Rina Dwi Agustiani, O. Oedjijono, N. Rahmani, N. Ekowati
The objectives of the study were to isolate amylolytic bacteria from the rhizosphere and plant tissue of Canna edulis Ker., as well as litter; to know oligosaccharide compounds produced from starch hydrolyzed by the bacterial enzymes, and to identify the amylolytic bacteria based on phenetic and 16S rRNA gene sequences. From the rhizosphere, Canna plant tissue, and litters obtained thirty-two amylolytic bacterial isolates. Eight isolates (TH6, TH7, T5, T10, D2, D3, A3, S1) produced high clear zone diameters ranging from 18-30 mm; especially an isolate T10, which was consistent in producing a total clear zone diameter of 20 mm. The hydrolysate of starch hydrolysed by the T10 amylase resulted in three oligosaccharide compounds maltotriose, maltotetraose, and maltopentose. The amylase activity of isolate T10 was optimal at a temperature of 40°C and pH at 0.801 U/mL. The isolate T10 was identified as a species member of Bacillus toyonensis based on phenotyphic characterization and 16S rDNA gene sequencing analysis with a similarity value of 99.93%
{"title":"Isolation and Characterization of Rhizospheric Bacteria Associated with Canna Plant for Production of Maltooligosaccharide Amylase","authors":"Rina Dwi Agustiani, O. Oedjijono, N. Rahmani, N. Ekowati","doi":"10.22146/jtbb.78346","DOIUrl":"https://doi.org/10.22146/jtbb.78346","url":null,"abstract":"The objectives of the study were to isolate amylolytic bacteria from the rhizosphere and plant tissue of Canna edulis Ker., as well as litter; to know oligosaccharide compounds produced from starch hydrolyzed by the bacterial enzymes, and to identify the amylolytic bacteria based on phenetic and 16S rRNA gene sequences. From the rhizosphere, Canna plant tissue, and litters obtained thirty-two amylolytic bacterial isolates. Eight isolates (TH6, TH7, T5, T10, D2, D3, A3, S1) produced high clear zone diameters ranging from 18-30 mm; especially an isolate T10, which was consistent in producing a total clear zone diameter of 20 mm. The hydrolysate of starch hydrolysed by the T10 amylase resulted in three oligosaccharide compounds maltotriose, maltotetraose, and maltopentose. The amylase activity of isolate T10 was optimal at a temperature of 40°C and pH at 0.801 U/mL. The isolate T10 was identified as a species member of Bacillus toyonensis based on phenotyphic characterization and 16S rDNA gene sequencing analysis with a similarity value of 99.93%","PeriodicalId":52402,"journal":{"name":"Journal of Tropical Biodiversity and Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44533643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hurria Hurria, A. Alfian, Muhammad Fajri Ramadhan Muslim Saleh, H. Djamaludin, Murni Mursyid, Witno Witno, A. S. Mahulette
Etlingera acanthodes A.D. Poulsen is a ginger endemic to Sulawesi, and there is no research on its essential oils. The Essential oil information of E. acanthodes is the first reported and has a high novelty. The objective of the study is to analyze the component of E. acanthodes essential oils, which are endemic to Sulawesi. The essential oils obtained by hydrodistillation of the leaves, stems, flowers, and rhizomes of E. acanthodes A.D. Poulsen. The samples were analyzed by GC-MS method using the Agilent Technologies 7890 Gas Chromatograph with Auto Sampler. Data analysis of essential oils of GC-MS results were determined based on comparing mass spectra from the NIST 2005 v.2.0 library and Wiley 7 library 2003. The oils of E. acanthodes contain terpenoids, phenolic, steroids, and other compounds. Phenolic compounds (39.56%) predominate in leaves, other compounds (41.25%) on stems, diterpenes hydrocarbons (77.3%) in flowers, and other compounds (43.5%) and steroids (40.4%) on rhizomes. The five main compounds of E. acanthodes are Neophytadiene; (+)-De-O-Methylcentrolobine; Cholest-5-En-3-Ol,23-Ethyl-,(3. Beta.23S)-; 9,12-Octadecadienoic Acid, Phenol, 2-ethyl-. The analysis found several compounds that can be used for industry and medicine in the future.
