Pub Date : 2023-03-12DOI: 10.24018/clinicmed.2023.4.2.223
C. Olivier, Daiana G. Pinto, Ana P. M. Teixeira, Jhéssica L. S. Santana, Raquel A. P. G. Santos, Regiane P. S. Lima
Background: The presence of anti-Saccharomyces cerevisiae antibodies (ASCA) has been reported in the serum of patients with several immune-inflammatory diseases. Objective: To evaluate the presence of ASCA in patients with non-IgE-mediated hypersensitivity. Methods: A group of 222 patients with non-IgE-mediated hypersensitivity was divided into three groups according to dermatologic, gastrointestinal, and respiratory symptoms. Group D was composed of 163 patients with dermatologic symptoms diagnosed as intrinsic atopic dermatitis and/or non-IgE-mediated urticaria. Group G was composed of 23 patients diagnosed with non-IgE-mediated gastrointestinal food allergies. Group R was composed of 36 patients with respiratory symptoms diagnosed as non-IgE-mediated rhinitis and/or non-IgE-mediated asthma. Results: The Wilcoxon-Mann-Whitney U test comparing the precipitin’s titers of group G and group D showed a non-significant p-value of 0.83366. The Wilcoxon-Mann-Whitney U test comparing the precipitin’s titers of group R and group G showed a significant p-value of 0.00034. The Wilcoxon-Mann-Whitney U test comparing the precipitin’s titers of group R and group D showed a significant p-value < 0.0001. Conclusion: The patients with respiratory symptoms diagnosed as non-IgE-mediated rhinitis and/or asthma presented significantly less humoral immunoreactivity against S. cerevisiae than patients with non-IgE-mediated food allergy and patients with intrinsic atopic dermatitis and/or non-IgE-mediated urticaria. The elevation of ASCA titers may be an unspecific marker of intestinal hyperpermeability, and possibly may participate in Gell and Coomb’s types II and/or type III hypersensitivity reactions responsible for the patient’s dermatologic and gastrointestinal symptoms.
{"title":"Anti-Saccharomyces cerevisiae Antibodies (ASCA) Researched by Tube Precipitins are Elevated in Patients with Dermatologic and Gastrointestinal Non-Ige-Mediated Hypersensitivity","authors":"C. Olivier, Daiana G. Pinto, Ana P. M. Teixeira, Jhéssica L. S. Santana, Raquel A. P. G. Santos, Regiane P. S. Lima","doi":"10.24018/clinicmed.2023.4.2.223","DOIUrl":"https://doi.org/10.24018/clinicmed.2023.4.2.223","url":null,"abstract":"Background: The presence of anti-Saccharomyces cerevisiae antibodies (ASCA) has been reported in the serum of patients with several immune-inflammatory diseases.\u0000Objective: To evaluate the presence of ASCA in patients with non-IgE-mediated hypersensitivity.\u0000Methods: A group of 222 patients with non-IgE-mediated hypersensitivity was divided into three groups according to dermatologic, gastrointestinal, and respiratory symptoms. Group D was composed of 163 patients with dermatologic symptoms diagnosed as intrinsic atopic dermatitis and/or non-IgE-mediated urticaria. Group G was composed of 23 patients diagnosed with non-IgE-mediated gastrointestinal food allergies. Group R was composed of 36 patients with respiratory symptoms diagnosed as non-IgE-mediated rhinitis and/or non-IgE-mediated asthma.\u0000Results: The Wilcoxon-Mann-Whitney U test comparing the precipitin’s titers of group G and group D showed a non-significant p-value of 0.83366. The Wilcoxon-Mann-Whitney U test comparing the precipitin’s titers of group R and group G showed a significant p-value of 0.00034. The Wilcoxon-Mann-Whitney U test comparing the precipitin’s titers of group R and group D showed a significant p-value < 0.0001.\u0000Conclusion: The patients with respiratory symptoms diagnosed as non-IgE-mediated rhinitis and/or asthma presented significantly less humoral immunoreactivity against S. cerevisiae than patients with non-IgE-mediated food allergy and patients with intrinsic atopic dermatitis and/or non-IgE-mediated urticaria. The elevation of ASCA titers may be an unspecific marker of intestinal hyperpermeability, and possibly may participate in Gell and Coomb’s types II and/or type III hypersensitivity reactions responsible for the patient’s dermatologic and gastrointestinal symptoms.","PeriodicalId":52409,"journal":{"name":"European Journal of Translational and Clinical Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82266836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-12DOI: 10.24018/clinicmed.2023.4.2.238
C. Olivier, Daiana G. Pinto, Ana P. M. Teixeira, Jhéssica L. S. Santana, Raquel A. P. G. Santos, R. P. S. Lima, Everton S. Monteiro
Background: The ancient concept of “intrinsic” and “extrinsic” asthma has evolved in line with growing scientific knowledge and the use of new clinical diagnostic tools. Objective: The present study aims to evaluate the non-IgE-mediated immunoreactivity against common allergens, in asthmatic patients without evidence of IgE-mediated hypersensitivity against these allergens. Methods: A group of 127 “intrinsic” asthmatic outpatients, were submitted to ex vivo Leukocyte Adherence Inhibition Tests (LAIT) with extracts of Dermatophagoides pteronyssinus, Hevea brasiliensis latex, dog dander, cat dander, cow’s milk proteins, beekeeping pollen and a mixture of fungal extracts. Results: Cascade graphs were assembled according to the distribution of the LAIT results among the range of results of Leukocyte Adherence Inhibition (LAI) inside each group. A column graph was plotted with the mean LAI results for each antigen. The non-parametric Wilcoxon-Mann-Whitney U-test performed between the results of each test was significant only between the results of the LAIT performed with the dog extract and the mixed fungal extract (U = 1,179.5; the z-score was -2.64109; the p-value was 0.0083). All other Wilcoxon-Mann-Whitney U-test calculations between LAIT performed with the other allergens were not significant at p < 0.01. Conclusion: The evidence of non-IgE-mediated immunoreactivity in allergic patients has been reported in the medical literature since the beginning of the 19th century and has been increasing since then. This descriptive article presents the contribution of the LAIT as a tool to quantify the overall non-IgE-mediated cellular/humoral immunoreactivity against common allergens in patients formerly classified inside the “intrinsic” asthma endotype.
