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Functional Characterisation of a Calmodulin-Binding Receptor-Like Cytoplasmic Kinase (GmCBRLCK1) in Glycine max (L.) Merr. using Bioinformatic Tools 甘氨酸max (L.)中钙调素结合受体样细胞质激酶(GmCBRLCK1)的功能特征稳定。使用生物信息学工具
Pub Date : 2019-05-24 DOI: 10.21467/IAS.7.1.38-47
E. Bobo, Pias Munosiyei, Percy Jinga, E. Zingoni
An understanding of the function of signaling genes/proteins in soybean is vital for comprehending plant growth and development. The objective of this study was to functionally characterize a calmodulin-binding receptor-like cytoplasmic kinase gene (Glyma.13G161700) from Glycine max. Bioinformatic analyses were performed for the characterization. Expression profile of GmCBRLCK1 gene in soybean tissue was assessed using Genevisible. Functional genomic analysis for gene expression regulation and co-expression analysis was evaluated using micro array data from Affymetrix Soybean Genome Array platform in GENEVESTIGATOR v3. Gene ontology functional predictions were determined through FFPred 2.0. The results showed that the calmodulin-binding receptor-like cytoplasmic kinase gene is predominantly expressed in the pericycle and syncytium in root seedlings and in the palisade cells of the legume. The gene was shown to be highly upregulated in response to root exposure to Phytophthora sojae, Heterodera glycines and aluminium stress. Co-expressed genes during the legume development showed Pearson’s correlation co-efficient of 1 to Glyma.13G161700. Gene ontology predictions confirmed the signaling and metabolic functions of the kinase gene and its primary locations are the membrane and endomembrane system of G. max. The study therefore suggests that Glycine max calmodulin-binding receptor-like cytoplasmic kinase (GmCBRLCK1) is involved in receptor signaling pathways to enhance seedling tolerance to root infection by P. sojae, H. glycines, and to aluminium stress. The kinase gene is also involved in regulation of metabolic processes that aid in growth and development of soybean seedling.
了解大豆信号基因/蛋白的功能,对了解植物的生长发育具有重要意义。本研究的目的是对Glycine max中钙调素结合受体样细胞质激酶基因(Glyma.13G161700)进行功能表征。生物信息学分析进行表征。应用genevvisible软件分析GmCBRLCK1基因在大豆组织中的表达谱。基因表达调控和共表达分析的功能基因组分析使用genevetiator v3中Affymetrix大豆基因组阵列平台的微阵列数据进行评估。通过FFPred 2.0进行基因本体功能预测。结果表明,钙调素结合受体样细胞质激酶基因在根苗中柱鞘和合胞体以及豆科植物栅栏细胞中主要表达。该基因在根暴露于大豆疫霉、甘氨酸异源霉和铝胁迫下表现出高度上调。豆科植物发育过程中共表达基因的Pearson相关系数为1 ~ Glyma.13G161700。基因本体论预测证实了激酶基因的信号和代谢功能,其主要位置是g.m ax的膜和内膜系统。因此,研究表明甘氨酸max钙调素结合受体样细胞质激酶(GmCBRLCK1)参与了受体信号通路,增强了幼苗对大豆、甘氨酸根侵染和铝胁迫的耐受性。该激酶基因还参与调节大豆幼苗生长发育的代谢过程。
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引用次数: 1
Factors Influencing Midwifery Clinical Decision-making 影响助产临床决策的因素
Pub Date : 2019-05-10 DOI: 10.21467/IAS.7.1.28-32
Betty Sakala
Clinical decision-making is an important element in midwifery practice. Midwives are required to have a sound knowledge to manage complications during childbirth. Any misjudgement by a midwife may lead to adverse birth outcomes. The aim of this paper is to review factors that contribute to clinical decision-making of midwives. This was achieved by reviewing published research articles. Studies have shown that shortage of human and material resources, poor skill mix, absence of mentors and lack of autonomy are some of the contributing factors that may affect midwives’ decision-making.
