M. Taheri, S. Riahi, Mohammad Kazem Emami Meybodi, M. Ahmadian-Attari, S. Esmaeili, R. Mokaberinejad
Osteoarthritis (OA) of the knee is a major health problem in the society. Iranian Traditional Medicine (ITM) or Persian Medicine (PM) as a branch of complementary medicine has been practiced in Iran for many centuries. An herbal medication known as squill oxymel has been used by PM physicians for OA. Our aim is to investigate the effect of squill oxymel on OA of the knee joint. Eighty eight patients were assigned to receive a placebo or squill oxymel syrup (10 ml each morning on empty stomach) for 8 consecutive weeks. Acetaminophen tablets were considered as the rescue medicine. Ultimately, 43 patients in the placebo group and 40 patients in the treatment group completed the trial and were included in the statistical analysis. Patients were followed for 4 weeks after cessation of treatment. The Knee injury and Osteoarthritis Outcome Score (KOOS) questionnaire and Visual Analog Scale (VAS) were considered as the main outcome measures. Laboratory tests including AST, ALT, BUN, Cr plus inflammatory tests including WBC, ESR, and CRP with specific tests i.e. interleukin 6 (IL6) and superoxide dismutase (SOD) at the beginning and the end of intervention were measured. The results showed the positive effect of treatment on the outcome of knee pain (p=0.04) and daily activity (p=0.01) of KOOS after Cessation of treatment. On the other hand, VAS decreased in both treatment and placebo groups; while it showed significance intra-group and showed no significance between the two groups. After 4 weeks of cessation of treatment, the positive effect of the squill oxymel on the treatment group continued in some of the subscales of KOOS, including symptoms, knee pain and daily activities, but stopped in the placebo group. In general, both clinically and statistically significant improvement was observed after cessation of treatment. Squill oxymel syrup showed promising results in management of knee OA but future researches with larger sample size and longer duration are necessary.
{"title":"Effect of Squill Oxymel on Knee Osteoarthritis: A Triple-Blind, Randomized, Controlled Clinical Trial","authors":"M. Taheri, S. Riahi, Mohammad Kazem Emami Meybodi, M. Ahmadian-Attari, S. Esmaeili, R. Mokaberinejad","doi":"10.18502/tim.v8i2.13077","DOIUrl":"https://doi.org/10.18502/tim.v8i2.13077","url":null,"abstract":"Osteoarthritis (OA) of the knee is a major health problem in the society. Iranian Traditional Medicine (ITM) or Persian Medicine (PM) as a branch of complementary medicine has been practiced in Iran for many centuries. An herbal medication known as squill oxymel has been used by PM physicians for OA. Our aim is to investigate the effect of squill oxymel on OA of the knee joint. Eighty eight patients were assigned to receive a placebo or squill oxymel syrup (10 ml each morning on empty stomach) for 8 consecutive weeks. Acetaminophen tablets were considered as the rescue medicine. Ultimately, 43 patients in the placebo group and 40 patients in the treatment group completed the trial and were included in the statistical analysis. Patients were followed for 4 weeks after cessation of treatment. The Knee injury and Osteoarthritis Outcome Score (KOOS) questionnaire and Visual Analog Scale (VAS) were considered as the main outcome measures. Laboratory tests including AST, ALT, BUN, Cr plus inflammatory tests including WBC, ESR, and CRP with specific tests i.e. interleukin 6 (IL6) and superoxide dismutase (SOD) at the beginning and the end of intervention were measured. The results showed the positive effect of treatment on the outcome of knee pain (p=0.04) and daily activity (p=0.01) of KOOS after Cessation of treatment. On the other hand, VAS decreased in both treatment and placebo groups; while it showed significance intra-group and showed no significance between the two groups. After 4 weeks of cessation of treatment, the positive effect of the squill oxymel on the treatment group continued in some of the subscales of KOOS, including symptoms, knee pain and daily activities, but stopped in the placebo group. In general, both clinically and statistically significant improvement was observed after cessation of treatment. Squill oxymel syrup showed promising results in management of knee OA but future researches with larger sample size and longer duration are necessary.","PeriodicalId":52659,"journal":{"name":"Traditional and Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46097635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Anxiety disorders are the most common mental disorders in communities. This cross-sectional study assessed the relationship between temperament and other demographic characteristics of participants with anxiety. It also determined the predictors of anxiety disorders among this cohort. This cross-sectional study was conducted in Kerman, Iran from 11 Mars, 2018 to 22 May, 2019. The participants were 1532 people who were selected through convenience sampling from those who attended the second round of KERCARDS in 2017. Physical activity, anxiety, depression, and temperament were assessed through related questionnaires. The collected data were described and analyzed by mean (Standard Deviation (SD)) and logistic regression models in SPSS v.23. The mean (SD) age of the participants was 30.02 (6.83) years (age range: 11 to 40). More than two-thirds of them were married (n=1084, 70.7%) and held a diploma and university degrees (n=1112, 72.6%). The anxiety symptoms were prevalent among more than one-third of the participants (n=574, 37.5%, 95%CI: 35.2, 40.1). According to multivariate logistic regression model, female sex (OR:1.80, 95%CI:1.41, 2.30; P value:0.001), illiteracy, lower education (OR:1.40, 95%CI:1.09, 1.81; P value:0.009), a history of depression (OR:14.51, 95%CI:9.65, 21.80; P value:0.001), and having warm Mizaj (OR:1.31, 95%CI:1, 1.71; P value:0.04) were determined as predictors of anxiety. In the present study, female sex, lower educational status, the experience of depression, and warm temperament increased the risk of anxiety and were determined as predictors of anxiety disorder. Diagnosis of temperament seems to be necessary for recognizing anxiety symptoms and also finding an effective treatment.
