Pub Date : 2023-06-27DOI: 10.1177/1721727x231185390
Wenhui Gong, S. Jiang, Jianbo Wang, Qian Gong, Chengxin Zhang, Shenglin Ge
Objectives: Bone marrow-derived mesenchymal stem cells (BMSCs) are considered to have potential clinical application value in the treatment of acute lung injury (ALI). Myeloid-derived growth factor (MYDGF) can promote the proliferation of stem cell. We hypothesized that MYDGF may play a role in reducing lung injury in vitro and in vivo through bone marrow mesenchymal stem cells. Methods: An in vitro model of lipopolysaccharide (LPS)(MLE-12) was established, which was divided into five groups: A: MLE-12; B: MLE-12+LPS; C: MLE-12+LPS + BMSCs; D: MLE-12+LPS + MYDGF; and E: MLE-12+LPS + BMSCs + MYDGF. A Cell Counting Kit-8 was used to detect the OD value. And an ALI model was constructed by inducing mice with a lipopolysaccharide. Forty male Balb/c mice were randomly divided into five groups: A control group; B: model group; C: LPS + BMSCs; D: LPS + MYDGF; E: LPS +BMSCs +MYDGF. Specimens were collected after 24 h. Hematoxylin-eosin (HE)-staining was performed on the tissue sections. The protein concentration in the alveolar lavage fluid was measure by bicinchoninic acid (BCA). The NF-κB, p-Akt, Bax, and Bcl-2 protein expression was detected through Western blotting, and Enzyme linked immunosorbent assay (ELISA) was used to measure the expression of serum interleukin-6, interleukin-10, and TNF-α. Results: Compared with the model group, BMSCs and MYDGF can alleviate the ALI induced by lipopolysaccharide in vitro and vivo ( p < .05). Conclusion: We found that the combined treatment effect of MYDGF and BMSCs was better than using MYDGF or BMSCs alone. We speculate that a pretreatment with MYDGF after ALI in mice may improve the survival and growth of transplanted MSCs, thereby improving the curative effect of cell transplantation.
{"title":"A study of the role of myeloid-derived growth factor on acute lung injury in vitro and In vivo","authors":"Wenhui Gong, S. Jiang, Jianbo Wang, Qian Gong, Chengxin Zhang, Shenglin Ge","doi":"10.1177/1721727x231185390","DOIUrl":"https://doi.org/10.1177/1721727x231185390","url":null,"abstract":"Objectives: Bone marrow-derived mesenchymal stem cells (BMSCs) are considered to have potential clinical application value in the treatment of acute lung injury (ALI). Myeloid-derived growth factor (MYDGF) can promote the proliferation of stem cell. We hypothesized that MYDGF may play a role in reducing lung injury in vitro and in vivo through bone marrow mesenchymal stem cells. Methods: An in vitro model of lipopolysaccharide (LPS)(MLE-12) was established, which was divided into five groups: A: MLE-12; B: MLE-12+LPS; C: MLE-12+LPS + BMSCs; D: MLE-12+LPS + MYDGF; and E: MLE-12+LPS + BMSCs + MYDGF. A Cell Counting Kit-8 was used to detect the OD value. And an ALI model was constructed by inducing mice with a lipopolysaccharide. Forty male Balb/c mice were randomly divided into five groups: A control group; B: model group; C: LPS + BMSCs; D: LPS + MYDGF; E: LPS +BMSCs +MYDGF. Specimens were collected after 24 h. Hematoxylin-eosin (HE)-staining was performed on the tissue sections. The protein concentration in the alveolar lavage fluid was measure by bicinchoninic acid (BCA). The NF-κB, p-Akt, Bax, and Bcl-2 protein expression was detected through Western blotting, and Enzyme linked immunosorbent assay (ELISA) was used to measure the expression of serum interleukin-6, interleukin-10, and TNF-α. Results: Compared with the model group, BMSCs and MYDGF can alleviate the ALI induced by lipopolysaccharide in vitro and vivo ( p < .05). Conclusion: We found that the combined treatment effect of MYDGF and BMSCs was better than using MYDGF or BMSCs alone. We speculate that a pretreatment with MYDGF after ALI in mice may improve the survival and growth of transplanted MSCs, thereby improving the curative effect of cell transplantation.","PeriodicalId":55162,"journal":{"name":"European Journal of Inflammation","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44405022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
To investigate whether dexmedetomidine (Dex) can reduce the severity of tourniquet-induced lung injury. 36 patients undergoing total knee arthroplasty with a tourniquet were randomly assigned to the control (ischemia/reperfusion [I/R]) group and Dex group. Patients in the Dex group received a loading dose of Dex (0.8 μg/kg over 10 min intravenously) followed by continuous infusion of Dex (0.5 μg/kg/h intravenously) until the end of the surgery. The I/R group received an equal amount of 0.9% saline instead of Dex. The serum concentrations of tumor necrosis factor-α (TNF-α), Clara cell protein (CC-16), soluble receptor for advanced glycation end products (sRAGE), and brain-derived neurotrophic factor (BDNF) were measured and arterial blood gas analysis was performed before anesthesia and 30 min, 6 h, and 24 h after tourniquet release. In the I/R group, compared with baseline, the TNF-α, CC-16, and sRAGE concentrations were higher ( p < 0.05) and the BDNF concentration was lower ( p < 0.05) at most time points. In the Dex group, the TNF-α, CC-16, and sRAGE concentrations were lower than those in the I/R group ( p < 0.05), whereas the concentration of BDNF was higher ( p < 0.05). In the arterial blood gas analysis, the Dex group showed a significantly higher partial pressure of oxygen and arterial/alveolar oxygen tension ratio ( p < 0.05) and a significantly lower alveolar/arterial oxygen tension difference than the I/R group ( p < 0.05). Dex administration partly inhibits the release of proinflammatory cytokines, affording protection against tourniquet-induced lung injury.
