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Antibacterial Effect of Carvacrol and Coconut Oil on Selected Pathogenic Bacteria 香芹酚和椰子油对部分病原菌的抗菌作用
Q3 Agricultural and Biological Sciences Pub Date : 2018-03-01 DOI: 10.2478/sab-2018-0008
M. Božík, P. Hovorková, P. Klouček
Abstract Essential oils play a prominent role as flavouring agents and fragrances in the food and perfume industries. Carvacrol is a major component of various essential oils, such as oregano and thyme oils, and is responsible for their antimicrobial activity. Lauric acid is a medium-chain fatty acid (MCFA) with a high antibacterial potential. Both carvacrol and MCFAs have been used empirically as antimicrobial agents. Here, we tested the inhibitory properties of carvacrol and coconut (Cocos nucifera L.) oil containing a high percentage of MCFAs against 5 harmful bacterial pathogens: Escherichia coli, Salmonella Enteritidis, Staphylococcus aureus, Listeria monocytogenes, and Enterococcus cecorum. Gas chromatography (GC-FID) analysis of coconut oil showed a high concentration of lauric acid (41%). Microdilution antimicrobial assays showed that the combination of carvacrol and coconut oil had a stronger antibacterial effect against all tested bacteria than both agents separately. We conclude that carvacrol could significantly improve the antibacterial effect of coconut oil.
摘要精油在食品和香水行业中作为调味剂和香料发挥着重要作用。Carvacrol是各种精油的主要成分,如牛至油和百里香油,并对其抗菌活性负责。月桂酸是一种中链脂肪酸,具有很高的抗菌潜力。香芹酚和MCFA都已被用作抗菌剂。在这里,我们测试了含有高百分比MCFA的香芹酚和椰子油对5种有害细菌病原体的抑制特性:大肠杆菌、肠炎沙门氏菌、金黄色葡萄球菌、单核细胞增多性李斯特菌和盲肠肠球菌。椰子油的气相色谱(GC-FID)分析显示高浓度的月桂酸(41%)。微量稀释抗菌试验表明,香芹酚和椰子油的组合对所有测试细菌的抗菌效果都比单独使用两种药物更强。我们得出结论,香芹酚可以显著提高椰子油的抗菌效果。
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引用次数: 7
Production and Commercialization of Timber in Angola after the Declaration of Independence 《独立宣言》后安哥拉木材的生产和商业化
Q3 Agricultural and Biological Sciences Pub Date : 2018-03-01 DOI: 10.2478/sab-2018-0007
V. Chiteculo, M. Hájek, P. Kubová
Abstract The policy of production and commercialization of timber before and after the independence of Angola was assessed. Historical production of timber under control of Portuguese settlers before 1975 in comparison to the production of timber after this period was reviewed. We used a combination of published scientific studies and government reports to support the background of the paper and a structured questionnaire survey from which analyses were drawn using a logistic regression model. It was found out that timber production declined dramatically after Angola gained independence; the production of logs dropped from 555 000 m3 in 1973 to less than 115 400 m3. Out of the 100 mills that had existed in Angola before 1975 only twenty have been in operation today with annual wood production of less than 20% of extraction capacity. The knowledge concerning the historical production of timber before and after 1975 is not sufficient to provide suggestions for a management plan on what trees, where, and when are to be cut.
