Arfianti Arfianti, U. Ulfah, Leopold Sampetua Hutabarat, Agnes Ivana G, A. Budiarti, N. Sahara, Nicko Pisceski Kusika Saputra
Background : Mesenchymal stem cell (MSC) has great potential as therapies due its ability to regenerate tissue damage and promote tissue homeostasis. Preconditioning of MSC in low oxygen concentration has been shown to affect the therapeutic potential of these cells. This study aimed to compare the characteristic and secretion of trophic factors of MSCs cultured under hypoxia and normoxia. Methods : MSCs were isolated from Wharton ' s jelly of human umbilical cord (UC) tissue by explant method and characterized by fl ow cytometry. Following 24 h of CoCl 2 -induced hypoxic culture, the viability and metabolic activity of MSC were analyzed by trypan blue exclusion test and methyl thiazolyl tetrazolium (MTT) assay, respectively. The secretion of hepatocyte growth factor (HGF) and vascular endothelial growth factor (VEGF) was assessed in conditioned medium using enzyme-linked immunosorbent assay (ELISA) method. Results : Flow cytometry analysis showed > 99% of the population of MSCs cells were positive for CD73 and CD90 and > 62% were positive for CD105. While the cell viability of MSC was not affected by hypoxic cultured condition, the metabolic activity rate of these cells was decreased under hypoxic conditioning. In line with reduced metabolic activity, hypoxic human UC-derived MSC produced less HGF than normoxic counterpart. Compared to normoxic MSC, hypoxic preconditioned MSC secreted higher level of VEGF in the conditioned medium (p < 0.05). Conclusions : Hypoxia decreased the metabolic activity of MSCs associated with the modulation of HGF and VEGF secretions. It is suggested that hypoxia may also affect the therapeutic capacity of MSC cells.
{"title":"Hipoxia modulates the secretion of growth factors in of human umbilical cord-derived mesenchymal stem cells","authors":"Arfianti Arfianti, U. Ulfah, Leopold Sampetua Hutabarat, Agnes Ivana G, A. Budiarti, N. Sahara, Nicko Pisceski Kusika Saputra","doi":"10.37796/2211-8039.1416","DOIUrl":"https://doi.org/10.37796/2211-8039.1416","url":null,"abstract":"Background : Mesenchymal stem cell (MSC) has great potential as therapies due its ability to regenerate tissue damage and promote tissue homeostasis. Preconditioning of MSC in low oxygen concentration has been shown to affect the therapeutic potential of these cells. This study aimed to compare the characteristic and secretion of trophic factors of MSCs cultured under hypoxia and normoxia. Methods : MSCs were isolated from Wharton ' s jelly of human umbilical cord (UC) tissue by explant method and characterized by fl ow cytometry. Following 24 h of CoCl 2 -induced hypoxic culture, the viability and metabolic activity of MSC were analyzed by trypan blue exclusion test and methyl thiazolyl tetrazolium (MTT) assay, respectively. The secretion of hepatocyte growth factor (HGF) and vascular endothelial growth factor (VEGF) was assessed in conditioned medium using enzyme-linked immunosorbent assay (ELISA) method. Results : Flow cytometry analysis showed > 99% of the population of MSCs cells were positive for CD73 and CD90 and > 62% were positive for CD105. While the cell viability of MSC was not affected by hypoxic cultured condition, the metabolic activity rate of these cells was decreased under hypoxic conditioning. In line with reduced metabolic activity, hypoxic human UC-derived MSC produced less HGF than normoxic counterpart. Compared to normoxic MSC, hypoxic preconditioned MSC secreted higher level of VEGF in the conditioned medium (p < 0.05). Conclusions : Hypoxia decreased the metabolic activity of MSCs associated with the modulation of HGF and VEGF secretions. It is suggested that hypoxia may also affect the therapeutic capacity of MSC cells.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45122123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kishan Prasad H.L., Nimmy Maria Dasan, A. Kishan, S. Kumari N
Introduction and Aim: The most frequent gynaecological neoplasm in women of reproductive age is leiomyoma (Fibroid). They are hormone-dependent tumours, and estrogen will promote their development. It has been found that estrogen levels and cholesterol levels are inversely associated. Few studies have shown no relation between lipid profile and leiomyoma, but few studies suggested a positive correlation. The lipid profile of patients with uterine fibroids (the case group) and women without fibroids (the control group) is compared in the current study. Materials and Methods: The case-control study included fifty subjects between 20 to 40 years. Twenty-five serum samples collected from women without evidence of leiomyoma were considered the control group. Twenty-five cases were admitted with various symptoms, diagnosed and confirmed as