{"title":"The Warm Temperament (Mizaj) as a Predictor of Anxiety Disorder among Participants in the Kerman Coronary Artery Disease Risk Factors Study (KERCADRS)","authors":"Haleh Tajadini, Maliheh Sadat Bazrafshani, Mohadeseh Kamali","doi":"10.18502/tim.v8i2.13079","DOIUrl":"https://doi.org/10.18502/tim.v8i2.13079","url":null,"abstract":"Anxiety disorders are the most common mental disorders in communities. This cross-sectional study assessed the relationship between temperament and other demographic characteristics of participants with anxiety. It also determined the predictors of anxiety disorders among this cohort. This cross-sectional study was conducted in Kerman, Iran from 11 Mars, 2018 to 22 May, 2019. The participants were 1532 people who were selected through convenience sampling from those who attended the second round of KERCARDS in 2017. Physical activity, anxiety, depression, and temperament were assessed through related questionnaires. The collected data were described and analyzed by mean (Standard Deviation (SD)) and logistic regression models in SPSS v.23. The mean (SD) age of the participants was 30.02 (6.83) years (age range: 11 to 40). More than two-thirds of them were married (n=1084, 70.7%) and held a diploma and university degrees (n=1112, 72.6%). The anxiety symptoms were prevalent among more than one-third of the participants (n=574, 37.5%, 95%CI: 35.2, 40.1). According to multivariate logistic regression model, female sex (OR:1.80, 95%CI:1.41, 2.30; P value:0.001), illiteracy, lower education (OR:1.40, 95%CI:1.09, 1.81; P value:0.009), a history of depression (OR:14.51, 95%CI:9.65, 21.80; P value:0.001), and having warm Mizaj (OR:1.31, 95%CI:1, 1.71; P value:0.04) were determined as predictors of anxiety. In the present study, female sex, lower educational status, the experience of depression, and warm temperament increased the risk of anxiety and were determined as predictors of anxiety disorder. Diagnosis of temperament seems to be necessary for recognizing anxiety symptoms and also finding an effective treatment.","PeriodicalId":52659,"journal":{"name":"Traditional and Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44273581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Qodsiye Hosseinifakhr, Yasaman Vahedi-Mazdabadi, Mina Saeedi, M. S. Shams Ardekani
Gender selection has emerged as a controversial issue in different countries and in this respect, a wide range of studies and international symposiums have been dedicated to this subject. A comprehensive literature review was performed without restriction on publication date. Also, the credible Persian Medicine (PM) resources such as "Exir-e-Azam" (The Great Elixir), "Qarabadin-e-Kabir" (Great Pharmacopoeia), and "al-Qānūn fīl-tibb” (The Canon of Medicine) were completely studied. It was found that both nutritional factors such as glucose and electrolytes and non-nutritional factors including female tract, sperm, age, the timing of sexual intercourse, maternal body mass, stress, pH, estrogen, temperature, and testosterone can be used as important indicators in the gender selection. Most recommendations provided by PM for gender selection were found to be in good agreement with those reported by modern medicine.