摘要对安哥拉独立前后的木材生产和商业化政策进行了评估。回顾了1975年之前葡萄牙定居者控制下的木材生产与这一时期之后的木材生产的历史对比。我们结合已发表的科学研究和政府报告来支持论文的背景,并采用结构化问卷调查,使用逻辑回归模型进行分析。研究发现,安哥拉独立后,木材产量急剧下降;原木产量从1973年的55.5万立方米下降到不足11.5万立方米。在安哥拉1975年之前存在的100家工厂中,只有20家在运营,年木材产量不到开采能力的20%。关于1975年前后木材历史生产的知识不足以为砍伐哪些树木、在哪里砍伐以及何时砍伐的管理计划提供建议。
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引用次数: 5
Ecotoxicity of Wastewater from Medical Facilities: A Review 医疗设施废水的生态毒性研究进展
Q3 Agricultural and Biological Sciences Pub Date : 2018-03-01 DOI: 10.2478/sab-2018-0005
A. Cidlinová, Z. Wittlingerová, M. Zimová, T. Chrobakova, A. Petruželková
Abstract Wastewater from medical facilities contains a wide range of chemicals (in particular pharmaceuticals, disinfectants, heavy metals, contrast media, and radionuclides) and pathogens, therefore it constitutes a risk to the environment and human health. Many micropollutants are not efficiently eliminated during wastewater treatment and contaminate both surface water and groundwater. As we lack information about the long-term effects of low concentrations of micropollutants in the aquatic environment, it is not possible to rule out their adverse effects on aquatic organisms and human health. It is, therefore, necessary to focus on the evaluation of chronic toxicity in particular when assessing the environmental and health risks and to develop standards for the regulation of hazardous substances in wastewater from medical facilities on the basis of collected data. Wastewater from medical facilities is a complex mixture of many compounds that may have synergetic, antagonistic or additive effects on organisms. To evaluate the influence of a wide range of pollutants contained in the effluents from medical facilities on aquatic ecosystems, it is necessary to determine their ecotoxicity.
摘要医疗设施废水含有多种化学物质(特别是药品、消毒剂、重金属、造影剂和放射性核素)和病原体,因此对环境和人类健康构成风险。许多微污染物在废水处理过程中没有得到有效消除,并污染地表水和地下水。由于我们缺乏关于低浓度微污染物对水生环境的长期影响的信息,因此不可能排除它们对水生生物和人类健康的不利影响。因此,有必要重点评估慢性毒性,特别是在评估环境和健康风险时,并根据收集的数据制定医疗设施废水中有害物质的监管标准。医疗设施废水是多种化合物的复杂混合物,可能对生物体产生协同、拮抗或相加作用。为了评估医疗设施废水中所含的各种污染物对水生生态系统的影响,有必要确定其生态毒性。
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引用次数: 6
Phenolic Compounds in Wetland Macrophytes 湿地大型植物中的酚类化合物
Q3 Agricultural and Biological Sciences Pub Date : 2018-03-01 DOI: 10.2478/sab-2018-0001
T. Březinová, J. Vymazal
Abstract Phenolics are the most widely distributed class of plant secondary metabolites and higher plants are able to produce several thousand different phenolic compounds. It has been reported that phenolics are more resistant to decomposition due to the negative impact on the microorganisms involved. Therefore, it may be hypothesized that the higher content of phenolics in plant tissue may cause slower decomposition rates and potentially higher carbon sequestration in the soil. The primary goal of this study was to evaluate and compare the content of phenolics in seven common macrophytes in the Czech Republic. Aboveground biomass was sampled in June 2016 at seven different localities and phenolics concentrations determined by spectrophotometric methods according to the Folin-Cioacalteau method ranged from 9.02 to 28.39 g kg−1 dry biomass weight (DW). Several plants were also harvested in August, October and December in order to follow a seasonal pattern. According to the results it seems that surveyed macrophytes vary widely in phenolics concentrations in relation to sampled site, harvesting time, plant species, and plant parts as well.
