Progress in Biophysics & Molecular Biology最新文献

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The application progress of cell membrane biomimetic nanoparticles in cancer diagnosis and treatment1 细胞膜仿生纳米颗粒在肿瘤诊断与治疗中的应用进展

IF 3.2 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Progress in Biophysics & Molecular Biology

Pub Date : 2025-06-02 DOI: 10.1016/j.pbiomolbio.2025.05.004

Baiyan Wang , Jiayi Li , Dandan Guo , Shuxuan Li , Yalan Li , Qianqian Wang , Yi Yang , Wei Chen , Shuying Feng

Cancer diagnosis and treatment remains challenging, with unresolved issues such as low targeting, drug resistance, and numerous adverse reactions from chemotherapy. Cell membrane biomimetic modified nanoparticles(CMBMNPs), wrapping cell membrane on nanoparticles can achieve homologous cell mimicry, so that it can obtain the functions and properties of the type of cell, with strong targeting ability, strong immune evasion ability, long in vivo circulation time, etc., which is getting more and more attention. This article describes the preparation process of CMBMNPs and the different clinical effects of different types of nanoparticles and mimicking cell membranes in order to select the right match for use. In addition, we list in detail several important features of CMBMNPs as well as the advantages of CMBMNPs in areas related to cancer diagnosis and therapy, and look forward to the future challenges and prospects of cell membrane-generating nanotechnology, which provides new insights into the application of CMBMNPs in cancer diagnosis and therapy.

癌症的诊断和治疗仍然具有挑战性，如低靶向性、耐药性和化疗的众多不良反应等尚未解决的问题。细胞膜仿生修饰纳米颗粒（CMBMNPs），在纳米颗粒上包裹细胞膜可以实现同源细胞的模仿，使其能够获得细胞类型的功能和特性，具有靶向能力强、免疫逃避能力强、体内循环时间长等特点，越来越受到人们的关注。本文介绍了CMBMNPs的制备过程，以及不同类型的纳米颗粒和模拟细胞膜的不同临床效果，以便选择合适的配型使用。此外，我们详细列出了CMBMNPs的几个重要特性以及CMBMNPs在癌症诊断和治疗相关领域的优势，并展望了未来膜生成纳米技术的挑战和前景，为CMBMNPs在癌症诊断和治疗中的应用提供了新的见解。

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引用次数: 0

Lipid droplet - organelle crosstalk and its implication in cancer 脂滴-细胞器串扰及其在癌症中的意义。

IF 3.2 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Progress in Biophysics & Molecular Biology

Pub Date : 2025-05-15 DOI: 10.1016/j.pbiomolbio.2025.05.002

Jing Quan , Chunhong Zhang , Xue Chen , Xinfei Cai , Xiangjian Luo

Lipid droplets (LDs) store lipids in cells, provide phospholipids for membrane synthesis, and maintain the intracellular balance of energy and lipid metabolism. Undoubtedly, the crosstalk between LDs and other organelles is the foundation for performing functions. Many studies indicate that LDs promote tumor progression. LD accumulation has been observed in a variety of cancers, and high LD content is associated with malignant phenotype and poor prognosis of cancers. In this paper, we summarized the intimate crosstalk between LDs and intracellular organelles, including endoplasmic reticulum (ER), mitochondria, lysosomes and peroxisomes, and addressed the effects of LD-organelle crosstalk on cancer initiation and progression. We also integrated the changes of LD-organelle interactions in cancers to provide an insightful knowledge for cancer therapeutics.

