Current Research in Biotechnology最新文献

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IF 4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Current Research in Biotechnology

Pub Date : 2025-01-01

引用次数: 0

IF 4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Current Research in Biotechnology

Pub Date : 2025-01-01

引用次数: 0

IF 4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Current Research in Biotechnology

Pub Date : 2025-01-01

引用次数: 0

IF 4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Current Research in Biotechnology

Pub Date : 2025-01-01

引用次数: 0

Ranking economic and environmental performance of feedstocks used in bio-based production systems 对生物基生产系统中使用的原料的经济和环境性能进行排名

IF 3.6 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Current Research in Biotechnology

Pub Date : 2025-01-01 DOI: 10.1016/j.crbiot.2025.100275

Dania Muhieddine Orfali , Samir Meramo , Sumesh Sukumara

Biotechnology offers renewable alternatives for producing food, materials, and numerous functional compounds. While rampant human activities are disrupting planets’ geophysical flows, it is urgent to develop sustainable solutions with novel feedstocks and innovative valorization pathways. With the need to reduce greenhouse gas emissions and enhance circularity, new raw materials termed the next-generation feedstocks (NGFs), such as carbon dioxide, methane, methanol, formic acid, and acetic acid, have emerged as potential feedstocks for bio-based processes. So far, no such review exists that compares the performance of conventional, sugar, lignocellulosic, algae-based feedstocks, and NGFs, which biotechnology could upcycle into a wide range of products. In this review, the economic and environmental performances of the feedstocks are analyzed, and quantifications are presented and standardized based on techno-economic analysis and life cycle assessment models. The main parameters for comparison included the geographical location, unit production cost, and environmental impact categories. The results show that the economic and environmental performances are highly variable among the different feedstocks and their processing routes, also depicting evident tradeoffs. Carbon dioxide, sugar cane molasses and glycerol from waste streams are performing better on assessed indicators overall than other potential feedstocks. Nonetheless, this designed data source is the first step for reliable feedstock selection based on sustainability criteria.

生物技术为生产食品、材料和许多功能性化合物提供了可再生的替代品。虽然猖獗的人类活动正在破坏地球的地球物理流动，但迫切需要开发具有新型原料和创新增值途径的可持续解决方案。随着减少温室气体排放和提高循环性的需要，被称为下一代原料（NGFs）的新原料，如二氧化碳、甲烷、甲醇、甲酸和乙酸，已经成为生物基工艺的潜在原料。到目前为止，还没有这样的综述对传统的、糖的、木质纤维素的、藻类的原料和NGFs的性能进行比较，生物技术可以将其升级为多种产品。本文对原料的经济和环境性能进行了分析，并基于技术经济分析和生命周期评价模型进行了量化和标准化。比较的主要参数包括地理位置、单位生产成本和环境影响类别。结果表明，不同原料及其加工路线的经济和环境性能变化很大，也表现出明显的权衡。二氧化碳、甘蔗糖蜜和来自废物流的甘油在评估指标上的总体表现优于其他潜在原料。尽管如此，这个设计的数据源是基于可持续性标准进行可靠原料选择的第一步。

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引用次数: 0

Corrigendum to “Synthesis and analgesic activity of new analogs of FELL tetrapeptide containing D-Phe in the first position” [Curr. Res. Biotechnol. 8 (2024) 100249] “含d -苯丙氨酸的新型FELL四肽类似物的合成和镇痛活性”的更正[Curr.]。生物技术学报，8 (2024)100249 [j]

IF 3.6 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Current Research in Biotechnology

Pub Date : 2025-01-01 DOI: 10.1016/j.crbiot.2025.100283

Boryana Borisova , Hristina Nocheva , Ivan Iliev , Marie Laronze-Cochard , Stéphane Gérard , Stoyko Petrin , Dancho Danalev

引用次数: 0

Validation of adventitious agent safety in Yimmugo®, a novel IVIG preparation for human use 新型人用IVIG制剂Yimmugo®的安全性验证

IF 4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Current Research in Biotechnology

