Pub Date : 2023-07-01DOI: 10.20873/jbb.uft.cemaf.v11n2.azevedo
Vitória Virgínia Soares Azevedo, Leandro Alves de Souza, Virlane Kelly Lima Hunaldo, Leonardo Hunaldo dos Santos, Gabrielli Nunes Climaco, Marcos Silva de Sousa, Leticia Nunes dos Santos
O inhame é um tubérculo nativo de regiões tropicais, rico em nutrientes como proteínas, fibras, fósforo, potássio e vitaminas do complexo B, objetivou neste trabalho produzir chips de inhame e realizar a caracterização microbiológica, físico-química e sensorial dos produtos, os mesmos sendo submetidos a secagem artificial e a fritura em óleo de girassol. Os chips de inhame foram submetidos ao pré-tratamento osmótico com concentração de 18% de sal, 6% de cebola desidratada, 6% de açafrão, 6% de alho em pó e 6% de orégano por 5h a 55°C. Os chips foram avaliados quanto às características físico-químicas de atividade de água, 0,56 (formulação 1); 0,40(formulação 2); pH, 6,38 e 5,90, formulação 1 e 2, respectivamente e coloração considerando as coordenadas L*, a* e b*. Na avaliação sensorial foi utilizada a estrutura de escala hedônica de 9 pontos e o teste de intenção de compra. A formulação 2 foi mais aceita entre todos os atributos sensoriais, cor, aroma, sabor, textura, aparência e impressão global.
{"title":"Processamento e caracterização de chips de inhame saborizados","authors":"Vitória Virgínia Soares Azevedo, Leandro Alves de Souza, Virlane Kelly Lima Hunaldo, Leonardo Hunaldo dos Santos, Gabrielli Nunes Climaco, Marcos Silva de Sousa, Leticia Nunes dos Santos","doi":"10.20873/jbb.uft.cemaf.v11n2.azevedo","DOIUrl":"https://doi.org/10.20873/jbb.uft.cemaf.v11n2.azevedo","url":null,"abstract":"O inhame é um tubérculo nativo de regiões tropicais, rico em nutrientes como proteínas, fibras, fósforo, potássio e vitaminas do complexo B, objetivou neste trabalho produzir chips de inhame e realizar a caracterização microbiológica, físico-química e sensorial dos produtos, os mesmos sendo submetidos a secagem artificial e a fritura em óleo de girassol. Os chips de inhame foram submetidos ao pré-tratamento osmótico com concentração de 18% de sal, 6% de cebola desidratada, 6% de açafrão, 6% de alho em pó e 6% de orégano por 5h a 55°C. Os chips foram avaliados quanto às características físico-químicas de atividade de água, 0,56 (formulação 1); 0,40(formulação 2); pH, 6,38 e 5,90, formulação 1 e 2, respectivamente e coloração considerando as coordenadas L*, a* e b*. Na avaliação sensorial foi utilizada a estrutura de escala hedônica de 9 pontos e o teste de intenção de compra. A formulação 2 foi mais aceita entre todos os atributos sensoriais, cor, aroma, sabor, textura, aparência e impressão global.","PeriodicalId":52402,"journal":{"name":"Journal of Tropical Biodiversity and Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86918177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-01DOI: 10.20873/jbb.uft.cemaf.v11n2.belfort
Cristóvam Colombo Belfort, Daniel Aleff Dantas Martins, Gabriela Almeida de Paula, Agenor Francisco Rocha Júnior, Raimundo Tomaz Da Costa Filho
A Cebolinha (Allium schoenoprasum) é uma planta condimentar, aromática, folhosa, apreciada em todo o Brasil, com importante papel social, preponderante nos projetos de agricultura familiar. Muito embora sendo uma espécie com elevada cerosidade foliar, propriedade que lhe confere uma certa tolerância à desidratação, colocadas no conservador doméstico, em poucos dias sofre descoloração, comprometendo a qualidade do produto, redundando em consideráveis perdas, sobretudo, para o consumidor. Diante disso, o presente trabalho buscou avaliar a conservação pós-colheita de folhas da cebolinha, submetidas ao acondicionamento em baixa temperatura. Todo o processamento ocorreu no laboratório do Núcleo de Estudos, Pesquisas e Processamento de Alimentos (NUEPPA) do Centro de Ciências Agrárias (CCA) da Universidade Federal do Piauí, em Teresina, entre agosto e novembro de 2020. Os tratamentos consistiram das análises de amostras retiradas quinzenalmente até o limite de 75 dias, ficando o tratamento testemunha correspondendo às amostras, recém-colhidas, as quais também foram submetidas às determinações de natureza físico-química. Verificações complementares de natureza sensorial foram realizadas, envolvendo cor, aroma e textura, sendo então considerados atributos de qualidade. A refrigeração favoreceu o prolongamento da vida útil da cebolinha, sem variação nas características físico-químicas testadas, demonstrando que é possível conservá-la em refrigerador à baixa temperatura (-20oC) acondicionada em sacos plásticos por, pelo menos 75 dias, sem comprometimento da qualidade do produto; otimizando o aproveitamento da mão de obra familiar, criando as perspectivas de lançamento de um novo produto no rol dos congelados.
