Current Research in Biotechnology最新文献_第5页

Curcumol inhibits hepatocellular carcinoma proliferation through miRNA-124/STAT3 pathway: Network pharmacology and experimental validation 莪术醇通过miRNA-124/STAT3通路抑制肝癌细胞增殖：网络药理学及实验验证

IF 3.6 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Current Research in Biotechnology

Pub Date : 2025-01-01 DOI: 10.1016/j.crbiot.2024.100270

Gui-yu Li , Ji-yong Lin

Objective

Hepatocellular carcinoma (HCC) is the most common type of liver cancer and is one of the most common global cancers. Curcuma zedoaria (Christm.) Roscoe is a traditional Chinese herb that has been used for thousands of years in China to treat various types of cancer. Curcumol is one of its primary bioactive sesquiterpenes and has been reported to possess antitumor properties; however, the underlying mechanisms in hepatocellular carcinoma (HCC) are largely unknown. The aim of this study was to reveal the mechanism of curcumol treating HCC based on the network pharmacology and experimental verification.

Materials and Methods

Targets of HCC and curcumol were identified. The drugs and disease targets were intersected by Venn Diagram. The KEGG pathway enrichment analysis of curcumol treating HCC was analyzed through the R 3.6.1 software. The effects of curcumol on the inhibition of HCC cell line HepG2 growth and its pro-apoptotic activity were evaluated by cell counting kit-8 and flow cytometry. The expression of microRNA-124 (miRNA-124) mRNA was detected by quantitative real-time PCR. HepG2 cells were transfected with a miRNA mimic and inhibitor. The expression of STAT3 and its phosphorylation were induced by IL-6 and detected by western blotting.

Results

MicroRNAs in cancer is a significant enrichment signaling pathway for curcumol treating HCC, according to the KEGG pathway analysis. Curcumol effectively inhibited HepG2 cell growth at 50–150 μg/ml, while it had low toxicity to normal LO2 cells. Using flow cytometry, curcumol strongly promoted apoptosis in HepG2 cells and was more potent than the miRNA-124 mimic, whereas the miRNA-124 inhibitor reduced the pro-apoptotic effect of curcumol. Western blotting revealed that curcumol significantly downregulated the overexpression of STAT3 and its phosphorylation in interleukin-6 induced HepG2 cells, whereas an increased level of STAT3 was observed in the miRNA-124 inhibitor transfected cells after curcumol treatment compared to untransfected cells. The level of miRNA-124 was changed up to 5.87-fold by curcumol treatment.

Conclusions

The mechanism underlying the effect of curcumol on inhibition and pro-apoptosis of HepG2 cell growth is possibly related to the miRNA-124/STAT3 pathway.

目的肝细胞癌（HCC）是最常见的肝癌类型，也是全球最常见的癌症之一。莪术（圣诞节）罗斯科是一种传统的中国草药，在中国已经使用了数千年，用于治疗各种类型的癌症。姜黄酚是其主要生物活性倍半萜之一，据报道具有抗肿瘤特性；然而，肝细胞癌（HCC）的潜在机制在很大程度上是未知的。本研究旨在通过网络药理学和实验验证，揭示姜黄酚治疗肝癌的机制。材料与方法确定肝癌和姜黄酚的治疗靶点。通过维恩图将药物与疾病靶点相交。通过r3.6.1软件分析姜黄酚治疗HCC的KEGG通路富集分析。采用细胞计数试剂盒-8和流式细胞术观察莪术酚对肝癌细胞株HepG2生长的抑制作用及其促凋亡活性。采用实时荧光定量PCR检测microRNA-124 (miRNA-124) mRNA的表达。用miRNA模拟物和抑制剂转染HepG2细胞。IL-6诱导STAT3表达及磷酸化，western blotting检测。结果根据KEGG通路分析，肿瘤中的microrna是姜黄酚治疗HCC的重要富集信号通路。莪术酚在50 ~ 150 μg/ml浓度下能有效抑制HepG2细胞的生长，对正常LO2细胞的毒性较低。流式细胞术发现，姜黄酚能显著促进HepG2细胞凋亡，且比miRNA-124模拟物更有效，而miRNA-124抑制剂则降低了姜黄酚的促凋亡作用。Western blotting结果显示，姜黄酚显著下调白细胞介素-6诱导的HepG2细胞中STAT3的过表达及其磷酸化，而与未转染的细胞相比，姜黄酚处理后转染miRNA-124抑制剂的细胞中STAT3的水平升高。经姜黄酚处理后，miRNA-124的表达水平变化达5.87倍。结论姜黄酚抑制HepG2细胞生长和促凋亡的机制可能与miRNA-124/STAT3通路有关。