{"title":"Protective effect of dexmedetomidine on Tourniquet induced lung injury in patients undergoing total knee arthroplasty: A randomized trial","authors":"Wenjie Cheng, Jizheng Zhang, Zhe Wu, Xiaohua Sun, Wanlu Ren","doi":"10.1177/1721727x231179671","DOIUrl":"https://doi.org/10.1177/1721727x231179671","url":null,"abstract":"To investigate whether dexmedetomidine (Dex) can reduce the severity of tourniquet-induced lung injury. 36 patients undergoing total knee arthroplasty with a tourniquet were randomly assigned to the control (ischemia/reperfusion [I/R]) group and Dex group. Patients in the Dex group received a loading dose of Dex (0.8 μg/kg over 10 min intravenously) followed by continuous infusion of Dex (0.5 μg/kg/h intravenously) until the end of the surgery. The I/R group received an equal amount of 0.9% saline instead of Dex. The serum concentrations of tumor necrosis factor-α (TNF-α), Clara cell protein (CC-16), soluble receptor for advanced glycation end products (sRAGE), and brain-derived neurotrophic factor (BDNF) were measured and arterial blood gas analysis was performed before anesthesia and 30 min, 6 h, and 24 h after tourniquet release. In the I/R group, compared with baseline, the TNF-α, CC-16, and sRAGE concentrations were higher ( p < 0.05) and the BDNF concentration was lower ( p < 0.05) at most time points. In the Dex group, the TNF-α, CC-16, and sRAGE concentrations were lower than those in the I/R group ( p < 0.05), whereas the concentration of BDNF was higher ( p < 0.05). In the arterial blood gas analysis, the Dex group showed a significantly higher partial pressure of oxygen and arterial/alveolar oxygen tension ratio ( p < 0.05) and a significantly lower alveolar/arterial oxygen tension difference than the I/R group ( p < 0.05). Dex administration partly inhibits the release of proinflammatory cytokines, affording protection against tourniquet-induced lung injury.","PeriodicalId":55162,"journal":{"name":"European Journal of Inflammation","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44651563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-23DOI: 10.1177/1721727x231177137
O. Mohammed, Melkitu Kassaw, Endalkachew Befekadu, L. G/Egzeabher, A. Mekonen, D. Bikila, F. Challa, A. Belay, Mistire Wolde, Kassu Desta, A. Tsegaye
Objective: Since then, the reference intervals of serum hsCRP in apparently healthy Ethiopian children are unknown. Therefore, this study aimed to determine the reference intervals of serum hsCRP for apparently healthy children. Methods: In this cross-sectional study, a total of 492 apparently healthy children aged 5–17 years were recruited from residents of Addis Ababa. Well-trained professionals took anthropometric measurements. The biochemical analysis was performed with the automatic biochemical analyzer Cobas Integra 400 Plus from Roche. The 2.5th and 97.5th percentile limits were calculated using a non-parametric method as per the Clinical Laboratory Standard Institute (CLSI) guide. A box plot was used to show the distribution of hsCRP by age and gender. Furthermore, bivariate and multivariable logistic regression analyses were carried out to evaluate factors associated with elevated hsCRP levels. Percentiles were displayed by age subgroup and sex. p-values <0.05 were considered statistically significant. Results: The study included 245 boys and 247 girls. For the age group of 5–11 years, the combined 2.5th and 97.5th percentiles, which can be used as a reference interval for children in Addis Ababa, were 0.15–6.78 mg/L, while for the older age group (12–17 years), the determined percentile was 0.14–4.40 mg/L. Likewise, the current study shows that never or occasionally eating vegetables and/or fruits (AOR = 2.28, 95% CI, 0.84–4.97), and being obese (AOR = 3.63, 95% CI, 1.14–11.53) were independent risk factors for elevated serum hsCRP concentrations. Conclusion: This study is the first to determine the reference intervals of hsCRP concentrations among apparently healthy Ethiopian children. Notable differences were observed in the established percentiles of hsCRP in comparison with published literature.
