defects such as uneven drug distribution, high risk of infection and single targeting. Although Acellular adipose matrix (AAM) has the potential to promote cell proliferation and angiogenesis, its hydrogel/powder dosage forms still need frequent injection, which limits clinical application.</div></div><div><h3>Results</h3><div>In this study, Acellular adipose matrix derived film (AAF) was successfully developed, and a flexible film was formed by acellular - lyophilized - enzymolysis - self-assembly process. In vitro experiments confirmed that AAF significantly promoted the activity of Human Immortalized Epidermal Cells (HaCaTs), Normal Skin Fibroblasts (NFbs) and Human Umbilical Endothelial Cells (HUVECs); It was also found that AAF can induce adipose mesenchymal stem cells (ASCs) to differentiate into adipocytes and promote subcutaneous fat regeneration. In vivo, the rat model showed that AAF wrapping expander could effectively improve the skin expansion efficiency, promote the skin thickness increase in the expanded area, and the density of new blood vessels was significantly increased compared with the comparative group, and there was no complication such as infection or skin collapse. It was found for the first time that AAF successfully formed new adipose tissue in the subcutaneous area.</div></div><div><h3>Conclusion</h3><div>AAF innovatively integrates the bionic structure of extracellular matrix and slow-release function, and solves the uneven drug distribution and associated infection risk of traditional intervention methods by regulating the synergistic regeneration of epidermodermis and vascular units. Its mechanical adaptability (dry toughness/wet plasticity) and the ability of inducing adipose regeneration provide a new strategy of both structural strengthening and metabolic support for skin dilation, also laying a mechanism and empirical foundation for clinical transformation of tissue engineering materials.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"173 ","pages":"Article 214291"},"PeriodicalIF":5.5,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143705559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-24DOI: 10.1016/j.bioadv.2025.214286
Phanindra Babu Kasi , Aleksandra Serafin , Liam O'Brien , Nick Moghbel , Lev N. Novikov , Peyman Kelk , Maurice N. Collins
Addressing the challenge of bone tissue regeneration requires creating an optimal microenvironment that promotes both osteogenesis and angiogenesis. Electroconductive scaffolds have emerged as promising solutions for bone regeneration; however, existing conductive polymers often lack biofunctionality and biocompatibility. In this study, we synthesized poly(3,4-ethylenedioxythiophene) nanoparticles (PEDOT NPs) using chemical oxidation polymerization and incorporated them into gelatin/hyaluronic acid/hydroxyapatite (Gel:HA:HAp) scaffolds to develop Gel:HA:HAp:PEDOT-NP scaffolds. Morphological analysis by scanning electron microscopy (SEM) showed a honeycomb-like structure with pores of 228–250 μm in diameter. The addition of the synthesized PEDOT NPs increased the conductive capabilities of the scaffolds to 1 × 10−6 ± 1.3 × 10−7 S/cm. Biological assessment of PEDOT NP scaffolds using human foetal osteoblastic 1.19 cells (hFOB), and human bone marrow-derived mesenchymal stem cells (hBMSCs) revealed enhanced cell proliferation and viability compared to control scaffold without NPs, along with increased osteogenic differentiation, evidenced by higher levels of alkaline phosphatase activity, osteopontin (OPN), alkaline phosphatase (ALP), and osteocalcin (OCN) expression, as observed through immunofluorescence, and enhanced expression of osteogenic-related genes. The conductive scaffold shows interesting mineralization capacity, as shown by Alizarin red and Osteoimage staining. Furthermore, PEDOT-NP scaffolds promoted angiogenesis, as indicated by improved tube formation abilities of human umbilical vein endothelial cells (HUVECs), especially at the higher concentrations of NPs. Overall, our findings demonstrate that the integration of PEDOT NPs scaffold enhances their conductive properties and promotes cell proliferation, osteogenic differentiation, and angiogenesis. Gel:HA:HAp:PEDOT-NP scaffolds exhibit promising potential as efficient biomaterials for bone tissue regeneration, offering a potential engineered platform for clinical applications.
