Plant tissue culture is an essential technique widely utilized in agriculture, horticulture, scientific research, and biodiversity preservation, offering controlled methods for plant multiplication and genetic modification. This approach contributes to increased agricultural productivity, enhanced disease resistance, and the protection of rare or threatened plant species. However, microbial contamination—originating from microorganisms in the phyllosphere, rhizosphere, and endosphere—remains a major challenge in plant micropropagation. Without effective management, contamination can inhibit regeneration, callus formation, adventitious shoot development, and may even lead to tissue death. Various strategies, including thermotherapy, chemotherapy, and cryotherapy, have been employed to combat contamination in plant micropropagation. Moreover, emerging approaches such as the application of nanotechnology and natural products offer promising, eco-friendly alternatives. This review examines the sources of contamination and highlights the strategies used to mitigate it, offering insights into innovative solutions that support sustainable agricultural practices and conservation efforts.
{"title":"Modern and traditional strategies for controlling microbial contamination in plant micropropagation: Current insights and future perspectives","authors":"Nandang Permadi , Dedat Prismantoro , Pramesthi Reitza Navisya Vasall , Farah Aprisza Sheelmarevaa , Sarifah Nurjanah , Mohamad Nurzaman , Arshad Naji Alhasnawi , Febri Doni , Euis Julaeha","doi":"10.1016/j.crbiot.2025.100337","DOIUrl":"10.1016/j.crbiot.2025.100337","url":null,"abstract":"<div><div>Plant tissue culture is an essential technique widely utilized in agriculture, horticulture, scientific research, and biodiversity preservation, offering controlled methods for plant multiplication and genetic modification. This approach contributes to increased agricultural productivity, enhanced disease resistance, and the protection of rare or threatened plant species. However, microbial contamination—originating from microorganisms in the phyllosphere, rhizosphere, and endosphere—remains a major challenge in plant micropropagation. Without effective management, contamination can inhibit regeneration, callus formation, adventitious shoot development, and may even lead to tissue death. Various strategies, including thermotherapy, chemotherapy, and cryotherapy, have been employed to combat contamination in plant micropropagation. Moreover, emerging approaches such as the application of nanotechnology and natural products offer promising, eco-friendly alternatives. This review examines the sources of contamination and highlights the strategies used to mitigate it, offering insights into innovative solutions that support sustainable agricultural practices and conservation efforts.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"10 ","pages":"Article 100337"},"PeriodicalIF":4.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145219196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Probiotics and their microbiome-derived metabolites are increasingly recognized as key players in cancer biology, functioning not only as regulators of gut homeostasis but also as modulators of tumor development, therapeutic response, and treatment-associated toxicities. These beneficial microorganisms exert their effects through diverse mechanisms, including microbiota remodeling, pathogen suppression, immune reprogramming, epigenetic regulation, and production of bioactive metabolites such as short-chain fatty acids, bacteriocins, vitamins, and exopolysaccharides. These metabolites influence fundamental processes of tumorigenesis by inducing apoptosis, inhibiting proliferation, modulating angiogenesis, and reshaping the tumor microenvironment toward a more immunoresponsive phenotype. Importantly, probiotics have been shown to enhance the efficacy of immune checkpoint inhibitors, mitigate chemotherapy- and radiotherapy-induced gastrointestinal complications, and detoxify carcinogens, thereby acting as supportive adjuncts in oncology. Emerging computational biology and multi-omics approaches—including genomics, metabolomics, and transcriptomics—have accelerated the identification of biosynthetic gene clusters and metabolic pathways responsible for anticancer bioactivity, allowing mechanistic mapping of probiotic–host–tumor interactions. Integration of these datasets highlights the potential for precision-guided probiotic formulations and engineered microbial consortia as novel therapeutic platforms. Nevertheless, clinical translation is hindered by strain-specific variability, host microbiome heterogeneity, lack of standardized protocols, and limited large-scale randomized trials. Addressing these gaps through harmonized methodologies, deeper mechanistic validation, and patient-tailored microbiome profiling will be essential for transforming probiotics from supportive dietary supplements into precision-based oncology therapeutics. This review integrates mechanistic insights, multi-omics strategies, and clinical evidence on probiotics and their metabolites in cancer therapy. By converging advances in microbial biotechnology, systems biology, and translational oncology, we propose probiotics as sustainable and precision-driven agents capable of reshaping the landscape of cancer prevention and treatment.
