Pub Date : 2023-10-15DOI: 10.21931/rb/css/2023.08.03.4
Ali Mohammed Jaafer, Ali A. Abdul-Kareem
This study was conducted at the poultry field, College of Agriculture and the Marshes, University of Thi-Qar from 10/11/2021 to 26/12/2021, and the Molecular Genetics Laboratories at the Marsh Research Center to determine the FATP gene polymorphism on some productive and physiological traits of broilers of ROSS 308. A total of 150 birds were used. The results of this study showed the following. Three genotypes were identified in the sequence of nitrogenous bases in the presence of the mutation G237A: GG, GA, and AA. It was noted that there were significant differences in the distribution ratios of the genotypes of the FATP gene according to the mutation G237A, where the genotype GA recorded the highest percentage, followed by genotype GG and then genotype AA. The G allele frequency is superior to the A. It was noted that there were no significant differences for the genotypes of the G237A mutation on the body weight and no significant differences between the GA, GG and AA genotypes of the FATP gene on the body weight, a significant difference in the genotypes of the G237A mutation on carcass weight, AA genotype outperformed the GG and GA genotypes, the AA genotype was superior to the GA and GG genotypes on wings relative weight. Keywords: Polymorphism SLC27 gene, economic, carcass, broiler.
{"title":"Relationship between the SLC27 gene's polymorphism and some broiler carcass and economic factors","authors":"Ali Mohammed Jaafer, Ali A. Abdul-Kareem","doi":"10.21931/rb/css/2023.08.03.4","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.03.4","url":null,"abstract":"This study was conducted at the poultry field, College of Agriculture and the Marshes, University of Thi-Qar from 10/11/2021 to 26/12/2021, and the Molecular Genetics Laboratories at the Marsh Research Center to determine the FATP gene polymorphism on some productive and physiological traits of broilers of ROSS 308. A total of 150 birds were used. The results of this study showed the following. Three genotypes were identified in the sequence of nitrogenous bases in the presence of the mutation G237A: GG, GA, and AA. It was noted that there were significant differences in the distribution ratios of the genotypes of the FATP gene according to the mutation G237A, where the genotype GA recorded the highest percentage, followed by genotype GG and then genotype AA. The G allele frequency is superior to the A. It was noted that there were no significant differences for the genotypes of the G237A mutation on the body weight and no significant differences between the GA, GG and AA genotypes of the FATP gene on the body weight, a significant difference in the genotypes of the G237A mutation on carcass weight, AA genotype outperformed the GG and GA genotypes, the AA genotype was superior to the GA and GG genotypes on wings relative weight. Keywords: Polymorphism SLC27 gene, economic, carcass, broiler.","PeriodicalId":52287,"journal":{"name":"Revista Bionatura","volume":"77 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136183822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-15DOI: 10.21931/rb/css/2023.08.03.25
Ghaith A. A. Al-obaidy, Ali I. IlBas, Safae A. H. AL-kawthary
In this study, after a field survey of olive trees in some northern areas in the Kurdistan region of Iraq and the province of Dohuk, samples were collected for bacteria Pseudomonas Savastonifrom olive trees infected with olive knot—the period from the beginning of March until the end of May for the diagnosis of Pseudomonas Savastoni. After activating the bacteria, we started inoculating the bacteria on the fruits of other plants, such as carrots, lemons, beans, local apples and hidden apples. These fruits were subjected to the same environmental conditions of humidity, temperature and incubation period. However, no symptoms of infection of the fruits with this bacteria appeared. Only the carrots were found to be infected with this bacteria after 13 days of incubation, but after 20 days, the bacteria had infected all the carrots. We isolated the bacteria again from the infected carrot. We performed all microscopic, phenotypic and biochemical tests and subjected the bacterial isolates to a device determined using the VITEK2 system to identify the type of bacteria. Bacteria we conducted molecular tests on them, such as the test PCR and the test RT-PCR for the gene 16S r RNA. After the results appeared, the isolates were subjected to genetic sequencing to ensure the occurrence of genetic mutations of the gene 16S r RNA. Keywords: Pseudomonas Savastoni, 16S r RNA, RT-PCR, VITEK2, Sequencing