性别选择在不同的国家已成为一个有争议的问题，在这方面，广泛的研究和国际专题讨论会都致力于这一主题。在不限制出版日期的情况下进行了全面的文献综述。此外，可靠的波斯医学(PM)资源，如“Exir-e-Azam”(伟大的长生不老药)，“Qarabadin-e-Kabir”(伟大的药典)和“al-Qānūn f l-tibb”(医学经典)进行了全面研究。研究发现，营养因素(如葡萄糖、电解质)和非营养因素(如女性生殖道、精子、年龄、性交时间、产妇体重、应激、pH、雌激素、体温、睾酮等)都可以作为性别选择的重要指标。PM提供的大多数性别选择建议与现代医学报告的建议一致。
{"title":"Comparative Study on Gender Selection: Persian Medicine and Modern Medicine","authors":"Qodsiye Hosseinifakhr, Yasaman Vahedi-Mazdabadi, Mina Saeedi, M. S. Shams Ardekani","doi":"10.18502/tim.v8i2.13084","DOIUrl":"https://doi.org/10.18502/tim.v8i2.13084","url":null,"abstract":"Gender selection has emerged as a controversial issue in different countries and in this respect, a wide range of studies and international symposiums have been dedicated to this subject. A comprehensive literature review was performed without restriction on publication date. Also, the credible Persian Medicine (PM) resources such as \"Exir-e-Azam\" (The Great Elixir), \"Qarabadin-e-Kabir\" (Great Pharmacopoeia), and \"al-Qānūn fīl-tibb” (The Canon of Medicine) were completely studied. It was found that both nutritional factors such as glucose and electrolytes and non-nutritional factors including female tract, sperm, age, the timing of sexual intercourse, maternal body mass, stress, pH, estrogen, temperature, and testosterone can be used as important indicators in the gender selection. Most recommendations provided by PM for gender selection were found to be in good agreement with those reported by modern medicine.","PeriodicalId":52659,"journal":{"name":"Traditional and Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48269386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mohammad Sadegh Adel Mehraban, Zahra Aghabeiglooei, R. Atlasi, N. Namazi, M. Ayati
As a phytochemical, berberine can modulate metabolic parameters via altering gut flora. However, findings are conflicting. In the present systematic review, we aimed to summarize the effects of berberine on gut microbiota in the models of metabolic disorders in both animal studies and clinical trials. Publications in five electronic databases including PubMed, Scopus, Embase, Web of Science, and Cochrane Library were searched systematically up to 31 May 2021 to find relevant articles with English language. Out of 4102 studies (including 2125 duplicates), 35 studies were included. In animal studies, various effects of berberine on beneficial and harmful microbiota were reported. However, findings also indicated that berberine can decrease the Firmicutes to Bacteroidetes (F/B) ratio. Three out of five studies showed positive effects of berberine on the production of short-chain fatty acids (SCFA), particularly butyrate. In three animal studies, lipopolysacaride (LPS) concentrations decreased with berberine administration. In clinical trials (n=3) positive effects on microbiota and metabolic status were also reported. However, the quality of clinical trials was mainly low. The present systematic review showed that berberine can modulate key metabolic parameters through improving the balance of intestinal microbiome, decreasing the abundance of harmful microbiota and LPS concentrations, and increasing the production of SCFAs, particularly butyrate in animal models. However, there are limited high-quality evidence regarding the effects of berberine on gut flora in clinical trials. Although berberine can be an effective prebiotic supplement to modulate metabolic parameters, further high-quality clinical trials are needed to confirm this potential.