{"title":"Determination of high-sensitivity C-reactive protein reference intervals for apparently healthy children in Addis Ababa, Ethiopia","authors":"O. Mohammed, Melkitu Kassaw, Endalkachew Befekadu, L. G/Egzeabher, A. Mekonen, D. Bikila, F. Challa, A. Belay, Mistire Wolde, Kassu Desta, A. Tsegaye","doi":"10.1177/1721727x231177137","DOIUrl":"https://doi.org/10.1177/1721727x231177137","url":null,"abstract":"Objective: Since then, the reference intervals of serum hsCRP in apparently healthy Ethiopian children are unknown. Therefore, this study aimed to determine the reference intervals of serum hsCRP for apparently healthy children. Methods: In this cross-sectional study, a total of 492 apparently healthy children aged 5–17 years were recruited from residents of Addis Ababa. Well-trained professionals took anthropometric measurements. The biochemical analysis was performed with the automatic biochemical analyzer Cobas Integra 400 Plus from Roche. The 2.5th and 97.5th percentile limits were calculated using a non-parametric method as per the Clinical Laboratory Standard Institute (CLSI) guide. A box plot was used to show the distribution of hsCRP by age and gender. Furthermore, bivariate and multivariable logistic regression analyses were carried out to evaluate factors associated with elevated hsCRP levels. Percentiles were displayed by age subgroup and sex. p-values <0.05 were considered statistically significant. Results: The study included 245 boys and 247 girls. For the age group of 5–11 years, the combined 2.5th and 97.5th percentiles, which can be used as a reference interval for children in Addis Ababa, were 0.15–6.78 mg/L, while for the older age group (12–17 years), the determined percentile was 0.14–4.40 mg/L. Likewise, the current study shows that never or occasionally eating vegetables and/or fruits (AOR = 2.28, 95% CI, 0.84–4.97), and being obese (AOR = 3.63, 95% CI, 1.14–11.53) were independent risk factors for elevated serum hsCRP concentrations. Conclusion: This study is the first to determine the reference intervals of hsCRP concentrations among apparently healthy Ethiopian children. Notable differences were observed in the established percentiles of hsCRP in comparison with published literature.","PeriodicalId":55162,"journal":{"name":"European Journal of Inflammation","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42020561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-21DOI: 10.1177/1721727x231177815
Linxi Zeng, Xin Huang, Yue-ping Yao, G. Zhang
No consensus exists regarding the association between pemphigus and thyroid disease. To explore the relationship between the two conditions by synthesizing the existing data. We performed this review based on PubMed, Embase and Web of Science (from inception to 2022) and used the Newcastle-Ottawa scale for assessing the quality of studies. The outcome was represented by pooled odds ratios (ORs) with 95% confidence intervals (CI). And the publication bias and sensitivity analyses were developed. Our analyze finally included six studies containing 17,567 pemphigus patients. Overall, we revealed that pemphigus is significantly associated with hypothyroidism (OR 1.70, 95% CI 1.54–1.87), while not with thyroid cancer (OR 0.93, 95% CI 0.41–2.11) and autoimmune thyroid disease (AITD) (OR 1.64, 95% CI 0.91–2.95). In subgroup analyses, pemphigus was also not associated with Graves’ disease (OR 0.97, 95% CI 0.63–1.49) and Hashimoto’s thyroiditis (OR 1.32, 95% CI 0.88–1.97). Our results reveal a significant association between pemphigus and hypothyroidism. Considering other relevant studies, we also speculate that the prevalence of AITD is higher in pemphigus patients than in the general population.