{"title":"Evaluating Non-IgE-Mediated Allergens’ Immunoreactivity in Patients Formerly Classified as “Intrinsic” Asthmatics with Help of the Leukocyte Adherence Inhibition Test","authors":"C. Olivier, Daiana G. Pinto, Ana P. M. Teixeira, Jhéssica L. S. Santana, Raquel A. P. G. Santos, R. P. S. Lima, Everton S. Monteiro","doi":"10.24018/clinicmed.2023.4.2.238","DOIUrl":"https://doi.org/10.24018/clinicmed.2023.4.2.238","url":null,"abstract":"Background: The ancient concept of “intrinsic” and “extrinsic” asthma has evolved in line with growing scientific knowledge and the use of new clinical diagnostic tools.\u0000Objective: The present study aims to evaluate the non-IgE-mediated immunoreactivity against common allergens, in asthmatic patients without evidence of IgE-mediated hypersensitivity against these allergens.\u0000Methods: A group of 127 “intrinsic” asthmatic outpatients, were submitted to ex vivo Leukocyte Adherence Inhibition Tests (LAIT) with extracts of Dermatophagoides pteronyssinus, Hevea brasiliensis latex, dog dander, cat dander, cow’s milk proteins, beekeeping pollen and a mixture of fungal extracts.\u0000Results: Cascade graphs were assembled according to the distribution of the LAIT results among the range of results of Leukocyte Adherence Inhibition (LAI) inside each group. A column graph was plotted with the mean LAI results for each antigen. The non-parametric Wilcoxon-Mann-Whitney U-test performed between the results of each test was significant only between the results of the LAIT performed with the dog extract and the mixed fungal extract (U = 1,179.5; the z-score was -2.64109; the p-value was 0.0083). All other Wilcoxon-Mann-Whitney U-test calculations between LAIT performed with the other allergens were not significant at p < 0.01.\u0000Conclusion: The evidence of non-IgE-mediated immunoreactivity in allergic patients has been reported in the medical literature since the beginning of the 19th century and has been increasing since then. This descriptive article presents the contribution of the LAIT as a tool to quantify the overall non-IgE-mediated cellular/humoral immunoreactivity against common allergens in patients formerly classified inside the “intrinsic” asthma endotype.","PeriodicalId":52409,"journal":{"name":"European Journal of Translational and Clinical Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89766882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: The concept of giving out accolades and rewards has been there for centuries. When an individual is recognized for their good deed, they feel delighted. This inspires them to keep getting better and binds them to greater goals. First Annual award ceremony of the Mankweng Tertiary and Academic Hospital was held in March 2022. The hospital’s management recognized outstanding employees from all the different sections. After the ceremony, the hospital’s management displayed those award winners’ names on the wall of the main corridor of the hospital and this was done in order to enhance further motivation, engagement & encouragement. The main objective of the study to find out the employee’s perception on the displaying reward winner names in the wall of the Mankweng Hospital corridor and whether there is any enhancement of the employees' motivation. Material & Method: The questionnaires were distributed to the different units for all categories of workers in Mankweng Hospital. The questionnaires form comprises of 5 sections: Section A, B, C, D & E. The study was cross-sectional that applied one-way analysis of variance to assess participant’s responses, feeling and research is explanatory in nature. Result: Total 222 participants. Out of that 140 (63%) participants have positive feeling about the display the award winner name in the wall & 132 (59.5%) have participants are motivated with this display and in future 54% participants prefer combination of Supervisor & Peer colleague’s assessment for reward selection. Conclusion: From this study we can conclude that displaying of award winner name in the public place enhance motivation of employee. Majority of participants agreed with the combination of Supervisors and colleagues involvement for future assessment method.