作为一种植物化学物质，黄连素可以通过改变肠道菌群来调节代谢参数。然而，研究结果相互矛盾。在本系统综述中，我们旨在总结黄连素在动物研究和临床试验中对代谢紊乱模型中肠道微生物群的影响。截至2021年5月31日，系统搜索了PubMed、Scopus、Embase、Web of Science和Cochrane Library等五个电子数据库中的出版物，以查找英语相关文章。在4102项研究（包括2125项重复）中，纳入了35项研究。在动物研究中，报道了黄连素对有益和有害微生物群的各种影响。然而，研究结果也表明，黄连素可以降低厚壁菌门与拟杆菌门（F/B）的比例。五分之三的研究表明，黄连素对短链脂肪酸（SCFA）的产生，特别是丁酸盐的产生有积极作用。在三项动物研究中，脂多糖（LPS）浓度随着黄连素的给药而降低。在临床试验中（n=3）也报告了对微生物群和代谢状态的积极影响。然而，临床试验的质量主要较低。目前的系统综述表明，在动物模型中，黄连素可以通过改善肠道微生物组的平衡、降低有害微生物群的丰度和LPS浓度以及增加SCFAs的产生，特别是丁酸盐的产生，来调节关键的代谢参数。然而，临床试验中关于黄连素对肠道菌群影响的高质量证据有限。尽管黄连素可以作为一种有效的益生元补充剂来调节代谢参数，但还需要进一步的高质量临床试验来证实这一潜力。
{"title":"Berberine as a Natural Modifier of Gut Microbiota to Promote Metabolic Status in Animal Studies and Clinical Trials: A Systematic Review","authors":"Mohammad Sadegh Adel Mehraban, Zahra Aghabeiglooei, R. Atlasi, N. Namazi, M. Ayati","doi":"10.18502/tim.v8i2.13086","DOIUrl":"https://doi.org/10.18502/tim.v8i2.13086","url":null,"abstract":"As a phytochemical, berberine can modulate metabolic parameters via altering gut flora. However, findings are conflicting. In the present systematic review, we aimed to summarize the effects of berberine on gut microbiota in the models of metabolic disorders in both animal studies and clinical trials. Publications in five electronic databases including PubMed, Scopus, Embase, Web of Science, and Cochrane Library were searched systematically up to 31 May 2021 to find relevant articles with English language. Out of 4102 studies (including 2125 duplicates), 35 studies were included. In animal studies, various effects of berberine on beneficial and harmful microbiota were reported. However, findings also indicated that berberine can decrease the Firmicutes to Bacteroidetes (F/B) ratio. Three out of five studies showed positive effects of berberine on the production of short-chain fatty acids (SCFA), particularly butyrate. In three animal studies, lipopolysacaride (LPS) concentrations decreased with berberine administration. In clinical trials (n=3) positive effects on microbiota and metabolic status were also reported. However, the quality of clinical trials was mainly low. The present systematic review showed that berberine can modulate key metabolic parameters through improving the balance of intestinal microbiome, decreasing the abundance of harmful microbiota and LPS concentrations, and increasing the production of SCFAs, particularly butyrate in animal models. However, there are limited high-quality evidence regarding the effects of berberine on gut flora in clinical trials. Although berberine can be an effective prebiotic supplement to modulate metabolic parameters, further high-quality clinical trials are needed to confirm this potential.","PeriodicalId":52659,"journal":{"name":"Traditional and Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48296610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In humans, the immune system serves as a protective barrier against infection; however, when the immune system is out of balance, it can harm the host. Immunomodulators are chemicals or medications that have been employed in the clinic to treat an unbalanced immune response. The majority of immunological medicines in clinical use are cytotoxic. They harm the patient's quality of life by causing various side effects and being associated with higher production costs, longer lead times, and a high failure rate. Furthermore, obtaining single-compound chemicals with low toxicity, high efficacy, and selectivity for specified disorders is difficult for researchers. As a result, techniques based on alternative medicine are gaining attraction in drug development, focusing on innovative natural compounds utilized to treat various disorders. Many plant molecules founded to have biologically beneficial properties. This review aimed to look at the immunomodulatory activity of plant-derived chemicals from widely-used plants.
