Materials Science & Engineering C-Materials for Biological Applications最新文献

英文中文

An adhesive, antibacterial hydrogel wound dressing fabricated by dopamine-grafted oxidized sodium alginate and methacrylated carboxymethyl chitosan incorporated with Cu(II) complex

IF 5.5 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications

Pub Date : 2025-02-06 DOI: 10.1016/j.bioadv.2025.214217

Lei Wang , Huainian Wang , Haoming Dang , Baolong Niu , Hong Yan , Ruijie Guo , Huifang Wang , Pucha Zhou

Effective wound dressings play an important role in preventing infections and promoting wound healing. Most polysaccharide-based hydrogel dressings have the drawbacks of weak tissue adhesion and poor antibacterial properties. Herein, a multifunctional dopamine-grafted oxidized sodium alginate-methacrylated carboxymethyl chitosan/gallic acid‑copper(II) complex (OD-CM/GA-Cu^II_UV) hydrogel was fabricated through Schiff base bonds and photo-crosslinked polymerization between dopamine-grafted oxidized sodium alginate (OSA-DA) and methacrylated carboxymethyl chitosan (CMC-MA), with the integration of gallic acid‑copper(II) complexes (GA-Cu^II). The double cross-linked network and mussel-inspired adhesion mechanism endowed the hydrogel with attractive physicochemical properties, including excellent self-healing properties, pH-responsive biodegradability, robust toughness, and a maximum adhesion strength of 15.06 kPa. Moreover, the composite hydrogel exhibited an antibacterial ratio of > 99 % against Escherichia coli and Staphylococcus aureus, as well as good antioxidant activity. The MTT assay showed that the cell viability of the composite hydrogel reached > 85 %. The in vivo full-thickness skin defect healing assays in rats demonstrated that the composite hydrogel remarkably accelerated wound repair by attenuating the inflammatory response and promoting epithelial tissue remodeling. Therefore, this novel multifunctional hydrogel has potential applications in biomedical wound dressing.

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引用次数: 0

Silanized acrylic graphene oxide nanocomposite reinforced mechanically tunable GelMA/HAMA printable bio-ink for adipose-derived stem cells differentiated mature smooth muscle cells

IF 5.5 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications

Pub Date : 2025-02-06 DOI: 10.1016/j.bioadv.2025.214226

Pavanchandh Atturu , Su-Shin Lee , Po-Chih Chang , Kevin Chiou , Chih-Kuang Wang

Smooth muscle cells (SMCs) phenotype has successfully conserved in the 3D printable GH-ASG bio-inks composed of silanized acrylic graphene oxide nanosheets as a crosslinker (APStriol@GO) comprising of 3-acryloyloxypropyl silanetriol (APStriol) and graphene oxide (GO) reinforced in the hybrid hydrogel consist of methacrylated gelatin (GelMA) and methacrylated hyaluronic acid (HAMA) to develop a photocurable hybrid novel bio-ink (GelMA/HAMA/APStriol@GO) as a component for rabbit adipose-derived stem cells (rADSCs) differentiated SMCs inducing functionalized material in situ. Hybrid GH-ASG hydrogels were evaluated for various physiochemical parameters and chemical modifications. The GH-ASG4 (GelMA/HAMA/APStriol@GO-1 %) bioink exhibited optimal reactive oxygen species scavenging potential, and hemostasis was shown to enhance the viability of rADSCs. Additionally, the morphology and nucleus count for differentiated SMCs were analyzed employing TRAP staining. Moreover, the contractile SMCs phenotype was determined at the transcript level by implementing quantitative RT-PCR using SMCs-specific gene markers (α-SMA and SM-MHC). The protein level of gene expression was assessed through Immunocytochemistry and western blot analysis using SMC-specific antibodies (α-SMA and SM-MHC). GH-ASG4 bio-ink was used for 3D printed tubular and disk scaffold fabrication through extrusion bioprinting with improved biocompatibility, processibility, and higher cell proliferation throughout scaffolds to mimic the SMCs extracellular matrix, crucial for smooth muscle regeneration.