{"title":"Electroconductive gelatin/hyaluronic acid/hydroxyapatite scaffolds for enhanced cell proliferation and osteogenic differentiation in bone tissue engineering","authors":"Phanindra Babu Kasi , Aleksandra Serafin , Liam O'Brien , Nick Moghbel , Lev N. Novikov , Peyman Kelk , Maurice N. Collins","doi":"10.1016/j.bioadv.2025.214286","DOIUrl":"10.1016/j.bioadv.2025.214286","url":null,"abstract":"<div><div>Addressing the challenge of bone tissue regeneration requires creating an optimal microenvironment that promotes both osteogenesis and angiogenesis. Electroconductive scaffolds have emerged as promising solutions for bone regeneration; however, existing conductive polymers often lack biofunctionality and biocompatibility. In this study, we synthesized poly(3,4-ethylenedioxythiophene) nanoparticles (PEDOT NPs) using chemical oxidation polymerization and incorporated them into gelatin/hyaluronic acid/hydroxyapatite (Gel:HA:HAp) scaffolds to develop Gel:HA:HAp:PEDOT-NP scaffolds. Morphological analysis by scanning electron microscopy (SEM) showed a honeycomb-like structure with pores of 228–250 μm in diameter. The addition of the synthesized PEDOT NPs increased the conductive capabilities of the scaffolds to 1 × 10<sup>−6</sup> ± 1.3 × 10<sup>−7</sup> S/cm. Biological assessment of PEDOT NP scaffolds using human foetal osteoblastic 1.19 cells (hFOB), and human bone marrow-derived mesenchymal stem cells (hBMSCs) revealed enhanced cell proliferation and viability compared to control scaffold without NPs, along with increased osteogenic differentiation, evidenced by higher levels of alkaline phosphatase activity, osteopontin (OPN), alkaline phosphatase (ALP), and osteocalcin (OCN) expression, as observed through immunofluorescence, and enhanced expression of osteogenic-related genes. The conductive scaffold shows interesting mineralization capacity, as shown by Alizarin red and Osteoimage staining. Furthermore, PEDOT-NP scaffolds promoted angiogenesis, as indicated by improved tube formation abilities of human umbilical vein endothelial cells (HUVECs), especially at the higher concentrations of NPs. Overall, our findings demonstrate that the integration of PEDOT NPs scaffold enhances their conductive properties and promotes cell proliferation, osteogenic differentiation, and angiogenesis. Gel:HA:HAp:PEDOT-NP scaffolds exhibit promising potential as efficient biomaterials for bone tissue regeneration, offering a potential engineered platform for clinical applications.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"173 ","pages":"Article 214286"},"PeriodicalIF":5.5,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143704824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-17DOI: 10.1016/j.bioadv.2025.214288
Vignesh Subramaniam , Carolina Abrahan , Brett R. Higgins , Steven J. Chisolm , Baleigh Sweeney , Senthilkumar Duraivel , Leandro Balzano-Nogueira , Tia Monjure , Chih-Yi Wang , Glyn D. Palmer , Thomas E. Angelini
To reduce costs and delays related to developing new and effective drugs, there is a critical need for improved human liver tissue models. Here we describe an approach for 3D bioprinting functional human liver tissue models, in which we fabricate disc-shaped structures (discoids) 200 μm in thickness and 1–3 mm in diameter from mixtures of cells and collagen-1, embedded in a highly permeable support medium made from packed polyethylene glycol (PEG) microgels. We demonstrate that the method is precise, accurate, and scalable; up to 100 tissues/h can be manufactured with a variability and error in diameter of about 4 %. Histologic and immunohistochemical evaluation of printed discs reveal self-organization, cell cohesion, and key liver marker expression. Over the course of three weeks in culture, the tissues stably synthesize albumin and urea at high levels, outperforming spheroid tissue models. We find the tissues express >100 genes associated with molecular absorption, distribution, metabolism, and excretion (ADME) at levels within the range of human liver. The liver tissue models exhibit enzymatic formation of metabolites after exposure to multiple test compounds. Together, these results demonstrate the promise of 3D printed discoids for pharmacological and toxicological applications.