{"title":"A Review on Plant-Derived Immunomodulatory Agents: Hopes as an Alternative Medicine in the Management of Immune-Related Disorders","authors":"K. Deva, B. Bose, D. Basavan","doi":"10.18502/tim.v8i2.13085","DOIUrl":"https://doi.org/10.18502/tim.v8i2.13085","url":null,"abstract":"In humans, the immune system serves as a protective barrier against infection; however, when the immune system is out of balance, it can harm the host. Immunomodulators are chemicals or medications that have been employed in the clinic to treat an unbalanced immune response. The majority of immunological medicines in clinical use are cytotoxic. They harm the patient's quality of life by causing various side effects and being associated with higher production costs, longer lead times, and a high failure rate. Furthermore, obtaining single-compound chemicals with low toxicity, high efficacy, and selectivity for specified disorders is difficult for researchers. As a result, techniques based on alternative medicine are gaining attraction in drug development, focusing on innovative natural compounds utilized to treat various disorders. Many plant molecules founded to have biologically beneficial properties. This review aimed to look at the immunomodulatory activity of plant-derived chemicals from widely-used plants. \u0000 ","PeriodicalId":52659,"journal":{"name":"Traditional and Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45154358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hanieh Yadollahi, Fahimeh Safaeinejad, H. Hajimehdipoor, Z. Mousavi, L. Ara, S. Esmaeili
Fatigue is a complex phenomenon that is explained as difficulty starting or keeping voluntary physical or mental activity leading to negative impacts on life and work performance. This study aimed to investigate the anti-fatigue effects of Viola odorata L. in an animal model. Aqueous and ethanol extracts of V. odorata were prepared and total phenolics content was determined. Then, the anti-fatigue activity of the extracts was evaluated via a weight-loaded forced swimming test in the rat. To this end, 48 male Wistar rats were randomly classified into 6 groups. The control group using distilled water and other groups with ethanol (EVO; 50, 100, 200 mg/kg) and aqueous extracts of V. odorata (WVO; 50, 100mg/kg) were gavaged once daily for four weeks. Then, the forced swimming was conducted and swimming time, as a fatigue factor was measured. In addition, to validate the effect of V. odorata on the endurance capacity of the rats, biochemical factors including glucose, lactate dehydrogenase (LDH), and tumor necrosis factor (TNF-α) were examined in the serum. Hepatotoxicity was also assessed using hematoxylin-eosin staining. Our data indicated that the forced swimming time of the EVO-100, EVO-200, and WVO-100 groups was significantly increased. The serum glucose in the group which received EVO-200 was increased significantly; while serum LDH levels in all treated groups were significantly decreased. Also, the serum level of TNF-α in the groups which received EVO-100 or 200 was increased significantly. However, there was no considerable difference in serum TNF-α level and no hepatotoxicity within aqueous extract groups. Pathology results showed fewer effects of the aqueous extract rather than ethanol extract on the liver. The results provide evidence for the development and use of V. odorata aqueous extract as an anti-fatigue supplement.
{"title":"Anti-Fatigue Effect of Viola odorata L. in Forced Swimming Test in Rat","authors":"Hanieh Yadollahi, Fahimeh Safaeinejad, H. Hajimehdipoor, Z. Mousavi, L. Ara, S. Esmaeili","doi":"10.18502/tim.v8i2.13083","DOIUrl":"https://doi.org/10.18502/tim.v8i2.13083","url":null,"abstract":"Fatigue is a complex phenomenon that is explained as difficulty starting or keeping voluntary physical or mental activity leading to negative impacts on life and work performance. This study aimed to investigate the anti-fatigue effects of Viola odorata L. in an animal model. Aqueous and ethanol extracts of V. odorata were prepared and total phenolics content was determined. Then, the anti-fatigue activity of the extracts was evaluated via a weight-loaded forced swimming test in the rat. To this end, 48 male Wistar rats were randomly classified into 6 groups. The control group using distilled water and other groups with ethanol (EVO; 50, 100, 200 mg/kg) and aqueous extracts of V. odorata (WVO; 50, 100mg/kg) were gavaged once daily for four weeks. Then, the forced swimming was conducted and swimming time, as a fatigue factor was measured. In addition, to validate the effect of V. odorata on the endurance capacity of the rats, biochemical factors including glucose, lactate dehydrogenase (LDH), and tumor necrosis factor (TNF-α) were examined in the serum. Hepatotoxicity was also assessed using hematoxylin-eosin staining. Our data indicated that the forced swimming time of the EVO-100, EVO-200, and WVO-100 groups was significantly increased. The serum glucose in the group which received EVO-200 was increased significantly; while serum LDH levels in all treated groups were significantly decreased. Also, the serum level of TNF-α in the groups which received EVO-100 or 200 was increased significantly. However, there was no considerable difference in serum TNF-α level and no hepatotoxicity within aqueous extract groups. Pathology results showed fewer effects of the aqueous extract rather than ethanol extract on the liver. The results provide evidence for the development and use of V. odorata aqueous extract as an anti-fatigue supplement.","PeriodicalId":52659,"journal":{"name":"Traditional and Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49631776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. P. Hamedani, F. Aram, A. Hadjiakhoondi, N. Samadi, S. Tavakoli, F. Kiashi, S. Goodarzi, Z. Tofighi
The Stachys genus is a medicinal plant, with 34 different species, from Lamiaceae family that grows in Irano-Anatolian plateau. There are 12 species of Stachys native to Iran. Some species of Stachys have been used in traditional medicine of Iran, Turkey, Italy, Greece, China, and Japan for the treatment of various diseases like inflammation and common cold. Some researchers have noted many biological effects, such as cytotoxic, antioxidant, immunomodulator, anti-inflammatory and antibacterial effects for the members of this genus. There are a few evidences on phytochemical constituents of S. byzanthina. The aim of this study was phytochemical investigation of various extracts of this plant to obtain the most efficient fractions and compound(s) to study the antimicrobial effects of this plant. Separation and isolation of compounds was performed by various methods of chromatography. The structure of each compound was identified by spectroscopic methods. Antimicrobial studies were performed on different fractions of S. byzantina by microdilution method and to determine the Minimum Inhibitory Concentration (MIC), agar well diffusion was done. Ethyl acetate fraction has the highest total phenol contents (1082.05 mg GAE/g fraction) and antioxidant capacity (IC50= 1.23 mg/mL). Also, ethyl acetate fraction, the most potential fraction of S. byzanthina, demonstrated the largest inhibition zone (17.5 ± 0.7 mm) with 5 mg/mL in MIC against Staphylococcus auresus. Apigenin and its derivatives were isolated from this fraction and according to literature; they can be responsible for antibacterial activity of this fraction of S. byzanthina.