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引用次数: 0

Corrigendum to “Cost-effective production process of scFv antibody fragments against Shiga toxin 2 via recombinant E. coli” [Curr. Res. Biotechnol. 10 (2025) 100310] “通过重组大肠杆菌生产抗志贺毒素2的单链病毒抗体片段的成本效益方法”的勘误[c]。参考文献。生物技术。10 (2025)100310 [j]

IF 4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Current Research in Biotechnology

Pub Date : 2025-01-01 DOI: 10.1016/j.crbiot.2025.100315

Marcela Guimarães , Daniela Luz , Elisabeth de Fátima Pires Augusto , Lucia Vieira , Maricilia Silva Costa , Roxane Maria Fontes Piazza , José Geraldo da Cruz Pradella

引用次数: 0

Edible domestic animal blood products for biomedical and disease treatment applications 用于生物医学和疾病治疗的食用家畜血液制品

IF 3.6 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Current Research in Biotechnology

Pub Date : 2025-01-01 DOI: 10.1016/j.crbiot.2025.100308

Zhi Cao , Imran Mahmood Khan , Xiangrui Niu , Yang Hu , Zhuang Duan , Qiang Fang , Zhouping Wang , Junsong Yang

Edible blood products from domestic livestock are high-value derivatives produced during slaughter, with significant potential in biomedicine and disease treatment. Livestock blood is rich in proteins, peptides, iron, vitamins and other bioactive substances. Among them, functional proteins and peptides have been widely studied for their prominent roles in drug delivery, immune regulation, anti-infection and antioxidation. They significantly impact cancer treatment and immune regulation, providing valuable resources for innovation in the food and pharmaceutical industries. The development and utilization of livestock blood products offer innovative solutions for disease treatment and promote the sustainable development of the food and pharmaceutical industries by reducing the environmental burden of blood waste. However, challenges such as the complexity of blood composition, the variability of bioactive compound effectiveness, and the need for advanced purification techniques limit their broader applications. Additionally, regulatory and safety concerns must be addressed for therapeutic suitability. This review highlights the recent advancements in the biomedical applications of livestock blood products in biomedicine, addressing these challenges and emphasizing their potential to reduce environmental waste and promote sustainable development. This review consolidates current knowledge to provide a foundation for future research, fostering innovation in disease intervention and therapy while addressing utilization barriers.

家畜食用血制品是屠宰过程中产生的高价值衍生物，在生物医学和疾病治疗方面具有巨大潜力。家畜血液中含有丰富的蛋白质、多肽、铁、维生素等生物活性物质。其中功能蛋白和肽因其在药物传递、免疫调节、抗感染和抗氧化等方面的突出作用而受到广泛研究。它们显著影响癌症治疗和免疫调节，为食品和制药行业的创新提供宝贵的资源。牲畜血液制品的开发和利用为疾病治疗提供了创新的解决方案，并通过减少血液废物的环境负担来促进食品和制药行业的可持续发展。然而，诸如血液成分的复杂性、生物活性化合物有效性的可变性以及对先进纯化技术的需求等挑战限制了它们的广泛应用。此外，必须解决治疗适宜性的监管和安全问题。本文综述了家畜血液制品在生物医学中的生物医学应用的最新进展，解决了这些挑战，并强调了它们在减少环境浪费和促进可持续发展方面的潜力。这篇综述巩固了现有的知识，为未来的研究提供了基础，促进了疾病干预和治疗的创新，同时解决了利用障碍。

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引用次数: 0

A comparative study of the cis and trans isomers of two hydantoin compounds with anticonvulsant potency 两种具有抗惊厥作用的氢妥英化合物的顺式和反式异构体的比较研究

IF 3.6 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Current Research in Biotechnology