{"title":"A functional human liver tissue model: 3D bioprinted co-culture discoids","authors":"Vignesh Subramaniam , Carolina Abrahan , Brett R. Higgins , Steven J. Chisolm , Baleigh Sweeney , Senthilkumar Duraivel , Leandro Balzano-Nogueira , Tia Monjure , Chih-Yi Wang , Glyn D. Palmer , Thomas E. Angelini","doi":"10.1016/j.bioadv.2025.214288","DOIUrl":"10.1016/j.bioadv.2025.214288","url":null,"abstract":"<div><div>To reduce costs and delays related to developing new and effective drugs, there is a critical need for improved human liver tissue models. Here we describe an approach for 3D bioprinting functional human liver tissue models, in which we fabricate disc-shaped structures (discoids) 200 μm in thickness and 1–3 mm in diameter from mixtures of cells and collagen-1, embedded in a highly permeable support medium made from packed polyethylene glycol (PEG) microgels. We demonstrate that the method is precise, accurate, and scalable; up to 100 tissues/h can be manufactured with a variability and error in diameter of about 4 %. Histologic and immunohistochemical evaluation of printed discs reveal self-organization, cell cohesion, and key liver marker expression. Over the course of three weeks in culture, the tissues stably synthesize albumin and urea at high levels, outperforming spheroid tissue models. We find the tissues express >100 genes associated with molecular absorption, distribution, metabolism, and excretion (ADME) at levels within the range of human liver. The liver tissue models exhibit enzymatic formation of metabolites after exposure to multiple test compounds. Together, these results demonstrate the promise of 3D printed discoids for pharmacological and toxicological applications.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"173 ","pages":"Article 214288"},"PeriodicalIF":5.5,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143645037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-14DOI: 10.1016/j.bioadv.2025.214287
Asef Raj , Sabrina Sharmin , Safrin Jannat , Saika Ahmed , Abu Bin Ihsan
In biomedical applications, bioadhesives have become a game-changer, offering novel approaches to tissue engineering, surgical adhesion, and wound healing. This comprehensive review paper provides a thorough analysis of bioadhesives and their categorization according to application site and crosslinking process, bonding efficacy, and mechanical characteristics. The use of bioadhesives to stop bleeding and seal leaks is also covered in the review. The article delves into the various crosslinking techniques used in bioadhesives, including chemical, physical, and hybrid approaches. It emphasizes on how these mechanisms control the adhesive's elasticity, durability, and structural integrity. In addition, the review looks at the mechanical strength of bioadhesives, taking important characteristics like shear strength, toughness, elasticity, and tensile strength into account. It is highlighted how important bioadhesives are to the life sciences because they drive innovation and interdisciplinary cooperation, address present healthcare issues, and create new avenues for therapeutic development. The paper also explores some vital characteristics of bioadhesives that, when strategically combined with one another, improve their efficacy and usefulness in a variety of surgical and medical applications. The analysis concludes by examining nature-inspired adhesives, including those based on geckos, mussels, and tannic acid, and their unique bonding mechanisms and potential for use in advanced biomedical applications.