{"title":"Integrating probiotics and microbiome-derived metabolites into cancer therapy: mechanistic insights, multi-omics strategies, and clinical potential","authors":"Zahrasadat Seyyedi , Hamed Haddad Kashani , Atefeh Parchebafi , Romina Ghayoumi , Mohammad Mehdi Haghighat Lari , Elahe Seyed Hosseini","doi":"10.1016/j.crbiot.2025.100340","DOIUrl":"10.1016/j.crbiot.2025.100340","url":null,"abstract":"<div><div>Probiotics and their microbiome-derived metabolites are increasingly recognized as key players in cancer biology, functioning not only as regulators of gut homeostasis but also as modulators of tumor development, therapeutic response, and treatment-associated toxicities. These beneficial microorganisms exert their effects through diverse mechanisms, including microbiota remodeling, pathogen suppression, immune reprogramming, epigenetic regulation, and production of bioactive metabolites such as short-chain fatty acids, bacteriocins, vitamins, and exopolysaccharides. These metabolites influence fundamental processes of tumorigenesis by inducing apoptosis, inhibiting proliferation, modulating angiogenesis, and reshaping the tumor microenvironment toward a more immunoresponsive phenotype. Importantly, probiotics have been shown to enhance the efficacy of immune checkpoint inhibitors, mitigate chemotherapy- and radiotherapy-induced gastrointestinal complications, and detoxify carcinogens, thereby acting as supportive adjuncts in oncology. Emerging computational biology and multi-omics approaches—including genomics, metabolomics, and transcriptomics—have accelerated the identification of biosynthetic gene clusters and metabolic pathways responsible for anticancer bioactivity, allowing mechanistic mapping of probiotic–host–tumor interactions. Integration of these datasets highlights the potential for precision-guided probiotic formulations and engineered microbial consortia as novel therapeutic platforms. Nevertheless, clinical translation is hindered by strain-specific variability, host microbiome heterogeneity, lack of standardized protocols, and limited large-scale randomized trials. Addressing these gaps through harmonized methodologies, deeper mechanistic validation, and patient-tailored microbiome profiling will be essential for transforming probiotics from supportive dietary supplements into precision-based oncology therapeutics. This review integrates mechanistic insights, multi-omics strategies, and clinical evidence on probiotics and their metabolites in cancer therapy. By converging advances in microbial biotechnology, systems biology, and translational oncology, we propose probiotics as sustainable and precision-driven agents capable of reshaping the landscape of cancer prevention and treatment.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"10 ","pages":"Article 100340"},"PeriodicalIF":4.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145319558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-01DOI: 10.1016/j.crbiot.2023.100172
Pu Li , Xiaomeng Hu , Jiajia Yuan , Feiyun Sun , Pengfei Li , Wenyi Dong , Erdeng Du , Mingguo Peng
The integrated Vegetation-Activated Sludge Process (V-ASP) has emerged as a promising solution for decentralized wastewater treatment, offering a unique combination of landscape vegetation with the traditional Anaerobic-Anoxic-Oxic process (AAO). The experiments displayed that V-ASP consistently demonstrated remarkable treatment performance, maintaining stable removal efficiency exceeding 90 % for COD, NH4+-N, and TP. The vegetation growth is helpful for the removal of pollutants and the microbial community in the allocated vegetation root, while the bulk suspended sludge was changed significantly by using PCR test. To comprehensively evaluate the environmental footprint of V-ASP, a Life Cycle Assessment (LCA) was conducted. The mass balance calculation containing wastewater treatment performance, energy consumption, vegetation growth behavior, greenhouse gas (GHG) emissions, treated water, and sludge discharge was carried out to establish the life cycle inventories (LCI). SimaPro 9.0 software and the ReCiPe (H) midpoint impact assessment method were employed, which revealed that the V-ASP system boasts low GHG emissions and freshwater eutrophication potential compared to the traditional AAO process. In essence, this study provides a comprehensive understanding of the V-ASP system, especially the environmental impacts, guiding its potential for sustainable decentralized sewage treatment applications.
