Helminthologia最新文献

Fasciolosis is a parasitic disease that affects both humans and animals. Due to parasitic resistance to chemical drugs, there has been a growing focus on studying the anthelmintic properties of plants. In vitro, studies were conducted on the ovicidal activity of ethanolic extracts of 29 plants against eggs of liver flukes from cattle. Plants were selected due to their availability and previous literature reports. Each plant's ethanolic extract was tested at a concentration of 20 % of the raw extract. The incubation period was 15 days at 28°C for all treated eggs, while control samples were treated with ethanol and water. Mortality levels of eggs ranged from 0 to 100 %. Moringa oleifera, Ananas comosus, and Foeniculum vulgare caused the highest mortality levels of 100 %, 100 %, and 90 %, respectively, followed by Cymbopogon nardus and Artemisia afra, which caused mortality levels of 62 % and 60 %, respectively. The plant extracts were then used in a concentration-response experiment using 5 %, 10 %, and 20 % extracts. Extracts from Moringa oleifera and Ananas comosus showed the highest ovicidal activity at the three concentrations, followed by F. vulgare, C. nardus, and A. afra. At the 5 % concentration, M. oleifera and A. comosus extracts were both ovicidal, with rates of 83 % and 80 %, respectively.

This is the report of mixed infection of an entomopathogenic nematode and a free-living bacteriophage nematode both invading Galleria mellonella larvae, apparently showing strong intraguild competition. The entomopathogenic nematodes isolated in this study were classified as Heterorhabditis kankabi n. sp., and Metarhabditis rainai through light and electronic microscopy, sequencing of ITS and 28S ribosomal DNA regions, and phylogenetic analyses. The bacteriophage nematode M. rainai was recognized as a facultative necromenic nematode, as it consumed the food resources obtained by entomopathogenic nematodes, directly affecting the life cycle of the later by stopping their development and forcing them to leave G. mellonella in search of new hosts.

Great cormorants (Phalacrocorax carbo) are widespread piscivorous birds, which cause huge economic losses in the fish fauna worldwide. As a consequence of their predatory behaviour, they serve as definitive hosts for many digenean trematodes, and their digestive systems usually contain several parasite species. Between 2019 and 2022, 131 bird carcasses were collected from Biharugra (Hungary) as culling on the cormorant population. Their digestive systems were subjected to parasitological examination. The studied organs (131 intestines, 44 stomachs and 21 pharynxes) were opened, and their contents were settled in water, filtered, and sorted under microscope. For species identification, sequence analysis of the ITS region was performed. Of the 131 birds, 118 were infected by tapeworms or nematodes, 105 with trematodes, and 10 were parasite-free. The vast majority of obtained sequences (57 of the 105 trematodes) belonged to the genus Petasiger (43/57) and Hysteromorpha triloba (13/57). A single specimen of Metorchis sp. (1/57) as a zoonotic trematode was recorded during our survey. The results confirmed the high trematodes prevalence of the examined cormorants. However, most of them are not considered as human pathogens.

Morphological and metrical analyses of monogeneans from the gills of Antarctic rockcod Notothenia coriiceps revealed the presence of a new species, Pseudobenedeniella johnstoni sp. n. Fresh specimens of monogeneans collected from N. coriiceps from the vicinity of Galindez Island, Argentine Islands, West Antarctica were examined morphologically, by molecular analysis and Energy Dispersive X-ray analysis (EDXA). The new species differs from Pseudobenedeniella branchialis Timofeeva, Gaevskaya, Kovaljova, 1987 by the different shapes of anterior and posterior hamuli, the presence of a pronounced sickle-shaped blade of the anterior hamulus and its characteristic wide (wing-like) shaft, serrated on one side, smaller posterior hamulus with a distinct short and broad shaft that widens and is serrated distally, more extensive dimensions of the penis and its pear-like shape, smaller vagina diameter, ovoid egg shape with more pointed anterior pole and blunt posterior pole and long coiled filament as well as different type species of the host in a geographically distant type locality. Genetic data accompanied the description, and phylogenetic analyses inferred that the new species clustered with monogenean species positioned under the family Capsalidae. Molecular data were generated for the 18S and 28S genes of the ribosomal RNA of P. johnstoni sp. n. to provide the first molecular analysis for Pseudobenedeniella species. EDXA for a species of Pseudobenedeniella was offered for the first time.