酚类物质是植物次生代谢产物中分布最广泛的一类,高等植物能够产生数千种不同的酚类化合物。据报道,由于对相关微生物的负面影响,酚类物质更不易分解。因此,可以假设植物组织中酚类物质含量较高可能会导致土壤中分解速度较慢和潜在的碳固存量较高。本研究的主要目的是评估和比较捷克共和国七种常见大型植物中酚类物质的含量。2016年6月,在7个不同的地点采集了地上生物量,根据Folin-Cioacalteau法分光光度法测定的酚类物质浓度为9.02 ~ 28.39 g kg−1干生物量(DW)。为了遵循季节性模式,一些植物也在8月、10月和12月收获。结果表明,所调查的大型植物中酚类物质的含量与取样地点、采收时间、植物种类和植物部位有关。
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引用次数: 12
Parasites of Myocastor coypus – a Comparison in Farm Animals and Their Feral Counterparts 农场动物与野生动物的肌瘤性瓢虫寄生虫比较
Q3 Agricultural and Biological Sciences Pub Date : 2018-03-01 DOI: 10.2478/sab-2018-0004
S. Nechybová, I. Langrová, E. Tůmová
Abstract The parasitic status in the coypus (nutrias) Myocastor coypus, both farm-bred or free-living on river banks in the Czech Republic, was determined. Faecal samples were collected from 200 coypus originating from 11 farms (farm-bred animals) and from 20 individuals living at 14 natural localities (feral animals). Faeces were examined for nematode eggs and coccidian oocysts using the McMaster method. The evaluation of faeces from farm-bred coypus indicated infection with the following parasites: Trichuris sp., Strongyloides sp., Trichostrongylus sp., Eimeria seidelli, Eimeria nutriae, Eimeria coypi, and Eimeria myopotami. Free-living feral rodents harboured Eimeria nutriae, E. coypi, Strongyloides sp. and in one case also Trichuris sp. An additional visceral examination of 20 coypus originating from five farms revealed two nematode species in the gastrointestinal tracts, namely Trichuris myocastoris and Strongyloides myopotami. The study indicated that feral coypus are far less parasitized than their captive counterparts.
摘要测定了在捷克共和国农场饲养或在河岸自由生活的郊狼(nutrias)的寄生状况。粪便样本采集自来自11个农场(农场饲养的动物)的200只郊狼和生活在14个自然地点的20只个体(野生动物)。用麦克马斯特法检测粪便中的线虫卵和球虫卵囊。对农场养殖的郊狼粪便的评估表明,其感染了以下寄生虫:Tricuris sp.、Strongyloides sp.、Trichostrongylus sp.、Eimeria seidelli、Eimeria-nuturiae、Eimerian-coypi和Eimeria myotomi。自由生活的野生啮齿动物携带营养艾美耳球虫(Eimeria nutriae)、E.coypi、类强线虫(Strongyloides sp。研究表明,野生郊狼的寄生率远低于圈养的同类。
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引用次数: 1
Exploitation of Hazelnut (Corylus avellana) Shell Waste in the Form of Polymer–Particle Biocomposite 以聚合物-颗粒生物复合材料的形式开发榛子壳废弃物
Q3 Agricultural and Biological Sciences Pub Date : 2018-03-01 DOI: 10.2478/sab-2018-0009
M. Müller, P. Valášek, M. Linda, S. Petrásek
Abstract Mechanically ground hazelnut (Corylus avellana) shells, a food industry by-product of hazelnuts processing, were tested for use as a composite material filler. Mechanical properties and fracture surface of the composite were evaluated using scanning electron microscopy. Polymer composites, i.e. resins filled with microparticles of hazelnut shells, were tested at various concentrations of the filler (5, 10, 20, 30, and 40 wt%). Hazelnut shell microparticles used at low concentration (5 wt%) increased tensile strength. The filler did not considerably influence hardness of the composite. Adhesive bond strength did not significantly change up to 20 wt%. The hazelnut shell microparticles were well wetted with the resin.
摘要对榛子加工过程中的食品工业副产品——机械研磨榛子壳(Corylus avellana)作为复合材料填料进行了试验。用扫描电子显微镜对复合材料的力学性能和断裂表面进行了评价。聚合物复合材料,即填充有榛子壳微粒的树脂,在不同浓度的填料(5、10、20、30和40wt%)下进行测试。以低浓度(5wt%)使用的榛子壳微粒提高了拉伸强度。填料对复合材料的硬度没有显著影响。粘合强度在高达20wt%时没有显著变化。榛子壳微粒用树脂充分润湿。
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引用次数: 8
Importance of Food Selection for Celiac Sprue Disease 食物选择对乳糜泻的重要性
Q3 Agricultural and Biological Sciences Pub Date : 2017-12-20 DOI: 10.1515/sab-2017-0027
O. Famera, I. Halova, L. Kouřimská
Abstract A questionnaire survey was conducted among people with celiac disease. Questions were focused on dietary foods available and the preference of suitable grain food species. A one-week diet menu was prepared for the participants of the survey. Daily intake of major nutrients and energy was determined. Supermarkets have the largest selection of dietary foods, although in a narrow range. Celiac patients often rely on themselves to make and prepare food. The majority of respondents (57%) considered dietary restrictions as burdening; 43% have adapted to this situation. Food made of rice was the most popular among cereal foods for 50% of respondents, corn for 39%, and buckwheat and millet for only 7% of respondents. Nobody classified amaranth as the most popular food. The model diet for people with celiac disease exceeded the recommended daily intake of protein and undervalued lipid ingestion. This problem was mainly caused by the food composition of the evening meal. Total daily energy intake of 9900 kJ met the women’s needs. There were still 2100 kJ missing for men. The terms ‘gluten-free food or diet’ and ‘gliadin content’, which generally relate to the products of wheat, rye, oats, and triticale, are not accurate.