{"title":"Silanized acrylic graphene oxide nanocomposite reinforced mechanically tunable GelMA/HAMA printable bio-ink for adipose-derived stem cells differentiated mature smooth muscle cells","authors":"Pavanchandh Atturu , Su-Shin Lee , Po-Chih Chang , Kevin Chiou , Chih-Kuang Wang","doi":"10.1016/j.bioadv.2025.214226","DOIUrl":"10.1016/j.bioadv.2025.214226","url":null,"abstract":"<div><div>Smooth muscle cells (SMCs) phenotype has successfully conserved in the 3D printable GH-ASG bio-inks composed of silanized acrylic graphene oxide nanosheets as a crosslinker (APStriol@GO) comprising of 3-acryloyloxypropyl silanetriol (APStriol) and graphene oxide (GO) reinforced in the hybrid hydrogel consist of methacrylated gelatin (GelMA) and methacrylated hyaluronic acid (HAMA) to develop a photocurable hybrid novel bio-ink (GelMA/HAMA/APStriol@GO) as a component for rabbit adipose-derived stem cells (rADSCs) differentiated SMCs inducing functionalized material in situ. Hybrid GH-ASG hydrogels were evaluated for various physiochemical parameters and chemical modifications. The GH-ASG4 (GelMA/HAMA/APStriol@GO-1 %) bioink exhibited optimal reactive oxygen species scavenging potential, and hemostasis was shown to enhance the viability of rADSCs. Additionally, the morphology and nucleus count for differentiated SMCs were analyzed employing TRAP staining. Moreover, the contractile SMCs phenotype was determined at the transcript level by implementing quantitative RT-PCR using SMCs-specific gene markers (α-SMA and SM-MHC). The protein level of gene expression was assessed through Immunocytochemistry and western blot analysis using SMC-specific antibodies (α-SMA and SM-MHC). GH-ASG4 bio-ink was used for 3D printed tubular and disk scaffold fabrication through extrusion bioprinting with improved biocompatibility, processibility, and higher cell proliferation throughout scaffolds to mimic the SMCs extracellular matrix, crucial for smooth muscle regeneration.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"171 ","pages":"Article 214226"},"PeriodicalIF":5.5,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143444943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advanced nanomicelles for targeted glioblastoma multiforme therapy

IF 5.5 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications

Pub Date : 2025-02-04 DOI: 10.1016/j.bioadv.2025.214221

P. Chithra, Dhiraj Bhatia, Raghu Solanki

Glioblastoma multiforme (GBM) is the most aggressive and malignant primary brain tumor, classified as grade IV by the WHO. Despite standard treatments like surgical resection, radiotherapy and chemotherapy (i.e. temozolomide), GBM's prognosis remains poor due to its heterogeneity, recurrence and the impermeability of the blood-brain barrier (BBB). The exact cause of GBM is unclear with potential factors including genetic predisposition and ionizing radiation. Innovative approaches such as nanomicelles-nanoscale, self-assembled structures made from lipids and amphiphilic polymers show promise for GBM therapy. These nanocarriers enhance drug solubility and stability, enabling targeted delivery of therapeutic agents across the BBB. This review explores the synthesis strategies, characterization and applications of nanomicelles in GBM treatment. Nanomicelles improve the delivery of both hydrophobic and hydrophilic drugs and provide non-invasive delivery options. By offering site-specific targeting, biocompatibility, and stability, nanomicelles can potentially overcome the limitations of current GBM therapies. This review highlights recent advancements in the use of nanomicelles for delivering therapeutic agents and nucleic acids addressing the critical need for advanced treatments to improve GBM patient outcomes.

