R. Sigalingging, Sumono, D. Herák, A. Kabutey, C. Sigalingging
Abstract A mathematical model for effective porosity, effective force and permeability was derived using the experimental results. A universal compression machine was used to press oil of jatropha seeds at maximum force 100 kN, pressing vessel diameter 60 mm, initial pressing height 60 mm with different compression speeds ranging from 1 to 50 mm.min−1. The analysis of variance showed significant effects of compression speed on oil yield, oil point deformation, effective porosity and time of oil flow (P < 0.05), while the compression speed effect was not significant on the maximum deformation, oil point force, effective force, flow rate of oil and permeability (P > 0.05). Oil yield decreased significantly with increasing speed hence lower driving effective force. In addition, lower effective porosity required higher effective force to drive oil flow due to lower permeability and flow rate of jatropha seeds oil. Equilibrium force between effective force and oil point force at force 100 kN was determined to be 50 kN with corresponding compression speed approximately 42.74 mm.min−1. This knowledge is important in the industrial technology of oil processing where higher pressure is needed to achieve maximum leakage of oil.
{"title":"Influence of Porosity, Permeability and Expression Force on Oil Yield of Jatropha Seeds","authors":"R. Sigalingging, Sumono, D. Herák, A. Kabutey, C. Sigalingging","doi":"10.2478/sab-2019-0009","DOIUrl":"https://doi.org/10.2478/sab-2019-0009","url":null,"abstract":"Abstract A mathematical model for effective porosity, effective force and permeability was derived using the experimental results. A universal compression machine was used to press oil of jatropha seeds at maximum force 100 kN, pressing vessel diameter 60 mm, initial pressing height 60 mm with different compression speeds ranging from 1 to 50 mm.min−1. The analysis of variance showed significant effects of compression speed on oil yield, oil point deformation, effective porosity and time of oil flow (P < 0.05), while the compression speed effect was not significant on the maximum deformation, oil point force, effective force, flow rate of oil and permeability (P > 0.05). Oil yield decreased significantly with increasing speed hence lower driving effective force. In addition, lower effective porosity required higher effective force to drive oil flow due to lower permeability and flow rate of jatropha seeds oil. Equilibrium force between effective force and oil point force at force 100 kN was determined to be 50 kN with corresponding compression speed approximately 42.74 mm.min−1. This knowledge is important in the industrial technology of oil processing where higher pressure is needed to achieve maximum leakage of oil.","PeriodicalId":53537,"journal":{"name":"Scientia Agriculturae Bohemica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46540490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract The study identifies the main beer consumption determinants in the Czech Republic and quantifies their influence on the beer consumption. The analysis covers three areas – identification of the main determinants of Czech beer consumption, quantification of the influence of these factors on beer consumption, and examination of beer consumption forecasting possibilities. Time series of selected variables containing annual data of the 1994–2014 period are analysed. The main determinants are defined in accord with the general economic theory, their influence is measured using an econometric model, and quality of the model for prognostic purposes is verified using an ex-post prognosis. Based on empirical analysis, the price of lager beer, income, and influence of financial crisis were determined as the main determinants of Czech beer consumption. In addition, inelastic reaction of beer consumption on their changes was proven. The estimated model may be considered suitable for prognostic purposes. The Czech beer market and/or beer consumption in the examined period can be considered as specific, but stable.