leiomyoma on histopathology. Results: There was a substantial difference in Total Cholesterol (TC) and Low-Density Lipoprotein cholesterol (LDL-C) levels between patients with leiomyoma and healthy people. With a p-value of 0.04, the TC, LDL-C level in leiomyoma patients was substantially lower than that of healthy individuals in the general population. The levels of triglycerides, High-Density Lipoprotein cholesterol (HDL-C), and Very Low-Density Lipoprotein cholesterol (VLDL-C) were comparable across the case and control groups, though. Conclusion: According to the study's findings, patients with leiomyoma have lower serum TC and LDL-C levels than healthy controls. This could be linked to patients with leiomyomas having higher estrogen levels. As a result, lipid profile variables like TC and LDL-C may be accurate indicators of leiomyoma. These findings point to the possibility of blood plasma lipid profiling for the non-invasive diagnosis of fibroids.
{"title":"Lipid profile in patients with leiomyoma-A case-control study","authors":"Kishan Prasad H.L., Nimmy Maria Dasan, A. Kishan, S. Kumari N","doi":"10.51248/.v43i3.1443","DOIUrl":"https://doi.org/10.51248/.v43i3.1443","url":null,"abstract":"Introduction and Aim: The most frequent gynaecological neoplasm in women of reproductive age is leiomyoma (Fibroid). They are hormone-dependent tumours, and estrogen will promote their development. It has been found that estrogen levels and cholesterol levels are inversely associated. Few studies have shown no relation between lipid profile and leiomyoma, but few studies suggested a positive correlation. The lipid profile of patients with uterine fibroids (the case group) and women without fibroids (the control group) is compared in the current study.\u0000 \u0000Materials and Methods: The case-control study included fifty subjects between 20 to 40 years. Twenty-five serum samples collected from women without evidence of leiomyoma were considered the control group. Twenty-five cases were admitted with various symptoms, diagnosed and confirmed as leiomyoma on histopathology.\u0000 \u0000Results: There was a substantial difference in Total Cholesterol (TC) and Low-Density Lipoprotein cholesterol (LDL-C) levels between patients with leiomyoma and healthy people. With a p-value of 0.04, the TC, LDL-C level in leiomyoma patients was substantially lower than that of healthy individuals in the general population. The levels of triglycerides, High-Density Lipoprotein cholesterol (HDL-C), and Very Low-Density Lipoprotein cholesterol (VLDL-C) were comparable across the case and control groups, though.\u0000 \u0000Conclusion: According to the study's findings, patients with leiomyoma have lower serum TC and LDL-C levels than healthy controls. This could be linked to patients with leiomyomas having higher estrogen levels. As a result, lipid profile variables like TC and LDL-C may be accurate indicators of leiomyoma. These findings point to the possibility of blood plasma lipid profiling for the non-invasive diagnosis of fibroids.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47560798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bimal K. Agrawal, Manu Mathew, S. Kalia, Aditi Tongar
An elderly female presented with abdominal pain, vomiting and easy fatigability. Her sensorium gradually declined and became comatose. She was on Methotrexate, folic acid and prednisolone for her rheumatoid arthritis. The neurological examination and neuroimaging did not contribute in making the diagnosis. It is challenging to diagnose a metabolic cause of coma. Once diagnosis is established, the management becomes easier. On detailed investigation, she was found to have severe hypercalcemia with corrected serum calcium level being 18.2 mg%. Serum vitamin D level was also high, 150 ng/ml. On probing for detailed history, it was revealed that she had been taking a weekly dose of 60,000 IU of vitamin D for nearly 5 years. In recent times, association of various diseases has been reported with vitamin D deficiency. Vitamin D supplementation seems justified in this patient considering her age and the fact that she had rheumatoid arthritis; she was also taking corticosteroid. The recommended dose of vitamin D has been 400–800 IU/day. However, it is often prescribed at a dose of 60,000 IU/week, and sometimes patients self-medicate. There are no guidelines available for prescribing vitamin D at such a strength. Though safety of vitamin D has been established, vitamin D toxicity can occur sporadically with serious consequences. This was managed with intravenous fluids and diuretics. The patient also required subcutaneous calcitonin and low dose of corticosteroid, in addition to a session of haemodialysis for her hypercalcemia. The patient’s sensorium gradually improved as her calcium level returned to normalcy.