{"title":"Life cycle and environmental impact assessment of vegetation-activated sludge process (V-ASP) for decentralized wastewater treatment","authors":"Pu Li , Xiaomeng Hu , Jiajia Yuan , Feiyun Sun , Pengfei Li , Wenyi Dong , Erdeng Du , Mingguo Peng","doi":"10.1016/j.crbiot.2023.100172","DOIUrl":"10.1016/j.crbiot.2023.100172","url":null,"abstract":"<div><p>The integrated Vegetation-Activated Sludge Process (V-ASP) has emerged as a promising solution for decentralized wastewater treatment, offering a unique combination of landscape vegetation with the traditional Anaerobic-Anoxic-Oxic process (AAO). The experiments displayed that V-ASP consistently demonstrated remarkable treatment performance, maintaining stable removal efficiency exceeding 90 % for COD, NH<sub>4</sub><sup>+</sup>-N, and TP. The vegetation growth is helpful for the removal of pollutants and the microbial community in the allocated vegetation root, while the bulk suspended sludge was changed significantly by using PCR test. To comprehensively evaluate the environmental footprint of V-ASP, a Life Cycle Assessment (LCA) was conducted. The mass balance calculation containing wastewater treatment performance, energy consumption, vegetation growth behavior, greenhouse gas (GHG) emissions, treated water, and sludge discharge was carried out to establish the life cycle inventories (LCI). SimaPro 9.0 software and the ReCiPe (H) midpoint impact assessment method were employed, which revealed that the V-ASP system boasts low GHG emissions and freshwater eutrophication potential compared to the traditional AAO process. In essence, this study provides a comprehensive understanding of the V-ASP system, especially the environmental impacts, guiding its potential for sustainable decentralized sewage treatment applications.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100172"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262823000540/pdfft?md5=b199791f2cdc6d6f47b7448ee381f95e&pid=1-s2.0-S2590262823000540-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139023335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-01DOI: 10.1016/j.crbiot.2024.100192
Muhammad Asim Iqbal , Sabeen Gohar , Yoshinori Yabuta , Liang Fanghua , Wang Ling , Muhammad Farooq , Muhammad Nauman Sarwar , Gopiraman Mayakrishnan , Ick Soo Kim
Smart colorimetric sensor for the naked-eye detection of food freshness is considered as the most attractive tool in food safety. Herein, we prepared curcumin (CUR) coated electrospun regenerated cellulose nanofiber (RCA) composites (CUR-Cot), to monitor the real-time spoilage of raw chicken. The physicochemical properties of the CUR-Cot sensor were completely studied. The surface modification, morphology, crystalline nature, and thermal stability of CUR-Cot were investigated by using various spectral, analytical, and microscopic techniques. Based on the results, the successful coating of CUR on the surface of RCA was confirmed. Interestingly, the CUR-Cot showed a significant change in total color difference value (ΔE, 0 days − 0.0–14.93, after 1 day – 14.93–23.64, after 2 days – 23.64–44.78, after 3 days- 44.78–55.22, and after 4 days – 55.22–60.96, detectable by the naked eye) in the real-time monitoring for chicken freshness. In addition, the present CUR-Cot smart colorimetric sensor is reversible with change in pH, and the reversed sensor can be reused. Furthermore, the hydrophobic nature of the CUR-Cot was confirmed by water contact angle analysis (WCA, contact angle of 90 ± 3.00), which increases its application and adaptability. Good antibacterial, barrier, and optical properties of the present CUR-Cot were also found. Overall, the results confirm that the usage of CUR-Cot sensor can be highly efficient, user-friendly, and non-destructive for the real-time monitoring of chicken freshness.
{"title":"Green extract surface-coated electrospun cellulose nanofibers as an efficient, reversible, and reusable smart colorimetric sensor for real-time monitoring of chicken freshness","authors":"Muhammad Asim Iqbal , Sabeen Gohar , Yoshinori Yabuta , Liang Fanghua , Wang Ling , Muhammad Farooq , Muhammad Nauman Sarwar , Gopiraman Mayakrishnan , Ick Soo Kim","doi":"10.1016/j.crbiot.2024.100192","DOIUrl":"https://doi.org/10.1016/j.crbiot.2024.100192","url":null,"abstract":"<div><p>Smart colorimetric sensor for the naked-eye detection of food freshness is considered as the most attractive tool in food safety. Herein, we prepared curcumin (CUR) coated electrospun regenerated cellulose nanofiber (RCA) composites (CUR-Cot), to monitor the real-time spoilage of raw chicken. The physicochemical properties of the CUR-Cot sensor were completely studied. The surface modification, morphology, crystalline nature, and thermal stability of CUR-Cot were investigated by using various spectral, analytical, and microscopic techniques. Based on the results, the successful coating of CUR on the surface of RCA was confirmed. Interestingly, the CUR-Cot showed a significant change in total color difference value (ΔE, 0 days − 0.0–14.93, after 1 day – 14.93–23.64, after 2 days – 23.64–44.78, after 3 days- 44.78–55.22, and after 4 days – 55.22–60.96, detectable by the naked eye) in the real-time monitoring for chicken freshness. In addition, the present CUR-Cot smart colorimetric sensor is reversible with change in pH, and the reversed sensor can be reused. Furthermore, the hydrophobic nature of the CUR-Cot was confirmed by water contact angle analysis (WCA, contact angle of 90 ± 3.00), which increases its application and adaptability. Good antibacterial, barrier, and optical properties of the present CUR-Cot were also found. Overall, the results confirm that the usage of CUR-Cot sensor can be highly efficient, user-friendly, and non-destructive for the real-time monitoring of chicken freshness.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100192"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000182/pdfft?md5=273c453ff8f94e676def0bd2a303fe31&pid=1-s2.0-S2590262824000182-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140042235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-01DOI: 10.1016/j.crbiot.2024.100253
Yingjie Wu , Tao Zhou , Juanjuan Hu , Lisha Wu , Yao Hu , Liuqing Zhou
Background
Head and neck squamous cell carcinoma (HNSCC) is a common invasive malignant tumor that lacks powerful predictive or prognostic biomarkers. Ferroptosis and cuproptosis are two new forms of programmed cell death. Our study was aimed at constructing a prognostic model with a combination of cuproptosis and ferroptosis-related genes (CFRGs) for the early clinical detection of HNSCC.