{"title":"Conservação pós-colheita da cebolinha em baixa temperatura como sinôni-mo de otimização produtiva","authors":"Cristóvam Colombo Belfort, Daniel Aleff Dantas Martins, Gabriela Almeida de Paula, Agenor Francisco Rocha Júnior, Raimundo Tomaz Da Costa Filho","doi":"10.20873/jbb.uft.cemaf.v11n2.belfort","DOIUrl":"https://doi.org/10.20873/jbb.uft.cemaf.v11n2.belfort","url":null,"abstract":"A Cebolinha (Allium schoenoprasum) é uma planta condimentar, aromática, folhosa, apreciada em todo o Brasil, com importante papel social, preponderante nos projetos de agricultura familiar. Muito embora sendo uma espécie com elevada cerosidade foliar, propriedade que lhe confere uma certa tolerância à desidratação, colocadas no conservador doméstico, em poucos dias sofre descoloração, comprometendo a qualidade do produto, redundando em consideráveis perdas, sobretudo, para o consumidor. Diante disso, o presente trabalho buscou avaliar a conservação pós-colheita de folhas da cebolinha, submetidas ao acondicionamento em baixa temperatura. Todo o processamento ocorreu no laboratório do Núcleo de Estudos, Pesquisas e Processamento de Alimentos (NUEPPA) do Centro de Ciências Agrárias (CCA) da Universidade Federal do Piauí, em Teresina, entre agosto e novembro de 2020. Os tratamentos consistiram das análises de amostras retiradas quinzenalmente até o limite de 75 dias, ficando o tratamento testemunha correspondendo às amostras, recém-colhidas, as quais também foram submetidas às determinações de natureza físico-química. Verificações complementares de natureza sensorial foram realizadas, envolvendo cor, aroma e textura, sendo então considerados atributos de qualidade. A refrigeração favoreceu o prolongamento da vida útil da cebolinha, sem variação nas características físico-químicas testadas, demonstrando que é possível conservá-la em refrigerador à baixa temperatura (-20oC) acondicionada em sacos plásticos por, pelo menos 75 dias, sem comprometimento da qualidade do produto; otimizando o aproveitamento da mão de obra familiar, criando as perspectivas de lançamento de um novo produto no rol dos congelados.","PeriodicalId":52402,"journal":{"name":"Journal of Tropical Biodiversity and Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89215535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-01DOI: 10.20873/jbb.uft.cemaf.v11n2.luz
Petterson Baptista Da Luz, Kathia Fernandes Lopes Pivetta
As palmeiras são exploradas e cultivadas para os mais diversos fins, entre eles o ornamental. A propagação das palmeiras é feita, quase que exclusivamente, por sementes, porém, há grande variação no processo de germinação, influenciada por diversos fatores, temperatura do ambiente e período entre a colheita e o armazenamento. A espécie Ptychosperma macarthurii é uma espécie de valor ornamental e tem sido objeto de estudos sobre o processo de formação de mudas. Portanto, este trabalho teve como objetivo estudar o efeito do armazenamento temporário na germinação de sementes. O delineamento experimental utilizado foi inteiramente casualizado. Para o estudo de armazenamento foram utilizados 7 tratamentos (semeadura logo após a colheita, 1, 2, 3, 4, 5 e 6 semanas após); com 4 repetições de 25 diásporos. Os diásporos foram colocados em caixa de tipo gerbox, contendo vermiculita que ficaram em câmara de germinação a 25°C, já as sementes armazenadas, permaneceram em condições de laboratório. Foram calculados a porcentagem de germinação e o Índice de Velocidade de Germinação e realizada a análise de regressão polinomial. Concluiu-se que a porcentagem de germinação aumentou com o armazenamento atingindo seu maior ponto após 3 semanas de armazenamento, a velocidade de germinação aumentou durante o armazenamento até a quinta semana.