{"title":"Hypervitaminosis D induced hypercalcemia leading to coma: A case report","authors":"Bimal K. Agrawal, Manu Mathew, S. Kalia, Aditi Tongar","doi":"10.51248/.v43i3.3192","DOIUrl":"https://doi.org/10.51248/.v43i3.3192","url":null,"abstract":"An elderly female presented with abdominal pain, vomiting and easy fatigability. Her sensorium gradually declined and became comatose. She was on Methotrexate, folic acid and prednisolone for her rheumatoid arthritis. The neurological examination and neuroimaging did not contribute in making the diagnosis. It is challenging to diagnose a metabolic cause of coma. Once diagnosis is established, the management becomes easier. On detailed investigation, she was found to have severe hypercalcemia with corrected serum calcium level being 18.2 mg%. Serum vitamin D level was also high, 150 ng/ml. On probing for detailed history, it was revealed that she had been taking a weekly dose of 60,000 IU of vitamin D for nearly 5 years. In recent times, association of various diseases has been reported with vitamin D deficiency. Vitamin D supplementation seems justified in this patient considering her age and the fact that she had rheumatoid arthritis; she was also taking corticosteroid. The recommended dose of vitamin D has been 400–800 IU/day. However, it is often prescribed at a dose of 60,000 IU/week, and sometimes patients self-medicate. There are no guidelines available for prescribing vitamin D at such a strength. Though safety of vitamin D has been established, vitamin D toxicity can occur sporadically with serious consequences. This was managed with intravenous fluids and diuretics. The patient also required subcutaneous calcitonin and low dose of corticosteroid, in addition to a session of haemodialysis for her hypercalcemia. The patient’s sensorium gradually improved as her calcium level returned to normalcy.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":"1 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41320009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Introduction and Aim: The disease caused by Corona virus (Covid-19) has become a public health importance in recent times as its human-to-human transmission is faster when compared to other pandemic of recent times. Since the outbreak of Covid-19, epidemiology and clinical characteristics of the disease including spread of the disease and mortality have been reported extensively. Little is known about Covid 19 impact on cognition; hence the present was undertaken. The study aimed at assessing the cognitive status in patients admitted for infection with corona and to determine the correlation between cognitive status and blood pressure in the same patients. Materials and Methods: The study is a cross-sectional study that included 300 adults of both genders admitted in the tertiary care hospital with RTPCR positive for Covid-19. A cognition failure questionnaire was used to record the patient’s response and also BP was recorded. Results: For analysis Descriptive statistics like mean and Standard Deviation, and to find association between parameters - Spearman correlation, Mann Whitney U Test were used. Analysis was done using SPSS version 22. Even though there was no significant association seen between Overall cognitive scores, forgetfulness scores, and false triggering scores with gender, there was significant association seen between overall cognitive scores and false triggering scores with age. Applying spearman correlation with respect to overall cognitive failure with duration of hospital stay showed statistically significance*(p<0.001) and between cognitive failure and diastolic blood pressure showed statistical significance with respect to overall cognition. Conclusion: Infection with Covid-19 has an effect on cognition. Duration of hospital stay has an impact on cognition with associated blood pressure changes in the Covid-19 disease.