Methods
We obtained the information of CFRGs, including the RNASeq data and corresponding clinical data in HNSCC patients from the TCGA and GEO databases. We assessed 28 CFRGs, and analyzed the relationship between those genes and their clinical features and prognosis of HNSCC. The consensus cluster analysis was employed to generate three CFRGclusters. Then, we investigated the association of molecular patterns and prognostic significance in these subtypes. The clinical indicators of the prognosis-related genes were identified and prognostic CFRG_score were constructed. We then built a predictive nomogram with confirmed consistency and reliability by calibration curve analysis. At last, we verified the expression of CFRGs in HNSCC tissues by qRT-PCR and immunohistochemical results.
Results
The DEGs were different between the normal and HNSCC tumor tissues and we screened out 28 CFRGs related to the prognosis in HNSCC. Associations between the clinical information and prognosis were found in the molecular subtypes related to prognosis. We utilized enrichment analysis of the differential genes and showed that those DEGs were mostly enriched in the biological processes associated with the pathways of neurodegeneration-multiple diseases, Alzheimer disease, Prion disease, Parkinson disease and Amyotrophic lateral sclerosis. CFRG_score was established to predict the survival of HNSCC patients and found that higher CFRG_score suggested favorable OS for patients, indicating the prediction of better prognosis. Moreover, we created highly reliable nomogram which could predict well for the expected prognosis. In addition, we confirmed that the expression of EGFR, VEGFA, HSPA5, SLC3A2, CAV1 and CD44 were consistent with qRT-PCR and immunohistochemical analysis in HNSCC tissues by qRT-PCR.
Conclusions
This prognostic model based on prognostic differential CFRG_score is strongly related to clinical characteristics, prognosis, and therapy in HNSCC patients and could be used as a promising tool which is dedicated to guiding the treatment of HNSCC.
{"title":"Identification of a prognostic model based on cuproptosis and ferroptosis-related genes in patients with head and neck squamous cell carcinoma","authors":"Yingjie Wu , Tao Zhou , Juanjuan Hu , Lisha Wu , Yao Hu , Liuqing Zhou","doi":"10.1016/j.crbiot.2024.100253","DOIUrl":"10.1016/j.crbiot.2024.100253","url":null,"abstract":"<div><h3>Background</h3><p>Head and neck squamous cell carcinoma (HNSCC) is a common invasive malignant tumor that lacks powerful predictive or prognostic biomarkers. Ferroptosis and cuproptosis are two new forms of programmed cell death. Our study was aimed at constructing a prognostic model with a combination of cuproptosis and ferroptosis-related genes (CFRGs) for the early clinical detection of HNSCC.</p></div><div><h3>Methods</h3><p>We obtained the information of CFRGs, including the RNASeq data and corresponding clinical data in HNSCC patients from the TCGA and GEO databases. We assessed 28 CFRGs, and analyzed the relationship between those genes and their clinical features and prognosis of HNSCC. The consensus cluster analysis was employed to generate three CFRGclusters. Then, we investigated the association of molecular patterns and prognostic significance in these subtypes. The clinical indicators of the prognosis-related genes were identified and prognostic CFRG_score were constructed. We then built a predictive nomogram with confirmed consistency and reliability by calibration curve analysis. At last, we verified the expression of CFRGs in HNSCC tissues by qRT-PCR and immunohistochemical results.</p></div><div><h3>Results</h3><p>The DEGs were different between the normal and HNSCC tumor tissues and we screened out 28 CFRGs related to the prognosis in HNSCC. Associations between the clinical information and prognosis were found in the molecular subtypes related to prognosis. We utilized enrichment analysis of the differential genes and showed that those DEGs were mostly enriched in the biological processes associated with the pathways of neurodegeneration-multiple diseases, Alzheimer disease, Prion disease, Parkinson disease and Amyotrophic lateral sclerosis. CFRG_score was established to predict the survival of HNSCC patients and found that higher CFRG_score suggested favorable OS for patients, indicating the prediction of better prognosis. Moreover, we created highly reliable nomogram which could predict well for the expected prognosis. In addition, we confirmed that the expression of EGFR, VEGFA, HSPA5, SLC3A2, CAV1 and CD44 were consistent with qRT-PCR and immunohistochemical analysis in HNSCC tissues by qRT-PCR.</p></div><div><h3>Conclusions</h3><p>This prognostic model based on prognostic differential CFRG_score is strongly related to clinical characteristics, prognosis, and therapy in HNSCC patients and could be used as a promising tool which is dedicated to guiding the treatment of HNSCC.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"8 ","pages":"Article 100253"},"PeriodicalIF":3.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000790/pdfft?md5=3b344592f4a8895e4773a2d0fd9babcf&pid=1-s2.0-S2590262824000790-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142232291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-01DOI: 10.1016/j.crbiot.2024.100259