摘要对乳糜泻患者进行问卷调查。问题集中在可获得的膳食食物和对合适的粮食食物种类的偏好上。研究人员为调查参与者准备了一份为期一周的饮食菜单。确定了每日主要营养素和能量的摄入量。超市有最多的膳食食品选择,尽管范围很窄。乳糜泻患者经常依靠自己制作和准备食物。大多数答复者(57%)认为饮食限制是负担;43%的人已经适应了这种情况。在谷类食品中,大米制成的食品最受欢迎,占50%,玉米占39%,荞麦和小米仅占7%。没有人把苋菜列为最受欢迎的食物。乳糜泻患者的模型饮食超过了推荐的每日蛋白质摄入量,并低估了脂质摄入量。这个问题主要是由晚餐的食物成分引起的。每天摄入的总能量为9900千焦,满足了女性的需要。男性仍然少了2100千焦。术语“无麸质食品或饮食”和“麦胶蛋白含量”通常与小麦、黑麦、燕麦和小黑麦的产品有关,这是不准确的。
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引用次数: 0
Antimicrobial Potential of Probiotics in Combination with Start er Lactic Acid Bacteria 益生菌与起始乳酸菌联合应用的抗菌潜力
Q3 Agricultural and Biological Sciences Pub Date : 2017-12-20 DOI: 10.1515/sab-2017-0028
Tatiana Mančušková, A. Medveďová, Ľ. Valík
Abstract The symbiotic interrelationship of probiotic bacteria and starter lactic acid bacteria is of fundamental importance in many fields of industrial microbiology and it is also a great interest of food technologists. This study deals with the antimicrobial potential of cell-free supernatants of Lactobacillus acidophilus NCFM, L. rhamnosus GG and Fresco culture when cultivated alone or together against 5 strains of Escherichia coli and 5 strains of Staphylococcus aureus. While the effect on E. coli was not proven (average change of final density compared to the control was only ΔNEC,24h = 0.12 log CFU ml-1), the decrease of S. aureus final density in the presence of nisin and cell-free supernatant of L. acidophilus NCFM and Fresco culture was considerable (ΔNSA,24h,nis10 = -1.95 log CFU ml-1 and ΔNSA,24h,NCFM+Fr24 = -0.69 log CFU ml-1, respectively).
摘要益生菌和发酵乳酸菌的共生关系在工业微生物学的许多领域都具有重要意义,也是食品技术人员的极大兴趣。本研究研究了嗜酸乳杆菌NCFM、鼠李糖乳杆菌GG和Fresco培养物的无细胞上清液单独或联合培养时对5株大肠杆菌和5株金黄色葡萄球菌的抗菌潜力。虽然对大肠杆菌的影响尚未得到证实(与对照相比,最终密度的平均变化仅为ΔNEC,24h=0.12 log CFU ml-1),但在乳酸链球菌和嗜酸乳杆菌NCFM和Fresco培养物的无细胞上清液存在的情况下,金黄色葡萄球菌的最终密度显著降低(ΔNSA,24h,nis10=-1.95 log CFU ml-1和ΔNSA、24h,NCFM+Fr24=-0.69 log CFU ml-1,分别为)。
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引用次数: 3
Does seed modification and nitrogen addition affect seed germination of Pulsatilla grandis? 种子改良和施氮对白头翁种子发芽有影响吗?
