Materials Science & Engineering C-Materials for Biological Applications最新文献_第7页

Synthesis of N-substituted phenothiazine styrene monomers for amphiphilic fluorescence nanoparticles: structure-fluorescence relationship, from AIE to ACQ effect and drugs delivery systems 两亲性荧光纳米粒子n -取代吩噻嗪苯乙烯单体的合成：结构-荧光关系，从AIE到ACQ效应和给药系统。

IF 6 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications

Pub Date : 2025-12-26 DOI: 10.1016/j.bioadv.2025.214684

Zengfang Huang , Xiaotong Zhang , Jinwen Tan , Siying Yang , Yikai Lin , Jinwen Yan , Jinying Yuan , Lei Tao , Mei Tian , Yen Wei

Phenothiazines are highly active and attracting significant attention in various fields including biology and medicine, etc. Herein, several styryl phenothiazine derivatives including VPPA, VPBM, 3PVP and 4PVP were successfully synthesized, and their relationship of functional groups and photophysical properties was investigated in detail. Among them, VPPA demonstrated twisted intramolecular charge transfer (TICT) and aggregation-induced emission (AIE) properties, while 4PVP with phenothiazine-pyridine structure demonstrated aggregation-caused quenching (ACQ) effect and obvious pH sensitivity for targeting potential in various pH environments. In order to explore AIE and ACQ effects, a series of fluorescence performances, molecular electrostatic potential (ESP), stacking models, and quantization calculations were conducted. Subsequently, 3PVP, 4PVP and VPPA were copolymerized by RAFT polymerization with PEGMA for their potential in intracellular imaging and drugs delivery, and the obtained copolymers self-assembled in aqueous solution into fluorescent nanoparticles with about 150 nm with low toxicity and good biocompatibility. Drugs delivery systems of VA-PTX FONs and 4VP-PTX FONs with the anti-tumors drug paclitaxel (PTX) could successfully release PTX with high toxicity to the A549 cells, which respectively decreased the cells viability to 31.84 % and 26.90 %, showing high cells inhibition ratio. The as-prepared drug-delivery nanoparticles have perspective potential in visualizing, bioimaging, medical diagnostics, and drugs track.

吩噻嗪类药物具有很高的活性，在生物、医学等领域受到广泛关注。本文成功合成了几种苯乙烯基吩噻嗪衍生物，包括VPPA、VPBM、3PVP和4PVP，并详细研究了它们的官能基团与光物理性质的关系。其中，VPPA具有分子内扭曲电荷转移（TICT）和聚集诱导发射（AIE）特性，而具有吩噻嗪-吡啶结构的4PVP具有聚集引起猝灭（ACQ）效应，在不同pH环境下对靶向电位具有明显的pH敏感性。为了探索AIE和ACQ效应，进行了一系列荧光性能、分子静电势（ESP）、堆叠模型和量化计算。随后，将3PVP、4PVP和VPPA与PEGMA进行RAFT聚合，利用其在细胞内成像和药物传递方面的潜力，得到的共聚物在水溶液中自组装成约150 nm的荧光纳米颗粒，具有低毒性和良好的生物相容性。VA-PTX FONs和4VP-PTX FONs结合抗肿瘤药物紫杉醇（paclitaxel， PTX）的给药系统可成功释放高毒性的PTX到A549细胞，分别使细胞活力降低到31.84%和26.90%，显示出较高的细胞抑制率。制备的给药纳米颗粒在可视化、生物成像、医学诊断和药物跟踪等方面具有广阔的应用前景。

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引用次数: 0

3D printing of Y-27632 enhanced elastic hydrogel conduits for peripheral nerve repair 3D打印Y-27632增强弹性水凝胶导管修复周围神经。

IF 6 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications

Pub Date : 2025-12-26 DOI: 10.1016/j.bioadv.2025.214679

Wenbi Wu , Haofan Liu , Jiamei Zhang , Yinchu Dong , Min Peng , Qi Zhu , Yi Zhang , Wei Zhao , Li Zhang , Ya Ren , Jinlu Liu , Boya Li , Wentao Li , Yu Hu , Jingzhu Duan , Maling Gou