{"title":"Advanced nanomicelles for targeted glioblastoma multiforme therapy","authors":"P. Chithra, Dhiraj Bhatia, Raghu Solanki","doi":"10.1016/j.bioadv.2025.214221","DOIUrl":"10.1016/j.bioadv.2025.214221","url":null,"abstract":"<div><div>Glioblastoma multiforme (GBM) is the most aggressive and malignant primary brain tumor, classified as grade IV by the WHO. Despite standard treatments like surgical resection, radiotherapy and chemotherapy (i.e. temozolomide), GBM's prognosis remains poor due to its heterogeneity, recurrence and the impermeability of the blood-brain barrier (BBB). The exact cause of GBM is unclear with potential factors including genetic predisposition and ionizing radiation. Innovative approaches such as nanomicelles-nanoscale, self-assembled structures made from lipids and amphiphilic polymers show promise for GBM therapy. These nanocarriers enhance drug solubility and stability, enabling targeted delivery of therapeutic agents across the BBB. This review explores the synthesis strategies, characterization and applications of nanomicelles in GBM treatment. Nanomicelles improve the delivery of both hydrophobic and hydrophilic drugs and provide non-invasive delivery options. By offering site-specific targeting, biocompatibility, and stability, nanomicelles can potentially overcome the limitations of current GBM therapies. This review highlights recent advancements in the use of nanomicelles for delivering therapeutic agents and nucleic acids addressing the critical need for advanced treatments to improve GBM patient outcomes.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"170 ","pages":"Article 214221"},"PeriodicalIF":5.5,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143231141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrasound stimulated piezoelectric antibacterial silk composite films guiding differentiation of mesenchymal stem cells

IF 5.5 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications

Pub Date : 2025-02-03 DOI: 10.1016/j.bioadv.2025.214218

Namrata Tiwari , Akshay Joshi , Ritu Das , Davinder Singh Lall , Kammari Suresh Chary , Neetu Singh

Smart materials for tissue engineering have been in extensive use for few decades now. This work delves into the exploration of ultrasound-stimulated piezoelectric and antibacterial silk-based composite films as a pioneering strategy to guide the differentiation of human mesenchymal stem cells into osteogenic lineage without the application of any exogenous growth factors. The study evaluates the biocompatibility and antibacterial attributes of these films, which incorporates Barium Titanate nanoparticles (BTNPs) along with Zinc Oxide nanoparticles for obtaining high piezo modulated stimuli response and antibacterial properties. Further, to enhance the piezoelectric capability, a novel calcium doped Barium Titanate (BCTs) nanoparticles were synthesized and incorporated in silk based films with ZnO. The choice of using calcium as a doping material allows to increase its piezoelectric potential and retain its biocompatibility. The results reveal that, under the influence of ultrasound stimulation, these composite films respond to mechanical cues like low frequency ultrasound stimulations to facilitate lineage-specific differentiation of the seeded human mesenchymal stem cells. Ultrasound stimulations being wireless avoid complicated wired electric circuits and are also known to activate calcium channels in the cells which aids osteogenesis. Significantly, our findings exhibit the profound potential of these films to exploit the piezoelectric properties of BCTs, effectively enhancing the differentiation trajectories of stem cells. Furthermore, their demonstrated antibacterial capacities underscore their pivotal role in infection prevention, an important facet in the domains of tissue engineering and medical implantation. This study strongly suggests the utility of ultrasound-stimulated silk-based composite films in advancing the frontiers of regenerative medicine and tissue engineering.

