Pub Date : 2018-06-01DOI: 10.21608/jvmr.2017.43280
Nabila A. Ghazy, Wafaa R. Abd El-Aziz, Ibrahim, H.M., Shell W.S., Hosein H.I.
ARTICLE INFO Stability study of biological products especially living bacterial vaccines plays an important role for the determination of product changes in maintenance period, and ensures safety, efficacy and maintenance of biological properties of the vaccines. So, the objective of this study was to establish stability and keeping quality of the local Brucella melitensis Rev-1 vaccine using different types of stabilizers in lyophilization process. A long-term stability study was carried out for four batches of reduced-dose Brucella melitensis Rev-1 vaccine manufactured by veterinary serum and vaccine research institute using four different stabilizers. Stabilizers were: (A) sucrose and skimmed milk, (B and C) different concentrations of sucrose, sodium glutamate and gelatin, and (D) casein, sucrose and sodium glutamate. The quality control tests including colony forming unit, purity, dissociation and physicochemical tests on all batches until 12 months postproduction were performed. The obtained results indicated that in spite of collapse (shrinkage) of lyophilized cake in a number of bottles in batches prepared using stabilizer A, Brucella vaccine batches were stable and met the specification recommended by OIE 2012 for 12 months post-production in vaccine batches with stabilizers A and D. Article history:
{"title":"The use of different stabilizers for improving integrity of the locally prepared lyophilized Brucella melitensis Rev 1 vaccine","authors":"Nabila A. Ghazy, Wafaa R. Abd El-Aziz, Ibrahim, H.M., Shell W.S., Hosein H.I.","doi":"10.21608/jvmr.2017.43280","DOIUrl":"https://doi.org/10.21608/jvmr.2017.43280","url":null,"abstract":"ARTICLE INFO Stability study of biological products especially living bacterial vaccines plays an important role for the determination of product changes in maintenance period, and ensures safety, efficacy and maintenance of biological properties of the vaccines. So, the objective of this study was to establish stability and keeping quality of the local Brucella melitensis Rev-1 vaccine using different types of stabilizers in lyophilization process. A long-term stability study was carried out for four batches of reduced-dose Brucella melitensis Rev-1 vaccine manufactured by veterinary serum and vaccine research institute using four different stabilizers. Stabilizers were: (A) sucrose and skimmed milk, (B and C) different concentrations of sucrose, sodium glutamate and gelatin, and (D) casein, sucrose and sodium glutamate. The quality control tests including colony forming unit, purity, dissociation and physicochemical tests on all batches until 12 months postproduction were performed. The obtained results indicated that in spite of collapse (shrinkage) of lyophilized cake in a number of bottles in batches prepared using stabilizer A, Brucella vaccine batches were stable and met the specification recommended by OIE 2012 for 12 months post-production in vaccine batches with stabilizers A and D. Article history:","PeriodicalId":53046,"journal":{"name":"Journal of Veterinary Medical Research","volume":"58 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77215285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-06-01DOI: 10.21608/jvmr.2018.43299
Amira H. Mohamed, Kamal H.H., Walaa M.S. ahmed, Hanan E. Saeed
The current study was performed to evaluate the effect of acute and chronic hepatotoxicity induced by paracetamol and thioacetamide respectively on serum hormonal levels and biochemical parameters. Female Wistar albino rats were divided into 3 equal groups (C), (P) and (T). Group (C) were kept as control, group (P) were received paracetamol orally (500 mg/kg b.wt) daily for 15 days and those of group (T) were injected thioacetamide (200 mg/kg b.wt) intraperitonialy twice/ week for 90 days. In P group, results revealed significant elevation in liver enzyme activities (ALT, AST and ALP), T4, insulin (7 th day), estrogen (7 th and 15 th days), triglycerides (7 th day) and cholesterol levels throughout the experiment while serum proteins and T4 (15 th day) showed significant decreased values. Whereas, at 90 th days of chronic intoxicated group (T) resulted in significant elevation in liver enzyme activities (ALT, AST and ALP), bilirubin, estrogen, T4, triglycerides (60 th and 90 th days) and T3 (120 th day). While the levels of T4 and cortisol (60 th day), serum total protein, albumin, globulin (90 th day) and insulin (120 th day) showed significant decreased values when compared to control group. In conclusion, both paracetamol and thioacetamide cause different degrees of damage in liver of rats leading to clear changes in their hormonal and biochemical profiles. ARTICLE INFO