{"title":"Efeito do armazenamento temporário na germinação de sementes de Ptychosperma macarthurii","authors":"Petterson Baptista Da Luz, Kathia Fernandes Lopes Pivetta","doi":"10.20873/jbb.uft.cemaf.v11n2.luz","DOIUrl":"https://doi.org/10.20873/jbb.uft.cemaf.v11n2.luz","url":null,"abstract":"As palmeiras são exploradas e cultivadas para os mais diversos fins, entre eles o ornamental. A propagação das palmeiras é feita, quase que exclusivamente, por sementes, porém, há grande variação no processo de germinação, influenciada por diversos fatores, temperatura do ambiente e período entre a colheita e o armazenamento. A espécie Ptychosperma macarthurii é uma espécie de valor ornamental e tem sido objeto de estudos sobre o processo de formação de mudas. Portanto, este trabalho teve como objetivo estudar o efeito do armazenamento temporário na germinação de sementes. O delineamento experimental utilizado foi inteiramente casualizado. Para o estudo de armazenamento foram utilizados 7 tratamentos (semeadura logo após a colheita, 1, 2, 3, 4, 5 e 6 semanas após); com 4 repetições de 25 diásporos. Os diásporos foram colocados em caixa de tipo gerbox, contendo vermiculita que ficaram em câmara de germinação a 25°C, já as sementes armazenadas, permaneceram em condições de laboratório. Foram calculados a porcentagem de germinação e o Índice de Velocidade de Germinação e realizada a análise de regressão polinomial. Concluiu-se que a porcentagem de germinação aumentou com o armazenamento atingindo seu maior ponto após 3 semanas de armazenamento, a velocidade de germinação aumentou durante o armazenamento até a quinta semana.","PeriodicalId":52402,"journal":{"name":"Journal of Tropical Biodiversity and Biotechnology","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135454659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The date of the paleoclimate event was ascertained using a biostratigraphic analysis. The thickness of the Oyo Formation was measured using measurements with a Jacob stick method, yielding a thickness of 80.8 meters and 23 rock samples. In the Southern Mountains Zone, new result of the age of the upper part of the Oyo Formation by biostratigraphy investigation of the hill Late Miocene (9.79 Ma to 5.78 Ma). Biostratigraphic investigation in the Oyo River revealed 28 species and 9 genera, with two datums. The study area was classified into three biozonations based on the datum found Globorotalia acostaensis/M13a/N16 zone, the lower Globorotalia plesiotumida/M13b/N17 zone, and the upper Globigerinoides conglobatus/M14/N17 zone. The results of a paleoclimate analysis on the Oyo River Section show a general cooling tendency in the study area. Seven paleoclimate zones can be determined from these trends consisting of four warm and three cold zones. Zone I (warm), zone II (cold), zone III (warm), and zone IV (cold) have the coldest peak in the study region in 8.3 Ma, zone V (warm), and zone VI (cold), followed by zone VII (warm). Based on the correlation with other studies (South China Sea, Pacific Ocean, Oyo River, Ngalang River, and Ngioro Section), paleoclimate events in the study area occur globally.