Xiaomu Zhu , Dongdong Wang , Atanas G. Atanasov
Obesity is a condition of abnormal or excessive body fat accumulation. It is a chronic and degenerative disease that is a global health problem and causes large societal and economic burdens. However, until now, long-term pharmacotherapies to lower body weight to normal along with suitable tolerability and fewer side effects have remained a challenge. Natural products from plants are a promising source for new lead structures for drug discovery. Additionally, endogenous natural peptides have attracted increasing attention for fighting obesity recently. In this review, we first summarize recent findings on promising signaling/targets for managing body weight, including inhibition of appetite, nutrient absorption and energy expenditure. We also discuss pure natural compounds (such as betulinic acid) and endogenous peptides (such as GLP1, amylin, GIP, ghrelin) and their derivatives (such as orlistat) with anti-obesity effects.
{"title":"A review: Anti-obesity drug discovery from natural plant metabolites and endogenous peptides","authors":"Xiaomu Zhu , Dongdong Wang , Atanas G. Atanasov","doi":"10.1016/j.crbiot.2024.100259","DOIUrl":"10.1016/j.crbiot.2024.100259","url":null,"abstract":"<div><div>Obesity is a condition of abnormal or excessive body fat accumulation. It is a chronic and degenerative disease that is a global health problem and causes large societal and economic burdens. However, until now, long-term pharmacotherapies to lower body weight to normal along with suitable tolerability and fewer side effects have remained a challenge. Natural products from plants are a promising source for new lead structures for drug discovery. Additionally, endogenous natural peptides have attracted increasing attention for fighting obesity recently. In this review, we first summarize recent findings on promising signaling/targets for managing body weight, including inhibition of appetite, nutrient absorption and energy expenditure. We also discuss pure natural compounds (such as betulinic acid) and endogenous peptides (such as GLP1, amylin, GIP, ghrelin) and their derivatives (such as orlistat) with anti-obesity effects.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"8 ","pages":"Article 100259"},"PeriodicalIF":3.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142526113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In vitro studies of stone-related crystals and crystals-cell interactions have been extensively done to investigate cellular, molecular and pathogenic mechanisms leading to renal calculi. Effective preparation of various types of stone-related crystals is thus crucial for such studies. Nevertheless, various protocols for preparing these stone-related crystals were scatteredly reported without comparative analysis of their efficacies and yields. Herein, we systematically compared our protocols (with the suffix “-Si”) for preparing calcium oxalate (CaOx) monohydrate (COM), CaOx dihydrate (COD), magnesium ammonium phosphate (struvite), uric acid (UA), calcium phosphate dihydrate (brushite), hydroxyapatite (HAP), and calcium carbonate (CaCO3) crystals with other protocols published previously. The morphological evaluation revealed that our protocols provided the most homogeneous and most typical monoclinic prismatic, bipyramidal, coffin lid and rectangle shapes of COM, COD, struvite and UA crystals, respectively. There were comparable morphological results for brushite, HAP and CaCO3 crystals generated by different protocols. Our protocols provided the greatest yield for generating brushite crystals but with lower yields for others. Chemical analysis by Fourier transform infrared (FTIR) spectroscopy revealed comparable results among different protocols to generate each crystal type. In summary, all these protocols can be used to generate each crystal type. But our protocols offer the best quality, in terms of homogeneity and typical shape, for generating COM, COD, struvite and UA crystals.
