Materials Science & Engineering C-Materials for Biological Applications最新文献_第7页

Corrigendum to “PVP-stabilized tungsten oxide nanoparticles: pH sensitive anticancer platform with high cytotoxicity” [Mater. Sci. Eng.: C 108 (2020) 110494] “pvp稳定的氧化钨纳米颗粒：具有高细胞毒性的pH敏感抗癌平台”的勘误表。科学。Eng。[c]; [c]；

IF 5.5 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications

Pub Date : 2024-12-26 DOI: 10.1016/j.bioadv.2024.214159

Anton L. Popov , Bingyuan Han , Artem M. Ermakov , Irina V. Savintseva , Olga N. Ermakova , Nelly R. Popova , Alexander B. Shcherbakov , Taisiya O. Shekunova , Olga S. Ivanova , Daniil A. Kozlov , Alexander E. Baranchikov , Vladimir K. Ivanov

引用次数: 0

Anti-miR21-conjugated DNA nanohydrogel for enhanced cancer therapy 抗mir21结合DNA纳米水凝胶增强癌症治疗。

IF 5.5 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications

Pub Date : 2024-12-24 DOI: 10.1016/j.bioadv.2024.214160

Minhyuk Lee , Jimin Hwang , Youngseo Song , Sungjee Kim , Nokyoung Park

MicroRNAs (miRNAs) are non-coding, endogenous small single-stranded RNA molecules involved in post-transcriptional regulation of gene expression. It has been demonstrated that dysregulation of miRNA plays a major role in tumor formation, proliferation, and metastasis. Therefore, the delivery of anti-miRNA oligonucleotides to block the activity of these oncogenic miRNAs is a high-potential anti-cancer therapy approach. In particular, miRNA-21 (miR-21) can be an excellent target as it is an oncogenic miRNA that is upregulated in various cancers including glioblastoma, breast cancer, and colon cancer. However, anti-miRNAs are unstable in the physiological environment and have low cell membrane permeability, making it difficult to accumulate at certain concentrations to have anti-cancer effects within cancer cells. To overcome these difficulties, we developed anti-miR-21 functionalized DNA hydrogel (amiR-21 Dgel). We confirmed the improved physiological stability of amiR-21 Dgel in vitro, and observed that it blocked up to 96.6 % of miR-21 in HeLa cells, and reduced cell viability down to 77.9 % for 72 h. In particular, RT-qPCR analysis demonstrated that blocking of miR-21 induces increased mRNA expression of the tumor suppressor genes, PTEN and PDCD4 by 6.23- and 6.87-fold, respectively. In addition, the Dgel can act as a drug delivery vehicle, intercalating anticancer drugs such as doxorubicin (Dox) to be delivered into cells. DOX, an anticancer drug, showed a synergistic anticancer effect with amiR-21, which was delivered together. We expect that this approach will be a convenient to optimization and highly effective strategy for anticancer therapy employing antisense miRNAs.

MicroRNAs （miRNAs）是非编码的内源性小单链RNA分子，参与基因表达的转录后调控。研究表明，miRNA的失调在肿瘤的形成、增殖和转移中起着重要作用。因此，递送抗mirna寡核苷酸来阻断这些致癌mirna的活性是一种高潜力的抗癌治疗方法。特别是，miRNA-21 （miR-21）可以成为一个很好的靶点，因为它是一种致癌miRNA，在胶质母细胞瘤、乳腺癌和结肠癌等多种癌症中上调。然而，抗mirna在生理环境中不稳定，细胞膜通透性低，难以在癌细胞内积累到一定浓度，从而产生抗癌作用。为了克服这些困难，我们开发了抗mir -21功能化的DNA水凝胶（amiR-21 Dgel）。我们在体外证实了miR-21凝胶提高了生理稳定性，并观察到它在HeLa细胞中阻断了96.6%的miR-21，并在72小时内将细胞存活率降低到77.9%。特别是，RT-qPCR分析表明，阻断miR-21诱导肿瘤抑制基因PTEN和PDCD4的mRNA表达分别增加了6.23倍和6.87倍。此外，凝胶可以作为药物递送载体，嵌入抗癌药物，如阿霉素（Dox），将其递送到细胞中。DOX是一种抗癌药物，与amiR-21一起给药，显示出协同抗癌作用。我们期望这种方法将为利用反义mirna进行抗癌治疗提供一种方便的优化和高效的策略。