{"title":"Biostratigraphy and Climate Change in the Late Miocene Age Based on Foraminifera in the Oyo Formation, Oyo River Section, Gunung Kidul, Yogyakarta","authors":"Adesti Audina Ulfah, A. Akmaluddin, D. Barianto","doi":"10.22146/jtbb.81769","DOIUrl":"https://doi.org/10.22146/jtbb.81769","url":null,"abstract":"The date of the paleoclimate event was ascertained using a biostratigraphic analysis. The thickness of the Oyo Formation was measured using measurements with a Jacob stick method, yielding a thickness of 80.8 meters and 23 rock samples. In the Southern Mountains Zone, new result of the age of the upper part of the Oyo Formation by biostratigraphy investigation of the hill Late Miocene (9.79 Ma to 5.78 Ma). Biostratigraphic investigation in the Oyo River revealed 28 species and 9 genera, with two datums. The study area was classified into three biozonations based on the datum found Globorotalia acostaensis/M13a/N16 zone, the lower Globorotalia plesiotumida/M13b/N17 zone, and the upper Globigerinoides conglobatus/M14/N17 zone. The results of a paleoclimate analysis on the Oyo River Section show a general cooling tendency in the study area. Seven paleoclimate zones can be determined from these trends consisting of four warm and three cold zones. Zone I (warm), zone II (cold), zone III (warm), and zone IV (cold) have the coldest peak in the study region in 8.3 Ma, zone V (warm), and zone VI (cold), followed by zone VII (warm). Based on the correlation with other studies (South China Sea, Pacific Ocean, Oyo River, Ngalang River, and Ngioro Section), paleoclimate events in the study area occur globally. ","PeriodicalId":52402,"journal":{"name":"Journal of Tropical Biodiversity and Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43759487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Muthi’ Ikawati, Hajidah Musyayyadah, Y. Putri, Ummi Maryam Zulfin, F. Wulandari, Dyaningtyas Dewi Pamungkas Putri, E. Meiyanto
Pentagamavunone-1 (PGV-1) is a curcumin analog with a prominent anti-cancer potency in vitro and in vivo for several cancer types, including colon cancer. Combining PGV-1 with natural compounds such as diosmin, galangin, and piperine can enhance its effectiveness due to their promising chemoprevention properties. We aimed to evaluate the effectiveness of combining PGV-1 with diosmin, galangin, or piperine for colon cancer by using in vitro and bioinformatic approaches to predict their target proteins. WiDr cells were used as a model for colon adenocarcinoma (COAD). The cell viability under a single or combination treatment of PGV-1 and diosmin, galangin, or piperine was evaluated using direct counting by the trypan blue exclusion test. SwissTargetProtein, UALCAN, and OncoLnc were utilized to predict target proteins of the compounds in COAD, the expression level of target proteins in COAD, and the survival rate of patients with overexpressed target proteins, respectively. The IC50 values for PGV-1, diosmin, galangin, and piperine were 2.8´10-2 µg/mL, 81 µg/mL, 7 µg/mL, and 172 µg/mL, respectively. All the tested natural compounds showed synergistic effects when combined with PGV-1 at low concentrations. Eleven proteins that were overexpressed in COAD were identified as potential targets. Overlapped predicted targets of PGV-1 and galangin or piperine were CDK1, MET, and TOP2A. The high expression of another set of predicted target proteins, SCD, CA9, and SQLE, led to lower survival rates in COAD patients. We concluded that combinations of PGV-1 with natural compounds can synergistically enhane its anti-cancer activity for colon cancer.
{"title":"The Synergistic Effect of Combination of Pentagamavunone-1 with Diosmin, Galangin, and Piperine in WiDr Colon Cancer Cells: In vitro and Target Protein Prediction","authors":"Muthi’ Ikawati, Hajidah Musyayyadah, Y. Putri, Ummi Maryam Zulfin, F. Wulandari, Dyaningtyas Dewi Pamungkas Putri, E. Meiyanto","doi":"10.22146/jtbb.80975","DOIUrl":"https://doi.org/10.22146/jtbb.80975","url":null,"abstract":"Pentagamavunone-1 (PGV-1) is a curcumin analog with a prominent anti-cancer potency in vitro and in vivo for several cancer types, including colon cancer. Combining PGV-1 with natural compounds such as diosmin, galangin, and piperine can enhance its effectiveness due to their promising chemoprevention properties. We aimed to evaluate the effectiveness of combining PGV-1 with diosmin, galangin, or piperine for colon cancer by using in vitro and bioinformatic approaches to predict their target proteins. WiDr cells were used as a model for colon adenocarcinoma (COAD). The cell viability under a single or combination treatment of PGV-1 and diosmin, galangin, or piperine was evaluated using direct counting by the trypan blue exclusion test. SwissTargetProtein, UALCAN, and OncoLnc were utilized to predict target proteins of the compounds in COAD, the expression level of target proteins in COAD, and the survival rate of patients with overexpressed target proteins, respectively. The IC50 values for PGV-1, diosmin, galangin, and piperine were 2.8´10-2 µg/mL, 81 µg/mL, 7 µg/mL, and 172 µg/mL, respectively. All the tested natural compounds showed synergistic effects when combined with PGV-1 at low concentrations. Eleven proteins that were overexpressed in COAD were identified as potential targets. Overlapped predicted targets of PGV-1 and galangin or piperine were CDK1, MET, and TOP2A. The high expression of another set of predicted target proteins, SCD, CA9, and SQLE, led to lower survival rates in COAD patients. We concluded that combinations of PGV-1 with natural compounds can synergistically enhane its anti-cancer activity for colon cancer.","PeriodicalId":52402,"journal":{"name":"Journal of Tropical Biodiversity and Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47165255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. El-Hakim, S. Sunarti, L. Hidayati, S. Widiyanto