本研究对伊拉克库尔德斯坦北部和杜胡克省部分地区的橄榄树进行实地调查后，从感染橄榄结的橄榄树上采集了savaston假单胞菌的样本，时间为3月初至5月底，用于诊断savaston假单胞菌。在激活细菌后，我们开始将细菌接种到其他植物的果实上，如胡萝卜、柠檬、豆类、本地苹果和隐藏苹果。这些果实受到相同的湿度、温度和孵育期的环境条件。然而，果实没有出现这种细菌感染的症状。经过13天的孵育，只有胡萝卜被发现感染了这种细菌，但20天后，细菌感染了所有的胡萝卜。我们再次从被感染的胡萝卜中分离出细菌。我们进行了所有显微镜、表型和生化测试，并将细菌分离物置于使用VITEK2系统确定的设备中以鉴定细菌类型。我们对细菌进行了分子检测，如16S r RNA基因的PCR检测和RT-PCR检测。结果出现后，对分离株进行基因测序，确保16S r RNA基因发生突变。关键词:萨沃顿假单胞菌，16S r RNA, RT-PCR, VITEK2，测序
{"title":"In Vitro, Pathogenicity of Pseudomonas savasana Isolated from Olive Trees in Iraq on Fruits of Various Plant Species and its Detection by Polymerase Chain Reaction (BCR)","authors":"Ghaith A. A. Al-obaidy, Ali I. IlBas, Safae A. H. AL-kawthary","doi":"10.21931/rb/css/2023.08.03.25","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.03.25","url":null,"abstract":"In this study, after a field survey of olive trees in some northern areas in the Kurdistan region of Iraq and the province of Dohuk, samples were collected for bacteria Pseudomonas Savastonifrom olive trees infected with olive knot—the period from the beginning of March until the end of May for the diagnosis of Pseudomonas Savastoni. After activating the bacteria, we started inoculating the bacteria on the fruits of other plants, such as carrots, lemons, beans, local apples and hidden apples. These fruits were subjected to the same environmental conditions of humidity, temperature and incubation period. However, no symptoms of infection of the fruits with this bacteria appeared. Only the carrots were found to be infected with this bacteria after 13 days of incubation, but after 20 days, the bacteria had infected all the carrots. We isolated the bacteria again from the infected carrot. We performed all microscopic, phenotypic and biochemical tests and subjected the bacterial isolates to a device determined using the VITEK2 system to identify the type of bacteria. Bacteria we conducted molecular tests on them, such as the test PCR and the test RT-PCR for the gene 16S r RNA. After the results appeared, the isolates were subjected to genetic sequencing to ensure the occurrence of genetic mutations of the gene 16S r RNA. Keywords: Pseudomonas Savastoni, 16S r RNA, RT-PCR, VITEK2, Sequencing","PeriodicalId":52287,"journal":{"name":"Revista Bionatura","volume":"264 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136184012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-15DOI: 10.21931/rb/css/2023.08.04.56
Israa Yahia AL-Qadhi, Basima Qasim Hasan AL-Saadi
Interleukin 13 (IL-13) is an immune-regulatory cytokine, primarily secreted by activated T Helper-Type (Th) 2 cells, which inhibits inflammatory cytokine production in allergic asthma. Single nucleotide polymorphism SNP (1295685A˃G) of the IL-13 gene and its serum level was included in a case-control study on 60 Iraqi asthmatics and 60 controls. ELISSA-linked immune-sorbent assay was used to estimate blood serum levels. The real-time high-resolution melting polymerase-chain-reaction (Real-time PCR-HRM) method was used to determine this variant. The results showed that the mean level of IL-13 was high in asthmatic patients compared to the control group (22.90 pg/ml vs.13.99 pg/ml), respectively, and significant differences were (p=0.0001). ROC curve analysis of IL-13 described an AUC of 0.882. Regarding the allele and genotype of (A˃G 1295685) SNP, there were clear differences between patients and the control group, as the frequency of the GG +AG genotype was significantly higher (p=0.001) in patients compared to the control group (81.7%vs.23.3%) respectively, and the G allele frequency was higher in patients compared to the control group (48.8% vs. 16.6%) respectively, with significant difference (p=0.001). In conclusion, increased levels of IL-13 in serum and the presence of the G allele, GG + AG genotype in the IL13 gene may be associated with the pathogenesis of allergic asthma in Iraqi adult patients. Keywords: Allergic Asthma, IL-13, SNP, RT-HRM-PCR
{"title":"Polymorphism of IL13 (rs1295685) Gene and Its Serum Level in a Sample of Iraqi Patients with Allergic Asthma","authors":"Israa Yahia AL-Qadhi, Basima Qasim Hasan AL-Saadi","doi":"10.21931/rb/css/2023.08.04.56","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.04.56","url":null,"abstract":"Interleukin 13 (IL-13) is an immune-regulatory cytokine, primarily secreted by activated T Helper-Type (Th) 2 cells, which inhibits inflammatory cytokine production in allergic asthma. Single nucleotide polymorphism SNP (1295685A˃G) of the IL-13 gene and its serum level was included in a case-control study on 60 Iraqi asthmatics and 60 controls. ELISSA-linked immune-sorbent assay was used to estimate blood serum levels. The real-time high-resolution melting polymerase-chain-reaction (Real-time PCR-HRM) method was