{"title":"Effect of liver diseases on hormonal and biochemical parameters in Wistar albino rat","authors":"Amira H. Mohamed, Kamal H.H., Walaa M.S. ahmed, Hanan E. Saeed","doi":"10.21608/jvmr.2018.43299","DOIUrl":"https://doi.org/10.21608/jvmr.2018.43299","url":null,"abstract":"The current study was performed to evaluate the effect of acute and chronic hepatotoxicity induced by paracetamol and thioacetamide respectively on serum hormonal levels and biochemical parameters. Female Wistar albino rats were divided into 3 equal groups (C), (P) and (T). Group (C) were kept as control, group (P) were received paracetamol orally (500 mg/kg b.wt) daily for 15 days and those of group (T) were injected thioacetamide (200 mg/kg b.wt) intraperitonialy twice/ week for 90 days. In P group, results revealed significant elevation in liver enzyme activities (ALT, AST and ALP), T4, insulin (7 th day), estrogen (7 th and 15 th days), triglycerides (7 th day) and cholesterol levels throughout the experiment while serum proteins and T4 (15 th day) showed significant decreased values. Whereas, at 90 th days of chronic intoxicated group (T) resulted in significant elevation in liver enzyme activities (ALT, AST and ALP), bilirubin, estrogen, T4, triglycerides (60 th and 90 th days) and T3 (120 th day). While the levels of T4 and cortisol (60 th day), serum total protein, albumin, globulin (90 th day) and insulin (120 th day) showed significant decreased values when compared to control group. In conclusion, both paracetamol and thioacetamide cause different degrees of damage in liver of rats leading to clear changes in their hormonal and biochemical profiles. ARTICLE INFO","PeriodicalId":53046,"journal":{"name":"Journal of Veterinary Medical Research","volume":"2016 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82812797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-06-01DOI: 10.21608/jvmr.2018.43304
Tamam S.M., E. N.M, Shokier K.A
lumpy skin disease virus (LSDV) was isolated, from naturally infected cattle that have a history of previous vaccination with live attenuated sheep pox virus (SPV) vaccine. The virus was isolated on chorio-allantoic membranes (CAM) of embryonated chicken eggs (ECE) and Madin Darby Bovine Kidney Cells (MDBK) and identified by agar gel precipitation test (AGPT) and immunofluorescent antibody technique (IFA). Characteristic pock lesions and intracyptoplasmic flourescene granules are identified respectively. Molecular characterization using polymerase chain reaction (PCR) using specific primer for G-Protein Coupled Chemokine Receptor Gene of LSDV isolates specific amplified product 554 bp. Sequence analysis revealed tow new isolates of LSDV. ARTICLE INFO
{"title":"Serological and molecular characterization of recent lumpy skin disease virus isolates from naturally infected previously vaccinated cattle in Egypt","authors":"Tamam S.M., E. N.M, Shokier K.A","doi":"10.21608/jvmr.2018.43304","DOIUrl":"https://doi.org/10.21608/jvmr.2018.43304","url":null,"abstract":"lumpy skin disease virus (LSDV) was isolated, from naturally infected cattle that have a history of previous vaccination with live attenuated sheep pox virus (SPV) vaccine. The virus was isolated on chorio-allantoic membranes (CAM) of embryonated chicken eggs (ECE) and Madin Darby Bovine Kidney Cells (MDBK) and identified by agar gel precipitation test (AGPT) and immunofluorescent antibody technique (IFA). Characteristic pock lesions and intracyptoplasmic flourescene granules are identified respectively. Molecular characterization using polymerase chain reaction (PCR) using specific primer for G-Protein Coupled Chemokine Receptor Gene of LSDV isolates specific amplified product 554 bp. Sequence analysis revealed tow new isolates of LSDV. ARTICLE INFO","PeriodicalId":53046,"journal":{"name":"Journal of Veterinary Medical Research","volume":"70 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83209898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-06-01DOI: 10.21608/jvmr.2018.43308