脂滴在细胞内储存脂质，为细胞膜合成提供磷脂，维持细胞内能量和脂质代谢的平衡。毫无疑问，lcd与其他细胞器之间的串扰是实现功能的基础。许多研究表明ld促进肿瘤进展。多种癌症均存在LD积累现象，LD含量高与癌症的恶性表型和预后不良有关。本文综述了ldds与细胞内细胞器（包括内质网、线粒体、溶酶体和过氧化物酶体）之间的密切串扰，并探讨了ldds与细胞器之间的串扰对癌症发生和发展的影响。我们还整合了ld -细胞器相互作用在癌症中的变化，为癌症治疗提供了有见地的知识。

引用次数: 0

Advances in the mechanisms of HIF-1α-enhanced tumor glycolysis and its relation to dedifferentiation hif -1α-增强肿瘤糖酵解机制及其与去分化关系的研究进展。

IF 3.2 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Progress in Biophysics & Molecular Biology

Pub Date : 2025-05-13 DOI: 10.1016/j.pbiomolbio.2025.05.003

Yu Zeng , Yonggang Tao , Guotu Du , Tianyu Huang , Shicheng Chen , Longmei Fan , Neng Zhang

Metabolic reprogramming, a hallmark of malignancy, enables tumor cells to adapt to the harsh and dynamic tumor microenvironment (TME) by altering metabolic pathways. Hypoxia, prevalent in solid tumors, activates hypoxia inducible factor 1α (HIF-1α). HIF-1α drives metabolic reprogramming, enhancing glycolysis primarily through the Warburg effect to reduce oxygen dependence and facilitate tumor cell growth/proliferation. The above process is associated with accelerated tumor cell dedifferentiation and enhanced stemness, generating cancer stem cells (CSCs) which possesses the potential for self-renewal and differentiation that can differentiate into a wide range of subtypes of tumor cells and fuel tumor heterogeneity, metastasis, and recurrence, complicating therapy. This review examines the HIF-1α-glycolysis-dedifferentiation crosstalk mechanisms, expecting that indirect inhibition of HIF-1α by targeting metabolic enzymes, metabolites, or their signaling pathways will offer an effective therapeutic strategy to improve the cancer treatment outcomes.

代谢重编程是恶性肿瘤的一个标志，它通过改变代谢途径使肿瘤细胞适应恶劣和动态的肿瘤微环境（TME）。缺氧在实体肿瘤中普遍存在，可激活缺氧诱导因子1α (HIF-1α)。HIF-1α驱动代谢重编程，主要通过Warburg效应增强糖酵解，从而降低氧依赖性，促进肿瘤细胞生长/增殖。上述过程与肿瘤细胞去分化加速和干性增强有关，产生具有自我更新和分化潜力的癌症干细胞（cancer stem cells, CSCs），可以分化成广泛的肿瘤细胞亚型，并促进肿瘤异质性、转移和复发，使治疗复杂化。本文综述了HIF-1α-糖酵解-去分化串串机制，期望通过靶向代谢酶、代谢物或其信号通路间接抑制HIF-1α将为改善癌症治疗效果提供有效的治疗策略。

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引用次数: 0

The key role of Piezo1 channels in ferroptosis after spinal cord injury and the therapeutic potential of Piezo1 inhibitors Piezo1通道在脊髓损伤后铁下垂中的关键作用及Piezo1抑制剂的治疗潜力

IF 3.2 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Progress in Biophysics & Molecular Biology

Pub Date : 2025-05-06 DOI: 10.1016/j.pbiomolbio.2025.05.001

Qianxi Li, Chenyu Li, Xinyu Liu, Zixuan Guo, Xinxin Li, Xin Zhang

Background

Ferroptosis has been confirmed to be one of the key mechanisms of neuronal injury and dysfunction after spinal cord injury (SCI). Mechanical stresses such as deformation, compression, and stretching not only directly cause physical damage to spinal cord tissue at the moment of SCI, but also promote the development of ferroptosis through various pathways. However, the mechanism of ferroptosis after SCI remains unclear, which hinders the development of therapeutic methods.

Objective

This article aims to review the key mechanisms by which mechanical stress affects ferroptosis after SCI, including its impact on the structure and function of the endoplasmic reticulum (ER) and mitochondria, its role in triggering inflammatory responses, and its activation of mechanosensitive channels. Special emphasis is placed on the role of Piezo1 channels, which are key factors in cell mechanosensation and ion homeostasis regulation. The review explores how Piezo1 channels are upregulated by mechanical stress after SCI and participate in the ferroptosis process by mediating ion flow and other mechanisms.