为了研究导致肾结石的细胞、分子和致病机制，对结石相关晶体和晶体-细胞相互作用进行了广泛的体外研究。因此，有效制备各种类型的结石相关晶体对此类研究至关重要。然而，制备这些结石相关晶体的各种方案被零散地报道出来，却没有对其效率和产量进行比较分析。在此，我们将制备一水草酸钙（COM）、二水草酸钙（COD）、磷酸铵镁（struvite）、尿酸（UA）、二水磷酸钙（brushite）、羟基磷灰石（HAP）和碳酸钙（CaCO3）晶体的方案（后缀为"-Si"）与之前发表的其他方案进行了系统比较。形态评估结果显示，我们的方案分别获得了最均匀、最典型的单斜棱柱形、双棱柱形、棺盖形和矩形的 COM、COD、硬石膏和 UA 晶体。不同方案生成的刷石、HAP 和 CaCO3 晶体的形态结果相当。我们的方案生成的刷石晶体产量最高，但其他晶体的产量较低。通过傅立叶变换红外光谱（FTIR）进行的化学分析显示，不同方案生成每种晶体类型的结果相当。总之，所有这些方案都可用于生成每种晶体类型。但就均匀性和典型形状而言，我们的方案在生成 COM、COD、闪石和 UA 晶体方面质量最好。
{"title":"Systematic comparisons of preparative protocols to generate various types of stone-related crystals for in vitro study of renal calculi","authors":"Niracha Koeipudsa, Paleerath Peerapen, Visith Thongboonkerd","doi":"10.1016/j.crbiot.2024.100239","DOIUrl":"10.1016/j.crbiot.2024.100239","url":null,"abstract":"<div><p><em>In vitro</em> studies of stone-related crystals and crystals-cell interactions have been extensively done to investigate cellular, molecular and pathogenic mechanisms leading to renal calculi. Effective preparation of various types of stone-related crystals is thus crucial for such studies. Nevertheless, various protocols for preparing these stone-related crystals were scatteredly reported without comparative analysis of their efficacies and yields. Herein, we systematically compared our protocols (with the suffix “-Si”) for preparing calcium oxalate (CaOx) monohydrate (COM), CaOx dihydrate (COD), magnesium ammonium phosphate (struvite), uric acid (UA), calcium phosphate dihydrate (brushite), hydroxyapatite (HAP), and calcium carbonate (CaCO<sub>3</sub>) crystals with other protocols published previously. The morphological evaluation revealed that our protocols provided the most homogeneous and most typical monoclinic prismatic, bipyramidal, coffin lid and rectangle shapes of COM, COD, struvite and UA crystals, respectively. There were comparable morphological results for brushite, HAP and CaCO<sub>3</sub> crystals generated by different protocols. Our protocols provided the greatest yield for generating brushite crystals but with lower yields for others. Chemical analysis by Fourier transform infrared (FTIR) spectroscopy revealed comparable results among different protocols to generate each crystal type. In summary, all these protocols can be used to generate each crystal type. But our protocols offer the best quality, in terms of homogeneity and typical shape, for generating COM, COD, struvite and UA crystals.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"8 ","pages":"Article 100239"},"PeriodicalIF":3.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000650/pdfft?md5=dd85f9bd8d02cea1de220d5e9b696334&pid=1-s2.0-S2590262824000650-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141852088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-01DOI: 10.1016/j.crbiot.2024.100220
Preethy P. Raj , Rajesh Kanna Gopal , Elumalai Sanniyasi
Severe inflammation in joints caused by the detrimental effects of the immune system is termed Rheumatoid arthritis. The unconstrained proliferation of immune cells and pro-inflammatory cytokines deteriorates Synovium which secretes synovial fluid to lubricate joints and cartilage. Non-steroidal anti-inflammatory drugs (NSAIDs) are the only therapeutics for treating rheumatoid arthritis, and long-term intake causes serious side effects on the organs. Fucoidan, a sulfated polysaccharide found on the cell walls of brown algae shows bioactive potential. In our study, fucoidan was extracted from Padina pavonica (PD), Stoechospermum marginatum (StM), Spatolossum macrodontum (SpM), Dictyota bartayresiana (DD), and Turbinaria decurrens (TD) and evaluated for anti-inflammatory and anti-arthritis activities. Fucoidan was extracted and evaluated for anti-inflammatory activity in vitro using RAW 264.7 macrophage cell lines, followed by in vivo anti-arthritis activity on Wistar male rats. Nitric oxide suppression was comparatively high in fucoidan from TD (IC50 − 12.93 µg/mL). Purified fucoidan from TD, significantly reduced inflammation, size of paw edema, downregulated proinflammatory cytokines (IL-6, IL-1β, TNF-α), and upregulated anti-inflammatory cytokine (IL10) in CFA-induced arthritis in Wistar male rats. Biochemical parameters like SOD, CAT, GSH, GPX, and GST and haematological parameters like total-protein, albumin, haemoglobin, and RBC were upregulated, and other parameters like urea, uric acid, creatinine, bilirubin, SGOT, SGPT, ALP, WBC, ESR, RF, and CRP were downregulated. Histopathology of the liver, kidney, and ankle joints reveals that fucoidan intake restrained inflammation and tissue damage. Therefore, fucoidan extracted from TD is a potential candidate for the treatment of rheumatoid arthritis.
