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Suppressing ROS Production of AIE Nanoprobes by Simple Matrices Optimization for CNS Cell Observation and Minimized Influence of Cytoskeleton Morphology. 通过简单基质优化抑制AIE纳米探针对中枢神经系统细胞的观察及对细胞骨架形态影响最小化。
Pub Date : 2024-10-15 eCollection Date: 2024-11-25 DOI: 10.1021/cbmi.4c00061
Xiaotong Chen, Yajing Jiang, Jiaxin Liu, Yu Tian, Yifan Deng, Xiaoqiong Li, Wenbo Wu, Ruoyu Zhang, Yulin Deng

The visualization of the central nervous system (CNS) has proposed stringent criteria for fluorescent probes, as the inevitable production of reactive oxygen species (ROS) or heat generated from most photoluminescent probes upon excitation can disturb the normal status of relatively delicate CNS cells. In this work, a red-emitting fluorogen with aggregation-induced emission (AIE) characteristics, known as DTF, was chosen as the model fluorogen to investigate whether the side effects of ROS and heat could be suppressed through easy-to-operate processes. Specifically, DTF was encapsulated with different amphiphilic matrices to yield AIE nanoprobes, and their photoluminescent properties, ROS production, and photothermal conversion rates were examined. BSA@DTF NPs possessed 1.3-fold brightness compared to that of DSPE-PEG@DTF NPs and F127@DTF NPs but its ROS generation efficiency is markedly decreased to only 2.4% of that produced by F127@DTF NPs. Meanwhile, BSA@DTF NPs showed a negligible photothermal effect. These features make BSA@DTF NPs favorable for long-term live cell imaging, particularly for fluorescent imaging of CNS cells. BSA@DTF NPs were able to sustain the normal state of HT-22 neuronal cells with continuous illumination for at least 25 min, and they also preserved the cytoskeleton of microglia BV-2 cells as the untreated control group. This work represents a successful but easy-to-operate process to suppress the ROS generation of red-emissive AIEgen, and it highlights the importance of minimizing the ROS generation of the fluorescent probes, particularly in the application of long-term imaging of CNS cells.

中枢神经系统(CNS)的可视化对荧光探针提出了严格的标准,因为大多数光致发光探针在激发时不可避免地产生活性氧(ROS)或产生的热量会干扰相对脆弱的CNS细胞的正常状态。在这项工作中,我们选择了一种具有聚集诱导发射(AIE)特征的红色荧光氟化物(DTF)作为模型氟化物,研究是否可以通过易于操作的工艺抑制ROS和热的副作用。具体来说,用不同的两亲性基质封装DTF以制备AIE纳米探针,并检测其光致发光性能、ROS生成和光热转化率。BSA@DTF NPs的亮度是DSPE-PEG@DTF NPs和F127@DTF NPs的1.3倍,但ROS生成效率明显下降,仅为F127@DTF NPs的2.4%。同时,BSA@DTF NPs的光热效应可以忽略不计。这些特点使得BSA@DTF NPs有利于长期活细胞成像,特别是中枢神经系统细胞的荧光成像。BSA@DTF NPs能够在连续光照下维持HT-22神经元细胞的正常状态至少25分钟,并且与未处理的对照组一样,它们也保留了小胶质细胞BV-2细胞的细胞骨架。这项工作代表了一个成功且易于操作的过程来抑制红发射AIEgen的ROS生成,并强调了减少荧光探针ROS生成的重要性,特别是在CNS细胞长期成像的应用中。
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引用次数: 0
Suppressing ROS Production of AIE Nanoprobes by Simple Matrices Optimization for CNS Cell Observation and Minimized Influence of Cytoskeleton Morphology 通过优化简单基质抑制 AIE 纳米探针产生 ROS,以观察中枢神经系统细胞并最大限度地减少细胞骨架形态的影响
Pub Date : 2024-10-15 DOI: 10.1021/cbmi.4c0006110.1021/cbmi.4c00061
Xiaotong Chen, Yajing Jiang, Jiaxin Liu, Yu Tian, Yifan Deng, Xiaoqiong Li*, Wenbo Wu*, Ruoyu Zhang* and Yulin Deng*, 