Nerve guide conduits provide an advanced tool for peripheral nerve repair, but their efficacy remains limited due to suboptimal axon regeneration. Y-27632, a ROCK inhibitor, has the potential to promote axon regeneration and functional restoration. Here, we show a Y-27632 enhanced elastic hydrogel conduit for effective nerve repair. The conduit comprised of gelatin methacryloyl (GelMA)/silk fibroin methacryloyl (SF-MA) hydrogels with Y-27632 loaded poly(lactic-co-glycolic acid) (PLGA)/polyvinyl alcohol (PVA) nanoparticles, is rapidly fabricated by a continuous 3D printing process. The drug Y-27632 can be sustainedly released from the conduits to promote neurite elongation. Meanwhile, the bioactive gelatin/fibroin hydrogels can facilitate Schwann cell adhesion, proliferation, and migration. Moreover, the elastic hydrogel conduit can be surgically sutured with nerve stumps to bridge nerve defects. At 16 weeks post-surgery, this conduit efficiently promotes axon regeneration and remyelination, facilitates muscle re-innervation, and enhances functional recovery in a 12 mm nerve defect model. These findings implicate that elastic hydrogel conduits with Y-27632 release would provide a promising therapeutic strategy for long-gap peripheral nerve defects.

神经引导导管为周围神经修复提供了一种先进的工具，但由于轴突再生不理想，其效果仍然有限。Y-27632是一种ROCK抑制剂，具有促进轴突再生和功能恢复的潜力。在这里，我们展示了Y-27632增强弹性水凝胶导管用于有效的神经修复。该导管由明胶甲基丙烯酰(GelMA)/丝素甲基丙烯酰（SF-MA）水凝胶和Y-27632负载的聚乳酸-羟基乙酸(PLGA)/聚乙烯醇（PVA）纳米颗粒组成，通过连续3D打印工艺快速制造。药物Y-27632可从导管中持续释放，促进神经突伸长。同时，生物活性明胶/丝蛋白水凝胶能够促进雪旺细胞的粘附、增殖和迁移。此外，弹性水凝胶导管可与神经残端手术缝合以桥接神经缺损。术后16周，该导管有效促进轴突再生和髓鞘再生，促进肌肉再神经支配，并增强12mm神经缺损模型的功能恢复。这些发现提示具有Y-27632释放的弹性水凝胶导管将为长间隙周围神经缺损提供有希望的治疗策略。

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引用次数: 0

Combining cartilaginous microtissues primed under altered oxygen environments with melt electrowritten meshes to engineer scaled-up grafts 结合在改变氧环境下启动的软骨微组织与熔体电写网来设计放大的移植物。

IF 6 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications

Pub Date : 2025-12-25 DOI: 10.1016/j.bioadv.2025.214680

Nadia Rodriguez , Inês F. Gonçalves , Tom Hodgkinson , Fergal J. O'Brien , Daniel J. Kelly

Current articular cartilage tissue engineering strategies fail to produce grafts that recapitulate the complex zonal composition and organisation of the native tissue. Developmental engineering strategies might overcome such limitations, leading to the generation of more biomimetic grafts by mimicking key steps of normal tissue development. One such approach leverages the capacity of stem/progenitor cells to self-organise into microtissues or organoids, which can be used as the building blocks of larger grafts. In this study, adult human bone marrow-derived mesenchymal stem/stromal cells (hMSCs) were used to engineer phenotypically distinct hyaline cartilage microtissues for the modular assembly of zonally defined grafts. Altered oxygen (O₂) levels, within the physiological range, were first explored to modulate the microtissue phenotype. Melt electrowritten (MEW) meshes were then used to guide the fusion of such microtissues into scaled-up grafts. Priming hMSC-derived microtissues at oxygen levels representative of different regions of the native tissue supported the development of distinct cartilaginous phenotypes. In addition, short-term exposure to 2 % O₂ significantly enhanced the deposition of glycosaminoglycans compared to exposure to 5 % O₂. Combining such microtissues with a supporting MEW mesh enabled the development of a larger graft with controlled geometry. In conclusion, this study highlights the potential of hMSC-derived cartilage microtissues, primed under altered oxygen environments, as building blocks for the biofabrication of articular cartilage grafts when combined with supporting MEW meshes.