关于天疱疮和甲状腺疾病之间的关系尚无共识。综合现有资料，探讨两者之间的关系。我们基于PubMed、Embase和Web of Science(从成立到2022年)进行了这项综述，并使用纽卡斯尔-渥太华量表来评估研究的质量。结果用95%置信区间(CI)的合并优势比(ORs)表示。并进行了发表偏倚和敏感性分析。我们的分析最终纳入了6项研究，共包含17567例天疱疮患者。总的来说，我们发现天疱疮与甲状腺功能减退显著相关(OR 1.70, 95% CI 1.54-1.87)，而与甲状腺癌(OR 0.93, 95% CI 0.41-2.11)和自身免疫性甲状腺疾病(AITD) (OR 1.64, 95% CI 0.91-2.95)无关。在亚组分析中，天疱疮也与Graves病(OR 0.97, 95% CI 0.63-1.49)和桥本甲状腺炎(OR 1.32, 95% CI 0.88-1.97)无关。我们的研究结果揭示了天疱疮和甲状腺功能减退之间的显著关联。考虑到其他相关研究，我们也推测天疱疮患者的AITD患病率高于一般人群。
{"title":"Pemphigus and thyroid disease: A systematic review and meta-analysis","authors":"Linxi Zeng, Xin Huang, Yue-ping Yao, G. Zhang","doi":"10.1177/1721727x231177815","DOIUrl":"https://doi.org/10.1177/1721727x231177815","url":null,"abstract":"No consensus exists regarding the association between pemphigus and thyroid disease. To explore the relationship between the two conditions by synthesizing the existing data. We performed this review based on PubMed, Embase and Web of Science (from inception to 2022) and used the Newcastle-Ottawa scale for assessing the quality of studies. The outcome was represented by pooled odds ratios (ORs) with 95% confidence intervals (CI). And the publication bias and sensitivity analyses were developed. Our analyze finally included six studies containing 17,567 pemphigus patients. Overall, we revealed that pemphigus is significantly associated with hypothyroidism (OR 1.70, 95% CI 1.54–1.87), while not with thyroid cancer (OR 0.93, 95% CI 0.41–2.11) and autoimmune thyroid disease (AITD) (OR 1.64, 95% CI 0.91–2.95). In subgroup analyses, pemphigus was also not associated with Graves’ disease (OR 0.97, 95% CI 0.63–1.49) and Hashimoto’s thyroiditis (OR 1.32, 95% CI 0.88–1.97). Our results reveal a significant association between pemphigus and hypothyroidism. Considering other relevant studies, we also speculate that the prevalence of AITD is higher in pemphigus patients than in the general population.","PeriodicalId":55162,"journal":{"name":"European Journal of Inflammation","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45667045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-15DOI: 10.1177/1721727x231176943
A. Shalaby, A. Ismail, Yasser Farook, Safwat M. Abdel-Aziz, Marwa M. Thabet, Abeer A Mokhtar, Azza A Fadle, Dalia A Nigm
Objectives: The aim of this study was to determine the association between neutrophils and monocytes CD 64 molecules and cases of bronchiolitis with an associated bacterial infection. Methods: Blood samples were obtained from patients younger than 2 years old diagnosed with bronchiolitis. Complete blood count (CBC), blood culture, procalcitonin, and CD64 surface expression in neutrophils and monocytes were examined using flow cytometry. We also assessed CBC, procalcitonin, and CD64 levels in neutrophils and monocytes in 31 healthy control subjects. Results: Sixty-two patients with lower respiratory tract infections were included in the first group. The patients were divided into 34 with non-bacterial infections and 28 with possible bacterial infections. We found a significant increase in the number of positive cells in the Mean Fluorescence Intensity (MFI) of both nCD64 and mCD64 in the Possible Bacterial Infection (PBI) group. Procalcitonin levels correlated with nCD64 and nCD64%, but not with mCD64. The AUC of nCD64 was 0.873 (at a cut-off point of 4489.4; the MFI had a specificity of 73% and a sensitivity of 94%). Conclusion: Neutrophil CD64 may be a marker for bacterial infection in children with bronchiolitis.