{"title":"Investigating the anti-inflammatory and anti-arthritis effects of fucoidan from a brown seaweed","authors":"Preethy P. Raj , Rajesh Kanna Gopal , Elumalai Sanniyasi","doi":"10.1016/j.crbiot.2024.100220","DOIUrl":"https://doi.org/10.1016/j.crbiot.2024.100220","url":null,"abstract":"<div><p>Severe inflammation in joints caused by the detrimental effects of the immune system is termed Rheumatoid arthritis. The unconstrained proliferation of immune cells and pro-inflammatory cytokines deteriorates Synovium which secretes synovial fluid to lubricate joints and cartilage. Non-steroidal anti-inflammatory drugs (NSAIDs) are the only therapeutics for treating rheumatoid arthritis, and long-term intake causes serious side effects on the organs. Fucoidan, a sulfated polysaccharide found on the cell walls of brown algae shows bioactive potential. In our study, fucoidan was extracted from <em>Padina pavonica</em> (PD), <em>Stoechospermum marginatum</em> (StM), <em>Spatolossum macrodontum</em> (SpM), <em>Dictyota bartayresiana</em> (DD), and <em>Turbinaria decurrens</em> (TD) and evaluated for anti-inflammatory and anti-arthritis activities. Fucoidan was extracted and evaluated for anti-inflammatory activity <em>in vitro</em> using RAW 264.7 macrophage cell lines, followed by <em>in vivo</em> anti-arthritis activity on Wistar male rats. Nitric oxide suppression was comparatively high in fucoidan from TD (IC<sub>50</sub> − 12.93 µg/mL). Purified fucoidan from TD, significantly reduced inflammation, size of paw edema, downregulated proinflammatory cytokines (IL-6, IL-1β, TNF-α), and upregulated anti-inflammatory cytokine (IL10) in CFA-induced arthritis in Wistar male rats. Biochemical parameters like SOD, CAT, GSH, GPX, and GST and haematological parameters like total-protein, albumin, haemoglobin, and RBC were upregulated, and other parameters like urea, uric acid, creatinine, bilirubin, SGOT, SGPT, ALP, WBC, ESR, RF, and CRP were downregulated. Histopathology of the liver, kidney, and ankle joints reveals that fucoidan intake restrained inflammation and tissue damage. Therefore, fucoidan extracted from TD is a potential candidate for the treatment of rheumatoid arthritis.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100220"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000467/pdfft?md5=e3746c4e2d04e126ed16f7ed27d36772&pid=1-s2.0-S2590262824000467-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140901381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-01DOI: 10.1016/j.crbiot.2024.100229
Albert M Liao , Shailaja Agrawal , James Cory Benson , G. Thomas Caltagirone
Creatine kinase-MM (CK-MM) is a relatively muscle-specific enzyme with a plasma half-life of approximately 2 h. Total creatine kinase elevation is evident in several conditions associated with acute muscle injury, severe muscular exertion, and myocardial infarction. The presence of a large amount of the CK-MM enzyme in blood serum is a biomarker of muscular injuries and cardiac assault. In this study, we developed a structure-switching aptamer that can be immobilized on a sensor to detect CK-MM. CK-BB was used as a counter-target to ensure the specific targeting of CK-MM. Melting-Off SELEX was employed to develop aptamers exhibiting significant structural changes on binding. The selected aptamer shows a high affinity towards CK-MM with a Kd value of 14.7 nM.