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引用次数: 0

A multifunctional hyaluronic acid-engineered mesoporous nanoreactor with H2O2/O2 self-sufficiency for pH-triggered endo-lysosomal escape and synergetic cancer therapy 具有H2O2/O2自给能力的多功能透明质酸工程介孔纳米反应器，用于ph触发的内溶酶体逃逸和协同癌症治疗。

IF 5.5 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications

Pub Date : 2024-12-23 DOI: 10.1016/j.bioadv.2024.214161

Fei Lu , Moon-Sun Jang , Wei Jiang , Changling Liu , Bo Wang , Jung Hee Lee , Yan Fu , Hong Yu Yang

Monotherapy has poor accuracy and is easily restricted by tumor microenvironment (TME). Remodeling components of the TME to activate multimodal cancer therapy with high precision and efficiency is worth exploring. A multifunctional nanoreactor was fabricated by decorating chlorin e6-modified and PEGylated hyaluronic acid bearing diethylenetriamine-conjugated dihydrolipoic acid on the surface of glucose oxidase (GOx)-loaded hollow mesoporous CuS nanoparticles (labeled as GOx@HCuS@HA). This nanoreactor efficiently targets tumor sites, enhances cellular internalization, and swiftly escapes from endo-lysosomes after intravenous injection. Subsequently, GOx@HCuS@HA was activated in hyaluronidase and H + -rich TME to produce H₂O₂ and gluconic acid through the oxidation of glucose, which not only blocks the energy supply of cancer cells, executing starvation treatment (ST), but also bolsters hydroxyl radicals (•OH)-based chemodynamic therapy (CDT) by Fenton-like reaction between HCuS and H₂O₂. Furthermore, reductive Cu ions could catalyze H₂O₂ to produce O₂ to alleviate the limitation of photodynamic therapy (PDT) for tumor hypoxia. Additionally, the photothermal effect of HCuS under NIR irradiation could increase the temperature of tumor tissues to perform photothermal therapy (PTT). This synergistic antitumor strategy could ultimately achieve precise tumor cell destruction and maintain excellent biosafety. Hence, this nanoreactor offer promising prospects for efficient tumor treatment.

单药治疗准确性差，易受肿瘤微环境（TME）的限制。重构TME组分激活多模态肿瘤治疗的高精度和高效率值得探索。在葡萄糖氧化酶（GOx）负载的中空介孔cu纳米粒子（标记为GOx@HCuS@HA）表面修饰含二乙三胺偶联二氢硫辛酸的氯修饰聚乙二醇透明质酸，制备了多功能纳米反应器。这种纳米反应器有效地靶向肿瘤部位，增强细胞内化，并在静脉注射后迅速从内溶酶体中逃逸。随后，GOx@HCuS@HA在透明质酸酶和富H +的TME中被激活，通过葡萄糖的氧化产生H2O2和葡萄糖酸，不仅阻断癌细胞的能量供应，进行饥饿治疗（ST），还通过hcu和H2O2之间的芬顿样反应支持基于羟基自由基（•OH）的化学动力学治疗（CDT）。此外，还原性Cu离子可以催化H2O2生成O2，减轻光动力治疗（PDT）对肿瘤缺氧的限制。此外，hcu在近红外照射下的光热效应可以提高肿瘤组织的温度，进行光热治疗（PTT）。这种协同抗肿瘤策略最终可以实现精确的肿瘤细胞破坏，并保持良好的生物安全性。因此，这种纳米反应器为有效治疗肿瘤提供了广阔的前景。