{"title":"CD64 as a diagnostic marker for bacterial infection in acute bronchiolitis","authors":"A. Shalaby, A. Ismail, Yasser Farook, Safwat M. Abdel-Aziz, Marwa M. Thabet, Abeer A Mokhtar, Azza A Fadle, Dalia A Nigm","doi":"10.1177/1721727x231176943","DOIUrl":"https://doi.org/10.1177/1721727x231176943","url":null,"abstract":"Objectives: The aim of this study was to determine the association between neutrophils and monocytes CD 64 molecules and cases of bronchiolitis with an associated bacterial infection. Methods: Blood samples were obtained from patients younger than 2 years old diagnosed with bronchiolitis. Complete blood count (CBC), blood culture, procalcitonin, and CD64 surface expression in neutrophils and monocytes were examined using flow cytometry. We also assessed CBC, procalcitonin, and CD64 levels in neutrophils and monocytes in 31 healthy control subjects. Results: Sixty-two patients with lower respiratory tract infections were included in the first group. The patients were divided into 34 with non-bacterial infections and 28 with possible bacterial infections. We found a significant increase in the number of positive cells in the Mean Fluorescence Intensity (MFI) of both nCD64 and mCD64 in the Possible Bacterial Infection (PBI) group. Procalcitonin levels correlated with nCD64 and nCD64%, but not with mCD64. The AUC of nCD64 was 0.873 (at a cut-off point of 4489.4; the MFI had a specificity of 73% and a sensitivity of 94%). Conclusion: Neutrophil CD64 may be a marker for bacterial infection in children with bronchiolitis.","PeriodicalId":55162,"journal":{"name":"European Journal of Inflammation","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41988962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-12DOI: 10.1177/1721727x231172025
Liwei Liu, Bo Liu
Hepatocellular carcinoma (HCC) is considered a classic inflammation-associated tumor that usually originates from chronic hepatitis, where an intense and chronic inflammatory response leads to the accumulation of mutations and eventually carcinogenesis under conditions of persistent liver injury. In recent years, immunotherapy for HCC has continued to evolve, as the liver is naturally filled with a large variety of immune cells, making hepatocellular carcinoma a more complex inflammatory microenvironment unlike other tumors. With a better understanding of the specific inflammatory microenvironment of HCC, there is an opportunity to try new therapeutic strategies for HCC immunotherapy. In this paper, we review the immunotherapy of primary liver cancer in terms of the correlation between ICI drugs, ACT therapy and the inflammatory microenvironment of HCC, summarize and discuss the progress and difficulties of immunotherapy of liver cancer, and provide more scientific guidance for immunotherapy of primary liver cancer.
{"title":"Inflammatory microenvironment and immunotherapy in hepatocellular carcinoma","authors":"Liwei Liu, Bo Liu","doi":"10.1177/1721727x231172025","DOIUrl":"https://doi.org/10.1177/1721727x231172025","url":null,"abstract":"Hepatocellular carcinoma (HCC) is considered a classic inflammation-associated tumor that usually originates from chronic hepatitis, where an intense and chronic inflammatory response leads to the accumulation of mutations and eventually carcinogenesis under conditions of persistent liver injury. In recent years, immunotherapy for HCC has continued to evolve, as the liver is naturally filled with a large variety of immune cells, making hepatocellular carcinoma a more complex inflammatory microenvironment unlike other tumors. With a better understanding of the specific inflammatory microenvironment of HCC, there is an opportunity to try new therapeutic strategies for HCC immunotherapy. In this paper, we review the immunotherapy of primary liver cancer in terms of the correlation between ICI drugs, ACT therapy and the inflammatory microenvironment of HCC, summarize and discuss the progress and difficulties of immunotherapy of liver cancer, and provide more scientific guidance for immunotherapy of primary liver cancer.","PeriodicalId":55162,"journal":{"name":"European Journal of Inflammation","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46051229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-08DOI: 10.1177/1721727x231175019
Zhengzheng Chen, Li Zhong, Yanyan Jiang, L. Xiao, L. Lai, Jun Cao
Objective: To investigate the effect and clinical significance of myeloid-derived suppressor cells (MDSCs) levels and serum miRNA-21 expression in advanced non-small cell lung cancer (NSCLC) patients. Methods: Advanced NSCLC patients treated at the First People’s Hospital of Tongxiang City, Zhejiang Province, between July 2021 and June 2022 were included in this study. MDSCs in the peripheral circulation were assessed by flow cytometry, and serum miRNA-21 expression was assessed by q-PCR. The effects of miRNA-21 on MDSC proliferation and ARG-1 and inducible nitric oxide synthase (iNOS) secretion from MDSCs were examined using in vitro cell experiments. In addition, the correlation between MDSC and CEA, CRP and Ki67 was analyzed. Results: Compared with those in the peripheral circulation of individuals in the control group, the expression levels of polymorphonuclear-MDSCs (PMN-MDSCs) and miRNA-21 in the peripheral circulation of NSCLC patients were significantly higher, and the two groups were significantly positively correlated. In in vitro cell experiments, miRNA-21 mimics promoted MDSC proliferation and increased ARG-1 and iNOS secretion and miRNA-21 inhibtor has an opposite result. In addition, PMN-MDSCs in the peripheral circulation of NSCLC patients were significantly positively correlated with carcinoembryonic antigen (CEA), C-reactive protein (CRP), and Ki67. Conclusion: The blood of advanced NSCLC patients contains high levels of MDSCs and miRNA-21, and the two may interact to impact on NSCLC.