临床决策是助产实践的重要组成部分。助产士必须具备处理分娩并发症的良好知识。助产士的任何错误判断都可能导致不良的分娩结果。本文的目的是审查的因素,有助于助产士的临床决策。这是通过审查已发表的研究文章来实现的。研究表明,人力和物质资源短缺、技能组合差、缺乏导师和缺乏自主权是可能影响助产士决策的一些因素。
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引用次数: 2
Toxicological Safety Assessment of Molluscicides Against Non-target Aquatic Biota; Colisa fasciatus 杀螺剂对非目标水生生物的毒理学安全性评价Colisa fasciatus
Pub Date : 2019-04-30 DOI: 10.21467/IAS.7.1.21-27
N. Soni, V. Singh
Plants Terminalia arjuna and Tamarindus indica are known to have a significant molluscicidal potential to control the population of vector snails. Since the molluscicides are considered an emerging pollutant and are frequently detected in surface water bodies and found to be a great threat for aquatic biota. Hence, the main objective of this research is to critically evaluate the ectotoxicological and chronic effect of plant-derived molluscicides on other aquatic biotas. With these references this study deals with the safety measurement of molluscicides arjnolic acid, saponin and procynadine from T. arjuna and T. Indica against the fish Colisa fasciatus which share the same habitat with snails. The result of toxicity experiment reveals that fishes showed no mortality against 24h LC90 (against L. acuminata and I. exustus) up to 96h exposure duration. The enzyme bioassays of these molluscicides on the nervous tissue of fish showed no significant effect on key enzymes Acetylcholinesterase, acid and alkaline phosphates activity in comparison to control group of fishes. These results indicated that the application of arjunolic acid, saponin, and procynadine derive from plant T. arjuna and T. indica at its maximum concentration (24h LC90 of L. acuminata and I. exustus) and exposure duration (96h) did not cause any mortality or treatment-related enzymes inactivity in fishes. The study conclusively proved the ecotoxicological and chronic safety of plant-derived molluscicides arjunolic acid, saponin, and procynadine on non-target animals in the aquatic environment.
据了解,铁蒺藜和柽柳在控制病媒蜗牛种群方面具有显著的杀螺潜力。由于杀软体剂被认为是一种新兴的污染物,经常在地表水中被检测到,并被发现对水生生物群构成了巨大的威胁。因此,本研究的主要目的是批判性地评估植物源性杀螺剂对其他水生生物的生态毒理学和慢性效应。在此基础上,本文研究了阿朱那和印度赤藓中杀螺剂arjnolic acid,皂苷和procynadine对与蜗牛共享栖息地的Colisa fasciatus的安全性测定。毒力实验结果表明,暴露时间长达96h的LC90(对acuminata和exustus的LC90)对鱼类无致死作用。结果表明,与对照组相比,这些杀螺剂对鱼神经组织关键酶乙酰胆碱酯酶、酸性和碱性磷酸盐活性无显著影响。这些结果表明,在最大浓度(L. acuminata和I. exustus的24h LC90)和暴露时间(96h)下,来自植物arjuna和T. indica的arjunolic酸、皂苷和procynadine的施用不会导致鱼类死亡或与处理相关的酶活性降低。本研究最终证明了植物源性杀软体剂arjunolic acid、皂苷和procynadine对水生环境中非靶动物的生态毒理学和慢性安全性。
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引用次数: 1
Symptom Severity of Nicotiana benthamiana Plants Inoculated with Agrobacterium Containing Infectious DNA-A Clones of Honeysuckle Yellow Vein Virus (HYVV) 含金银花黄脉病毒(HYVV)感染性DNA-A克隆农杆菌接种本烟植株的症状严重程度
Pub Date : 2019-04-17 DOI: 10.21467/IAS.7.1.12-20
Sung Oh, C. Choi
To investigate the pathogenicity and virulence of the Honeysuckle yellow vein virus (HYVV) lacking betasatellites, PCR amplified unit-lengths of DNA-A genome of HYVV-[DJ] were cloned into binary vector pRI101-AN, and generated HYVV-[DJ]-1mer, -1.3mer and -2mer genomes. Each construct was transformed into Agrobacterium cells and agro-inoculated into young leaves of Nicotiana benthamiana. Except for the HYVV-[DJ]-1mer, HYVV-[DJ]-1.3mer and -2mer clones caused pronounced disease symptoms in N. benthamiana. HYVV-[DJ]-2mer agro-inoculated plants showed more severe plant stunting with downward leaf curling and crinkling than those of HYVV-[DJ]-1.3mer agro-inoculated plants. To discriminate the clone’s virulence quantitatively, SYBR Green-based real-time PCR was performed for the quantification of the target virulence gene DNA in agro-inoculated plants that were collected at weekly intervals for 4 weeks. Regression analysis was obtained from the standard curves by plotting Ct values over the logarithm of the amount of V1 protein gene DNA present in a dilution series of plasmid containing the full-length HYVV-[DJ] genome. Equation of the HYVV V1 DNA standard curve was used to quantify V1 gene DNA concentration in agro-inoculated plants with each clone. The accumulation of V1 gene DNA in HYVV-[DJ]-1.3mer agro-inoculated plants reached the peak level at 4 weeks post inoculation, while the accumulation of V1 gene DNA in HYVV-[DJ]-2mer agro-inoculated plants reached the peak level at 3 weeks post inoculation. The amount of V1 DNA in HYVV-[DJ]-1.3mer agro-inoculated plants was significantly more than that in HYVV-[DJ]-2mer agro-inoculated plants. Considering the results, there was a difference between the accumulation of virus DNA and the symptom severity of the analyzed plants agro-inoculated with each clone. It suggested that the infectious clones’ virulence is not necessarily correlated with the symptom severity.