used to determine this variant. The results showed that the mean level of IL-13 was high in asthmatic patients compared to the control group (22.90 pg/ml vs.13.99 pg/ml), respectively, and significant differences were (p=0.0001). ROC curve analysis of IL-13 described an AUC of 0.882. Regarding the allele and genotype of (A˃G 1295685) SNP, there were clear differences between patients and the control group, as the frequency of the GG +AG genotype was significantly higher (p=0.001) in patients compared to the control group (81.7%vs.23.3%) respectively, and the G allele frequency was higher in patients compared to the control group (48.8% vs. 16.6%) respectively, with significant difference (p=0.001). In conclusion, increased levels of IL-13 in serum and the presence of the G allele, GG + AG genotype in the IL13 gene may be associated with the pathogenesis of allergic asthma in Iraqi adult patients. Keywords: Allergic Asthma, IL-13, SNP, RT-HRM-PCR","PeriodicalId":52287,"journal":{"name":"Revista Bionatura","volume":"124 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136184160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-15DOI: 10.21931/rb/css/2023.08.04.65
Hussein Wael Mahmood, Zakariya Bader Al Hamdani
The study was conducted in one of the fields of the Al-Yamja area, south of Mosul, which is (5) km from the city Centre for the season (2020-2021). Five genotypes of soft wheat were used, which were obtained from the Organization (C.I.M.M.Y.T.), adding an approved local cultivar (Abu Ghraib). The cultivation was carried out on lines with a length of (2) m and the distance between one line and another (30) cm using a randomized complete block design (R.C.B.D.) with three replications and in the manner explained by 1 .The study included the following traits: number of days to flowering at (50%), plant height (cm), spike length (cm), number of grains/spike, number of spikes/plant, vital yield (g/plant) and grain yield (g /plant), The weight of 100 grains and the harvest index %, where the analysis of variance table shows that all the genotypes showed significance at the probability level of 1% except for the trait number of spikes/plant, which was significant at the probability level of 5% and trait of the harvest index %. The statistical significance was not reached, and the studied phenotypic, genetic and environmental variance values showed that they were more significant than zero for all studied traits except for the genetic variance for the characteristic of harvest index %, Where it did not differ significantly from zero, it was noted that the values of heritability in the broad sense were high and for all the studied traits except for the trait number of spikes/plant was medium. The recipe of harvest index % was low. The values of the expected genetic improvement were as an average percentage for the traits of plant height, spike length and the number of grains/spike. The vital yield, grain yield and weight of 100 grains were low for the characteristics of the number of days to flowering at (50%), the number of spikes/plant and harvest index %. We note that the values of the genetic variation coefficient were the highest for a trait of spike length, number of grains/spike and biological yield, and the values of the phenotypic variation coefficient were the highest for the traits of spike length, number of grains/spike and biological yield. Keywords: genetic parameters, bread wheat, Triticum aestivum L
{"title":"Estimation of genetic parameters for new entries of bread wheat (Triticum aestivum L.)","authors":"Hussein Wael Mahmood, Zakariya Bader Al Hamdani","doi":"10.21931/rb/css/2023.08.04.65","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.04.65","url":null,"abstract":"The study was conducted in one of the fields of the Al-Yamja area, south of Mosul, which is (5) km from the city Centre for the season (2020-2021). Five genotypes of soft wheat were used, which were obtained from the Organization (C.I.M.M.Y.T.), adding an approved local cultivar (Abu Ghraib). The cultivation was carried out on lines with a length of (2) m and the distance between one line and another (30) cm using a randomized complete block design (R.C.B.D.) with three replications and in the manner explained by 1 .The study included the following traits: number of days to flowering at (50%), plant height (cm), spike length (cm), number of grains/spike, number of spikes/plant, vital yield (g/plant) and grain yield (g /plant), The weight of 100 grains and the harvest index %, where the analysis of variance table shows that all the genotypes showed significance at the probability level of 1% except for the trait number of spikes/plant, which was significant at