Pub Date : 2025-04-01eCollection Date: 2025-06-23DOI: 10.1021/cbmi.5c00012
Xinlan Zhou, Xinzhong Ruan, Xingjiang Li, Weiyuan Xu, Xinhui Xiao, Aiyi Chen, Yinghui Ding, Jilai Zhang, Gengshen Mo, Yong Jian, Xinyang Wu, Fangfu Ye, Zhiqiang Wang, Yi Li, Lixiong Dai
Magnetic resonance imaging (MRI) is a critical tool in medical diagnostics, yet conventional MRI contrast agents (CAs) are often limited by their small-molecule nature, resulting in rapid clearance and low relaxivity. This study presents a chiral strategy for developing high-performance polymeric gadolinium-based CAs, PAA-EOB-GdA and PAA-EOB-GdB, tailored for enhanced vascular and tumor imaging. Notably, PAA-EOB-GdA, a chiral Gd-DOTA derivative integrated with sodium poly-(acrylic acid) (PAA), benefits from the optimized water exchange rate of chiral Gd-(III) complex and the polymer effect of PAA, exhibiting exceptionally high relaxivity (r1 = 37.87 mM-1 s-1, 11.9-fold of clinical Gd-DOTA) and showed remarkable imaging efficacy in magnetic resonance angiography (MRA) with low-dose administration (0.05 mmol kg-1) and an extended imaging duration. Its performance in tumor imaging was also impressive, maintaining superior enhancement values compared to Gd-DOTA. These characteristics feature PAA-EOB-GdA as a promising candidate for clinical diagnosis in both vascular and tumor imaging applications.
{"title":"Chiral Strategy for Developing High-Performance Polymeric Gadolinium-Based MRI Contrast Agents for Vascular and Tumor Imaging.","authors":"Xinlan Zhou, Xinzhong Ruan, Xingjiang Li, Weiyuan Xu, Xinhui Xiao, Aiyi Chen, Yinghui Ding, Jilai Zhang, Gengshen Mo, Yong Jian, Xinyang Wu, Fangfu Ye, Zhiqiang Wang, Yi Li, Lixiong Dai","doi":"10.1021/cbmi.5c00012","DOIUrl":"10.1021/cbmi.5c00012","url":null,"abstract":"<p><p>Magnetic resonance imaging (MRI) is a critical tool in medical diagnostics, yet conventional MRI contrast agents (CAs) are often limited by their small-molecule nature, resulting in rapid clearance and low relaxivity. This study presents a chiral strategy for developing high-performance polymeric gadolinium-based CAs, PAA-EOB-GdA and PAA-EOB-GdB, tailored for enhanced vascular and tumor imaging. Notably, PAA-EOB-GdA, a chiral Gd-DOTA derivative integrated with sodium poly-(acrylic acid) (PAA), benefits from the optimized water exchange rate of chiral Gd-(III) complex and the polymer effect of PAA, exhibiting exceptionally high relaxivity (<i>r</i> <sub>1</sub> = 37.87 mM<sup>-1</sup> s<sup>-1</sup>, 11.9-fold of clinical Gd-DOTA) and showed remarkable imaging efficacy in magnetic resonance angiography (MRA) with low-dose administration (0.05 mmol kg<sup>-1</sup>) and an extended imaging duration. Its performance in tumor imaging was also impressive, maintaining superior enhancement values compared to Gd-DOTA. These characteristics feature PAA-EOB-GdA as a promising candidate for clinical diagnosis in both vascular and tumor imaging applications.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"3 6","pages":"387-397"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12188411/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144509403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aggregation-induced emission (AIE) materials, due to their unique ability to significantly enhance optical emission in aggregated states, have demonstrated vast potential in immunology, particularly in early disease diagnosis and immunotherapy. AIE materials can serve as highly efficient fluorescent probes for biomarker detection, providing critical insights for early diagnosis. Additionally, they can act as cancer vaccines, enhancing the effectiveness of immunotherapy through photodynamic therapy, photothermal therapy, or activation of immune cells. This review highlights the applications of AIE materials in immunology, focusing on recent advancements in disease immune diagnostics and immunotherapy while also discussing the challenges they face and potential directions for future development.