Pub Date : 2025-01-01 DOI: 10.1016/j.crbiot.2025.100317

Jana Tchekalarova , Tsveta Stoyanova , Petar Todorov

Hydantoins are a class of compounds renowned for their diverse medical applications, particularly in neurology. Phenytoin (Dilantin) is one of the most well-known hydantoins, primarily used to stabilize neuronal membranes and prevent the spread of seizure activity. Recently, our team synthesized and studied the physicochemical properties of two compounds, 3-amino-5,5′-dimethylhydantoin Schiff Bases (SB1 and SB2). We hypothesized that SB1 and SB2 possess anticonvulsant properties, with the cis forms expected to be more active than their trans forms. We conducted experiments using the maximal electroshock (MES) test and corneal kindling in mice to test this hypothesis. The results showed that the cis isomers of SB1 and SB2 exhibited more potent activity against seizure spread than their trans isomers, and this was observed at doses that did not induce a sedative effect. Furthermore, both trans and cis isomers of SB1 but not SB2 compound demonstrated the ability to suppress seizures in the corneal kindling test. Our findings suggest that the trans-cis conversion of the hydantoin compounds SB1 and SB2 is crucial for their effectiveness in preventing seizure spread in the MES test and that of SB1 in the corneal kindling mouse model.

海托因是一类以其多种医学应用而闻名的化合物，特别是在神经病学方面。苯妥英（苯妥英）是最著名的苯妥英之一，主要用于稳定神经元膜和防止癫痫活动的扩散。最近，我们的团队合成并研究了两种化合物，3-氨基-5,5 ' -二甲基海因希夫碱（SB1和SB2）的理化性质。我们假设SB1和SB2具有抗惊厥的特性，顺式比反式更有活性。我们在小鼠身上进行了最大电休克（MES）试验和角膜点燃实验来验证这一假设。结果表明，SB1和SB2的顺式异构体比其反式异构体表现出更强的抗癫痫扩散活性，这是在不诱导镇静作用的剂量下观察到的。此外，SB1的反式和顺式异构体在角膜点燃试验中均表现出抑制癫痫发作的能力，而SB2化合物则没有。我们的研究结果表明，海因化合物SB1和SB2的跨式转化对其在MES试验和角膜点燃小鼠模型中预防癫痫扩散的有效性至关重要。

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引用次数: 0

Health-promoting and medicinal properties of Zingiberaceae family plants: A minireview with a special focus on galangal, turmeric, cardamom, and ginger 姜科植物对健康的促进作用和药用特性：以高良姜、姜黄、小豆蔻和姜为重点的综述

IF 4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Current Research in Biotechnology

Pub Date : 2025-01-01 DOI: 10.1016/j.crbiot.2025.100329

Maima Matin , Rajeev K. Singla , Artur Jóźwik , Jarosław Olav Horbańczuk , Natalia Ksepka , Kamil Wysocki , Thadiyan Parambil Ijinu , Neenthamadathil Mohandas Krishnakumar , Sreejith Pongillyathundiyil Sasidharan , Ifeoma C. Ezenyi , John Igoli , Fabio Fusi , Sara Frazzini , Luciana Rossi , Michel-Edwar Mickael , Abhishek Joshi , Olga Adamska , Artur Stolarczyk , Esra Capanoglu , Deniz Gunal-Koroglu , Atanas G. Atanasov

The Zingiberaceae family, including among others, galangal (Alpinia galanga), turmeric (Curcuma longa), cardamom (Elettaria cardamomum), and ginger (Zingiber officinale), has been widely used in traditional medicine and culinary practices worldwide due to its diverse health-promoting properties. This mini-review aims to provide a concise overview of Zingiberaceae species’ medicinal potential and identify key areas for further research to facilitate their integration into modern medicine. Herein we summarize the existing research on the pharmacological activities of these species, with a focus on their antioxidant, anti-inflammatory, antimicrobial, anticancer, cardiovascular, digestive, and metabolic effects. Aside from the reported biological effects of traditional formulations and phytopharmaceutical preparations, emphasis is given to the primary bioactive compounds identified in these plants including diverse phenolics, terpenes, and various other secondary metabolites. Mechanisms contributing to therapeutic benefits. Moreover, highlighted is the promise of these plants for future development of drugs and nutraceuticals despite current challenges, particularly bioavailability issues and the need for more clinical studies.