Diseases caused by intestinal parasites impose a substantial burden on the population of middle-income countries, including Pakistan. This research aimed to assess the risk factors for intestinal parasites in Malakand, Pakistan, school children. Three hundred sixty stool samples were collected from school children, of which 140 (39 %) tested positive for helminth, including taenids. The wet mount preparation in saline/iodine/methods was used for stool examination. The GraphPad Prism of version 5 was used to analyze the data, and the P value was considered significant when it was less than 0.05 % (at 95 % CI). Males were more infected at 40.7 % compared to female students at 33.3 %. (P>0.005 at 95 % CI). Among the helminth Ascaris lumbricoides was the most prevalent 30.71 % (n=43), taeniid species 22.85 % (n=32), Hookworms12.14 % (n=17), Hymenolepis nana 10 % (n=14), Enterobius vermicularis 7.85 % (n=1), Hymenolupis diminuta 6.42 % (n=9), and Trichuris trichiura 5.71 % (n=8) were reported. Age-wise prevalence was noted as the students aged 11 to 13 years were highly infected at 57.4 %, followed by 8 to 10 years at 48.8 %, while the least prevalence was noted in the age 5 to 7 years at 45.5 %. (P>0.005 at 95 % CI). Regarding the association of intestinal parasitic infection and nutritional status of the students, 53.0 % had 10 to 11 inches in diameter upper arm circumference, and 49.4 % had 8 to 9 inches in upper arm circumference (P>0.05). Information on the students' locality and intestinal parasitic infection shows that students in rural areas were more infected, 54.1 %, compared to urban areas, 41.1 % (P value is 0.023 at 95 % CI). Based on the pattern of infection, 19.44 % (n=70/360) were single, 10.5 % (n=38/360) double, 6.11 % (n=22/360), and 2.77 % (n=10/360) quadruple infections had been recorded. The symptoms were abdominal pain, constipation, nausea or vomiting, stomach pain, blotting, and Diarrhea appearing after the taeniid infection. We conclude that since deworming is insufficient to control parasitic illnesses, there is a need for widespread campaigns to raise awareness about children's health and hygiene, as well as the necessity for the creation of efficient poverty control programs.

Dirofilariasis is a zoonotic infection that humans acquire by the bite of infected mosquitoes. The male reproductive system is relatively rare affected by this disease. We presented a case of dirofilariasis of the scrotum from Bulgaria. The patient noticed a small painless nodule in his scrotum with no other pathological findings. The tumor-like lesion was surgically removed. Dirofilariasis was not suspected before this procedure, but the diagnosis was made on histological examination of the extracted tissue. Surgical removal of the helminth is the radical treatment of this disease. Dirofilariasis of the scrotum has a good prognosis as patients fully recover after the extraction of the parasite.

Infections with hookworms (Necator americanus and Ancylostoma duodenale) remain a major public health problem in low- and middle-income countries. However, the information about the distribution of each species is inaccurate in many countries since their traditional diagnosis is based only on the identification of eggs in stool under a microscope. We aimed to identify the prevalence of hookworm species using morphological stools to identify L3 larvae to gain insights into the distribution of both species in five regions of Cameroon. Samples were collected from schoolchildren in five regions and 34 subdivisions of Cameroon and examined using the Kato-Katz method. We randomly selected a total of 157 samples among hookworm's positive stool samples. They were cultured using the Harada-Mori test-tube technique. The morphological identification of a total of 8063 isolated hookworm filariform larvae L3 was conducted following established criteria. The sensitivity rate to the Harada-Mori technique was 58 %. Among the 8063 L3 larvae identified during this study, 230 (2.95 %) of L3 larvae were identified as A. duodenale, and 7833 (97.15 %) of L3 larvae were identified as N. americanus. A. duodenale was observed only in the Mouanko subdivision in the Littoral region. The complementary use of the Kato Katz and the Harada-Mori culture techniques to screen hookworm infections contributes to the differentiation of N. americanus and A. duodenale as the two hookworm species in Cameroon. An extended molecular study in the localities where only N. americanus has been identified is necessary to reach more conclusions on the distribution of hookworm species in Cameroon.