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引用次数: 0

Dopamine and alkylated silica functionalized Janus gauze with fluid control capability for rapid hemostasis 多巴胺和烷基化二氧化硅功能化Janus纱布具有快速止血的液体控制能力。

IF 5.5 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications

Pub Date : 2024-12-21 DOI: 10.1016/j.bioadv.2024.214156

Qingheng Wu , Yimeng Cai , Ke Xu , Dan Wu , Xuan Li , Yi Zhao , Denghao Huang , Shuqin Cao , Malcolm Xing , Leixiao Yu , Quan Yuan

Hemorrhage caused by trauma is a global public health issue. While traditional cotton gauze compression is commonly used for hemostasis, its efficacy is limited in severe hemorrhage cases. Herein, we developed a gauze with Janus wettability (JW-G). Its surface was sequentially dip-coated with hydrophilic polydopamine and then spray-coated with hydrophobic silicon dioxide nanoparticles. Benefiting from the asymmetric surface wettability, the obtained JW-G could control blood flow direction and permeation speed. JW-G's hydrophobic surface concentrates blood cells and facilitate faster clot formation in vitro. Meanwhile, the silicon dioxide nanoparticles could also activate coagulation factor XII. Therefore, the JW-G greatly accelerates the hemostasis. In vivo bleeding model further demonstrated JW-G's good performance in reducing bleeding time and blood loss compared with commercial hemostatic gauze. The present strategy to develop hemostatic gauze offers a promising solution for reversing the adverse outcomes of severe traumatic hemorrhage.

外伤引起的出血是一个全球性的公共卫生问题。传统的棉纱布压迫止血常用，但对大出血患者的止血效果有限。在此，我们开发了一种具有Janus润湿性（JW-G）的纱布。其表面依次浸涂亲水性聚多巴胺，然后喷涂疏水性二氧化硅纳米颗粒。利用表面的不对称润湿性，所得的JW-G可以控制血液流动方向和渗透速度。JW-G的疏水表面浓缩血细胞，促进体外更快的凝块形成。同时，二氧化硅纳米颗粒还能激活凝血因子XII。因此，JW-G能大大促进止血。体内出血模型进一步证明了JW-G与市售止血纱布相比在减少出血时间和出血量方面的良好性能。目前开发止血纱布的策略为扭转严重创伤性出血的不良后果提供了一个有希望的解决方案。

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引用次数: 0

Photoactive broad-spectrum dressings with antimicrobial and antitumoral properties 具有抗菌和抗肿瘤特性的光活性广谱敷料。

IF 5.5 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications

Pub Date : 2024-12-20 DOI: 10.1016/j.bioadv.2024.214158

Gonzalo E. Cagnetta , Sol R. Martínez , Luis E. Ibarra , Ana Wendel , Rodrigo E. Palacios , Carlos A. Chesta , María Lorena Gómez

In this work the development of photoactive dressings (PAD) with dual purpose, is presented. These PAD can be used for the topical treatment of persistent infections caused by fungi and bacteria and are also applicable in light antitumor therapy for carcinoma.

The synthesized PAD were designed employing conjugated polymer nanoparticles (CPN) doped with platinum porphyrin which serve as polymerization photoinitiators and photosensitizers for the production of reactive oxygen species (ROS). This approach led to the synthesis of N-vinyl-2-pyrrolidone (NVP) hydrogels co-polymerized with [2-(methacryloyloxy)ethyl] trimethylammonium chloride (METAC). NVP and METAC were selected to impart a good biocompatibility with eukaryotic cell lines and antimicrobial properties, respectively. The combination of METAC with an efficient photogeneration of ROS by doped CPN resulted in a material with outstanding antimicrobial features. These dressings are capable of producing an aseptic environment upon irradiation and demonstrates a bacteriostatic profile in dark conditions. Additionally, the dressings fulfill critical requirements for topical applications, providing protection and acting as a barrier, with appropriate mechanical and swelling properties; as well as adequate water vapor transmission rates. The synthesized PAD have been shown to be biocompatible and non-toxic to erythrocytes and HaCaT cell line.