{"title":"Psychological Impact on Mankweng Hospital Employees on Displaying the Names of Award Winners in the Hospital Corridor Wall and Its Effect on Enhancing Motivation and Performance","authors":"Mirza Mohamod Zahir Uddin Bhuiyan, Ruth Sipho Mlambo","doi":"10.24018/clinicmed.2023.4.2.257","DOIUrl":"https://doi.org/10.24018/clinicmed.2023.4.2.257","url":null,"abstract":"\u0000Background: The concept of giving out accolades and rewards has been there for centuries. When an individual is recognized for their good deed, they feel delighted. This inspires them to keep getting better and binds them to greater goals. First Annual award ceremony of the Mankweng Tertiary and Academic Hospital was held in March 2022. The hospital’s management recognized outstanding employees from all the different sections. After the ceremony, the hospital’s management displayed those award winners’ names on the wall of the main corridor of the hospital and this was done in order to enhance further motivation, engagement & encouragement. The main objective of the study to find out the employee’s perception on the displaying reward winner names in the wall of the Mankweng Hospital corridor and whether there is any enhancement of the employees' motivation. \u0000\u0000\u0000Material & Method: The questionnaires were distributed to the different units for all categories of workers in Mankweng Hospital. The questionnaires form comprises of 5 sections: Section A, B, C, D & E. The study was cross-sectional that applied one-way analysis of variance to assess participant’s responses, feeling and research is explanatory in nature. \u0000\u0000\u0000Result: Total 222 participants. Out of that 140 (63%) participants have positive feeling about the display the award winner name in the wall & 132 (59.5%) have participants are motivated with this display and in future 54% participants prefer combination of Supervisor & Peer colleague’s assessment for reward selection. \u0000\u0000\u0000Conclusion: From this study we can conclude that displaying of award winner name in the public place enhance motivation of employee. Majority of participants agreed with the combination of Supervisors and colleagues involvement for future assessment method.\u0000","PeriodicalId":52409,"journal":{"name":"European Journal of Translational and Clinical Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75919441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-12DOI: 10.24018/clinicmed.2023.4.2.251
Naureen Ali, F. Alalawi
Background: Vitamin D deficiency is common worldwide, but is particularly prevalent in the Middle Eastern region, where cultural and religious factors influence its incidence further. As patients with Systemic Lupus Erythematosus (SLE) avoid sun exposure and use medications that could potentially result in vitamin D deficiency, this problem becomes more apparent. Unfortunately, there are no data available on the prevalence of vitamin D deficiency among patients with SLE in our local area. Objective: To assess the prevalence of vitamin D deficiency among patients with a new onset of systemic lupus erythematosus in Dubai, and to determine whether low vitamin D levels are correlated with disease activity. Methodology and Data Collection: a retrospective cohort study of patients with new onset of Systemic Lupus Erythematosus, being diagnosed in less than 1 year and were followed up at Dubai Hospital (UAE) from January 2009 till December 2012. Demographic and clinical data were collected as well as 25(OH) D3 levels. The incidence of vitamin D insufficiency was calculated as a ratio between the numbers of patients with 25(OH) D levels below the specified cut-off values (30ng/ml and 10ng/ml, respectively). SLE was diagnosed according to the American college of rheumatology (ACR SLE) classification criteria of 1997. Patients with drug-induced SLE and vitamin D deficiency were excluded. Results: a total number of 150 patients were included. 87% (n=131) were females and 13% (n=19) were males. The mean vitamin D level at the baseline was 21.6+/- 47 (mean +/- SD), increased to 27.8+/- 16 at 1 year, while the SLEDAI has improved from 8.3+/-5 (mean +/- SD) to 2.4 +/- 2.9 at 1 year respectively. The changes in vitamin D level as well as the SLEDAI were statistically significant with a P value <0.001. Spearman correlation test showed a statistically significant inverse correlation between vitamin D level versus SLEDAI % from baseline to 1-year later. Conclusion: This study showed a direct inverse relationship between lupus activity and a low level of vitamin D. Furthermore, the SLE disease activity score got better with the improvement in vitamin D level, measured at baseline, and after 12 months period.