The development of turkey farming is significantly restrained by parasitic diseases, which have become widespread and cause significant economic losses to specialized- and homestead farms. This study aimed to determine the prevalence of endoparasites in turkeys of different age groups under different maintenance systems and the nature of patho-anatomical changes depending on the course of the disease. A total of 1,869 samples of fecal from turkeys were studied. From turkeys kept in poultry houses, 925 fecal samples and 944 fecal samples from free-range turkeys were examined. In turkeys that were kept in a poultry house, the total infection with endoparasites was 59.8 %, while in free range, it was 63.3 %. The most common in turkeys 30-60 days old from poultry houses are Histomonas meleagridis (21.5 %), Eimeria spp. (11.5 %) and Cryptosporidium baileyi (15.5 %) and in free-range turkeys - Histomonas meleagridis (15.6 %), Eimeria spp. (7.2 %) and Cryptosporidium baileyi (10.8 %). Cryptosporidium baileyi was not observed in turkeys 360 days old or older. Tetratrichomonas gallinarum was most frequently recorded in turkeys 90 - 120 days old from poultry houses (3.8 %) and turkeys 150 - 180 days old in free range (3.7 %). Ascaridia dissimilis is most common in free-range turkeys 90 - 120 days old (11.8 %) and turkeys 150 - 180 days old from poultry houses (9.5 %). Heterakis gallinarum was primarily recorded in turkeys 150 - 180 days old under both systems of keeping with a value range from 16.2 to 17.2 %. Infestation of 150 - 180-day-old free-range turkeys by cestodes Davainea meleagridis and Raillietina echinobothrida was 15.0 % and 12.9 %, respectively. Thus, the diversity and prevalence of gastrointestinal tract invasions depend on the age and housing system of the poultry.

A 25-year-old intensive monoculture of cucumber (Cucumis sativus 'Monolit F1') has been chosen for monitoring the effect of continuous fertilization, drip irrigation and various soil disinfection on free-living nematodes in soil. Densities, relative abundance of nematode genera, trophic groups and c-p classes, in addition, numerous nematological indices were determined besides certain soil properties. Concerning the nematological indices, all the values proved a stressed, depleted and unstable environment with degraded food web condition. The exaggerated application of fertilizers through irrigation caused high salinity values (0.62 %) which is much higher than the indicated toxicity threshold (0.4 %) for cultivated plants. The number of individuals of the bacterivore Acrobeloides and Cuticularia genera, which have a very high stress tolerance, was outstanding in the area. Besides juveniles, the appearance of Meloidogyne incognita males was also recorded which could lead to a change in the genetic composition of the population to adapt to environmental stressors. All these changes may result from salt stress indicated by intensive drip irrigation and the use of artificial fertilizers.

Background: Infections with intestinal parasites are the major cause of infectious disease globally and have been described as a public health issue in developing countries.

Objective: This study aimed to determine prevalence of intestinal parasitic infections and their associated factors among the population of Erbil province in Iraq over the period 2011-2021.

Methods: The results of 614455 stool examinations of all public health facilities of the province were collected from the register in the directorate of preventive health affairs and the data were analyzed.

Result: The overall prevalence of intestinal parasitic infections was 4.24 % in the province. The total number of reported cases of intestinal parasites decreased from 4352 for 2011 to 1728 for 2018 and then increased to 2014 cases for 2021. Nearly half of the intestinal parasitic infections were detected among age group of 15 to 44 years. The prevalence of intestinal parasitic infections was higher in males (65.73 %) than females (34.27 %). The prevalence of intestinal parasitic infections was 9 0.47 % for protozoan infections, while the rate of helminth infection was lower (9 .53 %). Entamoeba histolytica/dispar were the most common parasite (60.92 %) followed by Giardia lamblia (29.54 %) and Enterobius vermicularis (8.56 %). Prevalence of infection with intestinal parasites was lowest in March and highest in February.

Conclusion: In spite of that intestinal parasitic infection rates appear to be decreasing, intestinal parasitic infection remains an important health problem in the province. Therefore, there is still a need for prevention efforts in the community.