为了研究缺乏betasat卫星的金银花黄脉病毒(HYVV)的致病性和毒力,将PCR扩增的HYVV-[DJ] DNA-A基因组单位长度克隆到二元载体pRI101-AN中,得到HYVV-[DJ]-1mer、-1.3mer和-2mer基因组。将每个构建体转化为农杆菌细胞,并接种到烟叶中。除HYVV-[DJ]-1mer外,HYVV-[DJ]-1.3mer和-2mer克隆在benthamiana中引起明显的疾病症状。与HYVV-[DJ]-1.3mer农业接种植株相比,HYVV-[DJ]-2mer农业接种植株发育迟缓严重,叶片向下卷曲、起皱。为了定量鉴定该克隆的毒力,采用SYBR green实时荧光定量PCR技术对每隔一周采集的农业接种植株的靶毒力基因DNA进行定量。通过绘制含有HYVV-[DJ]全长基因组的稀释质粒系列中V1蛋白基因DNA数量的对数上的Ct值,从标准曲线获得回归分析。利用HYVV V1 DNA标准曲线方程,定量测定各无性系农业接种植株中V1基因DNA浓度。HYVV-[DJ]-1.3mer农业接种植株的V1基因DNA积累量在接种后4周达到峰值,而HYVV-[DJ]-2mer农业接种植株的V1基因DNA积累量在接种后3周达到峰值。HYVV-[DJ]-1.3mer农业接种植株的V1 DNA含量显著高于HYVV-[DJ]-2mer农业接种植株。从结果来看,每个克隆接种的分析植物的病毒DNA积累量和症状严重程度存在差异。提示传染性克隆的毒力与症状的严重程度并不一定相关。
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引用次数: 0
Physiochemical Analysis of Sandalwood (Santalum Album) Leaf Litters Degraded by Eisenia foetida and Eudrilus eugenia 紫檀木(Santalum Album)叶片凋落物的理化分析(英文
Pub Date : 2019-02-10 DOI: 10.21467/IAS.7.1.6-11
P. Bhati, Ritu Nagar, A. Titov
The decay of leaf litter by microflora and fauna furnish nutrient supply to the soil and also uphold ecological sustainability. Applying of proper technique and exploring of result provides information for the betterment of agricultural system. Vermicomposting of Sandalwood (Santalum album) leaf litters were studied with an emphasis of physio-chemical deviation during the process and also compared with 100 % cattle dung. Obtained result explore that temperate of 50 % leaf litter (LL) and 100 % cattle dung (CD) was slightly elevated (37ºC ±1 ºC and 35ºC ±1 ºC respectively) at beginning phase and later came down to ambient level (20ºC±1 ºC). The total organic carbon (TOC) exhausted 44 % in 50 % LL Vermicomposting mixture while 70 % in 100 % CD during the process. At the final stage, TOC found more in 50% LL as compared to 100% CD. Nitrogen content was found 1.02±0.1 in 50 % LL and 0.88±0.1 in 100 % CD at the initial phase but after completion of Vermicomposting, their level was increased up to 40 to 44 %.  pH was also measured during vermicomposting and found 7.2±0.1 in 50% LL while 8.4±0.1 in 100% CD at the initial phase. The at the end of process pH raised and set up to 8.2 ±0.1 in 50% LL while in 100% CD it was found 8.0 ±0.1.