{"title":"Elucidation of Apigenin Derivatives from Ethyl Acetate Fraction of Stachys byzantina with Potent Antioxidant and Anti-Staphylococcus aureus Properties","authors":"M. P. Hamedani, F. Aram, A. Hadjiakhoondi, N. Samadi, S. Tavakoli, F. Kiashi, S. Goodarzi, Z. Tofighi","doi":"10.18502/tim.v8i2.13082","DOIUrl":"https://doi.org/10.18502/tim.v8i2.13082","url":null,"abstract":"The Stachys genus is a medicinal plant, with 34 different species, from Lamiaceae family that grows in Irano-Anatolian plateau. There are 12 species of Stachys native to Iran. Some species of Stachys have been used in traditional medicine of Iran, Turkey, Italy, Greece, China, and Japan for the treatment of various diseases like inflammation and common cold. Some researchers have noted many biological effects, such as cytotoxic, antioxidant, immunomodulator, anti-inflammatory and antibacterial effects for the members of this genus. There are a few evidences on phytochemical constituents of S. byzanthina. The aim of this study was phytochemical investigation of various extracts of this plant to obtain the most efficient fractions and compound(s) to study the antimicrobial effects of this plant. Separation and isolation of compounds was performed by various methods of chromatography. The structure of each compound was identified by spectroscopic methods. Antimicrobial studies were performed on different fractions of S. byzantina by microdilution method and to determine the Minimum Inhibitory Concentration (MIC), agar well diffusion was done. Ethyl acetate fraction has the highest total phenol contents (1082.05 mg GAE/g fraction) and antioxidant capacity (IC50= 1.23 mg/mL). Also, ethyl acetate fraction, the most potential fraction of S. byzanthina, demonstrated the largest inhibition zone (17.5 ± 0.7 mm) with 5 mg/mL in MIC against Staphylococcus auresus. Apigenin and its derivatives were isolated from this fraction and according to literature; they can be responsible for antibacterial activity of this fraction of S. byzanthina.","PeriodicalId":52659,"journal":{"name":"Traditional and Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49640186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A previous study has reported the interesting relaxant effect of the hydroalcoholic extract from Waltheria indica L. (Malvaceae) leafy stems, a plant with several therapeutic uses. The present study aimed to investigate the preventive effect of this plant using an ex vivo model of the rat trachea. Two agonists, acetylcholine (10-6 - 1.5×10-5 M) and barium chloride (10-5- 10-1 M) were used to induce contractions. The preventive effect was assessed on rat tracheal rings pretreated with hydroalcoholic extract (1.92 mg/mL), glibenclamide, atropine, and papaverine, all at 10 µM. Acetylcholine and barium chloride provoke contractions in a concentration-dependent manner, with a maximum contractile response of 3.953 ± 0.692 g and 2.999 ± 0.326 g, respectively. The EC50 values were 3.711 ± 0.823 µM and 9.502 ± 12.354 mM, respectively, for acetylcholine- and barium chloride-induced contraction. Glibenclamide caused a rightward shift of the acetylcholine-response curve, followed by a reduction of the maximum contraction (from 3.953 ± 0.692 g to 3. 116 ± 0.244 g). The hydroalcoholic extract, atropine, papaverine, and their combinations induced a complete suppression of the contractile response to acetylcholine (p < 0.0001) and barium chloride (p < 0.0001). The hydroalcoholic extract exhibited a potent relaxant effect comparable to that of atropine and papaverine. It can be concluded that the hydroalcoholic extract of W. indica can potently prevent acetylcholine-and barium chloride-induced contractions. The possible mechanisms by which the extract exerts its relaxant effect may involve the blockade of muscarinic receptors, the inhibition of phosphodiesterase activity, and/or the calcium channel.