Pub Date : 2025-01-01 DOI: 10.1016/j.crbiot.2025.100333

Gerhard Poelsler, Marcel Asper, Sebastian Lülf, Florian Zirkel

Intravenous infusion of human IgG (IVIG) is of vital importance for patients suffering from primary or secondary immunodeficiency syndromes, but also has been found to be clinically beneficial in other diseases with autoimmunogenic or inflammatory background. Its clinical application and therefore its demand have been continuously rising during the last decades. Besides IgG, other human antibody classes have also entered clinical applications, therefore a novel manufacturing procedure has been conceived which allows simultaneous isolation of two immunoglobulin preparations, Yimmugo™ (an IVIG) and trimodulin (an IgM concentrate). Since the only suitable source for these antibodies is human donor plasma, from which it is isolated using a series of dedicated purification steps, there is a risk of carrying over infectious human pathogens into the final preparations. We describe here validation of the measures taken to provide Yimmugo, an IVIG product, with robust margins of biological safety, free of pathogens of microbial, viral or prion origin. To this end, we spiked manufacturing process intermediates of Yimmugo with diverse pathogens and tested the capacity of the manufacturing steps to clear these from the preparation. We show that four different purification steps of the Yimmugo procedure efficiently confer clearance of viral and prion pathogens, thereby providing a safe product even in the hypothetical case that an infectious agent in the original material were present. Consequently, the novel preparation procedure yields a provably safe product and simultaneously allows production of an additional medicine from the same plasma pool.

静脉输注人IgG （IVIG）对原发性或继发性免疫缺陷综合征患者至关重要，但在临床上也发现对其他具有自身免疫原性或炎症背景的疾病有益。近几十年来，其临床应用和需求不断上升。除了IgG，其他人类抗体类别也已进入临床应用，因此，一种新的制造工艺已被设想，可以同时分离两种免疫球蛋白制剂，Yimmugo™（IVIG）和trimodulin （IgM浓缩物）。由于这些抗体的唯一合适来源是人类供体血浆，抗体是通过一系列专门的纯化步骤从血浆中分离出来的，因此存在将传染性人类病原体带入最终制备的风险。我们在这里描述了为提供Yimmugo所采取的措施的验证，Yimmugo是一种IVIG产品，具有强大的生物安全性，不含微生物、病毒或朊病毒来源的病原体。为此，我们在Yimmugo的生产过程中间体中加入了多种病原体，并测试了生产步骤从制剂中清除这些病原体的能力。我们表明，四种不同的纯化步骤的Yimmugo程序有效地授予清除病毒和朊病毒病原体，从而提供了一个安全的产品，即使在假设的情况下，在原始材料中存在传染因子。因此，新的制备程序产生可证明安全的产品，并同时允许从相同的血浆池生产额外的药物。

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引用次数: 0

Multifaceted microbial enzyme nattokinase: a comprehensive review on therapeutics applications, production technologies and intellectual property landscape 多面微生物酶纳豆激酶：治疗应用、生产技术和知识产权格局的综合综述

IF 3.6 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Current Research in Biotechnology

Pub Date : 2025-01-01 DOI: 10.1016/j.crbiot.2025.100316

Tirth Chetankumar Bhatt , Viralkumar B. Mandaliya , Munir Ibrahim , Avani Bhimani , Asmita Detroja , Jaykumar Koradiya , Gaurav Sanghvi , Ashok Kumar Bishoyi

Cardiovascular diseases (CVDs) have risen drastically after the COVID era as post-COVID symptoms. “Nattokinase” (NK), the thrombolytic enzyme, is known for its substrate-specific thrombolytic, protease, and fibrinolytic activity to ameliorate CVDs. Primarily, NK was isolated from Bacillus subtilis natto using either solid- or liquid-state fermentation technology. Later, the researchers studied the arpN gene, which encodes NK and is used in various biotechnological approaches with different hosts such as Bacillus spp., Escherichia coli, Lactobacillus spp., and Pichia pastoris. The global market potential of NK in treating CVDs has recently garnered attention, leading to a demand for bulk production and ultrapure forms of NK. This demand has put pressure on strain improvements and innovation in production technology. The patent scenario suggested that NK have been given more attention with 763 patents compared to streptokinase, staphylokinase and serratiopeptidase. This review critically examined the biotechnological aspects of NK in amelioration of CVDs, focusing on current production technologies, strain improvement strategies, protected NK innovations, and future research directions. It emphasizes the need for advanced approaches like CRISPR technology and novel production protocols to develop high-quality, ultrapure NK. It is also aligning with Sustainable Development Goal (SDG) 3: “Good Health and Well-Being.”

