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Toxicity and Biochemical Effects of Cumin and Basil Essential Oils on Tribolium Castaneum 孜然和罗勒精油对蓖麻的毒性及生化作用
Q3 Agricultural and Biological Sciences Pub Date : 2021-09-01 DOI: 10.2478/sab-2021-0005
A. F. Omar, M. El-Ebiary, G. Nasr, H. M. Hassan
Abstract The essential oils (EOs) of cumin (Cuminum cyminum L.) seeds and basil (Ocimum basilicum L.) herb were extracted by hydrodistillation and tested against the red flour beetle, Tribolium castaneum (Herbst) for insecticidal and biochemical effects on certain enzymes of this insect. Major components of C. cyminum EO determined by gass chromatography-mass spectrometry (GC/MS) analysis were γ-terpinene (15.78 %) and benzenemethanol (11.32 %), while those of O. basilicum EO were linalool (56.7 %) and epi-α-cadinol (11.4 %). The lethal concentration values for 50% mortality after three days of T. castaneum whole body exposure were 678 mg kg–1 for cumin oil and 755 mg kg–1 for basil oil. The enzymatic activity of treated insects showed a reduction in total protein, alkaline phosphatase, aspartate aminotransferase, and alanine aminotransferase compared to untreated ones. However, α-amylase activity increased with both tested EOs. Hence, for T. castaneum control, these EOs may represent alternatives to conventional insecticides.
摘要采用加氢蒸馏法提取孜然(Cuminum cyminum L.)种子和罗勒(Ocimum basilicum L.)草本植物精油,并对红粉甲虫Tribolium castaneum (Herbst)进行杀虫和生化作用试验。气相色谱-质谱联用(GC/MS)分析发现,香薷精油的主要成分为γ-萜烯(15.78%)和苯甲醇(11.32%),香樟精油的主要成分为芳樟醇(56.7%)和外皮-α-二醇(11.4%)。茴香油和罗勒油分别为678 mg kg-1和755 mg kg-1。与未处理的昆虫相比,处理过的昆虫的总蛋白、碱性磷酸酶、天冬氨酸转氨酶和丙氨酸转氨酶活性降低。α-淀粉酶活性随两种EOs的增加而增加。因此,对于玉米赤霉的控制,这些EOs可能是传统杀虫剂的替代品。
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引用次数: 3
Plant Extracts Inducing Enzyme Activity in Grains Against Loose Smut Disease 植物提取物诱导谷物抗疏松性Smut病的酶活性
Q3 Agricultural and Biological Sciences Pub Date : 2021-09-01 DOI: 10.2478/sab-2021-0006
B. Karsou, R. Samara
Abstract This study investigated the role of endogenous Palestinian plant extracts in inducing wheat and barley resistance systems against loose smut disease with the aim to alternate the chemical pest control with natural fungicides. Twenty indigenous herbal plant extracts and essential oils were assessed for their biological and antifungal properties against Ustilago tritici and Ustilago nuda. Their potential role in inducing resistance pathways was studied on four different cultivars of wheat and barley. Two common enzyme indicators – guaiacol peroxidase (POX) and polyphenol oxidase (PPO) – are expressed in plants only after physical or chemical induction. The antifungal activity of the plant extracts was investigated in vitro. Totally 70 % of the plant extracts showed antifungal activity against Ustilago tritici and Ustilago nuda. Coridothyme extracts ranked first (61 %) in the fungal growth inhibition, followed by varthemia, salvia, ambrosia, artemisia, and lemon thyme. Some plant extracts significantly increased the POX and PPO effect compared to control for all the wheat and barley cultivars tested. The study revealed that oregano, clove or lavender and pomegranate, common yarrow or chamomile oil effectively induced the resistance indicator enzymes in wheat and barley.
