The Indian pangolin (Manis crassicaudata) is an endangered, nocturnal mammal threatened by habitat loss and illegal trade. This study explores the spatio-temporal effects of land use and land cover (LULC) changes on their occurrence in six districts of Khyber Pakhtunkhwa, Pakistan, from 2003 to 2023. It examines the impact of habitat type, elevation, soil pH, Landsat indices (NDVI, NDMI, NDWI), and climate variables (precipitation, mean temperature). Using multitemporal Landsat imagery and binomial generalized linear models (GLMs) at the site level (2 km line-transects, 50 m strip width; 0.10k m2 per site), we analyzed changes in LULC and their influence on pangolin 2023 presence and regional extirpation (present in 2003 but absent in 2023), which was supported by field surveys and local reports. The investigation highlights significant shifts in habitat suitability amid rapid urbanization and landscape transformations. Field surveys and local reports confirm the presence of pangolins in all varieties of habitats. We modeled site-level presence/absence and a binary extirpation outcome (present in 2003, absent in 2023), screened predictors for collinearity (|r| ≥ 0.7), tested residual spatial autocorrelation (Moran’s I; not significant), and evaluated discrimination with spatial cross-validation. The final 2023 presence model retained elevation, soil pH, NDWI, and temperature; the extirpation model retained ΔNDMI, ΔNDWI, Δprecipitation, Δtemperature, elevation, and soil pH. Spatial cross-validated AUCs were low (≈0.49–0.50), and odds-ratio 95 % CIs overlapped 1, indicating that vegetation indices and coarse climate alone did not strongly explain present occupancy or local loss at the analyzed scale. Land-use and land-cover analysis exposed a rapid urban expansion, with built-up areas increasing by 1,130.47 km2 and barren lands declining by 1,274.14 km2. Forested habitats that supported pangolins in 2003 showed reduced suitability by 2023, with the species disappearing from 35 of 102 sites. These regional extirpations showed the impact of habitat changes and urbanization on pangolin persistence. The findings underscore the need for wildlife corridors, habitat restoration, and sustainable land-use planning to mitigate further biodiversity loss.
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