We evaluate whether cumulative photosynthesis (An,C) could predict the time of harvest in strawberry better than light (IC) or temperature (Ta,C) as forecast indices. We monitored the daily dynamics of flowering, fruit set, and harvest in over 2,700 fruits across the growing season in a greenhouse in Japan. Photosynthesis was calculated using a biochemical model, and An,C calculated by totalling the cumulative rate from flowering to harvest in each fruit. IC and Ta,C were calculated by totalling daily cumulative light intensity and average temperature. A new index (D) was defined as the normalised variation of An,C, IC, and Ta,C, and a statistical analysis was conducted on the means for each index. The results showed that An,C had the lowest D (0.114), suggesting that it was a better index for predicting the time of harvest. This study suggests the importance of considering the accumulation of photosynthates in individual fruit, which cannot be achieved by IC or Ta,C, these results can be used to improve the forecasting and optimisation of yield. However, further research is required to substantiate these claims.
{"title":"Effect of microclimate and photosynthesis on strawberry reproductive growth in a greenhouse: using cumulative leaf photosynthesis as an index to predict the time of harvest","authors":"Shintaro Ono, Daisuke Yasutake, Kensuke Kimura, I Kengo, Yoshiya Teruya, Kota Hidaka, Gaku Yokoyama, Tomoyoshi Hirota, Masaharu Kitano, Takashi Okayasu, Yukio Ozaki","doi":"10.1080/14620316.2023.2254759","DOIUrl":"https://doi.org/10.1080/14620316.2023.2254759","url":null,"abstract":"We evaluate whether cumulative photosynthesis (An,C) could predict the time of harvest in strawberry better than light (IC) or temperature (Ta,C) as forecast indices. We monitored the daily dynamics of flowering, fruit set, and harvest in over 2,700 fruits across the growing season in a greenhouse in Japan. Photosynthesis was calculated using a biochemical model, and An,C calculated by totalling the cumulative rate from flowering to harvest in each fruit. IC and Ta,C were calculated by totalling daily cumulative light intensity and average temperature. A new index (D) was defined as the normalised variation of An,C, IC, and Ta,C, and a statistical analysis was conducted on the means for each index. The results showed that An,C had the lowest D (0.114), suggesting that it was a better index for predicting the time of harvest. This study suggests the importance of considering the accumulation of photosynthates in individual fruit, which cannot be achieved by IC or Ta,C, these results can be used to improve the forecasting and optimisation of yield. However, further research is required to substantiate these claims.","PeriodicalId":54808,"journal":{"name":"Journal of Horticultural Science & Biotechnology","volume":"82 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136071456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Okra is an important vegetable crop of the Malvaceae family and is infected by varying numbers of viruses of the genus Begomovirus. Regardless of the importance of the crop, very little consideration has been given to its genetic improvement. RNA interference (RNAi), a potent biotechnological tool, is known to control Begomovirus in many crops. For the implementation of successful RNAi, there is a need for an efficient genetic transformation system in okra. In the present study, we developed a procedure for Agrobacterium-mediated tissue culture–dependent regeneration of okra plants for the application of RNAi. Eleven transgenic okra RNAi plants were regenerated by utilising hypocotyls as explants. Transformed plants were screened with hygromycin at the regeneration stage and the presence of transgenes (AC1, AC2 & AC4 codes for replication-associated protein, transcriptional activator protein and suppressor of PTGS) in putative transformed plants was confirmed by polymerase chain reaction (PCR). Wild and transgenic lines were challenged with a dimeric Begomovirus clone or viruliferous whiteflies and the level of resistance was estimated with quantitative real-time reverse transcriptase PCR (qRT-PCR) by utilising viral gene–specific primers. The resistant transgenic lines accumulated very low titres of viral gene products according to the qRT-PCR assays compared to the control plants. This is the first report of tissue culture–mediated RNAi-derived resistance in okra against Begomovirus infection.