目前的关节软骨组织工程策略无法产生能够再现原生组织复杂的区域组成和组织的移植物。发展工程策略可能克服这些限制，通过模仿正常组织发育的关键步骤，导致产生更多的仿生移植物。其中一种方法是利用干细胞/祖细胞自组织成微组织或类器官的能力，这些微组织或类器官可以用作更大移植物的构建块。在这项研究中，成人骨髓来源的间充质干细胞/基质细胞（hMSCs）被用于设计表型不同的透明软骨微组织，用于分区定义的移植物的模块化组装。改变氧（O2）水平，在生理范围内，首次探索调节显微组织表型。然后使用熔融电写入（MEW）网格来引导这些微组织融合成比例的移植物。在代表原生组织不同区域的氧水平下启动hmsc衍生的微组织支持不同软骨表型的发展。此外，与暴露于5% O2相比，短期暴露于2% O2显著增强了糖胺聚糖的沉积。将这种微组织与支持的MEW网格相结合，可以开发出具有控制几何形状的更大的移植物。总之，本研究强调了在改变氧环境下启动的hmsc衍生软骨微组织的潜力，当与支持的MEW网相结合时，hmsc衍生软骨微组织作为关节软骨移植物生物构建的基石。

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引用次数: 0

Advancement in fabricating precision hemostats using nanocomposites and polymeric systems: From biofunctionality to clinical translation 使用纳米复合材料和聚合物系统制造精密止血器的进展：从生物功能到临床翻译

IF 6 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications

Pub Date : 2025-12-24 DOI: 10.1016/j.bioadv.2025.214683

Ankit Majie, Santanu Ghosh, Bapi Gorain

The ever-increasing challenge of tackling traumatic injuries urges newer hemostatic technologies with rapid hemorrhagic control and improved wound healing to overcome the limitations of conventional gauze and dressings, used for emergency trauma control. With this aim, the current review focuses on nanocomposites, biopolymeric, and synthetic systems, as well as their complex combinations that incorporate different bioactive nanomaterials into either naturally derived or synthetic polymeric matrices. These nanoscale particles are providing an increased surface area for their bioactivity and improved drug-loading capacity. Additionally, advanced polymeric materials, such as hydrogels, gauzes, microspheres, nanofibers, sponges, and foams, are currently loaded with these nanoparticles to enhance their properties, including improved swellability, porosity, and adsorption. These advanced formulations primarily restrict blood flow by providing a physical barrier, followed by the absorption of platelets and RBCs onto their structural scaffold due to the modulation of their surface charge, imparted by various functional groups. Furthermore, highly porous matrices are known to absorb fluid and concentrate blood cells at the wound surface, inducing the formation of a firm and stable blood clot. Consequently, the nanoparticles released from the polymeric matrices induce several molecular effects to accelerate wound closure.

处理创伤性损伤的挑战日益增加，促使新的止血技术能够快速控制出血和改善伤口愈合，以克服用于紧急创伤控制的传统纱布和敷料的局限性。基于这一目的，目前的综述集中在纳米复合材料、生物聚合物和合成系统，以及它们的复杂组合，将不同的生物活性纳米材料纳入天然衍生或合成聚合物基质中。这些纳米级颗粒为其生物活性和改进的载药能力提供了更大的表面积。此外，先进的聚合物材料，如水凝胶、纱布、微球、纳米纤维、海绵和泡沫，目前都装载了这些纳米颗粒，以增强其性能，包括改善膨胀性、孔隙度和吸附性。这些先进的配方主要通过提供物理屏障来限制血液流动，然后通过各种官能团传递的表面电荷的调节，将血小板和红细胞吸收到其结构支架上。此外，已知高多孔基质可以吸收液体并将血细胞集中在伤口表面，诱导形成坚固稳定的血凝块。因此，从聚合物基质中释放的纳米颗粒诱导了几种分子效应来加速伤口愈合。

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引用次数: 0

Nanoparticle-mediated Zn delivery impacts neural protein phosphatase activity 纳米粒子介导的锌递送影响神经蛋白磷酸酶活性。