{"title":"Beer Consumption in the Czech Republic and its Determinants","authors":"L. Rumankova, P. Sanova, P. Kolář","doi":"10.2478/sab-2019-0010","DOIUrl":"https://doi.org/10.2478/sab-2019-0010","url":null,"abstract":"Abstract The study identifies the main beer consumption determinants in the Czech Republic and quantifies their influence on the beer consumption. The analysis covers three areas – identification of the main determinants of Czech beer consumption, quantification of the influence of these factors on beer consumption, and examination of beer consumption forecasting possibilities. Time series of selected variables containing annual data of the 1994–2014 period are analysed. The main determinants are defined in accord with the general economic theory, their influence is measured using an econometric model, and quality of the model for prognostic purposes is verified using an ex-post prognosis. Based on empirical analysis, the price of lager beer, income, and influence of financial crisis were determined as the main determinants of Czech beer consumption. In addition, inelastic reaction of beer consumption on their changes was proven. The estimated model may be considered suitable for prognostic purposes. The Czech beer market and/or beer consumption in the examined period can be considered as specific, but stable.","PeriodicalId":53537,"journal":{"name":"Scientia Agriculturae Bohemica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41418117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Antibiotic resistance represents a serious threat worldwide. When considering the increasing ability of bacteria to effectively resist antibacterial agents, it is necessary to reduce the consumption of antibiotic substances in animal production in order to preserve their effectiveness in the future. Attention should be paid to the multidrug resistant microorganisms’ occurrence, which can be very exhausting for the breeder not only from the economic point of view. Therefore, alternative sources of antibacterials should be considered due to the limited possibilities of using conventional antibiotics in animal breeding, e.g. application of various substances including organic acids, clay minerals, etc. Nowadays, the research in this field also focuses on the combinatory effect of such compounds, which can also find the perspective for use in animal breeding. This article provides an overview of problems connected with the resistance of diverse bacteria to antibiotic treatment in livestock breeding. It emphasises the need for alternate resources usage with the aim to lower the environmental burden caused by overuse of antimicrobials used in subclinical doses in the past and with the expanding bacterial resistance.
{"title":"Antibiotic Resistance in Livestock Breeding: A Review","authors":"K. Lalouckova, E. Skřivanová","doi":"10.2478/sab-2019-0003","DOIUrl":"https://doi.org/10.2478/sab-2019-0003","url":null,"abstract":"Abstract Antibiotic resistance represents a serious threat worldwide. When considering the increasing ability of bacteria to effectively resist antibacterial agents, it is necessary to reduce the consumption of antibiotic substances in animal production in order to preserve their effectiveness in the future. Attention should be paid to the multidrug resistant microorganisms’ occurrence, which can be very exhausting for the breeder not only from the economic point of view. Therefore, alternative sources of antibacterials should be considered due to the limited possibilities of using conventional antibiotics in animal breeding, e.g. application of various substances including organic acids, clay minerals, etc. Nowadays, the research in this field also focuses on the combinatory effect of such compounds, which can also find the perspective for use in animal breeding. This article provides an overview of problems connected with the resistance of diverse bacteria to antibiotic treatment in livestock breeding. It emphasises the need for alternate resources usage with the aim to lower the environmental burden caused by overuse of antimicrobials used in subclinical doses in the past and with the expanding bacterial resistance.","PeriodicalId":53537,"journal":{"name":"Scientia Agriculturae Bohemica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42864684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Nematodes of the genus Setaria (Spirurida, Filarioidea) are parasites of vertebrates except fish. Dangerous are their larvae (microfilariae) that may migrate to the central nervous system of hosts causing serious neuropathic disorders of vertebrates including human. Setaria cervi, S. labiatopapillosa, and S. tundra are potential parasites in wild ruminants in Europe. The most recent studies of variability among Setaria spp. are based on a combination of morphometric and molecular methods. Although there is no clear consistency in the morphological structures used by different authors, a morphological key was compiled based on the body length, the arrangement of peribucal crown, and the number of papillae in the caudal part of the body. The barcoding system of Setaria genus is based on sequences of conserved gene polymorphisms such as COX1 and rDNA genes. The published sequences of COX1 gene in Setaria populations were analysed using in silico phylogenetic analysis by the maximum likelihood method (Tamura-Nei model). This analysis confirmed that the COX1 nucleotide polymorphisms genes are species-specific and represent the theoretical basis for the development of markers enabling barcoding system in the genus Setaria.