微生物和动物对凋落叶的腐烂为土壤提供养分,也维持了生态的可持续性。适当技术的应用和效果的探索为农业系统的改进提供了信息。以紫檀叶凋落物为研究对象,重点研究了蚯蚓堆肥过程中的理化偏差,并与100%牛粪进行了比较。所得结果探讨了50%凋落叶(LL)和100%牛粪(CD)的温度在初始阶段略有升高(分别为37℃±1℃和35℃±1℃),随后降至环境水平(20℃±1℃)。在此过程中,50% LL蚯蚓堆肥的总有机碳(TOC)排放量为44%,而100% CD的总有机碳排放量为70%。在最后阶段,50% LL的TOC含量高于100% CD。初始阶段,50% LL的氮含量为1.02±0.1,100% CD的氮含量为0.88±0.1,但在完成蚯蚓堆肥后,它们的含量增加到40 ~ 44%。在蚯蚓堆肥过程中,测定了初始阶段的pH值,50% LL为7.2±0.1,100% CD为8.4±0.1。处理结束pH值在50%乳酸液中升高至8.2±0.1,在100%乳酸液中升高至8.0±0.1。
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引用次数: 0
Comparative Proximate Composition of Maize (Zea mays L.) Varieties Grown in South-western Nigeria 尼日利亚西南部种植的玉米(Zea mays L.)品种的近似成分比较
Pub Date : 2019-01-27 DOI: 10.21467/IAS.7.1.1-5
O. Adeniyi, O. Ariwoola
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引用次数: 14
Behavioral tendencies of single parent students 单亲学生的行为倾向
Pub Date : 2018-11-30 DOI: 10.26697/ijsa.2018.1-2.03
H. Usakli
Background and Aim of Study: A Family is social unit of two or more people related by blood, marriage, or adoption and having a shared commitment to the mutual relationship. The definition of single parent is someone who has a child or children but no husband, wife, or partner who lives with them. Death of a partner and divorce are main causes of being single parent. Children are affected by divorce in many different ways, varying by the circumstances and age of the child. Children whose ages are seven to twelve are much better at expressing emotions and accepting parentage breakage, but often distrust their parents, rely on outside help and support for encouragement, and may manifest social and academic problems. The aim of the study: to find out teachers opinion of single parents’ students’ behavioral tendency. Material and Methods: This qualitative study represents 30 teachers’ opinions on single parent students’ problems. Results: Not only in deep theoretical framework but also recent studies underline the importance of healthy family relation on child wellbeing. Every child may have potential for single parent in nowadays society. Experienced elementary teachers claim that single parent students are more submissive and aggressive. In addition of this, they are less assertive when comparing to their two parents counterparts. Conclusions: Not only school psychologists and guidance practitioners but also teachers and school principles should be aware of the potential single parent students’ needs. Being more assertive or aggressive creates fewer opportunity for single parent students. Productive society will be raised with only equal sublimation of all children’s developments.
