Current Research in Biotechnology最新文献_第4页

Integrating biotechnology into diagnostic labs: Innovations in PCR and molecular procedures 将生物技术整合到诊断实验室：PCR和分子程序的创新

IF 4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Current Research in Biotechnology

Pub Date : 2025-01-01 DOI: 10.1016/j.crbiot.2025.100325

Muhammad Faran Tahir , Sidra Ishtiaq , Muhammad Anas , Ayesha Irfan , Mariusz Jaremko , Shah Fahad , Sezai Ercişli , Hanan Almahasheer , Nader R. Abdelsalam

The field of biotechnology has significantly contributed to progress in diagnostic laboratories, especially in the early identification of genetic disorders. Timely detection plays a vital role in the treatment and management of such conditions, and numerous molecular approaches have been established to identify disease markers with high accuracy and sensitivity. The polymerase chain reaction (PCR), a cornerstone technique in molecular biology, has seen various advancements, establishing it as an essential method in diagnostic settings for recognizing genetic abnormalities. PCR-based approaches have transformed the diagnostic process by amplifying trace amounts of DNA, allowing for the identification of mutations linked to a wide range of inherited conditions. This review explores the role of biotechnology in diagnostic labs, emphasizing genetic disease detection through molecular procedures. Special attention is given to innovations in PCR, highlighting new PCR-based techniques such as digital PCR, multiplex PCR, and reverse-transcription PCR, which have improved sensitivity and versatility. Additionally, various PCR methodologies, their diagnostic applications, and inherent limitations are discussed. The role of molecular tools in identifying diseases such as cancer, hereditary disorders, and infectious infections is also explored. The review further outlines the evolution of PCR technologies, their integration into molecular diagnostics, and their potential to improve diagnostic accuracy and enable early disease detection. Ongoing advancements in PCR technologies are transforming diagnostic practices by enabling more accurate, faster, and widely accessible molecular testing. These innovations promise enhanced disease management and better clinical outcomes, heralding a new era in healthcare.

生物技术领域极大地促进了诊断实验室的进展，特别是在遗传疾病的早期鉴定方面。及时发现在治疗和管理这些疾病中起着至关重要的作用，并且已经建立了许多分子方法来准确和敏感地识别疾病标志物。聚合酶链反应（PCR）是分子生物学的基础技术，已经取得了各种进展，使其成为识别遗传异常诊断环境中的基本方法。基于聚合酶链反应（pcr）的方法通过扩增微量DNA，从而改变了诊断过程，从而可以识别与多种遗传疾病相关的突变。这篇综述探讨了生物技术在诊断实验室中的作用，强调通过分子程序检测遗传病。特别关注PCR的创新，强调新的基于PCR的技术，如数字PCR，多重PCR和反转录PCR，这些技术提高了灵敏度和多功能性。此外，各种PCR方法，他们的诊断应用和固有的局限性进行了讨论。分子工具在识别疾病，如癌症，遗传性疾病和感染性感染的作用也进行了探讨。这篇综述进一步概述了PCR技术的发展，它们与分子诊断的结合，以及它们在提高诊断准确性和实现疾病早期检测方面的潜力。PCR技术的不断进步正在改变诊断实践，使分子检测更准确、更快速、更容易获得。这些创新承诺加强疾病管理和更好的临床结果，预示着医疗保健的新时代。

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引用次数: 0

A study on the impact of electrode and membrane modification in stacked microbial fuel cells for wastewater treatment 电极和膜改性对堆垛式微生物燃料电池废水处理的影响研究

IF 3.6 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Current Research in Biotechnology