姜科植物，包括高良姜（Alpinia galanga）、姜黄（Curcuma longa）、小豆蔻（Elettaria cardamomum）和生姜（Zingiber officinale），由于其多种促进健康的特性，在世界范围内广泛用于传统医学和烹饪实践。本文旨在简要介绍姜科植物的药用潜力，并确定进一步研究的重点领域，以促进其融入现代医学。本文对这些植物的药理作用进行了综述，重点介绍了它们的抗氧化、抗炎、抗菌、抗癌、心血管、消化和代谢作用。除了报道的传统配方和植物药物制剂的生物效应外，重点是在这些植物中鉴定的主要生物活性化合物，包括各种酚类物质，萜烯和各种其他次生代谢物。促进治疗益处的机制。此外，尽管目前面临挑战，特别是生物利用度问题和需要更多的临床研究，但这些植物对未来药物和保健品开发的前景是突出的。

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引用次数: 0

The immunomodulatory effects of chito-oligosaccharides derived from Alcaligenes faecalis chitinase on cyclophosphamide-induced immunosuppression in mice 粪碱性几丁质酶壳寡糖对环磷酰胺诱导小鼠免疫抑制的免疫调节作用

IF 4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Current Research in Biotechnology

Pub Date : 2025-01-01 DOI: 10.1016/j.crbiot.2025.100343

Rajesh K.M. , Anusha Govindula , Madhura M. Bose , Jayesh Mudgal , Ritu Raval

This study explores the immunomodulatory effects of chito-oligosaccharides (COS) derived from Alcaligenes faecalis chitinase in a cyclophosphamide-induced immunosuppression model in Swiss albino male mice. Mice received cyclophosphamide (80 mg/kg, i.p.) to induce immunosuppression, followed by oral administration of enzymatically derived COS (E-COS) and chemically synthesized COS (C-COS) at 20 mg/kg/day for 15 days. Both E-COS and C-COS significantly increased WBC (4.50 ± 0.71 and 5.37 ± 0.91) and RBC (5.80 ± 0.83 and 5.74 ± 0.86) counts compared to the cyclophosphamide group (1.43 ± 0.49 and 2.54 ± 0.76). Histopathological analysis showed thymic architecture restoration and reduced splenic lymphocyte apoptosis. Additionally, COS treatment elevated proinflammatory cytokine levels (TNF-α, IL-6, IL-1β) and decreased oxidative stress markers (malondialdehyde, glutathione). These findings suggest that COS possess immunomodulatory potential and may counteract cyclophosphamide-induced immunosuppression.

本研究探讨了粪Alcaligenes几丁质酶提取的壳寡糖（COS）在环磷酰胺诱导的瑞士白化雄性小鼠免疫抑制模型中的免疫调节作用。小鼠口服环磷酰胺（80 mg/kg, ig）诱导免疫抑制，然后分别口服酶促COS （E-COS）和化学合成COS (C-COS)，剂量为20 mg/kg/d，连续15 d。与环磷酰胺组（1.43±0.49和2.54±0.76）相比，E-COS和C-COS均显著增加WBC（4.50±0.71和5.37±0.91）和RBC（5.80±0.83和5.74±0.86）计数。组织病理学分析显示胸腺结构恢复，脾脏淋巴细胞凋亡减少。此外，COS处理可提高促炎细胞因子（TNF-α、IL-6、IL-1β）水平，降低氧化应激标志物（丙二醛、谷胱甘肽）。这些发现提示COS具有免疫调节潜能，可能抵消环磷酰胺诱导的免疫抑制。

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引用次数: 0

Remediation of cadmium-contaminated soil using Bacopa monnieri (L.) Wettst. Synergistic role of salicylic and jasmonic acids in Phytostabilisation and neuroprotective bacoside A biosynthesis 假马齿苋修复镉污染土壤的研究Wettst。水杨酸和茉莉酸在植物稳定和神经保护马尾草苷A生物合成中的协同作用

IF 4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Current Research in Biotechnology

Pub Date : 2025-01-01 DOI: 10.1016/j.crbiot.2025.100347

Abhishek Dadhich, Madan Mohan Sharma

Cadmium (Cd), a toxic trace element, poses a significant threat to plant physiology and secondary metabolite production, with implications for environmental and human health. Bacopa monnieri (BM), a medicinal plant valued for its neuroprotective properties, is highly sensitive to Cd, which reduces shoot biomass by approximately 50 % and chlorophyll content by approximately 30 %. Phytohormones, such as salicylic acid (SA) and jasmonic acid (JA), are recognized stress modulators; however, their combined influence on Cd detoxification and bacoside A production has not been reported. In this study, BM plants were exposed to Cd stress, with SA and JA applied individually and in combination. Cd-induced growth inhibition, pigment loss, and elevated root Cd accumulation were significantly alleviated by phytohormone treatments, with the SA + JA combination exhibiting the greatest effect. Co-treatment improved shoot and root biomass, restored chlorophyll levels, enhanced antioxidant enzyme activities, and reduced root Cd accumulation from 6340 to 3600 µg/g DW, thereby lowering the translocation factor from 0.16 to 0.10. Importantly, JA and SA + JA treatments increased bacoside A content by up to 1.7-fold, demonstrating enhanced secondary metabolite biosynthesis. This study provides the first experimental evidence that exogenous SA and JA synergistically mitigate Cd toxicity, strengthen phytostabilization, and boost bacoside A accumulation. These findings highlight the potential of BM as a dual-purpose candidate for phytoremediation and pharmaceutical exploitation in metal-contaminated ecosystems.