{"title":"Innovative approaches in bioadhesive design: A comprehensive review of crosslinking methods and mechanical performance","authors":"Asef Raj , Sabrina Sharmin , Safrin Jannat , Saika Ahmed , Abu Bin Ihsan","doi":"10.1016/j.bioadv.2025.214287","DOIUrl":"10.1016/j.bioadv.2025.214287","url":null,"abstract":"<div><div>In biomedical applications, bioadhesives have become a game-changer, offering novel approaches to tissue engineering, surgical adhesion, and wound healing. This comprehensive review paper provides a thorough analysis of bioadhesives and their categorization according to application site and crosslinking process, bonding efficacy, and mechanical characteristics. The use of bioadhesives to stop bleeding and seal leaks is also covered in the review. The article delves into the various crosslinking techniques used in bioadhesives, including chemical, physical, and hybrid approaches. It emphasizes on how these mechanisms control the adhesive's elasticity, durability, and structural integrity. In addition, the review looks at the mechanical strength of bioadhesives, taking important characteristics like shear strength, toughness, elasticity, and tensile strength into account. It is highlighted how important bioadhesives are to the life sciences because they drive innovation and interdisciplinary cooperation, address present healthcare issues, and create new avenues for therapeutic development. The paper also explores some vital characteristics of bioadhesives that, when strategically combined with one another, improve their efficacy and usefulness in a variety of surgical and medical applications. The analysis concludes by examining nature-inspired adhesives, including those based on geckos, mussels, and tannic acid, and their unique bonding mechanisms and potential for use in advanced biomedical applications.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"173 ","pages":"Article 214287"},"PeriodicalIF":5.5,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143645036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-11DOI: 10.1016/j.bioadv.2025.214285
Andrea M. Rich , Leopold Berger , Robin Deller , Niccoló De Berardinis , Gry Hulsart Billström , Benedikt Helgason , Stephen J. Ferguson , Cecilia Persson , Jörg F. Löffler
In this study, we developed and characterized a fully biodegradable composite bone cement reinforced with short, randomly oriented amorphous magnesium fibers. Fibers of composition Mg60Zn35Ca5 (in at.%) with 50 μm diameter and 2 mm length were produced by wire spinning and then mixed with a magnesium calcium phosphate cement using fiber volume fractions between 10 and 20 %. The interface strength between the fibers and cement was improved by treating the fibers with diammonium hydrogen phosphate. Compared to the reference cement without fibers, flexural strength was increased by 18 % for the composites with 13 and 18 vol% fibers, and the work of fracture was increased by over 1000× in all cases (p < 0.05, n = 6). Immersion in simulated body fluid for two and four weeks showed that the cement's struvite phase degrades first, and overall, the composite degrades slower. The degradation rate can be tailored to the application by changing the fiber percentage or the cement/fiber composition. Murine pre-osteoblastic cells (MC3T3) cultured in extracts of reference and composite cements had significantly higher cell viability, and composites with 13 vol% fibers also had a significantly higher number of cells compared to the control, indicating that the fibers can enhance and promote pre-osteoblastic cell growth. The results demonstrate that amorphous magnesium fibers enhance both the mechanical and biological properties of ceramic bone cement, expanding their prospects for clinical application.
{"title":"Bioresorbable, amorphous magnesium-fiber reinforced bone cement with enhanced mechanical and biological properties","authors":"Andrea M. Rich , Leopold Berger , Robin Deller , Niccoló De Berardinis , Gry Hulsart Billström , Benedikt Helgason , Stephen J. Ferguson , Cecilia Persson , Jörg F. Löffler","doi":"10.1016/j.bioadv.2025.214285","DOIUrl":"10.1016/j.bioadv.2025.214285","url":null,"abstract":"<div><div>In this study, we developed and characterized a fully biodegradable composite bone cement reinforced with short, randomly oriented amorphous magnesium fibers. Fibers of composition Mg<sub>60</sub>Zn<sub>35</sub>Ca<sub>5</sub> (in at.%) with 50 μm diameter and 2 mm length were produced by wire spinning and then mixed with a magnesium calcium phosphate cement using fiber volume fractions between 10 and 20 %. The interface strength between the fibers and cement was improved by treating the fibers with diammonium hydrogen phosphate. Compared to the reference cement without fibers, flexural strength was increased by 18 % for the composites with 13 and 18 vol% fibers, and the work of fracture was increased by over 1000× in all cases (<em>p</em> < 0.05, <em>n</em> = 6). Immersion in simulated body fluid for two and four weeks showed that the cement's struvite phase degrades first, and overall, the composite degrades slower. The degradation rate can be tailored to the application by changing the fiber percentage or the cement/fiber composition. Murine pre-osteoblastic cells (MC3T3) cultured in extracts of reference and composite cements had significantly higher cell viability, and composites with 13 vol% fibers also had a significantly higher number of cells compared to the control, indicating that the fibers can enhance and promote pre-osteoblastic cell growth. The results demonstrate that amorphous magnesium fibers enhance both the mechanical and biological properties of ceramic bone cement, expanding their prospects for clinical application.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"173 ","pages":"Article 214285"},"PeriodicalIF":5.5,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143674918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-10DOI: 10.1016/j.bioadv.2025.214281