Pub Date : 2025-01-01 DOI: 10.1016/j.crbiot.2025.100278

Aritro Banerjee, Rajnish Kaur Calay, Somil Thakur, Mohamad Y. Mustafa

This study investigates the efficacy of treating wastewater using microbial fuel cell (MFC) technology to the safe limits for discharge in the environment. It has been demonstrated that MFC directly converts organic matter present in wastewater into energy. The present study uses a cell design based on simple plate geometry, carbon felt electrodes and Nafion117 as proton exchange membrane separating the anode and cathode chambers. The anode was then modified with heat and acid treatment and PEM was treated with PVDF to improve the performance of the cell. Synthetic dairy wastewater with initial COD of 2412 mg/l was used to test the operation of stack consisting of four cells which were hydraulically connected in series. The stack operated with continuous flow of wastewater. COD removal of the feed water was tested in successive cells to achieve the permissible limits for safe discharge of the effluent. COD decreased from 2412 mg/l to 126 mg/l after the fourth cell. For the power output each cell was treated individually. The power density of each cell was directly proportional to the COD of the influent. The power density of the first cell that has the highest COD was measured at 77.9 mW/m², which is two times that for the cell with unmodified anode and membrane. For the first cell COD removal was the highest at 57 % and 2.6 times more than the cell with the unmodified anode and membrane. These results suggest that targeted modifications to the anode and membrane can significantly boost the MFC performance both in terms of COD removal and corresponding power output. Secondly, up to 93.66 % COD removal may be achieved by four cells hydraulically connected in series. The paper offers some insights for stacking options for implementing at scale up of the MFC technology for wastewater treatment plants.

本研究探讨了微生物燃料电池（MFC）技术处理废水的效果，使其达到环境排放的安全限值。研究表明，MFC可直接将废水中的有机物转化为能源。本研究采用基于简单板几何的电池设计，碳毡电极和Nafion117作为质子交换膜分离阳极和阴极室。然后对阳极进行热处理和酸处理，对PEM进行PVDF处理，以提高电池的性能。以初始COD为2412 mg/l的乳业合成废水为试验材料，采用水力串联的4个池组成的反应器进行运行试验。这个烟囱是在污水不断流入的情况下运行的。在连续的单元中测试了对给水的COD去除，以达到安全排放出水的允许限度。COD由2412mg /l降至126mg /l。对于功率输出，每个单元被单独处理。各池的功率密度与进水COD成正比。COD最高的第一个电池的功率密度为77.9 mW/m2，是未修饰阳极和膜的电池的2倍。第一个电池的COD去除率最高，达到57%，是未修饰阳极和膜的2.6倍。这些结果表明，对阳极和膜进行针对性的修饰可以显著提高MFC的COD去除率和相应的功率输出。其次，采用液压串联4个电解槽，COD去除率可达93.66%。本文为污水处理厂大规模实施MFC技术的堆垛方案提供了一些见解。

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引用次数: 0

Bromelain as a multifunctional anticancer agent: targeting proliferation, migration, and apoptosis in breast and liver cancer cells 菠萝蛋白酶作为一种多功能抗癌剂：靶向乳腺癌和肝癌细胞的增殖、迁移和凋亡

IF 4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Current Research in Biotechnology

Pub Date : 2025-01-01 DOI: 10.1016/j.crbiot.2025.100341

Nehad A. Shaer, Nouf S. Al-Abbas

This study investigates the anticancer potential of bromelain, a natural proteolytic enzyme derived from pineapple stems, against two of the most prevalent cancers in Saudi Arabia: MCF-7 breast cancer and HepG-2 liver cancer cells. Cell viability was assessed by MTT assay following treatment with increasing concentrations of bromelain for 24 and 48 h, with Taxol serving as a positive control. The anti-migratory effect was evaluated by wound-healing assays, while apoptosis was quantified using Annexin V/7-AAD staining and flow cytometry. Expression of apoptosis-related genes (BAX and BCL2) was analyzed by real-time PCR. Bromelain significantly reduced cancer cell proliferation and migration in a dose- and time-dependent manner, induced both apoptotic and necrotic cell death, and shifted the BAX/BCL2 ratio toward apoptosis. Collectively, these findings demonstrate that bromelain exerts multi-targeted anticancer effects and highlight its potential as a safe, natural adjunct or alternative to conventional chemotherapy in breast and liver cancers.