背景:维生素D缺乏症在世界范围内都很常见，但在中东地区尤为普遍，文化和宗教因素进一步影响了其发病率。由于系统性红斑狼疮(SLE)患者避免阳光照射并使用可能导致维生素D缺乏的药物，这一问题变得更加明显。不幸的是，在我们当地没有关于SLE患者中维生素D缺乏症患病率的数据。目的:评估迪拜新发系统性红斑狼疮患者维生素D缺乏症的患病率，并确定低维生素D水平是否与疾病活动度相关。方法和数据收集:回顾性队列研究，2009年1月至2012年12月在迪拜医院(阿联酋)新发系统性红斑狼疮患者，诊断时间少于1年，随访。收集人口统计学和临床数据以及25(OH) D3水平。维生素D不足的发生率计算为25(OH) D水平低于指定临界值(分别为30ng/ml和10ng/ml)的患者人数之间的比率。SLE诊断依据1997年美国风湿病学会(American college of rheumatology, ACR) SLE分类标准。排除药物性SLE和维生素D缺乏症患者。结果:共纳入150例患者。87% (n=131)为女性，13% (n=19)为男性。基线时的平均维生素D水平为21.6+/- 47(平均+/- SD)， 1年后增加到27.8+/- 16,SLEDAI分别从8.3+/-5(平均+/- SD)提高到2.4 +/- 2.9。维生素D水平及SLEDAI变化均有统计学意义，P值<0.001。Spearman相关检验显示，从基线到1年后，维生素D水平与SLEDAI %呈显著负相关。结论:本研究显示狼疮活动性与低水平维生素D之间存在直接的反比关系。此外，在基线和12个月后测量的狼疮疾病活动性评分随着维生素D水平的提高而改善。
{"title":"Vitamin D Status and SLE Disease Activity among Dubai Hospital Lupus Patients","authors":"Naureen Ali, F. Alalawi","doi":"10.24018/clinicmed.2023.4.2.251","DOIUrl":"https://doi.org/10.24018/clinicmed.2023.4.2.251","url":null,"abstract":"\u0000Background: Vitamin D deficiency is common worldwide, but is particularly prevalent in the Middle Eastern region, where cultural and religious factors influence its incidence further. As patients with Systemic Lupus Erythematosus (SLE) avoid sun exposure and use medications that could potentially result in vitamin D deficiency, this problem becomes more apparent. Unfortunately, there are no data available on the prevalence of vitamin D deficiency among patients with SLE in our local area.\u0000\u0000\u0000Objective: To assess the prevalence of vitamin D deficiency among patients with a new onset of systemic lupus erythematosus in Dubai, and to determine whether low vitamin D levels are correlated with disease activity.\u0000\u0000\u0000Methodology and Data Collection: a retrospective cohort study of patients with new onset of Systemic Lupus Erythematosus, being diagnosed in less than 1 year and were followed up at Dubai Hospital (UAE) from January 2009 till December 2012. Demographic and clinical data were collected as well as 25(OH) D3 levels. The incidence of vitamin D insufficiency was calculated as a ratio between the numbers of patients with 25(OH) D levels below the specified cut-off values (30ng/ml and 10ng/ml, respectively). SLE was diagnosed according to the American college of rheumatology (ACR SLE) classification criteria of 1997. Patients with drug-induced SLE and vitamin D deficiency were excluded.\u0000\u0000\u0000Results: a total number of 150 patients were included. 87% (n=131) were females and 13% (n=19) were males. The mean vitamin D level at the baseline was 21.6+/- 47 (mean +/- SD), increased to 27.8+/- 16 at 1 year, while the SLEDAI has improved from 8.3+/-5 (mean +/- SD) to 2.4 +/- 2.9 at 1 year respectively. The changes in vitamin D level as well as the SLEDAI were statistically significant with a P value <0.001. Spearman correlation test showed a statistically significant inverse correlation between vitamin D level versus SLEDAI % from baseline to 1-year later. \u0000\u0000\u0000Conclusion: This study showed a direct inverse relationship between lupus activity and a low level of vitamin D. Furthermore, the SLE disease activity score got better with the improvement in vitamin D level, measured at baseline, and after 12 months period.\u0000","PeriodicalId":52409,"journal":{"name":"European Journal of Translational and Clinical Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78093190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-12DOI: 10.24018/clinicmed.2023.4.2.237
I. G. A. P. Wahyu Widiantari, L. M. Ratnawati
Introduction: Rhinosinusitis is an inflammation of the nasal mucosa and paranasal sinuses. Acute rhinosinusitis that is not treated properly can progress to chronic rhinosinusitis. Chronic rhinosinusitis can lead to impaired quality of life. Until now there is no data regarding the relationship of TNSS to the quality of life of patients with chronic rhinosinusitis based on SNOT-22, therefore researchers want to conduct research on the relationship of TNSS to the quality of life of patients with chronic rhinosinusitis based on SNOT-22. Methods: This study was a retrospective analytical study with a cross-sectional study design. Results: There was a significant relationship with a strong correlation (r=0.724) between TNSS and the quality of life of chronic rhinosinusitis patients. Conclusion: There was a significant relationship with a strong correlation (r=0.724) between TNSS and the quality of life of chronic rhinosinusitis patients at Prof. Dr. I.G.N.G Ngoerah General Hospital Denpasar.
