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A Model for the Binding of Fluorescently Labeled Anti-Human CD4 Monoclonal Antibodies to CD4 Receptors on Human Lymphocytes. 人淋巴细胞上荧光标记的抗人CD4单克隆抗体与CD4受体结合的模型。
IF 1.5 4区 工程技术 Q3 INSTRUMENTS & INSTRUMENTATION Pub Date : 2018-12-14 DOI: 10.1002/HTTPS://DOI.ORG/10.6028/JRES.123.022
Lili Wang, A. Gaigalas, P. DeRose
The CD4 glycoprotein is a component of the T cell receptor complex which plays an important role in the human immune response. This manuscript describes the measurement and modeling of the binding of fluorescently labeled anti-human CD4 monoclonal antibodies (mAb; SK3 clone) to CD4 receptors on the surface of human peripheral blood mononuclear cells (PBMC). CD4 mAb fluorescein isothiocyanate (FITC) and CD4 mAb allophycoerythrin (APC) conjugates were obtained from commercial sources. Four binding conditions were performed, each with the same PBMC sample and different CD4 mAb conjugate. Each binding condition consisted of the PBMC sample incubated for 30 min in labeling solutions containing progressively larger concentrations of the CD4 mAb-label conjugate. After the incubation period, the cells were re-suspended in PBS-based buffer and analyzed using a flow cytometer to measure the mean fluorescence intensity (MFI) of the labeled cell populations. A model was developed to estimate the equilibrium concentration of bound CD4 mAb-label conjugates to CD4 receptors on PBMC. A set of parameters was obtained from the best fit of the model to the measured MFI data and the known number of CD4 receptors on PBMC surface. Divalent and monovalent binding had to be invoked for the APC and FITC CD4 mAb conjugates, respectively. This suggests that the mAb binding depends on the size of the label, which has significant implications for quantitative flow cytometry. The study supports the National Institute of Standards and Technology program to develop quantitative flow cytometry measurements.
CD4糖蛋白是T细胞受体复合物的一种成分,在人类免疫反应中起着重要作用。本文描述了荧光标记的抗人CD4单克隆抗体(mAb;SK3克隆)与人外周血单核细胞(PBMC)表面CD4受体结合的测量和建模。CD4mAb异硫氰酸荧光素(FITC)和CD4mAb别藻红蛋白(APC)缀合物从商业来源获得。进行四种结合条件,每种条件具有相同的PBMC样品和不同的CD4mAb缀合物。每种结合条件由PBMC样品在含有逐渐更大浓度的CD4mAb标记缀合物的标记溶液中孵育30分钟组成。孵育期后,将细胞重新悬浮在基于PBS的缓冲液中,并使用流式细胞仪进行分析,以测量标记细胞群的平均荧光强度(MFI)。开发了一个模型来估计PBMC上与CD4受体结合的CD4mAb标记缀合物的平衡浓度。从模型与测量的MFI数据和PBMC表面上已知数量的CD4受体的最佳拟合中获得一组参数。APC和FITC CD4 mAb缀合物必须分别调用二价和单价结合。这表明mAb的结合取决于标记的大小,这对定量流式细胞术具有重要意义。这项研究支持美国国家标准与技术研究所开发定量流式细胞术测量的项目。
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引用次数: 3
Industrial Wireless End-to-End Measurements and Impacts in a Gas-Sensing Scenario. 气体传感场景中的工业无线端到端测量和影响。
IF 1.5 4区 工程技术 Q3 INSTRUMENTS & INSTRUMENTATION Pub Date : 2018-12-11 DOI: 10.1002/HTTPS://DOI.ORG/10.6028/JRES.123.023
M. Kashef, R. Candell
Industrial wireless is a potential networking solution in many scenarios due to its flexibility and ease of communications in harsh environments. Industrial wireless in gas-sensing and air-quality monitoring applications is essential when wired communications cannot perform the task safely and effectively. A major example of such environments is confined spaces where attaching mobile gas sensors with wires is a major concern for safety and cannot be deployed in some cases. At the National Institute of Standards and Technology (NIST), we developed an end-to-end characterization method for industrial wireless networks. We employed this characterization method to study the end-to-end error and delay performance for a confined-space gas-sensing scenario. We have built the scenario using the NIST industrial wireless test bed, which includes ISA100.11a wireless devices, a channel emulator, and a high-performance programmable logic controller (PLC), where the physical process is simulated. In this work, we studied the effects of the size of the confined space, the relaying, input signal rate, and the impact of the existing workers in the confined space.
工业无线由于其在恶劣环境下的灵活性和通信便利性,在许多情况下都是一种潜在的网络解决方案。当有线通信不能安全有效地执行任务时,工业无线在气体传感和空气质量监测应用中是必不可少的。这种环境的一个主要例子是密闭空间,在这些空间中,用电线连接移动气体传感器是安全的主要问题,在某些情况下无法部署。在美国国家标准与技术研究所(NIST),我们为工业无线网络开发了端到端表征方法。我们采用这种表征方法来研究密闭空间气体传感场景的端到端误差和延迟性能。我们使用NIST工业无线测试平台构建了该场景,该平台包括ISA100.11a无线设备、信道模拟器和高性能可编程逻辑控制器(PLC),其中物理过程进行了模拟。在这项工作中,我们研究了密闭空间的大小、继电器、输入信号速率以及密闭空间中现有工作人员的影响。
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引用次数: 1
Industrial Wireless End-to-End Measurements and Impacts in a Gas-Sensing Scenario. 工业无线端到端测量和气体传感场景的影响。
IF 1.5 4区 工程技术 Q3 INSTRUMENTS & INSTRUMENTATION Pub Date : 2018-12-11 eCollection Date: 2018-01-01 DOI: 10.6028/jres.123.023
Mohamed Kashef, Richard Candell