这项研究调查了菠萝蛋白酶的抗癌潜力，菠萝蛋白酶是一种从菠萝茎中提取的天然蛋白水解酶，可以对抗沙特阿拉伯最常见的两种癌症：MCF-7乳腺癌和HepG-2肝癌细胞。以紫杉醇为阳性对照，增加菠萝蛋白酶浓度处理24和48小时后，采用MTT法评估细胞活力。采用膜联蛋白V/7-AAD染色和流式细胞术检测细胞凋亡。实时荧光定量PCR检测细胞凋亡相关基因BAX和BCL2的表达。菠萝蛋白酶以剂量和时间依赖的方式显著降低癌细胞的增殖和迁移，诱导凋亡和坏死细胞死亡，并使BAX/BCL2比值偏向凋亡。总的来说，这些发现表明菠萝蛋白酶具有多靶点的抗癌作用，并突出了其作为乳腺癌和肝癌常规化疗的安全，天然辅助或替代方案的潜力。

引用次数: 0

Advancing recombinant protein production by bacteria: strategies and challenges in heterologous systems 推进重组蛋白的细菌生产：策略和挑战在异源系统

IF 4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Current Research in Biotechnology

Pub Date : 2025-01-01 DOI: 10.1016/j.crbiot.2025.100342

Devtulya Chander , Diksha Koul , Arushe Tickoo , Asha Chaubey

Recombinant protein production in bacteria is pivotal in industrial biotechnology, which enables scalable, cost-effective, and efficient synthesis of therapeutic proteins and enzymes. Present review explores key bacterial hosts including Escherichia coli, Bacillus subtilis, Bacillus licheniformis, and Brevibacillus choshinensis and more with an emphasis on extracellular heterologous protein production. Herein,we focus on secretion pathways such as Sec, Twin-arginine translocation (Tat) pathway, and ATP Binding Cassette (ABC) transporter systems, which govern protein export across membranes. The molecular strategies ranging from promoter optimization and codon adaptation to signal peptide engineering, strain modification, as well as protein fusion techniques have been highlighted that lead to improved secretion efficiency and product yields. Comparative insights into Gram-positive and Gram-negative systems, along with case studies, as well as importance of tailored approaches for different hosts and proteins have been reviewed. The review further discusses the growing relevance of CRISPR/Cas9n-based genetic interventions and the role of microbial physiology in facilitating optimized protein folding and transport. Overall, the integration of synthetic biology, secretion engineering, and host optimization offers promising avenues for advancing the extracellular production of high-value recombinant proteins.

在细菌中生产重组蛋白是工业生物技术的关键，它使治疗蛋白和酶的合成具有可扩展性、成本效益和效率。本文综述了大肠杆菌、枯草芽孢杆菌、地衣芽孢杆菌和短芽孢杆菌等主要寄主，重点介绍了细胞外异源蛋白的生产。在此，我们重点关注分泌途径，如Sec、双精氨酸易位（Tat）途径和ATP结合盒（ABC）转运体系统，它们控制蛋白质跨膜输出。从启动子优化和密码子适应到信号肽工程、菌株修饰以及蛋白质融合技术的分子策略已经得到强调，从而提高了分泌效率和产物产量。本文综述了革兰氏阳性和革兰氏阴性系统的比较见解，以及案例研究，以及针对不同宿主和蛋白质量身定制方法的重要性。这篇综述进一步讨论了基于CRISPR/ cas9n的遗传干预的日益增长的相关性以及微生物生理学在促进优化蛋白质折叠和运输中的作用。总之，合成生物学、分泌工程和宿主优化的整合为推进高价值重组蛋白的细胞外生产提供了有希望的途径。

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引用次数: 0

Optimizing extraction of antioxidant-enriched Dillenia ovata stem bark and evaluating its antidiabetic activities in vitro and in vivo 富抗氧化剂的枸杞茎皮提取工艺优化及体内外抗糖尿病活性评价

IF 4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Current Research in Biotechnology

Pub Date : 2025-01-01 DOI: 10.1016/j.crbiot.2025.100348

Trang Thi Xuan Dai , Tran Chi Linh , Chong Kim Thien Duc , Nguyen Trong Tuan

Purposes

Our objectives included optimizing extraction of antioxidant-enriched Dillenia ovata stem bark (DOSB), and testing the antidiabetic effects of optimal extract.