背景和研究目的:家庭是由两个或两个以上的人组成的社会单位,他们有血缘、婚姻或收养关系,并对彼此的关系有共同的承诺。单亲父母的定义是有一个或多个孩子,但没有丈夫、妻子或伴侣与他们生活在一起的人。配偶死亡和离婚是单身父母的主要原因。离婚对孩子的影响有许多不同的方面,因环境和孩子的年龄而异。7到12岁的孩子更善于表达情感和接受父母关系的破裂,但往往不信任父母,依赖外界的帮助和支持来鼓励,并可能表现出社会和学业上的问题。本研究的目的:了解教师对单亲家庭学生行为倾向的看法。材料与方法:本定性研究代表了30位教师对单亲学生问题的看法。结果:无论是在深刻的理论框架,还是最近的研究都强调了健康的家庭关系对儿童幸福的重要性。在当今社会,每个孩子都有成为单亲父母的潜力。有经验的小学教师声称,单亲家庭的学生更顺从,更有攻击性。除此之外,与双亲相比,他们不那么自信。结论:单亲学生的潜在需求不仅需要学校心理学家和指导工作者的关注,还需要教师和学校负责人的关注。更加自信或咄咄逼人给单亲学生创造的机会更少。只有在所有儿童的发展都得到同等的升华的情况下,生产性社会才会建立起来。
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引用次数: 6
Verification of the system of preparation of future specialists of the social sphere for the prevention of maladjustment of pupils in various social institutions 核查培养未来社会领域专家的制度,以防止学生在各种社会机构中适应不良
Pub Date : 2018-11-30 DOI: 10.26697/IJSA.2018.1-2.02
V. Kostina
Background and Aim of Study: The article is devoted to the problem of professional preparation of specialists in the social sphere. The aim of the study: to describe the structure of conducting a pedagogical experiment on the research problem and determine its qualitative results. Material and Methods: During the implementation of the pilot study to determine the effectiveness of the the impact, made by developed system of training future specialists in the social sphere to prevent the maladjustment of pupils, on their professional competence, methods of comparing, analyzing and generalizing the results of the process of professional education in the control and experimental groups were used. Results: The logical structure of conducting an experimental research on the training of future social pedagogues and social workers for the prevention of maladjustment of pupils, which envisaged the implementation of five consecutive stages and methodological support for its implementation, as well as a system of criteria (motivational and value-based, affective-conative, cognitive-instrumental and professional activity competencies) and corresponding indicators (interest and need of prevention of maladjustment of pupils; professional worldview as the basis for the development of professional culture, characteristics of temperament and will, that are ensuring the successful implementation of prevention of maladjustment of pupils; personal qualities necessary for working with vulnerable contingents, general-pedagogical potential, developed social intelligence, knowledge of the organization of socio-pedagogical and social activities of prevention of maladjustment of pupils in various social institutions; knowledge of the technological basis for the prevention of maladjustment of pupils; professional knowledge and skills in the prevention of maladjustment of pupils; experience in social-pedagogical and social preventive work). Conclusions: Qualitative changes in personal and professional characteristics of future specialists of the social sphere are determined in accordance with the presented criteria system after the implementation of the forming part of the study.
研究背景和目的:本文致力于社会领域专家的职业准备问题。本研究的目的:描述对研究问题进行教学实验的结构,并确定其定性结果。材料和方法:在试点研究的实施过程中,为了确定发达的社会领域未来专家培训系统对其专业能力的影响的有效性,以防止学生的不适应,在对照组和实验组中使用了比较,分析和概括专业教育过程结果的方法。结果:进行一项关于培训未来社会教师和社会工作者以预防学生适应不良的实验研究的逻辑结构,该研究设想实施五个连续阶段,并为其实施提供方法上的支持,以及一套标准系统(动机和基于价值的、情感的、认知工具和专业活动能力)和相应的指标(预防学生适应不良的兴趣和需要;专业世界观作为发展专业文化、气质和意志的基础,是学生适应不良预防成功实施的保证;与弱势群体合作所必需的个人素质、一般教学潜力、发达的社会智力、组织社会-教学和社会活动的知识,以防止各种社会机构中的学生适应不良;了解预防小学生适应不良的技术基础;预防小学生适应不良的专业知识和技能;具有社会教育和社会预防工作经验)。结论:社会领域未来专家的个人和职业特征的质变是在研究形成部分实施后根据所提出的标准体系确定的。
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引用次数: 1
Training of future specialists in higher education institutions 培养高等教育机构未来的专业人才
Pub Date : 2018-11-30 DOI: 10.26697/ijsa.2018.1-2.01
Y. Melnyk, I. Pypenko
Background and Aim of Study: The research deals with studying issues concerning training of specialists in high school and the student’s role in this process. The perspective trends of training specialists in higher educational institutions are determined. They relate to such, where the university is a configuration part of much bigger establishments and processes and where it corresponds to the social and individual demands of the youth. The aim of the study: to ascertain competences, pedagogic technologies and methods, demanded by cadets and students, as well as to forecast perspective trends of studying in higher educational institutions. Material and Methods: A set of methods is used to study issues of training specialists in high school: collection of information, systematisation, rating assessment, analysis and results interpretation. The dispersion coefficient of Kendall concordance is calculated, its significance is proved on the basis of determining Pirson’s criterion for the significance level of 5% and 1%. The research was held in the academic years of 2013-2018 on the basis of National Academy of National Guard of Ukraine within the framework of the subject “University Education”. The average number of respondents was 535 people (35 groups), who studied at the Humanities Faculty, Technical Faculty, the Faculty of Economics and Management. Results: It is specified that there is a tendency of decreasing number of students who want to study in higher educational institutions. The demand of the student youth is determined for competences, pedagogic technologies and methods which are mostly required in high school. It is proposed to specify competences classification and content of the notions “professional competences” and “special competences”. Conclusions: On the basis of modern scientific and technical achievements, application of educational logistics and social demand, the main tendencies and future trends of training specialists in higher educational institutions in IT-sphere, technical, military, economic, medical and educational fields are forecast.