{"title":"Correlation between circulating myeloid-derived suppressor cells and serum miRNA21 in non-small cell lung cancer and its clinical significance","authors":"Zhengzheng Chen, Li Zhong, Yanyan Jiang, L. Xiao, L. Lai, Jun Cao","doi":"10.1177/1721727x231175019","DOIUrl":"https://doi.org/10.1177/1721727x231175019","url":null,"abstract":"Objective: To investigate the effect and clinical significance of myeloid-derived suppressor cells (MDSCs) levels and serum miRNA-21 expression in advanced non-small cell lung cancer (NSCLC) patients. Methods: Advanced NSCLC patients treated at the First People’s Hospital of Tongxiang City, Zhejiang Province, between July 2021 and June 2022 were included in this study. MDSCs in the peripheral circulation were assessed by flow cytometry, and serum miRNA-21 expression was assessed by q-PCR. The effects of miRNA-21 on MDSC proliferation and ARG-1 and inducible nitric oxide synthase (iNOS) secretion from MDSCs were examined using in vitro cell experiments. In addition, the correlation between MDSC and CEA, CRP and Ki67 was analyzed. Results: Compared with those in the peripheral circulation of individuals in the control group, the expression levels of polymorphonuclear-MDSCs (PMN-MDSCs) and miRNA-21 in the peripheral circulation of NSCLC patients were significantly higher, and the two groups were significantly positively correlated. In in vitro cell experiments, miRNA-21 mimics promoted MDSC proliferation and increased ARG-1 and iNOS secretion and miRNA-21 inhibtor has an opposite result. In addition, PMN-MDSCs in the peripheral circulation of NSCLC patients were significantly positively correlated with carcinoembryonic antigen (CEA), C-reactive protein (CRP), and Ki67. Conclusion: The blood of advanced NSCLC patients contains high levels of MDSCs and miRNA-21, and the two may interact to impact on NSCLC.","PeriodicalId":55162,"journal":{"name":"European Journal of Inflammation","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43844025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-27DOI: 10.1177/1721727x231173161
Jiayue Qing, Hong-mei Zhou, Liye Hu, Zhi-peng Zhu
Objective: Endoplasmic reticulum stress (ERS) occurs throughout the pathological process of myocardial ischaemia reperfusion injury (MIRI); however, the key mediators involved in the biological processes of ERS or that can influence the outcomes of ischaemia reperfusion injury (IRI) are unclear. To identify the differentially expressed genes (DEGs) encoding promising mediators in rat hearts in the context of MIRI, gene profiles were mined through transcriptome analysis. Methods: By searching the Gene Expression Omnibus (GEO) database, a suitable GEO dataset was identified in the context of MIRI with 24 h reperfusion after 45 min ischaemia in rats. DEGs were extracted and analysed with R studio, and the properties of those DEGs were elucidated by utilizing protein‒protein-interaction (PPI), Cytoscape, GO and KEGG bioinformatics techniques. The potential hub genes in the first cluster of the DEG dataset were retrieved and verified by basal experiments. Results: By searching the target dataset GSE122020, 1647 mRNAs, including 790 upregulated and 857 downregulated genes, were found in the datasets with criteria of | fold change ≥ 1.2 and p value <0.05. The upregulated and downregulated genes were primarily involved in the “positive regulation of cytokine production”, “regulation of inflammatory response” and “response to pheromone” biological processes. Most of them were mainly correlated with the MAPK, Ras and calcium signalling pathways. In particular, 15 DEGs were found to be involved in the ERS signalling pathways, including Bfar, Itpr1, Srpx, Hspa1b, Usp25, Jun, Casp12, Eif2ak2, Casp4, Trim25, Ifng, Atf3, Hspa1a, Ppp1r15a, and Creb3. Through analysis with the MCODE plugin in Cytoscape, six genes, Atf3, Ppp1r15a, Casp12, Eif2ak2, Ifng, and Hspa1a, were identified as hub genes by the K-means algorithm from Cluster 1. In the in vivo MIRI model, the six genes mentioned above exhibited significant mRNA overexpression in the left anterior descending coronary artery ligation (LAD) model in rats. Conclusions: Altogether, the present study identified six potential DEGs involved in ERS induced by MIRI, which could be therapeutic targets in dealing with IRI-related ERS.