{"title":"Ultrasound stimulated piezoelectric antibacterial silk composite films guiding differentiation of mesenchymal stem cells","authors":"Namrata Tiwari , Akshay Joshi , Ritu Das , Davinder Singh Lall , Kammari Suresh Chary , Neetu Singh","doi":"10.1016/j.bioadv.2025.214218","DOIUrl":"10.1016/j.bioadv.2025.214218","url":null,"abstract":"<div><div>Smart materials for tissue engineering have been in extensive use for few decades now. This work delves into the exploration of ultrasound-stimulated piezoelectric and antibacterial silk-based composite films as a pioneering strategy to guide the differentiation of human mesenchymal stem cells into osteogenic lineage without the application of any exogenous growth factors. The study evaluates the biocompatibility and antibacterial attributes of these films, which incorporates Barium Titanate nanoparticles (BTNPs) along with Zinc Oxide nanoparticles for obtaining high piezo modulated stimuli response and antibacterial properties. Further, to enhance the piezoelectric capability, a novel calcium doped Barium Titanate (BCTs) nanoparticles were synthesized and incorporated in silk based films with ZnO. The choice of using calcium as a doping material allows to increase its piezoelectric potential and retain its biocompatibility. The results reveal that, under the influence of ultrasound stimulation, these composite films respond to mechanical cues like low frequency ultrasound stimulations to facilitate lineage-specific differentiation of the seeded human mesenchymal stem cells. Ultrasound stimulations being wireless avoid complicated wired electric circuits and are also known to activate calcium channels in the cells which aids osteogenesis. Significantly, our findings exhibit the profound potential of these films to exploit the piezoelectric properties of BCTs, effectively enhancing the differentiation trajectories of stem cells. Furthermore, their demonstrated antibacterial capacities underscore their pivotal role in infection prevention, an important facet in the domains of tissue engineering and medical implantation. This study strongly suggests the utility of ultrasound-stimulated silk-based composite films in advancing the frontiers of regenerative medicine and tissue engineering.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"170 ","pages":"Article 214218"},"PeriodicalIF":5.5,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143349351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retraction notice to “Folic acid conjugated curcumin loaded biopolymeric gum acacia microsphere for triple negative breast cancer therapy in invitro and invivo model” [Mater. Sci. Eng.: C 95 (2019) 8997] 叶酸共轭姜黄素负载生物聚合物阿拉伯胶微球用于三阴性乳腺癌治疗的体外和体内模型》的撤稿通知 [Mater. Sci. Eng.: C 95 (2019) 8997]。

IF 5.5 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications

Pub Date : 2025-02-01 DOI: 10.1016/j.bioadv.2024.214077

Kunal Pal , Shubham Roy , Pravat Kumar Parida , Ananya Dutta , Souravi Bardhan , Sukhen Das , Kuladip Jana , Parimal Karmakar

引用次数: 0

Corrigendum to “Optimising a self-assembling peptide hydrogel as a Matrigel alternative for 3-dimensional mammary epithelial cell culture” [Biomater. Adv. volume 160, (2024) 213847] 对 "优化自组装肽水凝胶作为三维乳腺上皮细胞培养的 Matrigel 替代品 "的更正[Biomater. Adv. 第 160 卷，(2024) 213847]。

IF 5.5 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications

Pub Date : 2025-02-01 DOI: 10.1016/j.bioadv.2024.214111

Eliana Lingard , Siyuan Dong , Anna Hoyle , Ellen Appleton , Alis Hales , Eldhose Skaria , Craig Lawless , Isobel Taylor-Hearn , Simon Saadati , Qixun Chu , Aline F. Miller , Marco Domingos , Alberto Saiani , Joe Swift , Andrew P. Gilmore

引用次数: 0

High-density lipoprotein-like nanoparticles with cationic cholesterol derivatives for siRNA delivery

IF 5.5 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications

Pub Date : 2025-01-31 DOI: 10.1016/j.bioadv.2025.214202

Aliaksei Ihnatsyeu-Kachan , Olga Sharko , Andrei Bekish , Anastasiia Saichuk , Victoriya Zhogla , Viktar Abashkin , Egor Ulashchik , Dzmitry Shcharbin , Wilfried Le Goff , Anatol Kontush , Isabelle Guillas , Vadim Shmanai , Sehoon Kim

A new approach to siRNA delivery using high-density lipoprotein-like nanoparticles (HDL NPs) was investigated, incorporating oligoamine and cholesterol-derived cationic lipids (CLs) to associate siRNA with the carrier. Newly designed or commercially available compounds, including GL67 and 3-