{"title":"Essential Oils of Etlingera acanthodes A.D. Poulsen, An Endemic Ginger from Sulawesi Island","authors":"Hurria Hurria, A. Alfian, Muhammad Fajri Ramadhan Muslim Saleh, H. Djamaludin, Murni Mursyid, Witno Witno, A. S. Mahulette","doi":"10.22146/jtbb.72117","DOIUrl":"https://doi.org/10.22146/jtbb.72117","url":null,"abstract":"Etlingera acanthodes A.D. Poulsen is a ginger endemic to Sulawesi, and there is no research on its essential oils. The Essential oil information of E. acanthodes is the first reported and has a high novelty. The objective of the study is to analyze the component of E. acanthodes essential oils, which are endemic to Sulawesi. The essential oils obtained by hydrodistillation of the leaves, stems, flowers, and rhizomes of E. acanthodes A.D. Poulsen. The samples were analyzed by GC-MS method using the Agilent Technologies 7890 Gas Chromatograph with Auto Sampler. Data analysis of essential oils of GC-MS results were determined based on comparing mass spectra from the NIST 2005 v.2.0 library and Wiley 7 library 2003. The oils of E. acanthodes contain terpenoids, phenolic, steroids, and other compounds. Phenolic compounds (39.56%) predominate in leaves, other compounds (41.25%) on stems, diterpenes hydrocarbons (77.3%) in flowers, and other compounds (43.5%) and steroids (40.4%) on rhizomes. The five main compounds of E. acanthodes are Neophytadiene; (+)-De-O-Methylcentrolobine; Cholest-5-En-3-Ol,23-Ethyl-,(3. Beta.23S)-; 9,12-Octadecadienoic Acid, Phenol, 2-ethyl-. The analysis found several compounds that can be used for industry and medicine in the future. ","PeriodicalId":52402,"journal":{"name":"Journal of Tropical Biodiversity and Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42274134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dini Wahyu Kartika Sari, Himawan Achmad, Hafit Rahman, Harya Bimasuci
Flowerhorn fish has been known as a breed of fish produced by artificial hybridization between several cichlid fish. Other ornamental cichlid fish generally known to be crossed to make flowerhorn variant includes Amphilophus citrinelus, Amphilophus labiatus, Vieja melanurus, and Amphilophus trimaculatus. Our study identified a variety of flowerhorn samples with distinct morphotypes, dubbed as Cencu (LH1CC), Kamfa (LH2KF), Thai Silk (LH3TS), Kirin (LH4KR), Parrot (LH5PR), and Vieja (LH6VJ) using mtDNA COI-based DNA barcoding. Molecular analysis and phylogenetics showed that all sample had 0% genetic divergence and conspecific with A. trimaculatus sequence. Hence, we concluded that despite having varied morphotypes, all flowerhorn samples were identified as A. trimaculatus and were a variation of flowerhorn from A. trimaculatus lineage. The findings should be used as a precaution as the fish is identified as an invasive species.