{"title":"Correlation Between Total Nasal Symptoms Score (TNSS) and Quality of Life of Chronic Rhinosinusitis Patients in ENT Outpatient Clinic of Prof. Dr. I.G.N.G Ngoerah General Hospital Denpasar in 2021","authors":"I. G. A. P. Wahyu Widiantari, L. M. Ratnawati","doi":"10.24018/clinicmed.2023.4.2.237","DOIUrl":"https://doi.org/10.24018/clinicmed.2023.4.2.237","url":null,"abstract":"\u0000Introduction: Rhinosinusitis is an inflammation of the nasal mucosa and paranasal sinuses. Acute rhinosinusitis that is not treated properly can progress to chronic rhinosinusitis. Chronic rhinosinusitis can lead to impaired quality of life. Until now there is no data regarding the relationship of TNSS to the quality of life of patients with chronic rhinosinusitis based on SNOT-22, therefore researchers want to conduct research on the relationship of TNSS to the quality of life of patients with chronic rhinosinusitis based on SNOT-22.\u0000\u0000\u0000Methods: This study was a retrospective analytical study with a cross-sectional study design. \u0000\u0000\u0000Results: There was a significant relationship with a strong correlation (r=0.724) between TNSS and the quality of life of chronic rhinosinusitis patients.\u0000\u0000\u0000Conclusion: There was a significant relationship with a strong correlation (r=0.724) between TNSS and the quality of life of chronic rhinosinusitis patients at Prof. Dr. I.G.N.G Ngoerah General Hospital Denpasar.\u0000","PeriodicalId":52409,"journal":{"name":"European Journal of Translational and Clinical Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81337938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-05DOI: 10.24018/clinicmed.2023.4.2.241
M. Ahmed, Sarina Koilpillai, Azeem R. Khan, Ehsan Rafiq, S. Carlan
Morbid obesity is a major health problem worldwide and the Roux-en-Y gastric bypass (RYGB) is a popular surgical treatment. A 40 year old woman, 17 years post RYGB presented with concurrent symptomatic gastric ulcers and acute pancreatitis. The diagnosis was difficult and after failed medical therapy, surgery was required. The gastric remnant is not easily accessible by endoscopy thus; detecting PUD in this excluded segment is difficult. As a result, alternative methods, such as exploratory laparotomy or pediatric endoscopy may be required to effectively diagnose the gastric remnant.
{"title":"A Rare Case of Perforated Gastric Remnant after Roux-enY Gastric Bypass","authors":"M. Ahmed, Sarina Koilpillai, Azeem R. Khan, Ehsan Rafiq, S. Carlan","doi":"10.24018/clinicmed.2023.4.2.241","DOIUrl":"https://doi.org/10.24018/clinicmed.2023.4.2.241","url":null,"abstract":"\u0000Morbid obesity is a major health problem worldwide and the Roux-en-Y gastric bypass (RYGB) is a popular surgical treatment. A 40 year old woman, 17 years post RYGB presented with concurrent symptomatic gastric ulcers and acute pancreatitis. The diagnosis was difficult and after failed medical therapy, surgery was required. The gastric remnant is not easily accessible by endoscopy thus; detecting PUD in this excluded segment is difficult. As a result, alternative methods, such as exploratory laparotomy or pediatric endoscopy may be required to effectively diagnose the gastric remnant.\u0000","PeriodicalId":52409,"journal":{"name":"European Journal of Translational and Clinical Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82446686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-23DOI: 10.24018/clinicmed.2023.4.1.240
G. Bailo, F. M. Rodríguez, C. Carabajal-Miotti, Susana G. Ruiz De Frattari, A. Vargas, N. E. González-Silva, I. Novak
Background: The formation of extracellular traps (ETs) as a microbicidal functional mechanism of various leukocytes has taken on importance today and they are implicated in the pathogenesis of various diseases and have even been involved in the current pandemic. Objectives: Study ETs generated in vitro from healthy human blood leukocytes against different stimuli (LPS, fMLP) and their influence on different T cells profiles and monocyte-macrophages, in autologous cell cultures. Methods: Heparinized human blood samples were collected with ethical consent. ETs generation was performed by stimulation with LPS and fMLP. Subsequently they were isolated. ETs influence on cell profile differentiation was performed in samples without stimulation, with OVA addition samples, and OVA-ETs addition samples. This assay was observed through immunofluorescence (IF) labeling of molecules of T CD4 profile; Th17 and innate lymphoid cells 3 ILC3 by RORɣ; activation status of T cells by CD45RO; and M1 macrophage profile by iNOS. Results: Significant CD4 and CD45RO positive cells percentage were observed between paired control samples and OVA addition samples (p <0.05), between paired control samples and OVA-ETs addition samples (p <0.05). In an independent experiment, significant differences were observed between OVA addition samples vs. OVA-ETs addition samples (p <0.05). At 72 h of culture, no significant differences were found between the paired samples in any case. There were no significant differences between paired control samples and OVA addition samples or OVA-ETs samples, neither 24 and 72 h of culture in RORɣ positive cells percentage or iNOS positive cells percentage. Conclusions: Influence of ETs on T cell activation was observed and components of autologous ETs did not elicit classical activation of M1 macrophages.