β

-[N-(N′,N′-dimethylaminoethane)-carbamoyl]cholesterol (DC-Cholesterol), were tested for siRNA binding, cytotoxicity, and siRNA cellular uptake. GL67 emerged as the most promising CL for siRNA delivery via HDL NPs. While it contributed to substantial siRNA uptake and cytosolic delivery in HepG2 cells, gene silencing remained limited, indicating a need for further optimization. Despite this, the study highlights the potential of positively charged cholesterol derivatives for siRNA delivery using HDL NPs. An analysis of the relationship between CL head group structure and HDL NPs' siRNA binding efficiency and cytotoxicity showed that factors such as oligoamine molecule conjugation site, linker type, amine group ethylation, and alkyl chain length between amine groups are crucial for optimizing CL design. Furthermore, the phospholipid environment surrounding CLs significantly influences HDL NPs' performance, particularly in siRNA cellular uptake. The study also revealed that intracellular siRNA trafficking varies by cell type, emphasizing the importance of customizing HDL NP formulations for specific cells. These insights are important for designing more effective HDL NPs for siRNA therapeutic delivery.

{"title":"High-density lipoprotein-like nanoparticles with cationic cholesterol derivatives for siRNA delivery","authors":"Aliaksei Ihnatsyeu-Kachan , Olga Sharko , Andrei Bekish , Anastasiia Saichuk , Victoriya Zhogla , Viktar Abashkin , Egor Ulashchik , Dzmitry Shcharbin , Wilfried Le Goff , Anatol Kontush , Isabelle Guillas , Vadim Shmanai , Sehoon Kim","doi":"10.1016/j.bioadv.2025.214202","DOIUrl":"10.1016/j.bioadv.2025.214202","url":null,"abstract":"<div><div>A new approach to siRNA delivery using high-density lipoprotein-like nanoparticles (HDL NPs) was investigated, incorporating oligoamine and cholesterol-derived cationic lipids (CLs) to associate siRNA with the carrier. Newly designed or commercially available compounds, including GL67 and 3-<span><math><mi>β</mi></math></span>-[N-(N′,<em>N</em>′-dimethylaminoethane)-carbamoyl]cholesterol (DC-Cholesterol), were tested for siRNA binding, cytotoxicity, and siRNA cellular uptake. GL67 emerged as the most promising CL for siRNA delivery via HDL NPs. While it contributed to substantial siRNA uptake and cytosolic delivery in HepG2 cells, gene silencing remained limited, indicating a need for further optimization. Despite this, the study highlights the potential of positively charged cholesterol derivatives for siRNA delivery using HDL NPs. An analysis of the relationship between CL head group structure and HDL NPs' siRNA binding efficiency and cytotoxicity showed that factors such as oligoamine molecule conjugation site, linker type, amine group ethylation, and alkyl chain length between amine groups are crucial for optimizing CL design. Furthermore, the phospholipid environment surrounding CLs significantly influences HDL NPs' performance, particularly in siRNA cellular uptake. The study also revealed that intracellular siRNA trafficking varies by cell type, emphasizing the importance of customizing HDL NP formulations for specific cells. These insights are important for designing more effective HDL NPs for siRNA therapeutic delivery.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"170 ","pages":"Article 214202"},"PeriodicalIF":5.5,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143350905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Injectable myocardium-derived hydrogels with SDF-1α releasing for cardiac repair

IF 5.5 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications

Pub Date : 2025-01-30 DOI: 10.1016/j.bioadv.2025.214203

Jiazhu Xu , Jacob Brown , Rubia Shaik , Luis Soto-Garcia , Jun Liao , Kytai Nguyen , Ge Zhang , Yi Hong

Myocardial infarction (MI) is a predominant cause of morbidity and mortality globally. Therapeutic chemokines, such as stromal cell-derived factor 1α (SDF-1α), present a promising opportunity to treat the profibrotic remodeling post-MI if they can be delivered effectively to the injured tissue. However, direct injection of SDF-1α or physical entrapment in a hydrogel has shown limited efficacy. Here, we developed a sustained-release system consisting of SDF-1α loaded poly(lactic-co-glycolic acid) nanoparticles (PLGA NPs) and an injectable porcine cardiac decellularized extracellular matrix (cdECM) hydrogel. This system demonstrated a sustained release of SDF-1α over four weeks while there is one week release for SDF-1α directly encapsulated in the cdECM hydrogel during in vitro testing. The incorporation of PLGA NPs into the cdECM hydrogel significantly enhanced its mechanical properties, increasing the Young's modulus from 561 ± 228 kPa to 1007 ± 2 kPa and the maximum compressive strength from 639 ± 42 kPa to 1014 ± 101 kPa. This nanocomposite hydrogel showed good cell compatibility after 7 days of culture with H9C2 cells, while the released SDF-1α retained its bioactivity, as evidenced by its chemotactic effects in vitro. Furthermore, in vivo studies further highlighted its significant ability to promote angiogenesis in the infarcted area and improve cardiac function after intramyocardial injection. These results demonstrated the therapeutic potential of combining local release of SDF-1α with the cdECM hydrogel for MI treatment.