The visualization of the central nervous system (CNS) has proposed stringent criteria for fluorescent probes, as the inevitable production of reactive oxygen species (ROS) or heat generated from most photoluminescent probes upon excitation can disturb the normal status of relatively delicate CNS cells. In this work, a red-emitting fluorogen with aggregation-induced emission (AIE) characteristics, known as DTF, was chosen as the model fluorogen to investigate whether the side effects of ROS and heat could be suppressed through easy-to-operate processes. Specifically, DTF was encapsulated with different amphiphilic matrices to yield AIE nanoprobes, and their photoluminescent properties, ROS production, and photothermal conversion rates were examined. BSA@DTF NPs possessed 1.3-fold brightness compared to that of DSPE-PEG@DTF NPs and F127@DTF NPs but its ROS generation efficiency is markedly decreased to only 2.4% of that produced by F127@DTF NPs. Meanwhile, BSA@DTF NPs showed a negligible photothermal effect. These features make BSA@DTF NPs favorable for long-term live cell imaging, particularly for fluorescent imaging of CNS cells. BSA@DTF NPs were able to sustain the normal state of HT-22 neuronal cells with continuous illumination for at least 25 min, and they also preserved the cytoskeleton of microglia BV-2 cells as the untreated control group. This work represents a successful but easy-to-operate process to suppress the ROS generation of red-emissive AIEgen, and it highlights the importance of minimizing the ROS generation of the fluorescent probes, particularly in the application of long-term imaging of CNS cells.

中枢神经系统(CNS)的可视化对荧光探针提出了严格的标准,因为大多数光致发光探针在激发时不可避免地会产生活性氧(ROS)或热量,从而干扰相对脆弱的中枢神经系统细胞的正常状态。在这项工作中,我们选择了一种具有聚集诱导发射(AIE)特性的红色发光荧光原(即 DTF)作为模型荧光原,以研究是否可以通过易于操作的过程来抑制 ROS 和热量的副作用。具体而言,用不同的两亲基质包覆 DTF 以产生 AIE 纳米探针,并考察了它们的光致发光特性、ROS 生成和光热转换率。与DSPE-PEG@DTF NPs和F127@DTF NPs相比,BSA@DTF NPs具有1.3倍的亮度,但其ROS生成效率明显降低,仅为F127@DTF NPs的2.4%。同时,BSA@DTF NPs 的光热效应可以忽略不计。这些特点使得BSA@DTF NPs有利于长期活细胞成像,特别是中枢神经系统细胞的荧光成像。BSA@DTF NPs 能够在持续光照下维持 HT-22 神经元细胞的正常状态至少 25 分钟,而且与未经处理的对照组一样,它们还能保持小胶质细胞 BV-2 的细胞骨架。这项工作代表了一种成功但易于操作的抑制红色致敏 AIEgen 产生 ROS 的方法,它强调了尽量减少荧光探针产生 ROS 的重要性,尤其是在中枢神经系统细胞的长期成像应用中。
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引用次数: 0
Advances in Super-resolution Stimulated Raman Scattering Microscopy. 超分辨率受激拉曼散射显微镜研究进展。
Pub Date : 2024-10-04 eCollection Date: 2024-11-25 DOI: 10.1021/cbmi.4c00057
William J Tipping, Karen Faulds, Duncan Graham

Super-resolution optical imaging overcomes the diffraction limit in light microscopy to enable the visualization of previously invisible molecular details within a sample. The realization of super-resolution imaging based on stimulated Raman scattering (SRS) microscopy represents a recent area of fruitful development that has been used to visualize cellular structures in three dimensions, with multiple spectroscopic colors at the nanometer scale. Several fundamental approaches to achieving super-resolution SRS imaging have been reported, including optical engineering strategies, expansion microscopy, deconvolution image analysis, and photoswitchable SRS reporters as methods to break the diffraction limit. These approaches have enabled the visualization of biological structures, cellular interactions, and dynamics with unprecedented detail. In this Perspective, an overview of the current strategies and capabilities for achieving super-resolution SRS imaging will be highlighted together with an outlook on potential directions of this rapidly evolving field.