Conclusions

Inhibiting Piezo1 channels may be a potential therapeutic strategy for SCI. This review summarizes the therapeutic potential of Piezo1 inhibitors by sorting out existing studies, hoping to provide a theoretical basis for effective therapeutic strategies targeting ferroptosis after SCI.

背景：铁下垂已被证实是脊髓损伤（SCI）后神经元损伤和功能障碍的重要机制之一。变形、压缩、拉伸等机械应力不仅直接造成脊髓损伤瞬间脊髓组织的物理损伤，而且通过多种途径促进铁下垂的发生发展。然而，脊髓损伤后铁下垂的机制尚不清楚，这阻碍了治疗方法的发展。目的综述机械应力影响脊髓损伤后铁吊的主要机制，包括机械应力对内质网和线粒体结构和功能的影响、机械应力在引发炎症反应中的作用以及机械敏感通道的激活。特别强调的是Piezo1通道的作用，这是细胞机械感觉和离子稳态调节的关键因素。本文探讨了脊髓损伤后机械应力如何上调Piezo1通道，并通过介导离子流动等机制参与铁凋亡过程。结论抑制Piezo1通道可能是一种治疗脊髓损伤的潜在策略。本文通过对已有研究的梳理，总结了Piezo1抑制剂的治疗潜力，希望为制定针对脊髓损伤后铁下垂的有效治疗策略提供理论依据。

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引用次数: 0

From gene editing to tumor eradication: The CRISPR revolution in cancer therapy 从基因编辑到肿瘤根除：癌症治疗中的CRISPR革命

IF 3.2 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Progress in Biophysics & Molecular Biology

Pub Date : 2025-04-16 DOI: 10.1016/j.pbiomolbio.2025.04.003

Ashiq Ali , Urooj Azmat , Aisha Khatoon , Kaynaat Akbar , Bilal Murtaza , Ziyi Ji , Urooj Irshad , Zhongjing Su

Cancer continues to be a significant worldwide health concern, characterized by high rates of occurrence and death. Unfortunately, existing treatments frequently fall short of delivering satisfying therapeutic outcomes. Immunotherapy has ushered in a new era in the treatment of solid tumors, yet its effectiveness is still constrained and comes with unwanted side effects. The advancement of cutting-edge technology, propelled by gene analysis and manipulation at the molecular scale, shows potential for enhancing these therapies. The advent of genome editing technologies, including CRISPR-Cas9, can greatly augment the efficacy of cancer immunotherapy. This review explores the mechanism of CRISPR-Cas9-mediated genome editing and its wide range of tools. The study focuses on analyzing the effects of CRISPR-induced double-strand breaks (DSBs) on cancer immunotherapy, specifically by gene knockdown or knockin. In addition, the study emphasizes the utilization of CRISPR-Cas9-based genome-wide screening to identify targets, the potential of spatial CRISPR genomics, and the extensive applications and difficulties of CRISPR-Cas9 in fundamental research, translational medicine, and clinical environments.

癌症仍然是世界范围内一个重大的健康问题，其特点是发病率和死亡率都很高。不幸的是，现有的治疗方法往往不能提供令人满意的治疗结果。免疫疗法已经开启了实体瘤治疗的新时代，但其有效性仍然受到限制，并带来了不必要的副作用。在分子尺度上的基因分析和操作的推动下，尖端技术的进步显示出加强这些治疗的潜力。包括CRISPR-Cas9在内的基因组编辑技术的出现，可以极大地增强癌症免疫治疗的疗效。本文综述了crispr - cas9介导的基因组编辑机制及其广泛的工具。该研究的重点是分析crispr诱导的双链断裂（DSBs）对癌症免疫治疗的影响，特别是通过基因敲低或敲入。此外，本研究还强调了利用基于CRISPR- cas9的全基因组筛选来鉴定靶点，空间CRISPR基因组学的潜力，以及CRISPR- cas9在基础研究、转化医学和临床环境中的广泛应用和难点。

