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Enhanced editing efficiency in Arabidopsis with a LbCas12a variant harboring D156R and E795L mutations 利用携带 D156R 和 E795L 突变的 LbCas12a 变体提高拟南芥的编辑效率
IF 4.6 4区 农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-03-26 DOI: 10.1007/s42994-024-00144-w
Cuiping Xin, Dexin Qiao, Junya Wang, Wei Sun, Zhenghong Cao, Yu Lu, Yuanyuan Jiang, Yiping Chai, Xue-Chen Wang, Qi-jun Chen

Cas12a (Cpf1), a Class 2 Type V CRISPR/Cas nuclease, has several unique attributes for genome editing and may provide a valuable alternative to Cas9. However, a low editing efficiency due to temperature sensitivity and insufficient cleavage activity of the Cas12a nuclease are major obstacles to its broad application. In this report, we generated two variants, ttAsCas12 Ultra and ttLbCas12a Ultra harboring three (E174R, M537R, and F870L) or two (D156R and E795L) mutations, respectively, by combining the mutations from the temperature-tolerant variants ttAsCas12a (E174R) and ttLbCas12a (D156R), and those from the highly active variants AsCas12a Ultra (M537R and F870L) and LbCas12a Ultra (E795L). We compared editing efficiencies of the five resulting Cas12a variants (LbCas12a, ttLbCas12a, ttLbCas12a Ultra, AsCas12a Ultra, and ttAsCas12 Ultra) at six target sites of four genes in Arabidopsis (Arabidopsis thaliana). The variant ttLbCas12a Ultra, harboring the D156R and E795L mutations, exhibited the highest editing efficiency of all variants tested in Arabidopsis and can be used to generate homozygous or biallelic mutants in a single generation in Arabidopsis plants grown at 22 °C. In addition, optimization of ttLbCas12a Ultra, by varying nuclear localization signal sequences and codon usage, further greatly improved editing efficiency. Collectively, our results indicate that ttLbCas12a Ultra is a valuable alternative to Cas9 for editing genes or promoters in Arabidopsis.

Cas12a(Cpf1)是一种2类V型CRISPR/Cas核酸酶,在基因组编辑方面具有一些独特的特性,可作为Cas9的重要替代品。然而,Cas12a核酸酶对温度的敏感性导致编辑效率较低,而且其裂解活性不足,这些都是其广泛应用的主要障碍。在本报告中,我们生成了两个变体,ttAsCas12 Ultra 和 ttLbCas12a Ultra,它们分别携带三个(E174R、M537R 和 F870L)或两个(D156R 和 E795L)突变、将耐温变体ttAsCas12a(E174R)和ttLbCas12a(D156R)的突变与高活性变体AsCas12a Ultra(M537R和F870L)和LbCas12a Ultra(E795L)的突变结合起来。我们比较了所产生的五个 Cas12a 变体(LbCas12a、ttLbCas12a、ttLbCas12a Ultra、AsCas12a Ultra 和 ttAsCas12 Ultra)在拟南芥(Arabidopsis thaliana)四个基因的六个靶位点的编辑效率。变体ttLbCas12a Ultra携带D156R和E795L突变,在拟南芥中的所有测试变体中表现出最高的编辑效率,可用于在22 ℃下生长的拟南芥植株中单代产生同源或双倍突变体。此外,通过改变核定位信号序列和密码子的使用,ttLbCas12a Ultra 的优化进一步大大提高了编辑效率。总之,我们的研究结果表明,ttLbCas12a Ultra 是拟南芥中用于编辑基因或启动子的 Cas9 的重要替代品。
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引用次数: 0
Revealing the specific regulations of nitric oxide on the postharvest ripening and senescence of bitter melon fruit 揭示一氧化氮对苦瓜果实采后成熟和衰老的特殊调节作用
IF 4.6 4区 农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-03-21 DOI: 10.1007/s42994-023-00110-y
Hongwei Wang, Ling Li, Lili Ma, Alisdair R. Fernie, Anzhen Fu, Chunmei Bai, Zhaoze Sang, Susu Guo, Fan Zhang, Qing Wang, Yanyan Zheng, Jinhua Zuo