{"title":"Interspecific Variability of Filarioid Nematodes of the Genus Setaria Viborg, 1795 Occurring in Wild Ruminants in Europe: A Review","authors":"S. Lanková, P. Vejl, M. Melounová, I. Langrová","doi":"10.2478/sab-2019-0005","DOIUrl":"https://doi.org/10.2478/sab-2019-0005","url":null,"abstract":"Abstract Nematodes of the genus Setaria (Spirurida, Filarioidea) are parasites of vertebrates except fish. Dangerous are their larvae (microfilariae) that may migrate to the central nervous system of hosts causing serious neuropathic disorders of vertebrates including human. Setaria cervi, S. labiatopapillosa, and S. tundra are potential parasites in wild ruminants in Europe. The most recent studies of variability among Setaria spp. are based on a combination of morphometric and molecular methods. Although there is no clear consistency in the morphological structures used by different authors, a morphological key was compiled based on the body length, the arrangement of peribucal crown, and the number of papillae in the caudal part of the body. The barcoding system of Setaria genus is based on sequences of conserved gene polymorphisms such as COX1 and rDNA genes. The published sequences of COX1 gene in Setaria populations were analysed using in silico phylogenetic analysis by the maximum likelihood method (Tamura-Nei model). This analysis confirmed that the COX1 nucleotide polymorphisms genes are species-specific and represent the theoretical basis for the development of markers enabling barcoding system in the genus Setaria.","PeriodicalId":53537,"journal":{"name":"Scientia Agriculturae Bohemica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45448960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract The prevalence and intensity of infection of the lungworm Muellerius capillaris in dairy and young goats were evaluated at one organic farm in the Czech Republic. A total of 605 faecal samples were collected from rectum of thirty selected dairy and thirty young goats; each individual was examined monthly throughout a year. Data were statistically evaluated to verify the existence of differences in values of larvae count per gram (LPG) in dairy and young goats. Further, dairy goats were divided into three groups depending on the number of lactations and the differences in LPG values between groups were statistically evaluated. The species M. capillaris was the only lung parasite identified during our study with an overall prevalence 87.2 % and 93.1 % for young goats and dairy goats, respectively. The difference in the larvae count between young and dairy goats was not statistically significant. The comparison of larvae counts in goat groups depending on the number of lactation showed significant differences between dairy goats on the first and second lactation and between goats on the second and third lactation (P < 0.05) contrary to larvae count between goats on the first and third lactation.
{"title":"Comparison of Lungworm Infection in a Herd of Young and Dairy Goats at an Organic Farm","authors":"I. A. Kyriánová, J. Vadlejch, I. Langrová","doi":"10.2478/sab-2019-0004","DOIUrl":"https://doi.org/10.2478/sab-2019-0004","url":null,"abstract":"Abstract The prevalence and intensity of infection of the lungworm Muellerius capillaris in dairy and young goats were evaluated at one organic farm in the Czech Republic. A total of 605 faecal samples were collected from rectum of thirty selected dairy and thirty young goats; each individual was examined monthly throughout a year. Data were statistically evaluated to verify the existence of differences in values of larvae count per gram (LPG) in dairy and young goats. Further, dairy goats were divided into three groups depending on the number of lactations and the differences in LPG values between groups were statistically evaluated. The species M. capillaris was the only lung parasite identified during our study with an overall prevalence 87.2 % and 93.1 % for young goats and dairy goats, respectively. The difference in the larvae count between young and dairy goats was not statistically significant. The comparison of larvae counts in goat groups depending on the number of lactation showed significant differences