Industrial wireless is a potential networking solution in many scenarios due to its flexibility and ease of communications in harsh environments. Industrial wireless in gas-sensing and air-quality monitoring applications is essential when wired communications cannot perform the task safely and effectively. A major example of such environments is confined spaces where attaching mobile gas sensors with wires is a major concern for safety and cannot be deployed in some cases. At the National Institute of Standards and Technology (NIST), we developed an end-to-end characterization method for industrial wireless networks. We employed this characterization method to study the end-to-end error and delay performance for a confined-space gas-sensing scenario. We have built the scenario using the NIST industrial wireless test bed, which includes ISA100.11a wireless devices, a channel emulator, and a high-performance programmable logic controller (PLC), where the physical process is simulated. In this work, we studied the effects of the size of the confined space, the relaying, input signal rate, and the impact of the existing workers in the confined space.

工业无线由于其在恶劣环境下的灵活性和通信便利性,在许多情况下都是一种潜在的网络解决方案。当有线通信不能安全有效地执行任务时,工业无线在气体传感和空气质量监测应用中是必不可少的。这种环境的一个主要例子是密闭空间,在这些空间中,用电线连接移动气体传感器是安全的主要问题,在某些情况下无法部署。在美国国家标准与技术研究所(NIST),我们为工业无线网络开发了端到端表征方法。我们采用这种表征方法来研究密闭空间气体传感场景的端到端误差和延迟性能。我们使用NIST工业无线测试平台构建了该场景,该平台包括ISA100.11a无线设备、信道模拟器和高性能可编程逻辑控制器(PLC),其中物理过程进行了模拟。在这项工作中,我们研究了密闭空间的大小、继电器、输入信号速率以及密闭空间中现有工作人员的影响。
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引用次数: 1
How to Define the Units of the Revised SI Starting from Seven Constants with Fixed Numerical Values. 如何从具有固定数值的七个常数出发,定义修订版 SI 的单位。
IF 1.5 4区 工程技术 Q3 INSTRUMENTS & INSTRUMENTATION Pub Date : 2018-12-03 eCollection Date: 2018-01-01 DOI: 10.6028/jres.123.021
Richard S Davis

As part of a revision to the International System of Units (SI) approved in 2018 and to take effect in May 2019, the seven base units will be defined by giving fixed numerical values to seven defining constants. This article shows how the definitions of all seven base units can be derived efficiently from the defining constants, with the result appearing as a table. The table's form makes evident a number of connections between the defining constants and the base units. Appendices show how the same methodology could have been used to define the same base units in the present SI, as well as the mathematics that underpins the methodology. Since the base units are now defined in terms of constants, then all units in the SI are now defined in terms of those constants.