{"title":"Influence of Extracellular Traps (ETs) on the Differentiation of TCD4 Cell Profiles and Macrophages in Human Autologous Culture","authors":"G. Bailo, F. M. Rodríguez, C. Carabajal-Miotti, Susana G. Ruiz De Frattari, A. Vargas, N. E. González-Silva, I. Novak","doi":"10.24018/clinicmed.2023.4.1.240","DOIUrl":"https://doi.org/10.24018/clinicmed.2023.4.1.240","url":null,"abstract":"\u0000Background: The formation of extracellular traps (ETs) as a microbicidal functional mechanism of various leukocytes has taken on importance today and they are implicated in the pathogenesis of various diseases and have even been involved in the current pandemic. \u0000\u0000\u0000Objectives: Study ETs generated in vitro from healthy human blood leukocytes against different stimuli (LPS, fMLP) and their influence on different T cells profiles and monocyte-macrophages, in autologous cell cultures. \u0000\u0000\u0000Methods: Heparinized human blood samples were collected with ethical consent. ETs generation was performed by stimulation with LPS and fMLP. Subsequently they were isolated. ETs influence on cell profile differentiation was performed in samples without stimulation, with OVA addition samples, and OVA-ETs addition samples. This assay was observed through immunofluorescence (IF) labeling of molecules of T CD4 profile; Th17 and innate lymphoid cells 3 ILC3 by RORɣ; activation status of T cells by CD45RO; and M1 macrophage profile by iNOS. \u0000\u0000\u0000Results: Significant CD4 and CD45RO positive cells percentage were observed between paired control samples and OVA addition samples (p <0.05), between paired control samples and OVA-ETs addition samples (p <0.05). In an independent experiment, significant differences were observed between OVA addition samples vs. OVA-ETs addition samples (p <0.05). At 72 h of culture, no significant differences were found between the paired samples in any case. There were no significant differences between paired control samples and OVA addition samples or OVA-ETs samples, neither 24 and 72 h of culture in RORɣ positive cells percentage or iNOS positive cells percentage. \u0000\u0000\u0000Conclusions: Influence of ETs on T cell activation was observed and components of autologous ETs did not elicit classical activation of M1 macrophages.\u0000","PeriodicalId":52409,"journal":{"name":"European Journal of Translational and Clinical Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89395285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-23DOI: 10.24018/clinicmed.2023.4.1.245
C. Amadi, S. Lawson, Joy I. Nyeche, Inichinbia Boniface, Wala T. Kelachi, Emmanuel M. Owamagbe, Nkeiruka J. Amadi, Azubuike Ogba, Solomon Obioha, C. J. Okafor
Background: Altered hepatobiliary status has been reported in association with COVID-19 which has been linked, with limited evidence, to the exaggerated COVID-19-induced hyper-inflammatory response. Hence, the current study evaluated hepatocellular status and its association with indicators of inflammation among COVID-19 patients. Methods: This study was conducted among the RT-PCR-confirmed and treatment-naïve COVID-19 patients in Port Harcourt, South-south Nigeria. Pre-medical/surgical data were retrieved retrospectively from archived patients’ case notes, medical review charts, nurses’ vital signs/medication sheets, and laboratory records at the center using validated data acquisition templates. All retrieved data were analyzed using standard protocols. Results: Among the 396 studied, 16.7% (n=66) had hepatobiliary pathologies. The majority of those with hepatobiliary pathologies had severe COVID-19 (93.3%). Patients with severe COVID-19 and concurrent hepatobiliary pathologies were mostly males and of older age. Cholestatic-specific pathology was the most common pattern observed among the general cases with hepatobiliary pathologies and among those having specific mild, moderate, and severe COVID-19. Among those with severe COVID-19, significant positive relationships were observed between markers of inflammation (Proclacitonin/C-reactive protein/D-dimer) and cholestatic-specific hepatobiliary markers (ALP/G-GT/TBil/CBil) (p<0.05), but not with the hepatocellular-specific markers (ALT/AST) (p>0.05). In contrast, no significant relationship existed between the relevant markers of inflammation and all the cholestatic/hepatocellular markers among those with mild and moderate COVID-19 (p>0.05). Conclusion: Hepatobiliary pathologies, mostly of cholestatic patterns, are frequent among the studied COVID-19 patients which were associated with inflammatory markers among those with severe disease. Therefore, hepatobiliary evaluation should be prioritized, especially among those with severe COVID-19.