between dairy goats on the first and second lactation and between goats on the second and third lactation (P < 0.05) contrary to larvae count between goats on the first and third lactation.","PeriodicalId":53537,"journal":{"name":"Scientia Agriculturae Bohemica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44005724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract The effect of endogenous estrogens on the male reproductive tissues and development of gametes is an essential for the reproductive success. Estrogens affect the target cells via estrogen receptors (ERs) by both genomic and non-genomic pathways. The ER localization in the testis, epididymis, and sperm cells is a key to understanding the effect of estrogens on the sperm development, maturation, and function. The ER detection in male reproductive tissues and sperm cells at different development stages is described in representative mammalian species (human, mouse, rat, horse, and pig), in which the ER localization has been most described. According to various authors the ER occurrence in the male reproductive tissues and spermatozoa is quite distinct. Discrepancy in the published results is probably caused either by the application of different tissue preparation methods, or the choice of specific antibodies. Inconsistent findings should be subjected to further investigation to better understand the role of ERs in the male gamete development and mammalian reproduction
{"title":"Localization of Estrogen Receptors in Male Reproductive Tissues and Sperm Cells – A Review","authors":"R. Krejčířová, P. Postlerová, R. Rajmon","doi":"10.2478/sab-2018-0034","DOIUrl":"https://doi.org/10.2478/sab-2018-0034","url":null,"abstract":"Abstract The effect of endogenous estrogens on the male reproductive tissues and development of gametes is an essential for the reproductive success. Estrogens affect the target cells via estrogen receptors (ERs) by both genomic and non-genomic pathways. The ER localization in the testis, epididymis, and sperm cells is a key to understanding the effect of estrogens on the sperm development, maturation, and function. The ER detection in male reproductive tissues and sperm cells at different development stages is described in representative mammalian species (human, mouse, rat, horse, and pig), in which the ER localization has been most described. According to various authors the ER occurrence in the male reproductive tissues and spermatozoa is quite distinct. Discrepancy in the published results is probably caused either by the application of different tissue preparation methods, or the choice of specific antibodies. Inconsistent findings should be subjected to further investigation to better understand the role of ERs in the male gamete development and mammalian reproduction","PeriodicalId":53537,"journal":{"name":"Scientia Agriculturae Bohemica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41339233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Benefits of open data are diverse and range from improved efficiency of public administrations, economic growth in the private sector. Agriculture is also an inseparable part of the private sector. These data can stimulate economic growth. Economy can benefit from an easier access to information, content and knowledge in turn contributing to the development of innovative services and creation of new business models (European Data Portal, 2016). The prerequisite of this stimulation is consistency of open data. Both of these features have to be a part of these data quality. However, this aspect of open data quality has not yet been satisfactorily resolved in the framework of international standardization of quality (Systems and Software Quality Requirements and Evaluation (SQuaRE)). The issue of possible evaluation of open data consistency in agriculture is discussed. Results suggest that open data consistency may be achieved by consistent application of the technique of data normalization of relevant data sets. Consistent application of data normalization technique of open data sets can reduce the risk of inconsistency of the open data. That is the only way to guarantee that the open data will be the benefit to private sector.