{"title":"Effective <i>Agrobacterium</i>-mediated genetic transformation of okra (<i>Abelmoschus esculentus</i> L.) and generation of RNAi plants resistant to <i>Begomovirus</i> infecting okra","authors":"Nity Sharma, Navraj Kaur Sarao, Prashant Mohanpuria, Abhishek Sharma","doi":"10.1080/14620316.2023.2251978","DOIUrl":"https://doi.org/10.1080/14620316.2023.2251978","url":null,"abstract":"Okra is an important vegetable crop of the Malvaceae family and is infected by varying numbers of viruses of the genus Begomovirus. Regardless of the importance of the crop, very little consideration has been given to its genetic improvement. RNA interference (RNAi), a potent biotechnological tool, is known to control Begomovirus in many crops. For the implementation of successful RNAi, there is a need for an efficient genetic transformation system in okra. In the present study, we developed a procedure for Agrobacterium-mediated tissue culture–dependent regeneration of okra plants for the application of RNAi. Eleven transgenic okra RNAi plants were regenerated by utilising hypocotyls as explants. Transformed plants were screened with hygromycin at the regeneration stage and the presence of transgenes (AC1, AC2 & AC4 codes for replication-associated protein, transcriptional activator protein and suppressor of PTGS) in putative transformed plants was confirmed by polymerase chain reaction (PCR). Wild and transgenic lines were challenged with a dimeric Begomovirus clone or viruliferous whiteflies and the level of resistance was estimated with quantitative real-time reverse transcriptase PCR (qRT-PCR) by utilising viral gene–specific primers. The resistant transgenic lines accumulated very low titres of viral gene products according to the qRT-PCR assays compared to the control plants. This is the first report of tissue culture–mediated RNAi-derived resistance in okra against Begomovirus infection.","PeriodicalId":54808,"journal":{"name":"Journal of Horticultural Science & Biotechnology","volume":"50 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136071443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-17DOI: 10.1080/14620316.2023.2211593
Harriet M. Berry, Nan Zhou, Daniel V. Rickett, Charles J. Baxter, Eugenia M. A. Enfissi, Paul D. Fraser
The red colouration of ripe chilli pepper fruit is due to the presence of the carotenoid capsanthin. 1-D-deoxy-xylulose 5-phosphate synthase (DXS) and phytoene synthase(s) (PSY) are influential biosynthetic steps in the carotenoid pathway. A panel of chilli accessions for varying fruit colour intensity revealed the correlation of carotenoid content with PSY and DXS transcript levels. The PSY and DXS genes were sequenced from high and low carotenoid genotypes to deduce potential allelic variation. PSY-1 and -2 showed tissue-specific expression, with PSY-1 and -2 expressed in chromoplast and chloroplast containing tissues, respectively. Protein modelling, phylogenetic analysis and transcription factor analysis were conducted to understand how the allelic variation identified could affect carotenoid biosynthesis and regulation, and subsequently colour intensity phenotype. A candidate mutation located in the transit peptide of the PSY-1 protein sequence was identified in the low colour intensity genotype. Sequence variation within the promoter region of the DXS gene present in the high colour intensity genotype was determined in the glyceraldehyde 3-phosphate binding motif (GAPF). These data can be exploited to develop tools and resources for the breeding of high colour intensity chilli pepper fruit.
成熟辣椒果实的红色是由于类胡萝卜素辣椒素的存在。1- d -脱氧木糖5-磷酸合成酶(DXS)和植物烯合成酶(PSY)是类胡萝卜素途径中重要的生物合成步骤。一组不同果实颜色强度的辣椒材料揭示了类胡萝卜素含量与PSY和DXS转录水平的相关性。对高、低类胡萝卜素基因型的PSY和DXS基因进行测序，推断潜在的等位基因变异。PSY-1和psy -2表现出组织特异性表达，PSY-1和psy -2分别在含染色质和含叶绿体的组织中表达。通过蛋白质建模、系统发育分析和转录因子分析来了解所鉴定的等位基因变异如何影响类胡萝卜素的生物合成和调控，以及随后的颜色强度表型。在低颜色强度基因型中发现了一个位于PSY-1蛋白序列传递肽的候选突变。高颜色强度基因型DXS基因启动子区域的序列变异在甘油醛3-磷酸结合基序(GAPF)中确定。这些数据可用于开发高色强辣椒果实育种的工具和资源。