Bitter melon fruit is susceptible to yellowing, softening, and rotting under room-temperature storage conditions, resulting in reduced commercial value. Nitric oxide (NO) is an important signaling molecule and plays a crucial role in regulating the fruit postharvest quality. In this study, we investigated the effects of NO treatment on changes in sensory and firmness of bitter melon fruit during postharvest storage. Moreover, transcriptomic, metabolomic, and proteomic analyses were performed to elucidate the regulatory mechanisms through which NO treatment delays the ripening and senescence of bitter melon fruit. Our results show that differentially expressed genes (DEGs) were involved in fruit texture (CSLE, β-Gal, and PME), plant hormone signal transduction (ACS, JAR4, and AUX28), and fruit flavor and aroma (SUS2, LOX, and GDH2). In addition, proteins differentially abundant were associated with fruit texture (PLY, PME, and PGA) and plant hormone signal transduction (PBL15, JAR1, and PYL9). Moreover, NO significantly increased the abundance of key enzymes involved in the phenylpropanoid biosynthetic pathway, thus enhancing the disease resistance and alleviating softening of bitter melon fruit. Finally, differential metabolites mainly included phenolic acids, terpenoids, and flavonoids. These results provide a theoretical basis for further studies on the physiological changes associated with postharvest ripening and senescence of bitter melon fruit.

在室温贮藏条件下,苦瓜果实容易变黄、软化和腐烂,导致商业价值降低。一氧化氮(NO)是一种重要的信号分子,在调节果实采后品质方面起着至关重要的作用。本研究调查了一氧化氮处理对采后贮藏期间苦瓜果实感官和硬度变化的影响。此外,我们还进行了转录组、代谢组和蛋白质组分析,以阐明氮氧化物处理延缓苦瓜果实成熟和衰老的调控机制。结果表明,差异表达基因(DEGs)涉及果实质地(CSLE、β-Gal和PME)、植物激素信号转导(ACS、JAR4和AUX28)以及果实风味和香气(SUS2、LOX和GDH2)。此外,与果实质地(PLY、PME 和 PGA)和植物激素信号转导(PBL15、JAR1 和 PYL9)相关的蛋白质丰度存在差异。此外,氮氧化物明显增加了参与苯丙类生物合成途径的关键酶的丰度,从而提高了苦瓜的抗病性,减轻了苦瓜果实的软化。最后,差异代谢产物主要包括酚酸类、萜类和黄酮类化合物。这些结果为进一步研究与苦瓜果实采后成熟和衰老相关的生理变化提供了理论依据。
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引用次数: 0
Fusion of a rice endogenous N-methylpurine DNA glycosylase to a plant adenine base transition editor ABE8e enables A-to-K base editing in rice plants 将水稻内源 N-甲基嘌呤 DNA 糖基化酶与植物腺嘌呤碱基转换编辑器 ABE8e 融合,可在水稻植株中进行 A-K 碱基编辑
IF 4.6 4区 农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-03-21 DOI: 10.1007/s42994-024-00138-8
Yucai Li, Shaoya Li, Chenfei Li, Chen Zhang, Lei Yan, Jingying Li, Yubing He, Yan Guo, Lanqin Xia

Engineering of a new type of plant base editor for simultaneous adenine transition and transversion within the editing window will greatly expand the scope and potential of base editing in directed evolution and crop improvement. Here, we isolated a rice endogenous hypoxanthine excision protein, N-methylpurine DNA glycosylase (OsMPG), and engineered two plant A-to-K (K = G or T) base editors, rAKBE01 and rAKBE02, for simultaneous adenine transition and transversion base editing in rice by fusing OsMPG or its mutant mOsMPG to a plant adenine transition base editor, ABE8e. We further coupled either OsMPG or mOsMPG with a transactivation factor VP64 to generate rAKBE03 and rAKBE04, respectively. Testing these four rAKBEs, at five endogenous loci in rice protoplasts, indicated that rAKBE03 and rAKBE04 enabled higher levels of A-to-G base transitions when compared to ABE8e and ABE8e-VP64. Furthermore, whereas rAKBE01 only enabled A-to-C/T editing at one endogenous locus, in comparison with rAKBE02 and rAKBE03, rAKBE04 could significantly improve the A-to-C/T base transversion efficiencies by up to 6.57- and 1.75-fold in the rice protoplasts, respectively. Moreover, although no stable lines with A-to-C transversion were induced by rAKBE01 and rAKBE04, rAKBE04 could enable simultaneous A-to-G and A-to-T transition and transversion base editing, at all the five target loci, with the efficiencies of A-to-G transition and A-to-T transversion editing ranging from 70.97 to 92.31% and 1.67 to 4.84% in rice stable lines, respectively. Together, these rAKBEs enable different portfolios of editing products and, thus, now expands the potential of base editing in diverse application scenario for crop improvement.