摘要本研究研究了巴勒斯坦内源植物提取物在诱导小麦和大麦抗松曲病系统中的作用,旨在将化学害虫防治与天然杀菌剂交替使用。对20种本土草本植物提取物和精油的生物学特性和抗真菌特性进行了评估,以对抗黑曲霉和黑曲霉。在四个不同的小麦和大麦品种上研究了它们在诱导抗性途径中的潜在作用。两种常见的酶指标——愈创木酚过氧化物酶(POX)和多酚氧化酶(PPO)——只有在物理或化学诱导后才能在植物中表达。研究了植物提取物的体外抗真菌活性。70%的植物提取物对小麦黑粉和裸黑粉具有抗真菌活性。Coridothyme提取物对真菌生长的抑制作用排名第一(61%),其次是varthemia、丹参、氨溴香、蒿属和柠檬百里香。对于所有测试的小麦和大麦品种,与对照相比,一些植物提取物显著增加了POX和PPO效应。研究表明,牛至、丁香或薰衣草、石榴、普通庭院或洋甘菊油有效地诱导了小麦和大麦的抗性指示酶。
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引用次数: 1
In Vitro Assessment of N-Phenyl Imides in the Management of Meloidogyne Incognita N-苯基酰亚胺在根结线虫治理中的体外评价
Q3 Agricultural and Biological Sciences Pub Date : 2021-09-01 DOI: 10.2478/sab-2021-0007
O. Fabiyi, A. Claudius-Cole, G. Olatunji
Abstract The infestation with root knot nematode Meloidogyne spp. is a key issue in agriculture. Conventional control methods are based on the use of synthetic nematicides, which comes with severe environmental problems. In this study, n-phenyl imide and n-phenyl phthalamic acid were synthesized and reacted independently with Enantia chlorantha crude extract–manganese chloride complex. The effects of the resulting organic compounds were appraised against the root knot nematode Meloidogyne incognita (Kofoid and White 1919) juveniles and eggs in two laboratory experiments. The most active compound was n-phenyl phthalamic acid (PN/TLMA) with 4% egg hatch over a 9-day observation after treatment as against distilled water which recorded 100% egg hatch at 9 days after treatment. n-Phenyl phthalamic acid showed 100% juvenile mortality at 10 days of observation compared to carbofuran dissolved in water (CBFN/water) and carbofuran dissolved in hydroxypropyl-β-cyclodextrin (CBFN/HPCD) while no outstanding (P < 0.05) difference was recorded between the effects of other organic compounds and carbofuran in both solvents. The different rates of treatment applications were not appreciably (P<0.05) dissimilar on percentage juvenile mortality and egg hatch. The nematicidal test results indicated that the synthesized imide compounds with manganese complex moiety are a promising basis for developing new nematicidal compounds with less environmental hazard.
摘要根结线虫根结线虫的危害是农业中的一个关键问题。传统的控制方法是基于使用合成杀线虫剂,这会带来严重的环境问题。在本研究中,合成了正苯基酰亚胺和正苯基邻苯二甲酸,并与氯兰香粗提取物-氯化锰络合物独立反应。在两个实验室实验中评估了所得有机化合物对根结线虫南方根结线虫(Kofoid和White 1919)幼虫和卵的效果。最具活性的化合物是n-苯基邻苯二甲酸(PN/TLMA),在处理后的9天观察中孵化率为4%,而蒸馏水在处理后9天孵化率为100%。与溶解在水中的呋喃丹(CBFN/水)和溶解在羟丙基-β-环糊精中的呋喃丹相比,n-苯基邻苯二甲酸在观察10天时显示出100%的青少年死亡率,而其他有机化合物和呋喃丹在两种溶剂中的效果之间没有显著差异(P<0.05)。不同的处理率在幼崽死亡率和孵化率方面没有明显差异(P<0.05)。杀线虫试验结果表明,合成的含锰配合物的酰亚胺化合物为开发环境危害较小的新型杀线虫化合物奠定了良好的基础。
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引用次数: 2
Response of Laying Hens to Aqueous Extracts of Petiveria alliacea Root and Leaf 蛋鸡对韭菜根和叶水提物的反应
Q3 Agricultural and Biological Sciences Pub Date : 2021-06-01 DOI: 10.2478/sab-2021-0004
A. M. Oyeleke, O. Adeyemi, L. T. Egbeyale, R. Sobayo, R. Olaifa