{"title":"Assessment of the cognitive status of Covid -19 patients and its correlation to blood pressure","authors":"Mamatha S.D., Priya S.A., S. L., Shivakumar K.M., Smitha Malenahalli Chandrashekarappa","doi":"10.51248/.v43i3.2264","DOIUrl":"https://doi.org/10.51248/.v43i3.2264","url":null,"abstract":"Introduction and Aim: The disease caused by Corona virus (Covid-19) has become a public health importance in recent times as its human-to-human transmission is faster when compared to other pandemic of recent times. Since the outbreak of Covid-19, epidemiology and clinical characteristics of the disease including spread of the disease and mortality have been reported extensively. Little is known about Covid 19 impact on cognition; hence the present was undertaken. The study aimed at assessing the cognitive status in patients admitted for infection with corona and to determine the correlation between cognitive status and blood pressure in the same patients. \u0000 \u0000Materials and Methods: The study is a cross-sectional study that included 300 adults of both genders admitted in the tertiary care hospital with RTPCR positive for Covid-19. A cognition failure questionnaire was used to record the patient’s response and also BP was recorded. \u0000 \u0000Results: For analysis Descriptive statistics like mean and Standard Deviation, and to find association between parameters - Spearman correlation, Mann Whitney U Test were used. Analysis was done using SPSS version 22. Even though there was no significant association seen between Overall cognitive scores, forgetfulness scores, and false triggering scores with gender, there was significant association seen between overall cognitive scores and false triggering scores with age. Applying spearman correlation with respect to overall cognitive failure with duration of hospital stay showed statistically significance*(p<0.001) and between cognitive failure and diastolic blood pressure showed statistical significance with respect to overall cognition. \u0000 \u0000Conclusion: Infection with Covid-19 has an effect on cognition. Duration of hospital stay has an impact on cognition with associated blood pressure changes in the Covid-19 disease.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46557930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Priyanka Madhavan, Shruti D Nayak, S. Prabhu, M. Jose
Jaw lesions such as radicular cyst, odontogenic keratocyst, osteomyelitis associated with Actinomyces have been reported by many authors in the literature. Actinomycotic colonization in developmental cysts like GOC is extremely rare. So far in the literature search, the previous reports have not described actinomyces co-existing with GOC to our knowledge. Herein, we report a rare case report of GOC coexisting with actinomycotic colonies and pathogenesis of actinomycosis associated with odontogenic cyst.
{"title":"Glandular odontogenic cyst coexisting with actinomycosis: A rare case report","authors":"Priyanka Madhavan, Shruti D Nayak, S. Prabhu, M. Jose","doi":"10.51248/.v43i3.2325","DOIUrl":"https://doi.org/10.51248/.v43i3.2325","url":null,"abstract":"Jaw lesions such as radicular cyst, odontogenic keratocyst, osteomyelitis associated with Actinomyces have been reported by many authors in the literature. Actinomycotic colonization in developmental cysts like GOC is extremely rare. So far in the literature search, the previous reports have not described actinomyces co-existing with GOC to our knowledge. Herein, we report a rare case report of GOC coexisting with actinomycotic colonies and pathogenesis of actinomycosis associated with odontogenic cyst.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48542570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Introduction and Aim: The sinonasal region is affected by different types of neoplastic and non-neoplastic conditions. This study attempts to highlight our experience with these lesions and to categorize them. Methods: A retrospective study was conducted in our hospitals and data including history, clinical findings, and histopathological diagnosis was collected from medical records of 187 patients who visited the outpatient department with nasal, paranasal sinus or nasopharyngeal lesions from 2007 to 2020. Results: A total of 187 patients were included. The commonest site involved was the nasal cavity in 104 (55.61%) patients, followed by maxillary sinus in 26 (13.9%) patients and nasopharynx in 16 (8.56%) patients. The majority of the cases were benign tumours (41.71%), followed by malignancy (35.29%). Inverted papilloma was the commonest benign tumour (13.9%); squamous cell carcinoma was the most frequent type of malignancy (12.3%). Conclusion: The overlapping features of nasal masses make diagnosis a challenge, the role of histopathology is indispensable. Early diagnosis and treatment are essential for better clinical management, which will ultimately enhance healthcare.