Methods

This study employed a Box–Behnken design-based statistical model to enhance the extraction of antioxidant-enriched compounds from Dillenia ovata stem bark. In vitro antidiabetic activity was assessed by inhibiting the key enzymes α-amylase and α-glucosidase, and in vivo testing was conducted using an alloxan-induced diabetic mice model.

Results

The optimal extraction conditions were 28.43 °C, 25.48 h, 72.80 % ethanol, and a material-to-solvent ratio of 1:10 (w/v). Two compounds, betulinaldehyde and betulinic acid, were isolated for the first time from the ethyl acetate fraction of the optimal DOSB extract. The IC₅₀ values of the optimal extract against α-amylase and α-glucosidase were 18.64 µg/mL and 10.26 µg/mL, respectively, whereas the two isolated compounds also exhibited considerable inhibitory activities. In vivo, the optimized DOSB extract at a dose of 200 mg/kg BW demonstrated comparable or superior therapeutic effects to the non-optimized extract at 400 mg/kg BW, suggesting improved potency resulting from the optimized extraction process. The DOSB extract reduced blood glucose levels, promoted weight loss, showed antioxidant activity by decreasing malondialdehyde (MDA), increased glutathione (GSH) in liver tissue, restored pancreatic structure, and normalized blood lipid profiles.

Conclusion

The study supports the continued use of Dillenia ovata stem bark in diabetes management and encourages conservation efforts to ensure its sustainable use.

目的：优化富含抗氧化剂的枸杞子茎皮（DOSB）的提取工艺，并考察其抗糖尿病作用。方法采用基于Box-Behnken设计的统计模型，对枸杞子茎皮中富含抗氧化物质的提取进行强化。通过抑制关键酶α-淀粉酶和α-葡萄糖苷酶来评估其体外抗糖尿病活性，并采用四氧嘧啶诱导的糖尿病小鼠模型进行体内实验。结果最佳提取条件为28.43℃，25.48 h， 72.80%乙醇，料液比1:10 （w/v）。从最佳DOSB提取物的乙酸乙酯部分首次分离到两个化合物，分别为白桦醛和白桦酸。最优提取物对α-淀粉酶和α-葡萄糖苷酶的IC50值分别为18.64µg/mL和10.26µg/mL，且两种化合物均表现出较强的抑制活性。在体内实验中，优化后的DOSB提取物在200 mg/kg BW剂量下的治疗效果与未优化的400 mg/kg BW剂量相当或更好，表明优化后的提取工艺提高了药效。DOSB提取物降低血糖水平，促进体重减轻，通过降低丙二醛（MDA），增加肝组织中的谷胱甘肽（GSH），恢复胰腺结构和正常化血脂显示抗氧化活性。结论本研究支持在糖尿病防治中继续使用水莲茎皮，并鼓励保护工作以确保其可持续利用。

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引用次数: 0

Exploring the innovation landscape of ginger: Insights from patent documentation analysis 探索生姜的创新前景：来自专利文件分析的见解

IF 3.6 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Current Research in Biotechnology

Pub Date : 2025-01-01 DOI: 10.1016/j.crbiot.2025.100299

Maima Matin , Bhupinder Kapoor , Monica Gulati , Michel-Edwar Mickael , Farhan Bin Matin , Agnieszka Kamińska , Joanna Bensz , Dariusz Sołdacki , Agnieszka Wierzbicka , Monika Marcinkowska-Lesiak , Ahmed Fatimi , Hong-Yan Liu , Ren-You Gan , Olga Adamska , Artur Stolarczyk , Jarosław Olav Horbańczuk , Artur Jóźwik , Atanas G. Atanasov