IF 6 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications

Pub Date : 2025-12-23 DOI: 10.1016/j.bioadv.2025.214675

André Nadais , Carolina Castro , Inês Martins , Diogo Trigo , Cláudia Nunes , Ana Gabriela Henriques , Maria de Lourdes Pereira , Odete A.B. da Cruz e Silva

In recent years, the use of nanoparticles (NPs) in diagnosis and treatment of different disorders has been a matter of intensive research. Due to their physical and chemical properties, zinc oxide nanoparticles (ZnO NP) have been explored in a range of biological applications, including cancer and neurological diseases. Regarding the latter, while some studies report protective effects of ZnO NP in cultured cells and animal models, others indicate that these NPs have a harmful impact on the brain, such as promoting oxidative stress and cell death.

Previous results from our group have suggested beneficial effects for zinc (Zn) cations in both modulating protein aggregation and on Alzheimer's disease (AD) pathology. In this context, the effect of encapsulated Zn as a nanoparticle on protein aggregation and its influence on protein phosphorylation events associated with AD were explored. The results herein presented show that ZnO NP contributed to a decrease in protein aggregation in neuronal cells. However, these NPs were also found to decrease PP1 and PP2A activity, potentially contributing to increased phosphorylation of tau and APP, which are AD pathology hallmarks.

In conclusion, while the use of NPs as a Zn delivery system may offer benefits by reducing aggregate formation, they also appear to induce undesired molecular changes, like those observed in AD. Therefore, a holistic approach should be incorporated as we move forward in this research line, as their effects on distinct cellular processes may be dual edged.

近年来，纳米颗粒（NPs）在不同疾病的诊断和治疗中的应用已成为一个深入研究的问题。氧化锌纳米颗粒（ZnO NP）由于其物理和化学性质，在包括癌症和神经系统疾病在内的一系列生物学应用中得到了探索。对于后者，虽然一些研究报道了ZnO NP在培养细胞和动物模型中的保护作用，但也有研究表明这些NPs对大脑有有害影响，如促进氧化应激和细胞死亡。我们小组先前的研究结果表明，锌（Zn）阳离子在调节蛋白质聚集和阿尔茨海默病（AD）病理方面都有有益的作用。在此背景下，研究人员探讨了锌作为纳米颗粒对蛋白质聚集的影响及其对AD相关蛋白磷酸化事件的影响。结果表明，ZnO NP有助于神经元细胞中蛋白质聚集的减少。然而，这些NPs也被发现降低PP1和PP2A的活性，可能导致tau和APP磷酸化增加，这是AD的病理标志。综上所述，虽然使用NPs作为锌递送系统可能通过减少聚集体的形成而带来好处，但它们也似乎会诱导不希望的分子变化，就像在AD中观察到的那样。因此，当我们在这条研究线上前进时，应该采用整体方法，因为它们对不同细胞过程的影响可能是双刃剑。

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引用次数: 0

A four-pronged approach to unleash the regenerative potential of extracellular vesicles for bone regeneration: A systematic review of in vitro and in vivo studies 释放细胞外囊泡用于骨再生的再生潜力的四管齐下方法：体外和体内研究的系统综述。

IF 6 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications

Pub Date : 2025-12-23 DOI: 10.1016/j.bioadv.2025.214661

Shayan Salehy , Hanieh Nokhbatolfoghahaei , Arash Khojasteh

The clinical application of Extracellular vesicles for bone regeneration faces several key challenges, including low production yield and potency, a lack of standardized isolation and characterization methods, and an incomplete understanding of their regenerative mechanisms, which render their clinical use ineffective. This systematic review addresses these limitations by elucidating regenerative mechanisms and identifying strategies to improve EV potency through an assessment of existing literature. A comprehensive search on MEDLINE, Scopus, and Web of Science yielded 133 articles, 72 (68 in vitro, 27 in vivo) of which met the inclusion criteria based on studies investigating methods to enhance EV bone regenerative potential. Data regarding EV source, affected cells and signaling pathways, study design, animal model, bone defect, administration route, and outcomes (bone formation and vascularization) were extracted and synthesized. Four distinct approaches were identified to improve EV bone regenerative potential at the cell source level: Targeted gene modification, chemical manipulation, Physical stimulation, and Culture-Based Optimization. While all included in vivo studies reported a significant increase in bone formation, none of the studies specified a method to eliminate bias (specifically selection bias, reporting bias, attrition bias, performance bias, and detection bias) in their studies; thus, any interpretations should be made with caution. Future studies should prioritize rigorous methodology centered around EV optimization, including targeted mechanisms for enhanced bone formation, and explore ways to improve clinical translatability. This systematic review was registered with PROSPERO (CDR42024511757).