the probability level of 5% and trait of the harvest index %. The statistical significance was not reached, and the studied phenotypic, genetic and environmental variance values showed that they were more significant than zero for all studied traits except for the genetic variance for the characteristic of harvest index %, Where it did not differ significantly from zero, it was noted that the values of heritability in the broad sense were high and for all the studied traits except for the trait number of spikes/plant was medium. The recipe of harvest index % was low. The values of the expected genetic improvement were as an average percentage for the traits of plant height, spike length and the number of grains/spike. The vital yield, grain yield and weight of 100 grains were low for the characteristics of the number of days to flowering at (50%), the number of spikes/plant and harvest index %. We note that the values of the genetic variation coefficient were the highest for a trait of spike length, number of grains/spike and biological yield, and the values of the phenotypic variation coefficient were the highest for the traits of spike length, number of grains/spike and biological yield. Keywords: genetic parameters, bread wheat, Triticum aestivum L","PeriodicalId":52287,"journal":{"name":"Revista Bionatura","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136184167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-15DOI: 10.21931/rb/css/2023.08.04.68
Mustafa sami saeed Al obaidi, Hisham Ahmed Saleh Al-mashhadani
The experiment was conducted in the poultry field of the Department of Animal Production - College of Agricultural Engineering Sciences - University of Baghdad / Abu Ghraib from 20/9/20/2021 to 10/31/2021 (42 days). To study the effect of adding a blend of herbal powders (Turmeric powder, anise, coriander, cumin, Cinnamomum) to broiler diets on some physiological traits and some indicators of oxidation in meat. In the field experiment, 240 broiler chicks, 308 Ross, obtained from Al-Shukr Broiler Production Company, located in Baghdad / Abu Ghraib province, with an average initial weight of 40.28 g, were used. Each treatment included four replicates of 10 chicks/duplicate, and the experimental treatments were as follows: T1 control (free of adding herbs), and T2 control treatment containing antibiotic (oxytetracycline 0.05%) and T5, T4, T3 and T a mixture of herbs in proportions (0.25, 0.50, 0.75, 1%)As this mixture includes five types of aromatic plants (Turmeric powder, anise, coriander, cumin, Cinnamomum), The results showed that there were no significant differences in the percentage of dressing, the treatment T4 (0.50%) achieved the highest weight of the chest and neck piece. In contrast, T5 and T6 achieved a significant decrease in the weight of the neck. There were no significant differences in the relative weight of the edible internal viscera, abdominal fat, and Vibricia gland; as for the physiological traits, the two treatments, T5 and T6, achieved a significant decrease in the urea concentration, and there was no significant difference between the treatments in creatine. There was a significant decrease in cholesterol concentration for all additional treatments. In contrast, for oxidation indicators, the T5 treatment achieved a decrease in the value of peroxide, with a significant increase in the value of glutathione before storage and 30 days after storage. Keywords: herbal powders, turmeric, cumin, anise, Cinnamon, coriander, broiler diets, oxidation of meat
{"title":"Effect of adding a combination of herbal powders (turmeric, cumin, anise, Cinnamon and coriander) in broiler diets on the percentage of dressing, qualitative traits of the carcass, physiological traits and some indicators of oxidation of meat","authors":"Mustafa sami saeed Al obaidi, Hisham Ahmed Saleh Al-mashhadani","doi":"10.21931/rb/css/2023.08.04.68","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.04.68","url":null,"abstract":"The experiment was conducted in the poultry field of the Department of Animal Production - College of Agricultural Engineering Sciences - University of Baghdad / Abu Ghraib from 20/9/20/2021 to 10/31/2021 (42 days). To study the effect of adding a blend of herbal powders (Turmeric powder, anise, coriander, cumin, Cinnamomum) to broiler diets on some physiological traits and some indicators of oxidation in meat. In the field experiment, 240 broiler chicks, 308 Ross, obtained from Al-Shukr Broiler Production Company, located in Baghdad / Abu Ghraib province, with an average initial weight of 40.28 g, were used. Each treatment included four replicates of 10 chicks/duplicate, and the experimental treatments were as follows: T1 