作为 2018 年批准并于 2019 年 5 月生效的国际单位制(SI)修订工作的一部分,将通过为七个定义常数赋予固定数值来定义七个基本单位。本文展示了如何从定义常数中高效导出所有七个基本单位的定义,并以表格形式显示结果。表格的形式表明了定义常数和基本单位之间的一些联系。附录展示了如何使用同样的方法来定义现行国际单位制中的相同基本单位,以及该方法的数学基础。既然基本单位现在是用常数来定义的,那么现在国际单位制中的所有单位都是用这些常数来定义的。
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引用次数: 0
Implementation of an Effective Bond Energy Formalism in the Multicomponent Calphad Approach. 多分量calphhad方法中有效键能形式的实现。
IF 1.5 4区 工程技术 Q3 INSTRUMENTS & INSTRUMENTATION Pub Date : 2018-11-26 eCollection Date: 2018-01-01 DOI: 10.6028/jres.123.020
Nathalie Dupin, Ursula R Kattner, Bo Sundman, Mauro Palumbo, Suzana G Fries

Most models currently used for complex phases in the calculation of phase diagrams (Calphad) method are based on the compound energy formalism. The way this formalism is presently used, however, is prone to poor extrapolation behavior in higher-order systems, especially when treating phases with complex crystal structures. In this paper, a partition of the Gibbs energy into effective bond energies, without changing its configurational entropy expression, is proposed, thereby remarkably improving the extrapolation behavior. The proposed model allows the use of as many sublattices as there are occupied Wyckoff sites and has great potential for reducing the number of necessary parameters, thus allowing shorter computational time. Examples for face centered cubic (fcc) ordering and the σ phase are given.

目前用于复杂相图计算(Calphad)方法的模型大多基于复合能量形式。然而,目前使用的这种形式方法在高阶系统中容易出现较差的外推行为,特别是在处理具有复杂晶体结构的相时。本文提出了在不改变构型熵表达式的情况下,将吉布斯能划分为有效键能的方法,从而显著改善了外推行为。所提出的模型允许使用尽可能多的子格,因为有占用的Wyckoff站点,并且有很大的潜力减少必要参数的数量,从而允许更短的计算时间。给出了面心立方(fcc)有序和σ相位的例子。
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引用次数: 8
Gain Calibration of Current-to-Voltage Converters. 电流-电压转换器的增益校准。
IF 1.5 4区 工程技术 Q3 INSTRUMENTS & INSTRUMENTATION Pub Date : 2018-11-19 DOI: 10.1002/HTTPS://DOI.ORG/10.6028/JRES.123.019
T. Larason, C. Cameron Miller
Current-to-voltage converters are used in many photometric and radiometric applications. The calibration of current-to-voltage converters at a few input currents is not always sufficient to understand the linearity and the bias of a device. Many devices have structure deviating from a linear response over the operating range of a gain setting. Measurement services that rely on these devices now have decreased uncertainties to a level that requires quantifying the uncertainties and understanding how they propagate. The National Institute of Standards and Technology has developed a system to calibrate the current-to-voltage conversion factor or "gain" and offset of these devices for direct current photocurrents. The equipment used for the calibration is described here, and the results and uncertainties are discussed.
电流-电压转换器用于许多光度和辐射测量应用。在少量输入电流下对电流-电压转换器进行校准并不总是足以理解器件的线性度和偏置。在增益设定的工作范围内,许多器件的结构偏离线性响应。依赖于这些设备的测量服务现在已经将不确定性降低到需要量化不确定性并了解它们如何传播的水平。美国国家标准与技术研究所开发了一套系统,用于校准这些设备的直流光电流的电流-电压转换因子或“增益”和偏移。这里描述了用于校准的设备,并讨论了结果和不确定度。
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引用次数: 1
Gain Calibration of Current-to-Voltage Converters. 电流-电压转换器的增益校准。
IF 1.5 4区 工程技术 Q3 INSTRUMENTS & INSTRUMENTATION Pub Date : 2018-11-19 eCollection Date: 2018-01-01 DOI: 10.6028/jres.123.019
Thomas Larason, C Cameron Miller

Current-to-voltage converters are used in many photometric and radiometric applications. The calibration of current-to-voltage converters at a few input currents is not always sufficient to understand the linearity and the bias of a device. Many devices have structure deviating from a linear response over the operating range of a gain setting. Measurement services that rely on these devices now have decreased uncertainties to a level that requires quantifying the uncertainties and understanding how they propagate. The National Institute of Standards and Technology has developed a system to calibrate the current-to-voltage conversion factor or "gain" and offset of these devices for direct current photocurrents. The equipment used for the calibration is described here, and the results and uncertainties are discussed.