在编辑窗口内同时进行腺嘌呤转换和反转的新型植物碱基编辑工程将极大地扩展碱基编辑在定向进化和作物改良中的应用范围和潜力。在这里,我们分离了一种水稻内源次黄嘌呤切除蛋白--N-甲基嘌呤DNA糖基化酶(OsMPG),并通过将OsMPG或其突变体mOsMPG与植物腺嘌呤转换碱基编辑器ABE8e融合,设计了两种植物A-to-K(K = G或T)碱基编辑器--rAKBE01和rAKBE02,用于在水稻中同时进行腺嘌呤转换和反转碱基编辑。我们进一步将 OsMPG 或 mOsMPG 与转录激活因子 VP64 结合,分别产生了 rAKBE03 和 rAKBE04。在水稻原生质体的五个内源位点上测试这四个 rAKBE,结果表明与 ABE8e 和 ABE8e-VP64 相比,rAKBE03 和 rAKBE04 能够实现更高水平的 A-G 碱基转换。此外,与 rAKBE02 和 rAKBE03 相比,rAKBE01 只能在一个内源基因座上实现 A-C/T 编辑,而 rAKBE04 则能显著提高水稻原生质体中 A-C/T 碱基转换效率,分别高达 6.57 倍和 1.75 倍。此外,虽然 rAKBE01 和 rAKBE04 没有诱导出具有 A-C 转换的稳定品系,但 rAKBE04 可在所有五个目标基因座上同时实现 A-G 和 A-T 转换和反转碱基编辑,在水稻稳定品系中的 A-G 转换和 A-T 反转编辑效率分别为 70.97% 至 92.31% 和 1.67% 至 4.84%。这些 rAKBEs 可提供不同的编辑产品组合,从而拓展了碱基编辑在作物改良中的多种应用前景。
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引用次数: 0
A sensitive one-pot ROA assay for rapid miRNA detection 用于快速检测 miRNA 的灵敏的一次性 ROA 分析法
IF 4.6 4区 农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-03-18 DOI: 10.1007/s42994-024-00140-0
Zhihao Hou, Wenpeng Deng, Alun Li, Ya Zhang, Jianye Chang, Xinyue Guan, Yuxiao Chang, Kaile Wang, Xinjie Wang, Jue Ruan

MicroRNAs (miRNAs) and short RNA fragments (18–25 nt) are crucial biomarkers in biological research and disease diagnostics. However, their accurate and rapid detection remains a challenge, largely due to their low abundance, short length, and sequence similarities. In this study, we report on a highly sensitive, one-step RNA O-circle amplification (ROA) assay for rapid and accurate miRNA detection. The ROA assay commences with the hybridization of a circular probe with the test RNA, followed by a linear rolling circle amplification (RCA) using dUTP. This amplification process is facilitated by U-nick reactions, which lead to an exponential amplification for readout. Under optimized conditions, assays can be completed within an hour, producing an amplification yield up to the microgram level, with a detection limit as low as 0.15 fmol (6 pM). Notably, the ROA assay requires only one step, and the results can be easily read visually, making it user-friendly. This ROA assay has proven effective in detecting various miRNAs and phage ssRNA. Overall, the ROA assay offers a user-friendly, rapid, and accurate solution for miRNA detection.

微RNA(miRNA)和短RNA片段(18-25 nt)是生物研究和疾病诊断中的重要生物标志物。然而,主要由于其丰度低、长度短和序列相似性,准确快速地检测它们仍然是一项挑战。在这项研究中,我们报告了一种高灵敏度、一步式 RNA O-circle 扩增(ROA)测定法,用于快速准确地检测 miRNA。ROA 检测法首先将圆形探针与检测 RNA 杂交,然后使用 dUTP 进行线性滚圆扩增(RCA)。U-nick 反应促进了这一扩增过程,从而导致指数扩增读数。在优化条件下,检测可在一小时内完成,扩增产率可达微克级,检测限低至 0.15 fmol(6 pM)。值得注意的是,ROA 检测只需一个步骤,而且结果可以很容易地直观读取,方便用户使用。这种 ROA 检测法已被证明能有效检测各种 miRNA 和噬菌体 ssRNA。总之,ROA 检测法为 miRNA 检测提供了一种用户友好、快速而准确的解决方案。
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引用次数: 0
The RUBY reporter for visual selection in soybean genome editing 用于大豆基因组编辑视觉选择的 RUBY 报告器
IF 4.6 4区 农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-03-18 DOI: 10.1007/s42994-024-00148-6
Li Chen, Yupeng Cai, Xiaoqian Liu, Weiwei Yao, Shuiqing Wu, Wensheng Hou