{"title":"Molecular Identification of Several Morphologically Distinct Flowerhorn Fish (Family) Using Mitochondrial COI Gene Marker","authors":"Dini Wahyu Kartika Sari, Himawan Achmad, Hafit Rahman, Harya Bimasuci","doi":"10.22146/jtbb.78459","DOIUrl":"https://doi.org/10.22146/jtbb.78459","url":null,"abstract":"Flowerhorn fish has been known as a breed of fish produced by artificial hybridization between several cichlid fish. Other ornamental cichlid fish generally known to be crossed to make flowerhorn variant includes Amphilophus citrinelus, Amphilophus labiatus, Vieja melanurus, and Amphilophus trimaculatus. Our study identified a variety of flowerhorn samples with distinct morphotypes, dubbed as Cencu (LH1CC), Kamfa (LH2KF), Thai Silk (LH3TS), Kirin (LH4KR), Parrot (LH5PR), and Vieja (LH6VJ) using mtDNA COI-based DNA barcoding. Molecular analysis and phylogenetics showed that all sample had 0% genetic divergence and conspecific with A. trimaculatus sequence. Hence, we concluded that despite having varied morphotypes, all flowerhorn samples were identified as A. trimaculatus and were a variation of flowerhorn from A. trimaculatus lineage. The findings should be used as a precaution as the fish is identified as an invasive species. ","PeriodicalId":52402,"journal":{"name":"Journal of Tropical Biodiversity and Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45516365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. P. Sari, A. Persada, W. Mustaqim, K. Putri, Imti Yazil Wafa
Langsa Urban Forest (LUF) is a 10-ha of the isolated urban forest in Langsa, Aceh, which is maintained to preserve urban biodiversity such as the butterfly. No recent study has been done in this area on butterfly biodiversity including the diversity and plant's potential for host and food plant sources. A one-month survey in July 2021 using the standard walk method on four transects was conducted. There are 36 species recorded during this study including 5 families, with Nymphalidae as the most abundant family and Leptosia nina as the most abundant species. Shannon-Wiener diversity index was used for this recent research with the value of H’ 1.78–2.78 and the Evenness index with scale 0.66–0.94. Most of the species have broad geographical ranges, with 3 of them restricted to the Indomalayan realm. There are 117 plants were recorded which 33 species supposed as host and food plants divided into 26 as host plants, 11 as food plants, and 4 for both. No specific plants threatened the collected butterflies, but it's important since providing diversity data.
{"title":"Butterfly Diversity from Isolated Lowland Area: An Assessment in Langsa Urban Forest, Langsa, Aceh, Indonesia","authors":"H. P. Sari, A. Persada, W. Mustaqim, K. Putri, Imti Yazil Wafa","doi":"10.22146/jtbb.74610","DOIUrl":"https://doi.org/10.22146/jtbb.74610","url":null,"abstract":"Langsa Urban Forest (LUF) is a 10-ha of the isolated urban forest in Langsa, Aceh, which is maintained to preserve urban biodiversity such as the butterfly. No recent study has been done in this area on butterfly biodiversity including the diversity and plant's potential for host and food plant sources. A one-month survey in July 2021 using the standard walk method on four transects was conducted. There are 36 species recorded during this study including 5 families, with Nymphalidae as the most abundant family and Leptosia nina as the most abundant species. Shannon-Wiener diversity index was used for this recent research with the value of H’ 1.78–2.78 and the Evenness index with scale 0.66–0.94. Most of the species have broad geographical ranges, with 3 of them restricted to the Indomalayan realm. There are 117 plants were recorded which 33 species supposed as host and food plants divided into 26 as host plants, 11 as food plants, and 4 for both. No specific plants threatened the collected butterflies, but it's important since providing diversity data. ","PeriodicalId":52402,"journal":{"name":"Journal of Tropical Biodiversity and Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47818954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bantimurung Bulusaraung National Park is an area that has the largest karst ecosystem in Indonesia. Karst is prone to damage and difficult to reuse, so it requires conservation efforts. One of the plant species that can maintain this sustainability is Ficus which acts as key species in karst ecosystems. However, at this time the species is experiencing disturbances, one of which is due to the experience of invasive plants that can threaten the existence of Ficus because the weeds can colonize habitats and are dominant which can change species diversity. This study aimed to analyze the diversity and distribution of Ficus, compare species composition, and analyze environmental factors that affect Ficus spp. in Pattunuang Resort and Bantimurung Resort. Vegetation analysis method with nesting plots placed by purposive sampling and supported with environmental and soil data measurements to determine the factors that indicate the habitat preference of Ficus. We found 18 plant species of Ficus spp. in total. At Resort Pattunuang we found 14 Ficus species with the highest abundance being Ficus sundaica (27.55%), while we recorded 15 Ficus species at Resort Bantimurung with the highest abundance being Ficus ampelas (29.23%). Ficus species were uniformly distributed with a relatively high ratio of species composition in both resorts. Principal Component Analysis (PCA) showed that the presence of Ficus in Pattunuang Resort is influenced by wind speed, soil temperature, air temperature, soil moisture, soil pH, and air humidity. In Bantimurung Resort, the existence of Ficus is influenced by wind speed, soil temperature, soil moisture, and air humidity.