背景:据报道，肝胆状况的改变与COVID-19有关，但证据有限，这与COVID-19诱导的过度炎症反应有关。因此，本研究评估了COVID-19患者的肝细胞状态及其与炎症指标的关系。方法:本研究在尼日利亚南南哈科特港的rt - pcr确诊和treatment-naïve COVID-19患者中进行。使用经过验证的数据获取模板，从存档的患者病例记录、医学回顾表、护士生命体征/用药表和中心的实验室记录中回顾性检索医疗前/手术数据。所有检索到的数据使用标准协议进行分析。结果:在396例研究中，16.7% (n=66)有肝胆病变。肝胆病变患者以重症占多数(93.3%)。重症COVID-19合并肝胆病变患者多为男性，年龄较大。胆汁特异性病理是一般肝胆病理患者和特异性轻、中、重度COVID-19患者中最常见的病理模式。在重症患者中，炎症标志物(促乳素/ c反应蛋白/ d -二聚体)与胆汁淤积特异性肝胆标志物(ALP/G-GT/TBil/CBil)呈显著正相关(p0.05)。相比之下，轻、中度COVID-19患者炎症相关标志物与所有胆汁淤积/肝细胞标志物之间无显著相关性(p < 0.05)。结论:所研究的COVID-19患者中肝胆病变多为胆汁淤积型，重症患者中与炎症标志物相关。因此，应优先进行肝胆评估，特别是在COVID-19重症患者中。
{"title":"Patterns of Hepatobiliary Pathologies and Their Relationship with Markers of Inflammation in COVID-19 Patients","authors":"C. Amadi, S. Lawson, Joy I. Nyeche, Inichinbia Boniface, Wala T. Kelachi, Emmanuel M. Owamagbe, Nkeiruka J. Amadi, Azubuike Ogba, Solomon Obioha, C. J. Okafor","doi":"10.24018/clinicmed.2023.4.1.245","DOIUrl":"https://doi.org/10.24018/clinicmed.2023.4.1.245","url":null,"abstract":"\u0000Background: Altered hepatobiliary status has been reported in association with COVID-19 which has been linked, with limited evidence, to the exaggerated COVID-19-induced hyper-inflammatory response. Hence, the current study evaluated hepatocellular status and its association with indicators of inflammation among COVID-19 patients. \u0000\u0000\u0000Methods: This study was conducted among the RT-PCR-confirmed and treatment-naïve COVID-19 patients in Port Harcourt, South-south Nigeria. Pre-medical/surgical data were retrieved retrospectively from archived patients’ case notes, medical review charts, nurses’ vital signs/medication sheets, and laboratory records at the center using validated data acquisition templates. All retrieved data were analyzed using standard protocols.\u0000\u0000\u0000Results: Among the 396 studied, 16.7% (n=66) had hepatobiliary pathologies. The majority of those with hepatobiliary pathologies had severe COVID-19 (93.3%). Patients with severe COVID-19 and concurrent hepatobiliary pathologies were mostly males and of older age. Cholestatic-specific pathology was the most common pattern observed among the general cases with hepatobiliary pathologies and among those having specific mild, moderate, and severe COVID-19. Among those with severe COVID-19, significant positive relationships were observed between markers of inflammation (Proclacitonin/C-reactive protein/D-dimer) and cholestatic-specific hepatobiliary markers (ALP/G-GT/TBil/CBil) (p<0.05), but not with the hepatocellular-specific markers (ALT/AST) (p>0.05). In contrast, no significant relationship existed between the relevant markers of inflammation and all the cholestatic/hepatocellular markers among those with mild and moderate COVID-19 (p>0.05). \u0000\u0000\u0000Conclusion: Hepatobiliary pathologies, mostly of cholestatic patterns, are frequent among the studied COVID-19 patients which were associated with inflammatory markers among those with severe disease. Therefore, hepatobiliary evaluation should be prioritized, especially among those with severe COVID-19.\u0000","PeriodicalId":52409,"journal":{"name":"European Journal of Translational and Clinical Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82400772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nadia Dzierżanowska, M. Krakowiak, P. Sokal, Barbara Myszkowska
Background: For about 20 years we have been observing the development of three-dimensional (3D) printing. The aim of this study was to systematize the current knowledge on the use of 3D printing technology in neu - rosurgery and to attempt an outline the future paths of its development. Material and methods: The analysis was based on English-language literature from 2017-2021 indexed in the Mendeley and Scopus databases. Results: The application of 3D printing in neurosurgery concerns: 1) teaching students, 2) training of residents neurosurgeons, 3) individualized surgery planning, 4) dedicated cranial and spinal implants, 5) the future of 3D printing in neurosurgical implantology. There were 5 main neurosurgery subtopics in which 3D printing was used: “vascular neurosurgery” (31%), “skull, cranial neurosurgery” (22.4%), “neuro-oncology” (19.3%), “spine” (14.3%) and “others” (13%). The number of published articles has been steadily increasing by 11-33% annually. Conclusion: 3D printing has an enormous potential for clinical use and in the we will continue to observe its dynamic development. In neurosurgery 3D prints are currently most commonly used for didactic purposes as detailed anatomical models, for training residents and young surgeons and by specialists for the simulation of complex or innovative surgeries. The future of the use of additive 3D printing in neurosurgery lies in the biologi - cal 3D printing, the creation of artificial organs and the development of biological implants in tissue engineering.