{"title":"Sinonasal lesions – Case analysis at a tertiary care centre in coastal India","authors":"M. Khadilkar, Deviprasad Dosemane, U. Khadilkar","doi":"10.51248/.v43i3.2591","DOIUrl":"https://doi.org/10.51248/.v43i3.2591","url":null,"abstract":"Introduction and Aim: The sinonasal region is affected by different types of neoplastic and non-neoplastic conditions. This study attempts to highlight our experience with these lesions and to categorize them.\u0000 \u0000Methods: A retrospective study was conducted in our hospitals and data including history, clinical findings, and histopathological diagnosis was collected from medical records of 187 patients who visited the outpatient department with nasal, paranasal sinus or nasopharyngeal lesions from 2007 to 2020.\u0000 \u0000Results: A total of 187 patients were included. The commonest site involved was the nasal cavity in 104 (55.61%) patients, followed by maxillary sinus in 26 (13.9%) patients and nasopharynx in 16 (8.56%) patients. The majority of the cases were benign tumours (41.71%), followed by malignancy (35.29%). Inverted papilloma was the commonest benign tumour (13.9%); squamous cell carcinoma was the most frequent type of malignancy (12.3%).\u0000 \u0000Conclusion: The overlapping features of nasal masses make diagnosis a challenge, the role of histopathology is indispensable. Early diagnosis and treatment are essential for better clinical management, which will ultimately enhance healthcare.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47237147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jeeva S., Kesavarajan S., M. A, S. S, Meenakaumari R., R. Sudalaimani
Introduction and Aim: Anaemia is the most common deficiency disorder among people of all age groups, known as Paandu in the Siddha system of medicine. The test drug siddha poly mineral formulation Lavana Dravagam mentioned in Siddha literature kannukamiyam ennum vaithiya segaram, has been used for pandu (anaemia). Dravagam is a form of internal medicine, processed by distillation method and is also referred as Pugai neer, Shakthi neer, Dravaga neer. The study aimed to evaluate the haematinic activity and efficacy of poly mineral Siddha formulation Lavana Dravagam against phenylhydrazine induced anaemic albino Wistar rat model. Materials and Methods: The animals were selected and divided into four groups (I, II, III, and IV) of six rats (n=6) each. Anaemia was induced by an oral administration of phenylhydrazine (single dose of 10 mg/kg per oral for 8 days). Group I served as normal control and Group II received standard drug hematinic syrup in suspension form at dose 2 mL/kg. Groups III, IV received the formulated oral indiffusible mixture of Lavana Dravagam at a dose of 0.02ml to 0.03ml/kg respectively. RBC, Hb, PCV, MCV, MCH, were analyzed as indices of anaemia. The mean corpuscular volume, mean corpuscular Hb, and mean corpuscular Hb concentration were calculated. Results: From the literature evidence, acute toxicity evaluation and pharmacological studies, the drug Lavana Dravagam is found out to have hematinic activity. This study reveals that there is significant (P<0.05) increase in RBC count, Hb level, and PCV by administering phenylhydrazine within one week of treatment. Conclusion: It could be concluded that the drug Lavana dravagam will have promising effects in the management of anaemia (Paandu).