{"title":"Diversity and Distribution of Ficus (Moraceae) in The Karst Ecosystem of Bantimurung Bulusaraung National Park","authors":"Yelastri Yelastri, Sulistijorini Sulistijorini, Nina Ratna Djuita","doi":"10.22146/jtbb.78811","DOIUrl":"https://doi.org/10.22146/jtbb.78811","url":null,"abstract":"Bantimurung Bulusaraung National Park is an area that has the largest karst ecosystem in Indonesia. Karst is prone to damage and difficult to reuse, so it requires conservation efforts. One of the plant species that can maintain this sustainability is Ficus which acts as key species in karst ecosystems. However, at this time the species is experiencing disturbances, one of which is due to the experience of invasive plants that can threaten the existence of Ficus because the weeds can colonize habitats and are dominant which can change species diversity. This study aimed to analyze the diversity and distribution of Ficus, compare species composition, and analyze environmental factors that affect Ficus spp. in Pattunuang Resort and Bantimurung Resort. Vegetation analysis method with nesting plots placed by purposive sampling and supported with environmental and soil data measurements to determine the factors that indicate the habitat preference of Ficus. We found 18 plant species of Ficus spp. in total. At Resort Pattunuang we found 14 Ficus species with the highest abundance being Ficus sundaica (27.55%), while we recorded 15 Ficus species at Resort Bantimurung with the highest abundance being Ficus ampelas (29.23%). Ficus species were uniformly distributed with a relatively high ratio of species composition in both resorts. Principal Component Analysis (PCA) showed that the presence of Ficus in Pattunuang Resort is influenced by wind speed, soil temperature, air temperature, soil moisture, soil pH, and air humidity. In Bantimurung Resort, the existence of Ficus is influenced by wind speed, soil temperature, soil moisture, and air humidity. ","PeriodicalId":52402,"journal":{"name":"Journal of Tropical Biodiversity and Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48460654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dragonflies are insects that are very dependent on the existence of freshwater ecosystems, However, the population of dragonflies in urban freshwater ecosystems is at risk due to a number of issues. Consequently, it is essential to carry out research and efforts to preserve dragonflies in urban areas. This study aims to provide information about the diversity and structure of dragonfly communities in various habitat types in the Lakarsantri, Surabaya. Data collection in this study was carried out in the habitat types of ponds, reservoir, river, and rice field in July to September 2021. The study results show there are 22 species from 4 families with a total of 827 individuals. Analysis of the Shannon-Wiener diversity index showed that the highest value of dragonfly diversity was found in a pond, with a value of H' = 2.40, and the location with the lowest value was a river, with a value of H' = 1.77. At four research locations that have different aquatic ecosystems, the community structure of dragonfly is also different. The composition of the dragonfly community structure at the reservoir location has similarities to a pond, and at a river location, it has similarities to a paddy field. Differences in abiotic factors consisting of light intensity, humidity, and temperature at each study location have a correlation with differences in dragonfly community structure. In addition, the composition of the vegetation at each location is also one of the factors causing differences in the structure of the dragonfly community.
{"title":"Diversity and Community Structure of Dragonflies (Odonata) in Various Types of Habitat at Lakarsantri District, Surabaya, Indonesia","authors":"M. A. D. Susanto, N. F. Firdhausi, S. Bahri","doi":"10.22146/jtbb.76690","DOIUrl":"https://doi.org/10.22146/jtbb.76690","url":null,"abstract":"Dragonflies are insects that are very dependent on the existence of freshwater ecosystems, However, the population of dragonflies in urban freshwater ecosystems is at risk due to a number of issues. Consequently, it is essential to carry out research and efforts to preserve dragonflies in urban areas. This study aims to provide information about the diversity and structure of dragonfly communities in various habitat types in the Lakarsantri, Surabaya. Data collection in this study was carried out in the habitat types of ponds, reservoir, river, and rice field in July to September 2021. The study results show there are 22 species from 4 families with a total of 827 individuals. Analysis of the Shannon-Wiener diversity index showed that the highest value of dragonfly diversity was found in a pond, with a value of H' = 2.40, and the location with the lowest value was a river, with a value of H' = 1.77. At four research locations that have different aquatic ecosystems, the community structure of dragonfly is also different. The composition of the dragonfly community structure at the reservoir location has similarities to a pond, and at a river location, it has similarities to a paddy field. Differences in abiotic factors consisting of light intensity, humidity, and temperature at each study location have a correlation with differences in dragonfly community structure. In addition, the composition of the vegetation at each location is also one of the factors causing differences in the structure of the dragonfly community. ","PeriodicalId":52402,"journal":{"name":"Journal of Tropical Biodiversity and Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46986208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ledokombo hillocks are small hills located in Jember, East Java that have natural resources and face habitat alteration such as plantation and mining. However, a study of the diversity of butterflies has not been carried out in this area. We analysed the diversity of butterflies using the Shannon-Wiener diversity index (H’) and Pielou evenness index (E). We identified 514 individuals from 34 species and demonstrated a moderate diversity of butterflies (H'= 1.907) in this area. Our study results could be used for sustainable ecological management of plantations in Ledokombo Hillocks, Jember, East Java.