{"title":"The application of 3D printing in neurosurgery: present and future","authors":"Nadia Dzierżanowska, M. Krakowiak, P. Sokal, Barbara Myszkowska","doi":"10.31373/ejtcm/158565","DOIUrl":"https://doi.org/10.31373/ejtcm/158565","url":null,"abstract":"Background: For about 20 years we have been observing the development of three-dimensional (3D) printing. The aim of this study was to systematize the current knowledge on the use of 3D printing technology in neu - rosurgery and to attempt an outline the future paths of its development. Material and methods: The analysis was based on English-language literature from 2017-2021 indexed in the Mendeley and Scopus databases. Results: The application of 3D printing in neurosurgery concerns: 1) teaching students, 2) training of residents neurosurgeons, 3) individualized surgery planning, 4) dedicated cranial and spinal implants, 5) the future of 3D printing in neurosurgical implantology. There were 5 main neurosurgery subtopics in which 3D printing was used: “vascular neurosurgery” (31%), “skull, cranial neurosurgery” (22.4%), “neuro-oncology” (19.3%), “spine” (14.3%) and “others” (13%). The number of published articles has been steadily increasing by 11-33% annually. Conclusion: 3D printing has an enormous potential for clinical use and in the we will continue to observe its dynamic development. In neurosurgery 3D prints are currently most commonly used for didactic purposes as detailed anatomical models, for training residents and young surgeons and by specialists for the simulation of complex or innovative surgeries. The future of the use of additive 3D printing in neurosurgery lies in the biologi - cal 3D printing, the creation of artificial organs and the development of biological implants in tissue engineering.","PeriodicalId":52409,"journal":{"name":"European Journal of Translational and Clinical Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45958424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-24DOI: 10.24018/clinicmed.2023.4.1.242
S. Gummaluri, S. R G, Bharti Chaudhary, Hirak S. Bhattacharya, R. Boyapati, Sheema Tasneem Mohammad
Background: Maintaining good oral health plays crucial in upholding one’s general health in a good shape. Present study has aimed to evaluate the knowledge of the above statement and awareness, and its relation to its general health among medical doctors. Materials and Methods: The study encompasses of 400 medical doctors (Clinical and Non-Clinical) having > 26 years of age. A questionnaire proforma, having 18 questions was provided to the participants and answered data was gathered. Further, collected data was subjected to statistical analysis. Frequency distribution percentages were calculated and depicted. Results: In case of periodontal awareness, significance difference was obtained between the non-clinical and clinical doctors regarding right brushing technique (p=0.048) favouring clinical doctors and dental visit for every 3 months favoured non-clinical doctors (p<0.001) while rest of the questions didn’t show any significant difference. Regarding periodontal knowledge, significant difference between clinical and non-clinical doctors were recorded in poor oral hygiene affecting the general health (p=0.0304) whereas rest of the parameters were non-significant. Conclusion: Conducting periodic dental awareness programs among medical doctors would be beneficial for early detection of dental problems and treating them at appropriate time. So that local infections nidus would not become systemic problems.
{"title":"Evaluation of Knowledge and Awareness of Periodontal Health and its relation to General Health among Medical Doctors-A Cross-Sectional Questionnaire Survey","authors":"S. Gummaluri, S. R G, Bharti Chaudhary, Hirak S. Bhattacharya, R. Boyapati, Sheema Tasneem Mohammad","doi":"10.24018/clinicmed.2023.4.1.242","DOIUrl":"https://doi.org/10.24018/clinicmed.2023.4.1.242","url":null,"abstract":"\u0000Background: Maintaining good oral health plays crucial in upholding one’s general health in a good shape. Present study has aimed to evaluate the knowledge of the above statement and awareness, and its relation to its general health among medical doctors. \u0000\u0000\u0000Materials and Methods: The study encompasses of 400 medical doctors (Clinical and Non-Clinical) having > 26 years of age. A questionnaire proforma, having 18 questions was provided to the participants and answered data was gathered. Further, collected data was subjected to statistical analysis. Frequency distribution percentages were calculated and depicted. \u0000\u0000\u0000Results: In case of periodontal awareness, significance difference was obtained between the non-clinical and clinical doctors regarding right brushing technique (p=0.048) favouring clinical doctors and dental visit for every 3 months favoured non-clinical doctors (p<0.001) while rest of the questions didn’t show any significant difference. Regarding periodontal knowledge, significant difference between clinical and non-clinical doctors were recorded in poor oral hygiene affecting the general health (p=0.0304) whereas rest of the parameters were non-significant.\u0000Conclusion: Conducting periodic dental awareness programs among medical doctors would be beneficial for early detection of dental problems and treating them at appropriate time. So that local infections nidus would not become systemic problems.\u0000","PeriodicalId":52409,"journal":{"name":"European Journal of Translational and Clinical Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75263526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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