研究背景和目的:本研究涉及高中专家培训的研究问题以及学生在这一过程中的作用。确定了高等教育机构培养专家的前景趋势。它们与此相关,大学是更大的机构和流程的组成部分,它符合年轻人的社会和个人需求。本研究的目的是:确定学员和学生所需的能力、教学技术和方法,并预测在高等教育机构学习的前景趋势。材料与方法:采用信息收集、系统化、评分评估、分析和结果解释等一套方法对高中专科人才培养问题进行研究。计算了Kendall一致性的离散系数,并在确定5%和1%显著性水平的Pirson准则的基础上证明了其显著性。该研究于2013-2018学年在乌克兰国民警卫队国家学院的基础上,在“大学教育”主题的框架内进行。受访者的平均人数为535人(35组),他们就读于人文学院、技术学院、经济与管理学院。结果:研究表明,希望在高等教育机构学习的学生人数有减少的趋势。青年学生对能力、教学技术和方法的需求是由高中阶段最需要的。建议明确“专业能力”和“特殊能力”概念的能力分类和内容。结论:根据现代科技成果、教育后勤的应用和社会需求,预测了高等学校在信息技术、军事、经济、医疗、教育等领域培养人才的主要趋势和未来趋势。
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引用次数: 10
Еmpathy in the structure of psychological competence of the teacher of the higher educational institution Еmpathy在高校教师心理素质结构中的作用
Pub Date : 2018-11-30 DOI: 10.26697/IJSA.2018.1-2.04
S. Mitina
Background and Aim of Study: The efficiency of pedagogical activity depends not only of the level of professional knowledge and abilities of the teacher but also of the presence for him of the personality qualities necessary for optimal cooperation. One of such professionally-meaningful qualities of the teacher is the empathy able to do the process of education emotionally comfortable and productive. The aim of the study: to explore of level of empathy’s display of the teachers depending on experience of their pedagogical activity. Material and Methods: Theoretical (analysis and synthesis of the psychological literature); empirical (pilot study). 97 teachers of the higher educational institutions participated in the study; at the age of 27 to 62 years, with the work experience from 1 year to 30 years. Results. The results obtained indicate that the empathy is the important component in the structure of the professional competence of the teacher of the higher educational institution, and also that the empathic ability of the teacher is transformed with the development of life and professional experience. The empirical research shows the lowest rates of empathy among the teachers with the work experience of more than 25 years and among the young teachers with the work experience up to 10 years. Conclusions.It is concluded that there is the dependence of the empathy of professional experience, necessary to develop the empathy both at the stage of the training of the future teachers in the higher educational institutions and in the process of continuous professional education.
研究背景和目的:教学活动的效率不仅取决于教师的专业知识和能力水平,还取决于他是否具备最佳合作所需的人格素质。教师具有专业意义的品质之一是同理心,能够在教育过程中情绪舒适、富有成效。本研究的目的是:探讨教师在教学活动中的移情表现水平。材料和方法:理论(心理学文献的分析和综合);实证(试点研究)。97名高等教育机构的教师参与了研究;年龄27岁至62岁,具有1年至30年的工作经验。后果研究结果表明,移情是高等学校教师专业能力结构的重要组成部分,教师的移情能力随着生活和专业经验的发展而发生变化。实证研究表明,工作经验在25年以上的教师和工作经验在10年以下的年轻教师的移情率最低。结论:在高等学校培养未来教师的阶段和继续职业教育的过程中,存在着职业经验移情的依赖性,培养移情是必要的。
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引用次数: 0
期刊
International Journal of Science Annals
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