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引用次数: 0

The role of phosphatidylethanolamine-binding protein (PEBP) family in various diseases: Mechanisms and therapeutic potential 磷脂酰乙醇胺结合蛋白（PEBP）家族在多种疾病中的作用：机制和治疗潜力

IF 3.2 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Progress in Biophysics & Molecular Biology

Pub Date : 2025-04-10 DOI: 10.1016/j.pbiomolbio.2025.04.002

Yeying Teng , Haiping Xue , Xiaoliang Deng , Yanqun Luo , Tao Wu

This article focuses on the phosphatidylethanolamine-binding protein (PEBP) family proteins, detailing PEBP1 and PEBP4 due to limited information on PEBP2 and PEBP3, in cellular signaling pathways and research in a spectrum of pathologies, including diverse cancers, metabolic disorders, immunological diseases and a subset of organ-specific diseases. It outlines the mechanisms through which PEBP1 and PEBP4 regulate essential signaling pathways that are critical for cellular processes such as proliferation, apoptosis, and metastasis. Recent advancements have shown further understanding of these proteins' roles in pathophysiology and their potential as future therapeutic targets. The findings suggest that the impact of PEBP1 and PEBP4 on the course of different diseases has underscored their potential for more in-depth medical research and novel clinically targeted therapies.

由于PEBP2和PEBP3的信息有限，本文重点关注磷脂酰乙醇胺结合蛋白（PEBP）家族蛋白，详细介绍了PEBP1和PEBP4在细胞信号通路和病理谱中的研究，包括各种癌症、代谢紊乱、免疫疾病和一部分器官特异性疾病。它概述了PEBP1和PEBP4调控重要信号通路的机制，这些信号通路对细胞增殖、凋亡和转移等过程至关重要。最近的进展表明这些蛋白在病理生理中的作用及其作为未来治疗靶点的潜力有了进一步的了解。研究结果表明，PEBP1和PEBP4对不同疾病病程的影响强调了它们在更深入的医学研究和新的临床靶向治疗方面的潜力。

引用次数: 0

Advances in the study of epithelial mesenchymal transition in cancer progression: Role of miRNAs 癌症进展中上皮间充质转化的研究进展：miRNAs 的作用。

IF 3.2 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Progress in Biophysics & Molecular Biology

Pub Date : 2025-04-02 DOI: 10.1016/j.pbiomolbio.2025.04.001

Jia Zhang , Runting Yin , Yongwang Xue , Rong Qin , Xuequan Wang , Shuming Wu , Jun Zhu , Yan-Shuang Li , Cai Zhang , Yuan Wei

Epithelial-mesenchymal transition (EMT) has been extensively studied for its roles in tumor metastasis, the generation and maintenance of cancer stem cells and treatment resistance. Epithelial mesenchymal plasticity allows cells to switch between various states within the epithelial-mesenchymal spectrum, resulting in a mixed epithelial/mesenchymal phenotypic profile. This plasticity underlies the acquisition of multiple malignant features during cancer progression and poses challenges for EMT in tumors. MicroRNAs (miRNAs) in the microenvironment affect numerous signaling processes through diverse mechanisms, influencing physiological activities. This paper reviews recent advances in EMT, the role of different hybrid states in tumor progression, and the important role of miRNAs in EMT. Furthermore, it explores the relationship between miRNA-based EMT therapies and their implications for clinical practice, discussing how ongoing developments may enhance therapeutic outcomes.