新型冠状病毒感染症（COVID - 19）后，心血管疾病（cvd）急剧增加。“纳豆激酶”（NK）是一种溶栓酶，以其底物特异性溶栓、蛋白酶和纤溶活性而闻名，可改善心血管疾病。首先，采用固态和液态发酵技术从纳豆枯草芽孢杆菌中分离NK。随后，研究人员研究了编码NK的arpN基因，该基因被用于不同宿主的各种生物技术方法，如芽孢杆菌、大肠杆菌、乳杆菌和毕赤酵母。NK在治疗心血管疾病方面的全球市场潜力最近引起了人们的关注，导致对批量生产和超纯NK的需求。这种需求给生产技术的改进和创新带来了压力。从专利情况来看，NK比链激酶、葡萄激酶和锯齿肽酶更受关注，共有763项专利。本文综述了生物技术方面NK在cvd改良中的应用，重点介绍了目前的生产技术、菌株改良策略、保护NK创新和未来的研究方向。它强调需要先进的方法，如CRISPR技术和新的生产方案，以开发高质量，超纯NK。它也符合可持续发展目标3：“良好健康和福祉”。

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引用次数: 0

Green-synthesized nano-fertilizers: a sustainable approach to enhancing crop productivity and stress tolerance 绿色合成纳米肥料：提高作物生产力和抗逆性的可持续途径

IF 4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Current Research in Biotechnology

Pub Date : 2025-01-01 DOI: 10.1016/j.crbiot.2025.100345

Shefali Thanta , Garima Bhanwala , Neetika Kimta , Vishnu D. Rajput , Tabarak Malik

Growing global food demand and stagnant yields caused by nutrient inefficiencies threaten sustainable agriculture. Conventional chemical fertilizers, while increasing crop productivity, often alters soil physicochemical and biological properties and cause environmental damage, highlighting a critical gap in sustainable crop nutrition. Moreover, conventional application methods are causing chemical overdosing, leading to eutrophication, nitrogen volatilization, and progressive release of greenhouse gases. In contrast, nanotechnology provides a groundbreaking solution. Nano-fertilizers (NFs) serve as concentrated sources of plant nutrients, encapsulated in nanomaterials that easily pass through stomata and root hairs, and penetrate deeper into plant tissues. This significantly decreases nutrient loss, enhances nutrient efficiency, thereby boosting plant productivity. The green synthesis of NFs uses eco-friendly plant extracts as reducing agents, and their high solubility allows for easy foliar and root application. This review offers a structured evaluation of the potential for green-synthesized NFs as effective, environmentally friendly alternatives. Green NFs significantly boost crop yields compared to conventional chemical fertilizers, while also helping to mitigate plant stress caused by biotic and abiotic factors. Furthermore, with additional long-term research, commercial scaling, and regulatory support, green NFs could transform fertilization practices. Moreover, this also provides strategic insights to address nutrient use inefficiencies and promote a more sustainable ecosystem.

全球粮食需求的增长和营养效率低下导致的产量停滞威胁着可持续农业。常规化肥在提高作物生产力的同时，往往会改变土壤的物理化学和生物特性，并造成环境破坏，突出了可持续作物营养方面的重大空白。此外，传统的施用方法导致化学物质过量，导致富营养化、氮挥发和温室气体的逐步释放。相比之下，纳米技术提供了一个突破性的解决方案。纳米肥料（NFs）作为植物营养物质的浓缩来源，被包裹在纳米材料中，很容易通过气孔和根毛，并深入植物组织。这大大减少了养分损失，提高了养分效率，从而提高了植物生产力。NFs的绿色合成使用环保植物提取物作为还原剂，其高溶解度允许易于叶面和根部应用。这篇综述对绿色合成的NFs作为有效的、环境友好的替代品的潜力进行了结构化的评估。与传统化学肥料相比，绿色氮肥显著提高作物产量，同时也有助于减轻生物和非生物因素造成的植物胁迫。此外，通过额外的长期研究、商业规模和监管支持，绿色NFs可以改变施肥实践。此外，这也为解决养分利用效率低下和促进更可持续的生态系统提供了战略见解。

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IF 4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Current Research in Biotechnology

Pub Date : 2025-01-01

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