Metabolic syndrome is associated with abnormalities of lipid levels in the blood such as hyperlipidemia. Hyperlipidemia conditions can increase the risk of atherosclerosis and hypertension. Beetroot (Beta vulgaris L.) is a plant that contains high antioxidants. Beetroot has the potency to be used as a functional food that can reduce the potential for atherosclerosis and blood pressure. The aim of this study was to examine the effect of beetroot-enriched feed on the cholesterol level, atherogenic index and blood pressure of rats (Rattus norvegicus Berkenhout, 1769) induced by high fat and fructose diet. As many as 25 rats were divided into control, hyperlipidemia, and three treatment groups. The hyperlipidemia and treatment groups were induced to become hyperlipidemia using AIN93-M modified high fat and fructose feed for 8 weeks. The treatment groups were followed by intervention with 6, 9, and 12% beetroot enriched feed for 6 weeks. The feed was prepared by mixing beetroot flour in the pellets. Blood pressure, total cholesterol, and HDL level measurement was conducted after hyperlipidemia induction and after the intervention. The data analyzed with one-way ANOVA, DMRT, and T-Test. The results showed that 6% beetroot intervention have the highest increasing of HDL-cholesterol than other groups. The 9% beetroot intervention significantly decrease total cholesterol lower than normal baseline, and 12% beetroot intervention significantly decrease blood pressure than other groups. The atherogenic index of all treatment group was decreased. The 9% beetroot enriched feed was seen as an optimum dose to reduce total cholesterol, atherogenic index and blood pressure and increase HDL-cholesterol.
{"title":"Anti-hypercholesterolemia, Anti-atherogenic, and Anti-hypertension Effects of Red Beetroot (Beta vulgaris L.) in Rats Induced by High Fat and Fructose Diet","authors":"A. El-Hakim, S. Sunarti, L. Hidayati, S. Widiyanto","doi":"10.22146/jtbb.75914","DOIUrl":"https://doi.org/10.22146/jtbb.75914","url":null,"abstract":"Metabolic syndrome is associated with abnormalities of lipid levels in the blood such as hyperlipidemia. Hyperlipidemia conditions can increase the risk of atherosclerosis and hypertension. Beetroot (Beta vulgaris L.) is a plant that contains high antioxidants. Beetroot has the potency to be used as a functional food that can reduce the potential for atherosclerosis and blood pressure. The aim of this study was to examine the effect of beetroot-enriched feed on the cholesterol level, atherogenic index and blood pressure of rats (Rattus norvegicus Berkenhout, 1769) induced by high fat and fructose diet. As many as 25 rats were divided into control, hyperlipidemia, and three treatment groups. The hyperlipidemia and treatment groups were induced to become hyperlipidemia using AIN93-M modified high fat and fructose feed for 8 weeks. The treatment groups were followed by intervention with 6, 9, and 12% beetroot enriched feed for 6 weeks. The feed was prepared by mixing beetroot flour in the pellets. Blood pressure, total cholesterol, and HDL level measurement was conducted after hyperlipidemia induction and after the intervention. The data analyzed with one-way ANOVA, DMRT, and T-Test. The results showed that 6% beetroot intervention have the highest increasing of HDL-cholesterol than other groups. The 9% beetroot intervention significantly decrease total cholesterol lower than normal baseline, and 12% beetroot intervention significantly decrease blood pressure than other groups. The atherogenic index of all treatment group was decreased. The 9% beetroot enriched feed was seen as an optimum dose to reduce total cholesterol, atherogenic index and blood pressure and increase HDL-cholesterol. ","PeriodicalId":52402,"journal":{"name":"Journal of Tropical Biodiversity and Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41399179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. S. D. Irsyam, Muhammad Rifqi Hariri, A. P. Dewi, R. R. Irwanto
The presence of naturalized Alocasia cucullata (Lour.) G.Don (Araceae) in Java is reported for the first time in this paper. The species is an introduced ornamental plant native to India, Sri Lanka, and Indo-China and was only known in cultivation. In this study we collected samples from its naturalized populations in Sukabumi Regency (Cibadak Subdistrict), Sumedang Regency (Jatinangor and Tanjungsari Subdistrict). The observed population grows along the roadside, coastal, ITB Jatinangor green space area, and palm oil plantation. In nature, A. cucullata may spread vegetatively either through root suckers, corms, and stem fragments. The description, distribution map, photographs, and a brief discussion are provided here.