control (free of adding herbs), and T2 control treatment containing antibiotic (oxytetracycline 0.05%) and T5, T4, T3 and T a mixture of herbs in proportions (0.25, 0.50, 0.75, 1%)As this mixture includes five types of aromatic plants (Turmeric powder, anise, coriander, cumin, Cinnamomum), The results showed that there were no significant differences in the percentage of dressing, the treatment T4 (0.50%) achieved the highest weight of the chest and neck piece. In contrast, T5 and T6 achieved a significant decrease in the weight of the neck. There were no significant differences in the relative weight of the edible internal viscera, abdominal fat, and Vibricia gland; as for the physiological traits, the two treatments, T5 and T6, achieved a significant decrease in the urea concentration, and there was no significant difference between the treatments in creatine. There was a significant decrease in cholesterol concentration for all additional treatments. In contrast, for oxidation indicators, the T5 treatment achieved a decrease in the value of peroxide, with a significant increase in the value of glutathione before storage and 30 days after storage. Keywords: herbal powders, turmeric, cumin, anise, Cinnamon, coriander, broiler diets, oxidation of meat","PeriodicalId":52287,"journal":{"name":"Revista Bionatura","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136184168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-15DOI: 10.21931/rb/css/2023.08.04.83
Asalah Mojaz Tawfeeq Ghafar, Shaymaa Naji Dahham
The study included 147 oral swabs collected from dental caries patients between October 2020 and January 2021. The culture results on the isolation medium showed that 73 samples, 49.7% of the total samples, showed positive growth, while 74 samples or 50.3%, showed no bacterial growth. p . gingivalis isolates produced hemolysin and biofilm formation by 100%. Their ability to produce B-lactamase was decreased, and the percentage of isolates produced was 42.5%, and the percentage of protease-producing isolates was 20.5%. As for the enzyme lipase and urease, all isolates could not produce them. The results showed that all bacterial isolates were resistant to all the antibiotics used, namely Piperacillin - Ciprofloxacin - Levofloxacin - Carbenicillin - oxytetracycline - Azithromycin - Meropene - Norfloxacin at 0%, 0%, 60.3%, 100%, 0%, 60.3%, 60.3%, 9.6%, respectively. Extracts of cloves, pomegranate peel and miswak (aqueous and alcoholic) were used at four concentrations of 75, 100, 150 and 200 mg/ml, and they showed different inhibitory activities against the studied bacteria depending on the concentration. 200 mg/ml is among the most effective inhibitors, especially alcoholic extracts. For these plants, the alcoholic extracts of pomegranate peel, cloves and toothpicks recorded the highest inhibitory activity, with 52, 48 and 25 mm diameters, respectively. The lowest inhibitory activity of the alcohol extract was a concentration of 75 mg, and the highest inhibitory activity was observed against pomegranate peels with a diameter of 25 mm cloves and toothpicks with a diameter of 22 and 8 mm, respectively. Keywords: Porphyromonas gingivalis, Multidrug resistance, inhibition activity, Plant extraction, virulence factors
{"title":"Determination of the inhibitory activity of some plant extracts against the obligate anaerobic bacteria Porphyromonas gingivalis that causes dental caries.","authors":"Asalah Mojaz Tawfeeq Ghafar, Shaymaa Naji Dahham","doi":"10.21931/rb/css/2023.08.04.83","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.04.83","url":null,"abstract":"The study included 147 oral swabs collected from dental caries patients between October 2020 and January 2021. The culture results on the isolation medium showed that 73 samples, 49.7% of the total samples, showed positive growth, while 74 samples or 50.3%, showed no bacterial growth. p . gingivalis isolates produced hemolysin and biofilm formation by 100%. Their ability to produce B-lactamase was decreased, and the percentage of isolates produced was 42.5%, and the percentage of protease-producing isolates was 20.5%. As for the enzyme lipase and urease, all isolates could not produce them. The results showed that all bacterial isolates were resistant to all the antibiotics used, namely Piperacillin - Ciprofloxacin - Levofloxacin - Carbenicillin - oxytetracycline - Azithromycin - Meropene - Norfloxacin at 0%, 0%, 60.3%, 100%, 0%, 60.3%, 60.3%, 9.6%, respectively. Extracts of cloves, pomegranate peel and miswak (aqueous and alcoholic) were used at four concentrations of 75, 100, 150 and 200 mg/ml, and they showed different inhibitory activities against the studied bacteria depending on the concentration. 