{"title":"Consistency of Open Data as Prerequisite for Usability in Agriculture","authors":"V. Vostrovský, J. Tyrychtr","doi":"10.2478/sab-2018-0040","DOIUrl":"https://doi.org/10.2478/sab-2018-0040","url":null,"abstract":"Abstract Benefits of open data are diverse and range from improved efficiency of public administrations, economic growth in the private sector. Agriculture is also an inseparable part of the private sector. These data can stimulate economic growth. Economy can benefit from an easier access to information, content and knowledge in turn contributing to the development of innovative services and creation of new business models (European Data Portal, 2016). The prerequisite of this stimulation is consistency of open data. Both of these features have to be a part of these data quality. However, this aspect of open data quality has not yet been satisfactorily resolved in the framework of international standardization of quality (Systems and Software Quality Requirements and Evaluation (SQuaRE)). The issue of possible evaluation of open data consistency in agriculture is discussed. Results suggest that open data consistency may be achieved by consistent application of the technique of data normalization of relevant data sets. Consistent application of data normalization technique of open data sets can reduce the risk of inconsistency of the open data. That is the only way to guarantee that the open data will be the benefit to private sector.","PeriodicalId":53537,"journal":{"name":"Scientia Agriculturae Bohemica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46728338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. Jarosova, I. Doskočil, T. Volstatova, J. Havlík
Abstract The ability of bacteria to adhere to the intestinal epithelial cells is one of the main criteria for selection of new probiotic strains. Some dietary polyphenols have been proven to affect bacterial adhesion, providing a rationale for the use of mixtures of polyphenols and probiotics. Resveratrol, a naturally occurring stilbene in plants, has been shown to have a number of beneficial biological effects. The adhesion ability of four Lactobacillus strains (Lactobacillus brevis, L. fermentum, L. gasseri, and L. plantarum), in the presence of resveratrol, has been investigated in an in vitro model based on mixed co-culture of Caco-2 and HT29-MTX intestinal epithelial cells. The effective concentration of resveratrol used in the adhesion experiment has been selected based on cytotoxicity test. Resveratrol at three physiologically low concentrations (4.5, 2.25, and 1.125 µg ml−1), added together with the bacterial suspension, had no statistically significant influence on the adhesion of any strain (P < 0.05). Since the health benefits of polyphenols are often associated with the composition of gut microbiota, the knowledge of interactions between known bacteria and polyphenols would be of high scientific value.
{"title":"Adhesive Property of Different Strains of Lactobacilli in The Presence of Resveratrol","authors":"V. Jarosova, I. Doskočil, T. Volstatova, J. Havlík","doi":"10.2478/sab-2018-0036","DOIUrl":"https://doi.org/10.2478/sab-2018-0036","url":null,"abstract":"Abstract The ability of bacteria to adhere to the intestinal epithelial cells is one of the main criteria for selection of new probiotic strains. Some dietary polyphenols have been proven to affect bacterial adhesion, providing a rationale for the use of mixtures of polyphenols and probiotics. Resveratrol, a naturally occurring stilbene in plants, has been shown to have a number of beneficial biological effects. The adhesion ability of four Lactobacillus strains (Lactobacillus brevis, L. fermentum, L. gasseri, and L. plantarum), in the presence of resveratrol, has been investigated in an in vitro model based on mixed co-culture of Caco-2 and HT29-MTX intestinal epithelial cells. The effective concentration of resveratrol used in the adhesion experiment has been selected based on cytotoxicity test. Resveratrol at three physiologically low concentrations (4.5, 2.25, and 1.125 µg ml−1), added together with the bacterial suspension, had no statistically significant influence on the adhesion of any strain (P < 0.05). Since the health benefits of polyphenols are often associated with the composition of gut microbiota, the knowledge of interactions between known bacteria and polyphenols would be of high scientific value.","PeriodicalId":53537,"journal":{"name":"Scientia Agriculturae Bohemica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44139771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Rizaldi, W. Hermawan, T. Mandang, S. Pertiwi, Rudiyanto
Abstract During operation, the lug wheels penetrate the soil and form certain angle to the soil surface at varying penetration depths. In order to determine the soil reaction force against plate penetration given to the soil, penetrometer was mounted on the plate. Plate sizes used in this experiment were 5×5 cm2, 5×10 cm2, 5×15 cm2 and 5×20 cm2. The measurement was carried out at varying angles and depths i.e. 90°, 75°, 60°, 45°, 30° and 4 cm, 8 cm, 12 cm and 16 cm, respectively. The objective of this research was to develop the method on the prediction of soil plat penetration resistance at varying depths and angles. Prediction method was developed using linear or polynomial regression method which compared with Artificial Neural Network (ANN). The research showed that ANN generated better prediction value which indicated by lower error magnitude compared with regression method i.e. 9.9% and 19.7%, respectively.