超分辨率光学成像克服了光学显微镜的衍射极限,使以前不可见的分子细节在样品中可视化。基于受激拉曼散射(SRS)显微镜的超分辨率成像的实现代表了最近一个富有成效的发展领域,该领域已被用于在三维空间中可视化细胞结构,在纳米尺度上具有多种光谱颜色。已经报道了实现超分辨率SRS成像的几种基本方法,包括光学工程策略,扩展显微镜,反卷积图像分析和光切换SRS报告作为突破衍射极限的方法。这些方法使生物结构、细胞相互作用和动力学的可视化具有前所未有的细节。在这一观点中,将概述当前实现超分辨率SRS成像的策略和能力,并展望这一快速发展领域的潜在方向。
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引用次数: 0
Advances in Super-resolution Stimulated Raman Scattering Microscopy 超分辨率受激拉曼散射显微镜的研究进展
Pub Date : 2024-10-04 DOI: 10.1021/cbmi.4c0005710.1021/cbmi.4c00057
William J. Tipping*, Karen Faulds and Duncan Graham*, 

Super-resolution optical imaging overcomes the diffraction limit in light microscopy to enable the visualization of previously invisible molecular details within a sample. The realization of super-resolution imaging based on stimulated Raman scattering (SRS) microscopy represents a recent area of fruitful development that has been used to visualize cellular structures in three dimensions, with multiple spectroscopic colors at the nanometer scale. Several fundamental approaches to achieving super-resolution SRS imaging have been reported, including optical engineering strategies, expansion microscopy, deconvolution image analysis, and photoswitchable SRS reporters as methods to break the diffraction limit. These approaches have enabled the visualization of biological structures, cellular interactions, and dynamics with unprecedented detail. In this Perspective, an overview of the current strategies and capabilities for achieving super-resolution SRS imaging will be highlighted together with an outlook on potential directions of this rapidly evolving field.

超分辨率光学成像克服了光学显微镜的衍射极限,使以前看不到的样品内部分子细节变得可视化。基于受激拉曼散射(SRS)显微镜的超分辨率成像技术的实现代表了最近一个富有成果的发展领域,该技术已被用于在纳米尺度上以多种光谱颜色对细胞结构进行三维可视化。实现超分辨 SRS 成像的几种基本方法已有报道,包括光学工程策略、扩展显微镜、解卷积图像分析以及作为打破衍射极限方法的光开关 SRS 报告器。这些方法使生物结构、细胞相互作用和动态的可视化变得前所未有的详细。本视角将概述实现超分辨率 SRS 成像的当前策略和能力,并展望这一快速发展领域的潜在发展方向。
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引用次数: 0
Lipid Metabolic Heterogeneity during Early Embryogenesis Revealed by Hyper-3D Stimulated Raman Imaging
Pub Date : 2024-10-04 DOI: 10.1021/cbmi.4c0005510.1021/cbmi.4c00055
Jie Huang, Ling Zhang, Ninghui Shao, Yongqing Zhang, Yuyan Xu, Yihui Zhou, Delong Zhang, Jin Zhang* and Hyeon Jeong Lee*, 

Studying embryogenesis is fundamental to understanding developmental biology and reproductive medicine. Its process requires precise spatiotemporal regulations in which lipid metabolism plays a crucial role. However, the spatial dynamics of lipid species at the subcellular level remains obscure due to technical limitations. To address this challenge, we developed a hyperspectral 3D imaging and analysis method based on stimulated Raman scattering microscopy (hyper-3D SRS) to quantitatively assess lipid profiles in individual embryos through submicrometer resolution (xy), 3D optical sectioning (z), and chemical bond-selective (Ω) imaging. Using hyper-3D SRS, individual lipid droplets (LDs) in single cells were identified and quantified. Our findings revealed that the LD profiles within a single embryo are not uniform, even as early as the 2-cell stage. Notably, we also discovered a dynamic relationship between the LD size and unsaturation degree as embryos develop, indicating diverse lipid metabolism during early development. Furthermore, abnormal LDs were observed in oocytes of a progeria mouse model, suggesting that LDs could serve as a potential biomarker for assessing oocyte/embryo quality. Overall, our results highlight the potential of hyper-3D SRS as a noninvasive method for studying lipid content, composition, and subcellular distribution in embryos. This technique provides valuable insights into lipid metabolism during embryonic development and has the potential for clinical applications in evaluating oocyte/embryo quality.