Current systems to screen for transgenic soybeans (Glycine max) involve laborious molecular assays or the expression of fluorescent markers that are difficult to see in soybean plants. Therefore, a visual system for early screening of transgenic plants would increase the efficiency of crop improvement by genome editing. The RUBY reporter system, which consists of three genes encoding betalain biosynthetic enzymes, leading to the accumulation of purple pigment in transgenic tissue, has been employed in some plants and dikaryon fungi. Here, we assessed the RUBY reporter for visual verification during soybean transformation. We show that RUBY can be expressed in soybean, allowing for visual confirmation of transgenic events without the need for specialized equipment. Plants with visible accumulation of purple pigment in any tissue were successfully transformed, confirming the accuracy of the RUBY system as a visual indicator. We also assessed the genetic stability of the transgene across generations, which can be performed very early, using the cotyledons of the progeny. Transgene-free seedlings have a distinct green color, facilitating the selection of genome-edited but transgene-free soybean seedlings for harvest. Using the RUBY system, we quickly identified a transgene-free Gmwaxy mutant in the T1 generation. This system thus provides an efficient and convenient tool for soybean genome editing.

目前筛选转基因大豆(Glycine max)的系统涉及费力的分子检测或荧光标记的表达,而荧光标记在大豆植株中很难看到。因此,用于早期筛选转基因植物的可视系统将提高通过基因组编辑改良作物的效率。RUBY 报告系统由三个编码甜菜红素生物合成酶的基因组成,可导致转基因组织中紫色色素的积累,已在一些植物和二核真菌中使用。在此,我们对 RUBY 报告物进行了评估,以便在大豆转化过程中进行视觉验证。我们的研究表明,RUBY 可以在大豆中表达,从而无需专门设备即可对转基因事件进行视觉确认。在任何组织中都能看到紫色色素积累的植株被成功转化,这证实了 RUBY 系统作为视觉指标的准确性。我们还利用后代的子叶评估了转基因在不同世代间的遗传稳定性。不含转基因的幼苗具有明显的绿色,便于选择经过基因组编辑但不含转基因的大豆幼苗进行收获。利用 RUBY 系统,我们很快就在 T1 代中鉴定出了无转基因的 Gmwaxy 突变体。因此,该系统为大豆基因组编辑提供了一种高效便捷的工具。
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引用次数: 0
Impact of acute heat stress on mitochondrial function, ultrastructure and cardiolipin distribution in Arabidopsis 急性热胁迫对拟南芥线粒体功能、超微结构和心磷脂分布的影响
IF 4.6 4区 农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-03-15 DOI: 10.1007/s42994-024-00151-x
Yukang Wang, Ronghui Pan, Jianping Hu

Besides providing energy to sustain life, mitochondria also play crucial roles in stress response and programmed cell death. The mitochondrial hallmark lipid, cardiolipin (CL), is essential to the maintenance of mitochondrial structure and function. However, how mitochondria and CL are involved in stress response is not as well defined in plants as in animal and yeast cells. We previously revealed a role for CL in mitochondrial fission and in heat stress response in Arabidopsis. To further determine the involvement of mitochondria and CL in plant heat response, here we treated Arabidopsis seedlings with varied lengths of acute heat stress. These treatments resulted in decreases in mitochondrial membrane potential, disruption of mitochondrial ultrastructure, accumulation of mitochondrial reactive-oxygen species (ROS), and redistribution of CL to the outer mitochondrial membrane and to a novel type of vesicle. The level of the observed changes correlated with the severeness of the heat stress, indicating the strong relevance of these processes to stress response. Our findings provide the basis for studying mechanisms underpinning the role of mitochondria and CL in plant stress response.