200 mg/ml is among the most effective inhibitors, especially alcoholic extracts. For these plants, the alcoholic extracts of pomegranate peel, cloves and toothpicks recorded the highest inhibitory activity, with 52, 48 and 25 mm diameters, respectively. The lowest inhibitory activity of the alcohol extract was a concentration of 75 mg, and the highest inhibitory activity was observed against pomegranate peels with a diameter of 25 mm cloves and toothpicks with a diameter of 22 and 8 mm, respectively. Keywords: Porphyromonas gingivalis, Multidrug resistance, inhibition activity, Plant extraction, virulence factors","PeriodicalId":52287,"journal":{"name":"Revista Bionatura","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136184325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-15DOI: 10.21931/rb/css/2023.08.04.91
Talib M. Talib, Taghreed Fadhil Zaidan
The nutritional status of patients with inflammatory bowel disease is considered less than ideal. This study aimed to compare the salivary levels of some trace elements (Zinc, copper, and magnesium) in patients with ulcerative colitis (UC) on two different therapeutic modalities to control subjects. Materials and methods: This cross-sectional study was performed in 2021-2022 on 53 diagnosed UC patients (27 UC patients on oral immunosuppressant therapy and 26 UC patients on combination therapy). All subjects were randomly selected from gastroenterology and Hepatology teaching hospitals in Baghdad city; twenty-five subjects were also recruited as controls. Demographic data were recorded through a checklist. Salivary levels of zinc (Zn), copper (Cu) and magnesium (Mg) were measured by atomic absorption spectroscopy. Data were analyzed using SPSS-28 at a significance level of 0.05. The results: Salivary Zn mean was significantly lower in monotherapy and combination therapy patients than in controls (p<0.001). Mean salivary Cu levels were significantly higher in monotherapy and combination therapy patients than in controls (p<0.001). Similarly, the salivary Mg mean was significantly lower in both groups of ulcerative colitis patients than in controls (p<0.001). The mean of salivary Cu was higher in UC patients, while the men of Zn and Mg were lower in UC patients compared to controls. Conclusions: The results showed that UC patients have significantly lower Zn and Mg levels than healthy individuals and were lower inpatient on monotherapy than in combination. At the same time, salivary Cu in UC patients was higher than in control individuals and was higher in UC patients on monotherapy. Keywords: Inflammatory bowel diseases, Ulcerative colitis, Trace elements
{"title":"Salivary trace elements in patients with ulcerative colitis on different treatment modalities","authors":"Talib M. Talib, Taghreed Fadhil Zaidan","doi":"10.21931/rb/css/2023.08.04.91","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.04.91","url":null,"abstract":"The nutritional status of patients with inflammatory bowel disease is considered less than ideal. This study aimed to compare the salivary levels of some trace elements (Zinc, copper, and magnesium) in patients with ulcerative colitis (UC) on two different therapeutic modalities to control subjects. Materials and methods: This cross-sectional study was performed in 2021-2022 on 53 diagnosed UC patients (27 UC patients on oral immunosuppressant therapy and 26 UC patients on combination therapy). All subjects were randomly selected from gastroenterology and Hepatology teaching hospitals in Baghdad city; twenty-five subjects were also recruited as controls. Demographic data were recorded through a checklist. Salivary levels of zinc (Zn), copper (Cu) and magnesium (Mg) were measured by atomic absorption spectroscopy. Data were analyzed using SPSS-28 at a significance level of 0.05. The results: Salivary Zn mean was significantly lower in monotherapy and combination therapy patients than in controls (p<0.001). Mean salivary Cu levels were significantly higher in monotherapy and combination therapy patients than in controls (p<0.001). Similarly, the salivary Mg mean was significantly lower in both groups of ulcerative colitis patients than in controls (p<0.001). The mean of salivary Cu was higher in UC patients, while the men of Zn and Mg were lower in UC patients compared to controls. Conclusions: The results showed that UC patients have significantly lower Zn and Mg levels than healthy individuals and were lower inpatient on monotherapy than in combination. At the same time, salivary Cu in UC patients was higher than in control individuals and was higher in UC patients on monotherapy. Keywords: Inflammatory bowel diseases, Ulcerative colitis, Trace elements","PeriodicalId":52287,"journal":{"name":"Revista Bionatura","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136184329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-15DOI: 10.21931/rb/css/2023.08.03.61