{"title":"Pharmacological evaluation for haematinic activity of Siddha formulation Lavana Dravagam in rat model","authors":"Jeeva S., Kesavarajan S., M. A, S. S, Meenakaumari R., R. Sudalaimani","doi":"10.51248/.v43i3.2135","DOIUrl":"https://doi.org/10.51248/.v43i3.2135","url":null,"abstract":"Introduction and Aim: Anaemia is the most common deficiency disorder among people of all age groups, known as Paandu in the Siddha system of medicine. The test drug siddha poly mineral formulation Lavana Dravagam mentioned in Siddha literature kannukamiyam ennum vaithiya segaram, has been used for pandu (anaemia). Dravagam is a form of internal medicine, processed by distillation method and is also referred as Pugai neer, Shakthi neer, Dravaga neer. The study aimed to evaluate the haematinic activity and efficacy of poly mineral Siddha formulation Lavana Dravagam against phenylhydrazine induced anaemic albino Wistar rat model.\u0000 \u0000Materials and Methods: The animals were selected and divided into four groups (I, II, III, and IV) of six rats (n=6) each. Anaemia was induced by an oral administration of phenylhydrazine (single dose of 10 mg/kg per oral for 8 days). Group I served as normal control and Group II received standard drug hematinic syrup in suspension form at dose 2 mL/kg. Groups III, IV received the formulated oral indiffusible mixture of Lavana Dravagam at a dose of 0.02ml to 0.03ml/kg respectively. RBC, Hb, PCV, MCV, MCH, were analyzed as indices of anaemia. The mean corpuscular volume, mean corpuscular Hb, and mean corpuscular Hb concentration were calculated.\u0000 \u0000Results: From the literature evidence, acute toxicity evaluation and pharmacological studies, the drug Lavana Dravagam is found out to have hematinic activity. This study reveals that there is significant (P<0.05) increase in RBC count, Hb level, and PCV by administering phenylhydrazine within one week of treatment.\u0000 \u0000Conclusion: It could be concluded that the drug Lavana dravagam will have promising effects in the management of anaemia (Paandu).","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42181196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Adelia Ratnadita, Christnawati, Pinandi Sri Pudyani
Introduction and Aim: Exposure to blue light-emitting diode (blue-LED) during the stabilization period can prevent relapse by increasing the tension side osteoblast, which is characterized by increased levels of alkaline phosphatase (ALP) in the gingival crevicular fluid (GCF). Increasing ALP levels occurred from day 7 with the highest peak on day 14 post-stabilization. The purpose of this research was to analyze the effect of blue-LED exposure during the stabilization period on ALP levels in the tension side GCF of Wistar rats (Rattus norvegicus) at days 0, 3, 7, and 14 post-stabilizations. Materials and Methods: Ten male Wistar rats aged 2.5-3 months, weighing 200-250 grams were divided into two groups (control and LED group). An orthodontic force of 35 grams was applied to the mandibular inter incisors of Wistar rats using an open coil spring. Blue-LED (490 nm wavelength, 1000 mW/cm² light intensity) exposure for 30 seconds once a day during a 7-day stabilization period. Gingival crevicular fluid was taken with paper points on days 0, 3, 7, and 14 post-stabilizations for ALP levels measurement using an ELISA method. Two-way ANOVA and Post Hoc LSD statistical tests were performed. Results: The results showed LED group ALP levels were higher than the control group. There has been a significant increase in ALP levels on days 7 and 14 in the control and LED groups. Conclusion: It was concluded that blue-LED exposure increased tension side ALP levels. Increasing ALP levels occurred from day 7 with the highest peak level on day 14. Blue-LED exposure in the stabilization period is a favorable therapeutic option to accelerate alveolar bone formation due to being non-invasive, easy to apply clinically, and low cost.