F. A. Khalalfalla, Fatma H. M. Ali, Omnia A.M.Ahmed.
ARTICLE INFO A total of (120) sample of ready to eat (RTE) meat;(20) each of cooked luncheon, frankfurter, hot dog, pasterma, shawrma and smoked luncheon were collected from different shops and grocery stores in Beni-Suef City. To be examined for their microbial load for aerobic plate count (APC), most probable number (MPN) of coliforms, fecal coliforms and E.coli as well as Staphylococcus aureus and enterococci were enumerated. Additionally E.coli, salmonella and Listeria were isolated and identified biochemically. Aerobic plate count (APC) had the highest mean value in shawerma (1×10 7 ± 5×10 6 CFU/g), shawerma also showed the highest most probable number (MPN) of coliforms and fecal coliforms (11 and 6 CFU/g) mean while pasterma was the highest contaminated with Staphylococcus aureus (3×10 4 CFU/g) and the highest count of enterococci was detected in hot dog (3×10 5 CFU/g). On the other hand each of E.coli, Salmonella and Listeria species were failed to be detected in any of the examined samples. The public health significance as well as the recommendations to produce safe and high quality ready to eat (RTE) meat products were mentioned. Article history:
{"title":"Microbiological risk assessment in ready to eat processed meat","authors":"F. A. Khalalfalla, Fatma H. M. Ali, Omnia A.M.Ahmed.","doi":"10.21608/jvmr.2018.43308","DOIUrl":"https://doi.org/10.21608/jvmr.2018.43308","url":null,"abstract":"ARTICLE INFO A total of (120) sample of ready to eat (RTE) meat;(20) each of cooked luncheon, frankfurter, hot dog, pasterma, shawrma and smoked luncheon were collected from different shops and grocery stores in Beni-Suef City. To be examined for their microbial load for aerobic plate count (APC), most probable number (MPN) of coliforms, fecal coliforms and E.coli as well as Staphylococcus aureus and enterococci were enumerated. Additionally E.coli, salmonella and Listeria were isolated and identified biochemically. Aerobic plate count (APC) had the highest mean value in shawerma (1×10 7 ± 5×10 6 CFU/g), shawerma also showed the highest most probable number (MPN) of coliforms and fecal coliforms (11 and 6 CFU/g) mean while pasterma was the highest contaminated with Staphylococcus aureus (3×10 4 CFU/g) and the highest count of enterococci was detected in hot dog (3×10 5 CFU/g). On the other hand each of E.coli, Salmonella and Listeria species were failed to be detected in any of the examined samples. The public health significance as well as the recommendations to produce safe and high quality ready to eat (RTE) meat products were mentioned. Article history:","PeriodicalId":53046,"journal":{"name":"Journal of Veterinary Medical Research","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75802709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-06-01DOI: 10.21608/JVMR.2018.43306
HebatAllah H. Mahmoud, M. Gad, A. Awaad, M. Tawfiek
ARTICLE INFO The aim of the present work was to describe the way of creating chosen interactive and animated three-dimensional anatomic virtual models of particular parts of birds' skeleton. These models can be used for teaching purposes in anatomy courses to assist veterinary students, and veterinarians to know the different structures of birds’ bones without dissection. We used available multimedia technologies to develop a simple computer program for visualization and annotation of threedimensional duck skeleton. The entire program involves three-dimensional realistic objects, description texts, and images, in addition to quizzes for users' selfassessment. Addressing the anatomical structures made the program more useful to promote knowledge, enhance and facilitate teaching and learning of birds’ osteology for veterinary students. *Corresponding author. Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt. E.mail: d.hebatallah@yahoo.com Article history:
{"title":"Development of an interactive three-dimensional virtual skeleton of birds for use in anatomical education.","authors":"HebatAllah H. Mahmoud, M. Gad, A. Awaad, M. Tawfiek","doi":"10.21608/JVMR.2018.43306","DOIUrl":"https://doi.org/10.21608/JVMR.2018.43306","url":null,"abstract":"ARTICLE INFO The aim of the present work was to describe the way of creating chosen interactive and animated three-dimensional anatomic virtual models of particular parts of birds' skeleton. These models can be used for teaching purposes in anatomy courses to assist veterinary students, and veterinarians to know the different structures of birds’ bones without dissection. We used available multimedia technologies to develop a simple computer program for visualization and annotation of threedimensional duck skeleton. The entire program involves three-dimensional realistic objects, description texts, and images, in addition to quizzes for users' selfassessment. Addressing the anatomical structures made the program more useful to promote knowledge, enhance and facilitate teaching and learning of birds’ osteology for veterinary students. *Corresponding author. Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt. E.mail: d.hebatallah@yahoo.com Article history:","PeriodicalId":53046,"journal":{"name":"Journal of Veterinary Medical Research","volume":"103 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73847447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-06-01DOI: 10.21608/jvmr.2018.43300