{"title":"Effect of the Hydroalcoholic Extract from the Leafy Stems of Waltheria indica L. (Malvaceae) on Acetylcholine and Barium Chloride-Induced Contractions on Isolated Rat Tracheal Tissue","authors":"Yabré Zakaline, Boly Rainatou, Nitiéma Mathieu, Belemnaba Lazare, Youl Noëla Hoho Estelle, Guissou Innocent Pierre, Ouédraogo Sylvin","doi":"10.18502/tim.v8i1.12402","DOIUrl":"https://doi.org/10.18502/tim.v8i1.12402","url":null,"abstract":"A previous study has reported the interesting relaxant effect of the hydroalcoholic extract from Waltheria indica L. (Malvaceae) leafy stems, a plant with several therapeutic uses. The present study aimed to investigate the preventive effect of this plant using an ex vivo model of the rat trachea. Two agonists, acetylcholine (10-6 - 1.5×10-5 M) and barium chloride (10-5- 10-1 M) were used to induce contractions. The preventive effect was assessed on rat tracheal rings pretreated with hydroalcoholic extract (1.92 mg/mL), glibenclamide, atropine, and papaverine, all at 10 µM. Acetylcholine and barium chloride provoke contractions in a concentration-dependent manner, with a maximum contractile response of 3.953 ± 0.692 g and 2.999 ± 0.326 g, respectively. The EC50 values were 3.711 ± 0.823 µM and 9.502 ± 12.354 mM, respectively, for acetylcholine- and barium chloride-induced contraction. Glibenclamide caused a rightward shift of the acetylcholine-response curve, followed by a reduction of the maximum contraction (from 3.953 ± 0.692 g to 3. 116 ± 0.244 g). The hydroalcoholic extract, atropine, papaverine, and their combinations induced a complete suppression of the contractile response to acetylcholine (p < 0.0001) and barium chloride (p < 0.0001). The hydroalcoholic extract exhibited a potent relaxant effect comparable to that of atropine and papaverine. It can be concluded that the hydroalcoholic extract of W. indica can potently prevent acetylcholine-and barium chloride-induced contractions. The possible mechanisms by which the extract exerts its relaxant effect may involve the blockade of muscarinic receptors, the inhibition of phosphodiesterase activity, and/or the calcium channel.","PeriodicalId":52659,"journal":{"name":"Traditional and Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45223819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Monsef-Esfahani, Sahand Jamshidi, P. Sharafi-Badr, M. Sharifzadeh, M. Vazirian, S. N. Sadati Lamardi
This study aimed to assess the antinociceptive activity of extracts and fractions of Paeonia daurica subsp. macrophylla in BALB/c mice. Various doses of hydro-alcoholic extract (HE), hexane fraction (F-hexane), methanol (F-MeOH), and chloroform (F-CHCl3), as well as aqueous extracts (AE), were evaluated by a well-known model, a formalin-induced pain test in mice. All extracts, piroxicam 0.1 mg/kg, and negative control groups were administered 30 minutes before formalin injection. Flinching, licking, and biting reflexes were measured as painful factors compared with controls at intervals of 0 to 5 minutes, 0 to 15, and 0 to 60 minutes after formalin injection. The acute oral toxicity test of total ethanolic and aqueous extracts showed no signs of toxic effect up to a dose of 5000 mg/kg. In the formalin test at a time interval of 0 to 5 minutes, there was no significant difference between the results of the study groups. In the range of 0 to 15 minutes, the effect of AE (1 g/kg), HE (2, 3 g/kg), and F-hexane (1 g/kg) was significantly higher than the positive control group (p<0.01). In the time interval of 0 to 60 minutes as the total time of the experiment, the effect of AE (0.25 g/kg), AE (0.5, 1 g/kg), HE (2, 3 g/kg), F-hexane (1 g/kg) were significantly different than the positive control group. It can be concluded that extract of P. daurica subsp. macrophylla might be helpful in the treatment of pain in humans.