{"title":"Blue-light emitting diode exposure effect on alkaline phosphatase levels post-orthodontic stabilization in rats","authors":"Adelia Ratnadita, Christnawati, Pinandi Sri Pudyani","doi":"10.51248/.v43i3.2080","DOIUrl":"https://doi.org/10.51248/.v43i3.2080","url":null,"abstract":"Introduction and Aim: Exposure to blue light-emitting diode (blue-LED) during the stabilization period can prevent relapse by increasing the tension side osteoblast, which is characterized by increased levels of alkaline phosphatase (ALP) in the gingival crevicular fluid (GCF). Increasing ALP levels occurred from day 7 with the highest peak on day 14 post-stabilization. The purpose of this research was to analyze the effect of blue-LED exposure during the stabilization period on ALP levels in the tension side GCF of Wistar rats (Rattus norvegicus) at days 0, 3, 7, and 14 post-stabilizations.\u0000 \u0000Materials and Methods: Ten male Wistar rats aged 2.5-3 months, weighing 200-250 grams were divided into two groups (control and LED group). An orthodontic force of 35 grams was applied to the mandibular inter incisors of Wistar rats using an open coil spring. Blue-LED (490 nm wavelength, 1000 mW/cm² light intensity) exposure for 30 seconds once a day during a 7-day stabilization period. Gingival crevicular fluid was taken with paper points on days 0, 3, 7, and 14 post-stabilizations for ALP levels measurement using an ELISA method. Two-way ANOVA and Post Hoc LSD statistical tests were performed.\u0000 \u0000Results: The results showed LED group ALP levels were higher than the control group. There has been a significant increase in ALP levels on days 7 and 14 in the control and LED groups.\u0000 \u0000Conclusion: It was concluded that blue-LED exposure increased tension side ALP levels. Increasing ALP levels occurred from day 7 with the highest peak level on day 14. Blue-LED exposure in the stabilization period is a favorable therapeutic option to accelerate alveolar bone formation due to being non-invasive, easy to apply clinically, and low cost.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42319194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rishika Dhimole, Mithilesh M. Dhamande, Seema R. Kambala, Prinyanka Paul Madhu
In dentistry precisely recording the soft tissue details and adjacent tooth morphology is a critical process. It is quite challenging for the dentist since the oral hard and soft tissue structures show great variations in every patient. Conventional impression materials such as agar, alginate and elastomeric impression materials are used but these impression materials require patient’s cooperation during impression taking. These impression materials sometimes cause gagging, irritation and unpleasant taste and smell to the patient. To overcome these drawbacks digital impression and digital scanners are encouraging innovations. They increase the patient’s comfort and aids in the accuracy in recording tissue details. The development of new technology in the medical and dental fields is leading to advancements that enable physicians to create treatments and materials that can enhance patients' quality of life. Different IOSs have adopted varied scanning methodologies, which could result in different scanning accuracies. This was done to compare the accuracy of several IOSs and the effects of different variables on the accuracy result. Using 3D scanning technologies, a physical model which is digital three-dimensional computer-aided design (CAD) is transformed. For the design and production of specific parts using additive manufacturing (AM) technologies, this digital output offers several advantages. It is crucial to evaluate the dentistry industry's present level of 3D scanning technology utilization.
{"title":"The digital era in dentistry: A review","authors":"Rishika Dhimole, Mithilesh M. Dhamande, Seema R. Kambala, Prinyanka Paul Madhu","doi":"10.51248/.v43i3.2865","DOIUrl":"https://doi.org/10.51248/.v43i3.2865","url":null,"abstract":"In dentistry precisely recording the soft tissue details and adjacent tooth morphology is a critical process. It is quite challenging for the dentist since the oral hard and soft tissue structures show great variations in every patient. Conventional impression materials such as agar, alginate and elastomeric impression materials are used but these impression materials require patient’s cooperation during impression taking. These impression materials sometimes cause gagging, irritation and unpleasant taste and smell to the patient. To overcome these drawbacks digital impression and digital scanners are encouraging innovations. They increase the patient’s comfort and aids in the accuracy in recording tissue details. The development of new technology in the medical and dental fields is leading to advancements that enable physicians to create treatments and materials that can enhance patients' quality of life. Different IOSs have adopted varied scanning methodologies, which could result in different scanning accuracies. This was done to compare the accuracy of several IOSs and the effects of different variables on the accuracy result. Using 3D scanning technologies, a physical model which is digital three-dimensional computer-aided design (CAD) is transformed. For the design and production of specific parts using additive manufacturing (AM) technologies, this digital output offers several advantages. It is crucial to evaluate the dentistry industry's present level of 3D scanning technology utilization.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48209505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sumana Maiti Das, A. Bose, Suparna Kanti Pal, Suman Meyur, Siddhartha Das, Aloke Ghosh Dastidar