Walid H. Hassan, Ahmed H. Abed, Soad Abd-El Aziz Abd-Alwanis, Marwa Ahmed Yahia Al-Sayed
{"title":"Phenotypic and genotypic characterization of oxidase positive Gram negative bacilli isolated from broiler chickens.","authors":"Walid H. Hassan, Ahmed H. Abed, Soad Abd-El Aziz Abd-Alwanis, Marwa Ahmed Yahia Al-Sayed","doi":"10.21608/jvmr.2018.43300","DOIUrl":"https://doi.org/10.21608/jvmr.2018.43300","url":null,"abstract":"","PeriodicalId":53046,"journal":{"name":"Journal of Veterinary Medical Research","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87028543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-06-01DOI: 10.21608/JVMR.2018.43301
Amany T. El-Gendy, Abdel-Hamid I. Bazid, H. Madbouly, S. Tamam
Outbreaks of Foot and Mouth Disease (FMD) cause severe economic loss to the livestock industries in terms of loss of meat, milk production and the high mortality rateespecially in calves. This study was intended for detection, isolation and molecular characterization of FMDV circulated strain among different regions in Beni-Suef governorate, Egypt during 2015 and 2017. One hundred field samples were collected from clinically diseased cattle and buffaloes including vesicular fluid and sloughed epithelial membrane. Molecular detection and differentiation of FMDV serotypes by RT-PCR showed that (71%) were positive for serotype O indicating its predominance in Beni-Suef, Egypt.Also (18%) were positive for serotype SAT2 and (7%) were un-typed serotype being only positive for universal primer. Twenty samples were isolated on BHK-21 cells clone 13. Three samples showed the characteristic CPE of FMDV after blind passage 4 times. Sequences of VP1 coding-region of the three isolated FMD virus showed that the three isolated viruses were serotype O. The phylogenetic analysis of the isolated viruses reveled that they were closely related to type O of those reported in Ismailia, Behira, Giza and Cairo during 2016 with identity ranged from 97.7% - 99.8%. The most relevant outboard isolate was SUD/8/2008 with 93% identity after Blast homology search. However, the phylogenetic tree analysis revealed that the three FMDV type O isolates FMDV/serotype O/Beni-Suef/2017 differs partially from all other Egyptian serotypes of 2016. In conclusion, serotype O was the most prevalent FMDV serotype in Beni-Suef, Egypt.
{"title":"Molecular characterization and VP1 sequence analysis of Foot and Mouth disease new virus strains isolated during 2015-2017 in Beni-Suef, Egypt","authors":"Amany T. El-Gendy, Abdel-Hamid I. Bazid, H. Madbouly, S. Tamam","doi":"10.21608/JVMR.2018.43301","DOIUrl":"https://doi.org/10.21608/JVMR.2018.43301","url":null,"abstract":"Outbreaks of Foot and Mouth Disease (FMD) cause severe economic loss to the livestock industries in terms of loss of meat, milk production and the high mortality rateespecially in calves. This study was intended for detection, isolation and molecular characterization of FMDV circulated strain among different regions in Beni-Suef governorate, Egypt during 2015 and 2017. One hundred field samples were collected from clinically diseased cattle and buffaloes including vesicular fluid and sloughed epithelial membrane. Molecular detection and differentiation of FMDV serotypes by RT-PCR showed that (71%) were positive for serotype O indicating its predominance in Beni-Suef, Egypt.Also (18%) were positive for serotype SAT2 and (7%) were un-typed serotype being only positive for universal primer. Twenty samples were isolated on BHK-21 cells clone 13. Three samples showed the characteristic CPE of FMDV after blind passage 4 times. Sequences of VP1 coding-region of the three isolated FMD virus showed that the three isolated viruses were serotype O. The phylogenetic analysis of the isolated viruses reveled that they were closely related to type O of those reported in Ismailia, Behira, Giza and Cairo during 2016 with identity ranged from 97.7% - 99.8%. The most relevant outboard isolate was SUD/8/2008 with 93% identity after Blast homology search. However, the phylogenetic tree analysis revealed that the three FMDV type O isolates FMDV/serotype O/Beni-Suef/2017 differs partially from all other Egyptian serotypes of 2016. In conclusion, serotype O was the most prevalent FMDV serotype in Beni-Suef, Egypt.","PeriodicalId":53046,"journal":{"name":"Journal of Veterinary Medical Research","volume":"80 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85311087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-06-01DOI: 10.21608/JVMR.2018.43311
M. M. Hussein, W. Hassan, Aya M. A. El-Wkeel