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引用次数: 0
Lipid Metabolic Heterogeneity during Early Embryogenesis Revealed by Hyper-3D Stimulated Raman Imaging.
Pub Date : 2024-10-04 eCollection Date: 2025-01-27 DOI: 10.1021/cbmi.4c00055
Jie Huang, Ling Zhang, Ninghui Shao, Yongqing Zhang, Yuyan Xu, Yihui Zhou, Delong Zhang, Jin Zhang, Hyeon Jeong Lee

Studying embryogenesis is fundamental to understanding developmental biology and reproductive medicine. Its process requires precise spatiotemporal regulations in which lipid metabolism plays a crucial role. However, the spatial dynamics of lipid species at the subcellular level remains obscure due to technical limitations. To address this challenge, we developed a hyperspectral 3D imaging and analysis method based on stimulated Raman scattering microscopy (hyper-3D SRS) to quantitatively assess lipid profiles in individual embryos through submicrometer resolution (x-y), 3D optical sectioning (z), and chemical bond-selective (Ω) imaging. Using hyper-3D SRS, individual lipid droplets (LDs) in single cells were identified and quantified. Our findings revealed that the LD profiles within a single embryo are not uniform, even as early as the 2-cell stage. Notably, we also discovered a dynamic relationship between the LD size and unsaturation degree as embryos develop, indicating diverse lipid metabolism during early development. Furthermore, abnormal LDs were observed in oocytes of a progeria mouse model, suggesting that LDs could serve as a potential biomarker for assessing oocyte/embryo quality. Overall, our results highlight the potential of hyper-3D SRS as a noninvasive method for studying lipid content, composition, and subcellular distribution in embryos. This technique provides valuable insights into lipid metabolism during embryonic development and has the potential for clinical applications in evaluating oocyte/embryo quality.

{"title":"Lipid Metabolic Heterogeneity during Early Embryogenesis Revealed by Hyper-3D Stimulated Raman Imaging.","authors":"Jie Huang, Ling Zhang, Ninghui Shao, Yongqing Zhang, Yuyan Xu, Yihui Zhou, Delong Zhang, Jin Zhang, Hyeon Jeong Lee","doi":"10.1021/cbmi.4c00055","DOIUrl":"10.1021/cbmi.4c00055","url":null,"abstract":"<p><p>Studying embryogenesis is fundamental to understanding developmental biology and reproductive medicine. Its process requires precise spatiotemporal regulations in which lipid metabolism plays a crucial role. However, the spatial dynamics of lipid species at the subcellular level remains obscure due to technical limitations. To address this challenge, we developed a hyperspectral 3D imaging and analysis method based on stimulated Raman scattering microscopy (hyper-3D SRS) to quantitatively assess lipid profiles in individual embryos through submicrometer resolution (<i>x</i>-<i>y</i>), 3D optical sectioning (<i>z</i>), and chemical bond-selective (Ω) imaging. Using hyper-3D SRS, individual lipid droplets (LDs) in single cells were identified and quantified. Our findings revealed that the LD profiles within a single embryo are not uniform, even as early as the 2-cell stage. Notably, we also discovered a dynamic relationship between the LD size and unsaturation degree as embryos develop, indicating diverse lipid metabolism during early development. Furthermore, abnormal LDs were observed in oocytes of a progeria mouse model, suggesting that LDs could serve as a potential biomarker for assessing oocyte/embryo quality. Overall, our results highlight the potential of hyper-3D SRS as a noninvasive method for studying lipid content, composition, and subcellular distribution in embryos. This technique provides valuable insights into lipid metabolism during embryonic development and has the potential for clinical applications in evaluating oocyte/embryo quality.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"3 1","pages":"15-24"},"PeriodicalIF":0.0,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11775849/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143069418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Monitoring Macrophage Polarization with Gene Expression Reporters and Bioluminescence Phasor Analysis. 利用基因表达报告和生物发光相量分析监测巨噬细胞极化。
Pub Date : 2024-10-03 eCollection Date: 2024-11-25 DOI: 10.1021/cbmi.4c00049
Giulia Tedeschi, Mariana X Navarro, Lorenzo Scipioni, Tanvi K Sondhi, Jennifer A Prescher, Michelle A Digman

Macrophages exhibit a spectrum of behaviors upon activation and are generally classified as one of two types: inflammatory (M1) or anti-inflammatory (M2). Tracking these phenotypes in living cells can provide insight into immune function but remains a challenging pursuit. Existing methods are mostly limited to static readouts or are difficult to employ for multiplexed imaging in complex 3D environments while maintaining cellular resolution. We aimed to fill this void using bioluminescent technologies. Here we report genetically engineered luciferase reporters for the long-term monitoring of macrophage polarization via spectral phasor analysis. M1- and M2-specific promoters were used to drive the expression of bioluminescent enzymes in macrophage cell lines. The readouts were multiplexed and discernible in both 2D and 3D formats with single-cell resolution in living samples. Collectively, this work expands the toolbox of methods for monitoring macrophage polarization and provides a blueprint for monitoring other multifaceted networks in heterogeneous environments.