Introduction and Aim: Radiation induced skin reactions (RISR) often delays the treatment affecting compliance and outcomes. Granulocyte colony stimulating factor (G-CSF) is known to recruit dermo-genic stem cells and help in healing. Very few studies are there to evaluate its potential in this regard. This study evaluates intradermal infiltration of G-CSF for its effectiveness, effect on treatment delay and quality of life in comparison to conventional treatment for grade 3/4 RISR. Materials and Methods: Patients experiencing advanced RISR due to any regimen of external beam radiation above 50 Gy of 2 Gy equivalent doses were randomized to undergo treatment either with intradermal G-CSF infiltration at margin and bases of lesion along with conventional therapies or with only conventional anti-inflammatory/ antibiotic therapy. The experimental arm and the control arm had 62 and 60 patients respectively analyzed for time taken for re-epithelization and treatment delay. Also.pre and two weeks post-treatment global quality of life and pain scores were taken for qualitative comparison between the arms. Results: G-CSF arm took a mean 5.2 (95percent CI=4.4-5.7) days, significantly less than conventional treatment arm (Mean=7.5 days, 95percent CI= 6.8-8.1,p=0.002) for re-epithelization. Grade of toxicity adjusted hazard ratio of non-resolution in 10 days time was 2.98 for the control arm over the experimental arm(p=.046). Treatment gap was significantly less in the G-CSF arm (48.4 percent vs 68.3percent, p=0.026), with average delay of treatment being significantly reduced (p 0.001). A ten-day delay adjusted survival was significantly better in the experimental arm (Mantel-Cox Log rank test, X2=12.26, p 0.001). Conclusion: Intradermal perilesional G-CSF infiltration for advanced RISR hastens wound healing compared to conventional treatment and significantly reduces the chances of treatment delay.
{"title":"A randomised phase II trial of intra-dermal G-CSF infiltration in radiation induced skin reactions","authors":"Sumana Maiti Das, A. Bose, Suparna Kanti Pal, Suman Meyur, Siddhartha Das, Aloke Ghosh Dastidar","doi":"10.51248/.v43i3.736","DOIUrl":"https://doi.org/10.51248/.v43i3.736","url":null,"abstract":"Introduction and Aim: Radiation induced skin reactions (RISR) often delays the treatment affecting compliance and outcomes. Granulocyte colony stimulating factor (G-CSF) is known to recruit dermo-genic stem cells and help in healing. Very few studies are there to evaluate its potential in this regard. This study evaluates intradermal infiltration of G-CSF for its effectiveness, effect on treatment delay and quality of life in comparison to conventional treatment for grade 3/4 RISR.\u0000 \u0000Materials and Methods: Patients experiencing advanced RISR due to any regimen of external beam radiation above 50 Gy of 2 Gy equivalent doses were randomized to undergo treatment either with intradermal G-CSF infiltration at margin and bases of lesion along with conventional therapies or with only conventional anti-inflammatory/ antibiotic therapy. The experimental arm and the control arm had 62 and 60 patients respectively analyzed for time taken for re-epithelization and treatment delay. Also.pre and two weeks post-treatment global quality of life and pain scores were taken for qualitative comparison between the arms.\u0000 \u0000Results: G-CSF arm took a mean 5.2 (95percent CI=4.4-5.7) days, significantly less than conventional treatment arm (Mean=7.5 days, 95percent CI= 6.8-8.1,p=0.002) for re-epithelization. Grade of toxicity adjusted hazard ratio of non-resolution in 10 days time was 2.98 for the control arm over the experimental arm(p=.046). Treatment gap was significantly less in the G-CSF arm (48.4 percent vs 68.3percent, p=0.026), with average delay of treatment being significantly reduced (p 0.001). A ten-day delay adjusted survival was significantly better in the experimental arm (Mantel-Cox Log rank test, X2=12.26, p 0.001).\u0000 \u0000Conclusion: Intradermal perilesional G-CSF infiltration for advanced RISR hastens wound healing compared to conventional treatment and significantly reduces the chances of treatment delay.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45123700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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