Zingiber officinale, commonly known as ginger, holds a prominent place in both culinary and medicinal practices worldwide. This study is the first to present a comprehensive analysis of ginger-related patents, aiming to elucidate patterns and trends in innovation surrounding this versatile plant. The analysis, conducted using The Lens database, encompasses 7193 patent documents retrieved on April 26, 2025. Annual patent counts illustrate a significant increase in ginger-related patents over the years, with a notable rise observed since 2010. Analysis of the Cooperative Patent Classification system provides a structured framework for organizing these patents, revealing predominant covered categories such as food compositions, plant extracts, and tea substitutes. Additionally, the top-cited patents underscore the diverse applications of ginger, ranging from therapeutic formulations to food and beverage additives. Furthermore, examination of frequently mentioned diseases in ginger patents identifies respiratory ailments, digestive disorders, and skin conditions as key therapeutic areas. These findings show the multifaceted nature of ginger’s pharmacological properties and its potential in addressing various health challenges. Overall, by focusing on ginger as a key food and medicine plant, this study highlights the significance of patent analysis in understanding the innovation landscape of medicinal plants and nutraceuticals, and is specifically opening the ways for future research and development in ginger utilization for global health promotion and sustainable development.

姜，俗称姜，在世界各地的烹饪和医学实践中都占有重要地位。本研究首次对生姜相关专利进行了全面分析，旨在阐明围绕这种多功能植物的创新模式和趋势。该分析是使用The Lens数据库进行的，包含了2025年4月26日检索到的7193份专利文件。年度专利数量表明，多年来与生姜相关的专利数量显著增加，自2010年以来出现了显著增长。对合作专利分类系统的分析为组织这些专利提供了一个结构化的框架，揭示了主要涵盖的类别，如食品成分、植物提取物和茶叶替代品。此外，被引用最多的专利强调了生姜的多种应用，从治疗配方到食品和饮料添加剂。此外，对生姜专利中经常提到的疾病进行检查，发现呼吸系统疾病、消化系统疾病和皮肤疾病是关键的治疗领域。这些发现显示了生姜药理特性的多面性及其在解决各种健康挑战方面的潜力。总体而言，本研究以生姜为重点食药植物，突出了专利分析对了解药用植物和保健品创新格局的重要意义，为未来生姜利用的研究开发开辟了道路，促进全球健康和可持续发展。

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引用次数: 0

Marine-derived secondary metabolites in oncology: A comprehensive review 肿瘤中的海洋次生代谢物：综合综述

IF 3.6 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Current Research in Biotechnology

Pub Date : 2025-01-01 DOI: 10.1016/j.crbiot.2025.100300

Samah S. Abuzahrah , Serag Eldin I. Elbehairi , Tahani Bakhsh , Ahmed Atwa , Nouf Juaid , Reham Hassan Mekky

Marine-derived secondary metabolites are emerging as promising anticancer agents due to their structural diversity and potent bioactivity. Within the scope of this all-encompassing study, the most recent advancements in the identification, characterization, and therapeutic applications of marine-derived compounds in oncology are studied. In this review, we discuss various types of bioactive metabolites that are significant, such as macrolactins, polyketides, terpenes, and peptides that are derived from diverse marine organisms viz., bacteria, fungi, actinomycetes, microalgae, macroalgae, mangroves, sponges, coral reefs, mollusks, and echinodermata Unique compounds from marine organisms exhibit diverse mechanisms that selectively target cancer cells. This minimizes harm to healthy tissues and reduces side effects.“These metabolites interfere with key cancer progression pathways such as immune modulation, apoptosis, angiogenesis, metastasis, and cell cycle regulation. Additionally, they enhance conventional treatments like chemotherapy and immune therapy by overcoming drug resistance, particularly multidrug resistance (MDR) and the persistence of cancer stem cells (CSCs), major contributors to therapy failure. CSCs, characterized by self-renewal and therapy resistance, play a central role in tumor recurrence and drug resistance. This review highlights the novelty of marine metabolites, providing a comprehensive inventory of their clinical and commercial applications while emphasizing their role in advancing green medicine through sustainable drug discovery practices. This review shows the promise of marine-derived secondary metabolites in building the future of cancer therapies by incorporating insights from current studies. It also inspires further exploration of the development of these metabolites as clinically practicable treatments.