{"title":"Injectable myocardium-derived hydrogels with SDF-1α releasing for cardiac repair","authors":"Jiazhu Xu , Jacob Brown , Rubia Shaik , Luis Soto-Garcia , Jun Liao , Kytai Nguyen , Ge Zhang , Yi Hong","doi":"10.1016/j.bioadv.2025.214203","DOIUrl":"10.1016/j.bioadv.2025.214203","url":null,"abstract":"<div><div>Myocardial infarction (MI) is a predominant cause of morbidity and mortality globally. Therapeutic chemokines, such as stromal cell-derived factor 1α (SDF-1α), present a promising opportunity to treat the profibrotic remodeling post-MI if they can be delivered effectively to the injured tissue. However, direct injection of SDF-1α or physical entrapment in a hydrogel has shown limited efficacy. Here, we developed a sustained-release system consisting of SDF-1α loaded poly(lactic-<em>co</em>-glycolic acid) nanoparticles (PLGA NPs) and an injectable porcine cardiac decellularized extracellular matrix (cdECM) hydrogel. This system demonstrated a sustained release of SDF-1α over four weeks while there is one week release for SDF-1α directly encapsulated in the cdECM hydrogel during <em>in vitro</em> testing. The incorporation of PLGA NPs into the cdECM hydrogel significantly enhanced its mechanical properties, increasing the Young's modulus from 561 ± 228 kPa to 1007 ± 2 kPa and the maximum compressive strength from 639 ± 42 kPa to 1014 ± 101 kPa. This nanocomposite hydrogel showed good cell compatibility after 7 days of culture with H9C2 cells, while the released SDF-1α retained its bioactivity, as evidenced by its chemotactic effects <em>in vitro.</em> Furthermore, <em>in vivo</em> studies further highlighted its significant ability to promote angiogenesis in the infarcted area and improve cardiac function after intramyocardial injection. These results demonstrated the therapeutic potential of combining local release of SDF-1α with the cdECM hydrogel for MI treatment.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"170 ","pages":"Article 214203"},"PeriodicalIF":5.5,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143230934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

3D nanofibrous frameworks with on-demand engineered gray and white matters for reconstructing the injured spinal cord

IF 5.5 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications

Pub Date : 2025-01-30 DOI: 10.1016/j.bioadv.2025.214200

André F. Girão , Nathalie Barroca , Yasmina Hernández-Martín , António Completo , Paula A.A.P. Marques , María C. Serrano

Spinal cord injury (SCI) is a disruptive and heterogeneous medical condition affecting millions of patients worldwide. Due to the absence of medical treatments to effectively restore the lost sensorimotor and autonomic functions, there is an ongoing pursuit of scaffolds aiming to bridge the injured spinal area. Herein, a novel electrospinning modality to construct 3D nanofibrous frameworks (NFFs) in accordance with distinct spinal cord microenvironments is used to engineer a biomimetic hemicord. This scaffolding concept gravitates around the possibility of customizing NFFs with on-demand engineered gray and white matters to replicate the native spinal cytoarchitecture. In particular, a 3D reduced graphene oxide-based fibrous-porous system is developed to imitate the gray matter, while a 3D polycaprolactone (PCL)-chitosan nanofibrous network combined with PCL-graphene microfibers intends to mimic the white matter. The scaffolding components are tested in vitro with embryonic neural progenitor cells, integrated into the biomimetic NFF, and then tested in vivo in paralyzed rats with cervical hemisection. After 4 months of implantation, the scaffold generates both neuroprotective (e.g., limited infiltration of vimentin⁺ and ED1⁺ cells) and neuroregenerative (e.g., presence of new blood vessels and neurites) features accompanied with promising signs of forelimb function recovery.