线粒体除了为维持生命提供能量外,还在应激反应和细胞程序性死亡中发挥着至关重要的作用。线粒体的标志性脂质--心磷脂(CL)对线粒体结构和功能的维持至关重要。然而,线粒体和 CL 如何参与应激反应在植物中还没有像在动物和酵母细胞中那样明确。我们之前揭示了 CL 在拟南芥线粒体裂变和热胁迫响应中的作用。为了进一步确定线粒体和 CL 在植物热响应中的作用,我们对拟南芥幼苗进行了不同时间长度的急性热胁迫处理。这些处理导致线粒体膜电位下降、线粒体超微结构破坏、线粒体活性氧(ROS)积累、CL重新分布到线粒体外膜和一种新型囊泡。观察到的变化程度与热应激的严重程度相关,表明这些过程与应激反应密切相关。我们的发现为研究线粒体和 CL 在植物胁迫响应中的作用机制奠定了基础。
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引用次数: 0
The maize ZmCPK39-ZmKnox2 module regulates plant height 玉米 ZmCPK39-ZmKnox2 模块调控植株高度
IF 4.6 4区 农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-03-15 DOI: 10.1007/s42994-024-00150-y
Mang Zhu, Chenyu Guo, Xiaohui Zhang, Yulin Liu, Xiaohui Jiang, Limei Chen, Mingliang Xu

Plant height is an important agronomic trait that affects high-density tolerance and lodging resistance. However, the regulators and their underlying molecular mechanisms controlling plant height in maize remain understudied. Here, we report that knockout mutants of the calcium-dependent protein kinase gene ZmCPK39 (ZmCPK39-KO) exhibit dramatically reduced plant height, characterized by shorter internodes and a slight decrease in node numbers. Furthermore, we identified a ZmCPK39-interacting protein, the knotted-related homeobox (ZmKnox2), and observed that plant height was also significantly reduced in a mutator transposon-inserted mutant of ZmKnox2 (ZmKnox2-Mu). Combined analysis of transcriptomic and metabonomic data indicates that multiple phytohormone signaling and photosynthesis pathways are disrupted in both ZmCPK39-KO and ZmKnox2-Mu mutants. Taken together, these results provide new insights into the function of ZmCPK39 and identify potential targets for breeding lodging-resistant and high-density tolerant maize cultivars.

株高是影响高密度耐受性和抗倒伏性的重要农艺性状。然而,控制玉米株高的调节因子及其潜在的分子机制仍未得到充分研究。在此,我们报告了钙依赖性蛋白激酶基因 ZmCPK39(ZmCPK39-KO)的基因敲除突变体表现出显著的植株高度降低,其特征是节间变短和节数略有减少。此外,我们还发现了一种与 ZmCPK39 相互作用的蛋白--结相关同源染色体(ZmKnox2),并观察到 ZmKnox2 的突变体转座子插入突变体(ZmKnox2-Mu)的株高也显著降低。对转录组和代谢组数据的综合分析表明,ZmCPK39-KO和ZmKnox2-Mu突变体的多种植物激素信号转导和光合作用途径都受到了干扰。综上所述,这些结果为了解 ZmCPK39 的功能提供了新的视角,并为培育抗倒伏和高密度耐受性玉米栽培品种确定了潜在的目标。
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引用次数: 0
Integrating machine learning and genome editing for crop improvement 将机器学习与基因组编辑相结合,促进作物改良
IF 4.6 4区 农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-02-29 DOI: 10.1007/s42994-023-00133-5
Long Chen, Guanqing Liu, Tao Zhang

Genome editing is a promising technique that has been broadly utilized for basic gene function studies and trait improvements. Simultaneously, the exponential growth of computational power and big data now promote the application of machine learning for biological research. In this regard, machine learning shows great potential in the refinement of genome editing systems and crop improvement. Here, we review the advances of machine learning to genome editing optimization, with emphasis placed on editing efficiency and specificity enhancement. Additionally, we demonstrate how machine learning bridges genome editing and crop breeding, by accurate key site detection and guide RNA design. Finally, we discuss the current challenges and prospects of these two techniques in crop improvement. By integrating advanced genome editing techniques with machine learning, progress in crop breeding will be further accelerated in the future.