PAD demonstrated efficacy in eliminating microbes such as fungi and bacteria. The underlying light-induced killing mechanism involved protein photooxidation, which amplified the effects of METAC mechanism that disrupt cellular membranes. Furthermore, in vitro studies using carcinoma cell lines displayed a complete cell eradication using a relatively low light dose (36 J/cm² at 395 nm). These promising results reveal also the potential of PAD in the treatment of skin cancer.

本文介绍了两用光活性敷料（PAD）的发展情况。这些PAD可用于真菌和细菌引起的持续性感染的局部治疗，也可用于癌症的轻度抗肿瘤治疗。采用掺杂铂卟啉的共轭聚合物纳米颗粒（CPN）作为聚合光引发剂和光敏剂制备活性氧（ROS）。该方法合成了n -乙烯基-2-吡咯烷酮（NVP）与[2-（甲基丙烯酰氧基）乙基]三甲基氯化铵（METAC）共聚合的水凝胶。选择NVP和METAC分别具有良好的真核细胞系生物相容性和抗菌性能。METAC与掺杂CPN的ROS的高效光生成相结合，产生了具有出色抗菌特性的材料。这些敷料能够在照射后产生无菌环境，并在黑暗条件下显示出抑菌性。此外，敷料满足局部应用的关键要求，提供保护和作为屏障，具有适当的机械和肿胀特性；以及足够的水蒸气透过率。合成的PAD对红细胞和HaCaT细胞系具有生物相容性和无毒性。PAD在消除真菌和细菌等微生物方面表现出功效。潜在的光诱导杀伤机制涉及蛋白质光氧化，这放大了破坏细胞膜的METAC机制的作用。此外，使用癌细胞系进行的体外研究显示，使用相对较低的光剂量（36j /cm2, 395 nm）可以完全根除癌细胞。这些有希望的结果也揭示了PAD治疗皮肤癌的潜力。

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引用次数: 0

The gelatin sponge loaded with curcumin coating exhibits a synergistic effect of hemostasis, anti-inflammatory, and anti-scarring 姜黄素包覆的明胶海绵具有止血、抗炎和抗疤痕的协同作用。

IF 5.5 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications

Pub Date : 2024-12-20 DOI: 10.1016/j.bioadv.2024.214155

Xiaoxue Tan , Hongzhong Xi , Peng Xue , Jinxin Cao , M.A. Yarmolenko , Xin Liu , Xiaohong Jiang

Postoperative wound healing has been extensively studied and well-documented. Gelatin sponges are commonly used in surgeries for blood absorption. If these sponges can also release drugs with anti-scarring and anti-inflammatory effects, they would significantly enhance wound healing. In this study, we investigated for the first time the application of curcumin films on the surface of gelatin sponges with high hemostatic efficiency using the Electron Beam Deposition (EBD) method. The structure of curcumin was analyzed using ¹H NMR, FT-IR and XPS techniques. We examined the influence of the film on the sponge's absorption capacity and the impact of the sponge on drug release kinetics. Results showed that the presence of the curcumin film did not compromise the sponge's hemostatic ability. Additionally, compared to a flat substrate, the curcumin film on this highly porous substrate facilitated better curcumin release. Further experiments, including cytotoxicity tests, live/dead double staining, western blotting, and a scar model in mice, demonstrated that gelatin sponges with curcumin films exhibit a synergistic effect, combining anti-scarring, anti-inflammatory, and hemostatic properties.