O. Contreras-Almengor , J. Ordoño , M. Li , E. Matykina , M. Avella , M. Echeverry-Rendón , A. Diaz-Lantada , J.M. Molina-Aldareguia
Laser powder bed fusion (LPBF) has emerged as a promising additive manufacturing technique to produce complex and custom-shaped NiTi devices, but precise control and characterization of the post-printing processing parameters are still required to achieve optimal surface properties and allow expanding the use of 3D-printed NiTi in cardiovascular applications. This work studies the effect of different surface post-processing techniques, including chemical etching, electropolishing and combinations of the two, on the surface properties and biological response of NiTi parts manufactured by LPBF. The different surface treatments resulted in changes in the roughness, wettability and corrosion resistance, which were closely correlated with the nature, microstructure and thickness of the surface oxide layers that form in each case. Furthermore, the biocompatibility of the different NiTi surfaces to human endothelial and smooth muscle cells, the main components of cardiovascular tissue, were also assessed. Interestingly, while the different surfaces showed high biocompatibility in terms of viability and proliferation, cells showed distinct morphology and orientation, as well as inflammatory response (IL-6). Finally, differences were also observed in the hemocompatibility of the 3D-printed NiTi surfaces to human blood. Overall, this work provides new insights for the wide use of additive manufacturing to develop personalized NiTi implants for cardiovascular applications.
{"title":"Effect of surface modification methods on 3D-printed NiTi alloys for cardiovascular applications","authors":"O. Contreras-Almengor , J. Ordoño , M. Li , E. Matykina , M. Avella , M. Echeverry-Rendón , A. Diaz-Lantada , J.M. Molina-Aldareguia","doi":"10.1016/j.bioadv.2025.214281","DOIUrl":"10.1016/j.bioadv.2025.214281","url":null,"abstract":"<div><div>Laser powder bed fusion (LPBF) has emerged as a promising additive manufacturing technique to produce complex and custom-shaped NiTi devices, but precise control and characterization of the post-printing processing parameters are still required to achieve optimal surface properties and allow expanding the use of 3D-printed NiTi in cardiovascular applications. This work studies the effect of different surface post-processing techniques, including chemical etching, electropolishing and combinations of the two, on the surface properties and biological response of NiTi parts manufactured by LPBF. The different surface treatments resulted in changes in the roughness, wettability and corrosion resistance, which were closely correlated with the nature, microstructure and thickness of the surface oxide layers that form in each case. Furthermore, the biocompatibility of the different NiTi surfaces to human endothelial and smooth muscle cells, the main components of cardiovascular tissue, were also assessed. Interestingly, while the different surfaces showed high biocompatibility in terms of viability and proliferation, cells showed distinct morphology and orientation, as well as inflammatory response (IL-6). Finally, differences were also observed in the hemocompatibility of the 3D-printed NiTi surfaces to human blood. Overall, this work provides new insights for the wide use of additive manufacturing to develop personalized NiTi implants for cardiovascular applications.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"173 ","pages":"Article 214281"},"PeriodicalIF":5.5,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143631984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-10DOI: 10.1016/j.bioadv.2025.214284
Shubham A. Salunkhe , Kiran Bajaj , Anupama Mittal
The clinical translation of therapeutic peptides and miRNAs is hindered by challenges such as short half-life, rapid clearance, and high dosage requirements. To address these limitations, cationic polymeric nanoparticles have been explored, but their development is often limited by toxicity and low transfection efficiency. In this study, we present a novel biocompatible delivery system using a combination of cationic and cholesterol-containing polymers to overcome these issues. The system was formulated into nanocomplexes (NCs) for the delivery of C Peptide (CPep) and miRNA-29b (miR29b). The formulation process involved electrostatic complexation of CPep/miR29b with the polymeric carriers, avoiding harsh conditions or chemical modifications. Native-PAGE, gel retardation, and heparin competition assays confirmed stable complexation. Cell uptake and transfection studies showed efficient delivery of both CPep and miR29b via NCs. In vitro models of oxidative and metabolic stress demonstrated enhanced cell viability with CPep NCs compared to free CPep, with increased glutathione and reduced nitric oxide levels. Similarly, miR29b NCs exhibited potent anti-inflammatory effects compared to free miR29b. This study presents a promising polymer-based carrier system for effective peptide and miRNA delivery through electrostatic interactions alone without any chemical reaction involved to preserve the integrity of the therapeutic.