Abstract This study investigated the response of laying hens to aqueous extracts of Petiveria alliacea root and leaf. A total of 288 eighteen-week-old Isa brown pullets were used for the 25-week study. The pullets were arranged in a 2 × 4 factorial experimental layout in a completely randomized design. The pullets were distributed into two groups administered root extract or leaf extract. Pullets in each group were allotted to four subgroups administered aqueous extracts of Petiveria alliacea at 15, 30 and 45 g l–1 concentration levels making eight treatments in total. Each treatment was replicated three times with twelve pullets per replicate. Eimeria oocyst counts and intestinal bacteria counts were lower (P < 0.0001 and P = 0.0028, respectively) in hens administered 15, 30 and 45 g l–1 of Petiveria alliacea extracts than the control. The highest (P < 0.0001) antibody titre against Newcastle disease vaccine was recorded in hens administered 30 and 45 g l–1 concentrations of root (9.06 and 9.10 log2, respectively) and leaf (9.08 and 9.18 log2, respectively) extracts. The liver sections of hens in all treatments appeared normal. In conclusion, aqueous extract of Petiveria alliacea root and leaf at 30 and 45 g l–1 concentrations performed best as antimicrobial and immune stimulating agent without impairing liver health.
本文研究了蛋鸡对大蒜根和叶水提取物的反应。共有288只18周大的Isa棕色幼犬用于25周的研究。以2×4析因实验布局,采用完全随机设计。将幼犬分为两组,分别给予根提取物或叶提取物。每组的幼犬被分为四个亚组,分别以15、30和45 g l–1的浓度水平施用大蒜水提取物,共进行八次治疗。每种处理重复三次,每次重复12只幼崽。与对照组相比,施用15、30和45 g l–1大蒜提取物的母鸡的艾美耳球虫卵囊计数和肠道细菌计数较低(分别为P<0.0001和P=0.0028)。接种30和45 g l–1浓度的根(分别为9.06和9.10 log2)和叶(分别为90.8和9.18 log2)提取物的母鸡对新城疫疫苗的抗体滴度最高(P<0.0001)。所有处理的母鸡肝脏切片均正常。总之,在不损害肝脏健康的情况下,30和45 g l–1浓度的大蒜根和叶的水提取物作为抗菌和免疫刺激剂表现最佳。
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引用次数: 0
Lacticaseibacilli and Lactococci from Slovak Raw Goat Milk and their Potential 斯洛伐克生羊奶中的乳杆菌和乳球菌及其潜力
Q3 Agricultural and Biological Sciences Pub Date : 2021-06-01 DOI: 10.2478/sab-2021-0003
A. Lauková, M. Simonová, M. Tomáška, M. Kološta, M. Drončovský, E. Dvorožňáková
Abstract Strains potential such as bacteriocin activity, biofilm formation ability, growth in skim milk, susceptibility to antibiotics, tolerance to bile and low pH as well as enzyme production was tested in the species Lacticaseibacillus paracasei and Lactococcus lactis detected in Slovak raw goat milk. The strains showed mostly low-grade biofilm formation ability, susceptibility to antibiotics and sufficient tolerance to oxgall/bile. Lacticaseibacillus (Lcb.) paracasei ZM-1, ZM-2 and Lactococcus (Lc.) lactis PD MO 1/8 showed high tolerance to pH 3 (67 %, 83 % and 63 %, respectively). The strains showed bacteriocin activity against the principal indicator Enterococcus avium EA5 (inhibition zone ranging 5–24 mm). A concentrated substance of Lcb. paracasei LPa ML 12/1 (pH 6.3) inhibited EA5 strain (inhibition activity 100 AU ml–1). Lcb. paracasei ZM-1 and ZM-2 also produced a high amount of β-galactosidase (40 nmol). Although the strains indicated their beneficial potential, additional testing is needed; some tests are in processing for further possible application of selected strains in dairy.