Mohsen Kamel Mohammad Ali, Jalal Hameed Hamza, Ahmed Shihab Ahmed
A laboratory experiment was carried out according to a completely randomized design with four repetitions on the seeds resulting from a field experiment applied for the two seasons, 2020 and 2021, to find out the effect of the cultivars (Ambar 33, Yasamin, Dijlah, Ambar Al-Baraka and Furat 1) and the harvest dates (at physiological maturity and after 7 and 14, 21 and 28 days of physiological maturity) on the vigour of rice seeds. The results showed the superiority of the seeds of the cultivar Anbar Al-Baraka at first and final counting, dry weight of the seedling, seedling vigor index and electrical conductivity, and the superiority of the seeds of Dijla cultivar at accelerated aging test and cold test without significant difference with the Anbar Al-Baraka cultivar in both seasons. The harvest date exceeded 14 days after physiological maturity at all studied traits in both seasons. Anbar Al-Baraka at the harvest 14 days after physiological maturity was superior at most studied traits. It can be concluded that the seeds of the cultivar Amber Al-Baraka showed the best performance at seed vigor, primarily harvested after 14 days of physiological maturity. Keywords: accelerating aging, cold test, Oryza sativa, first count, seedling vigour, physiological maturity
{"title":"Effect of cultivars and harvest dates on rice seeds vigore","authors":"Mohsen Kamel Mohammad Ali, Jalal Hameed Hamza, Ahmed Shihab Ahmed","doi":"10.21931/rb/css/2023.08.03.61","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.03.61","url":null,"abstract":"A laboratory experiment was carried out according to a completely randomized design with four repetitions on the seeds resulting from a field experiment applied for the two seasons, 2020 and 2021, to find out the effect of the cultivars (Ambar 33, Yasamin, Dijlah, Ambar Al-Baraka and Furat 1) and the harvest dates (at physiological maturity and after 7 and 14, 21 and 28 days of physiological maturity) on the vigour of rice seeds. The results showed the superiority of the seeds of the cultivar Anbar Al-Baraka at first and final counting, dry weight of the seedling, seedling vigor index and electrical conductivity, and the superiority of the seeds of Dijla cultivar at accelerated aging test and cold test without significant difference with the Anbar Al-Baraka cultivar in both seasons. The harvest date exceeded 14 days after physiological maturity at all studied traits in both seasons. Anbar Al-Baraka at the harvest 14 days after physiological maturity was superior at most studied traits. It can be concluded that the seeds of the cultivar Amber Al-Baraka showed the best performance at seed vigor, primarily harvested after 14 days of physiological maturity. Keywords: accelerating aging, cold test, Oryza sativa, first count, seedling vigour, physiological maturity","PeriodicalId":52287,"journal":{"name":"Revista Bionatura","volume":"234 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135760120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-15DOI: 10.21931/rb/css/2023.08.03.59
R. A. A. Al-asadi, Sajed A. Mohamed
Agricultural Engineering Sciences - University of Baghdad for the fall season 2016. Aloe vera seedlings were selected with a high degree of homogeneity in terms of vegetative growth and number of leaves, and the plants were distributed as a factorial experiment according to the RCBD randomized complete block design, with three replications and an average of six plants in the experimental unit. Results revealed significant differences in most of the studied traits. They showed the superiority of the N3P3 interference treatment by giving the highest values in the number of leaves, leaf width and thickness, fresh weight of leaves and plant yield of dry matter (15.83 leaves. and 217.46 g. plant-1), respectively. As for the effect of spraying with amino acids, treatment N3 excelled in the characteristics of leaf thickness, fresh weight of leaves and plant yield of dry matter, which amounted to 1.828 mm. leaf and 273.0 g. leaf and 157.23 g. leaf-1 in sequence. The treatment of P3 spraying surpassed the characteristic of leaf thickness and plant yield of dry matter, which amounted to 1.822 mm. leaf-1 and 191.61 g. plant-1. The results did not show significant differences between spraying with amino acids and phosphorous treatments in plant length and leaf width. Keywords: Aloe vera, biostimulant, amino acids, phosphorous
