Genetic contamination through the cultivation of indigenous crops in their native range has become a concern for nature conservation and agriculture alike. The formation of hybrids has negative evolutionary consequences for wild populations and selective breeding consequences for agriculture. To address some of these questions, we investigate gene flow between wild populations and cultivated honeybush – genus Cyclopia Vent. (Fabaceae) – which has commercial value in the tea industry globally. Through genetic data, pollen movement can indirectly be estimated, but complementary data on pollinator flight distances is necessary to validate this. Movement between flowers of cultivated and wild Cyclopia by the only pollinators, Xylocopa bees, was investigated using mark-release-recapture and radio-tagging. Hand-pollination experiments established whether crosses within and between species from cultivated and wild populations can produce viable offspring. Xylocopa bees forage indiscriminately between cultivated and wild Cyclopia during single foraging bouts with a foraging distance over one kilometre and a range covering up to ∼24 000 m2. Crossing experiments done by hand showed that viable hybrid seeds can be produced between C. subternata and C. genistoides as well as between C. subternata and C. maculata. Considering the distances moved by the pollinators, longevity of Cyclopia pollen, and the ability of hybrid plants to form, some gene flow through pollen transfer between cultivated and wild Cyclopia plants is likely. To minimize genetic homogenization and the subsequent loss of unique species and alleles, a protocol guiding safe Cyclopia planting distances, effect of Cyclopia species movement outside of their native range for agriculture, and detailed ploidy studies are urgently needed to guide cultivation of indigenous crops in the native range.
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