{"title":"First Report on The Naturalized Alocasia cucullata (Araceae) in Java, Indonesia","authors":"A. S. D. Irsyam, Muhammad Rifqi Hariri, A. P. Dewi, R. R. Irwanto","doi":"10.22146/jtbb.73456","DOIUrl":"https://doi.org/10.22146/jtbb.73456","url":null,"abstract":"The presence of naturalized Alocasia cucullata (Lour.) G.Don (Araceae) in Java is reported for the first time in this paper. The species is an introduced ornamental plant native to India, Sri Lanka, and Indo-China and was only known in cultivation. In this study we collected samples from its naturalized populations in Sukabumi Regency (Cibadak Subdistrict), Sumedang Regency (Jatinangor and Tanjungsari Subdistrict). The observed population grows along the roadside, coastal, ITB Jatinangor green space area, and palm oil plantation. In nature, A. cucullata may spread vegetatively either through root suckers, corms, and stem fragments. The description, distribution map, photographs, and a brief discussion are provided here. ","PeriodicalId":52402,"journal":{"name":"Journal of Tropical Biodiversity and Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43504157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nur Indah Septriani, M. Rusydan, Gizela Aulia Agustin, Nareta Defiani, Fajar Sofyantoro, A. Hananya, D. Setyono
The current study observed the histological differences of the swim bladder of the tropical eel, Anguilla bicolor bicolor, as an adaptation resulting from hydrostatic change. A total of 15 eels were collected from Pasir Puncu, Keburuhan, Purworejo and Segara Anakan, Cilacap, Indonesia in June 2017, September 2020, and April 2021. The eels were grouped into 4 stages based on the silvering stage and sex, namely: yellow undifferentiated, yellow female, silver male, and silver female. The average length and body weight of yellow undifferentiated eels were 255.07 ± 45.91 mm and 13.66 ± 8.5 g, respectively; for yellow female, the values were 374.35 ± 41.51 mm and 56.5 ± 12.02 g; for silver male, the values were 432.43 ± 15.15 mm and 140.29 ± 13.85 g; and for silver female were 702 ± 0.00 mm and 545 ± 11.31 g. The present study successfully recorded the histological structure of the swim bladder of A. bicolor bicolor in silver male and silver female stages. Silver males and females displayed a greater significant development of the swim bladder than yellow stages in the gas gland, mucosa, and submucosa layers. These results suggest that an increase in the gas gland thickness allows a greater contribution from gas to gas secretion, the mucosa exerts a mechanical effect on the newly formed gas bubbles, and the submucosa thickness reduces gas conductivity from the swim bladder wall.
本研究观察了热带鳗鲡(Anguilla bicolor bicolor)的鱼鳔在水静力变化下的组织学差异。2017年6月、2020年9月和2021年4月，在印度尼西亚奇拉卡普的Pasir punu、Keburuhan、Purworejo和Segara Anakan共采集了15只鳗鱼。根据发银期和性别将黄鳝分为未分化黄鳝、黄雌黄鳝、银雄黄鳝和银雌黄鳝4个阶段。黄鳝平均体长为255.07±45.91 mm，平均体重为13.66±8.5 g;黄雌鼠为374.35±41.51 mm、56.5±12.02 g;银色雄性为432.43±15.15 mm, 140.29±13.85 g;银发女性为702±0.00 mm, 545±11.31 g。本研究成功地记录了银雄、银雌两期双色银鱼鱼鳔的组织学结构。与黄色阶段相比，银色雄性和雌性鱼鳔在气腺、粘膜和粘膜下层的发育更为显著。这些结果表明，气腺厚度的增加使得气体对气体的分泌有更大的贡献，粘膜对新形成的气泡产生机械作用，粘膜下层厚度的增加降低了来自鳔壁的气体传导。
{"title":"Differences in Swim Bladder Histology of Anguilla bicolor bicolor at Various Stages of Sexual Maturity","authors":"Nur Indah Septriani, M. Rusydan, Gizela Aulia Agustin, Nareta Defiani, Fajar Sofyantoro, A. Hananya, D. Setyono","doi":"10.22146/jtbb.74702","DOIUrl":"https://doi.org/10.22146/jtbb.74702","url":null,"abstract":"The current study observed the histological differences of the swim bladder of the tropical eel, Anguilla bicolor bicolor, as an adaptation resulting from hydrostatic change. A total of 15 eels were collected from Pasir Puncu, Keburuhan, Purworejo and Segara Anakan, Cilacap, Indonesia in June 2017, September 2020, and April 2021. The eels were grouped into 4 stages based on the silvering stage and sex, namely: yellow undifferentiated, yellow female, silver male, and silver female. The average length and body weight of yellow undifferentiated eels were 255.07 ± 45.91 mm and 13.66 ± 8.5 g, respectively; for