{"title":"Selection of aptamer for creatine Kinase-MM as a marker for muscular dystrophy and myocardial infarction","authors":"Albert M Liao , Shailaja Agrawal , James Cory Benson , G. Thomas Caltagirone","doi":"10.1016/j.crbiot.2024.100229","DOIUrl":"https://doi.org/10.1016/j.crbiot.2024.100229","url":null,"abstract":"<div><p>Creatine kinase-MM (CK-MM) is a relatively muscle-specific enzyme with a plasma half-life of approximately 2 h. Total creatine kinase elevation is evident in several conditions associated with acute muscle injury, severe muscular exertion, and myocardial infarction. The presence of a large amount of the CK-MM enzyme in blood serum is a biomarker of muscular injuries and cardiac assault. In this study, we developed a structure-switching aptamer that can be immobilized on a sensor to detect CK-MM. CK-BB was used as a counter-target to ensure the specific targeting of CK-MM. Melting-Off SELEX was employed to develop aptamers exhibiting significant structural changes on binding. The selected aptamer shows a high affinity towards CK-MM with a K<sub>d</sub> value of 14.7 nM.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100229"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000558/pdfft?md5=f3b0eaa258043b204aeb1269ea2335fb&pid=1-s2.0-S2590262824000558-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141240229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-01DOI: 10.1016/j.crbiot.2024.100184
Saikat Sena , Soni Kumari , Vijay Kumar , Azamal Husen
Light quality (spectral arrangement) and quantity (photoperiod and intensity) influence plant growth and metabolism and also interact with several factors including environmental parameters in defining the plant behavior. The Light Emitting Diode (LED) lights are extensively utilized in the cultivation of several plant species, especially horticultural plants due to their lower power consumption and higher luminous efficiency compared to the conventional fluorescent lights. The aim of this review paper is to examine the potential of LED technology as it relates to plant lighting in greenhouses and other horticultural environments. It also desires to give an in-depth study of the advantages of LED lighting on plant development, yield, the production of secondary metabolites, and defense mechanisms. Horticultural lighting might undergo a revolution because LEDs are used in solid-state lighting, which would be a tremendous advancement after decades of research. LEDs may be used in a variety of horticulture lighting applications, such as tissue culture lighting, controlled environment research lighting, supplementary lighting, and photoperiod lighting for greenhouses. The primary impacts of light colors on plant performance are shown by the spectrum effects of LEDs as an independent source of light, together with the diverse sensitivity of many plant species and alternatives. LED light influences performance of enzyme, gene expression, cell wall formation, plant defense and postharvest quality. The spectrum reactions are mediated by the ambient lighting in a greenhouse, which also indicates a strong relationship between the additional supplementary lighting and changing environmental factors. LEDs are growing further to become cost-effective for even large-scale horticulture lighting applications as light output increases and device expenditures decrease.
光的质量（光谱排列）和数量（光周期和强度）影响植物的生长和新陈代谢，并与包括环境参数在内的多种因素相互作用，从而决定植物的行为。与传统荧光灯相比，发光二极管（LED）灯耗电量更低，发光效率更高，因此被广泛用于多种植物的栽培，尤其是园艺植物。本文旨在研究 LED 技术在温室和其他园艺环境中植物照明方面的潜力。本文还希望深入研究 LED 照明在植物生长发育、产量、次生代谢产物的产生和防御机制方面的优势。园艺照明可能会发生一场革命，因为 LED 被用于固态照明，这将是几十年研究之后的巨大进步。LED 可用于各种园艺照明应用，如组织培养照明、受控环境研究照明、辅助照明和温室的光周期照明。光色对植物性能的主要影响体现在 LED 作为独立光源的光谱效应，以及许多植物物种和替代品的不同敏感性。LED 光对酶的性能、基因表达、细胞壁的形成、植物防御和采后质量都有影响。光谱反应受温室环境光照的影响，这也表明额外的补充光照与不断变化的环境因素之间存在密切关系。随着光输出的增加和设备成本的降低，LED 在大规模园艺照明应用中的成本效益正在进一步提高。
{"title":"Light emitting diode (LED) lights for the improvement of plant performance and production: A comprehensive review","authors":"Saikat Sena , Soni Kumari , Vijay Kumar , Azamal Husen","doi":"10.1016/j.crbiot.2024.100184","DOIUrl":"https://doi.org/10.1016/j.crbiot.2024.100184","url":null,"abstract":"<div><p>Light quality (spectral arrangement) and quantity (photoperiod and intensity) influence plant growth and metabolism and also interact with several factors including environmental parameters in defining the plant behavior. The Light Emitting Diode (LED) lights are extensively utilized in the cultivation of several plant species, especially horticultural plants due to their lower power consumption and higher luminous efficiency compared to the conventional fluorescent lights. The aim of this review paper is to examine the potential of LED technology as it relates to plant lighting in greenhouses and other horticultural environments. It also desires to give an in-depth study of the advantages of LED lighting on plant development, yield, the production of secondary metabolites, and defense mechanisms. Horticultural lighting might undergo a revolution because LEDs are used in solid-state lighting, which would be a tremendous advancement after decades of research. LEDs may be used in a variety of horticulture lighting applications, such as tissue culture lighting, controlled environment research lighting, supplementary lighting, and photoperiod lighting for greenhouses. The primary impacts of light colors on plant performance are shown by the spectrum effects of LEDs as an independent source of light, together with the diverse sensitivity of many plant species and alternatives. LED light influences performance of enzyme, gene expression, cell wall formation, plant defense and postharvest quality. The spectrum reactions are mediated by the ambient lighting in a greenhouse, which also indicates a strong relationship between the additional supplementary lighting and changing environmental factors. LEDs are growing further to become cost-effective for even large-scale horticulture lighting applications as light output increases and device expenditures decrease.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100184"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000108/pdfft?md5=45bc5c6f3ec8a6e97e7afac6a34cf132&pid=1-s2.0-S2590262824000108-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139737873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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