术后伤口愈合已被广泛研究和充分记录。明胶海绵通常用于外科手术中的血液吸收。如果这些海绵还能释放具有抗疤痕和抗炎作用的药物，它们将显著促进伤口愈合。本研究首次采用电子束沉积法（EBD）研究了姜黄素薄膜在明胶海绵表面的高效止血作用。采用1H NMR、FT-IR和XPS技术对姜黄素的结构进行了分析。我们考察了薄膜对海绵吸收能力的影响以及海绵对药物释放动力学的影响。结果表明，姜黄素膜的存在不影响海绵的止血能力。此外，与平坦的基底相比，这种高度多孔的基底上的姜黄素膜促进了姜黄素的更好释放。进一步的实验，包括细胞毒性试验、活/死双染色、免疫印迹和小鼠疤痕模型，表明明胶海绵与姜黄素膜具有协同作用，具有抗疤痕、抗炎和止血的特性。

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引用次数: 0

Non-antibiotic dependent photothermal antibacterial hemostatic MXene hydrogel for infectious wounds healing 非抗生素依赖光热抗菌止血MXene水凝胶用于感染性伤口愈合。

IF 5.5 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications

Pub Date : 2024-12-17 DOI: 10.1016/j.bioadv.2024.214157

Xingyu Zhou , Wendi Ma , Junhui Jiang , Junbo Dang , Ruifu Lv , Hongbo Wang , Minna Ma , Dahui Sun , Mei Zhang

On account of the existence of antibiotic resistance, the wound healing of pathogenic infection is still a challenge in modern society. A desirable wound dressing should own the abilities of adhesiveness, hemostasis and good mechanical property, meanwhile the property of eliminating bacteria without side effects is also highly needed. In this work, we established a kind of hydrogel based on carboxymethyl cellulose-graft-tyramine (CMC-Ty) and MXene (Ti₃C₂T_x) through employing H₂O₂/HRP (horseradish peroxidase) as the initiator, then the as-prepared hydrogel (named CMC-Ty/MXene) was immersed in tannic acid (TA) solution, and this TA-treated hydrogel was called CMC-Ty/MXene+TA. By employing TA as the multi-functional H-bond provider, the adhesiveness, hemostatic ability, mechanical property and bactericidal performance of the hydrogel was enhanced. And MXene in this system exerted benign photothermal antimicrobial performance, it was able to transform near-infrared (NIR) light into heat, then the bacteria would be physically damaged (thermal destruction) due to the hyperthermy, hence the antibacterial effect of which will not be restricted by antibiotic resistance. The temperature of the hydrogel in the experimental group can be increased by 25 °C after irradiation by 808 nm NIR light for 10 min, and the bactericidal efficiency against both E. coli and S. aureus reached >99 %. In vivo tests demonstrated that with the assistance of NIR irradiation, the hydrogel can distinctly accelerate the S. aureus infected wound closure. We envisage that this non-antibiotic dependent multifunctional photothermal hydrogel can provide a promise for bacteria-invaded wound healing.

由于抗生素耐药性的存在，致病性感染的伤口愈合在现代社会仍然是一个挑战。一种理想的创面敷料应具有粘附性、止血性和良好的力学性能，同时还应具有除菌无副作用的性能。本研究以H2O2/辣根过氧化物酶（HRP）为引发剂，建立了一种羧甲基纤维素-接枝酪胺（CMC-Ty）和MXene （Ti3C2Tx）为基础的水凝胶，将制备的水凝胶（命名为CMC-Ty/MXene）浸入单宁酸（TA）溶液中，经TA处理后的水凝胶称为CMC-Ty/MXene+TA。利用TA作为多功能氢键提供者，提高了水凝胶的黏附性、止血能力、力学性能和杀菌性能。该体系中的MXene具有良好的光热抗菌性能，它能够将近红外（NIR）光转化为热，然后细菌会因高温而受到物理损伤（热破坏），因此其抗菌效果不会受到抗生素耐药性的限制。实验组水凝胶经808 nm近红外光照射10 min后，温度可提高25℃，对大肠杆菌和金黄色葡萄球菌的杀菌效率均达到99.9%。体内实验表明，在近红外照射的辅助下，水凝胶可以明显加速金黄色葡萄球菌感染的伤口愈合。我们设想这种不依赖抗生素的多功能光热水凝胶可以为细菌入侵的伤口愈合提供希望。

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引用次数: 0

Strategies for the design of biomimetic cell-penetrating peptides using AI-driven in silico tools for drug delivery 使用人工智能驱动的硅工具设计仿生细胞穿透肽的策略用于药物输送。