基因组编辑是一项前景广阔的技术,已被广泛用于基础基因功能研究和性状改良。与此同时,计算能力和大数据的指数级增长促进了机器学习在生物学研究中的应用。在这方面,机器学习在完善基因组编辑系统和作物改良方面显示出巨大潜力。在此,我们回顾了机器学习在基因组编辑优化方面的进展,重点是编辑效率和特异性的提高。此外,我们还展示了机器学习如何通过精确的关键位点检测和导向 RNA 设计,在基因组编辑和作物育种之间架起桥梁。最后,我们讨论了这两种技术在作物改良中目前面临的挑战和前景。通过将先进的基因组编辑技术与机器学习相结合,未来将进一步加快作物育种的进展。
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引用次数: 0
Innovative computational tools provide new insights into the polyploid wheat genome 创新计算工具为多倍体小麦基因组提供了新见解
IF 4.6 4区 农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-02-07 DOI: 10.1007/s42994-023-00131-7
Yongming Chen, Wenxi Wang, Zhengzhao Yang, Huiru Peng, Zhongfu Ni, Qixin Sun, Weilong Guo

Bread wheat (Triticum aestivum) is an important crop and serves as a significant source of protein and calories for humans, worldwide. Nevertheless, its large and allopolyploid genome poses constraints on genetic improvement. The complex reticulate evolutionary history and the intricacy of genomic resources make the deciphering of the functional genome considerably more challenging. Recently, we have developed a comprehensive list of versatile computational tools with the integration of statistical models for dissecting the polyploid wheat genome. Here, we summarize the methodological innovations and applications of these tools and databases. A series of step-by-step examples illustrates how these tools can be utilized for dissecting wheat germplasm resources and unveiling functional genes associated with important agronomic traits. Furthermore, we outline future perspectives on new advanced tools and databases, taking into consideration the unique features of bread wheat, to accelerate genomic-assisted wheat breeding.

面包小麦(Triticum aestivum)是一种重要的农作物,是全球人类蛋白质和热量的重要来源。然而,其庞大的全多倍体基因组对遗传改良造成了限制。复杂的网状进化历史和错综复杂的基因组资源使得破译功能基因组的难度大大增加。最近,我们开发了一系列多功能计算工具,并整合了统计模型,用于剖析多倍体小麦基因组。在此,我们总结了这些工具和数据库在方法上的创新和应用。一系列循序渐进的例子说明了如何利用这些工具剖析小麦种质资源并揭示与重要农艺性状相关的功能基因。此外,考虑到面包小麦的独特性,我们概述了新的先进工具和数据库的未来前景,以加速基因组辅助小麦育种。
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引用次数: 0
DGS1 improves rice disease resistance by elevating pathogen-associated molecular pattern-triggered immunity DGS1 通过提高病原体相关分子模式触发的免疫力来提高水稻的抗病性
IF 4.6 4区 农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-02-06 DOI: 10.1007/s42994-024-00137-9
Yu Wang, Chuan Zheng, You-liang Peng, Qian Chen

Rice yield and disease resistance are two crucial factors in determining the suitability of a gene for agricultural breeding. Decreased grain size1 (DGS1), encoding an RING-type E3 ligase, has been found to have a positive effect on rice yield by regulating rice grain number and 1000-grain weight. However, the role of DGS1 in rice blast resistance is still unknown. In this study, we report that DGS1 enhances disease resistance by improving PTI responses, including stronger ROS burst and MAPK activation, and also increased expression of defense-related genes. Furthermore, DGS1 works in conjunction with ubiquitin conjugating enzyme OsUBC45 as an E2–E3 pair to facilitate the ubiquitin-dependent degradation of OsGSK3 and OsPIP2;1, thereby influencing rice yield and immunity, respectively. Therefore, the DGS1-OsUBC45 module has the potential in facilitating rice agricultural breeding.

水稻产量和抗病性是决定一个基因是否适合农业育种的两个关键因素。研究发现,编码 RING 型 E3 连接酶的减粒 1(DGS1)通过调节水稻粒数和千粒重对水稻产量有积极影响。然而,DGS1 在水稻稻瘟病抗性中的作用尚不清楚。在本研究中,我们报告了 DGS1 通过改善 PTI 反应(包括更强的 ROS 暴发和 MAPK 激活)以及增加防御相关基因的表达来增强抗病性。此外,DGS1 与泛素连接酶 OsUBC45 作为一对 E2-E3 共同作用,促进 OsGSK3 和 OsPIP2;1 的泛素依赖性降解,从而分别影响水稻产量和免疫力。因此,DGS1-OsUBC45 模块具有促进水稻农业育种的潜力。
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引用次数: 0
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