海洋次生代谢物由于其结构的多样性和强大的生物活性，正成为一种有前景的抗癌药物。在这个包罗万象的研究范围内，研究了海洋衍生化合物在肿瘤鉴定、表征和治疗应用方面的最新进展。在这篇综述中，我们讨论了各种重要的生物活性代谢物，如巨乳素、聚酮、萜烯和肽，它们来自不同的海洋生物，如细菌、真菌、放线菌、微藻、大藻、红树林、海绵、珊瑚礁、软体动物和棘皮动物。这样可以最大限度地减少对健康组织的伤害并减少副作用。“这些代谢物干扰关键的癌症进展途径，如免疫调节、细胞凋亡、血管生成、转移和细胞周期调节。此外，它们通过克服耐药性，特别是多药耐药（MDR）和癌症干细胞（CSCs）的持久性来增强化疗和免疫治疗等传统治疗，这是治疗失败的主要原因。CSCs具有自我更新和耐药的特点，在肿瘤复发和耐药中起核心作用。这篇综述强调了海洋代谢物的新颖性，提供了其临床和商业应用的全面清单，同时强调了它们在通过可持续的药物发现实践推进绿色医学方面的作用。这篇综述表明，通过结合当前研究的见解，海洋衍生的次级代谢物在建立癌症治疗的未来方面具有很大的前景。这也激发了进一步探索这些代谢物的发展作为临床可行的治疗方法。

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引用次数: 0

Phylogenetically distinct fungal expansins show different binding preferences towards cellulosic materials and enhance cellulase activity 系统发育上不同的真菌扩张蛋白对纤维素材料表现出不同的结合偏好，并增强纤维素酶的活性

IF 3.6 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Current Research in Biotechnology

Pub Date : 2025-01-01 DOI: 10.1016/j.crbiot.2025.100296

Taru Koitto , Deepika Dahiya , Martina Huusela , Merja Penttilä , Emma Master

Expansins and expansin-like proteins are found in plants and microbes, and can disrupt the cellulosic microfibril network of plant cell walls. While plant expansins play a role in cell wall formation, microbial expansin-like proteins reportedly enhance the activity of lignocellulolytic enzymes. Herein, two novel fungal expansin-like proteins, AmaEXLX1 from Allomyces macrogynus and ApuEXLX1 from Aureobasidium pullulans, were recombinantly produced in Pichia pastoris. While both AmaEXLX1 and ApuEXLX1 retain typical expansin structure, they share low sequence identity (22.5 %) and different predicted pI values (5.8 and 8.8, respectively), which was expected to impact their action on cellulosic substrates. Accordingly, adsorption of the proteins on cellulose nanofibrils (CNF) and the impact of the expansin-like proteins on the hydrolysis of CNF by an endoglucanase (Cel7B) was investigated using quartz crystal microbalance with dissipation (QCM-D). AmaEXLX1 showed higher affinity towards cellulose compared to ApuEXLX1, which was correlated to missing key aromatic residues in the polysaccharide binding surface of ApuEXLX1. The pretreatment of a CNF film with AmaEXLX1 and ApuEXLX1 increased the initial rate of Cel7B activity. This study underscores similarities between the impacts that bacterial and fungal expansin-like proteins can have on the enzymatic deconstruction of cellulose, and sequence properties that could impact expansin performance.

扩张蛋白和扩张蛋白样蛋白存在于植物和微生物中，可以破坏植物细胞壁的纤维素微纤维网络。虽然植物膨胀素在细胞壁形成中起作用，但据报道微生物膨胀素样蛋白增强了木质纤维素水解酶的活性。本研究在毕赤酵母中重组产生了两个新的真菌扩张蛋白样蛋白，分别是来自巨绿异酵母菌（alloomyces macrogynus）的AmaEXLX1和来自pululans的auobasidium ApuEXLX1。虽然AmaEXLX1和ApuEXLX1都保留了典型的扩展蛋白结构，但它们具有较低的序列同一性（22.5%）和不同的预测pI值（分别为5.8和8.8），这预计会影响它们对纤维素底物的作用。因此，利用石英晶体耗散微天平（QCM-D）研究了蛋白质在纤维素纳米原纤维（CNF）上的吸附以及膨胀蛋白样蛋白质对内切葡聚糖酶（Cel7B）水解CNF的影响。与ApuEXLX1相比，AmaEXLX1对纤维素的亲和力更高，这与ApuEXLX1的多糖结合表面缺失关键芳香残基有关。用AmaEXLX1和ApuEXLX1预处理CNF膜提高了Cel7B的初始活性率。这项研究强调了细菌和真菌类扩张蛋白对纤维素酶解的影响之间的相似性，以及可能影响扩张蛋白性能的序列特性。