镉（Cd）是一种有毒的微量元素，对植物生理和次生代谢物的生产构成重大威胁，对环境和人类健康产生影响。假马齿苋（Bacopa monnieri， BM）是一种具有神经保护作用的药用植物，对Cd非常敏感，Cd使其茎部生物量减少约50%，叶绿素含量减少约30%。植物激素，如水杨酸（SA）和茉莉酸（JA）是公认的胁迫调节剂；然而，它们对Cd解毒和马齿苋苷A产生的综合影响尚未见报道。本研究以BM植株为研究对象，分别施用SA和JA和Cd胁迫。激素处理显著缓解了Cd诱导的生长抑制、色素损失和根系Cd积累升高，其中以SA + JA组合效果最好。共处理提高了茎部和根系生物量，恢复了叶绿素水平，增强了抗氧化酶活性，将根系Cd积累量从6340µg/g DW降低到3600µg/g DW，从而将转运因子从0.16降低到0.10。重要的是，JA和SA + JA处理使马齿苋苷A含量增加了1.7倍，表明次生代谢物的生物合成增强。本研究首次提供了外源SA和JA协同减轻Cd毒性、增强植物稳定性和促进马齿苋苷A积累的实验证据。这些发现突出了BM作为金属污染生态系统中植物修复和药物开发的双重候选物的潜力。

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引用次数: 0

The public discourse on genetics in autism communities: a content analysis of Reddit posts 自闭症群体中关于遗传学的公开讨论：对Reddit帖子的内容分析

IF 4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Current Research in Biotechnology

Pub Date : 2025-01-01 DOI: 10.1016/j.crbiot.2025.100346

Kamil R. Hiralal , Anneke Louwerse , Matea Skojo , Manon H.J. Hillegers , Hugo G. Schnack , Sabine E. Mous , Gwendolyn C. Dieleman

Purpose

There is ongoing debate in the autism community on the role of genetics. Most research examining the discourse on genetics in autism have focused primarily on caregivers. This study explored the public discourse on genetics within the broader autism community.

Methods

We collected 30,014 Reddit posts on genetics within autism communities, since the platform’s inception through August 1, 2024. Using topic modelling, we identified key topics and we conducted sentiment analysis to compare the emotional tone across these topics. Finally, we examined how user characteristics were related to the sentiment of a post.

Results

We identified seven topics related to genetics: personal stories, place in society, parental support, familial inheritance, healthcare, research, and moderator messages. Threads on healthcare and personal stories were relatively negative, whereas research and moderator messages threads were more positive. Comments on healthcare were relatively negative, while parental support and moderator messages comments were more positive. Users who generally post more positively also expressed more positive sentiment when discussing genetics in the context of autism.

Conclusions

The online discourse on genetics in online autism communities encompasses multiple topics. Researchers and clinicians should continuously engage with the autism community to address concerns regarding genetics in autism.

目的在自闭症群体中，关于基因的作用一直存在争论。大多数关于自闭症遗传学的研究主要集中在照顾者身上。这项研究探讨了在更广泛的自闭症群体中关于遗传学的公共话语。方法：从该平台成立到2024年8月1日，我们收集了30,014篇关于自闭症社区遗传学的Reddit帖子。通过主题建模，我们确定了关键主题，并进行了情绪分析，以比较这些主题的情绪基调。最后，我们研究了用户特征与帖子情绪的关系。结果：我们确定了与遗传学相关的七个主题：个人故事、社会地位、父母支持、家族遗传、医疗保健、研究和版主信息。关于医疗保健和个人故事的帖子相对消极，而研究和版主信息的帖子则更为积极。对医疗保健的评论相对消极，而父母支持和版主留言的评论则更为积极。在讨论自闭症背景下的遗传学时，通常发表更积极的帖子的用户也表达了更积极的情绪。在线自闭症社区中关于遗传学的在线讨论包含多个主题。研究人员和临床医生应该不断地与自闭症社区接触，以解决自闭症的遗传学问题。