yellow female, the values were 374.35 ± 41.51 mm and 56.5 ± 12.02 g; for silver male, the values were 432.43 ± 15.15 mm and 140.29 ± 13.85 g; and for silver female were 702 ± 0.00 mm and 545 ± 11.31 g. The present study successfully recorded the histological structure of the swim bladder of A. bicolor bicolor in silver male and silver female stages. Silver males and females displayed a greater significant development of the swim bladder than yellow stages in the gas gland, mucosa, and submucosa layers. These results suggest that an increase in the gas gland thickness allows a greater contribution from gas to gas secretion, the mucosa exerts a mechanical effect on the newly formed gas bubbles, and the submucosa thickness reduces gas conductivity from the swim bladder wall. ","PeriodicalId":52402,"journal":{"name":"Journal of Tropical Biodiversity and Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48018808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The qPCR method requires an oligonucleotide pair to prime the amplification process. With the variety of qPCR reagent and primer options available, in silico and laboratory experimental validation approach was needed to validate the most suitable primer for prior use. This article aims to provide in silico analysis of actin alpha-2 smooth muscle (Acta2), fibroblast activation protein (Fap), hypoxanthine phosphoribosyltransferase-1 (Hprt1), platelet-derived growth factor subunit B (Pdgfb), phosphoinositide-3-kinase regulatory subunit-1 (Pik3r1), and vascular cell adhesion molecule-1 (Vcam1) qPCR primer with qPCR and electrophoresis validation. The procedure used in this approach was in silico analysis of primer from published articles and newly designed primer. The analysis was done with Primer-BLAST for gene specificity, Primer-Dimer, OligoCalc for hairpin formation, BLAST Nucleotide for identical sequence screening, and Clustal Omega for product length validation. Experimental validation was done using qPCR for optimal annealing temperature, priming ability, and amplificon specificity, and electrophoresis for product length validation. This assessment resulted in in silico and laboratory experimental validation of Acta2, Fap, Hprt1, Pdgfb, Pik3r1, and Vcam1 primer pairs producing suitable amplicon for qPCR using Rattus norvegicus cDNA with SYBR annealing temperature range of 60-65°C with three mM MgCl2. The primer pair can be used for further qPCR analysis under similar conditions and the procedure stated can be used as starting point for qPCR Primer preparation.
{"title":"In Silico and Validation Approaches for Optimum Conditions of Rattus norvegicus Target Gene qPCR Primers","authors":"G. A. Pollo, N. Antara, F. Alamsyah, R. Pratiwi","doi":"10.22146/jtbb.71765","DOIUrl":"https://doi.org/10.22146/jtbb.71765","url":null,"abstract":"The qPCR method requires an oligonucleotide pair to prime the amplification process. With the variety of qPCR reagent and primer options available, in silico and laboratory experimental validation approach was needed to validate the most suitable primer for prior use. This article aims to provide in silico analysis of actin alpha-2 smooth muscle (Acta2), fibroblast activation protein (Fap), hypoxanthine phosphoribosyltransferase-1 (Hprt1), platelet-derived growth factor subunit B (Pdgfb), phosphoinositide-3-kinase regulatory subunit-1 (Pik3r1), and vascular cell adhesion molecule-1 (Vcam1) qPCR primer with qPCR and electrophoresis validation. The procedure used in this approach was in silico analysis of primer from published articles and newly designed primer. The analysis was done with Primer-BLAST for gene specificity, Primer-Dimer, OligoCalc for hairpin formation, BLAST Nucleotide for identical sequence screening, and Clustal Omega for product length validation. Experimental validation was done using qPCR for optimal annealing temperature, priming ability, and amplificon specificity, and electrophoresis for product length validation. This assessment resulted in in silico and laboratory experimental validation of Acta2, Fap, Hprt1, Pdgfb, Pik3r1, and