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引用次数: 0

Corrigendum to “Favipiravir vs. Deferiprone: tautomeric, photophysical, in vitro biological studies, and binding interactions with SARS-Cov-2-MPro/ACE2” [Curr. Res. Biotechnol. 7 (2024) 100176] “Favipiravir与去铁素：互变异构体、光物理、体外生物学研究以及与SARS-Cov-2-MPro/ACE2的结合相互作用”的勘误表。Res. biotechnology . 7 (2024) 100176 [j]

IF 3.6 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Current Research in Biotechnology

Pub Date : 2025-01-01 DOI: 10.1016/j.crbiot.2025.100286

Nikolay T. Tzvetkov , Martina I. Peeva , Maya G. Georgieva , Vera Deneva , Aneliya A. Balacheva , Ivan P. Bogdanov , Maria Ponticelli , Luigi Milella , Kiril Kirilov , Maima Matin , Hans-Georg Stammler , Atanas G. Atanasov , Liudmil Antonov

引用次数: 0

Termitomyces mushroom extract-mediated synthesis of silver nanoparticles and its in-vitro activity against drug-resistant Candida species 白蚁菌蘑菇提取物介导的纳米银合成及其对耐药念珠菌的体外活性研究

IF 3.6 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Current Research in Biotechnology

Pub Date : 2025-01-01 DOI: 10.1016/j.crbiot.2025.100279

Naheem Adekilekun Tijani , Joseph Hokello , Emmanuel Eilu , Saheed Adekunle Akinola , Abdullateef Opeyemi Afolabi , Ibrahim Ntulume , Ismail Abiola Adebayo

Green nanotechnology has continued to gain popularity as a novel and alternative strategy to overcome the menace caused by drug-resistant pathogens. For the first time, this study explores an efficient, eco-friendly, and economical approach for the mycogenic synthesis of silver nanoparticles (AgNPs) by utilizing the aqueous extract of wild Termitomyces species of edible mushroom. The mushroom-assisted AgNPs synthesis was validated with visual colour observation and characterized with UV–Vis spectrophotometer, SEM, EDX, XRD, FTIR and DLS. The potential anticandidal efficacy of the synthesized AgNPs was investigated against six clinical isolates of resistant pathogenic Candida species. A typical Ag surface plasmon resonance (SPR) had absorbance maxima wavelength within 371–404 nm range, with a spherical shape particulate structure in the size range of 28 to 45 nm according to UV–Vis and SEM analyses respectively. Remarkable antifungal activity was recorded against a good number of the Candida isolates with MICs values in the range of 0.0122–0.0976 mg/mL. We conclude that wild Termitomyces mushroom is a suitable biomaterial for AgNPs synthesis and an effective antifungal agent which could be adopted as a novel therapeutic agent for efficient management of drug-resistant Candida pathogens.

绿色纳米技术作为克服耐药病原体造成的威胁的一种新的替代策略继续受到欢迎。本研究首次探索了一种高效、环保、经济的方法，利用野生食用菌白蚁的水萃取物合成银纳米颗粒（AgNPs）。用肉眼颜色观察验证了蘑菇辅助AgNPs的合成，并用紫外可见分光光度计、SEM、EDX、XRD、FTIR和DLS对其进行了表征。研究了合成的AgNPs对6株耐药致病性念珠菌临床分离株的潜在抗药效果。典型银表面等离子体共振（SPR）的最大吸光度波长在371 ~ 404 nm范围内，紫外可见光谱和扫描电镜显示SPR为28 ~ 45 nm的球形颗粒结构。对大量念珠菌分离株具有显著的抑菌活性，mic值在0.0122 ~ 0.0976 mg/mL之间。我们认为，野生白蚁菌是一种适合合成AgNPs的生物材料，是一种有效的抗真菌药物，可以作为一种新的治疗药物，有效地管理耐药念珠菌病原体。

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引用次数: 0