{"title":"Applications of Aggregation-Induced Emission Materials in Immunology: From Diagnostics to Immunotherapy","authors":"Langyi Yang, Ling-Hong Xiong and Xuewen He*, ","doi":"10.1021/cbmi.5c00016","DOIUrl":"https://doi.org/10.1021/cbmi.5c00016","url":null,"abstract":"<p >Aggregation-induced emission (AIE) materials, due to their unique ability to significantly enhance optical emission in aggregated states, have demonstrated vast potential in immunology, particularly in early disease diagnosis and immunotherapy. AIE materials can serve as highly efficient fluorescent probes for biomarker detection, providing critical insights for early diagnosis. Additionally, they can act as cancer vaccines, enhancing the effectiveness of immunotherapy through photodynamic therapy, photothermal therapy, or activation of immune cells. This review highlights the applications of AIE materials in immunology, focusing on recent advancements in disease immune diagnostics and immunotherapy while also discussing the challenges they face and potential directions for future development.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"3 8","pages":"499–521"},"PeriodicalIF":5.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/cbmi.5c00016","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144892574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-01eCollection Date: 2025-09-22DOI: 10.1021/cbmi.5c00007
Lei Zhang, Dongwen Shen, Jiazhen Yang
Recent advancements in single-molecule biophysics have been driven by breakthroughs in advanced fluorescence microscopy techniques and the development of next-generation organic fluorophores. These cutting-edge fluorophores, coupled through tailored biolabeling strategies, offer single-molecule brightness, photostability, and phototunability (i.e., photoswitchable, photoactivatable), contributing to enhancing spatial and temporal imaging resolution for studying biomolecular interactions and dynamics at single-event precision. This review examines the progress made over the past decade in the development of next-generation fluorophores, along with their site-specific labeling methods for proteins, nucleic acids, and biomolecular complexes. It also explores their applications in single-molecule fluorescence-based dynamic structural biology and super-resolution microscopy imaging. Furthermore, it examines ongoing efforts to address challenges associated with fluorophore photostability, photobleaching, and the integration of advanced photophysical and photochemical functionalities. The integration of state-of-the-art fluorophores with advanced labeling strategies aim to deliver complementary correlative data, holding promise for revolutionizing single-molecule biophysics by pushing the boundaries of temporal and spatial imaging resolution to unprecedented limits.
{"title":"Advancing Single-Molecule Biophysics: Next-Generation Organic Fluorophores with Tailored Labeling Strategies.","authors":"Lei Zhang, Dongwen Shen, Jiazhen Yang","doi":"10.1021/cbmi.5c00007","DOIUrl":"10.1021/cbmi.5c00007","url":null,"abstract":"<p><p>Recent advancements in single-molecule biophysics have been driven by breakthroughs in advanced fluorescence microscopy techniques and the development of next-generation organic fluorophores. These cutting-edge fluorophores, coupled through tailored biolabeling strategies, offer single-molecule brightness, photostability, and phototunability (i.e., photoswitchable, photoactivatable), contributing to enhancing spatial and temporal imaging resolution for studying biomolecular interactions and dynamics at single-event precision. This review examines the progress made over the past decade in the development of next-generation fluorophores, along with their site-specific labeling methods for proteins, nucleic acids, and biomolecular complexes. It also explores their applications in single-molecule fluorescence-based dynamic structural biology and super-resolution microscopy imaging. Furthermore, it examines ongoing efforts to address challenges associated with fluorophore photostability, photobleaching, and the integration of advanced photophysical and photochemical functionalities. The integration of state-of-the-art fluorophores with advanced labeling strategies aim to deliver complementary correlative data, holding promise for revolutionizing single-molecule biophysics by pushing the boundaries of temporal and spatial imaging resolution to unprecedented limits.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"3 9","pages":"572-598"},"PeriodicalIF":5.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12458005/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145151656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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