{"title":"The characterisation of phytoene synthase-1 and 2, and 1-D-deoxy-xylulose 5-phosphate synthase genes from red chilli pepper (<i>Capsicum annuum</i>)","authors":"Harriet M. Berry, Nan Zhou, Daniel V. Rickett, Charles J. Baxter, Eugenia M. A. Enfissi, Paul D. Fraser","doi":"10.1080/14620316.2023.2211593","DOIUrl":"https://doi.org/10.1080/14620316.2023.2211593","url":null,"abstract":"The red colouration of ripe chilli pepper fruit is due to the presence of the carotenoid capsanthin. 1-D-deoxy-xylulose 5-phosphate synthase (DXS) and phytoene synthase(s) (PSY) are influential biosynthetic steps in the carotenoid pathway. A panel of chilli accessions for varying fruit colour intensity revealed the correlation of carotenoid content with PSY and DXS transcript levels. The PSY and DXS genes were sequenced from high and low carotenoid genotypes to deduce potential allelic variation. PSY-1 and -2 showed tissue-specific expression, with PSY-1 and -2 expressed in chromoplast and chloroplast containing tissues, respectively. Protein modelling, phylogenetic analysis and transcription factor analysis were conducted to understand how the allelic variation identified could affect carotenoid biosynthesis and regulation, and subsequently colour intensity phenotype. A candidate mutation located in the transit peptide of the PSY-1 protein sequence was identified in the low colour intensity genotype. Sequence variation within the promoter region of the DXS gene present in the high colour intensity genotype was determined in the glyceraldehyde 3-phosphate binding motif (GAPF). These data can be exploited to develop tools and resources for the breeding of high colour intensity chilli pepper fruit.","PeriodicalId":54808,"journal":{"name":"Journal of Horticultural Science & Biotechnology","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135862202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-05-04DOI: 10.1080/14620316.2018.1500086
Chen Minmin, Zhang Jianjun, Zhou Yin, Liu Shuigen, Fang Xiaofen, Y. Liuyan, Guanshen Yuan, Zhang Yongchun
ABSTRACTHighly efficient somatic embryogenesis (SE) initiation systems are crucial for the clonal propagation and genetic transformation of Lilium Oriental × Trumpet hybrid. To elucidate the underlying mechanism of SE induction, we conducted de novo transcriptome sequencing of the roots at four different times in relation to SE initiation (0 days, control; 20 days, embryogenic callus induction (ECI) 1; 40 days, ECI2; 55 days, ECI3). A total of 4621, 7582, and 7427 differentially expressed genes (DEGs) were identified from the ECI1 vs. control, ECI2 vs. control, and ECI3 vs. control comparisons, respectively. The gene-enrichment analysis revealed 54 significant DEGs. Further analysis showed that the transcript levels of the auxin-related genes ARF9/15/16, the abscisic acid-related (ABA-related) genes PYL4/9, and stress-related transcription factors, including WRKY26, MYB36, NAC100, bHLH2/5/18/112, and HSP90, were increased at the ECI1 stage. ABI5 can influence the expression of stress-responsive genes as a...
{"title":"Transcriptome analysis of Lilium Oriental × Trumpet hybrid roots reveals auxin-related genes and stress-related genes involved in picloram-induced somatic embryogenesis induction","authors":"Chen Minmin, Zhang Jianjun, Zhou Yin, Liu Shuigen, Fang Xiaofen, Y. Liuyan, Guanshen Yuan, Zhang Yongchun","doi":"10.1080/14620316.2018.1500086","DOIUrl":"https://doi.org/10.1080/14620316.2018.1500086","url":null,"abstract":"ABSTRACTHighly efficient somatic embryogenesis (SE) initiation systems are crucial for the clonal propagation and genetic transformation of Lilium Oriental × Trumpet hybrid. To elucidate the underlying mechanism of SE induction, we conducted de novo transcriptome sequencing of the roots at four different times in relation to SE initiation (0 days, control; 20 days, embryogenic callus induction (ECI) 1; 40 days, ECI2; 55 days, ECI3). A total of 4621, 7582, and 7427 differentially expressed genes (DEGs) were identified from the ECI1 vs. control, ECI2 vs. control, and ECI3 vs. control comparisons, respectively. The gene-enrichment analysis revealed 54 significant DEGs. Further analysis showed that the transcript levels of the auxin-related genes ARF9/15/16, the abscisic acid-related (ABA-related) genes PYL4/9, and stress-related transcription factors, including WRKY26, MYB36, NAC100, bHLH2/5/18/112, and HSP90, were increased at the ECI1 stage. ABI5 can influence the expression of stress-responsive genes as a...","PeriodicalId":54808,"journal":{"name":"Journal of Horticultural Science & Biotechnology","volume":"9 1","pages":"317-330"},"PeriodicalIF":1.9,"publicationDate":"2019-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82335597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ABSTRACTPotassium (K +) is one of the many elements necessary for plant growth and development, and is the only one that plants need to have a higher concentration of cation. In order to cope with ...