{"title":"Cationic lipid-polymer hybrid carrier for delivery of miRNA and peptides","authors":"Shubham A. Salunkhe , Kiran Bajaj , Anupama Mittal","doi":"10.1016/j.bioadv.2025.214284","DOIUrl":"10.1016/j.bioadv.2025.214284","url":null,"abstract":"<div><div>The clinical translation of therapeutic peptides and miRNAs is hindered by challenges such as short half-life, rapid clearance, and high dosage requirements. To address these limitations, cationic polymeric nanoparticles have been explored, but their development is often limited by toxicity and low transfection efficiency. In this study, we present a novel biocompatible delivery system using a combination of cationic and cholesterol-containing polymers to overcome these issues. The system was formulated into nanocomplexes (NCs) for the delivery of C Peptide (CPep) and miRNA-29b (miR29b). The formulation process involved electrostatic complexation of CPep/miR29b with the polymeric carriers, avoiding harsh conditions or chemical modifications. Native-PAGE, gel retardation, and heparin competition assays confirmed stable complexation. Cell uptake and transfection studies showed efficient delivery of both CPep and miR29b via NCs. In vitro models of oxidative and metabolic stress demonstrated enhanced cell viability with CPep NCs compared to free CPep, with increased glutathione and reduced nitric oxide levels. Similarly, miR29b NCs exhibited potent anti-inflammatory effects compared to free miR29b. This study presents a promising polymer-based carrier system for effective peptide and miRNA delivery through electrostatic interactions alone without any chemical reaction involved to preserve the integrity of the therapeutic.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"173 ","pages":"Article 214284"},"PeriodicalIF":5.5,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143627953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-09DOI: 10.1016/j.bioadv.2025.214282
Shazia Hameed , Saeed Ur Rahman , Kiran Konain , Muhammad Samie , Sajida Farid , Jeevithan Elango , Syed Rashid Habib , Kyung Mi Woo , Praveen R. Arany
The study focuses on developing and evaluating 3D biomimetic fibrous scaffolds to enhance osteoblast differentiation and bone tissue regeneration. Utilizing a synergistic approach, biological and chemical factors were compartmentalized within the fibrous scaffolds through co-axial electrospinning. Bioactive glass (BG) was used for osteo-conductivity, and Bone-Conditioned Medium (BCM) for osteoinduction. The BCM, derived from ovine bone chips, was investigated for its optimal concentration using pre-osteoblast cells. Comprehensive assessment of the scaffolds included physicochemical properties, drug release, cell viability, and osteogenic potential. The scaffold's architecture, confirmed by Scanning electron microscopy (SEM) analysis, effectively emulated the natural extracellular matrix (ECM). Energy Dispersive X-ray Spectroscopy (EDX) and Fourier Transform Infrared Spectroscopy (FTIR) analyses verified the successful integration of BG and BCM, while UV–Vis spectroscopy demonstrated controlled BCM release. Both BG and BCM scaffolds notably enhanced osteoblast differentiation, as evident with Alizarin red staining. The combined use of BG and BCM in scaffolds synergistically promoted osteogenic differentiation and viability of MC3T3-E1 cells. Furthermore, these scaffolds significantly increased the expression of Bone Sialoprotein (BSP), Osteocalcin (OCN), and Runt-related transcription factor 2 (RUNX2) which indicate increase in osteogenic differentiation. This study provides evidence for advanced scaffold systems that can guide cell responses for effective bone tissue regeneration.