{"title":"Diversity of Butterflies in Ledokombo Hillocks Jember, East Java, Indonesia","authors":"Husni Mubarok, Alfina Eka Dwi Candra, Bayu Sandika, Wiwin Maisyaroh, Ellena Yusti, A. Qodri","doi":"10.22146/jtbb.77655","DOIUrl":"https://doi.org/10.22146/jtbb.77655","url":null,"abstract":"Ledokombo hillocks are small hills located in Jember, East Java that have natural resources and face habitat alteration such as plantation and mining. However, a study of the diversity of butterflies has not been carried out in this area. We analysed the diversity of butterflies using the Shannon-Wiener diversity index (H’) and Pielou evenness index (E). We identified 514 individuals from 34 species and demonstrated a moderate diversity of butterflies (H'= 1.907) in this area. Our study results could be used for sustainable ecological management of plantations in Ledokombo Hillocks, Jember, East Java. ","PeriodicalId":52402,"journal":{"name":"Journal of Tropical Biodiversity and Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41446967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-30DOI: 10.20873/jbb.uft.cemaf.v11n1.silva
Annita Ingrid Alves Silva, Celia Gomes de Siqueira
Os fungos filamentosos participam da produção de alimentos, nos produtos para saúde e na reciclagem de compostos da biosfera. Na indústria eles são utilizados na produção de enzimas, vitaminas, polissacarídeos, pigmentos, lipídios e glicolipídios, valiosos em biotecnologia. O Brasil possui grande diversidade biológica de paisagens, entre elas está a Caatinga, bastante explorada quanto ao potencial biotecnológico vegetal, mas com poucos estudos sobre o potencial dos microrganismos associados a esse bioma. Este estudo teve por objetivo avaliar a produção de enzimas de fungos isolados no solo associado a plantas do semi-árido. As amostras de solo foram coletadas na região da Grota do Angico, município de Poço Redondo, Sergipe, associadas a duas plantas características, Croton heliotropiifolius Kunth e Xiquexique gounellei. Foram isolados 39 fungos das amostras de solo e suas atividades proteolítica, amilolítica e pectinolítica foram avaliadas. Os 17 fungos associados à C. heliotropiifolius apresentam predominantemente atividade de proteases, sendo que apenas um deles apresentou atividade de pectinase, nos demais os resultados foram negativos. Por outro lado, um maior número dos 22 fungos isolados do solo associado à X. goiunellei, atividade enzimática e diversidade de enzimas, sendo que a maioria apresentou atividade proteolítica. Foi detectada atividade amilolítica em oito fungos, atividade proteolítica em 12 fungos e atividade pectinolítica em 6 fungos. Evidenciou-se o potencial dos fungos isolados do bioma da Caatinga para produção de enzimas, principalmente proteolíticas. Os fungos isolados no solo associado a plantas da Caatinga apresentam potencial para a produção de enzimas, principalmente proteolíticas.