Q3 Agricultural and Biological Sciences Pub Date : 2017-12-20 DOI: 10.1515/sab-2017-0029
M. Bochenková, Petr Karlík, Michal Hejcman, P. Jiras
Abstract Pulsatilla grandis is an endangered species in the Czech Republic and is protected in whole Europe because the number of its populations is declining. One of the possible causes is the deposition of atmospheric nitrogen. In our research, we investigated how nitrogen concentrations and seed appendage removal directly affect the species’ seed germination.Seeds were allowed to germinate under laboratory conditions in water solutions of NH4NO3 ranging in concentration from 0 to 4239 mg N l-1. They were able to germinate up to the concentration of 848 mg N l-1 even when covered with mycelium, which supports the idea that they can tolerate being strongly infected by fungi. We also found a significant positive effect of seed appendage removal on seed germination. Seeds without appendages germinated, on the average, with 11% greater probability, compared to seeds with appendages. We conclude that the germination of P. grandis is not directly affected by high N concentrations in rain water, which can range from 10 to 13 mg N l-1 near large cities. Surprisingly, low concentrations of N (up to 34 mg N l-1) might even slightly support the seed germination of P. grandis. The negative effect of N deposition on seeds is indirect and acts in conjunction with the absence of management at localities.
摘要白头翁在捷克共和国是一种濒危物种,由于其种群数量正在下降,整个欧洲都受到保护。其中一个可能的原因是大气中氮的沉积。在我们的研究中,我们调查了氮浓度和种子附属物的去除如何直接影响物种的种子发芽。允许种子在实验室条件下在浓度为0至4239mg N-1的NH4NO3水溶液中发芽。即使被菌丝体覆盖,它们也能发芽至848 mg N-1的浓度,这支持了它们可以耐受真菌强烈感染的观点。我们还发现去除种子附属物对种子发芽有显著的积极影响。与有附属物的种子相比,没有附属物的种子发芽的概率平均高出11%。我们得出的结论是,大城市附近雨水中的高氮浓度(10至13mg N-1)不会直接影响大白菜的发芽。令人惊讶的是,低浓度的N(高达34mg N l-1)甚至可能略微支持P.grandis的种子发芽。氮沉降对种子的负面影响是间接的,与当地缺乏管理有关。
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引用次数: 4
Effect of Different Organic Wastes on Soil Propertie s and Plant Growth and Yield: a Review 不同有机废弃物对土壤性质及植物生长和产量的影响
Q3 Agricultural and Biological Sciences Pub Date : 2017-12-20 DOI: 10.1515/sab-2017-0030
M. Z. Hossain, P. Fragstein, P. Niemsdorff, J. Heß
Abstract The use of organic wastes in agriculture plays a great role in recycling essential plant nutrients, sustaining soil security as well as protecting the environment from unwanted hazards. This review article deals with the effect of different kinds of organic wastes on soil properties and plant growth and yield. Municipal solid waste is mainly used as a source of nitrogen and organic matter, improving soil properties and microbial activity that are closely related to soil fertility. Biowaste and food waste increase pH, nitrogen content, cation exchange capacity, water holding capacity, and microbial biomass in soil. Sewage sludge contains various amounts of organic matter and huge amounts of plant nutrients. Manure is a common waste which improves soil properties by adding nutrients and increases microbial and enzyme activity in soil. It also reduces toxicity of some heavy metals. These organic wastes have a great positive impact on soil physical, chemical, and biological properties as well as stimulate plant growth and thus increase the yield of crops.
农业有机废弃物的利用在回收植物必需养分、维持土壤安全以及保护环境免受有害危害方面发挥着重要作用。本文综述了不同种类的有机废弃物对土壤性质、植物生长和产量的影响。城市生活垃圾主要作为氮和有机质的来源,改善与土壤肥力密切相关的土壤性质和微生物活性。生物垃圾和食物垃圾增加了土壤的pH值、氮含量、阳离子交换能力、持水量和微生物生物量。污水污泥中含有不同数量的有机物和大量的植物营养物质。粪肥是一种常见的废物,它通过增加养分和增加土壤中的微生物和酶的活性来改善土壤的性质。它还能降低一些重金属的毒性。这些有机废物对土壤的物理、化学和生物特性有很大的积极影响,并刺激植物生长,从而提高作物产量。
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引用次数: 57
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Scientia Agriculturae Bohemica
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