{"title":"Advanced 3D biomimetic scaffolds with bioactive glass and bone-conditioned medium for enhanced osteogenesis","authors":"Shazia Hameed , Saeed Ur Rahman , Kiran Konain , Muhammad Samie , Sajida Farid , Jeevithan Elango , Syed Rashid Habib , Kyung Mi Woo , Praveen R. Arany","doi":"10.1016/j.bioadv.2025.214282","DOIUrl":"10.1016/j.bioadv.2025.214282","url":null,"abstract":"<div><div>The study focuses on developing and evaluating 3D biomimetic fibrous scaffolds to enhance osteoblast differentiation and bone tissue regeneration. Utilizing a synergistic approach, biological and chemical factors were compartmentalized within the fibrous scaffolds through co-axial electrospinning. Bioactive glass (BG) was used for osteo-conductivity, and Bone-Conditioned Medium (BCM) for osteoinduction. The BCM, derived from ovine bone chips, was investigated for its optimal concentration using pre-osteoblast cells. Comprehensive assessment of the scaffolds included physicochemical properties, drug release, cell viability, and osteogenic potential. The scaffold's architecture, confirmed by Scanning electron microscopy (SEM) analysis, effectively emulated the natural extracellular matrix (ECM). Energy Dispersive X-ray Spectroscopy (EDX) and Fourier Transform Infrared Spectroscopy (FTIR) analyses verified the successful integration of BG and BCM, while UV–Vis spectroscopy demonstrated controlled BCM release. Both BG and BCM scaffolds notably enhanced osteoblast differentiation, as evident with Alizarin red staining. The combined use of BG and BCM in scaffolds synergistically promoted osteogenic differentiation and viability of MC3T3-E1 cells. Furthermore, these scaffolds significantly increased the expression of Bone Sialoprotein (BSP), Osteocalcin (OCN), and Runt-related transcription factor 2 (RUNX2) which indicate increase in osteogenic differentiation. This study provides evidence for advanced scaffold systems that can guide cell responses for effective bone tissue regeneration.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"173 ","pages":"Article 214282"},"PeriodicalIF":5.5,"publicationDate":"2025-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143609218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-09DOI: 10.1016/j.bioadv.2025.214280
Wenqing Yu , Liwei Hu , Yige Wei , Chengyu Xue , Yunfei Liu , Huixu Xie
Tooth extraction is a common oral surgical procedure that often leads to delayed alveolar socket healing due to the complexity of the oral microenvironment, which can hinder the patient's aesthetic and functional recovery. Effective alveolar socket healing requires a multidisciplinary approach. Recent advancements in materials science and bioengineering have facilitated the development of innovative strategies, with hydrogels emerging as ideal restorative materials for alveolar socket repair due to their superior properties. This review provides an overview of recent advances in hydrogels for alveolar socket healing, focusing on their classification, physical properties (e.g., mechanical strength, swelling behavior, degradation rate, and injectability), biological functions, and applications in relevant animal models. Specifically, the bone-regenerative and antimicrobial properties of hydrogels are highlighted. Furthermore, this review identifies future directions and addresses challenges associated with the clinical application of hydrogels in extraction socket healing.
{"title":"Advances of novel hydrogels in the healing process of alveolar sockets","authors":"Wenqing Yu , Liwei Hu , Yige Wei , Chengyu Xue , Yunfei Liu , Huixu Xie","doi":"10.1016/j.bioadv.2025.214280","DOIUrl":"10.1016/j.bioadv.2025.214280","url":null,"abstract":"<div><div>Tooth extraction is a common oral surgical procedure that often leads to delayed alveolar socket healing due to the complexity of the oral microenvironment, which can hinder the patient's aesthetic and functional recovery. Effective alveolar socket healing requires a multidisciplinary approach. Recent advancements in materials science and bioengineering have facilitated the development of innovative strategies, with hydrogels emerging as ideal restorative materials for alveolar socket repair due to their superior properties. This review provides an overview of recent advances in hydrogels for alveolar socket healing, focusing on their classification, physical properties (e.g., mechanical strength, swelling behavior, degradation rate, and injectability), biological functions, and applications in relevant animal models. Specifically, the bone-regenerative and antimicrobial properties of hydrogels are highlighted. Furthermore, this review identifies future directions and addresses challenges associated with the clinical application of hydrogels in extraction socket healing.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"173 ","pages":"Article 214280"},"PeriodicalIF":5.5,"publicationDate":"2025-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143620799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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