IF 5.5 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications

Pub Date : 2024-12-16 DOI: 10.1016/j.bioadv.2024.214153

Rebecca Sutcliffe , Ciaran P.A. Doherty , Hugh P. Morgan , Nicholas J. Dunne , Helen O. McCarthy

Cell-penetrating peptides (CPP) have gained rapid attention over the last 25 years; this is attributed to their versatility, customisation, and ‘Trojan horse’ delivery that evades the immune system. However, the current CPP rational design process is limited, as it requires several rounds of peptide synthesis, prediction and wet-lab validation, which is expensive, time-consuming and requires extensive knowledge in peptide chemistry. Artificial intelligence (AI) has emerged as a promising alternative which can augment the design process, for example by determining physiochemical characteristics, secondary structure, solvent accessibility, disorder and flexibility, as well as predicting in vivo behaviour such as toxicity and peptidase degradation. Other more recent tools utilise supervised machine learning (ML) to predict the penetrative ability of an amino acid sequence. The use of AI in the CPP design process has the potential to reduce development costs and increase the chances of success with respect to delivery. This review provides a survey of in silico tools and AI platforms which can be utilised in the design process, and the key features that should be taken into consideration when designing next generation CPPs.

细胞穿透肽（CPP）在过去的25年里得到了迅速的关注；这要归功于它们的多功能性、定制性，以及避开免疫系统的“特洛伊木马”式递送。然而，目前的CPP合理设计过程是有限的，因为它需要多轮肽合成，预测和湿实验室验证，这是昂贵的，耗时的，并且需要广泛的肽化学知识。人工智能（AI）已经成为一种有前途的替代方案，可以增强设计过程，例如通过确定物理化学特性、二级结构、溶剂可及性、无序性和灵活性，以及预测体内行为，如毒性和肽酶降解。其他最近的工具利用监督机器学习（ML）来预测氨基酸序列的渗透能力。在CPP设计过程中使用人工智能有可能降低开发成本，并增加交付成功的机会。本综述提供了可用于设计过程的计算机工具和人工智能平台的调查，以及在设计下一代CPPs时应考虑的关键特征。

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引用次数: 0

Macrophage and osteosarcoma cell crosstalk is dependent on oxygen tension and 3D culture 巨噬细胞和骨肉瘤细胞的串扰依赖于氧张力和三维培养。

IF 5.5 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications

Pub Date : 2024-12-16 DOI: 10.1016/j.bioadv.2024.214154

Katherine H. Griffin , Isabel S. Sagheb , Thomas P. Coonan , Fernando A. Fierro , R. Lor Randall , J. Kent Leach

Osteosarcoma (OS), the most common form of primary bone cancer in young adults, has had no improvements in clinical outcomes in 50 years. This highlights a critical need to advance mechanistic understanding of OS to further therapeutic discovery, which will only be possible with accurate models of the disease. Compared to traditional monolayer studies and preclinical models, in vitro models that better replicate the three-dimensional (3D) bone marrow microenvironment will facilitate methodical investigations of the events and factors that drive OS progression. Herein, we use fibrin-alginate interpenetrating network (FA IPN) hydrogels to model the hematological bone marrow environment. We interrogated the effects of oxygen tension, 3D culture, and macrophage phenotype on OS behavior and specifically examine the immunomodulatory crosstalk between OS and macrophages. We observe that OS is more sensitive to oxygen tension when cultured in 3D. Specifically, both highly and less metastatic OS exhibit decreased changes in DNA content over time in 3D, but then demonstrate diverging behaviors in heterotypic culture with macrophages. OS response to macrophages differs as a function of metastatic potential, where highly metastatic OS shows increased immunosuppression that varies with oxygen tension but relies on direct coculture conditions. To our knowledge, this is among the first work to report the effects of 3D culture on the interplay between OS and macrophages in a coculture microenvironment. Together, these data introduce FA IPNs as a promising platform for cancer research and emphasize the importance of novel models for the mechanistic study of OS.