Vcam1 primer pairs producing suitable amplicon for qPCR using Rattus norvegicus cDNA with SYBR annealing temperature range of 60-65°C with three mM MgCl2. The primer pair can be used for further qPCR analysis under similar conditions and the procedure stated can be used as starting point for qPCR Primer preparation.","PeriodicalId":52402,"journal":{"name":"Journal of Tropical Biodiversity and Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42952380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Indonesian leaffish Pristolepis grooti, an endemic species, are distributed in the region of Sumatra, Riau, Bangka Belitung and Kalimantan. However, there has been a decline in the population recently. This research purposed to investigate the mitochondrial DNA cytochrome c oxidase subunit I (COI) gene, the genetic distance, the genetic tree of the leaffish and characterize the chemical physics of water of its habitat in the Kelekar River Muara Enim Regency and the Ogan River, Ogan Ilir Regency. The method used in species authentication was DNA isolation, amplification using PCR (Polymerase Chain Reaction) and sequencing of COI gene. The size of the COI mtDNA gene fragment was 704 bp (PM 1, PM 4, PP 2 and PP 4) and 723 bp (PM 2, PM 3, PP 1 and PP 3). A cryptic diversity of the species P. grooti is found based on the genetic distance value of 4.5-6%, both in the Kelakar and Ogan Rivers. The phylogenetic tree of the leaffish of this study formed 2 separate sub-clusters with a bootstrap value of 50%. The properties of water qualities in the two rivers included temperatures 28.3-31.8°C, pH 5.6-8.3, dissolved oxygen 4.82-10.89 mg L-1, alkalinity 10-28 mg L-1 CaCO3, water transparency 16-45 cm, ammonia 0.47-0.70 mg L-1, water current 0.17-0.30 m s-1 and TDS 7-44 mg L-1.
印尼叶鱼Pristolepis grooti是一种特有物种，分布于苏门答腊、廖内岛、邦加-贝利东和加里曼丹地区。然而，最近人口有所下降。本研究旨在研究叶鱼线粒体DNA细胞色素c氧化酶亚基I（COI）基因、遗传距离、遗传树，并对其在Kelekar River Muara Enim Regency和Ogan River Ogan Ilir Regency栖息地的水化学物理特性进行表征。物种鉴定采用的方法是DNA分离、聚合酶链式反应扩增和COI基因测序。COI mtDNA基因片段的大小分别为704bp（PM 1、PM 4、PP 2和PP 4）和723bp（PM 2、PM 3、PP 1和PP 3）。在克拉卡尔河和奥甘河，根据4.5-6%的遗传距离值，发现了grooti物种的隐蔽多样性。本研究的叶鱼系统发育树形成了2个独立的亚簇，bootstrap值为50%。两条河流的水质特性包括温度28.3-31.8°C，pH 5.6-8.3，溶解氧4.82-10.89 mg L-1，碱度10-28 mg L-1 CaCO3，水透明度16-45 cm，氨0.47-0.70 mg L-1、水流0.17-0.30 m s-1和TDS 7-44 mg L-1。
{"title":"DNA Authentication of Indonesian Leaffish Pristolepis grooti from Kelekar River and Ogan River in South Sumatra Based on Cytochrome C Oxidase Subunit I (COI) Gene","authors":"M. Syaifudin","doi":"10.22146/jtbb.74133","DOIUrl":"https://doi.org/10.22146/jtbb.74133","url":null,"abstract":"Indonesian leaffish Pristolepis grooti, an endemic species, are distributed in the region of Sumatra, Riau, Bangka Belitung and Kalimantan. However, there has been a decline in the population recently. This research purposed to investigate the mitochondrial DNA cytochrome c oxidase subunit I (COI) gene, the genetic distance, the genetic tree of the leaffish and characterize the chemical physics of water of its habitat in the Kelekar River Muara Enim Regency and the Ogan River, Ogan Ilir Regency. The method used in species authentication was DNA isolation, amplification using PCR (Polymerase Chain Reaction) and sequencing of COI gene. The size of the COI mtDNA gene fragment was 704 bp (PM 1, PM 4, PP 2 and PP 4) and 723 bp (PM 2, PM 3, PP 1 and PP 3). A cryptic diversity of the species P. grooti is found based on the genetic distance value of 4.5-6%, both in the Kelakar and Ogan Rivers. The phylogenetic tree of the leaffish of this study formed 2 separate sub-clusters with a bootstrap value of 50%. The properties of water qualities in the two rivers included temperatures 28.3-31.8°C, pH 5.6-8.3, dissolved oxygen 4.82-10.89 mg L-1, alkalinity 10-28 mg L-1 CaCO3, water transparency 16-45 cm, ammonia 0.47-0.70 mg L-1, water current 0.17-0.30 m s-1 and TDS 7-44 mg L-1. ","PeriodicalId":52402,"journal":{"name":"Journal of Tropical Biodiversity and Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48334121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：