{"title":"Molecular cloning of potassium transporter gene, MaHAK5 of mulberry (Morus alba L.) and gene expression and biochemistry analysis under potassium stress","authors":"Dandan Chen, Ackon Justice Afriyie, Ruixue Li, Kotoka Dominic, Long Li, Rongfang Li, Weiguo Zhao","doi":"10.1080/14620316.2018.1470909","DOIUrl":"https://doi.org/10.1080/14620316.2018.1470909","url":null,"abstract":"ABSTRACTPotassium (K +) is one of the many elements necessary for plant growth and development, and is the only one that plants need to have a higher concentration of cation. In order to cope with ...","PeriodicalId":54808,"journal":{"name":"Journal of Horticultural Science & Biotechnology","volume":"94 1","pages":"130-136"},"PeriodicalIF":1.9,"publicationDate":"2019-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/14620316.2018.1470909","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43382503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-02DOI: 10.1080/14620316.2018.1450097
D. Lang, P. Fei, G. Cao, X. Jia, Y. T. Li, X. Zhang
ABSTRACTA sand culture experiment was conducted to determine the effects of Si on the growth and endogenous hormone concentrations (indole-3-acetic acid [IAA], gibberellic acid [GA3], and abscisic acid [ABA]) in Glycyrrhiza uralensis seedlings under 100 mM NaCl stress. Si time-dependently improved the growth and increased the biomass of seedlings, and the effect was greater at 20 days than that at 10 days after Si treatment. The effects of Si on the growth parameters and biomass were dependent on Si concentration, that is, these parameters increased with increasing Si concentration. Si also increased the levels of endogenous hormones in G. uralensis seedlings in a concentration-dependent manner. The effects on IAA and GA3 increased with increasing Si concentrations, and the effect on ABA was higher at 2 mM Si than that at the other Si concentrations. Furthermore, the effects of Si on IAA and GA3 were greater at 10 days than those at 20 days after the treatment, whereas the effect on ABA was greater at 20 ...
{"title":"Silicon promotes seedling growth and alters endogenous IAA, GA3 and ABA concentrations in Glycyrrhiza uralensis under 100 mM NaCl stress","authors":"D. Lang, P. Fei, G. Cao, X. Jia, Y. T. Li, X. Zhang","doi":"10.1080/14620316.2018.1450097","DOIUrl":"https://doi.org/10.1080/14620316.2018.1450097","url":null,"abstract":"ABSTRACTA sand culture experiment was conducted to determine the effects of Si on the growth and endogenous hormone concentrations (indole-3-acetic acid [IAA], gibberellic acid [GA3], and abscisic acid [ABA]) in Glycyrrhiza uralensis seedlings under 100 mM NaCl stress. Si time-dependently improved the growth and increased the biomass of seedlings, and the effect was greater at 20 days than that at 10 days after Si treatment. The effects of Si on the growth parameters and biomass were dependent on Si concentration, that is, these parameters increased with increasing Si concentration. Si also increased the levels of endogenous hormones in G. uralensis seedlings in a concentration-dependent manner. The effects on IAA and GA3 increased with increasing Si concentrations, and the effect on ABA was higher at 2 mM Si than that at the other Si concentrations. Furthermore, the effects of Si on IAA and GA3 were greater at 10 days than those at 20 days after the treatment, whereas the effect on ABA was greater at 20 ...","PeriodicalId":54808,"journal":{"name":"Journal of Horticultural Science & Biotechnology","volume":"94 1","pages":"87-93"},"PeriodicalIF":1.9,"publicationDate":"2019-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/14620316.2018.1450097","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41875677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-02DOI: 10.1080/14620316.2018.1449671
G. Jat, A. D. Munshi, T. Behera, H. Choudhary, P. Dash, Amarnath Ravindran, S. Kumari
Gynoecious is an important economic trait of cucumber for determinant of earliness and yield, yet genetic mechanism is not well understood for this trait. The experiment was conducted using F₂ mapping population by crossing of PPC-2, a gynoecious and parthenocarpic line with Pusa Uday (monoecious and non-parthenocarpic cultivar). Out of 179 SSR markers screened, 39 markers differentiated the gynoecious and monoecious parents. However, only 17 markers were segregating with F₂ mapping population, those were used for genotyping and linkage map analysis and these markers were placed along with F locus on chromosome 6 covering a total distance of 100.4cM. The SSR markers, SSR13251 and UW020605 were found to be closely linked to gynoecious (F) locus at 1.0 and 4.5 cM, respectively. The segregation of F₂ population of PPC-2 × Pusa Uday and GPC-1 × Punjab Naveen and test crosses for sex type herein suggested that single dominant gene controlled the gynoecious sex expression in cucumber particularly in gynoecious genotypes PPC-2 and GPC-1. Therefore, the monogenic dominant nature of gynoecious sex identified in the present experiment and SSR markers closely linked to the F locus will be useful in marker-assisted backcross breeding for transfering gynoecious trait into horticulturally desirable varieties.