ARTICLE INFO Fish with bacterial septicemic syndrome (BSS) exhibit very similar clinical signs regardless of the etiological agents. Members of Aeromonas, Pseudomonas, Vibrio, Edwardsiella, Streptococcus and Lactococcus species are considered the most reported bacterial pathogens incriminated in such syndrome. Aeromonas hydrophila, Edwardsiella tarda and Streptococcus iniae are 3 major pathogens share in the BSS associated losses in aquaculture and considered problematic for growth of tilapia and catfish production in Egypt. Therefore, rapid and accurate diagnosis is highly needed for controlling their disease outbreaks, particularly, in mixed infections. In an attempt to elucidate the main causative pathogen, a novel multiplex PCR (m-PCR) was newly designed in this study. The developed m-PCR involves amplifying the three multiple genes in single reaction based upon primers deduced from the regions carrying 16S rRNA, etfA and 16S RNA genes of A. hydrophila (Aeromonas spp.), E. tarda and S. iniae, respectively. Prior to perform m-PCR, individual PCR assays were carried out to adapt suitable laboratory and m-PCR assays conditions. The specificity of the developed m-PCR was confirmed by the fact that only specific fragments were amplified equivalent for 953, 415 and 300 bp corresponding to A.hydrophila, E. tarda and S. iniae, respectively, and that was evident with both extracted DNAs and the bacterial cells. More specifically, these specific bands were obtained also when either the extracted DNAs or the bacterial cells of the three pathogens mixed together in the reaction. The developed m-PCR is accurate, sensitive, fast and simple technique for the simultaneous detection of A. hydrophila (Aeromonas spp.), E.tarda, and S. iniae, three major bacterial pathogens involved in BSS incidence in Egypt. ARTICLE history:
{"title":"Multiplex PCR for simultaneous detection of 3 major fish pathogens incriminated in bacterial septicemic syndrome","authors":"M. M. Hussein, W. Hassan, Aya M. A. El-Wkeel","doi":"10.21608/JVMR.2018.43311","DOIUrl":"https://doi.org/10.21608/JVMR.2018.43311","url":null,"abstract":"ARTICLE INFO Fish with bacterial septicemic syndrome (BSS) exhibit very similar clinical signs regardless of the etiological agents. Members of Aeromonas, Pseudomonas, Vibrio, Edwardsiella, Streptococcus and Lactococcus species are considered the most reported bacterial pathogens incriminated in such syndrome. Aeromonas hydrophila, Edwardsiella tarda and Streptococcus iniae are 3 major pathogens share in the BSS associated losses in aquaculture and considered problematic for growth of tilapia and catfish production in Egypt. Therefore, rapid and accurate diagnosis is highly needed for controlling their disease outbreaks, particularly, in mixed infections. In an attempt to elucidate the main causative pathogen, a novel multiplex PCR (m-PCR) was newly designed in this study. The developed m-PCR involves amplifying the three multiple genes in single reaction based upon primers deduced from the regions carrying 16S rRNA, etfA and 16S RNA genes of A. hydrophila (Aeromonas spp.), E. tarda and S. iniae, respectively. Prior to perform m-PCR, individual PCR assays were carried out to adapt suitable laboratory and m-PCR assays conditions. The specificity of the developed m-PCR was confirmed by the fact that only specific fragments were amplified equivalent for 953, 415 and 300 bp corresponding to A.hydrophila, E. tarda and S. iniae, respectively, and that was evident with both extracted DNAs and the bacterial cells. More specifically, these specific bands were obtained also when either the extracted DNAs or the bacterial cells of the three pathogens mixed together in the reaction. The developed m-PCR is accurate, sensitive, fast and simple technique for the simultaneous detection of A. hydrophila (Aeromonas spp.), E.tarda, and S. iniae, three major bacterial pathogens involved in BSS incidence in Egypt. ARTICLE history:","PeriodicalId":53046,"journal":{"name":"Journal of Veterinary Medical Research","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89201811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-06-01DOI: 10.21608/jvmr.2018.43310
I. Radwan, Ahmed Osama El Gendey, M. F. Mohamed, N. Mohsen
{"title":"Multi-drug Resistant Enterococcus faecalis isolated from animal and human sources","authors":"I. Radwan, Ahmed Osama El Gendey, M. F. Mohamed, N. Mohsen","doi":"10.21608/jvmr.2018.43310","DOIUrl":"https://doi.org/10.21608/jvmr.2018.43310","url":null,"abstract":"","PeriodicalId":53046,"journal":{"name":"Journal of Veterinary Medical Research","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82024494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-06-01DOI: 10.21608/jvmr.2018.43309
W. Hassan, A. Abed, A. Thabet, E. A. E. Nady
{"title":"Genetic analysis of multidrug resistant Salmonella isolated from broiler chickens","authors":"W. Hassan, A. Abed, A. Thabet, E. A. E. Nady","doi":"10.21608/jvmr.2018.43309","DOIUrl":"https://doi.org/10.21608/jvmr.2018.43309","url":null,"abstract":"","PeriodicalId":53046,"journal":{"name":"Journal of Veterinary Medical Research","volume":"156 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79887546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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