细胞外囊泡骨再生的临床应用面临着几个关键的挑战，包括低产量和效力，缺乏标准化的分离和表征方法，以及对其再生机制的不完全了解，这使得其临床应用无效。本系统综述通过对现有文献的评估，阐明了再生机制并确定了提高EV效力的策略，从而解决了这些局限性。在MEDLINE、Scopus和Web of Science上进行综合检索，获得133篇文章，其中72篇（68篇体外，27篇体内）符合研究增强EV骨再生潜力方法的纳入标准。提取和合成有关EV来源、受影响细胞和信号通路、研究设计、动物模型、骨缺损、给药途径和结果（骨形成和血管形成）的数据。四种不同的方法可以在细胞源水平上提高EV骨再生潜力：靶向基因修饰、化学操作、物理刺激和基于培养的优化。虽然所有纳入的体内研究都报告了骨形成的显著增加，但没有一项研究规定了在其研究中消除偏倚（特别是选择偏倚、报告偏倚、损耗偏倚、表现偏倚和检测偏倚）的方法；因此，任何解释都应谨慎。未来的研究应优先考虑以EV优化为中心的严谨方法，包括增强骨形成的靶向机制，并探索提高临床可翻译性的方法。该系统评价已在普洛斯彼罗注册（CDR42024511757）。

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引用次数: 0

Sustainable fabrication of biopolymer-based (starch/PVA) nanoscaffolds loaded with green-synthesized CuO nanoparticles from Sargassum wightii (marine macroalgae): Cytocompatibility and antioxidant activity 负载绿色合成的巨藻氧化铜纳米颗粒的生物聚合物基（淀粉/PVA）纳米支架的可持续制备：细胞相容性和抗氧化活性

IF 6 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications

Pub Date : 2025-12-23 DOI: 10.1016/j.bioadv.2025.214660

JinJin Pei , Hanisha Govindaraj , Sivaramakrishnan R , Gopalakrishnan Velliyur Kanniappan , Vijayalakshmi Pandurangan , Selvaraj Jayaraman , Vinoth Kumar Dhayalan , Monica Mironescu , Ion Dan Mironescu , Chella Perumal Palanisamy

This study presents an eco-friendly approach for synthesizing copper oxide nanoparticles (CuO NPs) using the aqueous extract of Sargassum wightii, a brown marine alga, and their integration into starch/polyvinyl alcohol (PVA)-based electrospun nanoscaffolds

. The biosynthesized CuO NPs were characterized by UV–Vis spectroscopy, FTIR, and XRD, confirming their formation, functional groups, and crystalline structure. Scanning electron microscopy revealed uniform distribution of CuO NPs within the nanofibrous matrix, while thermogravimetric analysis (TGA) confirmed enhanced thermal stability of the scaffolds. Dynamic light scattering (DLS) and zeta potential analyses indicated good colloidal stability and nanoscale size distribution. In vitro cytotoxicity studies demonstrated that all samples were non-cytotoxic to 3T3-L1 cells (p > 0.05), supporting their basic biocompatibility. While the scaffolds showed apparent chemical radical-scavenging activity, no functional biological assays were performed; therefore, no claims regarding proliferation or wound-healing efficacy are made. The materials warrant further investigation in future biological models.