{"title":"Genetics and molecular mapping of gynoecious (F) locus in cucumber (Cucumis sativus L.)","authors":"G. Jat, A. D. Munshi, T. Behera, H. Choudhary, P. Dash, Amarnath Ravindran, S. Kumari","doi":"10.1080/14620316.2018.1449671","DOIUrl":"https://doi.org/10.1080/14620316.2018.1449671","url":null,"abstract":"Gynoecious is an important economic trait of cucumber for determinant of earliness and yield, yet genetic mechanism is not well understood for this trait. The experiment was conducted using F₂ mapping population by crossing of PPC-2, a gynoecious and parthenocarpic line with Pusa Uday (monoecious and non-parthenocarpic cultivar). Out of 179 SSR markers screened, 39 markers differentiated the gynoecious and monoecious parents. However, only 17 markers were segregating with F₂ mapping population, those were used for genotyping and linkage map analysis and these markers were placed along with F locus on chromosome 6 covering a total distance of 100.4cM. The SSR markers, SSR13251 and UW020605 were found to be closely linked to gynoecious (F) locus at 1.0 and 4.5 cM, respectively. The segregation of F₂ population of PPC-2 × Pusa Uday and GPC-1 × Punjab Naveen and test crosses for sex type herein suggested that single dominant gene controlled the gynoecious sex expression in cucumber particularly in gynoecious genotypes PPC-2 and GPC-1. Therefore, the monogenic dominant nature of gynoecious sex identified in the present experiment and SSR markers closely linked to the F locus will be useful in marker-assisted backcross breeding for transfering gynoecious trait into horticulturally desirable varieties.","PeriodicalId":54808,"journal":{"name":"Journal of Horticultural Science & Biotechnology","volume":"94 1","pages":"24-32"},"PeriodicalIF":1.9,"publicationDate":"2019-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/14620316.2018.1449671","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49358475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-02DOI: 10.1080/14620316.2018.1444514
Yan-Lan Guo, G. Niu, T. Starman, M. Gu
A greenhouse study was conducted to evaluate the growth and flowering of Easter lily (Lilium longiflorum Thunb.) grown in a commercial substrate (Sunshine Mix #1) amended with biochar at 0 (control...
对复活节百合（Lilium longiflorum Thunb.）在0（对照。。。
{"title":"Growth and development of Easter lily in response to container substrate with biochar","authors":"Yan-Lan Guo, G. Niu, T. Starman, M. Gu","doi":"10.1080/14620316.2018.1444514","DOIUrl":"https://doi.org/10.1080/14620316.2018.1444514","url":null,"abstract":"A greenhouse study was conducted to evaluate the growth and flowering of Easter lily (Lilium longiflorum Thunb.) grown in a commercial substrate (Sunshine Mix #1) amended with biochar at 0 (control...","PeriodicalId":54808,"journal":{"name":"Journal of Horticultural Science & Biotechnology","volume":"94 1","pages":"80-86"},"PeriodicalIF":1.9,"publicationDate":"2019-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/14620316.2018.1444514","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44316067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-02DOI: 10.1080/14620316.2018.1450647
Z. Pakkish, M. R. H. Poor, M. A. Nasab, H. Asghari
The effects of different applications of 24-epi-brassinolideon the physiology and yield of ‘Owhadi’ pistachio trees was investigated in Iran over an off and on year. Plants were treated by 24-epi-b...