摘要在斯洛伐克生羊奶中检测到的副干酪乳杆菌和乳酸乳球菌中,对菌株的潜力进行了测试,如细菌素活性、生物膜形成能力、在脱脂乳中的生长、对抗生素的易感性、对胆汁和低pH值的耐受性以及酶的产生。菌株大多表现出低级别的生物膜形成能力、对抗生素的敏感性和对牛胆/胆汁的足够耐受性。副干酪乳杆菌(Lcb.)ZM-1、ZM-2和乳酸乳球菌(Lc.)PD MO 1/8对pH值3表现出高耐受性(分别为67%、83%和63%)。菌株显示出对主要指示物禽肠球菌EA5的细菌素活性(抑制区范围为5-24 mm)。Lcb的浓缩物质。副酪蛋白LPa ML 12/1(pH 6.3)抑制EA5菌株(抑制活性100 AU ML–1)。Lcb。副酪蛋白ZM-1和ZM-2也产生大量的β-半乳糖苷酶(40nmol)。尽管菌株显示了其有益的潜力,但还需要进行额外的测试;一些测试正在进行中,以进一步可能在乳制品中应用选定的菌株。
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引用次数: 1
Early-Season Effects of Wildfire on Soil Nutrients and Weed Diversity in Two Plantations 季前野火对两种人工林土壤养分和杂草多样性的影响
Q3 Agricultural and Biological Sciences Pub Date : 2021-03-01 DOI: 10.2478/sab-2021-0001
S. Oyedeji, O. O. Agboola, T.S. Oriolowo, D. A. Animasaun, P. Fatoba, A. O. Isichei
Abstract This study assessed early-season effects of spontaneous wildfires on soil fertility and weed diversity in two managed plantations – cashew and teak – in Ilorin, Nigeria. Soil pH and nutrients in the topsoils and subsoils of burned and unburned plantations plots after a spontaneous wildfire were analysed. Species diversity and similarity were determined based on the composition and abundances of weed flora two months post-fire. The fire effects on soil nutrients and weed composition in the plantations were evaluated using the canonical correspondence analysis. Burns incidence significantly improved the organic carbon, organic matter, and Ca contents while reducing total N and Mg in both plantations. Twenty-eight weed species were distributed in 9 angiosperm families. Fire differently affected weed composition, abundance and diversity in the plantations. Burning improved soil organic carbon and organic matter contents and restricted Tephrosia bracteolata, Desmodium tortuosum, Daniellia oliveri, Senna obtusifolia and Zornia latifolia to the burned cashew plantation. The occurrence of Euphorbia heterophylla, Eriosema psoraleoides and Crotalaria retusa in the burned teak plantation was associated with a direct fire effect on soil Na and Ca contents. Burning influenced soil nutrients in the studied plantations, but weed diversity increased in the teak plantation and was reduced in the cashew plantation.