摘要在操作过程中，车轮会穿透土壤，并在不同的穿透深度下与土壤表面形成一定的角度。为了确定土壤对板渗透的反作用力，在板上安装了贯入仪。本实验中使用的板尺寸分别为5×5 cm2、5×10 cm2、5 x 15 cm2和5×20 cm2。测量在不同的角度和深度进行，即分别为90°、75°、60°、45°、30°和4cm、8cm、12cm和16cm。本研究的目的是开发在不同深度和角度下预测土壤平台渗透阻力的方法。将线性或多项式回归方法与人工神经网络（ANN）进行了比较，提出了预测方法。研究表明，与回归方法相比，人工神经网络产生了更好的预测值，误差幅度较小，分别为9.9%和19.7%。
{"title":"Development of The Method on The Prediction of Soil Plat Penetration Resistance","authors":"T. Rizaldi, W. Hermawan, T. Mandang, S. Pertiwi, Rudiyanto","doi":"10.2478/sab-2018-0039","DOIUrl":"https://doi.org/10.2478/sab-2018-0039","url":null,"abstract":"Abstract During operation, the lug wheels penetrate the soil and form certain angle to the soil surface at varying penetration depths. In order to determine the soil reaction force against plate penetration given to the soil, penetrometer was mounted on the plate. Plate sizes used in this experiment were 5×5 cm2, 5×10 cm2, 5×15 cm2 and 5×20 cm2. The measurement was carried out at varying angles and depths i.e. 90°, 75°, 60°, 45°, 30° and 4 cm, 8 cm, 12 cm and 16 cm, respectively. The objective of this research was to develop the method on the prediction of soil plat penetration resistance at varying depths and angles. Prediction method was developed using linear or polynomial regression method which compared with Artificial Neural Network (ANN). The research showed that ANN generated better prediction value which indicated by lower error magnitude compared with regression method i.e. 9.9% and 19.7%, respectively.","PeriodicalId":53537,"journal":{"name":"Scientia Agriculturae Bohemica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49266067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Malignant melanoma may be a life-threatening disease caused by various conditions. Cutaneous bacteria could play a role in melanoma development or regression. The present work aimed to analyze the bacterial species present on the epidermis of piglets with hereditary melanoma. Bacteria isolated by swabs directly from melanomas and healthy epidermis were analysed using MALDI-TOF mass spectrometry. From the total of 290 isolates, 92 were identified, while the extraction by ethanol turned out to be more efficient compared to identification by direct transfer. Staphylococcus sciuri, Staphylococcus cohnii, and Lactococcus lactis were significantly more frequent on healthy skin, whereas Staphylococcus chromogenes, Staphylococcus hyicus, and Enterococcus faecalis have thrived significantly better on melanoma. Overall, the results indicate that the micro-biota of melanoma is different from that of healthy epidermis, so piglet skin bacteria inspections are recommended.
{"title":"Analysis of Cutaneous Microbiota of Piglets with Hereditary Melanoma","authors":"R. Svejstil, H. Salmonova, J. Čížková","doi":"10.2478/sab-2018-0035","DOIUrl":"https://doi.org/10.2478/sab-2018-0035","url":null,"abstract":"Abstract Malignant melanoma may be a life-threatening disease caused by various conditions. Cutaneous bacteria could play a role in melanoma development or regression. The present work aimed to analyze the bacterial species present on the epidermis of piglets with hereditary melanoma. Bacteria isolated by swabs directly from melanomas and healthy epidermis were analysed using MALDI-TOF mass spectrometry. From the total of 290 isolates, 92 were identified, while the extraction by ethanol turned out to be more efficient compared to identification by direct transfer. Staphylococcus sciuri, Staphylococcus cohnii, and Lactococcus lactis were significantly more frequent on healthy skin, whereas Staphylococcus chromogenes, Staphylococcus hyicus, and Enterococcus faecalis have thrived significantly better on melanoma. Overall, the results indicate that the micro-biota of melanoma is different from that of healthy epidermis, so piglet skin bacteria inspections are recommended.","PeriodicalId":53537,"journal":{"name":"Scientia Agriculturae Bohemica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42443080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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