本研究提出了一种利用褐藻马尾藻水萃取物合成氧化铜纳米粒子（CuO NPs）的环保方法，并将其整合到淀粉/聚乙烯醇（PVA）基静电纺纳米支架中。通过紫外可见光谱、红外光谱和x射线衍射对合成的CuO纳米粒子进行了表征，确定了它们的形成、官能团和晶体结构。扫描电镜结果显示，纳米纤维基质中CuO纳米粒子分布均匀，热重分析（TGA）证实了支架的热稳定性增强。动态光散射（DLS）和zeta电位分析表明，该材料具有良好的胶体稳定性和纳米级尺寸分布。体外细胞毒性研究表明，所有样品对3T3-L1细胞无细胞毒性（p > 0.05），支持其基本生物相容性。虽然支架具有明显的化学自由基清除活性，但未进行功能性生物学检测；因此，没有关于增殖或伤口愈合功效的声明。这些材料值得在未来的生物模型中进一步研究。

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引用次数: 0

Freeze-dried and imprinted collagen scaffolds in tendon engineering 冻干和印迹胶原蛋白支架在肌腱工程中的应用。

IF 6 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications

Pub Date : 2025-12-23 DOI: 10.1016/j.bioadv.2025.214681

Ignacio Sallent , Lefki Chaniotaki , Dimitrios I. Zeugolis

Collagen type I, as the main component of tendons, is an attractive biomaterial for the development of scaffolds for tendon repair and regeneration. Herein, we review advancements in freeze-drying and soft lithography scaffold fabrication procedures that permit the manufacturing of collagen scaffolds with precise architectural features. We also provide an overview of collagen crosslinking strategies that allow for the development of scaffolds with appropriate stiffness and cytocompatibility. In addition, we appraise the contribution of freeze-dried and imprinted collagen scaffolds in tendon engineering.

I型胶原蛋白作为肌腱的主要成分，是肌腱修复和再生支架发展的重要生物材料。在此，我们回顾了冷冻干燥和软光刻支架制造工艺的进展，这些工艺允许制造具有精确结构特征的胶原蛋白支架。我们还提供了胶原交联策略的概述，该策略允许开发具有适当刚度和细胞相容性的支架。此外，我们还评价了冻干和印迹胶原蛋白支架在肌腱工程中的作用。

引用次数: 0

DLP 3D-printed biomimetic periodontium composite with xenogenic treated dentin matrix modulates periodontal regeneration and inflammatory response DLP 3d打印仿生牙周组织复合材料与异种处理牙本质基质调节牙周再生和炎症反应

IF 6 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications

Pub Date : 2025-12-22 DOI: 10.1016/j.bioadv.2025.214668

Xiaoyu Hu , Tao Qiu , Yuxuan Xiao , Yun Wang , Weihua Guo , Yu Chen

Xenogeneic treated dentin matrix (xTDM) roots serve as a promising donor source for tooth replantation. Nevertheless, the regeneration of the alveolar bone–periodontal ligament (PDL)–cementum complex persists as a challenging task owing to tissue heterogeneity, limited vascularization, and immune-associated complications. In this study, a biomimetic tubular periodontium was fabricated through digital light processing (DLP)-based 3D bioprinting around columnar xTDM roots. The bio-ink was composed of silk fibroin methacrylate (SilMA), dental follicle stem cells (DFCs), and transforming growth factor-β1 (TGF-β1). In a rat subcutaneous model, the DLP Bioprinting Periodontium (DLBPB) enhanced the formation of Sharpey's fiber-like bundles with aligned orientation surrounding the xTDM. To address immune rejection, local FTY720 administration down-regulated TNF-α and IL-1β expression and induced macrophage polarization from the M1 toward the M2 phenotype. Co-delivery of TGF-β1 and FTY720 exerted synergistic immunomodulatory effects. RNA sequencing revealed downregulation of Card9 and inhibition of NF-κB signaling, thereby reducing inflammatory mediator expression. This study proposes a modular strategy that integrates biomimetic scaffold design, stem-cell-based biofabrication, and localized immunomodulation to promote functional periodontal regeneration around xTDM. Therapeutic efficacy was verified in a Beagle dog orthotopic tooth replantation model, suggesting the translational potential of DLPBP for clinical functional tooth restoration.