{"title":"Antinociceptive Effects of Paeonia daurica subsp. macrophylla Root Extracts in Mice","authors":"H. Monsef-Esfahani, Sahand Jamshidi, P. Sharafi-Badr, M. Sharifzadeh, M. Vazirian, S. N. Sadati Lamardi","doi":"10.18502/tim.v8i1.12398","DOIUrl":"https://doi.org/10.18502/tim.v8i1.12398","url":null,"abstract":"This study aimed to assess the antinociceptive activity of extracts and fractions of Paeonia daurica subsp. macrophylla in BALB/c mice. Various doses of hydro-alcoholic extract (HE), hexane fraction (F-hexane), methanol (F-MeOH), and chloroform (F-CHCl3), as well as aqueous extracts (AE), were evaluated by a well-known model, a formalin-induced pain test in mice. All extracts, piroxicam 0.1 mg/kg, and negative control groups were administered 30 minutes before formalin injection. Flinching, licking, and biting reflexes were measured as painful factors compared with controls at intervals of 0 to 5 minutes, 0 to 15, and 0 to 60 minutes after formalin injection. The acute oral toxicity test of total ethanolic and aqueous extracts showed no signs of toxic effect up to a dose of 5000 mg/kg. In the formalin test at a time interval of 0 to 5 minutes, there was no significant difference between the results of the study groups. In the range of 0 to 15 minutes, the effect of AE (1 g/kg), HE (2, 3 g/kg), and F-hexane (1 g/kg) was significantly higher than the positive control group (p<0.01). In the time interval of 0 to 60 minutes as the total time of the experiment, the effect of AE (0.25 g/kg), AE (0.5, 1 g/kg), HE (2, 3 g/kg), F-hexane (1 g/kg) were significantly different than the positive control group. It can be concluded that extract of P. daurica subsp. macrophylla might be helpful in the treatment of pain in humans.","PeriodicalId":52659,"journal":{"name":"Traditional and Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42937157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Medicinal and aromatic plants have been one of the most important sources of medicine since the dawn of human civilization. Indigenous communities have used products from these plants in different conditions throughout history. Cannabis sativa L. is one of the most widely employed herbaceous medicinal plants for textiles, and fibers, in medicine, as a source of food, animal food, animal bedding, and agriculture for seeds. This paper highlights the traditional applications, botany, phytochemistry, and pharmacological properties of C. sativa. Extensive database retrieval, such as Google Scholar, Semantic Scholar, ResearchGate, Academia.edu, PubMed, SciFinder, ChemSpider, CNKI, PubFacts was performed using the keywords “Hemp” and “Cannabis,” as well as the scientific name of this plant species (Cannabis sativa). Besides, reviews of relevant textbooks, documents, and patents were also employed to collect sufficient information. This study revealed numerous pharmacological activities of C. sativa that could help with several health issues. Additionally, more than 565 bioactive constituents have been isolated and identified from diverse parts of C. sativa. This could help discover potential therapeutic effects and develop new medications to benefit human health.
{"title":"Cannabis sativa L.: A Review on Traditional Uses, Botany, Phytochemistry, and Pharmacological Aspects","authors":"Noureddine Chaachouaya, Abdelhamid Azerouala, Bouchaib Bencharkia, Allal Douirab, Lahcen Zidaneb","doi":"10.18502/tim.v8i1.12407","DOIUrl":"https://doi.org/10.18502/tim.v8i1.12407","url":null,"abstract":"Medicinal and aromatic plants have been one of the most important sources of medicine since the dawn of human civilization. Indigenous communities have used products from these plants in different conditions throughout history. Cannabis sativa L. is one of the most widely employed herbaceous medicinal plants for textiles, and fibers, in medicine, as a source of food, animal food, animal bedding, and agriculture for seeds. This paper highlights the traditional applications, botany, phytochemistry, and pharmacological properties of C. sativa. Extensive database retrieval, such as Google Scholar, Semantic Scholar, ResearchGate, Academia.edu, PubMed, SciFinder, ChemSpider, CNKI, PubFacts was performed using the keywords “Hemp” and “Cannabis,” as well as the scientific name of this plant species (Cannabis sativa). Besides, reviews of relevant textbooks, documents, and patents were also employed to collect sufficient information. This study revealed numerous pharmacological activities of C. sativa that could help with several health issues. Additionally, more than 565 bioactive constituents have been isolated and identified from diverse parts of C. sativa. This could help discover potential therapeutic effects and develop new medications to benefit human health.","PeriodicalId":52659,"journal":{"name":"Traditional and Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46775658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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