骨肉瘤（OS）是年轻人中最常见的原发性骨癌，50年来在临床结果上没有任何改善。这凸显了迫切需要推进对OS的机制理解，以进一步发现治疗方法，这只有通过准确的疾病模型才有可能实现。与传统的单层研究和临床前模型相比，体外模型更好地复制了三维（3D）骨髓微环境，将有助于系统地研究驱动OS进展的事件和因素。在这里，我们使用纤维蛋白-海藻酸互穿网络（FA IPN）水凝胶来模拟血液骨髓环境。我们研究了氧张力、3D培养和巨噬细胞表型对OS行为的影响，并专门研究了OS和巨噬细胞之间的免疫调节串扰。我们观察到OS在3D培养时对氧张力更敏感。具体来说，高转移性和低转移性OS在3D中随着时间的推移都表现出DNA含量的变化减少，但随后在巨噬细胞异型培养中表现出不同的行为。OS对巨噬细胞的反应因转移潜能的不同而不同，其中高度转移的OS表现出随氧张力变化而增加的免疫抑制，但依赖于直接共培养条件。据我们所知，这是首次报道3D培养对共培养微环境中OS和巨噬细胞之间相互作用的影响。总之，这些数据介绍了FA ipn作为癌症研究的一个有前途的平台，并强调了新模型对OS机制研究的重要性。

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引用次数: 0

Biomimetic gradient hydrogel with fibroblast spheroids for full-thickness skin regeneration 具有成纤维细胞球体的仿生梯度水凝胶用于全层皮肤再生。

IF 5.5 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications

Pub Date : 2024-12-16 DOI: 10.1016/j.bioadv.2024.214152

Mina Kwon , Yuhan Lee , Ki Su Kim

Hydrogel-based scaffolds have been widely investigated for their use in tissue engineering to accelerate tissue regeneration. However, replicating the physiological microenvironments of tissues with appropriate biological cues remains challenging. Recent advances in gradient hydrogels have transformed tissue-engineering research by providing precise structures that mimic the extracellular matrix of natural tissues. Unlike conventional homogeneously structured hydrogels, gradient hydrogels provide a better bio-mimicking microenvironment for combined cell therapies in chronic wound treatment by regulating various cell behaviors, such as proliferation, migration, and differentiation. Here, we present the integration of L929 mouse fibroblast spheroids into gradient hydrogels to mimic the dermal stiffness microenvironment and we investigated their impact on full-thickness skin regeneration. A stiffness gradient was achieved by modulating the concentration of methacrylated hyaluronic acid (HA-MA) with varying degrees of methacrylation, using a dual-syringe pump system. The encapsulation of L929 spheroids with gradient hydrogel facilitated skin cell organization in a hierarchically ordered configuration, leading to full-thickness wound healing that was 1.53 times faster than the untreated group in a rat model. This study provides a method for investigating the potential role of gradient hydrogels in various tissue engineering and regeneration applications.

水凝胶基支架在组织工程中加速组织再生的应用已被广泛研究。然而，用适当的生物线索复制组织的生理微环境仍然具有挑战性。梯度水凝胶的最新进展通过提供模拟自然组织细胞外基质的精确结构，改变了组织工程研究。与传统的均匀结构水凝胶不同，梯度水凝胶通过调节各种细胞行为，如增殖、迁移和分化，为慢性伤口治疗中的联合细胞治疗提供了更好的生物模拟微环境。在这里，我们将L929小鼠成纤维细胞球体整合到梯度水凝胶中以模拟皮肤硬度微环境，并研究了它们对全层皮肤再生的影响。采用双注射泵系统，通过调节甲基丙烯酸化透明质酸（HA-MA）不同甲基丙烯酸化程度的浓度，实现了硬度梯度。在大鼠模型中，梯度水凝胶包封L929球体促进皮肤细胞分层有序组织，导致全层创面愈合速度比未处理组快1.53倍。本研究为研究梯度水凝胶在各种组织工程和再生应用中的潜在作用提供了一种方法。

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引用次数: 0