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引用次数: 0

Role of network pharmacology integrated with experimental approaches in natural products drug discovery 网络药理学与实验方法在天然产物药物发现中的作用

IF 4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Current Research in Biotechnology

Pub Date : 2025-01-01 DOI: 10.1016/j.crbiot.2025.100338

Anoop Kumar, Devesh Tewari

引用次数: 0

Preparation, characterization, and application of silica nanoparticles and silica aerogel in smart drug delivery systems 二氧化硅纳米颗粒和二氧化硅气凝胶在智能给药系统中的制备、表征和应用

IF 4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Current Research in Biotechnology

Pub Date : 2025-01-01 DOI: 10.1016/j.crbiot.2025.100344

Ayda Ahmadi, Abobakr Sori, Jafarsadegh Moghaddas

Silica nanoparticles (SNPs) and silica aerogels (SAs) have emerged as new drug delivery platforms with promise for treating chronic conditions such as multidrug-resistant tuberculosis (MDR-TB) and diabetic foot infections. This review integrates papers from 2015 to 2025, selected by PubMed and Scopus searches through the use of keywords like ’silica nanoparticles drug delivery chronic infections’ to detect its significance in the fight against AMR with a maximum of 50 % enhanced drug loading efficiency. Because of their tunable porosity and favorable biocompatibility, nanoparticulate and aerogel materials are well compatible with target and controlled release of drugs, and this is how it is possible to bypass complications caused by poor drug penetration and microbial resistance. This review article, for the first time, addresses the application of silica nanoparticles and aerogels to fight drug-resistant infections in terms of scalability as well as regulatory considerations. With respect to scalability as well as regulatory, including the 2025 horizon scanning report of EMA on nanomedicines for enhanced biocompatibility and GMP scalability. It also addresses recent advances in surface modification techniques, such as covalent grafting of amine groups and non-covalent coating with polymers such as chitosan. These alterations have increased the drug loading capability (approximately 30 to 50 wt% for antibiotics) and have facilitated stimulus-responsive drug release triggered by stimuli including pH change and reactive oxygen species (ROS). The data from this review indicate that nanocarrier surface optimization has a significant ability to enhance therapeutic efficacy, wherein antibacterial action against drug-resistant microbes is increased two-fold. This review is distinct from previous structural-focused reviews because it investigates the direct relationship between drug loading capacity and surface modifications and smart drug release. Its focus on chronic drug-resistant infection renders the review an imperative reference document for guiding subsequent studies and generating clinical applications.

二氧化硅纳米颗粒（SNPs）和二氧化硅气凝胶（SAs）已经成为新的药物输送平台，有望用于治疗慢性疾病，如耐多药结核病（MDR-TB）和糖尿病足感染。本综述整合了2015年至2025年的论文，这些论文由PubMed和Scopus通过使用“二氧化硅纳米颗粒给药慢性感染”等关键词进行搜索，以检测其在对抗AMR中的意义，最多可提高50%的载药效率。由于纳米颗粒和气凝胶材料具有可调节的孔隙度和良好的生物相容性，因此它们与药物的靶标和控释具有良好的相容性，从而可以避免药物穿透性差和微生物耐药性引起的并发症。这篇综述文章首次从可扩展性和监管方面阐述了二氧化硅纳米颗粒和气凝胶在抗耐药感染方面的应用。在可扩展性和监管方面，包括2025年EMA关于纳米药物的水平扫描报告，以增强生物相容性和GMP可扩展性。它还介绍了表面改性技术的最新进展，如胺基共价接枝和壳聚糖等聚合物的非共价涂层。这些改变增加了药物装载能力（抗生素约为30%至50%），并促进了刺激反应性药物释放，包括pH变化和活性氧（ROS）。本综述的数据表明，纳米载体表面优化具有显著的提高治疗效果的能力，其中对耐药微生物的抗菌作用提高了两倍。这篇综述不同于以往以结构为重点的综述，因为它研究了药物负载能力与表面修饰和药物释放之间的直接关系。其对慢性耐药感染的关注使该综述成为指导后续研究和产生临床应用的必要参考文件。

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引用次数: 0