摘要本研究评估了尼日利亚伊洛林两个管理种植园——腰果和柚木——自然野火对土壤肥力和杂草多样性的早季影响。对自然野火后被烧毁和未被烧毁的种植园地块表层土壤和底土的土壤pH值和养分进行了分析。物种多样性和相似性是根据火灾后两个月杂草区系的组成和丰度确定的。采用典型对应分析法评价了火灾对人工林土壤养分和杂草组成的影响。烧伤发生率显著提高了两个人工林的有机碳、有机质和钙含量,同时降低了总氮和总镁。被子植物9科28种。火灾对种植园杂草组成、丰度和多样性的影响各不相同。焚烧提高了土壤有机碳和有机质含量,并限制了小檗、狭叶Desmodium tortuosum、橄榄Daniellia oliveri、钝叶Senna obtusifolia和宽叶Zornia latifolia进入焚烧腰果林。火烧柚木人工林中异叶大戟、补骨脂和马齿苋的发生与火烧对土壤Na和Ca含量的直接影响有关。燃烧影响了所研究种植园的土壤养分,但柚木种植园的杂草多样性增加,腰果种植园的减少。
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引用次数: 0
Lipid Globules and Fatty Acids in Milk of Lactating Raccoon (Nyctereutes procyonoides) 泌乳浣熊乳汁中的脂球和脂肪酸
Q3 Agricultural and Biological Sciences Pub Date : 2021-03-01 DOI: 10.2478/sab-2021-0002
O. Szeleszczuk, P. Kilar, D. Maj
Abstract In this study, milk samples (n=52) from nine healthy female raccoons at 3–45 days of lactation were manually collected at a regular morning hour from all active teats and used to investigate the changes of lipid globules size, fatty acids (FAs) profile and fat content.The results indicated that raccoon milk is characterized by a high fat content. Small lipid globules sizing up to 6 µm prevailed with their greatest share in milk during lactation phases I and III. The milk fat content was increasing with the proceeding lactation, whereas the content of free FAs had a decreasing tendency. Totally eighteen FAs were identified in raccoon milk. The unsaturated long-chain C18–C20 FAs were dominating (over 60%). The individual FAs contents in raccoon milk did not exceed 1%, except for palmitic, vaccenic and linoleic acids representing over 20% of the total FA content.The study results can be used for establishing the energy requirements during the suckling period for proper growth and development of puppies.
摘要在本研究中,在正常的早晨时间,从9只哺乳期3-45天的健康雌性浣熊身上手动采集了所有活动乳头的乳汁样本(n=52),用于研究脂球大小、脂肪酸(FA)分布和脂肪含量的变化。结果表明,貉奶具有脂肪含量高的特点。在哺乳期I和III,尺寸高达6µm的小脂球在乳汁中占主导地位,其份额最大。乳汁脂肪含量随着哺乳期的进行而增加,而游离脂肪酸含量呈下降趋势。在浣熊奶中共鉴定出18个FA。不饱和长链C18–C20脂肪酸占主导地位(超过60%)。除棕榈酸、vaccenic酸和亚油酸占总脂肪酸含量的20%以上外,浣熊奶中的单个脂肪酸含量不超过1%。研究结果可用于确定幼犬在哺乳期的适当生长发育所需的能量。
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引用次数: 0
Fungal Contamination in the Working Environ-Ment of Waste Sorting Facilities: A Review 垃圾分类设施工作环境中的真菌污染研究进展
Q3 Agricultural and Biological Sciences Pub Date : 2020-12-01 DOI: 10.2478/sab-2020-0013
K. Perná, Z. Wittlingerová, M. Zimová
Abstract In recent years, many studies have pointed to a relationship between the working activity in waste sorting facilities and the occurrence of health problems (respiratory, gastrointestinal, musculoskeletal, skin and eye irritations). Employees in these facilities are simultaneously exposed to many biological, chemical and physical factors. Due to the repeated detection of high concentrations of airborne fungi (reaching up to 1.8 x 106 CFU m–3) in the working environment involving potentially allergenic, infectious and toxigenic fungal species, it can be assumed that fungi may play an important role in the development of health problems. In terms of minimizing health risks, it is necessary to take several preventive and protective measures to reduce contamination of the working environment by biological agents. The basic recommendation is the consistent use of protective equipment as well as the observance of personal hygiene by the employees. Other necessary measures are the frequent air exchange inside the facility, educating the employees about health risks associated with waste sorting and establishment of regular medical check-ups.