上皮间充质转化（Epithelial-mesenchymal transition， EMT）因其在肿瘤转移、肿瘤干细胞的产生和维持以及治疗耐药性中的作用而被广泛研究。上皮间充质可塑性允许细胞在上皮-间充质谱内的各种状态之间切换，从而形成混合的上皮/间充质表型。这种可塑性是癌症进展过程中多种恶性特征获得的基础，并对肿瘤的EMT提出了挑战。微环境中的MicroRNAs （miRNAs）通过多种机制影响众多信号过程，影响生理活动。本文综述了EMT的最新进展，不同杂交状态在肿瘤进展中的作用，以及mirna在EMT中的重要作用。此外，它探讨了基于mirna的EMT疗法及其对临床实践的影响之间的关系，讨论了正在进行的发展如何提高治疗结果。

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引用次数: 0

Myelin: A possible proton capacitor for energy storage during sleep and energy supply during wakefulness 髓磷脂：一种可能的质子电容器，用于睡眠时的能量储存和清醒时的能量供应。

IF 3.2 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Progress in Biophysics & Molecular Biology

Pub Date : 2025-03-28 DOI: 10.1016/j.pbiomolbio.2025.03.001

Alessandro Maria Morelli , Ann Saada , Felix Scholkmann

There are several physiological reasons why biological organisms sleep. One key one concerns brain metabolism. In our article we discuss the role of metabolism in myelin, based on the recent discovery that myelin contains mitochondrial components that enable the production of adenosine triphosphate (ATP) via oxidative phosphorylation (OXPHOS). These mitochondrial components in myelin probably originate from vesiculation of the mitochondrial membranes in form from mitochondrial derived vesicles (MDVs). We hypothesize that myelin acts as a proton capacitor, accumulating energy in the form of protons during sleep and converting it to ATP via OXPHOS during wakefulness. Empirical evidence supporting our hypothesis is discussed, including data on myelin metabolic activity, MDVs, and allometric scaling between white matter volume and sleep duration in mammals.

生物有机体睡眠有几个生理原因。其中一个关键因素与大脑代谢有关。在我们的文章中，我们讨论了代谢在髓磷脂中的作用，基于最近的发现，髓磷脂含有线粒体成分，能够通过氧化磷酸化（OXPHOS）产生三磷酸腺苷（ATP）。髓磷脂中的这些线粒体成分可能起源于线粒体膜的囊泡形成，其形式是线粒体源性囊泡。我们假设髓磷脂充当质子电容器，在睡眠时以质子形式积累能量，并在清醒时通过OXPHOS将其转化为ATP。本文讨论了支持我们假设的经验证据，包括哺乳动物髓磷脂代谢活性、mdv和白质体积与睡眠时间之间的异速缩放数据。

引用次数: 0

Cell detachment: A review of techniques, challenges, and opportunities for advancing biomedical research and applications 细胞分离：关于推进生物医学研究和应用的技术、挑战和机遇的综述。

IF 3.2 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Progress in Biophysics & Molecular Biology

Pub Date : 2025-02-27 DOI: 10.1016/j.pbiomolbio.2025.02.004

Polina Vertegel , Pavel Milkin , Anton Murashko , Mikhail Parker , Kristina Peranidze , Natalia Emashova , Sergiy Minko , Vladimir Reukov

Culturing living cells outside the body is a complex process involving various techniques. Despite advances, harvesting cells remains challenging, especially in light of new emerging and scaled-up cell culture technologies. Enzymatic adherent cell harvesting is the most used and robust technology but can harm cells. Non-enzymatic detachment methods offer advantages but also present challenges. Thermo-responsive polymers require precise control of the molecular characteristics and thickness of the thermoresponsive films, which makes this method less robust and more expensive. This review highlights the importance of controlling harvested cell quality and its relationship to cell binding and detachment mechanisms. Many alternative methods have not been extensively analyzed, and their impact on cell quality beyond standard viability assays is not yet known. Developing robust cell harvesting methods for bioreactor microcarriers is a rapidly growing challenge as the cell manufacturing industry expands. Microcarriers with stimuli-responsive coatings face challenges similar to those observed for laboratory-scale cell dishes and bring an additional aspect of the need for microbead recycling consideration. All that together underlines the importance of the research in biomaterials and biotechnology for cell manufacturing.