{"title":"The key mediators involved in myocardial endoplasmic reticulum stress induced by ischaemia reperfusion injury in rats","authors":"Jiayue Qing, Hong-mei Zhou, Liye Hu, Zhi-peng Zhu","doi":"10.1177/1721727x231173161","DOIUrl":"https://doi.org/10.1177/1721727x231173161","url":null,"abstract":"Objective: Endoplasmic reticulum stress (ERS) occurs throughout the pathological process of myocardial ischaemia reperfusion injury (MIRI); however, the key mediators involved in the biological processes of ERS or that can influence the outcomes of ischaemia reperfusion injury (IRI) are unclear. To identify the differentially expressed genes (DEGs) encoding promising mediators in rat hearts in the context of MIRI, gene profiles were mined through transcriptome analysis. Methods: By searching the Gene Expression Omnibus (GEO) database, a suitable GEO dataset was identified in the context of MIRI with 24 h reperfusion after 45 min ischaemia in rats. DEGs were extracted and analysed with R studio, and the properties of those DEGs were elucidated by utilizing protein‒protein-interaction (PPI), Cytoscape, GO and KEGG bioinformatics techniques. The potential hub genes in the first cluster of the DEG dataset were retrieved and verified by basal experiments. Results: By searching the target dataset GSE122020, 1647 mRNAs, including 790 upregulated and 857 downregulated genes, were found in the datasets with criteria of | fold change ≥ 1.2 and p value <0.05. The upregulated and downregulated genes were primarily involved in the “positive regulation of cytokine production”, “regulation of inflammatory response” and “response to pheromone” biological processes. Most of them were mainly correlated with the MAPK, Ras and calcium signalling pathways. In particular, 15 DEGs were found to be involved in the ERS signalling pathways, including Bfar, Itpr1, Srpx, Hspa1b, Usp25, Jun, Casp12, Eif2ak2, Casp4, Trim25, Ifng, Atf3, Hspa1a, Ppp1r15a, and Creb3. Through analysis with the MCODE plugin in Cytoscape, six genes, Atf3, Ppp1r15a, Casp12, Eif2ak2, Ifng, and Hspa1a, were identified as hub genes by the K-means algorithm from Cluster 1. In the in vivo MIRI model, the six genes mentioned above exhibited significant mRNA overexpression in the left anterior descending coronary artery ligation (LAD) model in rats. Conclusions: Altogether, the present study identified six potential DEGs involved in ERS induced by MIRI, which could be therapeutic targets in dealing with IRI-related ERS.","PeriodicalId":55162,"journal":{"name":"European Journal of Inflammation","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41968745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-26DOI: 10.1177/1721727x231172836
Min-Hyeok An, Pureun-Haneul Lee, Seon-Muk Choi, DaYeon Hwang, Shin-Ja Park, A. Baek, A. Jang
Objectives: Erdosteine, an oral mucoactive anti-oxidant molecule, interferes with the pathological processes seen in respiratory disorders including thickened or increased mucus production, increased oxidative stress, and chronic inflammation; however, its efficacy as an asthma therapy remains to be fully clarified. Therefore, the aim of this study was to assess the effects of erdosteine on epithelial barrier function in asthma. Methods: To investigate the effects of erdosteine on cell barrier expression in a mouse model of asthma, BALB/c mice ( n = 8 per group; total of 40 mice) were exposed to saline (Sham), ovalbumin (OVA), or OVA plus TiO2 inhalation (200 μg/m3; OVA + TiO2). The mice were then treated with erdosteine orally (OVA + TiO2 + Erdos) or intraperitoneal dexamethasone (OVA + TiO2 + Dex). Bronchoalveolar lavage and histology were performed. Enhanced pause was used as an indicator of pulmonary function, and samples were collected. The effect of erdosteine on cell barrier expression was assessed by immunoblotting and immunohistochemical analyses. Results: OVA + TiO2 + erdosteine mice exhibited decreased inflammation, and mucous gland hyperplasia, and increased pulmonary function compared with OVA + TiO2 mice. Levels of claudin (CLDN)-4 and nectin-4 protein were higher in lung tissue from OVA + TiO2 mice than Sham and OVA mice, and were reduced by erdosteine treatment. In contrast, CLDN14 and CLDN18 protein levels were lower in lung tissue from OVA + TiO2 mice than those from control mice, but were increased by treatment with erdosteine. Conclusion: Cell barriers are involved in airway inflammation in asthma patients, and erdosteine reduces airway inflammation by changing the cell barrier.