异种处理牙本质基质（xTDM）根是一种很有前途的牙再植供体来源。然而，由于组织异质性、有限的血管化和免疫相关并发症，牙槽骨-牙周韧带(PDL) -骨质复合体的再生仍然是一项具有挑战性的任务。在这项研究中，通过基于数字光处理（DLP）的3D生物打印技术，在柱状xTDM根周围制造了一个仿生管状牙周组织。该生物墨水由甲基丙烯酸丝素（SilMA）、牙滤泡干细胞（dfc）和转化生长因子-β1 （TGF-β1）组成。在大鼠皮下模型中，DLP生物打印牙周组织（DLBPB）增强了xTDM周围定向排列的Sharpey纤维样束的形成。为了解决免疫排斥，局部给药FTY720下调TNF-α和IL-1β表达，诱导巨噬细胞从M1向M2表型极化。TGF-β1与FTY720共递送具有协同免疫调节作用。RNA测序显示Card9下调，NF-κB信号被抑制，从而降低炎症介质的表达。本研究提出了一种模块化的策略，将仿生支架设计、干细胞生物制造和局部免疫调节结合起来，促进xTDM周围的牙周功能再生。在Beagle犬原位牙再植模型中验证了治疗效果，提示DLPBP在临床功能牙修复中的转化潜力。

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引用次数: 0

Multifunctional POSS-based nanoparticles functionalized with silver, SPIONs, and rhamnolipid for antibacterial applications 多功能poss纳米粒子功能化与银，SPIONs，和鼠李糖脂抗菌应用

IF 6 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications

Pub Date : 2025-12-22 DOI: 10.1016/j.bioadv.2025.214678

Güneş Kibar , Melisa Kafali , Olgu Cagan Ozonuk , Merve Oztas , Berk Usta , Batur Ercan

Nano-engineered materials, particularly those featuring bio-based surface modifications, are emerging as effective tools in combating bacterial infections. In this study, polyhedral oligomeric silsesquioxane (POSS) nanoparticles were functionalized with silver nanoparticles (Ag), superparamagnetic iron oxide nanoparticles (SPIONs), and the biosurfactant rhamnolipid (RL)—either individually or in combination—to evaluate their antibacterial and antibiofilm activities against Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa). The modified nanoparticles exhibited sizes ranging from 127 to 227 nm and demonstrated superparamagnetic behavior, offering potential for magnetic targeting. Among the various formulations, the RL-coated, silver- and SPION-decorated POSS nanoparticles (RSMP) exhibited the highest antibacterial efficacy, reducing S. aureus and P. aeruginosa colony growth by approximately 90 % and 66 %, respectively, at a concentration of 0.01 g/L. RSMP nanoparticles also showed strong biofilm inhibition and had the lowest MIC₅₀ values. Notably, these nanoparticles supported the proliferation of human osteoblasts at concentrations up to 0.05 g/L, indicating favorable cytocompatibility. Overall, RSMP nanoparticles present a promising platform for magnetically targetable antibacterial agents, with potential applications in biomedical fields, particularly for managing orthopedic infections.

纳米工程材料，特别是那些具有生物基表面修饰的材料，正在成为对抗细菌感染的有效工具。在这项研究中，多面体低聚硅氧烷（POSS）纳米粒子与银纳米粒子（Ag）、超顺磁性氧化铁纳米粒子（SPIONs）和生物表面活性剂鼠李糖脂（RL）单独或联合功能化，以评估它们对金黄色葡萄球菌（S. aureus）和铜绿假单胞菌（P. aeruginosa）的抗菌和抗生物膜活性。修饰后的纳米颗粒尺寸在127 ~ 227 nm之间，并表现出超顺磁性，为磁靶向提供了潜力。在不同的配方中，rl包被、银和spion修饰的POSS纳米粒子（RSMP）表现出最高的抗菌效果，在0.01 g/L的浓度下，金黄色葡萄球菌和铜绿假单胞菌的菌落生长分别减少约90%和66%。RSMP纳米颗粒也表现出很强的生物膜抑制作用，并且具有最低的MIC₅0值。值得注意的是，这些纳米颗粒在浓度高达0.05 g/L时支持人成骨细胞的增殖，表明具有良好的细胞相容性。总的来说，RSMP纳米颗粒为磁性靶向抗菌剂提供了一个很有前途的平台，在生物医学领域具有潜在的应用，特别是在骨科感染的治疗方面。

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