体外培养活细胞是一个涉及多种技术的复杂过程。尽管取得了进步，但收获细胞仍然具有挑战性，特别是在新兴和大规模细胞培养技术的背景下。酶贴壁细胞收集是最常用和最强大的技术，但可能对细胞造成伤害。非酶分离方法具有优势，但也存在挑战。热响应聚合物需要精确控制分子特性和热响应膜的厚度，这使得这种方法不那么可靠，而且更昂贵。这篇综述强调了控制收获细胞质量及其与细胞结合和脱离机制的关系的重要性。许多替代方法尚未被广泛分析，它们对细胞质量的影响超出标准活力测定尚不清楚。随着细胞制造行业的扩张，为生物反应器微载体开发稳健的细胞收集方法是一个迅速增长的挑战。具有刺激响应涂层的微载体面临着类似于实验室规模的细胞培养皿所观察到的挑战，并且带来了对微珠回收考虑的额外需求。所有这些都强调了生物材料和生物技术研究对细胞制造的重要性。

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引用次数: 0

Advancing targeted therapies in pancreatic cancer: Leveraging molecular abberrations for therapeutic success 推进胰腺癌靶向治疗：利用分子畸变获得治疗成功。

IF 3.2 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Progress in Biophysics & Molecular Biology

Pub Date : 2025-02-21 DOI: 10.1016/j.pbiomolbio.2025.02.003

Tanvi Gupta , Mohd Murtaza

Pancreatic cancer is one of the most deadly with poor prognosis and overall survival rate due to the dense stroma in the tumors which often is challenging for the delivery of drug to penetrate deep inside the tumor bed and usually results in the progression of cancer. The conventional treatment such as chemotherapy, radiotherapy or surgery shows a minimal benefit in the survival due to the drug resistance, poor penetration, less radiosensitivity or recurrence of tumor. There is an urgent demand to develop molecular-level targeted therapies to achieve therapeutic efficacy in the pancreatic ductal adenocarcinoma (PDAC) patients. The precision oncology focuses on the unique attributes of the patient such as epigenome, proteome, genome, microbiome, lifestyle and diet habits which contributes to promote oncogenesis. The targeted therapy helps to target the mutated proteins responsible for controlling growth, division and metastasis of tumor in the cancer cells. It is very important to consider all the attributes of the patient to provide the suitable personalized treatment to avoid any severe side effects. In this review, we have laid emphasis on the precision medicine; the utmost priority is to improve the survival of cancer patients by targeting molecular mutations through transmembrane proteins, inhibitors, signaling pathways, immunotherapy, gene therapy or the use of nanocarriers for the delivery at the tumor site. It will become beneficial therapeutic window to be considered for the advanced stage pancreatic cancer patients to prolong their survival rate.

胰腺癌是最致命的肿瘤之一，由于肿瘤间质致密，药物难以渗透到肿瘤床的深处，往往导致癌症的进展，预后和总生存率都很差。由于耐药、穿透性差、放射敏感性低或肿瘤复发，化疗、放疗或手术等传统治疗方法对生存的益处很小。为了提高胰腺导管腺癌（PDAC）患者的治疗效果，迫切需要开发分子水平的靶向治疗方法。精准肿瘤学关注患者的独特属性，如表观基因组、蛋白质组、基因组、微生物组、生活方式和饮食习惯，这些都有助于促进肿瘤的发生。靶向治疗有助于靶向癌细胞中负责控制肿瘤生长、分裂和转移的突变蛋白。考虑患者的所有属性以提供合适的个性化治疗以避免任何严重的副作用是非常重要的。在这篇综述中，我们重点介绍了精准医疗；当务之急是通过跨膜蛋白、抑制剂、信号通路、免疫治疗、基因治疗或使用纳米载体在肿瘤部位递送靶向分子突变，提高癌症患者的生存率。这将成为晚期胰腺癌患者延长生存率值得考虑的有益治疗窗口。

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