在伊朗研究了不同施用24-外延油菜内酯对‘Owhadi’开心树生理和产量的影响。24-epi-b…
{"title":"Brassinolides have a small effect on vegetative growth, and increase reproductive growth of pistachio trees Cv. Owhadi","authors":"Z. Pakkish, M. R. H. Poor, M. A. Nasab, H. Asghari","doi":"10.1080/14620316.2018.1450647","DOIUrl":"https://doi.org/10.1080/14620316.2018.1450647","url":null,"abstract":"The effects of different applications of 24-epi-brassinolideon the physiology and yield of ‘Owhadi’ pistachio trees was investigated in Iran over an off and on year. Plants were treated by 24-epi-b...","PeriodicalId":54808,"journal":{"name":"Journal of Horticultural Science & Biotechnology","volume":"94 1","pages":"118-122"},"PeriodicalIF":1.9,"publicationDate":"2019-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/14620316.2018.1450647","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46003192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-02DOI: 10.1080/14620316.2018.1454266
F. Yan, Lihu Wang, Xingjuan Zheng, Zhi Luo, Jiurui Wang, Mengjun Liu
ABSTRACT‘Chenguang’ is the first released tetraploid cultivar (2n = 4x = 48) of Chinese jujube (Ziziphus jujuba Mill.) induced by colchicine. The purpose of this study was to create triploid germplasms by using ‘Chenguang’ as a parent. The controlled crosses between autotetraploid ‘Chenguang’ and diploid ‘Dongzao’ were carried out from 2013 to 2015 by maintaining the 2 parents in a nylon net and employing bees as pollinator. A total of 606 fruits were produced, yet only 17 seeds were obtained from ‘Dongzao’ and none from ‘Chenguang’. Direct sowing of the seeds in greenhouse showed a higher seedling rate than embryo culture. Four progeny plants were eventually obtained in the greenhouse and all of them were confirmed as authentic hybrids by SSR marker technique. At last, 3 triploids and 1 diploid were identified by flow cytometric analysis. Consequently, tetraploid ‘Chenguang’ with high pollen vitality and pollen number per anther could be used as a reliable 2n pollen provider, and the 2x × 4x cross combin...
{"title":"Acquisition of triploid germplasms by controlled hybridisation between diploid and tetraploid in Chinese jujube","authors":"F. Yan, Lihu Wang, Xingjuan Zheng, Zhi Luo, Jiurui Wang, Mengjun Liu","doi":"10.1080/14620316.2018.1454266","DOIUrl":"https://doi.org/10.1080/14620316.2018.1454266","url":null,"abstract":"ABSTRACT‘Chenguang’ is the first released tetraploid cultivar (2n = 4x = 48) of Chinese jujube (Ziziphus jujuba Mill.) induced by colchicine. The purpose of this study was to create triploid germplasms by using ‘Chenguang’ as a parent. The controlled crosses between autotetraploid ‘Chenguang’ and diploid ‘Dongzao’ were carried out from 2013 to 2015 by maintaining the 2 parents in a nylon net and employing bees as pollinator. A total of 606 fruits were produced, yet only 17 seeds were obtained from ‘Dongzao’ and none from ‘Chenguang’. Direct sowing of the seeds in greenhouse showed a higher seedling rate than embryo culture. Four progeny plants were eventually obtained in the greenhouse and all of them were confirmed as authentic hybrids by SSR marker technique. At last, 3 triploids and 1 diploid were identified by flow cytometric analysis. Consequently, tetraploid ‘Chenguang’ with high pollen vitality and pollen number per anther could be used as a reliable 2n pollen provider, and the 2x × 4x cross combin...","PeriodicalId":54808,"journal":{"name":"Journal of Horticultural Science & Biotechnology","volume":"94 1","pages":"123-129"},"PeriodicalIF":1.9,"publicationDate":"2019-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/14620316.2018.1454266","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46704858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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