电流-电压转换器用于许多光度和辐射测量应用。在少量输入电流下对电流-电压转换器进行校准并不总是足以理解器件的线性度和偏置。在增益设定的工作范围内,许多器件的结构偏离线性响应。依赖于这些设备的测量服务现在已经将不确定性降低到需要量化不确定性并了解它们如何传播的水平。美国国家标准与技术研究所开发了一套系统,用于校准这些设备的直流光电流的电流-电压转换因子或“增益”和偏移。这里描述了用于校准的设备,并讨论了结果和不确定度。
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引用次数: 0
A Model of a Wireless Factory Work-Cell Using the Systems Modeling Language. 使用系统建模语言的无线工厂工作单元模型。
IF 1.3 4区 工程技术 Q3 INSTRUMENTS & INSTRUMENTATION Pub Date : 2018-10-25 eCollection Date: 2018-01-01 DOI: 10.6028/jres.123.018
Richard Candell
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引用次数: 0
A Model of a Wireless Factory Work-Cell Using the Systems Modeling Language. 基于系统建模语言的无线工厂工作单元模型。
IF 1.5 4区 工程技术 Q3 INSTRUMENTS & INSTRUMENTATION Pub Date : 2018-10-25 DOI: 10.1002/HTTPS://DOI.ORG/10.6028/JRES.123.018
R. Candell
Wireless technology is a key enabler of the vision of the future factory work-cell. Such a work-cell will operate autonomously with a high degree of mobility enabled by wireless technology. This paper describes the work-cell using the Systems Modeling Language (SysML). Using SysML the structural and parametric characteristics of the work-cell are described. Our model provides the architectural components and performance constraints of the work-cell in which wireless is used for a significant portion of connectivity. It identifies the structural components, interfaces, and data flows. Parametric characteristics that impact work-cell performance are included in the model. Using this model, industrial wireless networking requirements and work-cell behaviors may be developed and performance limits may be evaluated. The SysML model presented here is developed using MagicDraw 18.5 by No Magic.
无线技术是未来工厂工作单元愿景的关键推动者。这种工作单元将通过无线技术实现高度的移动性,自主运行。本文利用系统建模语言(SysML)对工作单元进行了描述。利用SysML描述了工作单元的结构特征和参数特征。我们的模型提供了工作单元的体系结构组件和性能约束,其中无线用于连接的重要部分。它标识了结构组件、接口和数据流。影响工作单元性能的参数特征包含在模型中。使用该模型,可以开发工业无线网络需求和工作单元行为,并可以评估性能限制。这里介绍的SysML模型是使用No Magic的MagicDraw 18.5开发的。
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引用次数: 1
Calibration Fluids and Calibration Equations: How Choices May Affect the Results of Density Measurements Made with U-Tube Densimeters. 校准流体和校准方程:选择如何影响用u型管密度计进行密度测量的结果。
IF 1.5 4区 工程技术 Q3 INSTRUMENTS & INSTRUMENTATION Pub Date : 2018-10-09 DOI: 10.1002/HTTPS://DOI.ORG/10.6028/JRES.123.017
S. Outcalt
Data on the calibration fluids water and toluene individually, and the combination of those two sets of data, were correlated with two different equations (resulting in six sets of calibration equation parameters) to analyze data measured with a vibrating-tube densimeter on the lubricants pentaerythritol tetrapentanoate (POE5), pentaerythritol tetraheptanoate (POE7), and pentaerythritol tetranonanoate (POE9) at temperatures from 270 K to 470 K and pressures from 0.5 MPa to 50 MPa. The objective was to explore how the calculated densities of the lubricants would differ based on the calibration equations and calibration fluid(s). The viscosities of the measured lubricants are much greater than those of the calibration fluids, and because there has long been a question of how measurements of higher viscosity fluids are affected when measured with a vibrating-tube densimeter, combinations of calibration fluid(s) and equations were tested to explore the role they play in obtaining accurate results. For the lubricants studied herein, more accurate results were obtained with a calibration fitted to multiple calibration fluids, while the consistency of results was more equation dependent.
将校准流体水和甲苯的单独数据以及这两组数据的组合与两个不同的方程相关联(产生六组校准方程参数),以分析用振动管密度计测量的润滑剂季戊四醇四戊酸酯(POE5)、,和季戊四醇四壬酸酯(POE9)在270K至470K的温度和0.5MPa至50MPa的压力下。目的是探索润滑油的计算密度如何根据校准方程和校准液而不同。测量的润滑剂的粘度远大于校准液的粘度,由于长期以来一直存在使用振动管密度计测量高粘度流体时如何影响测量的问题,因此对校准液和方程的组合进行了测试,以探索它们在获得准确结果中所起的作用。对于本文研究的润滑剂,在将校准液与多种校准液相匹配的情况下获得了更准确的结果,而结果的一致性更依赖于方程。
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引用次数: 8
期刊
Journal of Research of the National Institute of Standards and Technology
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