{"title":"3D nanofibrous frameworks with on-demand engineered gray and white matters for reconstructing the injured spinal cord","authors":"André F. Girão , Nathalie Barroca , Yasmina Hernández-Martín , António Completo , Paula A.A.P. Marques , María C. Serrano","doi":"10.1016/j.bioadv.2025.214200","DOIUrl":"10.1016/j.bioadv.2025.214200","url":null,"abstract":"<div><div>Spinal cord injury (SCI) is a disruptive and heterogeneous medical condition affecting millions of patients worldwide. Due to the absence of medical treatments to effectively restore the lost sensorimotor and autonomic functions, there is an ongoing pursuit of scaffolds aiming to bridge the injured spinal area. Herein, a novel electrospinning modality to construct 3D nanofibrous frameworks (NFFs) in accordance with distinct spinal cord microenvironments is used to engineer a biomimetic hemicord. This scaffolding concept gravitates around the possibility of customizing NFFs with on-demand engineered gray and white matters to replicate the native spinal cytoarchitecture. In particular, a 3D reduced graphene oxide-based fibrous-porous system is developed to imitate the gray matter, while a 3D polycaprolactone (PCL)-chitosan nanofibrous network combined with PCL-graphene microfibers intends to mimic the white matter. The scaffolding components are tested <em>in vitro</em> with embryonic neural progenitor cells, integrated into the biomimetic NFF, and then tested <em>in vivo</em> in paralyzed rats with cervical hemisection. After 4 months of implantation, the scaffold generates both neuroprotective (<em>e.g.</em>, limited infiltration of vimentin<sup>+</sup> and ED1<sup>+</sup> cells) and neuroregenerative (<em>e.g.</em>, presence of new blood vessels and neurites) features accompanied with promising signs of forelimb function recovery.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"170 ","pages":"Article 214200"},"PeriodicalIF":5.5,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143132802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

3D pancreatic ductal adenocarcinoma desmoplastic model: Glycolysis facilitating stemness via ITGAV-PI3K-AKT-YAP1

IF 5.5 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications

Pub Date : 2025-01-29 DOI: 10.1016/j.bioadv.2025.214215

Xiaoqi Guan , Di Wu , Hongyu Zhu , Biwen Zhu , Zhen Wang , Haowei Xing , Xue Zhang , Jiashuai Yan , Yibing Guo , Yuhua Lu

The distinctive desmoplastic tumor microenvironment (TME) of pancreatic ductal adenocarcinoma (PDAC) is crucial in determining the stemness of tumor cells. And the conventional two-dimensional (2D) culture does not adequately mimic the TME. Therefore, a three-dimensional (3D) PDAC desmoplastic model was constructed using GelMA and HAMA, which provides benefits in terms of simulating both the main components (COL and HA) and the crosslinking of the extracellular matrix. We found that the 3D PDAC desmoplastic model upregulated the expression of the markers for stemness (NANOG and OCT4) and glycolysis (HK2 and GLUT2), and elevated the level of glycolysis, including increased glucose consumption and lactic acid production. Additionally, YAP1 played a crucial role in promoting glycolysis, which boosted stemness. Furthermore, RNA sequencing (RNA-seq) was employed to explore the underlying mechanisms associated with stemness within the 3D desmoplastic model. Subsequent KEGG pathway analysis indicated the activation of the PI3K-AKT signaling pathway, providing insights into the molecular processes at play. Using bioinformatics, qRT-PCR and western blot, we proposed that ITGAV-PI3K-AKT-YAP1 axis may account for the glycolysis mediated the stemness. Collectively, the 3D desmoplastic model may serve as a new platform for understanding the underlying mechanism by which the TME induces stemness.

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