巨噬细胞在激活后表现出一系列行为,通常分为两种类型:炎症(M1)或抗炎(M2)。在活细胞中追踪这些表型可以提供对免疫功能的洞察,但仍然是一个具有挑战性的追求。现有的方法大多局限于静态读数,或者很难在复杂的3D环境中用于多路成像,同时保持细胞分辨率。我们的目标是用生物发光技术来填补这一空白。在这里,我们报告了通过光谱相量分析长期监测巨噬细胞极化的基因工程荧光素酶报告。使用M1和m2特异性启动子驱动巨噬细胞系中生物发光酶的表达。读数是多路复用和可识别的2D和3D格式与单细胞分辨率的活样本。总的来说,这项工作扩展了监测巨噬细胞极化的方法工具箱,并为在异质环境中监测其他多方面网络提供了蓝图。
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引用次数: 0
Monitoring Macrophage Polarization with Gene Expression Reporters and Bioluminescence Phasor Analysis 利用基因表达报告和生物发光相位分析监测巨噬细胞极化
Pub Date : 2024-10-03 DOI: 10.1021/cbmi.4c0004910.1021/cbmi.4c00049
Giulia Tedeschi, Mariana X. Navarro, Lorenzo Scipioni, Tanvi K. Sondhi, Jennifer A. Prescher* and Michelle A. Digman*, 

Macrophages exhibit a spectrum of behaviors upon activation and are generally classified as one of two types: inflammatory (M1) or anti-inflammatory (M2). Tracking these phenotypes in living cells can provide insight into immune function but remains a challenging pursuit. Existing methods are mostly limited to static readouts or are difficult to employ for multiplexed imaging in complex 3D environments while maintaining cellular resolution. We aimed to fill this void using bioluminescent technologies. Here we report genetically engineered luciferase reporters for the long-term monitoring of macrophage polarization via spectral phasor analysis. M1- and M2-specific promoters were used to drive the expression of bioluminescent enzymes in macrophage cell lines. The readouts were multiplexed and discernible in both 2D and 3D formats with single-cell resolution in living samples. Collectively, this work expands the toolbox of methods for monitoring macrophage polarization and provides a blueprint for monitoring other multifaceted networks in heterogeneous environments.

巨噬细胞在活化后会表现出一系列行为,一般分为两种类型:炎症(M1)或抗炎(M2)。在活细胞中跟踪这些表型可深入了解免疫功能,但这仍是一项具有挑战性的工作。现有的方法大多局限于静态读数,或者难以在复杂的三维环境中进行多重成像,同时保持细胞分辨率。我们的目标是利用生物发光技术填补这一空白。在此,我们报告了基因工程荧光素酶报告器,用于通过光谱相位分析长期监测巨噬细胞极化。我们使用 M1 和 M2 特异性启动子来驱动巨噬细胞系中生物发光酶的表达。在活体样本中,读出的结果是多路复用的,并可在二维和三维格式中以单细胞分辨率辨别。总之,这项工作扩展了监测巨噬细胞极化的方法工具箱,并为监测异质环境中的其他多层面网络提供了蓝图。
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引用次数: 0
Rapid and Large-Scale Synthesis of Chiral and Fluorescent Sulfur Quantum Dots for Intracellular Temperature Monitoring 用于胞内温度监测的手性荧光硫量子点的快速大规模合成
Pub Date : 2024-09-20 DOI: 10.1021/cbmi.4c0005210.1021/cbmi.4c00052
Li Zhao, Tianjian Sha, Yufu Liu, Qingsong Mei*, Haibin Li, Pinghua Sun, Haibo Zhou* and Huaihong Cai*, 