{"title":"Erdosteine effects cell barriers causing decreased inflammation and increased pulmonary function in asthmatic mice exposed to nanoparticulate pollution","authors":"Min-Hyeok An, Pureun-Haneul Lee, Seon-Muk Choi, DaYeon Hwang, Shin-Ja Park, A. Baek, A. Jang","doi":"10.1177/1721727x231172836","DOIUrl":"https://doi.org/10.1177/1721727x231172836","url":null,"abstract":"Objectives: Erdosteine, an oral mucoactive anti-oxidant molecule, interferes with the pathological processes seen in respiratory disorders including thickened or increased mucus production, increased oxidative stress, and chronic inflammation; however, its efficacy as an asthma therapy remains to be fully clarified. Therefore, the aim of this study was to assess the effects of erdosteine on epithelial barrier function in asthma. Methods: To investigate the effects of erdosteine on cell barrier expression in a mouse model of asthma, BALB/c mice ( n = 8 per group; total of 40 mice) were exposed to saline (Sham), ovalbumin (OVA), or OVA plus TiO2 inhalation (200 μg/m3; OVA + TiO2). The mice were then treated with erdosteine orally (OVA + TiO2 + Erdos) or intraperitoneal dexamethasone (OVA + TiO2 + Dex). Bronchoalveolar lavage and histology were performed. Enhanced pause was used as an indicator of pulmonary function, and samples were collected. The effect of erdosteine on cell barrier expression was assessed by immunoblotting and immunohistochemical analyses. Results: OVA + TiO2 + erdosteine mice exhibited decreased inflammation, and mucous gland hyperplasia, and increased pulmonary function compared with OVA + TiO2 mice. Levels of claudin (CLDN)-4 and nectin-4 protein were higher in lung tissue from OVA + TiO2 mice than Sham and OVA mice, and were reduced by erdosteine treatment. In contrast, CLDN14 and CLDN18 protein levels were lower in lung tissue from OVA + TiO2 mice than those from control mice, but were increased by treatment with erdosteine. Conclusion: Cell barriers are involved in airway inflammation in asthma patients, and erdosteine reduces airway inflammation by changing the cell barrier.","PeriodicalId":55162,"journal":{"name":"European Journal of Inflammation","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43254504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
To evaluate the correlation of miRNA-related single nucleotide polymorphisms (miR-SNPs) with the risk of dermatomyositis (DM) development. MicroRNAs (MiRNAs) are involved in a variety of activities such as cell differentiation, proliferation, apoptosis, tumorigenesis, and immunological response. MiR-SNPs alter the expression levels of miRNAs, leading to increased susceptibility to DM. We genotyped six miR-SNPs for miRNA processing machinery genes, including XPO5 (rs11077), RAN (rs14035), Dicer (rs3742330), TNRC6B (rs9623117), GEMIN3 (rs197412), and GEMIN4 (rs2740348), and two miR-SNPs for microRNA binding site, including SET8 (rs16917496), and KRT81 (rs3660), in a case-control study to assess the impact of these miR-SNPs on DM risk. Then we assessed cytokine expression and ROS levels in DM to determine the relationship between risk-related miR-SNPs and cytokines. We discovered that Dicer’s (rs3742330) AA genotype had a decreased chance of developing DM than the AG + GG type (odds ratio, 0.527; 95% confidence interval: 0.281–0.987; p = 0.045). The subsequent analysis showed that the AA genotype carrier had greater levels of IL-4 ( p = 0.034). The SNP of Dicer (rs3742330) maybe an attractive predictor of DM, moreover the cytokine of IL-4 may act as the factor that distinguishes SNP of Dicer (rs3742330) into AA and AG + GG.
{"title":"A microRNA-related single nucleotide polymorphism of the Dicer gene is associated with risk of dermatomyositis","authors":"Chenxing Peng, Jingjing Zhang, Shasha Zhang, Xiaoyun Zhang, Yufei Zhao","doi":"10.1177/1721727x231173526","DOIUrl":"https://doi.org/10.1177/1721727x231173526","url":null,"abstract":"To evaluate the correlation of miRNA-related single nucleotide polymorphisms (miR-SNPs) with the risk of dermatomyositis (DM) development. MicroRNAs (MiRNAs) are involved in a variety of activities such as cell differentiation, proliferation, apoptosis, tumorigenesis, and immunological response. MiR-SNPs alter the expression levels of miRNAs, leading to increased susceptibility to DM. We genotyped six miR-SNPs for miRNA processing machinery genes, including XPO5 (rs11077), RAN (rs14035), Dicer (rs3742330), TNRC6B (rs9623117), GEMIN3 (rs197412), and GEMIN4 (rs2740348), and two miR-SNPs for microRNA binding site, including SET8 (rs16917496), and KRT81 (rs3660), in a case-control study to assess the impact of these miR-SNPs on DM risk. Then we assessed cytokine expression and ROS levels in DM to determine the relationship between risk-related miR-SNPs and cytokines. We discovered that Dicer’s (rs3742330) AA genotype had a decreased chance of developing DM than the AG + GG type (odds ratio, 0.527; 95% confidence interval: 0.281–0.987; p = 0.045). The subsequent analysis showed that the AA genotype carrier had greater levels of IL-4 ( p = 0.034). The SNP of Dicer (rs3742330) maybe an attractive predictor of DM, moreover the cytokine of IL-4 may act as the factor that distinguishes SNP of Dicer (rs3742330) into AA and AG + GG.","PeriodicalId":55162,"journal":{"name":"European Journal of Inflammation","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44654610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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