农业工程科学-巴格达大学2016年秋季。选取营养生长和叶片数量均高度均匀的芦荟幼苗，按RCBD随机完全区组设计进行因子试验，3个重复，平均6株为试验单位。结果显示，所研究的大多数性状存在显著差异。在叶片数、叶片宽度和厚度、叶片鲜重和干物质单株产量(15.83片)方面，N3P3干预处理的优势显著。和217.46 g. plant-1)。在氨基酸喷施效果方面，N3处理在叶片厚度、叶片鲜重和干物质单株产量方面表现较好，分别为1.828 mm /叶、273.0 g /叶和157.23 g /叶。P3喷施处理超过了干物质叶厚和单株产量特征，分别达到1.822 mm. leaf-1和191.61 g. plant-1。结果表明，施氨基酸与施磷处理在植株长度和叶宽方面无显著差异。关键词:芦荟，生物刺激素，氨基酸，磷
{"title":"Response of Aleo vera plant to different concentrations of phosphorous and terra-sorb complex and its effect on vegetative growth characteristics and its gel content","authors":"R. A. A. Al-asadi, Sajed A. Mohamed","doi":"10.21931/rb/css/2023.08.03.59","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.03.59","url":null,"abstract":"Agricultural Engineering Sciences - University of Baghdad for the fall season 2016. Aloe vera seedlings were selected with a high degree of homogeneity in terms of vegetative growth and number of leaves, and the plants were distributed as a factorial experiment according to the RCBD randomized complete block design, with three replications and an average of six plants in the experimental unit. Results revealed significant differences in most of the studied traits. They showed the superiority of the N3P3 interference treatment by giving the highest values in the number of leaves, leaf width and thickness, fresh weight of leaves and plant yield of dry matter (15.83 leaves. and 217.46 g. plant-1), respectively. As for the effect of spraying with amino acids, treatment N3 excelled in the characteristics of leaf thickness, fresh weight of leaves and plant yield of dry matter, which amounted to 1.828 mm. leaf and 273.0 g. leaf and 157.23 g. leaf-1 in sequence. The treatment of P3 spraying surpassed the characteristic of leaf thickness and plant yield of dry matter, which amounted to 1.822 mm. leaf-1 and 191.61 g. plant-1. The results did not show significant differences between spraying with amino acids and phosphorous treatments in plant length and leaf width. Keywords: Aloe vera, biostimulant, amino acids, phosphorous","PeriodicalId":52287,"journal":{"name":"Revista Bionatura","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135760389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-15DOI: 10.21931/rb/css/2023.08.04.72
Kurdistan A. AlSalam A. AlKarem, Suzan .Kamran. Hasan
Bacteriocins were produced from Lactobacillus bulgaricus, Streptococcus thermophiles and MIX (Lactobacillus bulgaricus, Streptococcus thermophiles) by two methods: Shaker Fermentation and Static Fermentation (aerobic and anaerobic) through bacterial growth in MRS liquid medium, for each of them and the detection of the inhibitory activity on some types of pathogenic bacteria through the growth of the test bacteria on a solid nutrient medium Agar Nutrient. It was found that the three isolates can produce bacteriocin, and there are significant differences between each of the bacteriocins towards the pathogenic bacteria. It shows that the bacteriocin produced from Lactobacillus bulgaricus has a higher inhibitory activity and can inhibit different types of bacteria from gram-positive and gram-negative keywords: Doxazosin, Chloroquine, Synergism, MCF-7, Autophagy. Keywords: Shaker Fermentation. Static Fermentation. Bacteriocins. Lactic acid bacteria.
{"title":"The inhibition of selected pathogens by Lactobacillus bulgaricus and Streptococcus thermophiles bacteriocins.","authors":"Kurdistan A. AlSalam A. AlKarem, Suzan .Kamran. Hasan","doi":"10.21931/rb/css/2023.08.04.72","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.04.72","url":null,"abstract":"Bacteriocins were produced from Lactobacillus bulgaricus, Streptococcus thermophiles and MIX (Lactobacillus bulgaricus, Streptococcus thermophiles) by two methods: Shaker Fermentation and Static Fermentation (aerobic and anaerobic) through bacterial growth in MRS liquid medium, for each of them and the detection of the inhibitory activity on some types of pathogenic bacteria through the growth of the test bacteria on a solid nutrient medium Agar Nutrient. It was found that the three isolates can produce bacteriocin, and there are significant differences between each of the bacteriocins towards the pathogenic bacteria. It shows that the bacteriocin produced from Lactobacillus bulgaricus has a higher inhibitory activity and can inhibit different types of bacteria from gram-positive and gram-negative keywords: Doxazosin, Chloroquine, Synergism, MCF-7, Autophagy. Keywords: Shaker Fermentation. Static Fermentation. Bacteriocins. Lactic acid bacteria.","PeriodicalId":52287,"journal":{"name":"Revista Bionatura","volume":"45 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135760924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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