丝状真菌参与食品生产、保健品和生物圈化合物的回收。在工业上，它们被用于生产酶、维生素、多糖、色素、脂类和糖脂，这些在生物技术中很有价值。巴西拥有丰富的生物多样性景观，其中包括Caatinga，它在植物生物技术潜力方面得到了广泛的开发，但对与该生物群落相关的微生物潜力的研究很少。本研究旨在评价半干旱植物土壤中分离真菌产生的酶。土壤样品是在Sergipe poco Redondo市的Grota do Angico地区采集的，与两种特色植物Croton heliotropiifolius Kunth和Xiquexique gounellei有关。从土壤样品中分离出39株真菌，并对其蛋白水解、淀粉水解和果胶水解活性进行了评价。17种与heliotropiifolius相关的真菌表现出主要的蛋白酶活性，其中只有1种表现出果胶酶活性，其他均为阴性。另一方面，22种与goiunellei相关的土壤真菌中，酶活性和酶多样性较高，其中大部分表现出蛋白水解活性。8种真菌具有淀粉酶活性，12种真菌具有蛋白水解活性，6种真菌具有果胶水解活性。从Caatinga生物群系分离的真菌具有生产酶，特别是蛋白水解酶的潜力。与Caatinga植物相关的土壤真菌具有生产酶的潜力，特别是蛋白水解酶。
{"title":"Diversidade enzimática de fungos associados a plantas da Caatinga","authors":"Annita Ingrid Alves Silva, Celia Gomes de Siqueira","doi":"10.20873/jbb.uft.cemaf.v11n1.silva","DOIUrl":"https://doi.org/10.20873/jbb.uft.cemaf.v11n1.silva","url":null,"abstract":"Os fungos filamentosos participam da produção de alimentos, nos produtos para saúde e na reciclagem de compostos da biosfera. Na indústria eles são utilizados na produção de enzimas, vitaminas, polissacarídeos, pigmentos, lipídios e glicolipídios, valiosos em biotecnologia. O Brasil possui grande diversidade biológica de paisagens, entre elas está a Caatinga, bastante explorada quanto ao potencial biotecnológico vegetal, mas com poucos estudos sobre o potencial dos microrganismos associados a esse bioma. Este estudo teve por objetivo avaliar a produção de enzimas de fungos isolados no solo associado a plantas do semi-árido. As amostras de solo foram coletadas na região da Grota do Angico, município de Poço Redondo, Sergipe, associadas a duas plantas características, Croton heliotropiifolius Kunth e Xiquexique gounellei. Foram isolados 39 fungos das amostras de solo e suas atividades proteolítica, amilolítica e pectinolítica foram avaliadas. Os 17 fungos associados à C. heliotropiifolius apresentam predominantemente atividade de proteases, sendo que apenas um deles apresentou atividade de pectinase, nos demais os resultados foram negativos. Por outro lado, um maior número dos 22 fungos isolados do solo associado à X. goiunellei, atividade enzimática e diversidade de enzimas, sendo que a maioria apresentou atividade proteolítica. Foi detectada atividade amilolítica em oito fungos, atividade proteolítica em 12 fungos e atividade pectinolítica em 6 fungos. Evidenciou-se o potencial dos fungos isolados do bioma da Caatinga para produção de enzimas, principalmente proteolíticas. Os fungos isolados no solo associado a plantas da Caatinga apresentam potencial para a produção de enzimas, principalmente proteolíticas.","PeriodicalId":52402,"journal":{"name":"Journal of Tropical Biodiversity and Biotechnology","volume":"49 6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89204865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-30DOI: 10.20873/jbb.uft.cemaf.v10n3.kobayasi
Tamiris Machado Kobayasi, Darío Abel Palmieri, M. R. Bertão
Pereskia aculeata Mill., conhecida como ora-pro-nobis, é uma planta alimentícia não convencional (PANC) que possui alto teor de proteínas, superando muitos outros vegetais utilizados habitualmente na alimentação humana. Além de ser fonte de proteínas, esta espécie apresenta compostos bioativos que lhe confere propriedades analgésicas, anti-inflamatórias e antioxidantes. Ora-pro-nobis apresenta uma mucilagem capaz de atuar como agente espessante, emulsificante e gelificante, características devidas à presença do biopolímero arabinogalactana e sua adesão às proteínas presentes na mucilagem. O presente estudo teve como objetivo a caracterização química e a determinação da atividade antioxidante da mucilagem em pó da ora-pro-nobis. A quantificação de proteínas demonstrou que a mucilagem liofilizada tem teor proteico maior do que em folhas frescas de ora-pro-nobis, apresentando média de 39,55 % de proteínas. A avaliação da atividade antioxidante demonstrou correlação positiva com o aumento da concentração da mucilagem e do teor de flavonoides totais.