The large-scale preparation of fluorescent nanomaterials with laboratory-relevant chemical and optical properties will greatly forward their consumer market applications; however, it still remains challenging. In this work, a universal strategy was developed for the rapid and large-scale synthesis of fluorescent sulfur quantum dots that recently has drawn great attention because of their unique optical characteristics. From the fact that empty 3d orbitals of sulfide species are able to bind with lone-pair π electrons of the heteroatomic groups, many amino-group containing compounds, such as amino acid and polyethylenimine molecules, were exploited to synthesize sulfur quantum dots. This 10 min preparation period endowed sulfur quantum dots with bright blue fluorescence and also chirality. Due to the user-friendly and rapid operation, this strategy can be extended to the large-scale synthesis of sulfur quantum dots with a yield of 16.844 g for one batch of experiment. Moreover, it was found that the sulfur quantum dots exhibited a reversible temperature-dependent luminescent property with a sensitivity of 0.72%/°C, which showed excellent intracellular temperature monitoring capability for inflammation-related disease diagnostics.

具有实验室相关化学和光学性质的荧光纳米材料的大规模制备将极大地促进其消费市场应用;然而,它仍然具有挑战性。本文提出了一种快速、大规模合成荧光硫量子点的通用策略,该方法因其独特的光学特性而受到广泛关注。由于硫化物的空三维轨道能够与杂原子基团的孤对π电子结合,许多含氨基的化合物,如氨基酸和聚乙烯亚胺分子,被用来合成硫量子点。这10 min的制备时间使硫量子点具有明亮的蓝色荧光和手性。该策略操作简便,操作快捷,可推广到硫量子点的大规模合成,一次实验产率可达16.844 g。此外,研究发现,硫量子点具有可逆的温度依赖性发光特性,灵敏度为0.72%/°C,具有良好的细胞内温度监测能力,可用于炎症相关疾病的诊断。
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引用次数: 0
Rapid and Large-Scale Synthesis of Chiral and Fluorescent Sulfur Quantum Dots for Intracellular Temperature Monitoring. 用于胞内温度监测的手性荧光硫量子点的快速大规模合成。
Pub Date : 2024-09-20 eCollection Date: 2024-12-23 DOI: 10.1021/cbmi.4c00052
Li Zhao, Tianjian Sha, Yufu Liu, Qingsong Mei, Haibin Li, Pinghua Sun, Haibo Zhou, Huaihong Cai

The large-scale preparation of fluorescent nanomaterials with laboratory-relevant chemical and optical properties will greatly forward their consumer market applications; however, it still remains challenging. In this work, a universal strategy was developed for the rapid and large-scale synthesis of fluorescent sulfur quantum dots that recently has drawn great attention because of their unique optical characteristics. From the fact that empty 3d orbitals of sulfide species are able to bind with lone-pair π electrons of the heteroatomic groups, many amino-group containing compounds, such as amino acid and polyethylenimine molecules, were exploited to synthesize sulfur quantum dots. This 10 min preparation period endowed sulfur quantum dots with bright blue fluorescence and also chirality. Due to the user-friendly and rapid operation, this strategy can be extended to the large-scale synthesis of sulfur quantum dots with a yield of 16.844 g for one batch of experiment. Moreover, it was found that the sulfur quantum dots exhibited a reversible temperature-dependent luminescent property with a sensitivity of 0.72%/°C, which showed excellent intracellular temperature monitoring capability for inflammation-related disease diagnostics.

具有实验室相关化学和光学性质的荧光纳米材料的大规模制备将极大地促进其消费市场应用;然而,它仍然具有挑战性。本文提出了一种快速、大规模合成荧光硫量子点的通用策略,该方法因其独特的光学特性而受到广泛关注。由于硫化物的空三维轨道能够与杂原子基团的孤对π电子结合,许多含氨基的化合物,如氨基酸和聚乙烯亚胺分子,被用来合成硫量子点。这10 min的制备时间使硫量子点具有明亮的蓝色荧光和手性。该策略操作简便,操作快捷,可推广到硫量子点的大规模合成,一次实验产率可达16.844 g。此外,研究发现,硫量子点具有可逆的温度依赖性发光特性,灵敏度为0.72%/°C,具有良好的细胞内温度监测能力,可用于炎症相关疾病的诊断。
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引用次数: 0
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Chemical & Biomedical Imaging
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