近年来,许多研究指出垃圾分类设施的工作活动与健康问题(呼吸、胃肠道、肌肉骨骼、皮肤和眼睛刺激)的发生之间存在关系。这些设施的员工同时暴露在许多生物、化学和物理因素中。由于在工作环境中反复检测到高浓度的空气传播真菌(高达1.8 x 106 CFU m-3),涉及潜在的致敏性,感染性和产毒性真菌物种,可以假设真菌可能在健康问题的发展中发挥重要作用。在尽量减少健康风险方面,有必要采取若干预防和保护措施,以减少生物制剂对工作环境的污染。基本建议是员工始终使用防护设备并遵守个人卫生。其他必要措施包括设施内经常换气,教育员工了解与废物分类有关的健康风险,并建立定期体检。
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引用次数: 1
Costs and Benefits of Green Tramway Tracks 绿色电车轨道的成本和效益
Q3 Agricultural and Biological Sciences Pub Date : 2020-12-01 DOI: 10.2478/sab-2020-0012
E. Jakubcová, E. Horváthová
Abstract In recent decades, green tramway tracks have still more often been installed in many cities as an alternative to standard rails placed on concrete sleepers or grooved rails since the vegetated tracks are beneficial to urban dwellers. In this article, we summarize and compare the benefits of grass and low-maintenance tramway tracks and link them with their investment and maintenance costs in two Czech cities. We conclude that grass surfaces offer a slightly higher rainwater retention capacity, while the rest of the benefits are similar for grass and low-maintenance surfaces. The investment costs are also similar, however, the maintenance costs are 30× higher for grass surfaces than for the low-maintenance ones.
近几十年来,由于绿化轨道对城市居民有利,许多城市仍然经常安装绿色有轨电车轨道,以替代混凝土轨枕或沟槽轨道上的标准轨道。在这篇文章中,我们总结和比较了捷克两个城市的草地和低维护电车轨道的好处,并将它们与投资和维护成本联系起来。我们得出的结论是,草表面提供了稍高的雨水保留能力,而其他的好处与草和低维护表面相似。投资成本也相似,但草坪表面的维护成本比低维护的草坪表面高30倍。
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引用次数: 1
Integration of Spectral Measurement and UAV for Paddy Leaves Chlorophyll Content Estimation 光谱测量与无人机集成用于水稻叶片叶绿素含量估算
Q3 Agricultural and Biological Sciences Pub Date : 2020-09-01 DOI: 10.2478/sab-2020-0011
M. F. Ghazali, K. Wikantika, I. Aryantha, R. Maulani, L. F. Yayusman, D. Sumantri
Abstract In the agriculture sector, proper crop management can enhance yield production. Determination of the chlorophyll content in crop contributes to this significant topic. In this study, the leaves chlorophyll content of local paddy cultivars Inpari 32 and Inpari 33 was estimated and the difference at various days after planting (DAP) was determined. The procedure involved the combination of spectral reflectance data, aerial photographs taken by unmanned aerial vehicle (UAV), and chlorophyll laboratory analysis data. The chlorophyll content in the cultivars leaves was estimated using the models MCARIspectroradiometer and UAV chlorophyll regression (UCR). The results showed a variation in the chlorophyll content not only between the two cultivars on various DAP, but randomly also in the same cultivar of the same DAP. The MCARIspectroradiometer model indicated a lower chlorophyll content for Inpari 32 than for Inpari 33 while the UCR model gave opposite results. The chlorophyll content raises with increasing DAP, but it gradually decreases through the grain filling period until harvest.
摘要在农业部门,适当的作物管理可以提高产量。作物中叶绿素含量的测定有助于这一重要课题的研究。在本研究中,估计了当地水稻品种Inpari 32和Inpari 33的叶片叶绿素含量,并测定了种植后不同天数的差异(DAP)。该程序涉及光谱反射率数据、无人机拍摄的航空照片和叶绿素实验室分析数据的组合。利用MCARI光谱辐射计和UAV叶绿素回归(UCR)模型对品种叶片中的叶绿素含量进行了估算。结果表明,叶绿素含量不仅在不同DAP的两个品种之间存在差异,而且在相同DAP的同一品种中也存在随机变化。MCARI光谱辐射计模型显示Inpari 32的叶绿素含量低于Inpari 33,而UCR模型给出了相反的结果。叶绿素含量随DAP的增加而增加,但在灌浆期至收获期逐渐降低。
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引用次数: 4
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Scientia Agriculturae Bohemica
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