{"title":"Caracterização química e atividade antioxidante da mucilagem em pó de ora-pro-nobis","authors":"Tamiris Machado Kobayasi, Darío Abel Palmieri, M. R. Bertão","doi":"10.20873/jbb.uft.cemaf.v10n3.kobayasi","DOIUrl":"https://doi.org/10.20873/jbb.uft.cemaf.v10n3.kobayasi","url":null,"abstract":"Pereskia aculeata Mill., conhecida como ora-pro-nobis, é uma planta alimentícia não convencional (PANC) que possui alto teor de proteínas, superando muitos outros vegetais utilizados habitualmente na alimentação humana. Além de ser fonte de proteínas, esta espécie apresenta compostos bioativos que lhe confere propriedades analgésicas, anti-inflamatórias e antioxidantes. Ora-pro-nobis apresenta uma mucilagem capaz de atuar como agente espessante, emulsificante e gelificante, características devidas à presença do biopolímero arabinogalactana e sua adesão às proteínas presentes na mucilagem. O presente estudo teve como objetivo a caracterização química e a determinação da atividade antioxidante da mucilagem em pó da ora-pro-nobis. A quantificação de proteínas demonstrou que a mucilagem liofilizada tem teor proteico maior do que em folhas frescas de ora-pro-nobis, apresentando média de 39,55 % de proteínas. A avaliação da atividade antioxidante demonstrou correlação positiva com o aumento da concentração da mucilagem e do teor de flavonoides totais.","PeriodicalId":52402,"journal":{"name":"Journal of Tropical Biodiversity and Biotechnology","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74959213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-30DOI: 10.20873/jbb.uft.cemaf.v11n1.aguiar
Claudio Lima, Alessandra Menegazzo Rosa
Few studies have been reported on lactic acid production by kefir grains. Kefir has been widely associated with probiotic use due to its cell growth in food matrices such as milks, juices and sugary solutions. However, from industrial scale there are no reports of its use in lactic acid production. In this work, we carried out experiments to test and understand how glucose, galactose and lactose, during lactic-acid fermentation, were converted in lactic acid by kefir grains. Given the microbial complexity in coexistence in kefir grains, it is likely that kinetic studies are not the most appropriate for the positive or negative definition of the kefir use as a starter in the lactic acid fermentative production on an industrial scale. It was concluded that, although with a higher Lag phase, lactose was the substrate that best presented a product and cell conversion rate, although glucose and galactose can also be used as a substrate in the production of this carboxylic acid.
{"title":"Behavior of the conversion of glucose, galactose and lactose to lactic acid by kefir grains","authors":"Claudio Lima, Alessandra Menegazzo Rosa","doi":"10.20873/jbb.uft.cemaf.v11n1.aguiar","DOIUrl":"https://doi.org/10.20873/jbb.uft.cemaf.v11n1.aguiar","url":null,"abstract":"Few studies have been reported on lactic acid production by kefir grains. Kefir has been widely associated with probiotic use due to its cell growth in food matrices such as milks, juices and sugary solutions. However, from industrial scale there are no reports of its use in lactic acid production. In this work, we carried out experiments to test and understand how glucose, galactose and lactose, during lactic-acid fermentation, were converted in lactic acid by kefir grains. Given the microbial complexity in coexistence in kefir grains, it is likely that kinetic studies are not the most appropriate for the positive or negative definition of the kefir use as a starter in the lactic acid fermentative production on an industrial scale. It was concluded that, although with a higher Lag phase, lactose was the substrate that best presented a product and cell conversion rate, although glucose and galactose can also be used as a substrate in the production of this carboxylic acid.","PeriodicalId":52402,"journal":{"name":"Journal of Tropical Biodiversity and Biotechnology","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83778553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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